Reaction tube cover and reaction tube
By designing a reaction tube cover with pre-grooved grooves, the existing centrifuge tubes are solved for complex operation during sampling and the samples are easily contaminated, and the rapid sterile sample sampling and sealing are achieved, which is suitable for automated devices.
Patent Information
- Application Number
- CN202421638875.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-11
- Publication Date
- 2025-06-03
- Estimated Expiration
- 2034-07-11
AI Technical Summary
The existing centrifuge tube with breathable cover needs to be unscrewed when sampling is required. It is complex in operation and cannot be quickly sterile. In addition, the sample is easily contaminated, which limits its wide application in automation devices.
A reaction tube cover is designed, including a cover body, an elastic plug and a breathable membrane. A through hole and a plurality of breathable holes are opened on the cover body. The breathable membrane covers the breathable holes. The elastic plug is located in the through hole and sealed with the cover body, and a pre-cut groove is opened on the elastic plug. Samples can be added by passing through the pre-cut groove without unscrewing the tube cover.
It is possible to quickly and sterilely sample sampling without unscrewing the tube cover, avoid sample contamination, improve work efficiency, and maintain sealing at the pre-cut grooves.
Smart Images
Figure CN222930846U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the field of biotechnology, and particularly to a reaction tube cap and a reaction tube. Background Art
[0002] For existing centrifuge tubes with breathable caps, breathable holes are provided on the top surface of the tube cap, and a polytetrafluoroethylene (PTFE) film is welded below the breathable holes by a hot melt process. This film has the properties of breathable and bacteria-proof, and is impermeable to water, which can ensure the sealing of the tube. Therefore, it can meet both the centrifugation function and the culture function, integrating the centrifugation function and the culture function.
[0003] If the above centrifuge tube needs to add or take samples, the screw cap needs to be unscrewed first. The operation process is complex, and it is impossible to quickly add or take samples aseptically. The sample is easily contaminated and cannot be widely used in automated devices. Summary of the Utility Model
[0004] The purpose of the present application is to provide a reaction tube cap and a reaction tube, aiming to solve the technical problem in the prior art that the test tube cap needs to be removed to add or take samples.
[0005] To achieve the above purpose, a reaction tube cap adopted by the present application includes a cap body, an elastic plug, and a breathable membrane. A through hole and a plurality of breathable holes are provided on the cap body. The breathable holes are arranged around the through hole. The breathable membrane is connected to the end face of the cap body close to the tube body and covers the breathable holes. The elastic plug is located in the through hole and is hermetically connected to the cap body, and a pre-cut groove is provided on the elastic plug.
[0006] As a preferred technical solution, the pre-cut groove is in a cross shape.
[0007] As a preferred technical solution, the elastic plug includes a body, a first boss, and a second boss. The first boss and the second boss are respectively arranged along the outer periphery of both ends of the body. The outer diameter of the second boss is greater than the outer diameter of the first boss, and both are greater than the diameter of the through hole. An annular clamping groove is defined between the first boss and the second boss, and the annular clamping groove is engaged with the through hole; the pre-cut groove is provided on the body.
[0008] As a preferred technical solution, hollowed-out portions are provided in the middle regions of both end faces of the body.
[0009] As a preferred technical solution, the elastic plug is a silica gel plug.
[0010] As a preferred technical solution, the end face of the cover body close to the pipe body is provided with a first convex part and a second convex part, both of which are annular. The first convex part is located between the through hole and the air-permeable hole, the second convex part is located between the air-permeable hole and the peripheral side of the cover body, and the air-permeable membrane is located between the first convex part and the second convex part.
[0011] As a preferred technical solution, the air-permeable membrane is thermally pressed and connected to the cover body, and both ends of the air-permeable membrane are thermally pressed to the first convex part and the second convex part respectively.
[0012] As a preferred technical solution, the outer side of the cover body has a plurality of anti-slip grooves, and the plurality of anti-slip grooves are uniformly arranged on the peripheral side of the cover body.
[0013] As a preferred technical solution, a process groove is provided on the end face of the cover body far from the air-permeable hole, and the process groove is arranged along the edge of the cover body.
