Plant stem cell exosome extracting and liquid supplementing mechanism

Abstract

The utility model discloses a plant stem cell exosome extraction liquid supplementing mechanism, which relates to the technical field of plant stem cell exosome extraction, and is characterized in that a motor rotates by a certain angle to drive a rotating roller to rotate, so that a connecting rod slides in a wave-shaped sliding chute, and a sliding rod is driven to drive a piston to lift; the highest points of the wave-shaped sliding groove are located on the same horizontal line, and the lowest points of the wave-shaped sliding groove are located on the same horizontal line, so that the motor rotates by the same angle, the lifting distance of the piston is the same, accurate control over the supplementing amount can be achieved, automatic operation is achieved, manual operation is not needed, and the working efficiency is improved. The liquid supplementing time is shortened, so that the working efficiency of extracting and supplementing the liquid for the exosome of the plant stem cells is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of plant stem cell exosome extraction, in particular to a liquid supplementing mechanism for plant stem cell exosome extraction. Background Technique

[0002] The greatest advantage of plant stem cell exosomes is that samples are easy to obtain and suitable for large-scale production. In addition, the physiological, chemical, and biological characteristics of plant stem cell exosomes determine their high stability, and they can accumulate in certain tissues in the body and circulate in peripheral blood for a long time. Combining their advantages such as low toxicity, low immunogenicity, and high cell uptake rate, they thus have great potential in disease treatment and the application development as nanocarriers.

[0003] In the existing plant stem cell exosome extraction, when ultrafiltration is used to extract exosomes, the sample needs to be centrifugally concentrated, and the waste liquid also needs to be discarded. Therefore, it is necessary to frequently supplement liquid into the ultrafiltration tube to meet the concentration requirements for concentration.

[0004] Currently, for liquid supplementation, the user needs to hold a liquid supplementer and squeeze out the sample liquid of plant stem cell exosomes by pressing to supplement it into the ultrafiltration tube. However, due to manual operation, precise control of the supplementation amount requires skilled operation to achieve, or it needs to be supplemented in multiple times, or after squeezing and supplementing, it is supplemented again in a dripping manner to achieve precise control of the supplementation amount, and the process is time-consuming. Content of the Utility Model

[0005] To solve the technical problems in the background technique, the utility model proposes a liquid supplementing mechanism for plant stem cell exosome extraction.

[0006] A liquid supplementing mechanism for plant stem cell exosome extraction proposed by the utility model includes a liquid outlet pipe. A piston is slidably connected inside the liquid outlet pipe. One side of the piston is provided with a sliding rod, and one end of the sliding rod extends to the outside of the liquid outlet pipe. It further includes a circulating lifting mechanism and a liquid supply mechanism. The circulating lifting mechanism is used to drive the sliding rod to lift or drive the sliding rod to circulate and lift, and the liquid supply mechanism is used to supply liquid to the liquid outlet pipe.

[0007] Preferably, both ends of the liquid outlet pipe are designed to be sealed. The penetration part of the sliding rod and the liquid outlet pipe is slidably connected, and a sealing ring is installed at the penetration part of the sliding rod and the liquid outlet pipe.

[0008] Preferably, the cyclic lifting mechanism includes a U-shaped frame, a rotating roller is rotatably connected inside the U-shaped frame, a motor is installed on one side of the U-shaped frame, the motor is used to drive the rotating roller to rotate, one end of the output shaft of the motor is installed on the rotating roller, a wavy chute is formed on the rotating roller, a fixing plate is installed inside the U-shaped frame, a strip-shaped groove is formed on the fixing plate, one side of the strip-shaped groove penetrates through the fixing plate, a connecting rod is slidably connected inside the strip-shaped groove, one end of the connecting rod extends into the wavy chute, and the other end of the connecting rod is installed on the sliding rod.

[0009] Preferably, the liquid outlet pipe is installed on the cyclic lifting mechanism.

