Magnetic rod stirring sleeve assembly and nucleic acid extractor

Abstract

The utility model relates to the technical field of nucleic acid extraction, and particularly discloses a magnetic rod stirring sleeve assembly and a nucleic acid extractor. The magnetic rod stirring sleeve assembly comprises a magnetic rod and a magnetic rod base, a plurality of magnetic suction seats with magnetism are arranged on the magnetic bar base; the magnetic bar is connected to the magnetic suction seat in a magnetic suction manner; the magnetic attraction base is used for generating magnetic traction force to the magnetic bar so that the magnetic bar can be perpendicular to the magnetic bar base. According to the scheme, through magnetic attraction cooperation of the magnetic attraction base and the magnetic bar, the magnetic bar can be finely adjusted in the horizontal position so as to adapt to stirring sleeves of different sizes; meanwhile, in the process that the magnetic bar is arranged in the stirring sleeve and in the stirring process of the stirring sleeve, the magnetic attraction base can continuously provide magnetic traction force for the magnetic bar to ensure the perpendicularity of the magnetic bar, the deviation of the magnetic bar and the center position of the stirring sleeve is reduced, and therefore the effect of improving the nucleic acid extraction quality is achieved.

Description

Technical Field

[0001] This application relates to the technical field of nucleic acid extraction, and particularly relates to a magnetic rod stirring sleeve assembly and a nucleic acid extractor. Background Art

[0002] Nucleic acids (DNA and RNA) are one of the basic substances in life science research and are widely used in many fields such as clinical diagnosis, food safety detection, and environmental monitoring. When studying them, nucleic acid extraction technology is needed to extract pure nucleic acids from complex biological samples.

[0003] Currently, the commonly used nucleic acid extraction method is magnetic adsorption extraction, including processes such as lysis, adsorption, washing, and elution. The specific process is to first use a stirring sleeve to stir a biological sample so that nucleic acids are released from the biological sample and adsorbed by magnetic beads. After that, the magnetic rod in the stirring sleeve adsorbs the magnetic beads.

[0004] In actual application, due to reasons such as the slight difference in size between different stirring sleeves, the actual position of the magnetic rod after being inserted into the stirring sleeve will deviate from the center of the stirring sleeve, making the magnetic rod unable to be located at the center position of the stirring sleeve, resulting in uneven adsorption distribution of the magnetic rod to the magnetic beads and affecting the extraction quality. Utility Model Content

[0005] In view of this, the purpose of this application is to provide a magnetic rod stirring sleeve assembly and a nucleic acid extractor to solve the problem that the position of the magnetic rod deviates from the center of the stirring sleeve during nucleic acid extraction.

[0006] To achieve the above technical purpose, the first aspect of this application provides a magnetic rod stirring sleeve assembly, including: a magnetic rod and a magnetic rod base;

[0007] A number of magnetic adsorption seats with magnetism are arranged on the magnetic rod base;

[0008] The magnetic rod is magnetically connected to the magnetic adsorption seat;

[0009] The magnetic adsorption seat is used to generate a magnetic traction force on the magnetic rod so that the magnetic rod is perpendicular to the magnetic rod base.

[0010] Further, the end face of the magnetic adsorption seat connected to the magnetic rod is a smooth plane;

[0011] The end face of the magnetic rod connected to the magnetic adsorption seat is a smooth plane.

[0012] Further, it further includes: a stirring sleeve and a rotating motor;

[0013] An inner cavity for the magnetic rod to extend into is arranged in the stirring sleeve;

[0014] The rotating motor is used to drive the stirring sleeve to rotate for stirring.

[0015] Further, it further includes: a support base;

[0016] A number of rotatable connecting members are provided on the support base;

[0017] The rotating motor is arranged on the support base, and the rotating motor is in transmission connection with each of the connecting members;

[0018] The connecting member is used for detachably connecting the stirring sleeve;

[0019] When the connecting member connects the stirring sleeve, the rotating motor drives the stirring sleeve to rotate by driving the connecting member to rotate.

