Stem cell sorting device for stem cell research

Abstract

The utility model relates to the technical field of stem cells, and discloses a stem cell sorting device for stem cell research, which comprises a bottom plate, four supporting legs are fixedly connected to the left side of the top of the bottom plate, a dispersing tank is fixedly connected to the tops of the four supporting legs, a motor is fixedly connected to the bottom of the dispersing tank, and a motor is fixedly connected to the output end of the motor. A plurality of stirring rods are fixedly connected to the periphery, a plurality of connecting rods are fixedly connected to the middles of the stirring rods, a fixing plate is fixedly connected to the ends, away from the stirring rods, of the connecting rods, metal rods are slidably connected to the two sides of the interior of the fixing plate, and first springs are arranged on the outer sides of the metal rods in a sleeving mode. According to the stem cell separation device, the stem cells in the dispersion tank are fully stirred, scattered and separated through the stirring rod, so that the separation efficiency and purity are improved, the stem cells adhered to the inner side wall of the dispersion tank are reduced under the action of the scraping plate, waste is reduced, and the practicability of the device is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cells, in particular to a stem cell sorting device for stem cell research. Background Art

[0002] Stem cells are a class of cells with self-renewal ability and multi-directional differentiation potential. They can regenerate various tissues and human organs, so they are sometimes called "universal cells". Stem cells play an important role in the body, especially in tissue repair and regeneration. In order to isolate pure stem cell populations from complex cell mixtures for more precise research and applications.

[0003] In the prior art, stem cell sorting devices generally separate stem cells from digested tissue blocks through a filter screen. However, if the cells are not sufficiently stirred to break up cell clumps, it is easy to cause cell deposition and aggregation, thereby reducing the sorting efficiency and purity.

[0004] In view of the above problems, a stem cell sorting device for stem cell research is proposed to solve the above problems. Content of the Utility Model

[0005] In order to make up for the above deficiencies, the utility model provides a stem cell sorting device for stem cell research, aiming to improve the problems that stem cells cannot be quickly separated and cell clumps cannot be broken up in the prior art.

[0006] In order to achieve the above purpose, the utility model adopts the following technical scheme: A stem cell sorting device for stem cell research, including a bottom plate. Four support legs are fixedly connected to the left side of the top of the bottom plate. A dispersion tank is fixedly connected to the tops of the four support legs. A motor is fixedly connected to the bottom of the dispersion tank. The output end of the motor is fixedly connected with, and a plurality of stirring rods are fixedly connected to the outer periphery. A plurality of connecting rods are fixedly connected to the middle of the stirring rods. A fixing plate is fixedly connected to the far end of the connecting rod away from. Two metal rods are slidably connected to both sides inside the fixing plate. A first spring is sleeved on the outer side of the metal rod. A limiting block is fixedly connected to the near end of the first spring close to. A scraping plate is fixedly connected to the far end of the metal rod away from. A conveying pipe is fixedly connected to the right side of the dispersion tank. The far end of the conveying pipe away from the dispersion tank is fixedly connected to a collection box. A filter box is slidably connected inside the collection box. Two fixing blocks are fixedly connected to the rear side of the filter box. Two fixing components are arranged at the rear side of the collection box. The fixing components are used to quickly disassemble the filter box. A support plate is fixedly connected to the bottom of the collection box. Support columns are fixedly connected to the four peripheries of the bottom of the support plate.

[0007] As a further description of the above technical solution:

[0008] The fixed component includes a housing, the outer side of the housing is fixedly connected to the rear side of the collection box, two chutes are provided inside the housing, two insertion blocks are slidably connected inside the fixed block, a baffle is fixedly connected to one side of the insertion block, a pull rope is fixedly connected to the middle of the baffle, one end of the pull rope is fixedly connected to a second handle, a second spring is sleeved outside the pull rope, and a rotating shaft is arranged outside the pull rope.

[0009] As a further description of the above technical solution:

[0010] A feed inlet is fixedly connected to the top of the dispersion tank, and a discharge pipe is fixedly connected to the bottom of the collection box.

