Virus transduction kit

Abstract

The utility model discloses a virus transduction kit, which relates to the technical field of biotechnology and comprises a centrifugal pipeline system, a sample liquid pipeline branch system, a virus liquid pipeline branch system, a pre-transduction replacement liquid pipeline branch system, a post-transduction replacement liquid pipeline branch system, a product liquid pipeline branch system and a waste liquid pipeline branch system. Wherein the sample liquid pipeline branch system, the virus liquid pipeline branch system, the pre-transduction replacement liquid pipeline branch system, the post-transduction replacement liquid pipeline branch system, the product liquid pipeline branch system and the waste liquid pipeline branch system are all connected with the centrifugal pipeline system. All operations of concentration / washing / transduction of the cell sap can be completed in a closed environment at a time, the operation is simple, the influence of human factors is small, the solution pollution is small, and the quality of cell virus transduction is ensured.

Description

Technical Field

[0001] This application relates to the field of biotechnology, and particularly to a virus transduction kit. Background Art

[0002] Transduction refers to the introduction of foreign genes into eukaryotic or prokaryotic cells through recombinant viral vectors, thereby causing gene recombination.

[0003] In the prior art, cells to be transduced are concentrated or washed manually in a Class A clean environment, and then the virus is added to the cells and virus transduction is carried out in a centrifuge.

[0004] In this process, the operator needs to manually perform operations such as loading cells into tubes, pipetting, sealing the tube mouths, and centrifuging repeatedly; and because centrifuges are generally in a Class B or Class C environment, the centrifuge tubes containing cells must be manually sealed reliably during centrifugation.

[0005] The above-mentioned sub-packaging process is cumbersome and redundant, and the manual operation is also affected by different proficiency levels, resulting in differences in results; moreover, the manual operation method has an open environment and is prone to contamination. Therefore, there is an urgent need for a device that can achieve virus transduction under closed conditions. Utility Model Content

[0006] The main purpose of this application is to provide a virus transduction kit, aiming to solve the problems that the process of traditional manual operation of cell transduction instruments in the prior art is cumbersome and the transduction process is prone to contamination due to manual operation.

[0007] The technical solution adopted in this application is as follows:

[0008] A virus transduction kit, comprising:

[0009] A centrifugation pipeline system, which is used for centrifugal stratification treatment of the liquid entering the pipeline system;

[0010] A sample liquid pipeline branch system, which is connected to the centrifugation pipeline system and is used to introduce the sample liquid into the centrifugation pipeline system;

[0011] A virus liquid pipeline branch system, which is connected to the centrifugation pipeline system and is used to introduce the virus liquid into the centrifugation pipeline system;

[0012] A pre-transduction replacement liquid pipeline branch system, which is connected to the centrifugation pipeline system and is used to introduce the pre-transduction replacement liquid into the centrifugation pipeline system;

[0013] A post-transduction replacement liquid pipeline branch system, which is connected to the centrifugation pipeline system and is used to introduce the post-transduction replacement liquid into the centrifugation pipeline system;

[0014] A product liquid pipeline branch system, which is connected to the centrifugal pipeline system to receive the sample liquid formed after centrifugation by the centrifugal pipeline system; and,

[0015] A waste liquid pipeline branch system, which is connected to the centrifugal pipeline system to receive the waste liquid formed after centrifugal separation by the centrifugal pipeline system.

[0016] Optionally, the centrifugal pipeline system includes:

[0017] A main centrifugal pipeline hose, which is respectively connected to a branch main hose A and a branch main hose B through a three-way A. The branch main hose A is connected to the sample liquid pipeline branch system, the pre-transduction replacement liquid pipeline branch system, the virus liquid pipeline branch system, and the waste liquid pipeline branch system through a five-way. The branch main hose B is connected to the post-transduction replacement liquid pipeline branch system and the product liquid pipeline branch system through a three-way B; and,

[0018] A centrifuge cup, which is connected to the main centrifugal pipeline hose.

[0019] Optionally, the main centrifugal pipeline hose is connected to a pressure detection branch hose through a three-way C, and an air filter is provided on the pressure detection branch hose.

[0020] Optionally, the sample liquid pipeline branch system includes a sample liquid pipeline branch hose. The head end of the sample liquid pipeline branch hose is connected to a puncture device A through a Luer connector, and a Robert clip A is provided on the sample liquid pipeline branch hose.

