Small bioreactor with simple structure

Abstract

A small bioreactor with a simple structure comprises a tank body, a tank cover, a first fastener, a second fastener and a support, the tank body is a cylindrical container with an upper opening, the upper opening is provided with an outer edge, and the tank cover is provided with an inoculation port, a sampling port, an air inlet and a temperature measuring port; the small bioreactor with the simple structure, provided by the utility model, is light and easy to use, and compared with a conventional fermentation tank, complicated operation software and various detection devices are not needed, so that the available volume of the bioreactor is increased, the operation is easier, and the requirement on a culture medium is low; compared with a shake flask, the device is provided with a complete stirring and ventilating device, and a better fermentation result can be obtained generally.

Description

Technical Field

[0001] The utility model relates to the field of fermentation devices, in particular to a small bioreactor with a simple structure. Background Art

[0002] Bioreactor refers to a reaction vessel used for fermentation or physiological and biochemical reactions of microorganisms or cells. Bioreactors are generally made of glass and stainless steel, with good sealing and can withstand steam sterilization; they have temperature control devices to stably control the medium temperature at the set temperature. They have stirring and ventilation devices to meet the oxygen needs of microorganisms or cells during the reaction process. Bioreactors can be widely used in food, medicine, cosmetics, chemical industry and other fields.

[0003] Small bioreactors mainly refer to bioreactors of 1L and below. In order to achieve process monitoring, existing small bioreactors are usually equipped with cumbersome operating software and various detection devices. Various sensor devices that exist to achieve process monitoring reduce the available volume of small fermenters and increase the complexity of the operation process. The existence of complex operating software also increases the cumbersomeness of the operation process. This leads to the fact that compared with 5L bioreactors, small bioreactors on the market currently do not show obvious advantages. Complex pipeline connections, bulky tank structures, and cumbersome operating software have led to the low utilization rate of small fermenters in various fields. The development of simple, lightweight, and easy-to-use small bioreactors is the key to increasing the availability and practicality of small bioreactors. Utility Model Content

[0004] In order to solve the above problems, the utility model provides a small bioreactor with a simple structure, including a tank body, a tank cover, a first fastener, a second fastener and a bracket, the tank body is a cylindrical container with an upper opening, and the upper opening has an outer edge; the tank cover is provided with an inoculation port, a sampling port, an air inlet and a temperature measuring port, a motor is provided at the center of the tank cover, a stirring assembly is connected to the motor, a sealing ring is provided at the connection between the tank cover and the tank body, a baffle is provided inside the tank body, and a protrusion is also provided on the side of the tank cover, the inner diameter of the first fastener is smaller than the outer diameter of the protrusion, and the inner diameter of the second fastener is smaller than the outer diameter of the outer edge of the upper opening of the tank body.

[0005] Preferably, the bracket comprises a base, a supporting portion, a gripping portion and a supporting portion, the base and the supporting portion are connected via the supporting portion, and the gripping portion is provided on the supporting portion.

[0006] Preferably, the tank body is made of glass.

[0007] Preferably, the volume of the tank is 250-2000 ml.

[0008] Preferably, the first fastener is made of metal.

[0009] Preferably, the second fastener is made of silicone.

[0010] Preferably, the temperature measuring port is connected to a temperature control device, and the temperature control device includes a temperature measuring electrode, a controller and a heating blanket.

[0011] Preferably, the tank cover is also provided with a plurality of reserved openings communicating with the tank body.

[0012] The beneficial effects of the utility model are:

[0013] The utility model provides a small bioreactor with a simple structure, which is light and easy to use. Compared with a conventional fermenter, it does not require complicated operating software and various detection devices, thereby increasing the available volume of the reactor, making it easier to operate and requiring less culture medium. Compared with a shake flask, it has a complete stirring and ventilation device, and can usually obtain better fermentation results. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0015] Figure 1 It is a schematic diagram of the structure of the implementation method of the utility model;

[0016] Figure 2 It is a side view of an embodiment of the utility model;

[0017] Figure 3 It is a schematic diagram of a tank cover and a baffle according to an embodiment of the utility model. DETAILED DESCRIPTION

[0018] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0019] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0020] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, the terms "installation", "connection", and "coupling" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or a connection that allows mutual communication; it can be a direct connection, or an indirect connection through an intermediate medium, and can be the internal communication of two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0021] The following disclosure provides many different embodiments or examples for implementing different structures of the present utility model. To simplify the disclosure of the present utility model, the components and settings of specific examples are described below. Of course, they are only examples and are not intended to limit the present utility model.

[0022] As Figures 1-3 shown, a small bioreactor with a simple structure according to an embodiment of the present utility model includes a tank body 1, a tank cover 2, a first fastener 4, a second fastener 5, and a bracket 3. The tank body 1 has a cylindrical shape with an open upper part, and the open upper part has an outer edge 22. The side of the tank cover 2 has a protruding part 21. The inner diameter of the first fastener 4 is smaller than the diameter of the protruding part 21, and the inner diameter of the second fastener 5 is smaller than the outer diameter of the outer edge 22 of the open upper part of the tank body 1. The first fastener 4 is installed on the upper part of the tank cover 2, and the second fastener 5 is installed from the bottom of the tank body 1. The tank body 1 and the tank cover 2 are fixed by tightening the first fastener 4 and the second fastener 5. A sealing ring is also provided on the tank cover 2 to avoid gaps at the connection between the tank cover 2 and the tank body 1, thereby improving the airtightness of the fermenter.

