Suspension culture auxiliary equipment for cell culture bag
By designing a variety of shaking methods and adjustable tilt cell culture bag auxiliary equipment, the problems of uneven cell distribution and unwell growth requirements in traditional cell culture are solved, and more efficient cell expansion and growth quality is achieved.
Patent Information
- Application Number
- CN202421926405.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-09
- Publication Date
- 2025-06-20
- Estimated Expiration
- 2034-08-09
AI Technical Summary
The traditional immune cell culture method has the problem of uneven cell distribution and inability to adapt to cell growth needs, resulting in limited cell expansion efficiency and growth quality.
A suspension culture auxiliary device for cell culture bags is designed, using multiple shaking methods and adjustable tilts, creating a more uniform and suitable cell growth environment through the combination of horizontal shaking, left and right shaking and lifting.
The uniform distribution and growth of cells in the middle area of the culture bag is achieved, the cell expansion efficiency and culture effect are improved, and the need for suspension culture of immune cells is met.
Smart Images
Figure CN223002946U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of medical devices, and specifically to an auxiliary device for suspension culture of cell culture bags. Background Art
[0002] In the field of immune cell culture, there are many problems with traditional culture methods. Generally, without horizontal shaking, due to gravity, immune cells will all settle at the bottom of the culture bag. This results in the cells in the bottom layer of the culture bag having very little contact with the culture medium and being unable to fully obtain nutrients, while the cells in the top layer have very little contact with carbon dioxide, which is not conducive to cell metabolism and growth. This uneven distribution seriously affects the suspension culture effect of cells, greatly limiting the cell amplification efficiency and growth quality. Moreover, if the shaking rate is too high, it will also have an adverse effect on cell growth because too high a shaking rate does not meet the state requirements of cell resting growth. In special cases, when cells settle on one side of the culture bag, conventional horizontal shaking cannot solve this problem. At this time, a shaking method of back and forth left and right and up and down is required to promote the distribution and growth of cells in the middle area of the culture bag to meet the ideal conditions for cell culture.
[0003] In summary, traditional immune cell culture methods have problems such as uneven cell distribution and inability to adapt to cell growth requirements. There is an urgent need for a new type of culture device that can effectively solve these problems to improve the cell amplification efficiency and culture effect and meet the needs of immune cell suspension culture. Summary of the Utility Model
[0004] The purpose of the utility model is to provide an auxiliary device for suspension culture of cell culture bags to provide a more comprehensive and uniform suspension culture environment for cells to solve the above problems.
[0005] To achieve the above object, the present utility model provides the following technical solution: A suspension culture auxiliary device for a cell culture bag, comprising an incubator; the four corners of the bottom of the incubator are respectively fixedly connected to the upper end of a lifting motor through a lifting spring bracket, the lifting motor is fixedly installed on the upper end of a left - right shaking panel, both sides of the bottom end of the left - right shaking panel are respectively provided with side plates, and one end of the side plate is provided with an opening. The left - right shaking panel is slidably installed in a chute provided at the upper end of the side wall of a left - right shaking box through the side plates at the bottom. A motor bracket is fixedly installed inside the bottom of the left - right shaking box, a first motor is fixedly installed on the motor bracket, and a first turntable is fixed on the rotating shaft of the motor. The outer - side connecting shaft on the side wall of the turntable is rotatably connected to the bottom of a T - shaped connecting rod, and both ends of the T - shaped connecting rod are rotatably connected to the opening at one end of the side plate; the bottom end of the left - right shaking box is placed on the upper end of a horizontal shaking box. A second motor is fixedly installed at the center of the inner bottom surface of the horizontal shaking box, a second turntable is fixedly installed on the rotating shaft of the second motor, a fixed shaft is fixed at the short - side of the inner bottom surface of the horizontal shaking box, a chute rod is rotatably installed at the top end of the fixed shaft, a slider is slidably installed in the chute of the chute rod, the upper end of the slider is fixed at the center of the bottom end of the left - right shaking box, and an opening is provided at the center of the slider. The opening is rotatably connected to the outer - side connecting shaft on the side wall of the turntable.
[0006] The incubator is a rectangular frame, and the frame walls are all provided with openings.
[0007] Clasps are provided at the four corners inside the incubator, and the clasps are matched with the openings at the four corners of the culture bag.
[0008] Straps are provided on the four sides inside the incubator. The straps converge at the upper end of the center of the incubator and are connected through a circular clasp. The straps are made of elastic material.
[0009] A control panel is installed outside the horizontal shaking box, and the control panel is electrically connected to the lifting spring bracket, the lifting motor, the first motor, and the second motor through wires.