[0010] Preferably, the liquid supply mechanism includes a liquid supply tank, and a liquid supply pipe is communicated between the liquid supply tank and the liquid outlet pipe, and a return pipe is communicated between the liquid supply tank and the liquid outlet pipe.

[0011] Preferably, one end of the liquid outlet pipe away from the sliding rod is communicated with a liquid outlet head.

[0012] Preferably, the multiple highest points of the wavy chute are located on the same horizontal line, and the multiple lowest points of the wavy chute are located on the same horizontal line

[0013] In the present utility model, the proposed extraction and liquid supplementing mechanism for plant stem cell exosomes has the following beneficial technical effects:

[0014] By rotating a certain angle of the motor to drive the rotating roller to rotate, the connecting rod slides in the wavy chute, thereby driving the sliding rod to drive the piston to lift. Since the multiple highest points of the wavy chute are located on the same horizontal line and the multiple lowest points of the wavy chute are located on the same horizontal line, when the motor rotates the same angle, the lifting distance of the piston is the same. Therefore, accurate control of the replenishment amount can be achieved, and it is carried out automatically without manual operation, shortening the liquid supplementing time, thereby improving the working efficiency of the extraction and liquid supplementing of plant stem cell exosomes.

[0015] The additional aspects and advantages of the present utility model will be partly given in the following description, partly will become obvious from the following description, or will be understood through the practice of the present utility model. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is the front view of the structure of the present utility model;

[0017] Figure 2 It is the schematic diagram of the structure of the cyclic lifting mechanism of the present utility model driving the sliding rod to rise.

[0018] In the figure, 1 is the liquid outlet pipe; 2 is the piston; 3 is the sliding rod; 4 is the liquid outlet head; 5 is the circulating lifting mechanism; 51 is the U-shaped frame; 52 is the rotating roller; 53 is the motor; 54 is the wavy chute; 55 is the fixed plate; 56 is the strip-shaped groove; 57 is the connecting rod; 6 is the liquid supply mechanism; 61 is the liquid supply tank; 62 is the liquid supply pipe; 63 is the return pipe. Detailed implementation mode

[0019] The embodiments of the present invention will be described in detail below. The examples of the embodiments are shown in the drawings, in which the same or similar symbols represent the same or similar elements or elements with the same or similar functions from beginning to end. The embodiments described below by referring to the drawings are exemplary and are only used to explain the present invention, and should not be construed as a limitation to the present invention.

[0020] As Figure 1 - Figure 2 shown, a plant stem cell exosome extraction and replenishing liquid mechanism includes a liquid outlet pipe 1. A piston 2 is slidably connected in the liquid outlet pipe 1. One side of the piston 2 is provided with a sliding rod 3. One end of the sliding rod 3 extends to the outside of the liquid outlet pipe 1. Both ends of the liquid outlet pipe 1 are designed to be sealed. The through part of the sliding rod 3 and the liquid outlet pipe 1 is slidably connected. The liquid outlet pipe 1, the piston 2, and the sliding rod 3 are all prior arts. The end of the liquid outlet pipe 1 away from the sliding rod 3 is communicated with a liquid outlet head 4. The liquid outlet head 4 is the nipple of a syringe in the prior art. Only when pressure is applied to the piston 2 will the liquid be extruded from the nipple.

[0021] The further improvement of the technical solution lies in that it further includes a circulating lifting mechanism 5 and a liquid supply mechanism 6. The circulating lifting mechanism 5 is used to drive the sliding rod 3 to lift or drive the sliding rod 3 to circulate and lift, and the liquid supply mechanism 6 is used to supply liquid to the liquid outlet pipe 1.

[0022] In an optional embodiment, a sealing ring is installed at the through part of the sliding rod 3 and the liquid outlet pipe 1.