[0020] Further, it further includes: a magnetic rod driving member;

[0021] A through hole is provided in the middle of the connecting member;

[0022] The output end of the magnetic rod driving member is connected to the magnetic rod;

[0023] The magnetic rod driving member is used to drive the magnetic rod to move up and down, so that the magnetic rod passes through the through hole and enters the inner cavity of the stirring sleeve, or the magnetic rod leaves the inner cavity of the stirring sleeve.

[0024] Further, it further includes a vertical driving member;

[0025] The output end of the vertical driving member is connected to the support base and is used to drive the support base to move up and down.

[0026] Further, it further includes a nucleic acid extraction reagent strip;

[0027] Before use, the stirring sleeve is encapsulated in the nucleic acid extraction reagent strip.

[0028] Further, it further includes: a torque sensor;

[0029] The torque sensor is used to sense the friction force data between the stirring sleeve and the magnetic rod.

[0030] Further, it further includes: a controller;

[0031] The controller is electrically connected to the torque sensor and the rotating motor;

[0032] The controller is used to control the forward rotation or reverse rotation of the rotating motor and control the output power of the rotating motor according to the friction force data.

[0033] The second aspect of the present application provides a nucleic acid extractor, which includes a device main body, a horizontal driving member, and the magnetic bar stirring sleeve assembly described in any one of the above;

[0034] The magnetic bar stirring sleeve assembly is arranged on the device main body;

[0035] A placement table for placing a nucleic acid extraction reagent strip is arranged on the device main body;

[0036] The horizontal driving member is arranged on the device main body and is used to drive the nucleic acid extraction reagent strip to move horizontally.

[0037] It can be seen from the above technical solutions that the present application provides a magnetic bar stirring sleeve assembly and a nucleic acid extractor; among them, the magnetic bar stirring sleeve assembly includes: a magnetic bar and a magnetic bar base; a plurality of magnetic attraction seats with magnetism are arranged on the magnetic bar base; the magnetic bar is magnetically connected to the magnetic attraction seats; the magnetic attraction seats are used to generate a magnetic traction force on the magnetic bar so that the magnetic bar is perpendicular to the magnetic bar base.

[0038] In this solution, through the magnetic attraction cooperation between the magnetic attraction seats and the magnetic bar, the magnetic bar can be finely adjusted in the horizontal position to adapt to different sizes of stirring sleeves; at the same time, during the process of loading the magnetic bar into the stirring sleeve and during the stirring process of the stirring sleeve, the magnetic attraction seats can continuously provide a magnetic traction force to the magnetic bar to ensure the perpendicularity of the magnetic bar, reduce the deviation between the magnetic bar and the central position of the stirring sleeve, thereby achieving the effect of improving the quality of nucleic acid extraction. Description of the Drawings

[0039] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required to be used in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0040] Figure 1 It is a schematic diagram of the magnetic bar and the magnetic bar base of a magnetic bar stirring sleeve assembly provided by an embodiment of the present application;

[0041] Figure 2 It is a schematic diagram of the stirring sleeve and the connecting member of a magnetic bar stirring sleeve assembly provided by an embodiment of the present application;

[0042] Figure 3 It is a schematic diagram of the stirring sleeve driving component of a magnetic bar stirring sleeve assembly provided by an embodiment of the present application;

[0043] Figure 4 It is a perspective view of a nucleic acid extractor provided by an embodiment of the present application;

[0044] Figure 5The front view of a nucleic acid extractor provided by an embodiment of the present application;

[0045] In the figure: 10, magnetic bar; 20, magnetic bar base; 21, magnetic attraction seat; 30, stirring sleeve; 40, rotating motor; 50, support seat; 51, connecting piece; 52, gear; 53, through hole; 60, magnetic bar driving part; 70, vertical driving part; 100, device main body; 110, horizontal driving part; 200, nucleic acid extraction reagent strip. Specific embodiments

[0046] Next, the technical solutions of the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. Based on the embodiments in the specification of the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope claimed by the present application.