[0010] A power cord connector is provided outside the control panel, and the power cord connector is connected to a power source through a power cord.
[0011] The control panel is provided with a timing switch function. By turning on the timing switch on the control panel and setting the time, the device can be turned off at a fixed time.
[0012] First, place the culture bag into the incubator of the rectangular frame. Use the buckles at the four corners inside the incubator to fasten the openings at the four corners of the culture bag, and then tie it to the culture bag with elastic straps on the four sides inside the incubator. The straps are connected and fixed through a circular buckle at the upper end of the center of the incubator. Next, connect the power supply connector to the power supply through the power cord, and operate the device by horizontally shaking the control panel on the outside of the box. Press the horizontal shaking key to turn on the timing switch and set the time. After the second motor inside the horizontal shaking box starts, its rotating shaft drives the turntable to rotate. Since the opening of the slider is rotationally connected to the outer connecting shaft on the side wall of the turntable, the rotation of the turntable will drive the slider to move. At the same time, the chute rod rotatably installed at the top of the fixed shaft restricts the movement track of the slider, so that it can only slide within the chute of the chute rod. When the slider moves under the combined action of the turntable and the chute rod, because the upper end of the slider is fixed at the center of the bottom end of the left and right shaking box, it will drive the left and right shaking box to perform horizontal shaking motion. The shaking rate is 10 rpm / min, and it is horizontal shaking for 99% of the time. When it is found that the suspended cells are deposited on one side, operate the device through the control panel. Press the left and right shaking key and the tilt key, turn on the timing switch and set the time. At this time, after the first motor inside the bottom of the left and right shaking box starts, its rotating shaft drives the turntable to rotate. Since the outer connecting shaft on the side wall of the turntable is rotationally connected to the bottom of the T-shaped connecting rod, the rotation of the turntable will drive the T-shaped connecting rod to move. The two ends of the T-shaped connecting rod are also rotationally connected to the openings at one end of the side plates on both sides of the bottom end of the left and right shaking panel. When the T-shaped connecting rod moves, it will push the side plates, and then drive the left and right shaking panel to move. The left and right shaking panel cooperates with the chute provided at the upper end of the side wall of the left and right shaking box through the side plates at the bottom, so that the left and right shaking panel can stably move left and right under the restriction of the chute. At the same time, the lifting spring brackets cooperate with the lifting at the same time. By raising the left lifting spring bracket or the right lifting spring bracket respectively, the incubator is tilted 30 degrees left and right, so that the cells grow and distribute in the middle area of the culture bag. After the suspension immune cell culture is completed, take out this device from the incubator and continue with the subsequent experiments.
[0013] Compared with the prior art, the beneficial effects of the present utility model are:
[0014] Through horizontal shaking, left and right shaking, and adjustable tilting, this device can provide a more comprehensive and uniform suspension culture environment for cells. Compared with the prior art that may only have a single shaking method, it is more conducive to the uniform growth and distribution of cells.
[0015] It is equipped with a control panel, which can realize the selection of different shaking modes, timing switch, and control of the shaking rate.
[0016] The buckles and straps inside the incubator can firmly fix the culture bag, prevent it from shifting or being damaged during the shaking process, and ensure the stability and reliability of the culture.
[0017] Combining multiple shaking methods and precise control can better simulate the growth environment of cells in the body, reduce cell deposition and aggregation, and thus improve the quality and success rate of cell culture.
[0018] The integrated design and simple operation process enable various settings to be completed through the control panel, reducing the complexity and difficulty of operation and improving work efficiency. Brief Description of the Drawings
[0019] Figure 1 It is a three-dimensional view of the main structure of an auxiliary device for suspension culture of a cell culture bag of the present utility model;
[0020] Figure 2 It is a three-dimensional view of the main structure of the incubator of an auxiliary device for suspension culture of a cell culture bag of the present utility model;
[0021] Figure 3 It is a sectional view of the internal structure of the left and right shaking box of an auxiliary device for suspension culture of a cell culture bag of the present utility model;
[0022] Figure 4 It is a sectional view of the internal structure of an auxiliary device for suspension culture of a cell culture bag of the present utility model;
[0023] Figure 5 It is a sectional view of the internal structure of the horizontal shaking box of an auxiliary device for suspension culture of a cell culture bag of the present utility model;
[0024] In the figure: 11, incubator; 12, buckle; 13, strap; 14, circular buckle; 21, lifting spring bracket; 22, lifting motor; 31, left and right shaking panel; 311, side plate; 32, left and right shaking box; 33, motor bracket; 34, first motor; 341, first turntable; 35, T-shaped connecting rod; 41, horizontal shaking box; 42, second motor; 43, second turntable; 44, chute rod; 45, slider; 46, fixed shaft; 5, control panel. Detailed Embodiments
[0025] Next, the technical solutions in the embodiments of the present utility model will be completely described in conjunction with the accompanying drawings in the embodiments of the present utility model.
