Sequencing chip positioning structure and gene sequencer
By designing the sequencing chip positioning structure used in gene sequencers and adjusting the position of the gene sequencing chip using airflow, the problem of inaccurate positioning of the gene sequencing chip in the prior art is solved, automatic precise positioning is achieved, and the reliability and quality of sequencing data are improved.
Patent Information
- Application Number
- CN202421497788.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-27
- Publication Date
- 2025-05-27
- Estimated Expiration
- 2034-06-27
AI Technical Summary
The existing second-generation gene sequencers have high requirements for placement accuracy of gene sequencing chips, but there is an error rate of about 5% during operation, resulting in reagent loading failure or leakage, increasing the risk of sequencing failure and affecting the quality of sequencing data.
Design a sequencing chip positioning structure, including a cylindrical body, air inlet and air blowing port, adjust the position of the gene sequencing chip through the airflow channel, and achieve automatic and accurate positioning.
Through automatic and precise positioning, the risk of reagent loading failure or leakage is reduced, the reliability and quality of sequencing data is improved, and the operational complexity is reduced.
Smart Images
Figure CN222907907U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of gene sequencing, in particular to a positioning structure for a sequencing chip and a gene sequencer. Background Art
[0002] The working principle of a next-generation gene sequencer (NGS) is to load a sample onto a gene sequencing chip, perform a series of biochemical reactions (such as excision, synthesis, temperature rise and fall, etc.), then take a photo, and convert the fluorescence signal into an electrical signal for reading. Among them, reagent loading, temperature rise and fall of the gene sequencing chip, etc. need to be carried out on a supporting platform.
[0003] In the prior art, when a next-generation gene sequencer performs sequencing, it is usually necessary to manually place the gene sequencing chip on the platform, and the position accuracy requirement reaches about 0.05 mm. However, in the actual operation process, there is an error rate of about 5% in the accuracy of the placement position of the gene sequencing chip, and the position of the gene sequencing chip often shifts due to accidents during operation, such as the gene sequencing chip moving when the hand is released. An inaccurate placement position of the gene sequencing chip will lead to reagent loading failure or liquid leakage, thus increasing the risk of sequencing failure, or inaccurate positioning during photo-taking, thus affecting the quality of sequencing data. Summary of the Utility Model
[0004] The purpose of the utility model is to provide a positioning structure for a sequencing chip that can effectively and accurately automatically position the placement position of a gene sequencing chip and a gene sequencer including the positioning structure for a sequencing chip.
[0005] To achieve the above purpose, the technical solution of the utility model is as follows:
[0006] A positioning structure for a sequencing chip, including a cylindrical body, an air inlet and an air blowing port provided at opposite ends of the body;
[0007] An air flow channel is provided in the body, and the air flow channel is respectively communicated with the air inlet and the air blowing port;
[0008] The air inlet is used to connect to a gas source;
[0009] The air blowing port is used to align with the gene sequencing chip to be positioned, and the position of the gene sequencing chip is adjusted to the target positioning position through air flow.
[0010] Further, the body includes an open end and an opposite closed end;
[0011] The air inlet is provided at the open end; and / or, the air inlet is provided at the circumferential side portion of the open end of the body;
[0012] The air blowing port is provided at the circumferential side portion of the closed end.
[0013] Further, the air blowing port is strip-shaped, and the projection length of the air blowing port in the height direction of the gene sequencing chip is at least not less than the height of the gene sequencing chip.
[0014] Further, the length direction of the air blowing port is parallel to the axis in the height direction of the gene sequencing chip;
[0015] Or, the length direction of the air blowing port is arranged at an angle with the axis in the height direction of the gene sequencing chip.
[0016] Further, the width range of the air blowing port is 0.05 mm - 0.5 mm.
[0017] On the other hand, the present utility model provides a gene sequencer, comprising:
[0018] A chip carrier for placing a gene sequencing chip;
[0019] A plurality of sequencing chip positioning structures as described in any one of the above embodiments, respectively arranged on different sides of the gene sequencing chip, and the positions of the gene sequencing chip on the chip carrier are adjusted to target positioning positions by the airflow blown out through the air blowing ports of the plurality of sequencing chip positioning structures.
