Cell counting plate integrating sample loading, dyeing and counting
By designing a cell counting plate that integrates loading, staining and counting, the problems of cumbersome operation and counting error of traditional methods are solved, and the efficiency and accuracy of cell counting are achieved.
Patent Information
- Application Number
- CN202421760244.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-24
- Publication Date
- 2025-05-27
- Estimated Expiration
- 2034-07-24
AI Technical Summary
Traditional cell counting methods are cumbersome to operate, which can easily lead to cell loss and counting errors.
A cell counting plate integrating loading, staining and counting is designed, including a vacuum piston, substrate, staining tank and support mechanism. Through the cooperation of the staining tank, vacuum piston and substrate, cells are loaded, stained and counted.
The cell counting process is efficient and accurate, the operation process is simplified, the losses and errors in cell transfer are reduced, and the experimental efficiency is improved.
Smart Images
Figure CN222907909U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of biomedicine, and specifically relates to a cell counting plate integrating sample loading, staining and counting. Background Art
[0002] Cell counting is a basic experimental technique in biological and medical research, used to determine the number of cells in a given sample, and is one of the important steps in cell biology experiments. General counting methods include direct microscopic counting method, hemocytometer method, cell culture counting method, etc.
[0003] Traditional counting methods usually require multiple transfers of cell samples and complex sample loading and staining processes, which are prone to cell loss and counting errors. Therefore, a device that can simplify the operation process and improve the counting accuracy is needed; therefore, in view of the above problems, a cell counting plate integrating sample loading, staining and counting is proposed. Summary of the Utility Model
[0004] In order to make up for the deficiencies of the prior art and solve the problems of cumbersome operation and large errors in the prior art, the utility model proposes a cell counting plate integrating sample loading, staining and counting.
[0005] The technical solution adopted by the utility model to solve its technical problems is: a cell counting plate integrating sample loading, staining and counting according to the utility model includes a vacuum piston, and a substrate is fixedly connected to one side of the vacuum piston;
[0006] A counting area and a staining groove are arranged in the substrate, a suction port is opened on the surface of the substrate, and an observation window is arranged on the surface of the substrate;
[0007] The suction port, the counting area and the vacuum piston are all connected through the staining groove in a penetrating manner;
[0008] Dry acridine orange and DAPI staining agents are arranged in the staining groove;
[0009] A support mechanism is arranged on the surface of the substrate for strengthening the stability of the substrate.
[0010] Preferably, the counting area is a counting pool with a depth of 0.1 mm, and the counting pool is divided into several squares.
[0011] Preferably, the substrate is made of a transparent material, and the squares are divided into three types with side lengths of 1 mm, 0.05 mm and 0.2 mm.
[0012] Preferably, the support mechanism includes a rotating shaft rotatably connected to the surface of the substrate. A scraping rod is fixedly connected to the surface of the rotating shaft. A support block and a sponge block are fixedly connected to the surface of the scraping rod. There are two rotating shafts, and a pair of rotating shafts are symmetrically distributed on both sides of the substrate. Scraping rods are fixedly connected to the surfaces of a pair of rotating shafts.
[0013] Preferably, a fixing block is fixedly connected to the surface of the scraping rod. A connecting block is rotatably connected to one side of the fixing block. An inserting block is slidably connected to the connecting block through a spring. A plurality of teeth are fixedly connected to the surface of the substrate. Chamfers are provided at the positions where the teeth are in contact with the inserting block. The connecting block rotates at an angle of 90°.
[0014] Preferably, a first cavity and a second cavity are provided in the support block. An elastic block is fixedly connected to the first cavity. The elastic block is hollow. A push plate is slidably connected to the cavity. A sleeve is fixedly connected to one side of the scraping rod. An inserting rod is fixedly connected to one side of the connecting block. The end of the inserting rod away from the connecting block passes through the sleeve and is slidably connected to push the push plate to slide up and down. An elastic membrane is fixedly connected to the second cavity. A through groove is provided on the side wall of the second cavity. The elastic membrane and the elastic block are communicated through an air pipe.
[0015] Preferably, a rubber ring is fixedly connected to the sleeve. A runner is rotatably connected to the end of the inserting rod located in the first cavity. The push plate and the inserting rod are movably connected through the runner.
[0016] Preferably, anti-slip grooves are provided on the surface of the support block.