[0021] Optionally, the virus liquid pipeline branch system includes a virus liquid pipeline branch hose. The head end of the virus liquid pipeline branch hose is provided with a virus liquid bag, and a Robert clip B is provided on the virus liquid pipeline branch hose.

[0022] Optionally, the pre-transduction replacement liquid pipeline branch system includes a pre-transduction replacement liquid pipeline branch hose. The head end of the pre-transduction replacement liquid pipeline branch hose is connected to a puncture device C through a Luer connector, and a Robert clip C is provided on the pre-transduction replacement liquid pipeline branch hose.

[0023] Optionally, the post-transduction replacement liquid pipeline branch system includes a post-transduction replacement liquid pipeline branch hose. The head end of the post-transduction replacement liquid pipeline branch hose is connected to a puncture device D through a Luer connector, and a Robert clip D is provided on the post-transduction replacement liquid pipeline branch hose.

[0024] Optionally, the product liquid pipeline branch system includes a product liquid pipeline branch hose, a product bag is arranged at the head end of the product liquid pipeline branch hose, and a Robert clip E is arranged on the product liquid pipeline branch hose.

[0025] Optionally, the product liquid pipeline branch hose is connected to a sampling branch hose through a tee D, and a sampling pipe and a Robert clip F are arranged on the sampling branch hose.

[0026] Optionally, the waste liquid pipeline branch system includes a waste liquid pipeline branch hose, a waste liquid bag is connected to the head end of the waste liquid pipeline branch hose, and a Robert clip G is arranged on the waste liquid pipeline branch hose.

[0027] Compared with the prior art, the beneficial effects of the present application are:

[0028] A virus transduction kit provided by an embodiment of the present application, by setting a centrifugation pipeline system, a sample liquid pipeline branch system, a virus liquid pipeline branch system, a pre-transduction replacement liquid pipeline branch system, a post-transduction replacement liquid pipeline branch system, a product liquid pipeline branch system, and a waste liquid pipeline branch system, connecting the sample liquid pipeline branch system, the virus liquid pipeline branch system, the pre-transduction replacement liquid pipeline branch system, the post-transduction replacement liquid pipeline branch system, the product liquid pipeline branch system, and the waste liquid pipeline branch system to the centrifugation pipeline system, can complete all operations of concentrating / washing / transducing cell liquid in a closed environment at one time, with simple operation, less affected by human factors, less solution pollution, and ensuring the quality of cell virus transduction. Description of the Drawings

[0029] Figure 1 It is a schematic structural diagram of the virus transduction kit provided by an embodiment of the present application from one perspective.

[0030] Explanation of the reference numerals in the drawings:

[0031] 1 - Puncture device C, 2 - Protective sheath for puncture device C, 3 - Male Luer connector, 4 - Robert clip C, 5 - Branch hose for pre - transduction replacement fluid pipeline, 6 - Puncture device A, 7 - Protective sheath for puncture device A, 8 - Male Luer connector A, 9 - Robert clip A, 10 - Branch hose for sample fluid pipeline, 11 - Five - way joint, 12 - Main branch hose A, 13 - Three - way joint A, 14 - Main centrifugation pipeline hose A, 15 - Three - way joint C, 16 - Main centrifugation pipeline hose B, 17 - Centrifugation cup, 18 - Pressure detection branch hose, 19 - Air filter, 20 - Main branch hose B, 21 - Three - way joint B, 22 - Branch hose for post - transduction replacement fluid pipeline A, 23 - Robert clip D, 24 - Branch hose for post - transduction replacement fluid pipeline B, 25 - Male Luer connector, 26 - Puncture device D, 27 - Protective sheath for puncture device D, 28 - Branch hose for product fluid pipeline A, 29 - Three - way joint D, 30 - Branch hose for product fluid pipeline B, 31 - Robert clip E, 32 - Product bag, 33 - Sampling branch hose, 34 - Robert clip F, 35 - Three - way joint E, 36 - Sampling tube, 37 - Air filter, 38 - Female Luer connector cap, 39 - Branch hose, 40 - Female Luer connector, 41 - Female Luer connector cap, 42 - Branch hose for waste liquid pipeline, 43 - Robert clip G, 44 - Waste liquid bag, 45 - Branch hose for virus liquid pipeline, 46 - Robert clip B, 47 - Virus liquid bag, 48 - Upper pipeline chuck, 49 - Lower pipeline chuck, 50 - Pipeline label. Detailed implementation mode

[0032] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the scope of protection of the present application.