[0020] Further, the gene sequencer further comprises:
[0021] A plurality of abutting pins, respectively arranged on a group of adjacent sides of the gene sequencing chip for pre-positioning the gene sequencing chip; the plurality of sequencing chip positioning structures are arranged on the other group of adjacent sides of the gene sequencing chip, and the position of the gene sequencing chip on the chip carrier is automatically adjusted by the airflow blown out through the air blowing ports until it abuts against the abutting pins; or,
[0022] The plurality of sequencing chip positioning structures are respectively arranged on the four sides of the gene sequencing chip. Among them, a group of adjacent sides of the sequencing chip positioning structures are used as abutting pins for pre-positioning the gene sequencing chip, and the other group of adjacent sides of the sequencing chip positioning structures are used as positioning pins, and the position of the gene sequencing chip on the chip carrier is automatically adjusted by the airflow blown out through the air blowing ports until it abuts against the abutting pins.
[0023] Further, the top end face of the abutting pin is higher than the upper surface of the gene sequencing chip;
[0024] And / or, the top end face of the sequencing chip positioning structure is flush with or lower than the upper surface of the gene sequencing chip.
[0025] Further, a liquid inlet manifold block and a liquid outlet manifold block are respectively arranged on two opposite sides of the chip carrier. The gene sequencing chip is placed above the chip carrier, the liquid inlet manifold block and the liquid outlet manifold block through vacuum adsorption.
[0026] The liquid inlet manifold block and / or the liquid outlet manifold block are connected to the chip carrier through the sequencing chip positioning structure.
[0027] Further, a protective frame is arranged on the outer periphery of the gene sequencing chip, and avoidance holes are arranged at positions corresponding to the sequencing chip positioning structure on the protective frame.
[0028] And / or, the thrust applied to the gene sequencing chip by the air flow blown out from each sequencing chip positioning structure through the air blowing port is greater than the static friction force between the gene sequencing chip and the chip carrier.
[0029] Compared with the prior art, the sequencing chip positioning structure provided by the embodiment of the present utility model at least has the following technical effects:
[0030] The sequencing chip positioning structure includes a main body, an air inlet and an air blowing port arranged at opposite ends of the main body. Among them, an air flow channel is arranged in the main body, and the air flow channel is respectively communicated with the air inlet and the air blowing port. The air inlet is used to connect to an air source, and the air blowing port is used to align with the gene sequencing chip to be positioned. The sequencing chip positioning structure adjusts the position of the gene sequencing chip by blowing out air flow to move it to the target positioning position, so that the gene sequencing chip realizes automatic and accurate positioning, avoiding reagent loading failure or liquid leakage caused by inaccurate positioning, thereby reducing the risk of sequencing failure. And in the subsequent photographing step of gene sequencing, accurate positioning makes the acquisition of image data more accurate, and thus more reliable sequencing data quality is obtained.
[0031] The gene sequencer includes the sequencing chip positioning structure in the above embodiment, so it at least has the technical effects corresponding to each embodiment of the sequencing chip positioning structure, which will not be elaborated here. In the gene sequencer, the sequencing chip positioning structure can replace ordinary fastening pins, so that while realizing fastening parts, it can also realize automatic positioning of the gene sequencing chip, realizing the function of one thing with two uses. Thus, the gene sequencer can realize the automatic positioning function without changing the original structure, and can effectively reduce costs. BRIEF DESCRIPTION OF THE DRAWINGS
[0032] Figure 1 It is a schematic diagram of the overall structure of the sequencing chip positioning structure in an embodiment;
[0033] Figure 2 For Figure 1 side view;
[0034] Figure 3Schematic structural diagram of a gene sequencer in an embodiment;
[0035] Figure 4 is Figure 3 exploded structural diagram of;
[0036] Figure 5 Schematic structural diagram of the distribution of the abutting pin and the sequencing chip positioning structure on the four sides of the gene sequencing chip in an embodiment.
