Radial flow convection chromatography column

Abstract

The utility model discloses a radial flow convection chromatographic column, and particularly relates to the technical field of convection chromatographic columns, the radial flow convection chromatographic column comprises a convection chromatographic column body, a first filter tank is arranged at the bottom end of the convection chromatographic column body, and a second filter tank is arranged in the first filter tank. According to the convection chromatographic column, the first filter tank is arranged on the outer wall of the convection chromatographic column body, so that the bottom cover is conveniently separated from the convection chromatographic column body, when the convection chromatographic column is connected with a protein purification instrument through a pipeline, filler needs to be added into the convection chromatographic column, and when subsequent liquid passes through the inside of the convection chromatographic column, the filler is not required to be added into the convection chromatographic column. When the liquid passes through the convection chromatographic column, the liquid is shunted through the gear block between the first filter tank and the second filter tank, so that the liquid is easy to be in full contact with the filler, and the purity of the liquid subjected to subsequent processing is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of convection chromatography columns, in particular to a radial flow convection chromatography column. Background Art

[0002] The protein purifier is designed for the fully automatic two-step affinity purification of proteins with His peptide tags and GST peptide tags. The Profinia protein purification system is designed for the fully automatic two-step affinity purification of proteins with His (His-Tagged) peptide tags and GST (GSTtagged) peptide tags. The convenient integrated system is superior to the traditional gravity flow affinity chromatography technology and is used for the desalting of purified and affinity-labeled proteins. The chromatography method program is pre-programmed in the system. Together with the Profinia purification and buffer kit, independent and reproducible results are produced. Without tube operation, the Profinia protein purification system can obtain purified samples in less than 30 minutes for downstream research.

[0003] At present, when using a protein purifier on the market, it is necessary to install the convection chromatography column on the protein purifier and then connect it to the convection chromatography column through subsequent pipelines. In actual use, when the convection chromatography column is connected to the protein purifier through the pipeline, it is also necessary to add fillers inside the convection chromatography column. When the subsequent liquid passes through the inside of the convection chromatography column, the liquid can contact and react with the fillers. However, when most liquids pass through the inside of the convection chromatography column, the liquid is directly introduced into the inside of the convection chromatography column, making it easy for the liquid to have incomplete chemical reactions with the fillers, resulting in a decrease in the purity of the subsequent processed liquid. Summary of the Utility Model

[0004] The purpose of the present utility model is to provide a radial flow convection chromatography column to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present utility model provides the following technical solutions:

[0006] A radial flow convection chromatography column, including a convection chromatography column body. A first filtration groove is opened at the bottom end of the convection chromatography column body. A second filtration groove is opened inside the first filtration groove. A gear block is fixedly connected at the connection between the first filtration groove and the second filtration groove. A filling piece is arranged inside the second filtration groove. A bottom cover is arranged at the bottom end of the convection chromatography column body. A connection groove is opened at the bottom end of the bottom cover. An installation groove corresponding to the connection groove is opened at the bottom end of the convection chromatography column body. A threaded bolt is arranged inside the connection groove and one end of the threaded bolt is connected to the installation groove through threads.

[0007] Furthermore, connection holes are provided at the top of the convection chromatography column body and the bottom end of the bottom cover, and the connection holes communicate with the second filter tank. The addition of the connection holes enables the connection pipe to communicate with the second filter tank.

[0008] Furthermore, one end of the connection pipe away from the connection hole is fixedly connected with a mounting disc. A pipe groove is provided on the outer wall of the mounting disc on the side away from the connection pipe. A groove is provided inside the mounting disc on one side of the pipe groove. The addition of the mounting disc enables the connection pipe to move inside the connection hole.

[0009] Furthermore, a fixing rod is fixedly connected inside the groove. A moving plate is slidably connected to the outer wall of the fixing rod. A positioning rod is fixedly connected to the outer wall of the moving plate. One end of the positioning rod penetrates into the pipe groove and the positioning rod is slidably connected with the mounting disc. A pipe body is slidably connected inside the pipe groove. A positioning groove is provided on the outer wall of the pipe body and one end of the positioning rod is slidably connected with the positioning groove. The addition of the positioning rod facilitates the fixing of the position of the pipe body.