[0047] In the description of the embodiments of the present application, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the embodiments of the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the embodiments of the present application. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0048] In the description of the embodiments of the present application, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a replaceable connection, or an integral connection. It can be a mechanical connection or an electrical connection. It can be directly connected or indirectly connected through an intermediate medium. It can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present application can be understood according to specific situations.

[0049] In the first aspect of the embodiments of the present application, a magnetic bar stirring sleeve assembly is provided to solve the problem that the position of the magnetic bar and the center position of the stirring sleeve will deviate during the nucleic acid extraction process.

[0050] Please refer to Figure 1 And Figure 2 , the magnetic bar stirring sleeve assembly provided in this embodiment includes: a magnetic bar 10 and a magnetic bar base 20. A plurality of magnetic attraction seats 21 with magnetism are arranged on the magnetic bar base 20; the magnetic bar 10 is magnetically connected to the magnetic attraction seat 21.

[0051] In this embodiment, the magnetic attraction seat 21 can be a magnet, and the magnetic pole of the magnetic attraction seat 21 is opposite to that of the magnetic rod 10. Since the magnetic attraction seat 21 and the magnetic rod 10 are connected by magnetic attraction, in application, the magnetic rod 10 can move along the surface of the magnetic attraction seat 21 for fine adjustment of the position, so as to adapt to the slight dimensional differences of different stirring sleeves 30 due to manufacturing processes and other reasons, and ensure that the magnetic rod 10 is aligned with the central axis of the stirring sleeve 30 before being inserted into the stirring sleeve 30.

[0052] Moreover, the magnetic attraction seat 21 can continuously provide a magnetic traction force to the magnetic rod 10, making the magnetic rod 10 perpendicular to the magnetic rod base 20, and ensuring that the magnetic rod 10 always maintains a vertical and centered state when being inserted into the stirring sleeve 30, avoiding the occurrence of perpendicularity deviation during the installation of the magnetic rod 10.

[0053] Meanwhile, in this embodiment, the magnetic attraction cooperation between the magnetic attraction seat 21 and the magnetic rod 10 can simplify the processes of adjusting the perpendicularity and horizontal position of the magnetic rod 10, achieving the effect of convenient adjustment.

[0054] As an implementation manner, the end face of the magnetic attraction seat 21 connected to the magnetic rod 10 is a smooth plane; the end face of the magnetic rod 10 connected to the magnetic attraction seat 21 is a smooth plane, ensuring that the magnetic rod 10 and the magnetic attraction seat 21 can be flat and perpendicular to each other for fitting.

[0055] In other implementation manners, the two end faces of the magnetic attraction seat 21 and the magnetic rod 10 connected to each other can be curved surfaces with mutually adapted shapes. For example, the magnetic attraction seat 21 is a convex surface while the magnetic rod 10 is a concave surface, as long as they can be mutually fitted after abutting against each other.

[0056] In other embodiments provided by the present application, please refer to Figures 1 to 3 , the magnetic rod stirring sleeve assembly further includes: a stirring sleeve 30 and a rotating motor 40; an inner cavity for the magnetic rod 10 to extend into is provided in the stirring sleeve 30; the rotating motor 40 is used to drive the stirring sleeve 30 to rotate for stirring.

[0057] That is to say, in this embodiment, the stirring sleeve 30 can be driven by the rotating motor 40 to rotate, and the biological sample is stirred in a rotating manner. Among them, as a rotating part, the stirring sleeve 30 can be arranged on a support structure, such as being arranged on the following support seat 50; the output end of the rotating motor 40 can be directly connected to the stirring sleeve 30, or can be connected to the stirring sleeve 30 through other connecting components, as long as the rotating motor 40 can drive the stirring sleeve 30 to rotate.

[0058] In practical applications, existing nucleic acid extractors generally stir in an up-and-down stirring manner, that is, the biological sample is stirred by driving the stirring sleeve 30 to move up and down. In this way, liquid splashing is likely to occur, resulting in a decrease in the magnetic bead recovery rate and cross-contamination between extraction products.