[0037] Explanation of the reference numerals in the drawings:
[0038] 10. Sequencing chip positioning structure; 11. Body; 12. Air inlet; 121. Open end; 13. Blowing port; 131. Closed end; 20. Gene sequencing chip; 30. Chip carrier; 31. Liquid inlet manifold block; 32. Liquid outlet manifold block; 33. Protection frame; 331. Avoidance hole; 40. Abutting pin; 50. Positioning pin. Detailed implementation manners
[0039] The technical solution of the present utility model will be further elaborated in detail below in conjunction with the drawings in the specification and specific embodiments. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present utility model belongs. The terms used in the specification of the present utility model herein are only for the purpose of describing specific embodiments and are not intended to limit the present utility model. In the following description, the expression "some embodiments" is described, which describes a subset of all possible embodiments. However, it should be understood that "some embodiments" can be the same subset or different subsets of all possible embodiments and can be combined with each other without conflict.
[0040] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or there may also be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "inner", "outer", "left", "right" and similar expressions used herein are only for the purpose of illustration and do not represent the only implementation manner.
[0041] Please refer to the attached Figure 1 and the attached Figure 2, an embodiment of the present utility model provides a sequencing chip positioning structure. The sequencing chip positioning structure 10 includes a cylindrical body 11, an air inlet 12 and a blowing port 13 provided at opposite ends of the body 11; an air flow channel is provided in the body 11, and the air flow channel is respectively communicated with the air inlet 12 and the blowing port 13; the air inlet 12 is used to connect to a gas source; the blowing port 13 is used to align with the gene sequencing chip 20 to be positioned, and the position of the gene sequencing chip 20 is adjusted to the target positioning position through the air flow.
[0042] Among them, the body 11 of the sequencing chip positioning structure 10 is cylindrical, but its shape is not limited to this, and it can be designed into other shapes according to specific requirements, such as a square column shape. The air inlet 12 is provided at one end of the body 11 and is used to connect various gas source systems, such as a micro air pump. The micro air pump can select various known air pump products according to the required air flow pressure. In an optional example, the blowing air flow pressure of the blowing port 13 of the sequencing chip positioning structure 10 is estimated according to the weight of the gene sequencing chip and the friction force when the gene sequencing chip moves on the platform. For example, the weight of the gene sequencing chip is about 0.02 kg, and the friction coefficient is about 0.75 according to the weight and material of the gene sequencing chip and the material of the platform carrying the gene sequencing chip, and the static friction force between the two can be determined. In this way, the thrust force generated by the blowing air flow of the blowing port 13 of the sequencing chip positioning structure 10 can be calculated, and thus the pressure provided by the micro air pump can also be determined, such as the continuous positive pressure of the micro air pump is 0.6 Mpa. After the gas source system is started, the air flow enters the air flow channel through the air inlet 12, flows through the air flow channel to the blowing port 13, and the air flow blows out from the blowing port 13 and aligns with the gene sequencing chip 20 to be positioned. The driving force of the air flow makes the gene sequencing chip 20 move on the platform and gradually adjusts the position to the target positioning position.
[0043] The sequencing chip positioning structure 10 of this embodiment includes a body 11 and an air inlet 12 and a blowing port 13 provided at opposite ends of the body 11. Among them, an air flow channel is provided in the body 11, and the air flow channel is respectively communicated with the air inlet 12 and the blowing port 13. The air inlet 12 is used to connect to a gas source, and the blowing port 13 is used to align with the gene sequencing chip 20 to be positioned. The position of the gene sequencing chip 20 is adjusted to the target positioning position through the air flow, so that the gene sequencing chip 20 realizes automatic and accurate positioning, avoiding reagent loading failure or liquid leakage caused by inaccurate positioning, thereby reducing the risk of sequencing failure. Moreover, in the subsequent photographing step of gene sequencing, accurate positioning enables more accurate image acquisition, and thus more efficient test image data can be obtained.
[0044] In some alternative embodiments, the body 11 includes an open end 121 and an opposite closed end 131; the air inlet 12 is provided at the open end 121 and can be directly connected to an external air source; and / or, the air inlet 12 is provided at the circumferential side of the open end 121 of the body 11; the air blowing port 13 is provided at the circumferential side of the closed end 131.