[0033] It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present application are only used to explain the relative position relationship and movement conditions between components in a specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0034] In the present application, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the internal communication of two components or the interaction relationship between two components, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0035] In addition, if the embodiments of the present application involve descriptions such as "first", "second", etc., the descriptions of "first", "second", etc. are only for descriptive purposes and should not be construed as indicating or implying their relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and / or" appearing throughout the text includes three parallel scenarios. Taking "A and / or B" as an example, it includes Scenario A, or Scenario B, or the scenario where both A and B are satisfied simultaneously. In addition, the technical solutions between the various embodiments may be combined with each other, but it must be based on the ability of those of ordinary skill in the art to implement. When the combination of technical solutions results in contradictions or is unable to be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present application.

[0036] Referring to the attached Figure 1 , the embodiments of the present application provide a virus transduction kit, which includes a centrifugation pipeline system, a sample liquid pipeline branch system, a virus liquid pipeline branch system, a pre-transduction replacement liquid pipeline branch system, a post-transduction replacement liquid pipeline branch system, a product liquid pipeline branch system, and a waste liquid pipeline branch system. Among them, the sample liquid pipeline branch system, the virus liquid pipeline branch system, the pre-transduction replacement liquid pipeline branch system, the post-transduction replacement liquid pipeline branch system, the product liquid pipeline branch system, and the waste liquid pipeline branch system are all connected to the centrifugation pipeline system.

[0037] Specifically:

[0038] See Figure 1As shown, the centrifugal pipeline system includes a main centrifugal pipeline hose and a centrifuge cup 17. The centrifuge cup 17 is specifically a piston-type centrifuge cup. The piston can move up and down to extrude or suck in liquid, cooperate with the equipment to rotate at high speed to generate centrifugal force, realize the gradient centrifugation and layering of the liquid in the centrifuge cup, or rotate at high speed to generate centrifugal force to deform cells, make part of the cell membrane thinner, and the virus enters the cell under the action of force to achieve virus transduction. The centrifuge cup 17 is connected to the head end of the main centrifugal pipeline hose. The main centrifugal pipeline hose is respectively connected to a branch main hose A12 and a branch main hose B20 through a tee A13. The branch main hose A12 is connected to a sample liquid pipeline branch system, a pre-transduction replacement liquid pipeline branch system, a virus liquid pipeline branch system, and a waste liquid pipeline branch system through a five-way 11. The branch main hose B20 is connected to a post-transduction replacement liquid pipeline branch system and a product liquid pipeline branch system through a tee B21. At the same time, the main centrifugal pipeline hose is connected to a pressure detection branch hose 18 through a tee C15. An air filter 19 is provided on the pressure detection branch hose 18. The air filter 19 filters out bacteria in the air to achieve a sterile effect for the filtered gas. The pressure measurement sensing device is connected to the air inlet end of the air filter and connected to the pressure detection branch hose, so as to detect the pressure of the kit pipeline and ensure the normal progress of the process. The tee C15 divides the main centrifugal pipeline hose into two ends, namely a main centrifugal pipeline hose A14 and a main centrifugal pipeline hose B16.

[0039] The sample liquid pipeline branch system includes a sample liquid pipeline branch hose 10. The head end of the sample liquid pipeline branch hose 10 is equipped with a male Luer connector A8. The puncture device A6 is equipped with a female Luer connector. The puncture device A6 is connected to the sample liquid pipeline branch hose 10 integrally through the cooperation of the female Luer connector and the male Luer connector A8. The puncture device A6 can be directly inserted and connected to the butterfly puncture port of the process bag. The puncture device A6 is sleeved with a puncture device A protective sleeve 7. The puncture protective sleeve is used to prevent the insertion part of the puncture device A6 from being contaminated and prevent the sharp part of the puncture device from piercing the sterile barrier or hurting people. A Robert clip A9 is provided on the sample liquid pipeline branch hose 10. Currently, opening or closing the Robert clip can achieve non-compression or compression of the hose. When compressed, the hose is blocked and the liquid cannot pass through; the hose passes through the middle hole of the Robert clip, and there is a slight interference so that the Robert clip will not slide arbitrarily.