[0014] The reaction tube cover in this application includes a pipe body and a cover. A through hole is provided on the cover body. An elastic plug is located in the through hole and is hermetically connected to the cover body, and a pre-cut groove is provided on the elastic plug. This application also provides a reaction tube, including the above-mentioned reaction tube cover and the pipe body. One end of the pipe body has an opening, and the reaction tube cover is screwed to the pipe body. Based on this, when adding or sampling, the sampling needle can pass through the pre-cut groove on the cover body and then enter the reaction tube body for adding or sampling. There is no need to unscrew the cover, which can prevent the sample from being contaminated and improve work efficiency at the same time. In addition, due to its own elastic effect, the elastic plug can be hermetically connected to the cover body and keep the pre-cut groove in a normally closed and sealed state. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] The following further describes this application in detail with reference to the drawings and preferred embodiments. However, those skilled in the art will understand that these drawings are only drawn for the purpose of explaining the preferred embodiments and should not be used as a limitation on the scope of this application. In addition, unless otherwise specified, the drawings are only intended to conceptually represent the composition or structure of the described object and may include exaggerated displays, and the drawings are not necessarily drawn to scale.
[0016] Figure 1 It is a schematic structural diagram of the reaction tube of the present utility model;
[0017] Figure 2 It is a schematic structural diagram of the reaction tube cover of the present utility model;
[0018] Figure 3 It is a schematic structural diagram of the installation of the reaction tube cover of the present utility model;
[0019] Figure 4 It is a schematic structural diagram of the installation of the reaction tube cover of the present utility model;
[0020] Figure 5 It is a sectional view of the reaction tube body of the present utility model;
[0021] Figure 6 For Figure 5 It is a partial enlarged view at position A in
[0022] Wherein: 1. Tube cap; 11. Cap body; 111. Through hole; 112. Vent hole; 113. First convex part; 114. Second convex part; 115. Anti-slip groove; 116. Process groove; 12. Elastic plug; 121. Pre-cut groove; 122. Body; 123. First boss; 124. Second boss; 125. Hollow part; 13. Breathable membrane; 2. Tube body. Specific embodiments
[0023] The embodiments of the present utility model will be described in detail below. The examples of the embodiments are shown in the drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and are intended to explain the present utility model, and should not be construed as a limitation to the present utility model.
[0024] In the description of the present application, it should be understood that the orientation or positional relationship indicated by the terms "front", "rear", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present application. In addition, in the description of the present utility model, "a plurality of" means two or more unless otherwise specifically defined.
[0025] Please refer to Figures 1-6 , a reaction tube cap and a reaction tube provided by an embodiment of the present application, comprising a tube body 2 and a tube cap 1; one end of the tube body 2 has an opening; the tube cap 1 includes a cap body 11, an elastic plug 12 and a breathable membrane 13, the cap body 11 is screwed to the tube body 2, the cap body 11 is provided with a through hole 111 and a plurality of vent holes 112, the vent holes 112 are arranged around the through hole 111, the breathable membrane 13 is connected to the end face of the cap body 11 close to the tube body 2 and covers the vent holes 112, the elastic plug 12 is located in the through hole 111 and is hermetically connected to the cap body 11, and a pre-cut groove 121 is provided on the elastic plug 12.
[0026] It should be noted that the above-mentioned pre-cut groove 121 is formed by cutting the elastic plug 12 with a tool body, and this cutting action does not cause the removal of the material of the elastic plug 12 itself, so that the elastic plug 12 remains sealed at the pre-cut groove 121.
[0027] In this embodiment, the tube body 2 has a cylindrical shape, which facilitates the clamping of the reaction tube by an automated device for rotational operation. A ventilation hole 112 is provided on the tube cap 1, and the ventilation hole 112 is covered with a ventilation membrane 13, which can achieve air exchange inside and outside the tube body to meet the oxygen demand of the sample. When it is necessary to fill the sample into the tube body 2 or take out the sample from the tube body 2, only need to pass the sampling needle through the pre-cut groove 121 on the elastic plug 12, and the sampling needle can enter the tube body 2 for sampling and adding samples without unscrewing the tube cap 1, which can prevent the sample from being contaminated and improve work efficiency at the same time; and after the sampling and adding are completed, when the sampling needle is removed from the elastic plug 12, the elastic plug 12 can ensure the sealing performance at the pre-cut groove 121 again by its own elastic extrusion effect.