[0045] In some alternative embodiments, the air blowing port 13 is strip-shaped, and the projection length of the air blowing port 13 in the height direction of the gene sequencing chip 20 is at least not less than the height of the gene sequencing chip 20, ensuring that the air flow can cover the entire height range of the gene sequencing chip 20 and provide a uniform and stable air flow in the entire height direction of the gene sequencing chip 20. It should be further noted that when the gene sequencing chip 20 is installed, there are situations where it sinks and does not sink due to adsorption. In a specific example, the projection length of the air blowing port 13 in the height direction of the gene sequencing chip 20 is greater than the height of the gene sequencing chip 20. For example, the height of the gene sequencing chip 20 is 1 mm and it sinks 1 mm during adsorption. If the projection length of the air blowing port 13 in the height direction of the gene sequencing chip 20 is 2 mm, it can just cover the sinking distance, ensuring that the air blowing port 13 still corresponds to the gene sequencing chip 20 in the height direction after sinking and ensuring the accurate positioning of the gene sequencing chip 20. In another specific example, the projection length of the air blowing port 13 in the height direction of the gene sequencing chip 20 is the same as the height of the gene sequencing chip 20. When the gene sequencing chip 20 is installed without sinking, the two ends of the projection length of the air blowing port 13 in the height direction of the gene sequencing chip 20 are flush with the upper surface and the lower surface of the gene sequencing chip 20 respectively, ensuring that the gene sequencing chip 20 is subjected to a stable air flow pressure throughout the height, thereby improving the effectiveness of the air blowing of the air blowing port 13 on the gene sequencing chip 20 and avoiding waste of the air flow.
[0046] In some alternative embodiments, the length direction of the air blowing port 13 is parallel to the axis in the height direction of the gene sequencing chip 20; or, the length direction of the air blowing port 13 is arranged at an angle with the axis in the height direction of the gene sequencing chip 20, and the angle range can be 0° - 90°.
[0047] In some alternative embodiments, the width range of the air blowing port 13 is 0.05 mm - 0.5 mm. According to the principle of fluid dynamics, the smaller the width of the air blowing port 13, the greater the speed of the blown air flow. Therefore, by selecting a smaller width of the air blowing port 13, the air flow speed can be significantly increased. Preferably, the width of the air blowing port 13 is 0.1 mm.
[0048] On the other hand, please refer to the attached Figure 3 and the attached Figure 4, an embodiment of the present utility model provides a gene sequencer, which includes a chip carrier 30 and a plurality of sequencing chip positioning structures in the above embodiments. The chip carrier 30 is used to place the gene sequencing chip 20. The plurality of sequencing chip positioning structures 10 are respectively arranged on different sides of the gene sequencing chip 20. The plurality of sequencing chip positioning structures 10 adjust the position of the gene sequencing chip 20 on the chip carrier 30 to the target positioning position by blowing air through the air blowing ports 13. The gene sequencer includes the sequencing chip positioning structure in the above embodiment, so it at least has the technical effects corresponding to the embodiment of the sequencing chip positioning structure, which will not be elaborated here. In addition, the sequencing chip positioning structure 10 can be used to replace ordinary fastening pins, so that while fastening parts, it can automatically position the gene sequencing chip 20, realizing the function of one thing for two uses. Thus, the gene sequencer can realize the automatic positioning function without changing the original structure and can effectively reduce costs.