[0059] Therefore, in this embodiment, the stirring sleeve 30 can improve the mixing efficiency, reduce the cross-contamination rate between extraction holes, and ensure the length and integrity of nucleic acid fragments in the extraction product by rotating for stirring.

[0060] Optionally, the rotating motor 40 is arranged on the support base 50; a plurality of rotatable connecting members 51 are arranged on the support base 50; the rotating motor 40 is in transmission connection with each connecting member 51; the connecting member 51 is used for detachably connecting the stirring sleeve 30; when the connecting member 51 is connected to the stirring sleeve 30, the rotating motor 40 drives the stirring sleeve 30 to rotate by driving the connecting member 51 to rotate.

[0061] During the nucleic acid extraction process, the stirring sleeve 30 can be placed at the corresponding hole positions on the deep well plate. After manually or by a manipulator taking out the stirring sleeve 30 and installing it on the connecting member 51, the detachable connection between the stirring sleeve 30 and the connecting member 51 is realized. After that, when the rotating motor 40 is started, it can drive the connecting member 51 and the stirring sleeve 30 to rotate for stirring. After the nucleic acid extraction is completed, the stirring sleeve 30 can be taken off from the connecting member 51 and then discarded.

[0062] As an implementation manner, a gear 52 can be arranged on the connecting member 51; the output end of the rotating motor 40 is meshed with the gear 52, so that the rotating motor 40 can drive the connecting member 51 to rotate after being started.

[0063] In this embodiment, a plurality of magnetic seat 21 are arranged on the magnetic rod base 20; the plurality of magnetic seat 21 are arranged in a straight line; the plurality of magnetic seat 21 can respectively connect a plurality of magnetic rods 10 in one-to-one correspondence, and after connection, the plurality of magnetic rods 10 are also arranged in a straight line. Correspondingly, a plurality of connecting members 51 are arranged on the support base 50 and the plurality of connecting members 51 are arranged in a straight line. The gears 52 on the plurality of connecting members 51 are sequentially meshed with each other; the output end of the rotating motor 40 only needs to be meshed with any one of the connecting members 51, and it can drive the plurality of connecting members 51 to rotate simultaneously when starting. The plurality of connecting members 51 can respectively connect a plurality of stirring sleeves 30 in one-to-one correspondence, thereby improving the efficiency of nucleic acid extraction.

[0064] It should be noted that when the stirring sleeve 30 is placed on the deep well plate, there may be a situation of missing placement or insufficient verticality of the placement of the stirring sleeve 30, making it difficult for the connecting member 51 to accurately and effectively connect the stirring sleeve 30.

[0065] Therefore, in a further improved embodiment, please refer to Figure 4 , the magnetic rod stirring sleeve assembly further includes a nucleic acid extraction reagent strip 200; before use, the stirring sleeve 30 is encapsulated in the nucleic acid extraction reagent strip 200.

[0066] The nucleic acid extraction reagent strip 200 provided in this embodiment has multiple independent cavities; the spacing between adjacent cavities is greater than the hole spacing in the existing deep well plate, which can effectively prevent cross-contamination between different samples and improve the accuracy and reliability of nucleic acid extraction. The stirring sleeve 30 is encapsulated in the nucleic acid extraction reagent strip 200 before use, which can avoid the risk of missing placement of the stirring sleeve. At the same time, the cavity of the nucleic acid extraction reagent strip 200 has a higher self-verticality, which can allow the stirring sleeve 30 to be placed vertically, helping the connecting piece 51 to connect the stirring sleeve 30.

[0067] In another embodiment, please refer to Figure 5 , and it further includes: a magnetic rod driving member 60; a through hole 53 is provided in the middle of the connecting piece 51; the output end of the magnetic rod driving member 60 is connected to the magnetic rod 10; the magnetic rod driving member 60 is used to drive the magnetic rod 10 to move up and down, so that the magnetic rod 10 passes through the through hole and enters the inner cavity of the stirring sleeve 30, or the magnetic rod 10 leaves the inner cavity of the stirring sleeve 30.