[0010] Furthermore, a spring is fixedly connected to the outer wall of the moving plate. A pressure receiving plate is fixedly connected to the outer wall of the moving plate on one side of the positioning rod. The addition of the spring enables the moving plate to return to the initial position.

[0011] Furthermore, a pull rod is provided inside the groove. An extrusion rod is fixedly connected to the outer wall of the pull rod. One end of the extrusion rod is hinged with a roller. The addition of the extrusion rod enables the pull rod to drive the roller to move.

[0012] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0013] 1. The radial flow convection chromatography column of the present utility model can enable the liquid to fully undergo a chemical reaction with the internal filling sheets. By providing a first filter tank on the outer wall of the convection chromatography column body, a second filter tank inside the first filter tank, a gear block between the first filter tank and the second filter tank, a connection groove on the outer wall of the bottom cover, a threaded bolt inside the connection groove, and a mounting groove at the bottom end of the convection chromatography column body, the bottom cover can be conveniently separated from the convection chromatography column body. In the present utility model, when the convection chromatography column is connected to a protein purifier through a pipeline, fillers need to be added inside the convection chromatography column. When the subsequent liquid passes through the inside of the convection chromatography column, the liquid can contact and react with the fillers. When the liquid passes through the inside of the convection chromatography column, it will be shunted by the gear block between the first filter tank and the second filter tank, enabling the liquid to easily come into full contact with the fillers, resulting in an improvement in the purity of the subsequent processed liquid.

[0014] 2. The radial flow convection chromatography column of the present utility model enables convenient installation and disassembly between the pipe body and the convection chromatography column body. By providing an extrusion rod on the outer wall of the pull rod, a roller at one end of the extrusion rod, a moving plate on the outer wall of the fixed rod, a pressure receiving plate on the outer wall of the moving plate, a positioning rod on the outer wall of the moving plate, and a spring between the outer wall of the moving plate and the groove, the moving plate can conveniently return to its initial position. In the present utility model, the end of the pipe body with a positioning groove is inserted into the pipe groove. At this time, the pull rod is pulled, making the subsequent connection and disassembly between the pipe body and the convection chromatography column body convenient and fast. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The drawings are used to provide a further understanding of the present utility model and constitute a part of the specification. Together with the embodiments of the present utility model, they are used to explain the present utility model and do not constitute a limitation to the present utility model. In the drawings:

[0016] Figure 1 is a schematic diagram of the overall structure of the present utility model;

[0017] Figure 2 is a schematic diagram of the overall structure of the mounting plate of the present utility model;

[0018] Figure 3 is a schematic diagram of the overall structure of the gear block of the present utility model;

[0019] Figure 4 is a top view cross-sectional view of the groove of the present utility model.

[0020] In the figure: 1. Convection chromatography column body; 2. First filter tank; 3. Second filter tank; 4. Gear block; 5. Filling sheet; 6. Bottom cover; 7. Connection groove; 8. Installation groove; 9. Threaded bolt; 10. Connection hole; 11. Connection pipe; 12. Mounting plate; 13. Pipe groove; 14. Groove; 15. Fixed rod; 16. Moving plate; 17. Positioning rod; 18. Pipe body; 19. Positioning groove; 20. Spring; 21. Pressure receiving plate; 22. Pull rod; 23. Extrusion rod; 24. Roller. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present utility model with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0022] Please refer to Figure 1 , Figure 2 and Figure 3 , the present utility model provides a radial flow convection chromatography column, and the technical solutions are as follows:

[0023] Radial flow countercurrent chromatography column, including a countercurrent chromatography column body 1. A first filter tank 2 is provided at the bottom end of the countercurrent chromatography column body 1. A second filter tank 3 is provided inside the first filter tank 2. A gear block 4 is fixedly connected at the connection between the first filter tank 2 and the second filter tank 3. A filling piece 5 is provided inside the second filter tank 3. A bottom cover 6 is provided at the bottom end of the countercurrent chromatography column body 1. A connection groove 7 is provided at the bottom end of the bottom cover 6. An installation groove 8 is provided at the bottom end of the countercurrent chromatography column body 1 corresponding to the connection groove 7. A threaded bolt 9 is provided inside the connection groove 7 and one end of the threaded bolt 9 is connected to the installation groove 8 by threads.