[0049] Please refer to the attached Figure 5 , in some alternative embodiments, the plurality of sequencing chip positioning structures 10 are respectively arranged on the four sides of the gene sequencing chip 20. Among them, a group of sequencing chip positioning structures 10 on two adjacent sides do not blow air and are used as abutting pins 40 for pre-positioning the gene sequencing chip 20. Another group of sequencing chip positioning structures 10 on the other two adjacent sides blow air and are used as positioning pins 50. The air flow is blown through the air blowing ports 13 to automatically adjust the position of the gene sequencing chip 20 on the chip carrier 30 until it abuts against the abutting pins 40. The gene sequencing chip 20 is usually rectangular, including a first side, a second side, a third side, and a fourth side. The first side is adjacent to the second side, the second side is adjacent to the third side, and the third side is adjacent to the fourth side. Among them, the plurality of sequencing chip positioning structures 10 are respectively arranged on the first side, the second side, the third side, and the fourth side of the gene sequencing chip 20. In a specific example, the sequencing chip positioning structures 10 arranged on the first side and the second side do not blow air and are used as abutting pins 40, and do not blow air when performing the pre-positioning function. Correspondingly, the sequencing chip positioning structures 10 arranged on the third side and the fourth side blow air and are used as positioning pins 50. The air blowing ports 13 blow out air flow, and the driving force of the air flow can automatically adjust the position of the gene sequencing chip 20 on the chip carrier 30 to make it fit with the abutting pins 40, so as to achieve precise positioning. In addition, the sequencing chip positioning structure 10 can play a role in initial positioning for the initial placement of the gene sequencing chip 20, avoiding the gene sequencing chip 20 being too far away from the sequencing chip positioning structure 10 and resulting in the inability to blow the gene sequencing chip 20.
[0050] In some other alternative embodiments, the gene sequencer further includes a plurality of abutting pins 40, which are respectively arranged on a set of adjacent sides of the gene sequencing chip 20 for pre-positioning the gene sequencing chip 20; a plurality of sequencing chip positioning structures 10 are arranged on the other set of adjacent sides of the gene sequencing chip 20, and the air outlet 13 blows out air to automatically adjust the position of the gene sequencing chip 20 on the chip carrier 30 until it abuts against the abutting pins 40; specifically, the abutting pins 40 can be ordinary pins or the sequencing chip positioning structures 10 in the above embodiments, and no specific limitation is made here. When the abutting pins 40 are the sequencing chip positioning structures 10 in the above embodiments, the sequencing chip positioning structures 10 do not perform the air blowing operation when performing the pre-positioning function. In a specific embodiment, the gene sequencer includes a plurality of abutting pins 40 and a plurality of sequencing chip positioning structures 10 for accurately positioning the gene sequencing chip 20. Specifically, a plurality of abutting pins 40 are respectively arranged on the first side and the second side for preliminary pre-positioning of the gene sequencing chip 20. A plurality of sequencing chip positioning structures 10 are respectively arranged on the third side and the fourth side. The sequencing chip positioning structures 10 are equipped with air outlets 13. The air outlets 13 blow out air, and the driving force of the air flow can automatically adjust the position of the gene sequencing chip 20 on the chip carrier 30 to make it fit with the abutting pins 40, so as to achieve accurate positioning.
[0051] In some alternative embodiments, the top end face of the abutting pin 40 is higher than the upper surface of the gene sequencing chip 20, which is convenient for the gene sequencing chip 20 to better abut against the abutting pin 40 during positioning. Specifically, when the gene sequencing chip 20 is positioned, the abutting pin 40 provides a physical stop for the gene sequencing chip 20 to ensure accurate positioning. In addition, the top end face of the positioning pin 50 is flush with or lower than the upper surface of the gene sequencing chip 20, which helps the gene sequencing chip 20 to be more conveniently placed on the chip carrier 30.
[0052] In some alternative embodiments, after multiple abutting pins 40 pre-position the gene sequencing chip 20 respectively, the distance range between the sequencing chip positioning structure 10 serving as the positioning pin 50 and the edge of the gene sequencing chip 20 is 0.1 mm - 0.5 mm. This design enables the gene sequencing chip 20 to be placed on the chip carrier 30 more conveniently, and at the same time better ensures the strength of the airflow blown out from the air outlet 13. It should be further noted that the abutting pins 40 and the positioning pins 50 are arranged at key positions of the gene sequencing chip 20 to ensure the precise positioning of the gene sequencing chip 20. Specifically, when the gene sequencing chip 20 is rectangular with a long side and a short side, two or more abutting pins 40 can be arranged at intervals on one side of the long side, two or more positioning pins 50 are correspondingly arranged on the other side of the long side, the number of abutting pins 40 arranged on one side of the short side is less than that on one side of the long side, and positioning pins 50 are correspondingly arranged on the other side of the short side.