[0026] Such as Figures 1-5As shown in the figure, a suspension culture auxiliary device for a cell culture bag includes an incubator 11. The four corners of the bottom of the incubator 11 are respectively fixedly connected to the upper end of a lifting spring bracket 21, and the lifting spring bracket 21 is fixedly installed on the upper end of a left-right shaking panel 31. Both sides of the bottom end of the left-right shaking panel 31 are respectively provided with side plates 311, and one end of the side plate 311 is provided with an opening. The left-right shaking panel 31 is slidably installed in a chute provided at the upper end of the side wall of a left-right shaking box 32 through the side plates 311 at the bottom. Inside the bottom of the left-right shaking box 32, a motor bracket 33 is fixedly installed. A first motor 34 is fixedly installed on the motor bracket 33, and a first turntable 341 is fixed on the rotating shaft of the motor. The bottom of a T-shaped connecting rod 35 is rotationally connected to the outer side connecting shaft on the side wall of the first turntable 341. Both ends of the T-shaped connecting rod 35 are rotationally connected to the openings at one end of the side plates 311 in a matching manner. The bottom end of the left-right shaking box 32 is placed on the upper end of a horizontal shaking box 41. Inside the center of the bottom surface of the horizontal shaking box 41, a second motor 42 is fixedly installed. A second turntable 43 is fixed on the rotating shaft of the second motor 42. A fixed shaft 46 is fixed at the short side of the bottom surface inside the horizontal shaking box 41. The top end of the fixed shaft 46 is rotatably installed with a chute rod 44. A slider 45 is slidably installed in the chute of the chute rod 44. The upper end of the slider 45 is fixed at the center of the bottom end of the left-right shaking box 32, and an opening is provided at the center of the slider 32. The opening is rotationally connected to the outer side connecting shaft on the side wall of the second turntable 43.
[0027] The incubator 11 is a rectangular frame, and openings are provided on all the frame walls.
[0028] At the four corners inside the incubator 11, buckles 12 are provided, and the buckles 12 are matched with the openings at the four corners of the culture bag.
[0029] On the four sides inside the incubator 11, straps 13 are provided. The straps 13 converge at the upper end of the center of the incubator 11 and are connected through a circular buckle 14. The straps 13 are made of elastic material.
[0030] A control panel 5 is installed outside the horizontal shaking box 41, and the control panel 5 is electrically connected to the lifting spring bracket 21, the lifting motor 22, the first motor 34, and the second motor 42.
[0031] A power cord connector is provided outside the control panel 5, and the power cord connector is connected to a power source through a power cord.
[0032] The control panel 5 is provided with a timing switch function. By turning on the timing switch on the control panel 5 and setting the time, the device can be turned off at a set time.
[0033] First, place the culture bag into the incubator 11 of the rectangular frame. Use the fasteners 12 at the four corners inside the incubator 11 to fasten the openings at the four corners of the culture bag, and then tie it to the culture bag with the elastic straps 13 on the four sides inside the incubator 11. The straps 13 are connected and fixed through the circular fastener 14 at the upper end of the center of the incubator 11. Next, connect the power supply connector to the power supply through the power cord, and operate the device by shaking the control panel 5 on the outside of the horizontal shaking box 41. Press the horizontal shaking key to turn on the timing switch and set the time. After the second motor 42 in the horizontal shaking box 41 starts, its rotating shaft drives the second turntable 43 to rotate. Since the opening of the slider 45 is rotatably connected to the outer connecting shaft on the side wall of the second turntable 43, the rotation of the second turntable 43 will drive the slider 45 to move. At the same time, the chute rod 44 rotatably installed at the top of the fixed shaft 46 restricts the movement track of the slider 45, so that it can only slide in the chute of the chute rod 44. When the slider 45 moves under the combined action of the second turntable 43 and the chute rod 44, because the upper end of the slider 45 is fixed at the center of the bottom end of the left and right shaking box 32, it will drive the left and right shaking box 32 to perform horizontal shaking movement. The shaking rate is 10 rpm / min, and it is horizontal shaking for 99% of the time. When it is found that the suspended cells are deposited on one side, operate the device through the control panel 5. Press the left and right shaking key and the tilt key, turn on the timing switch and set the time. At this time, after the first motor 34 at the inner bottom of the left and right shaking box 32 starts, its rotating shaft drives the first turntable 341 to rotate. Since the outer connecting shaft on the side wall of the first turntable 341 is rotatably connected to the bottom of the T-shaped connecting rod 35, the rotation of the first turntable 341 will drive the T-shaped connecting rod 35 to move. The two ends of the T-shaped connecting rod 35 are also rotatably connected to the openings at one end of the side plates 311 on both sides of the bottom end of the left and right shaking panel 31. When the T-shaped connecting rod 35 moves, it will push the side plates 311, and then drive the left and right shaking panel 31 to move. The left and right shaking panel 31 cooperates with the chute provided at the upper end of the side wall of the left and right shaking box 32 through the side plates 311 at the bottom, so that the left and right shaking panel 31 can move stably left and right under the restriction of the chute. At the same time, the lifting spring brackets 21 cooperate with each other to lift. By raising the left lifting spring bracket 21 or the right lifting spring bracket 21 respectively, the incubator 11 is tilted 30 degrees left and right, so that the cells grow distributed in the middle area of the culture bag. After the suspension immune cell culture is completed, take out this device from the incubator and continue with the subsequent experiments.