[0024] In a preferred embodiment, connection holes 10 are provided at the top of the countercurrent chromatography column body 1 and the bottom end of the bottom cover 6, and the connection holes 10 communicate with the second filter tank 3. A connection pipe 11 is slidably connected inside the connection holes 10. When the connection pipe 11 moves, the connection pipe 11 can move inside the connection holes 10, so that the subsequent connection pipe 11 can communicate with the second filter tank 3.

[0025] In a preferred embodiment, a mounting disc 12 is fixedly connected to one end of the connection pipe 11 away from the connection hole 10. A pipe groove 13 is provided on the outer wall of the mounting disc 12 on the side away from the connection pipe 11. A groove 14 is provided inside the mounting disc 12 on one side of the pipe groove 13. When the mounting disc 12 moves, the mounting disc 12 can drive the connection pipe 11 to move, and the subsequent connection pipe 11 can move inside the connection hole 10.

[0026] In a preferred embodiment, a fixing rod 15 is fixedly connected inside the groove 14. A moving plate 16 is slidably connected to the outer wall of the fixing rod 15. A positioning rod 17 is fixedly connected to the outer wall of the moving plate 16. One end of the positioning rod 17 penetrates inside the pipe groove 13 and the positioning rod 17 is slidably connected to the mounting disc 12. A pipe body 18 is slidably connected inside the pipe groove 13. A positioning groove 19 is provided on the outer wall of the pipe body 18 and one end of the positioning rod 17 is slidably connected to the positioning groove 19. When the moving plate 16 moves, the moving plate 16 can move outside the fixing rod 15, so that the moving plate 16 can drive the positioning rod 17 to move.

[0027] Working principle of the utility model: When the convection chromatography column body 1 is installed on a protein purifier, the pipeline body 18 is respectively connected to the pipeline grooves 13 outside the mounting disks 12 installed on both sides of the chromatography column body. At this time, the liquid enters the first filtering tank 2 from the mounting disk 12 at the large end of the chromatography column body through the connecting pipe 11. When the liquid enters the second filtering tank 3 from the first filtering tank 2, the liquid will pass through the gear blocks 4 between the first filtering tank 2 and the second filtering tank 3. The multiple gear blocks 4 between the first filtering tank 2 and the second filtering tank 3 will play a role in diverting the liquid, enabling the subsequent liquid to enter the second filtering tank 3 evenly, so that the liquid can fully contact with the filling sheets 5 inside the second filtering tank 3 to undergo a chemical reaction. The reacted liquid can be removed from the connecting hole 10 at the small head part outside the convection chromatography column body 1. After the liquid enters the second filtering tank 3, it can fully react chemically with the internal filling sheets 5, improving the purity.

[0028] Please refer to Figure 1 , Figure 2 and Figure 4 , the utility model provides a technical solution: A spring 20 is fixedly connected to the outer wall of the moving plate 16, and a pressure receiving plate 21 is fixedly connected to the outer wall of the moving plate 16 on one side of the positioning rod 17. When the moving plate 16 moves, the moving plate 16 can squeeze the spring 20, and at the same time, the moving plate 16 will move inside the groove 14.

[0029] In a preferred embodiment, a pull rod 22 is provided inside the groove 14. An extrusion rod 23 is fixedly connected to the outer wall of the pull rod 22. One end of the extrusion rod 23 is hinged with a roller 24. When the pull rod 22 moves, the pull rod 22 will drive the extrusion rod 23 to move, so that the subsequent extrusion rod 23 can drive the roller 24 to move.