[0068] Specifically, the connecting piece 51 is in the shape of a sleeve, which can allow the magnetic rod 10 to pass through the through hole 53 and can penetrate into the stirring sleeve 30 to grab the stirring sleeve 30.

[0069] The output end of the magnetic rod driving member 60 can be connected to the magnetic rod base 20, so as to connect the magnetic rod 10 through the magnetic rod base 20 and can drive the magnetic rod base 20 and the magnetic rod 10 to move up and down synchronously. The magnetic rod 10 can be arranged directly above the vertical direction of the connecting piece 51, that is, the central axis of the magnetic rod 10 coincides with the central axis of the through hole 53. When the magnetic rod 10 needs to adsorb magnetic beads, the magnetic rod driving member 60 can drive the magnetic rod 10 to descend, so that the magnetic rod 10 passes through the through hole 53 and enters the inner cavity of the stirring sleeve 30 to adsorb the magnetic beads.

[0070] Optionally, the magnetic rod stirring sleeve assembly further includes a vertical driving member 70; the output end of the vertical driving member 70 is connected to the support seat 50, and is used to drive the support seat 50 to move up and down, so as to drive the stirring sleeve 30 to enter or leave the biological sample.

[0071] Through the cooperation of the above-mentioned magnetic rod driving member 60, vertical driving member 70 and the rotating motor 40, the magnetic rod stirring sleeve assembly provided in this embodiment can form a structure for automatically extracting nucleic acids, reducing the working intensity of the staff.

[0072] In practical applications, when the stirring sleeve 30 rotates, the magnetic rod 10 will inevitably deviate from the center of the stirring sleeve 30, which may cause friction between the magnetic rod 10 and the inner wall of the stirring sleeve 30, affecting the normal operation of the machine. Seriously, it may cause wear of the magnetic rod 10 and detachment of the stirring sleeve 30.

[0073] For this reason, in an embodiment provided in the present application, it further includes: a torque sensor; the torque sensor is used to sense the friction force data between the stirring sleeve 30 and the magnetic rod 10.

[0074] The friction force data between the stirring sleeve 30 and the magnetic bar 10 can be monitored in real time through the torque sensor, which is convenient for the staff to judge the deviation of the magnetic bar 10 from the center of the stirring sleeve 30 in real time.

[0075] As a further improvement, the magnetic bar stirring sleeve assembly further includes: a controller; the controller is electrically connected to the torque sensor and the rotating motor 40; the controller is used to control the forward or reverse rotation of the rotating motor 40 and the output power of the rotating motor 40 according to the friction force data.

[0076] That is, in this embodiment, the controller can adjust the output speed and rotation direction of the rotating motor 40 according to the data detected by the torque sensor to correct the relative position between the magnetic bar 10 and the stirring sleeve 30.

[0077] Specifically, the process of automatic correction by the controller and the torque sensor can be as follows:

[0078] In the first step, the vertical driving member 70 drives the support seat 50 to descend, so that the connecting member 51 grabs the stirring sleeve 30 in the first cavity of the nucleic acid extraction reagent strip 200, and then the vertical driving member 70 drives the connecting member 51 and the stirring sleeve 30 to rise to the zero position. Among them, the zero position is the preset starting position of the stirring sleeve 30.

[0079] In the second step, the controller reads the torque of the stirring sleeve 30 during rotation before the magnetic bar 10 is inserted into the stirring sleeve 30 through the torque sensor as the background curve, and after smoothing the background curve, , for Take the average value to obtain the background torque value .

[0080] The smoothing processing algorithm is as follows:

[0081]

[0082] Where is the smoothed torque value, x j is the original torque value, and n is the window size.

[0083] In the third step, the magnetic bar driving member 60 drives the magnetic bar 10 to extend into the stirring sleeve 30, and during the process of the stirring sleeve 3, it notifies the torque sensor to continuously monitor the friction force between the magnetic bar 10 and the stirring sleeve 30. Any torque change deviating from the preset range will trigger the correction program. Taking the preset torque threshold as 0.1 N·m as an example, the torque threshold can be set to 0.1 N·m first.