[0040] The virus liquid pipeline branch system includes a virus liquid pipeline branch hose 45. A virus liquid bag 47 is provided at the head end of the virus liquid pipeline branch hose 45. A Robert clip B46 is provided on the virus liquid pipeline branch hose 45.

[0041] The pre-transduction replacement fluid pipeline branch system includes a pre-transduction replacement fluid pipeline branch hose 5. The head end of the pre-transduction replacement fluid pipeline branch hose 5 is equipped with a male Luer connector 3. The puncture device C1 is self-equipped with a female Luer connector, and the puncture device C1 is connected to the pre-transduction replacement fluid pipeline branch hose 5 as a whole through the cooperation of the male Luer connector and the female Luer connector. A puncture device C protective sleeve 2 is sleeved on the puncture device C1, and a Robert clamp C4 is provided on the pre-transduction replacement fluid pipeline branch hose 5.

[0042] The post-transduction replacement fluid pipeline branch system has the same structure as the pre-transduction replacement fluid pipeline branch system, including a post-transduction replacement fluid pipeline branch hose. The head end of the post-transduction replacement fluid pipeline branch hose is equipped with a male Luer connector 25. The puncture device D26 is self-equipped with a female Luer connector, and the puncture device D26 is connected to the post-transduction replacement fluid pipeline branch hose as a whole through the cooperation of the male Luer connector and the female Luer connector. A puncture device D protective sleeve 27 is sleeved on the puncture device D26, and a Robert clamp D23 is provided on the post-transduction replacement fluid pipeline branch hose. The Robert clamp D23 divides the post-transduction replacement fluid pipeline branch hose into a post-transduction replacement fluid pipeline branch hose A22 and a post-transduction replacement fluid pipeline branch hose B24.

[0043] The product fluid pipeline branch system includes a product fluid pipeline branch hose. The head end of the product fluid pipeline branch hose is provided with a product bag 32, and a Robert clamp E31 is provided on the product fluid pipeline branch hose. The product fluid pipeline branch hose is connected with a sampling branch hose 33 through a three-way joint D29. The three-way joint D29 divides the product fluid pipeline branch hose into a product fluid pipeline branch hose A28 and a product fluid pipeline branch hose B30. A sampling tube 36 and a Robert clamp F34 are provided on the sampling branch hose 33. The sampling tube 36 sucks in process fluid through squeezing to generate negative pressure. The head end of the sampling tube is connected with an air filter 37. A female Luer connector is installed at the air inlet of the air filter 37 and is connected in a Luer form with a male Luer to sample the process fluid in the sampling tube. The female Luer connector is blocked by a female Luer connector cap 38 to prevent liquid from flowing out when not connected to other pipelines. Of course, the sampling branch hose is connected with a branch hose 39 through a three-way joint E35. The branch hose 39 is connected with a female Luer connector 40 for connection with an external pipeline. Similarly, a female Luer connector cap 41 is sleeved on the female Luer connector 40 to prevent liquid from flowing out when not connected to other pipelines.

[0044] The waste liquid pipeline branch system includes a waste liquid pipeline branch hose 42. The head end of the waste liquid pipeline branch hose 42 is connected with a waste liquid bag 44, and a Robert clamp G43 is provided on the waste liquid pipeline branch hose 42.

[0045] In the above, for the convenience of distinguishing each pipeline system, pipeline labels 50 are provided on the corresponding pipeline hoses as the identification of the pipeline to indicate the process liquid of the pipeline.

[0046] In addition, a pipeline chuck is provided on this kit. The pipeline chuck includes an upper pipeline chuck 48 and a lower pipeline chuck 49. The positions of the pipelines of the kit are fixed by the cooperation of the upper pipeline chuck 48 and the lower pipeline chuck 49 to prevent the pipelines of the kit from being scattered; each pipeline of the sleeve is clamped and fixed with the pipeline chuck.

[0047] The following specifically introduces the pipeline connection relationships among the centrifugal pipeline system, the sample liquid pipeline branch system, the virus liquid pipeline branch system, the pre-transduction replacement liquid pipeline branch system, the post-transduction replacement liquid pipeline branch system, the product liquid pipeline branch system, and the waste liquid pipeline branch system.