[0011] As a further description of the above technical solution:

[0012] One end of the first spring is fixedly connected to the side of the scraping plate away from the dispersion tank, and the other end of the first spring is fixedly connected to the side of the fixing plate away from the dispersion tank.

[0013] As a further description of the above technical solution:

[0014] The scraping plate abuts against the inner wall of the dispersion tank, and the bottom end is rotatably connected to the inner bottom wall of the dispersion tank.

[0015] As a further description of the above technical solution:

[0016] One end of the second spring is fixedly connected to the middle side of the baffle, and the other end of the second spring is fixedly connected to the inner wall of the chute.

[0017] As a further description of the above technical solution:

[0018] Both ends of the rotating shaft are rotatably connected inside the housing, and the outside of the insertion block is slidably connected inside the housing.

[0019] As a further description of the above technical solution:

[0020] A first handle is fixedly connected to the front side of the filter box.

[0021] The present utility model has the following beneficial effects:

[0022] In the present utility model, the stem cells in the dispersion tank are fully stirred, dispersed and separated by the stirring rod, so as to improve the sorting efficiency and purity. Under the action of the scraping plate, the stem cells adhered to the inner side wall of the dispersion tank are reduced, the waste is reduced, and the practicability of the device is improved.

[0023] In the present utility model, by pulling the second handle, the pull rope moves outwards accordingly, driving the baffle to slide out in the chute, the spring is compressed accordingly, and at the same time, the insertion block moves synchronously until it completely exits the inside of the fixed block, realizing the rapid separation of stem cells from the cell mixture, facilitating the collection of the separated stem cells, and improving the work efficiency. Description of the Drawings

[0024] Figure 1 Fig. is a perspective view of a stem cell sorting device for stem cell research proposed by the present utility model;

[0025] Figure 2 Fig. is a schematic structural diagram of the outer shell of a stem cell sorting device for stem cell research proposed by the present utility model;

[0026] Figure 3 Fig. is a schematic structural diagram of a scraper of a stem cell sorting device for stem cell research proposed by the present utility model;

[0027] Figure 4 Fig. is a schematic structural diagram of a filter box of a stem cell sorting device for stem cell research proposed by the present utility model;

[0028] Figure 5 Fig. is a schematic structural diagram of a baffle of a stem cell sorting device for stem cell research proposed by the present utility model.

[0029] Legend Explanation:

[0030] 1. Bottom plate; 2. Support leg; 3. Dispersion tank; 4. Motor; 5. Rotating shaft; 6. Stirring rod; 7. Connecting rod; 8. Fixed plate; 9. Metal rod; 10. First spring; 11. Limiting block; 12. Scraper; 13. Delivery pipe; 14. Collection box; 15. Filter box; 16. Fixed block; 17. Outer shell; 18. Chute; 19. Second spring; 20. Insertion block; 21. Baffle; 22. Rotating shaft; 23. Pull rope; 24. Support plate; 25. Discharge pipe; 26. Support column; 27. Feed inlet; 28. First handle; 29. Second handle. Detailed Embodiments

[0031] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0032] Refer to Figure 1 、 Figure 3 and Figure 4, an embodiment provided by the present utility model: A stem cell sorting device for stem cell research, comprising a bottom plate 1. Four support legs 2 are fixedly connected to the left side of the top of the bottom plate 1. A dispersion tank 3 is fixedly connected to the top of the four support legs 2. A motor 4 is fixedly connected to the bottom of the dispersion tank 3. The output end of the motor 4 is fixedly connected with 5. A plurality of stirring rods 6 are fixedly connected to the outer periphery of 5. A plurality of connecting rods 7 are fixedly connected to the middle of the stirring rods 6. One end of the connecting rod 7 far from 5 is fixedly connected with a fixing plate 8. Both sides of the inside of the fixing plate 8 are slidably connected with metal rods 9. A first spring 10 is sleeved on the outer side of the metal rod 9. One end of the first spring 10 close to 5 is fixedly connected with a limiting block 11. One end of the metal rod 9 far from 5 is fixedly connected with a scraping plate 12. A conveying pipe 13 is fixedly connected to the right side of the dispersion tank 3. One end of the conveying pipe 13 far from the dispersion tank 3 is fixedly connected with a collection box 14. A filter box 15 is slidably connected to the inside of the collection box 14. Two fixing blocks 16 are fixedly connected to the rear side of the filter box 15. Two fixing components are arranged at the rear side of the collection box 14. The fixing components are used for quickly disassembling the filter box 15. A support plate 24 is fixedly connected to the bottom of the collection box 14. Four support columns 26 are fixedly connected to the periphery of the bottom of the support plate 24.