[0017] The beneficial effects of the present utility model are as follows:
[0018] 1. Through the cooperation of the dyeing tank, the vacuum piston and the substrate, the present utility model realizes the functions of integrated sample loading, dyeing and counting, and also realizes the high efficiency and accuracy of the cell counting process. It is applicable to various cell biology experiments and research. This design not only simplifies the operation process, but also improves the counting accuracy and experimental efficiency. At the same time, it also reduces the loss and error in the cell transfer process. The support structure can make the extrusion plate more stable to place and is more convenient for the staff to observe.
[0019] 2. When the elastic membrane expands, the sponge block bulges, which further improves the cleaning effect on the observation window and is more convenient for the staff to observe. Then when the scraping rod resets, the rubber ring in the sleeve can play a buffering role, slowing down the reset speed of the inserting rod, thereby ensuring the cleaning effect of the sponge block. Description of the Drawings
[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the attached drawings required for use in the description of the embodiments or the prior art. Obviously, the attached drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other attached drawings can be obtained based on these attached drawings.
[0021] Figure 1 Schematic diagram of the vacuum piston structure of Embodiment 1;
[0022] Figure 2 Schematic diagram of the dyeing tank structure of Embodiment 1;
[0023] Figure 3 Schematic diagram of the partial cross-sectional structure of the scraping rod of Embodiment 1;
[0024] Figure 4 For Embodiment 1 Figure 3 Schematic diagram of the structure at position A;
[0025] Figure 5 Schematic diagram of the elastic block structure of Embodiment 1;
[0026] Figure 6 Schematic diagram of the anti-slip groove structure of Embodiment 2.
[0027] In the figure: 1. Vacuum piston; 2. Rotating shaft; 3. Scraping rod; 4. Observation window; 5. Substrate; 6. Support block; 7. Suction port; 8. Dyeing tank; 9. Counting area; 10. Pushing plate; 11. Teeth; 12. Cavity 1; 13. Fixed block; 14. Connecting block; 15. Insert block; 16. Sleeve; 17. Insert rod; 18. Rubber ring; 19. Rotating wheel; 20. Through groove; 21. Cavity 2; 22. Elastic block; 23. Sponge block; 24. Elastic membrane; 25. Anti-slip groove. Detailed implementation manners
[0028] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the attached drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.
[0029] Embodiment 1
[0030] Please refer to Figures 1-5 As shown, a cell counting plate integrating sample loading, dyeing, and counting includes a vacuum piston 1, and a substrate 5 is fixedly connected to one side of the vacuum piston 1;
[0031] A counting area 9 and a staining tank 8 are arranged in the substrate 5. A suction port 7 is formed on the surface of the substrate 5, and an observation window 4 is arranged on the surface of the substrate 5.
[0032] The suction port 7, the counting area 9 and the vacuum piston 1 are all connected through the staining tank 8 in a penetrating manner.
[0033] Dry acridine orange and DAPI staining agents are arranged in the staining tank 8.
[0034] A support mechanism is arranged on the surface of the substrate 5 for strengthening the stability of the substrate 5. The counting area 9 is a counting pool with a depth of 0.1 mm, and the counting pool is divided into several squares. The substrate 5 is made of a transparent material, and the squares are divided into three types with side lengths of 1 mm, 0.05 mm and 0.2 mm.
[0035] During operation, the cell suspension is placed at the sample suction port 7. By pressing the vacuum piston 1, the cell sample is sucked in through the suction port 7 and enters the microfluidic staining tank 8. Dry acridine orange and DAPI staining agents are pre-placed in the staining tank 8. The cell sample passes through a curved path in the staining tank 8 to ensure sufficient staining. Subsequently, the stained cell sample enters the transparent cell counting area 9. The 0.1-mm counting pool is used in conjunction with a microscope for observation, and the cells in each small square can be accurately counted. At the same time, the substrate 5 is made of a highly transparent material, which is more convenient for observation. Through the cooperation of the staining tank 8, the vacuum piston 1 and the substrate 5, it has the functions of integrated sample loading, staining and counting, realizing the high efficiency and accuracy of the cell counting process, and is applicable to various cell biology experiments and research. This design not only simplifies the operation process, but also improves the counting accuracy and experimental efficiency. At the same time, it also reduces the loss and error during the cell transfer process. Through the support structure, the extrusion plate can be placed more stably, which is more convenient for the staff to observe.