[0048] See Figure 1 As shown, between the pre-transduction replacement liquid pipeline branch system and the centrifugal pipeline system:

[0049] Pre-transduction replacement liquid pipeline branch hose 5 - five-way joint 11 - branch main hose A12 - three-way joint A13 - centrifugal pipeline main hose A14 - three-way joint C15 - centrifugal pipeline main hose B16 - centrifuge cup 17.

[0050] Between the sample liquid pipeline branch system and the centrifugal pipeline system:

[0051] Sample liquid pipeline branch hose 10 - five-way joint 11 - branch main hose A12 - three-way joint A13 - centrifugal pipeline main hose A14 - three-way joint C15 - centrifugal pipeline main hose B16 - centrifuge cup 17.

[0052] Between the virus liquid pipeline branch system and the centrifugal pipeline system:

[0053] Virus liquid pipeline branch hose 45 - five-way joint 11 - branch main hose A12 - three-way joint A13 - centrifugal pipeline main hose A14 - three-way joint C15 - centrifugal pipeline main hose B16 - centrifuge cup 17.

[0054] Between the waste liquid pipeline branch system and the centrifugal pipeline system:

[0055] Waste liquid pipeline branch hose 42 - five-way joint 11 - branch main hose A12 - three-way joint A13 - centrifugal pipeline main hose A14 - three-way joint C15 - centrifugal pipeline main hose B16 - centrifuge cup 17.

[0056] Between the post-transduction replacement liquid pipeline branch system and the centrifugal pipeline system:

[0057] Post-transduction replacement liquid pipeline branch hose B24 - post-transduction replacement liquid pipeline branch hose A22 - three-way joint B21 - branch main hose B20 - three-way joint A13 - centrifugal pipeline main hose A14 - three-way joint C15 - centrifugal pipeline main hose B16 - centrifuge cup 17.

[0058] Between the product liquid pipeline branch system and the centrifugal pipeline system:

[0059] Product liquid pipeline branch hose B30 -- three-way joint D29 -- product liquid pipeline branch hose A28 -- three-way joint B21 -- branch main hose B20 -- three-way joint A13 -- centrifugal pipeline main hose A14 -- three-way joint C15 -- centrifugal pipeline main hose B16 -- centrifuge cup 17.

[0060] Sampling branch hose 33 -- three-way joint D29 -- product liquid pipeline branch hose A28 -- three-way joint B21 -- branch main hose B20 -- three-way joint A13 -- centrifugal pipeline main hose A14 -- three-way joint C15 -- centrifugal pipeline main hose B16 -- centrifuge cup 17.

[0061] Based on the above structure, the virus transduction kit provided by the embodiment of the present application can perform processes of concentrating, pre-washing, transduction, and post-washing on the sample liquid.

[0062] Among them, the specific process of concentration is as follows:

[0063] Connect the puncture device A6 of the sample liquid pipeline branch hose 10 to the butterfly puncture port of the sample bag; at this time, the control device generates negative pressure, and the internal piston of the centrifuge cup 17 moves downward, generating negative pressure on the connected pipeline; the sample liquid will pass through the sample bag, puncture device A6, male Luer connector A8, sample liquid pipeline branch hose 10 (open the Robert clip A9), five-way joint 11, branch main hose A12, three-way joint A13, centrifugal pipeline main hose A14, three-way joint C15, centrifugal pipeline main hose B16 and flow into the centrifuge cup 17; then the centrifuge cup 17 rotates at a high speed to generate centrifugal force, and the sample liquid will be gradient-layered due to different masses; the waste liquid is pushed by the upward movement of the piston of the centrifuge cup 17 and enters the waste liquid bag 44 through the centrifugal pipeline main hose B16, three-way joint C15, centrifugal pipeline main hose A14, three-way joint A13, branch main hose A12, five-way joint 11, waste liquid pipeline branch hose 42 (open the Robert clip G43), and the remaining concentrated (higher density) cells are left in the centrifuge cup 17.