[0033] Specifically, the bottom plate 1 serves as the foundation of the entire device, providing a stable support platform. The support legs 2 connect the bottom plate 1 and the dispersion tank 3, increasing the stability of the device. The dispersion tank 3 is used to accommodate the stem cell samples to be processed, and realizes the mixing and dispersion of cells through the internal stirring mechanism. The motor 4 drives the stirring mechanism to operate, providing power for cell dispersion. The stirring shaft 5 is driven by the motor 4 to rotate, driving the stirring rods 6 to perform stirring actions. The stirring rods 6 are connected to the stirring shaft and stir the cell samples by rotating to promote the uniform dispersion of cells. The connecting rods 7 connect the stirring rods 6 and the fixing plate 8, transmitting the stirring torque. The metal rods 9 slide inside the fixing plate 8, driving the scraping plates 12 to perform cleaning actions. The first spring 10 provides an elastic force, making the scraping plates closely adhere to the inner wall of the dispersion tank 3, effectively removing the attached cells. The limiting blocks 11 limit the moving range of the metal rods 9, ensuring the normal operation of the scraping plates 12. The scraping plates 12 clean the inner wall of the dispersion tank 3 as the metal rods 9 slide, preventing cell adhesion. The conveying pipe 13 connects the dispersion tank 3 and the collection box 14, and is used to convey the processed cell samples to the collection box 14. The filter box 15 is located inside the collection box 14 and is used for filtering and separating different components in the cell samples. The fixing blocks 16 cooperate with the fixing components to quickly install or disassemble the filter box 15. The support plate 24 provides additional support for the collection box.

[0034] Refer to Figure 2 、 Figure 4 and Figure 5, The fixing component includes a housing 17, the outside of the housing 17 is fixedly connected to the rear side of the collection box 14, two sliding grooves 18 are opened inside the housing 17, two plug-in blocks 20 are slidably connected inside the fixing block 16, a baffle 21 is fixedly connected to one side of the plug-in block 20, a pull rope 23 is fixedly connected to the middle of the baffle 21, one end of the pull rope 23 is fixedly connected to a second handle 29, a second spring 19 is sleeved outside the pull rope 23, and a rotating shaft 22 is arranged outside the pull rope 23.

[0035] Specifically, the housing 17 is fixed to the rear side of the collection box 14, providing a protective and supporting structure for other parts of the fixing component. The sliding grooves 18 are opened inside the housing 17, providing a sliding path for the plug-in blocks 20, enabling the plug-in blocks 20 to smoothly move inside the fixing block 16. The second spring 19 is sleeved outside the pull rope 23, providing an elastic force, enabling the plug-in blocks 20 to automatically rebound to their original positions after being pulled out, thereby fixing the filter box 15. The plug-in blocks 20 slide inside the fixing block 16, and through cooperation with the sliding grooves 18, the fixing or release of the filter box 15 is achieved. The baffle 21 is fixedly connected to one side of the plug-in block 16, possibly used to prevent the plug-in block 16 from sliding out excessively and keeping it in an appropriate position. The rotating shaft 22 is used to support and guide the movement of the pull rope 23, possibly to change the direction of the pull rope 23 or reduce friction. One end of the pull rope 23 is connected to the baffle 21, and the other end is connected to the second handle 29. By pulling the second handle 29, the movement of the plug-in blocks 20 in the sliding grooves 18 can be controlled, thereby achieving the fixing or release of the filter box 15.