[0023] The further improvement of the technical solution lies in that the circulating lifting mechanism 5 includes a U-shaped frame 51. A rotating roller 52 is rotatably connected in the U-shaped frame 51. One side of the U-shaped frame 51 is provided with a motor 53. The motor 53 is used to drive the rotating roller 52 to rotate. One end of the output shaft of the motor 53 is installed on the rotating roller 52. A wavy chute 54 is provided on the rotating roller 52. A fixed plate 55 is installed in the U-shaped frame 51. A strip-shaped groove 56 is provided on the fixed plate 55. One side of the strip-shaped groove 56 penetrates through the fixed plate 55. A connecting rod 57 is slidably connected in the strip-shaped groove 56. One end of the connecting rod 57 extends into the wavy chute 54, and the other end of the connecting rod 57 is installed on the sliding rod 3.

[0024] In an optional embodiment, the multiple highest points of the wavy chute 54 are located on the same horizontal line, and the multiple lowest points of the wavy chute 54 are located on the same horizontal line.

[0025] In an alternative embodiment, the motor 53 is a stepper motor 53.

[0026] The motor 53 can be replaced with other existing drive structures that can drive the rotating roller 52 to rotate.

[0027] A further improvement of the technical solution lies in that the liquid outlet pipe 1 is installed on the circulating lifting mechanism 5.

[0028] In an alternative embodiment, the liquid outlet pipe 1 is installed on the U-shaped frame 51.

[0029] A further improvement of the technical solution lies in that the liquid supply mechanism 6 includes a liquid supply tank 61, and a liquid supply pipe 62 is connected between the liquid supply tank 61 and the liquid outlet pipe 1, and a return pipe 63 is connected between the liquid supply tank 61 and the liquid outlet pipe 1.

[0030] As Figure 2 shown, when the piston 2 is at the highest position, the liquid supply pipe 62 is located below the piston 2, and the return pipe 63 is located above the piston 2.

[0031] By rotating the motor 53 by a certain angle, the rotating roller 52 is driven to rotate, so that the connecting rod 57 slides in the wavy chute 54, thereby driving the sliding rod 3 to drive the piston 2 to lift and lower. Since the multiple highest points of the wavy chute 54 are on the same horizontal line and the multiple lowest points of the wavy chute 54 are on the same horizontal line, when the motor 53 rotates by the same angle, the lifting and lowering distance of the piston 2 is the same. Therefore, precise control of the replenishment amount can be achieved and it is carried out automatically without manual operation, shortening the liquid replenishment time, thereby improving the working efficiency of plant stem cell exosome extraction and liquid replenishment.

[0032] During the working process of this embodiment:

[0033] The liquid supply tank 61 flows the sample liquid of plant stem cell exosomes into the liquid outlet pipe 1 through the liquid supply pipe 62. By rotating the motor 53 by a certain angle, the rotating roller 52 is driven to rotate, so that the connecting rod 57 slides in the wavy chute 54, the connecting rod 57 rises and falls, thereby driving the sliding rod 3 to rise and fall, and further driving the piston 2 to rise and fall. Since the multiple highest points of the wavy chute 54 are on the same horizontal line and the multiple lowest points of the wavy chute 54 are on the same horizontal line, when the motor 53 rotates by the same angle, the lifting and lowering distance of the piston 2 is the same. Therefore, precise control of the replenishment amount can be achieved;

[0034] Rotating the motor 53 by a certain angle can cause the piston 2 to lift and lower cyclically, that is, the connecting rod 57 circulates between the highest point and the lowest point of the wavy chute 54, so that the amount of liquid discharged from the liquid outlet pipe 1 each time is fixed;

[0035] When the piston 2 is driven to descend, the residual liquid that may exist in the liquid supply pipe 62 flows into the space formed between the upper part of the piston 2 and the inner wall of the liquid outlet pipe 1. As the piston 2 ascends, this part of the residual liquid is driven by the piston 2 to ascend and can flow back into the liquid supply tank 61 through the return pipe 63, thus avoiding over-supply of liquid caused by the residual liquid in the liquid supply pipe 62.