[0023] An inoculation port 23 is provided on the tank cover 2 for adding a solution into the tank body 1; a sampling port 24 is used for sampling operations from the fermenter, and an air inlet 25 is used to connect to an air inlet device, such as an air pump. In some embodiments, an air flow meter is connected to the air pump to control the air intake.

[0024] A temperature measurement port 26 is connected to a temperature control device. The temperature control device includes a temperature measurement electrode, a controller, and a heating blanket. The heating blanket usually wraps the lower part of the tank body 1. The temperature control electrode extends into the tank body 1 from the temperature measurement port 26 to detect and feedback the temperature of the solution in the fermenter, and then the working state of the heating blanket is adjusted through the controller to achieve temperature control.

[0025] A motor 7 is provided at the geometric center of the tank cover 2. A stirring assembly 8 is connected to the shaft of the motor 7. The stirring assembly 8 is rotated by the motor 7 to stir the solution in the fermenter.

[0026] The bracket 3 includes a base 31, a support portion 32, a gripping portion 33 and a supporting portion 34. The base 31 and the supporting portion 34 are connected by the support portion 32. The surface of the supporting portion 34 has an opening, and the inner diameter of the opening is smaller than the outer diameter of the second fastener 5. The tank body 1 is placed in through the opening, and the supporting portion 34 can support and fix the tank body 1 through the second fastener 5; the gripping portion 33 is arranged on the support portion 32 to facilitate the operator to grip and move it by hand.

[0027] In some embodiments, the tank body 1 is made of glass material, which is convenient for the operator to observe the state of the fermented matter in the tank; the volume of the tank body can be set to 250 ml - 2000 ml, and common specifications include 250 ml, 500 ml, 1000 ml, 1500 ml, and 2000 ml.

[0028] The first fastener 4 is made of metal material, and the second fastener 5 is made of silica gel material. In this way, there is a rigid and flexible material contact between the first fastener 4 and the tank cover 2, and there is a rigid and flexible material contact between the second fastener 5 and the tank body 1, thereby improving the airtightness of the fermenter and reducing the risk of damage.

[0029] A baffle 9 is arranged inside the tank body 1. The baffle 9 plays a blocking role on the solution during the stirring process to prevent the formation of eddy currents during the stirring process, resulting in uneven stirring effect. In this embodiment, a movable baffle 9 design can be adopted. As shown in the figure, one end of the four baffles 9 in the vertical direction is connected. When in use, it is placed in the tank body 1, and the baffle 9 abuts against the tank body 1; it can be taken out after the fermentation work is completed and cleaned and stored separately.

[0030] In some embodiments, the baffle 9 can also be uniformly fixedly installed on the tank body 1, and the present utility model does not limit this.

[0031] In some embodiments, in addition to the inoculation port 23, the sampling port 24, the air inlet port 25 and the temperature measuring port 26, a plurality of reserved ports 27 are also arranged on the tank cover 2. The reserved ports 27 are used to expand modules such as pH, dissolved oxygen, and defoaming.

[0032] Although the embodiments of the present utility model have been shown and described, those of ordinary skill in the art can understand that: various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and purposes of the present utility model, and the scope of the present utility model is defined by the claims and their equivalents.

Claims

1. A small bioreactor with a simple structure, characterized in that: It includes a tank body, a tank cover, a first fastener, a second fastener and a bracket. The tank body is a cylindrical container with an upper opening, and the upper opening has an outer edge. The tank cover is provided with an inoculation port, a sampling port, an air inlet and a temperature measuring port. A motor is provided at the center of the tank cover, and a stirring assembly is connected to the motor. A sealing ring is provided at the connection between the tank cover and the tank body, and a baffle is provided inside the tank body. A protrusion is also provided on the side of the tank cover, the inner diameter of the first fastener is smaller than the outer diameter of the protrusion, and the inner diameter of the second fastener is smaller than the outer diameter of the outer edge of the upper opening of the tank body.

2. A small bioreactor with a simple structure according to claim 1, characterized in that: The bracket comprises a base, a supporting portion, a gripping portion and a supporting portion. The base and the supporting portion are connected via the supporting portion, and the gripping portion is arranged on the supporting portion.

3. A small bioreactor with a simple structure according to claim 1, characterized in that: The tank body is made of glass.

4. A small bioreactor with a simple structure according to claim 1, characterized in that: The volume of the tank is 250-2000ml.

5. A small bioreactor with a simple structure according to claim 1, characterized in that: The first fastener is made of metal.

6. A small bioreactor with a simple structure according to claim 1, characterized in that: The second fastener is made of silicone.

7. A small bioreactor with a simple structure according to claim 1, characterized in that: The temperature measuring port is connected to a temperature control device, and the temperature control device includes a temperature measuring electrode, a controller and a heating blanket.

8. A small bioreactor with a simple structure according to claim 1, characterized in that: The tank cover is also provided with a plurality of reserved openings communicating with the tank body.

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