[0036] Refer to Figure 1 , Figure 3 and Figure 5 , a feed inlet 27 is fixedly connected to the top of the dispersion tank 3, a discharge pipe 25 is fixedly connected to the bottom of the collection box 14, one end of the first spring 10 is fixedly connected to the side of the scraping plate 12 away from the dispersion tank 3, the other end of the first spring 10 is fixedly connected to the side of the fixing plate 8 away from 5, the scraping plate 12 abuts against the inner wall of the dispersion tank 3, the 5 bottom end is rotatably connected to the inner bottom wall of the dispersion tank 3, one end of the second spring 19 is fixedly connected to the middle side of the baffle 21, the other end of the second spring 19 is fixedly connected to the inner wall of the sliding groove 18, both ends of the rotating shaft 22 are rotatably connected inside the housing 17, the outside of the plug-in block 20 is slidably connected inside the housing 17, and a first handle 28 is fixedly connected to the front side of the filter box 15.

[0037] Specifically, the discharge pipe 25 is fixed to the bottom of the collection box 14 and is used to discharge the cell samples that have been filtered and sorted, facilitating collection and further processing. The feed inlet 27 is fixed to the top of the dispersion tank 3 and is used to inject the stem cell samples to be processed into the dispersion tank 3 for stirring and dispersion. One end of the first spring 10 is fixed to the side of the scraper away from the dispersion tank 3, and the other end is fixed to the side of the fixed plate 8 away from the stirring shaft 5. The function of the first spring 10 is to keep the scraper 12 in close contact with the inner wall of the dispersion tank 3, ensuring that the cells attached to the inner wall can be effectively removed during the stirring process, preventing cell adhesion and accumulation. The bottom end of the stirring shaft 5 is rotatably connected to the inner bottom wall of the dispersion tank 3. Driven by the motor 4, the stirring shaft 5 drives the stirring rod 6 to rotate, realizing uniform stirring and dispersion of the cell samples. One end of the second spring 19 is fixed to the middle of the baffle 21, and the other end is fixed to the inner wall of the chute 18. The function of the second spring 19 is to push the insertion block 20 back to its original position when the pull rope 23 is relaxed, thereby fixing the filter box 15. The first handle 28 is fixed to the front side of the filter box 15, and the operator can pull the first handle 28 to take out or put in the filter box 15, facilitating the replacement and maintenance of the filter box 15.

[0038] Working principle: Pour the cell mixture into the interior of the dispersion tank 3 from the feed inlet 27, start the motor 4 to drive the rotation of the rotating shaft 5, and then drive the rotation of the stirring rod 6 and the scraper 12 to fully stir and break up the cell clusters in the cell mixture in the dispersion tank 3. Under the action of the scraper 12, the waste of stem cells adhering to the inner side wall of the dispersion tank 3 is reduced. If the scraper 12 is worn, the first spring 10 will play its role to ensure that the scraper 12 is closely attached to the inner wall of the dispersion tank 32. After the breaking-up is completed, the cell mixture will be transported to the interior of the collection box 14 through the delivery pipe 13 and flow into the interior of the filter box 15. Under the action of the filter box 15, the cell mixture flows through the filter holes at the bottom of the filter box 15 into the discharge pipe 25 and is discharged, while the stem cells are left inside the filter box 15. When it is necessary to take out the separated stem cells, first pull the second handle 29, which then drives the pull rope 23 to move outwards, then drives the baffle 21 to move inside the chute 18, compressing the second spring 19 at the same time. The insertion block 20 also moves inside the chute 18 until the insertion block 20 completely leaves the inside of the fixing block 16. At this time, the filter box 15 can be taken out, and the disassembly is completed. When installation is required, put the filter box 15 into the interior of the collection box 14 and align the fixing block 16 with the card slot inside the housing 17. At this time, release the second handle 29. Under the action of the second spring 19, the baffle 21 is pushed to slide inside the chute 18, and then the insertion block 20 is re-inserted into the inside of the fixing block 16, and the installation is completed at this time.