[0053] In some alternative embodiments, a liquid inlet manifold block 31 and a liquid outlet manifold block 32 are respectively arranged on opposite sides of the chip carrier 30. The gene sequencing chip 20 is placed above the chip carrier 30, the liquid inlet manifold block 31 and the liquid outlet manifold block 32 through vacuum adsorption; the liquid inlet manifold block 31 and / or the liquid outlet manifold block 32 are connected to the chip carrier 30 through the sequencing chip positioning structure 10. Among them, the sequencing chip positioning structure 10 can be used to fix the liquid inlet manifold block 31 and / or the liquid outlet manifold block 32, and at the same time can automatically position the gene sequencing chip 20, enabling the sequencing chip positioning structure 10 to realize the function of serving two purposes. On the basis of not adding other additional components except for the air pump and its accessories, the automatic positioning function is realized, which can effectively reduce the cost and the increase in the overall occupied space is small. Specifically, the sequencing chip positioning structure 10 is arranged on the outside of the gene sequencing chip 20 for the liquid inlet manifold block 31 and on the outside of the gene sequencing chip 20 for the liquid outlet manifold block 32. In a specific example, a first abutting pin and a first positioning pin are respectively arranged at opposite ends of the liquid inlet manifold block 31, a second abutting pin and a second positioning pin are respectively arranged at opposite ends of the liquid outlet manifold block 32, a third abutting pin is arranged between the first abutting pin and the first positioning pin, and a third abutting pin is arranged between the second abutting pin and the second positioning pin; the first abutting pin and the second abutting pin are arranged on the same side, the first positioning pin and the second positioning pin are arranged on the same side, and the first abutting pin and the second abutting pin are arranged in one-to-one correspondence with the first positioning pin and the second positioning pin, and the third abutting pin is arranged in correspondence with the third positioning pin. When installing the gene sequencing chip 20, after the user places the gene sequencing chip 20 on the chip carrier 30, while pressing the bottom surface vacuum adsorption switch, multiple positioning pins 50 start to eject high-pressure airflows, pushing the gene sequencing chip 20 to abut against the abutting pins 40 for positioning, and completing the process of positioning and adsorption of the gene sequencing chip 20.
[0054] In some alternative embodiments, a protective frame 33 is provided on the outer periphery of the gene sequencing chip 20, and an avoidance hole 331 is provided at a position corresponding to the sequencing chip positioning structure 10; it should be further noted that the gene sequencing chip 20 can be, but is not limited to, a glass slide or a silicon wafer etched with a flow cell. The sequencing chip positioning structure 10 passes through the avoidance hole 331 on the protective frame 33 to automatically position the gene sequencing chip 20. The sequencing chip positioning structure 10 only acts on the gene sequencing chip 20 and does not affect the protective frame 33. The sequencing chip positioning structure 10 can also be used for the automatic positioning of the gene sequencing chip 20 without the protective frame 33.
[0055] In some alternative embodiments, the thrust applied by the air flow blown out by each sequencing chip positioning structure 10 through the air blowing port 13 to the gene sequencing chip 20 is greater than the static friction force between the gene sequencing chip 20 and the chip carrier 30. The theoretically calculated stable thrust of the air flow is as follows: F = (volume flow rate × air density × air flow velocity) / 550, where the air flow velocity cannot be directly calculated. Generally, the smaller the cross-sectional area of the air blowing port 13, the greater the air flow velocity. Through ANSYS simulation, the thrust generated by the air flow blown out by a single air blowing port 13 is 0.219 + 0.039 + 0.258 ≈ 0.52 N. The mass of the gene sequencing chip 20 is about 0.02 kg, and the friction coefficient between it and the chip carrier 30 is 0.75 (referring to the static friction coefficient between glass and rubber, and the sealing ring material at the inlet and outlet of the gene sequencing chip 20 is rubber). Therefore, the static friction force between the gene sequencing chip 20 and the chip carrier 30 is 0.15 N, so that the thrust generated by the air flow blown out by the sequencing chip positioning structure 10 can blow the gene sequencing chip 20.
