Exosome preservation device

Abstract

The utility model belongs to the field of biological cell preservation, and particularly relates to an exosome preservation device. According to the technology, a refrigerating box is included, a partition plate used for dividing the interior of the refrigerating box into an upper independent storage area and a lower independent storage area is fixed in the upper end of the refrigerating box, a plurality of containing grooves are fixed to the inner bottom of the refrigerating box, a storage device is placed on each containing groove, and a cooling assembly used for refrigerating the lower storage area is installed on the side wall of the refrigerating box; a control panel used for controlling the refrigerating temperature is installed on the front side wall of the refrigerating box, a plurality of through grooves used for the storages to penetrate through are formed in the bottom of the partition plate, and heat preservation covers used for sealing the through grooves are detachably installed at the positions, corresponding to the through grooves, of the top of the partition plate. According to the exosome storage device, the phenomenon that other exosomes are repeatedly frozen and thawed when part of the exosomes are stored and taken is avoided through the heat preservation covers in one-to-one correspondence with the storages, and sample loss caused by the fact that part of the exosomes are adhered to the inner wall of the storage pipe and cannot be poured out can be prevented.

Description

Technical Field

[0001] The utility model belongs to the field of biological cell preservation, and particularly relates to an exosome preservation device. Background Art

[0002] Mesenchymal stem cells are a type of pluripotent stem cells with a wide range of sources and can be obtained from tissues such as bone marrow, umbilical cord, and adipose tissue. They have three major mechanisms, namely multi-directional differentiation potential, paracrine effect, and homing effect, and are relatively common and widespread in clinical applications. The paracrine effect of mesenchymal stem cells is that they secrete a variety of paracrine factors, collectively referred to as secretome, to support the regeneration process of damaged tissues. The paracrine substances of mesenchymal stem cells include three major components: paracrine (less than 10nm), exosomes (40 - 100nm), and microvesicles (200 - 1000nm). Until exosomes were discovered, mesenchymal stem cell exosomes have also attracted extensive attention in the medical field. They not only have the general characteristics of exosomes, containing rich biological substances, but also have biological functions similar to their parental cells, playing biological roles similar to mesenchymal stem cells, including anti-inflammatory, antioxidant, promoting cell proliferation, and repairing damaged tissues, etc.

[0003] Currently, the preservation of exosomes is generally divided into cryopreservation for long-term storage and refrigerated preservation for short-term storage according to the storage time. Among them, the refrigerated preservation method requires strictly controlling the exosomes in an environment of 4 degrees Celsius to avoid the phenomenon of repeated freezing and thawing of exosomes due to temperature changes. However, traditional exosome preservation devices basically adopt the method of jointly accessing multiple test tubes. That is, during the process of accessing a certain test tube, not only will cold air leak out, but the test tubes will also be exposed to room temperature, resulting in the phenomenon of repeated freezing and thawing of unused exosomes. Summary of the Utility Model

[0004] The purpose of the utility model is to provide an exosome preservation device that can reduce the degree of temperature change inside the device when accessing exosomes.

[0005] The described exosome preservation device includes a refrigerated box with a box cover. A partition for dividing the interior of the refrigerated box into upper and lower two independent storage areas is fixedly installed inside the upper end of the refrigerated box. A plurality of placement grooves are fixedly installed at the inner bottom of the refrigerated box. A memory for preserving exosomes is placed on each placement groove. A cooling component for refrigerating the lower storage area is installed on the side wall of the refrigerated box. A control panel for controlling the refrigeration temperature is installed on the front side wall of the refrigerated box. A plurality of through grooves for the memory to pass through are opened at the bottom of the partition. A heat preservation cover for closing the through groove is detachably installed at the top of the partition corresponding to each through groove.

[0006] Further, the memory includes a storage tube vertically arranged with a liquid outlet opened at the lower end. A piston is arranged in the storage tube and is in sliding and sealing fit with the storage tube. A piston rod for pushing the piston to slide along the storage tube is fixed to the top of the piston. A protective sleeve in threaded fit with the storage tube and used to prevent accidental contact with the piston rod is sleeved on the upper end of the storage tube. A sealing cover in threaded fit with the storage tube is sleeved on the lower end of the storage tube. A sealing block for sealing the liquid outlet is fixed to the inner bottom of the sealing cover.

[0007] Further, annular grooves are opened at both the upper end and the lower end of the storage tube, and first annular plates are fixed in the annular grooves. Second annular plates in threaded fit with the corresponding first annular plates are fixed to the top of the sealing cover and the bottom of the protective sleeve.