[0028] The above reaction tube is applied to the field of sample detection. When the sample is tested on the machine, there is no need to open the lid of the tube body 2, which will significantly shorten the sample detection turnover time for laboratories with a large number of samples, reduce the direct contact between the inspection personnel and the sample, and avoid sample contamination.
[0029] Furthermore, the pre-cut groove 121 is in a cross shape. The cross-shaped pre-cut groove can adapt to the taking of samples of various shapes. Larger samples can be taken through the smaller pre-cut groove 121, and its sealing performance can be ensured.
[0030] As an alternative solution, the pre-cut groove 121 can also be set in various different forms such as a "one" shape, a "C" shape, an "X" shape, a "*" shape, a "rice" shape, etc.
[0031] Furthermore, the elastic plug 12 includes a main body 122, a first boss 123 and a second boss 124. The first boss 123 and the second boss 124 are respectively arranged along the outer circumference of both ends of the main body 122. The outer diameter of the second boss 124 is greater than that of the first boss 123, and both are greater than the diameter of the through hole 111. An annular clamping groove is defined between the first boss 123 and the second boss 124, and the annular clamping groove is clamped with the through hole 111 in a matching manner; the pre-cut groove 121 is opened on the main body 122. The elastic plug 12 is clamped with the through hole 111 through the annular clamping groove, realizing reliable sealing and fixing between the elastic plug 12 and the cover body 11. When the puncture needle passes through the pre-cut groove 121 for sampling, the elastic plug 12 will not have adverse phenomena such as falling off or arching.
[0032] In this embodiment, hollow parts 125 are provided in the middle regions of both end faces of the elastic plug 12. The setting of the hollow parts 125 makes the thickness of the elastic plug 12 at the position where the pre-cut groove 121 is opened smaller. On the premise of maintaining the seal of the cover body 11, the resistance of the pre-cut groove 121 to the puncture needle during puncture and removal can be reduced, facilitating sampling and adding samples.
[0033] In some embodiments of the present application, the elastic plug 12 is preferably made of silicone. This material has good elasticity, effectively preventing puncture debris from blocking the needle; it has poor hydrophilicity and good sealing performance for liquid samples. When the elastic plug 12 is inserted into the through hole 111, due to the elasticity of the silicone, the pre-cut groove 121 is kept normally closed. The pre-cut groove 121 is in a sealed state both in the natural state and after the sampling needle adds the sample, ensuring that the sample is sterile throughout the process.
[0034] Furthermore, on the end face of the cover body 11 close to the tube body 2, there are a first convex portion 113 and a second convex portion 114. The first convex portion 113 is located between the through hole 111 and the air vent hole 112, and the second convex portion 114 is located between the air vent hole 112 and the peripheral side of the cover body 11. The air permeable membrane 13 is located between the first convex portion 113 and the second convex portion 114. The first convex portion 113 and the second convex portion 114 position the air permeable membrane 13, and the air permeable membrane 13 is connected to the cover body 11, preventing the air permeable membrane 13 from falling off and the sample from overflowing.
[0035] In this embodiment, both ends of the air permeable membrane 13 are thermally pressed against the first convex portion 113 and the second convex portion 114 respectively, realizing the sealed connection between the air permeable membrane 13 and the cover body 11. It can effectively block bacteria and complete gas exchange to meet the oxygen demand of the sample.
[0036] In this embodiment, a plurality of anti-slip grooves 115 are evenly arranged on the peripheral side of the cover body 11. The anti-slip grooves 115 can play an anti-slip role, facilitating manual operation or cooperation with an automated fixture.
[0037] Furthermore, a process groove 116 is provided at the bottom of the cover body 11. A plurality of the process grooves 116 are evenly arranged at the bottom of the cover body 11. During the process of manufacturing the reaction tube, when opening the mold, the positioning protrusions on the mold are inserted into the process grooves 116, which can keep the tube cover 1 stationary. The mold can be gently rotated to screw out the tube cover 1. The operation is simple and convenient for demolding, which is beneficial to processing and production.