[0036] Meanwhile, the content not described in detail in this specification belongs to the prior art well-known to those skilled in the art.

[0037] It should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0038] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of such features. In the description of the present invention, the meaning of "a plurality" is at least two, such as two, three, etc., unless otherwise specifically defined.

[0039] In the present invention, unless otherwise clearly specified and limited, the terms such as "installation", "connection", "connection", "fixation", etc. should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection, an electrical connection or communicable with each other; it may be directly connected, or indirectly connected through an intermediate medium, and may be the communication inside two elements or the interaction relationship between two elements, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0040] In the present utility model, unless otherwise clearly stipulated and defined, the first feature being "on" or "under" the second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "underneath" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply indicates that the first feature has a lower horizontal height than the second feature.

[0041] As mentioned above, the above is only a preferred specific embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present utility model, according to the technical solution and inventive concept of the present utility model, making equivalent replacements or changes, should be covered within the protection scope of the present utility model.

Claims

1. A plant stem cell exosome extraction and rehydration mechanism, comprising a liquid outlet pipe (1), a piston (2) slidably connected inside the liquid outlet pipe (1), a sliding rod (3) installed on one side of the piston (2), one end of the sliding rod (3) extending to the outside of the liquid outlet pipe (1), characterized in that: It also comprises a circulating lifting mechanism (5) and a liquid supply mechanism (6), wherein the circulating lifting mechanism (5) is used to drive the slide bar (3) to rise and fall or to drive the slide bar (3) to rise and fall in a circulating manner, and the liquid supply mechanism (6) is used to supply liquid to the liquid outlet pipe (1).

2. The plant stem cell exosome extraction and rehydration mechanism according to claim 1, characterized in that: Both ends of the liquid outlet pipe (1) are of sealed design, the penetration point of the sliding rod (3) and the liquid outlet pipe (1) is in sliding connection, and a sealing ring is installed at the penetration point of the sliding rod (3) and the liquid outlet pipe (1).

3. The plant stem cell exosome extraction and rehydration mechanism according to claim 1, characterized in that: The circulating lifting mechanism (5) comprises a U-shaped frame (51), wherein a rotating roller (52) is rotatably connected inside the U-shaped frame (51), a motor (53) is installed on one side of the U-shaped frame (51), and the motor (53) is used to drive the rotating roller (52) to rotate, and a wave-shaped sliding groove (54) is provided on the rotating roller (52), and a fixing plate (55) is installed inside the U-shaped frame (51), and a strip groove (56) is provided on the fixing plate (55), and one side of the strip groove (56) passes through the fixing plate (55), and a connecting rod (57) is slidably connected inside the strip groove (56), and one end of the connecting rod (57) extends into the wave-shaped sliding groove (54), and the other end of the connecting rod (57) is installed on the sliding rod (3).

4. The plant stem cell exosome extraction and rehydration mechanism according to claim 1, characterized in that: The liquid outlet pipe (1) is installed on the circulation lifting mechanism (5).

5. The plant stem cell exosome extraction and rehydration mechanism according to claim 1, characterized in that: The liquid supply mechanism (6) comprises a liquid supply box (61), a liquid supply pipe (62) is connected between the liquid supply box (61) and the liquid outlet pipe (1), and a reflux pipe (63) is connected between the liquid supply box (61) and the liquid outlet pipe (1).

6. The plant stem cell exosome extraction and rehydration mechanism according to claim 1, characterized in that: One end of the liquid outlet pipe (1) away from the slide rod (3) is connected to a liquid outlet head (4).

7. The plant stem cell exosome extraction and rehydration mechanism according to claim 3, characterized in that: The multiple highest points of the wave-shaped chute (54) are located on the same horizontal line, and the multiple lowest points of the wave-shaped chute (54) are located on the same horizontal line.

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