[0064] The specific process of pre-washing is as follows:

[0065] Connect the puncture device C1 at the front end of the pre-transduction replacement liquid pipeline branch hose 5 to the butterfly puncture port of the pre-transduction replacement bag; the pre-transduction replacement liquid will pass through the puncture device C1, male Luer connector 3, pre-transduction replacement liquid pipeline branch hose 5 (open the Robert clip C4), five-way joint 11, branch main hose A12, three-way joint A13, centrifugal pipeline main hose A14, three-way joint C15, centrifugal pipeline main hose B16 and flow into the centrifuge cup 17;

[0066] Then, the sample solution is introduced into the centrifuge cup 17 through the sample bag, puncture device A6, male Luer connector 8, sample solution pipeline branch hose 10 (open the Robert clip A9), five-way valve 11, branch main hose A12, three-way valve A13, centrifuge pipeline main hose A14, three-way valve C15, and centrifuge pipeline main hose B16; by rotating the centrifuge cup, the pre-replacement washing solution and the sample solution are mixed, and then re-stratified by gradient to achieve the washing of the sample; then, the waste liquid after washing is pushed by the upward movement of the piston of the centrifuge cup 17 through the centrifuge pipeline main hose B16, three-way valve C15, centrifuge pipeline main hose A14, three-way valve A13, branch main hose A12, five-way valve 11, waste liquid pipeline branch hose 42 (open the Robert clip G43) and into the waste liquid bag 44, and the clean cells remain in the centrifuge cup 17.

[0067] The specific process of transduction is as follows:

[0068] After the above-mentioned pre-washing is completed, at this time, the virus is introduced into the centrifuge cup 17 through the virus bag 47, virus solution pipeline branch hose 45 (open the Robert clip B46), five-way valve 11, branch main hose A12, three-way valve A13, centrifuge pipeline main hose A14, three-way valve C15, and centrifuge pipeline main hose B16; the centrifuge cup 17 rotates at a high speed again, the centrifugal force deforms the cells, part of the cell membrane becomes thinner, and the virus enters the cells under the action of the force to achieve virus transduction;

[0069] Then, the piston of the centrifuge cup 17 moves upward and enters the waste liquid bag 44 through the centrifuge pipeline main hose B16, three-way valve C15, centrifuge pipeline main hose A14, three-way valve A13, branch main hose A12, five-way valve 11, waste liquid pipeline branch hose 42 (open the Robert clip G43), and the transduced cells remain in the centrifuge cup 17.

[0070] The specific process of post-washing is as follows:

[0071] When the transduction is completed according to the transduction process, the cells are then in the separation cup. Then, the post-transduction liquid will flow into the centrifuge cup 17 through the puncture device D26, male Luer connector 25, post-transduction replacement liquid pipeline branch hose B24 (open the Robert clamp D23), post-transduction replacement liquid pipeline branch hose A22, three-way joint B21, branch main hose B20, three-way joint A13, centrifuge pipeline main hose A14, three-way joint C15, and centrifuge pipeline main hose B16; through the rotation of the centrifuge cup 17, the post-replacement washing liquid and the post-transduction sample liquid are mixed, and then re-stratified by gradient to achieve the washing of the post-transduction sample; then, the piston of the separation cup 17 moves upward to push the washed waste liquid through the centrifuge pipeline main hose B16, three-way joint C15, centrifuge pipeline main hose A14, three-way joint A13, branch main hose A12, five-way joint 11, and waste liquid pipeline branch hose 42 (open the Robert clamp G43) into the waste liquid bag 44; the remaining clean post-transduction cells in the separation cup 17, and the sample liquid after removing the waste liquid passes through the centrifuge pipeline main hose B16, three-way joint C15, centrifuge pipeline main hose A14, three-way joint A13, branch main hose B20, three-way joint B21, product liquid pipeline branch hose A28, three-way joint D29, and product liquid pipeline branch hose B30 (open the Robert clamp E31) into the product bag 32.

[0072] In summary, a virus transduction kit proposed in an embodiment of the present application, by setting up a centrifuge pipeline system, a sample liquid pipeline branch system, a virus liquid pipeline branch system, a pre-transduction replacement liquid pipeline branch system, a post-transduction replacement liquid pipeline branch system, a product liquid pipeline branch system, and a waste liquid pipeline branch system, connecting the sample liquid pipeline branch system, the virus liquid pipeline branch system, the pre-transduction replacement liquid pipeline branch system, the post-transduction replacement liquid pipeline branch system, the product liquid pipeline branch system, and the waste liquid pipeline branch system with the centrifuge pipeline system, can achieve the concentration / washing / transduction of cell liquid in a closed environment at one time, with simple operation, less influence by human factors, less solution pollution, and ensuring the quality of cell virus transduction.