[0038] In this embodiment, the material of the air permeable membrane 13 is preferably a polytetrafluoroethylene (PTFE) film. This material has strong corrosion resistance, effectively preventing the sample from overflowing from the tube body 2 and causing harm to the instrument and the staff; this material has strong wear resistance, and the air permeable membrane 13 is not easily damaged, capable of sealing the sample under various conditions.
[0039] In summary, for the reaction tube cap and reaction tube provided in this embodiment, when sampling or adding samples is required, the user uses a puncture needle to sequentially pass through the upper hollow portion, the pre-cut groove 121, and the lower hollow portion of the elastic plug 12, and extends the puncture needle into the tube body 2 for operation; after sampling or adding samples is completed, the puncture needle sequentially passes through the lower hollow portion, the pre-cut groove 121, and the lower hollow portion of the elastic plug 12, and the puncture needle is removed from the reaction tube. Before and after sampling or adding samples, the pre-cut groove 121 always remains closed to ensure that the sample is sterile throughout the process; the hollow portion 125 is provided to reduce the thickness of the pre-cut groove 121, effectively reducing the resistance of the pre-cut groove 121 to the puncture needle when extending into the tube body 2, and at the same time, it can avoid contamination caused by the sample contacting the tube cap 1; in addition, ventilation holes 112 are provided on the cover body 11, and the ventilation holes 112 are covered with a ventilation film 13 on the inner side of the cover body 11, so as to complete the gas exchange inside and outside the reaction tube and meet the oxygen demand of the sample. This reaction tube can also be used for cell culture.
[0040] This specification discloses the present application with reference to the accompanying drawings, and also enables those skilled in the art to implement the present application, including manufacturing and using any device or system, adopting appropriate materials, and using any combined method. The scope of the present application is defined by the claimed technical solution and includes other examples that occur to those skilled in the art. As long as such other examples include structural elements that are not different from the literal language of the claimed technical solution, or such other examples contain equivalent structural elements that have no substantial difference from the literal language of the claimed technical solution, then such other examples should be considered to be within the protection scope determined by the claimed technical solution of the present application.
Claims
1. A reaction tube cover, characterized in that: It includes a cover body, an elastic plug and a breathable membrane, the cover body is provided with a through hole and a plurality of breathable holes, the breathable holes are arranged around the through hole, the breathable membrane is connected to the inner end surface of the cover body and covers the breathable holes, the elastic plug is located in the through hole and is sealed with the cover body, and the elastic plug is provided with a pre-cut groove.
2. The reaction tube cover according to claim 1, characterized in that: The pre-cut groove is in a cross shape.
3. The reaction tube cover according to claim 1, characterized in that: The elastic plug comprises a body, a first boss and a second boss, the first boss and the second boss are respectively arranged along the outer periphery of both ends of the body, the outer diameter of the second boss is larger than the outer diameter of the first boss, and both are larger than the diameter of the through hole, an annular groove is defined between the first boss and the second boss, and the annular groove is engaged with the through hole; The pre-cut groove is formed on the body.
4. The reaction tube cover according to claim 3, characterized in that: The middle areas of both end surfaces of the main body are provided with hollow parts.
5. The reaction tube cover according to claim 1, characterized in that: The elastic plug is a silicone plug.
6. The reaction tube cover according to claim 1, characterized in that: The inner end surface of the cover body is provided with a first convex portion and a second convex portion, both of which are annular. The first convex portion is located between the through hole and the air vent, the second convex portion is located between the air vent and the peripheral side of the cover body, and the air permeable membrane is located between the first convex portion and the second convex portion.
7. The reaction tube cover according to claim 6, characterized in that: The breathable film is connected to the cover body by heat pressing, and both ends of the breathable film are respectively heat pressed with the first convex part and the second convex part.
8. The reaction tube cover according to claim 1, characterized in that: The outer side of the cover body is provided with a plurality of anti-skid grooves, and the plurality of anti-skid grooves are evenly arranged on the peripheral side of the cover body.
9. The reaction tube cover according to claim 1, characterized in that: A process groove is provided on the end surface of the cover body away from the air vent, and the process groove is arranged along the edge of the cover body.
10. A reaction tube, characterized in that: It comprises a reaction tube cover and a tube body as described in any one of claims 1 to 9, wherein one end of the tube body has an opening, and the reaction tube cover is screwed to the tube body.