Claims
1. A suspension culture auxiliary device for a cell culture bag, characterized in that: The invention comprises an incubator (11); the four corners of the bottom of the incubator (11) are respectively fixedly connected to the upper end of a lifting motor (22) through a lifting spring bracket (21); the lifting motor (22) is fixedly installed on the upper end of a left-right shaking panel (31); the bottom ends of the left-right shaking panel (31) are respectively provided with side panels (311), and one end of the side panels (311) is provided with an opening; the left-right shaking panel (31) is slidably installed in a slide groove provided at the upper end of the side wall of the left-right shaking box (32) through the side panels (311) at the bottom; a motor bracket (33) is fixedly installed on the inner side of the bottom of the left-right shaking box (32); a first motor (34) is fixedly installed on the motor bracket (33); a first turntable (341) is fixedly installed on the rotating shaft of the motor; and the first turntable (341) is rotatably connected to the first turntable (341) through a connecting shaft on the outer side of the side wall of the first turntable (341). The bottom of the T-shaped connecting rod (35), the two ends of the T-shaped connecting rod (35) are rotatably connected with the opening at one end of the side plate (311); the bottom end of the left and right shaking box (32) is placed on the upper end of the horizontal shaking box (41), and a second motor (42) is fixedly installed at the inner center of the bottom surface of the horizontal shaking box (41), and a second turntable (43) is fixedly installed on the rotating shaft of the second motor (42), and a fixed shaft (46) is fixed at the inner short side of the bottom surface of the horizontal shaking box (41), and a slide groove rod (44) is rotatably installed at the top of the fixed shaft (46), and a slider (45) is slidably installed in the slide groove of the slide groove rod (44), and the upper end of the slider (45) is fixed at the bottom center of the left and right shaking box (32), and an opening is provided in the center of the slider (45), and the opening is rotatably connected with the outer connecting shaft of the side wall of the second turntable (43).
2. The suspension culture auxiliary device for a cell culture bag according to claim 1, characterized in that: The incubator (11) is a rectangular frame, and the frame walls are all provided with openings.
3. The suspension culture auxiliary device for a cell culture bag according to claim 1, characterized in that: Buckles (12) are arranged at the four inner corners of the culture box (11), and the buckles (12) match the openings at the four corners of the culture bag.
4. The suspension culture auxiliary device for a cell culture bag according to claim 1, characterized in that: The four inner sides of the incubator (11) are provided with binding straps (13), the binding straps (13) intersect at the upper end of the center of the incubator (11) and are connected by a circular buckle (14), and the binding straps (13) are made of elastic material.
5. The suspension culture auxiliary device for a cell culture bag according to claim 1, characterized in that: A control panel (5) is installed on the outside of the horizontal shaking box (41), and the control panel (5) is connected to the lifting spring bracket (21), the lifting motor (22), the first motor (34), and the second motor (42) through electric wires.
6. The suspension culture auxiliary device for a cell culture bag according to claim 5, characterized in that: The control panel (5) is connected to a power cord connector on the outside thereof, and the power cord connector is connected to a power source via a power cord.
7. The suspension culture auxiliary device for a cell culture bag according to claim 5, characterized in that: The control panel (5) is provided with a timer switch function. By turning on the timer switch and setting the time through the control panel (5), the device can be shut down at a specified time.