[0073] The above are only the preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A viral transduction kit, characterized in that: include: A centrifugal piping system, wherein the centrifugal piping system is used to perform centrifugal stratification treatment on the liquid entering the piping system; A sample liquid pipeline branch system, the sample liquid pipeline branch system is connected to the centrifugal pipeline system, and is used to introduce the sample liquid into the centrifugal pipeline system; a virus liquid pipeline branch system, wherein the virus liquid pipeline branch system is connected to the centrifugal pipeline system and is used to introduce the virus liquid into the centrifugal pipeline system; a pre-transduction replacement fluid pipeline branch system, wherein the pre-transduction replacement fluid pipeline branch system is connected to the centrifugal pipeline system to introduce the pre-transduction replacement fluid into the centrifugal pipeline system; a post-transduction replacement fluid pipeline branch system, wherein the post-transduction replacement fluid pipeline branch system is connected to the centrifugal pipeline system to introduce the post-transduction replacement fluid into the centrifugal pipeline system; A product liquid pipeline branch system, the product liquid pipeline branch system is connected to the centrifugal pipeline system and is used to receive the sample liquid formed after centrifugation of the centrifugal pipeline system; as well as, A waste liquid pipeline branch system is connected to the centrifugal pipeline system to receive waste liquid formed after centrifugal separation by the centrifugal pipeline system.

2. The viral transduction kit according to claim 1, characterized in that The centrifuge piping system comprises: A centrifuge pipeline main hose, wherein the centrifuge pipeline main hose is respectively connected to a branch main hose A and a branch main hose B through a tee A, the branch main hose A is connected to the sample liquid pipeline branch system, the pre-transduction replacement liquid pipeline branch system, the virus liquid pipeline branch system and the waste liquid pipeline branch system through a five-way connection, and the branch main hose B is connected to the post-transduction replacement liquid pipeline branch system and the product liquid pipeline branch system through a tee B; and A centrifuge cup is connected to the centrifuge pipeline main hose.

3. The viral transduction kit according to claim 2, characterized in that The centrifugal pipeline main hose is connected to a pressure detection branch hose through a tee C, and an air filter is arranged on the pressure detection branch hose.

4. The viral transduction kit according to claim 1, characterized in that The sample liquid pipeline branching system comprises a sample liquid pipeline branching hose, the head end of which is connected to a puncture device A via a Luer connector, and a Robert clamp A is arranged on the sample liquid pipeline branching hose.

5. The viral transduction kit according to claim 1, characterized in that The virus liquid pipeline branch system comprises a virus liquid pipeline branch hose, a virus liquid bag is arranged at the head end of the virus liquid pipeline branch hose, and a Robert clamp B is arranged on the virus liquid pipeline branch hose.

6. The viral transduction kit according to claim 1, characterized in that The pre-transduction replacement fluid pipeline branch system comprises a pre-transduction replacement fluid pipeline branch hose, the head end of which is connected to a puncture device C via a Luer connector, and the pre-transduction replacement fluid pipeline branch hose is provided with a Robert clamp C.

7. The viral transduction kit according to claim 1, characterized in that The post-transduction replacement fluid pipeline branch system comprises a post-transduction replacement fluid pipeline branch hose, the head end of which is connected to a puncture device D via a Luer connector, and a Robert clamp D is provided on the post-transduction replacement fluid pipeline branch hose.

8. The viral transduction kit according to claim 1, characterized in that The product liquid pipeline branch system comprises a product liquid pipeline branch hose, a product bag is arranged at the head end of the product liquid pipeline branch hose, and a Robert clamp E is arranged on the product liquid pipeline branch hose.

9. The viral transduction kit according to claim 8, characterized in that The product liquid pipeline branch hose is connected to a sampling branch hose through a tee D, and a sampling tube and a Robert clamp F are provided on the sampling branch hose.

10. The viral transduction kit according to claim 1, characterized in that The waste liquid pipeline branch system comprises a waste liquid pipeline branch hose, the head end of the waste liquid pipeline branch hose is connected to a waste liquid bag, and a Robert clamp G is arranged on the waste liquid pipeline branch hose.

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