[0030] Working principle of the utility model: One end of the pipeline body 18 with the positioning groove 19 enters the inside of the pipeline groove 13. At this time, one end of the connecting pipe 11 is inserted into the connecting hole 10. The soft rubber material inside the connecting hole 10 fixes the connecting pipe 11 inside the connecting hole 10. At this time, the pull rod 22 is pulled, causing the pull rod 22 to drive the extrusion rod 23 to move, and the extrusion rod 23 to drive the roller 24 to move. The outer wall of the roller 24 contacts the inclined surface on one side of the outer wall of the pressure receiving plate 21, causing the extrusion rod 23 to drive the pressure receiving plate 21 to slide inside the groove 14 through the roller 24. The pressure receiving plate 21 drives the moving plate 16 to move, causing the moving plate 16 to slide on the outer wall of the fixed rod 15 and inside the groove 14. The moving plate 16 squeezes the spring 20. At the same time, the moving plate 16 drives the positioning rod 17 to move, enabling one end of the positioning rod 17 to move from the inside of the pipeline groove 13 to the inside of the groove 14. At this time, the end of the pipeline body 18 with the positioning groove 19 completely enters the inside of the pipeline groove 13. When the positioning groove 19 is located on one side of the positioning rod 17, the pull rod 22 is released. The elastic force of the spring 20 drives the moving plate 16 and the positioning rod 17 back to the initial position. One end of the positioning rod 17 enters the inside of the pipeline groove 13, and one end of the positioning rod 17 enters the positioning groove 19, making the subsequent connection and disassembly of the pipeline body 18 and the convection chromatography column body 1 convenient and fast.

[0031] Finally, it should be noted that the above are only the preferred embodiments of the present utility model and are not used to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included in the protection scope of the present utility model.

Claims

1. A radial flow convection chromatography column, comprising a convection chromatography column body (1), characterized in that: The convection chromatography column body (1) is provided with a first filter groove (2) at the bottom end, a second filter groove (3) is provided inside the first filter groove (2), a gear block (4) is fixedly connected to the connection between the first filter groove (2) and the second filter groove (3), a filling piece (5) is provided inside the second filter groove (3), a bottom cover (6) is provided at the bottom end of the convection chromatography column body (1), a connecting groove (7) is provided at the bottom end of the bottom cover (6), a mounting groove (8) is provided at the bottom end of the convection chromatography column body (1) corresponding to the connecting groove (7), a threaded bolt (9) is provided inside the connecting groove (7), and one end of the threaded bolt (9) is connected to the mounting groove (8) by a thread.

2. The radial flow convection chromatography column according to claim 1, characterized in that: The top of the convection chromatography column body (1) and the bottom of the bottom cover (6) are both provided with a connection hole (10), and the connection hole (10) is connected to the second filter tank (3), and a connection pipe (11) is slidably connected inside the connection hole (10).

3. The radial flow convection chromatography column according to claim 2, characterized in that: The end of the connecting pipe (11) away from the connecting hole (10) is fixedly connected to a mounting plate (12); a pipe groove (13) is provided on the outer wall of the mounting plate (12) away from the connecting pipe (11); and a groove (14) is provided inside the mounting plate (12) on one side of the pipe groove (13).

4. The radial flow convection chromatography column according to claim 3, characterized in that: A fixing rod (15) is fixedly connected inside the groove (14), a movable plate (16) is slidably connected to the outer wall of the fixing rod (15), a positioning rod (17) is fixedly connected to the outer wall of the movable plate (16), one end of the positioning rod (17) passes through the inside of the pipeline groove (13) and the positioning rod (17) is slidably connected to the mounting plate (12), a pipeline body (18) is slidably connected inside the pipeline groove (13), a positioning groove (19) is provided on the outer wall of the pipeline body (18) and one end of the positioning rod (17) is slidably connected to the positioning groove (19).

5. The radial flow convection chromatography column according to claim 4, characterized in that: The outer wall of the movable plate (16) is fixedly connected to a spring (20), and the outer wall of the movable plate (16) is fixedly connected to a pressure plate (21) on one side of the positioning rod (17).

6. The radial flow convection chromatography column according to claim 3, characterized in that: A pull rod (22) is provided inside the groove (14), an outer wall of the pull rod (22) is fixedly connected to an extrusion rod (23), and one end of the extrusion rod (23) is hinged to a roller (24).

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