[0056] The above are only the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of changes or substitutions, which should all be covered within the protection scope of the present invention. The protection scope of the present invention shall be subject to the protection scope of the claims.
Claims
1. A sequencing chip positioning structure, characterized in that: It comprises a cylindrical body, an air inlet and an air blowing port arranged at opposite ends of the body; An air flow channel is provided in the body, and the air flow channel is communicated with the air inlet and the air blowing port respectively; The air inlet is used to connect to an air source; The air outlet is used to align with the gene sequencing chip to be positioned, and the position of the gene sequencing chip is adjusted to the target positioning position through airflow.
2. The sequencing chip positioning structure according to claim 1, characterized in that: The body includes an open end and an opposite closed end; The air inlet is arranged at the opening end; and / or, the air inlet is arranged at the circumferential side of the opening end of the body; The blowing port is arranged at the circumferential side of the closed end.
3. The sequencing chip positioning structure according to claim 1, characterized in that: The air outlet is in the shape of an elongated strip, and the projection length of the air outlet in the height direction of the gene sequencing chip is at least not less than the height of the gene sequencing chip.
4. The sequencing chip positioning structure according to claim 3, characterized in that: The length direction of the air outlet is parallel to the axis of the gene sequencing chip in the height direction; Alternatively, the length direction of the air outlet is arranged to form an angle with the axis in the height direction of the gene sequencing chip.
5. The sequencing chip positioning structure according to claim 3, characterized in that: The width of the blowing port is in the range of 0.05 mm to 0.5 mm.
6. A gene sequencer, characterized in that: include: A chip carrier, used to place a gene sequencing chip; A plurality of sequencing chip positioning structures as described in any one of claims 1 to 5 are respectively arranged on different sides of the gene sequencing chip, and the plurality of sequencing chip positioning structures adjust the position of the gene sequencing chip on the chip carrier to a target positioning position by blowing out airflow through the air outlet.
7. The gene sequencer according to claim 6, characterized in that: Also includes: A plurality of close-fitting pins are respectively arranged on two adjacent sides of a group of the gene sequencing chip, and are used to pre-position the gene sequencing chip; A plurality of the sequencing chip positioning structures are arranged on two adjacent sides of another group of the gene sequencing chip, and the airflow blown out of the air outlet automatically adjusts the position of the gene sequencing chip on the chip carrier to abut against the abutting pin; or, A plurality of the sequencing chip positioning structures are respectively arranged on the four sides of the gene sequencing chip, wherein a group of the sequencing chip positioning structures on the two adjacent sides are not blown with air and are used as abutment pins for pre-positioning the gene sequencing chip, and another group of the sequencing chip positioning structures on the two adjacent sides are blown with air and are used as positioning pins, and the airflow blown out through the air port automatically adjusts the position of the gene sequencing chip on the chip carrier until it abuts against the abutment pins.
8. The gene sequencer according to claim 7, characterized in that: The top end surface of the pin is higher than the upper surface of the gene sequencing chip; And / or, the top end surface of the sequencing chip positioning structure is flush with or lower than the upper surface of the gene sequencing chip.
9. The gene sequencer according to claim 6, characterized in that: The chip carrier is provided with a liquid inlet manifold and a liquid outlet manifold on opposite sides thereof, and the gene sequencing chip is placed on the chip carrier, the liquid inlet manifold and the liquid outlet manifold by vacuum adsorption; The liquid inlet manifold and / or the liquid outlet manifold are connected to the chip carrier through the sequencing chip positioning structure.
10. The gene sequencer according to claim 6, characterized in that: A protective frame is provided on the periphery of the gene sequencing chip, and an avoidance hole is provided at a position of the protective frame corresponding to the positioning structure of the sequencing chip; And / or, the thrust applied to the gene sequencing chip by the airflow blown out by each sequencing chip positioning structure through the air outlet is greater than the static friction between the gene sequencing chip and the chip carrier.