[0084] When the magnetic bar 10 is inserted into the stirring sleeve 30, the controller reads the real-time data of the torque sensor during the rotation of the stirring sleeve 30. And background torque deduction is performed (subtracting the background torque value from the collected torque value).

[0085] If the torque value in the friction force data exceeds 0.1 N·m, it indicates that there is friction and the magnetic bar is not centered.

[0086] Correction process:

[0087] When the torque value is greater than 0.1 N·m, the rotating motor will rotate in the positive or negative direction at a speed of 100 revolutions per minute for no more than 5 seconds.

[0088] When the torque value is less than 0.1 N·m, the rotating motor will rotate in the positive or negative direction at a speed of 100 revolutions per minute for no more than 5 seconds.

[0089] This process will continue until the torque value is stabilized within 0.1 N·m, indicating that the magnetic bar 10 is centered.

[0090] Please refer to Figure 4 and Figure 5 , the second aspect of the present application provides a nucleic acid extractor, including a device main body 100, a horizontal driving member 110 and the magnetic bar stirring sleeve assembly of any one of the above; the magnetic bar stirring sleeve assembly is arranged on the device main body 100; a placement table for placing the nucleic acid extraction reagent strip 200 is arranged on the device main body 100; the horizontal driving member 110 is arranged on the device main body 100 and is used to drive the nucleic acid extraction reagent strip to move horizontally.

[0091] In this embodiment, the magnetic bar stirring sleeve assembly may include the above-mentioned rotating motor 40, magnetic bar driving member 60 and vertical driving member 70; wherein, the magnetic bar driving member 60 and the vertical driving member 70 may be linear driving members such as air cylinders, or may be linear motors or rotating motors. The horizontal driving member 110 may be a rotating motor.

[0092] The nucleic acid extraction process of the nucleic acid extractor provided in this embodiment is as follows:

[0093] Lysis process:

[0094] 11. The nucleic acid extraction reagent strip 200 is placed on the placement table, and then the horizontal driving member 110 drives the nucleic acid extraction reagent strip 200 to move to the grasping station. At this time, the first cavity of the nucleic acid extraction reagent strip 200 is located directly below the connecting member 51;

[0095] 12. The vertical driving member 70 drives the support seat 50 to descend, so that the connecting member 51 grabs the stirring sleeve 30 in the first cavity of the nucleic acid extraction reagent strip 200. Then, the vertical driving member 70 drives the connecting member 51 and the stirring sleeve 30 to rise to the zero position;

[0096] 13. The horizontal driving member 110 drives the nucleic acid extraction reagent strip 200 to move to the lysis station. At this time, the cavity containing the biological sample on the nucleic acid extraction reagent strip 200 is located directly below the stirring sleeve 30;

[0097] 14. The vertical driving member 70 drives the support base 50, the connecting member 51 and the stirring sleeve 30 to descend, so that the stirring sleeve 30 extends into the bottom of the cavity containing the lysis solution, magnetic beads and sample. The distance between the stirring sleeve 30 and the bottom of the cavity can be controlled to be 0.5 mm;

[0098] 15. The rotating motor 40 drives the stirring sleeve 30 to rotate. At an appropriate temperature, the sample and the lysis solution are mixed to release the nucleic acid substances in the sample cells;

[0099] 16. The magnetic rod driving member 60 drives the magnetic rod 10 to descend, so that the magnetic rod 10 passes through the through hole 53 and reaches the bottom of the stirring sleeve 30 for magnetic adsorption, and the magnetic beads adsorbed with nucleic acid substances are gathered to the bottom outside the stirring sleeve 30;

[0100] 17. After the magnetic adsorption is completed, the magnetic rod driving member 60 drives the magnetic rod 10 to rise, and the vertical driving member 70 drives the stirring sleeve 30 to rise, so that the magnetic rod 10 and the stirring sleeve 30 rise simultaneously at the same speed until the stirring sleeve 30 disengages from the lysis cavity of the nucleic acid extraction reagent strip 200.