[0036] The support mechanism includes a rotating shaft 2. The rotating shaft 2 is rotatably connected to the surface of the substrate 5. A scraping rod 3 is fixedly connected to the surface of the rotating shaft 2. A support block 6 is fixedly connected to the surface of the scraping rod 3. A sponge block 23 is fixedly connected to the surface of the scraping rod 3. There are two rotating shafts 2, and a pair of the rotating shafts 2 are symmetrically distributed on both sides of the substrate 5. A scraping rod 3 is fixedly connected to the surface of each pair of the rotating shafts 2. A fixing block 13 is fixedly connected to the surface of the scraping rod 3. A connecting block 14 is rotatably connected to one side of the fixing block 13. An inserting block 15 is slidably connected to the connecting block 14 through a spring. Oblique angles are formed at the positions where the inserting block 15 contacts the teeth 11 fixedly connected to the surface of the substrate 5. The rotating angle of the connecting block 14 is 90°.
[0037] During operation, when observing, the support block 6 is rotated around the rotating shaft 2, which will drive the scraping rod 3 to rotate at the same time. The scraping rod 3 drives the sponge block 23 to clean the surface of the substrate 5. After the scraping rod 3 is unfolded, the support block 6 levels the placement angle of the substrate 5, which is convenient for the staff to observe. When the scraping rod 3 rotates, it will drive the fixed block 13 to move, and at the same time, the fixed block 13 will drive the connecting block 14 to move, so that the connecting block 14 drives the internal insertion block 15 to contact the tooth 11. Through the oblique angles formed on the surfaces, they are mutually extruded, causing the insertion block 15 to retract inwardly into the connecting block 14. After passing through the first tooth 11, the spring drives the insertion block 15 to reset. At this time, the insertion block 15 will impact the substrate 5. By analogy, the rotation of the scraping rod 3 drives the insertion block 15 to impact the substrate 5, causing the substrate 5 to vibrate, making the cell distribution in the counting chamber more uniform, and further facilitating the staff to observe the internal cell conditions. When the scraping rod 3 rotates in reverse, the tooth 11 will push the connecting block 14, causing the connecting block 14 to rotate around the fixed crossbar, so as to facilitate the reset of the scraping rod 3. At the same time, the cooperation of the insertion block 15 and the tooth 11 can also position the scraping rod 3 to avoid random shaking, which is convenient for storage.
[0038] A cavity one 12 and a cavity two 21 are provided in the support block 6. An elastic block 22 is fixedly connected in the cavity one 12. The elastic block 22 is hollow. A push plate 10 is slidably connected in the cavity. One side of the scraping rod 3 is fixedly connected with a sleeve 16. One side of the connecting block 14 is fixedly connected with an insertion rod 17. The end of the insertion rod 17 away from the connecting block 14 passes through the sleeve 16 and is slidably connected. An elastic membrane 24 is fixedly connected in the cavity two 21. A through groove 20 is provided on the side wall of the cavity two 21. The elastic membrane 24 and the elastic block 22 are communicated through an air pipe. A rubber ring 18 is fixedly connected in the sleeve 16. The part of the insertion rod 17 located in the cavity one 12 is rotatably connected with a runner 19 at the end. The push plate 10 and the insertion rod 17 are movably connected through the runner 19;
[0039] During operation, the connecting block 14 drives the insertion rod 17 to slide in the sleeve rod, and at the same time, the insertion rod 17 drives the runner 19 to move, so that the runner 19 lifts the push plate 10. When the push plate 10 rises, it will squeeze the hollow elastic block 22 in the cavity one 12, so that the gas in the elastic block 22 is injected into the elastic membrane 24 through the air pipe, causing the elastic membrane 24 to expand. Through the through groove 20, the sponge block 23 is further driven to protrude. Through the protrusion of the sponge block 23, the cleaning effect on the observation window 4 can be further improved, which is more convenient for the staff to observe. Then when the scraping rod 3 resets, the rubber ring 18 in the sleeve 16 can play a buffering effect, slowing down the reset speed of the insertion rod 17, and thus ensuring the cleaning effect of the sponge block 23.