[0039] Finally, it should be noted that the above are only the preferred embodiments of the present utility model and are not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A stem cell sorting device for stem cell research, comprising a base plate (1), characterized in that: Four supporting legs (2) are fixedly connected to the left side of the top of the bottom plate (1), and a dispersion tank (3) is fixedly connected to the top of the four supporting legs (2). A motor (4) is fixedly connected to the bottom of the dispersion tank (3), and a motor (5) is fixedly connected to the output end of the motor (4). A plurality of stirring rods (6) are fixedly connected to the outer periphery of the (5), and a plurality of connecting rods (7) are fixedly connected to the middle of the stirring rods (6). The connecting rods (7) are fixedly connected to a fixed plate (8) at one end away from the (5), and metal rods (9) are slidably connected to both sides of the inside of the fixed plate (8). A spring (10) is sleeved on the outer side of the metal rod (9), and the spring (10) is fixedly connected to a limit block at one end close to the (5). (11), the end of the metal rod (9) away from the (5) is fixedly connected to a scraper (12), the right side of the dispersion tank (3) is fixedly connected to a delivery pipe (13), the end of the delivery pipe (13) away from the dispersion tank (3) is fixedly connected to a collection box (14), the interior of the collection box (14) is slidably connected to a filter box (15), the rear side of the filter box (15) is fixedly connected to two fixed blocks (16), the rear side of the collection box (14) is provided with two fixed components, the fixed components are used to quickly disassemble the filter box (15), the bottom of the collection box (14) is fixedly connected to a support plate (24), and the bottom of the support plate (24) is fixedly connected to support columns (26) on all sides.

2. A stem cell sorting device for stem cell research according to claim 1, characterized in that: The fixing assembly comprises a shell (17), the outer side of the shell (17) is fixedly connected to the rear side of the collection box (14), the inner side of the shell (17) is provided with two slide grooves (18), the inner side of the fixing block (16) is slidably connected with two plug-in blocks (20), one side of the plug-in block (20) is fixedly connected with a baffle (21), the middle part of the baffle (21) is fixedly connected with a pull rope (23), one end of the pull rope (23) is fixedly connected with a second handle (29), the outer side of the pull rope (23) is provided with a second spring (19), and the outer side of the pull rope (23) is provided with a rotating shaft (22).

3. A stem cell sorting device for stem cell research according to claim 1, characterized in that: The top of the dispersion tank (3) is fixedly connected to a feed port (27), and the bottom of the collection box (14) is fixedly connected to a discharge pipe (25).

4. A stem cell sorting device for stem cell research according to claim 1, characterized in that: One end of the spring (10) is fixedly connected to a side of the scraper (12) away from the dispersion tank (3), and the other end of the spring (10) is fixedly connected to a side of the fixed plate (8) away from the (5).

5. A stem cell sorting device for stem cell research according to claim 1, characterized in that: The scraper (12) is in contact with the inner wall of the dispersion tank (3), and the bottom end of the (5) is rotatably connected to the inner bottom wall of the dispersion tank (3).

6. A stem cell sorting device for stem cell research according to claim 2, characterized in that: One end of the second spring (19) is fixedly connected to the middle side of the baffle (21), and the other end of the second spring (19) is fixedly connected to the inner wall of the slide groove (18).

7. A stem cell sorting device for stem cell research according to claim 2, characterized in that: Both ends of the rotating shaft (22) are rotatably connected to the inside of the housing (17), and the outside of the plug-in block (20) is slidably connected to the inside of the housing (17).

8. A stem cell sorting device for stem cell research according to claim 1, characterized in that: A handle 1 (28) is fixedly connected to the front side of the filter box (15).

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