[0101] Washing process:

[0102] 21. The horizontal driving member 110 drives the nucleic acid extraction reagent strip 200 to move to the washing station. At this time, the cavity storing the washing solution on the nucleic acid extraction reagent strip 200 is located directly below the stirring sleeve 30;

[0103] 22. The vertical driving member 70 and the magnetic rod driving member 60 are started simultaneously to drive the magnetic rod 10 and the stirring sleeve 30 to descend simultaneously at the same speed, so that the stirring sleeve 30 and the magnetic rod 10 penetrate into the bottom of the cavity containing the washing solution, and the distance between the stirring sleeve 30 and the bottom of the hole is 0.5 mm;

[0104] 23. The magnetic rod driving member 60 drives the magnetic rod 10 to rise, so that the magnetic beads adsorbed with nucleic acid are released into the washing solution;

[0105] 24. The rotating motor 40 drives the stirring sleeve 30 to rotate, mixes the washing solution, and washes the non-specific adsorbents on the magnetic beads;

[0106] 25. The magnetic rod driving member 60 drives the magnetic rod 10 to descend again, so that the magnetic beads are adsorbed again to the bottom outside the stirring sleeve 30;

[0107] 26. After the washing is completed, the vertical driving member 70 and the magnetic rod driving member 60 are started simultaneously to drive the magnetic rod 10 and the stirring sleeve 30 to rise simultaneously at the same speed, so that the stirring sleeve 30 is separated from the washing cavity of the nucleic acid extraction reagent strip 200.

[0108] Elution process:

[0109] 31. The horizontal driving member 110 drives the nucleic acid extraction reagent strip 200 to the elution station. The vertical driving member 70 and the magnetic rod driving member 60 are started simultaneously to drive the magnetic rod 10 and the stirring sleeve 30 to descend simultaneously at the same speed, so that the stirring sleeve 30 penetrates into the cavity filled with the eluent.

[0110] 32. The magnetic rod driving member 60 drives the magnetic rod 10 to rise, so that the magnetic beads are released into the elution solution.

[0111] 33. The rotating motor 40 drives the stirring sleeve 30 to rotate, mixes the elution solution, and releases the nucleic acid on the magnetic beads into the solution.

[0112] 34. After the elution is completed, the stirring sleeve 30 descends to the bottom of the hole. At the same time, the magnetic rod driving member 60 drives the magnetic rod 10 to descend to the bottom of the stirring sleeve 30. After the action of adsorbing the magnetic beads is completed, the vertical driving member 70 and the magnetic rod driving member 60 are started simultaneously to drive the magnetic rod 10 and the stirring sleeve 30 to rise simultaneously at the same speed, so that the stirring sleeve 30 is separated from the elution hole of the nucleic acid extraction reagent strip 200.

[0113] Magnetic bead recovery process:

[0114] 41. The horizontal driving member 110 drives the nucleic acid extraction reagent strip 200 to the magnetic bead recovery station. The vertical driving member 70 and the magnetic rod driving member 60 are started simultaneously to drive the magnetic rod 10 and the stirring sleeve 30 to descend simultaneously at the same speed, and the stirring sleeve 30 penetrates into the bottom of the cavity.

[0115] 42. The magnetic rod driving member 60 drives the magnetic rod 10 to rise, so that the magnetic beads are released in the recovery cavity.

[0116] 43. After the magnetic bead recovery is completed, the vertical driving member 70 and the magnetic rod driving member 60 are started simultaneously to drive the magnetic rod 10 and the stirring sleeve 30 to rise simultaneously at the same speed, so that the stirring sleeve 30 is separated from the nucleic acid extraction reagent strip 200.

[0117] Stirring sleeve discard:

[0118] 51. The horizontal driving member 110 drives the nucleic acid extraction reagent strip 200 to the stirring sleeve 30 discard station. The vertical driving member 70 drives the stirring sleeve 30 to descend into the hole. The stirring sleeve removing mechanism acts to complete the discarding action of the stirring sleeve 30, and the discarded stirring sleeve 30 enters the stirring sleeve discard cavity of the nucleic acid extraction reagent strip 200.