[0040] Embodiment Two
[0041] Please refer to Figure 6As shown, as another implementation manner of the present utility model compared with the first comparative example, anti-slip grooves 25 are formed on the surface of the support block 6; during operation, the anti-slip grooves 25 can increase the friction between the fingers of the staff and the support block 6, thereby avoiding the situation of slipping.
[0042] In the description of this specification, the description with reference to terms such as "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.
[0043] The above has shown and described the basic principles, main features and advantages of the present utility model. Those skilled in the art of this industry should understand that the present utility model is not limited by the above embodiments, and what is described in the above embodiments and the specification only illustrates the principle of the present utility model. Without departing from the spirit and scope of the present utility model, the present utility model will have various changes and improvements, and these changes and improvements all fall within the scope of the present utility model claimed.
Claims
1. A cell counting chamber integrating sample loading, staining and counting, characterized in that: It comprises a vacuum piston (1), one side of which is fixedly connected to a base plate (5); The substrate (5) is provided with a counting area (9) and a dyeing tank (8), a suction port (7) is provided on the surface of the substrate (5), and an observation window (4) is provided on the surface of the substrate (5); The suction port (7), the counting area (9) and the vacuum piston (1) are all connected through the dyeing tank (8); The staining tank (8) is provided with dry acridine orange and DAPI staining agents; A support mechanism is provided on the surface of the substrate (5) for supporting and strengthening the stability of the substrate (5).
2. A cell counting chamber integrating sample loading, staining and counting according to claim 1, characterized in that: The counting area (9) is a counting pool with a depth of 0.1 mm, and the counting pool is divided into a plurality of squares.
3. A cell counting chamber integrating sample loading, staining and counting according to claim 2, characterized in that: The substrate (5) is made of a transparent material, and the squares are divided into three types with side lengths of 1 mm, 0.05 mm and 0.2 mm.
4. A cell counting chamber integrating sample loading, staining and counting according to claim 3, characterized in that: The support mechanism comprises a rotating shaft (2), the rotating shaft (2) is rotatably connected to the surface of the base plate (5), a scraper rod (3) is fixedly connected to the surface of the rotating shaft (2), a support block (6) is fixedly connected to the surface of the scraper rod (3), and a sponge block (23) is fixedly connected to the surface of the scraper rod (3). Two rotating shafts (2) are provided, a pair of the rotating shafts (2) are symmetrically distributed on both sides of the base plate (5), and the surfaces of the pair of rotating shafts (2) are both fixedly connected to the scraper rods (3).
5. A cell counting chamber integrating sample loading, staining and counting according to claim 4, characterized in that: A fixed block (13) is fixedly connected to the surface of the scraper rod (3); a connecting block (14) is rotatably connected to one side of the fixed block (13); an inserting block (15) is slidably connected to the inside of the connecting block (14) via a spring; a plurality of latching teeth (11) are fixedly connected to the surface of the base plate (5); positions where the latching teeth (11) contact the inserting block (15) are provided with an oblique angle; and the connecting block (14) has a rotation angle of 90°.
6. The cell counting chamber integrating sample loading, staining and counting according to claim 5, characterized in that: The support block (6) is provided with a cavity one (12) and a cavity two (21). An elastic block (22) is fixedly connected to the cavity one (12). The elastic block (22) is hollow. A push plate (10) is slidably connected to the cavity. A sleeve (16) is fixedly connected to one side of the scraper rod (3). An insertion rod (17) is fixedly connected to one side of the connecting block (14). The end of the insertion rod (17) away from the connecting block (14) passes through the sleeve (16) for slidable connection and is used to push the push plate (10) to slide up and down. An elastic membrane (24) is fixedly connected to the cavity two (21). A through groove (20) is provided on the side wall of the cavity two (21). The elastic membrane (24) and the elastic block (22) are connected via an air pipe.
7. A cell counting chamber integrating sample loading, staining and counting according to claim 6, characterized in that: A rubber ring (18) is fixedly connected inside the sleeve (16); the insertion rod (17) is located inside the cavity (12); the end thereof is rotatably connected to a rotating wheel (19); and the push plate (10) and the insertion rod (17) are movably connected via the rotating wheel (19).
8. The cell counting chamber integrating sample loading, staining and counting according to claim 7, characterized in that: The support block (6) has an anti-slip groove (25) on its surface.