[0119] In an application, the stirring sleeve discard cavity can be the first cavity of the above-mentioned nucleic acid extraction reagent strip 200.

[0120] The above are the preferred embodiments of the present application and are not used to limit the present utility model. Although the present application has been described in detail with reference to the examples, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing examples, or perform equivalent replacements on some of the technical features. However, any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A magnetic rod stirring sleeve assembly, characterized in that: include: A magnetic bar (10) and a magnetic bar base (20); The magnetic rod base (20) is provided with a plurality of magnetic suction seats (21) having magnetic properties; The magnetic rod (10) is magnetically connected to the magnetic seat (21); The magnetic suction seat (21) is used to generate a magnetic traction force on the magnetic bar (10), so that the magnetic bar (10) is perpendicular to the magnetic bar base (20).

2. The magnetic rod stirring sleeve assembly according to claim 1, characterized in that: The end surface where the magnetic seat (21) is connected to the magnetic rod (10) is a smooth plane; The end surface where the magnetic rod (10) is connected to the magnetic seat (21) is a smooth plane.

3. The magnetic rod stirring sleeve assembly according to claim 1, characterized in that: Also includes: A stirring sleeve (30) and a rotating motor (40); The stirring sleeve (30) is provided with an inner cavity into which the magnetic rod (10) extends; The rotating motor (40) is used to drive the stirring sleeve (30) to rotate to perform stirring.

4. The magnetic rod stirring sleeve assembly according to claim 3, characterized in that: Also includes: Support seat (50); The support seat (50) is provided with a plurality of rotatable connecting members (51); The rotating motor (40) is arranged on the supporting seat (50), and the rotating motor (40) is drivingly connected to each of the connecting members (51); The connecting piece (51) is used for detachably connecting the stirring sleeve (30); When the connecting member (51) is connected to the stirring sleeve (30), the rotating motor (40) drives the connecting member (51) to rotate, thereby driving the stirring sleeve (30) to rotate.

5. The magnetic rod stirring sleeve assembly according to claim 4, characterized in that: Also includes: A magnetic rod driving member (60); A through hole (53) is provided in the middle of the connecting piece (51); The output end of the magnetic bar driving member (60) is connected to the magnetic bar (10); The magnetic rod driving member (60) is used to drive the magnetic rod (10) to rise and fall, so that the magnetic rod (10) passes through the through hole (53) and enters the inner cavity of the stirring sleeve (30), or the magnetic rod (10) leaves the inner cavity of the stirring sleeve (30).

6. The magnetic rod stirring sleeve assembly according to claim 4 or 5, characterized in that: Also includes a vertical drive member (70); The output end of the vertical driving member (70) is connected to the support base (50) and is used to drive the support base (50) to rise and fall.

7. The magnetic rod stirring sleeve assembly according to claim 3, characterized in that: Also included is a nucleic acid extraction reagent strip (200); The stirring sleeve (30) is encapsulated in the nucleic acid extraction reagent strip (200) before use.

8. The magnetic rod stirring sleeve assembly according to claim 3, characterized in that: Also includes: Torque sensor; The torque sensor is used to sense friction data between the stirring sleeve (30) and the magnetic bar (10).

9. The magnetic rod stirring sleeve assembly according to claim 8, characterized in that: Also includes: Controller; The controller is electrically connected to the torque sensor and the rotating motor (40); The controller is used to control the forward rotation or reverse rotation of the rotating motor (40) and to control the output power of the rotating motor (40) according to the friction force data.

10. A nucleic acid extraction instrument, characterized in that: It comprises a device body (100), a horizontal driving member (110), and a magnetic rod stirring sleeve assembly according to any one of claims 1 to 9; The magnetic rod stirring sleeve assembly is arranged on the device body (100); The device body (100) is provided with a placement table for placing the nucleic acid extraction reagent strip (200); The horizontal driving member (110) is arranged on the device body (100) and is used to drive the nucleic acid extraction reagent strip to move horizontally.

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