[0008] Further, the cooling assembly includes an installation groove opened on the outer side wall of the refrigerator and communicating with the lower storage area. A refrigerator is installed in the installation groove. A heat conducting sheet in contact with the cold end of the refrigerator and used to close the installation groove is fixed to the inner wall of the lower storage area.

[0009] Further, a temperature sensor is installed on the inner wall of the lower storage area. The refrigerator and the temperature sensor are both electrically connected to the control panel.

[0010] Further, a protective net is installed at one end of the installation groove away from the heat conducting sheet.

[0011] Further, there are two groups of the cooling assemblies, which are respectively located in the left side wall and the right side wall of the refrigerator.

[0012] Further, a sponge pad is fixed in the placement groove, and the sealing cover is clamped in the sponge pad.

[0013] Further, a rubber ring for clamping the protective sleeve is fixed in the through groove.

[0014] Further, a battery for supplying power to the temperature sensor and the refrigerator is installed at the bottom of the refrigerator. Compared with the prior art, the utility model has the following beneficial effects:

[0015] The utility model enables relevant personnel to access a certain part of the memory by means of a number of heat preservation covers corresponding to the memories one by one. When accessing a certain part of the memory, only the corresponding heat preservation cover needs to be opened to expose the corresponding memory, so that all memories will not be exposed to the air. Compared with the traditional storage device, the amount of cold air overflow in this embodiment is small. Therefore, the temperature change degree in the lower storage area is low, and it can quickly return to 4 degrees Celsius under the action of the cooling component and the control panel, so as to avoid the phenomenon of repeated freezing and thawing of other exosomes when accessing some exosomes. The memory is composed of a storage tube, a protective sleeve, a piston rod, a piston, a sealing cover and a sealing block. The piston that is in sliding seal fit with the storage tube is pushed by the piston rod to move along the storage tube, so as to fully push out the exosomes in the storage tube from the liquid outlet, thus avoiding the loss of samples caused by some exosomes adhering to the inner wall of the storage tube and being unable to be poured out when the exosomes are naturally poured out. At the same time, after the air in the storage tube is discharged by using the piston, the liquid outlet can be sealed by the sealing cover and the sealing block, so as to achieve the effect of sealing and storing exosomes, thereby reducing the influence of air on exosomes and increasing the storage period. Description of the Drawings

[0016] Figure 1 is a schematic structural diagram of the utility model;

[0017] Figure 2 is Figure 1 the front side view of;

[0018] Figure 3 is Figure 1 the schematic structural diagram of the memory in;

[0019] Figure 4 is Figure 1 the top view of removing the box cover;

[0020] Names of each component in the figure: 1. Refrigerator box; 2. Protective net; 3. Heat preservation cover; 4. Memory; 4.1 Storage tube; 4.2 Protective sleeve; 4.3 Piston rod; 4.4 Piston; 4.5 Sealing cover; 4.6 Sealing block; 5. Partition board; 6. Refrigerator; 7. Heat conduction sheet; 8. Temperature sensor; 9. Sponge pad; 10. Battery; 11. Placing groove; 12. Control panel; 13. First annular plate; 14. Second annular plate. Detailed Embodiment

[0021] The following further illustrates the utility model through specific embodiments in conjunction with the drawings, but the utility model is not limited thereto. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the present invention.

[0022] Embodiment 1

[0023] An exosome preservation device according to this embodiment includes a refrigerated box 1 with a box cover. As Figure 1 and Figure 2 shown, the rear end of the box cover is hinged to the refrigerated box 1 through a hinge, and the front end of the box cover is fixedly connected to the refrigerated box 1 through a lock. Both the refrigerated box 1 and the box cover are made of metal;

[0024] A partition 5 for dividing the interior of the refrigerated box 1 into two independent upper and lower storage areas is fixedly installed inside the upper end of the refrigerated box 1. As Figure 1 shown, the partition 5 is horizontally arranged, and its side wall is fixed to the inner wall of the refrigerated box 1. The lower storage area is a refrigerated area and has a larger capacity than the upper storage area;

[0025] A number of placement slots 11 are fixedly installed at the inner bottom of the refrigerated box 1. A memory 4 for preserving exosomes is placed on each placement slot 11. As Figure 1 and Figure 3 shown, the memory 4 includes a storage tube 4.1 that is vertically arranged and has a liquid outlet at the lower end. A piston 4.4 that is in sliding and sealing fit with the storage tube 4.1 is provided inside the storage tube 4.1. A piston rod 4.3 for pushing the piston 4.4 to slide along the storage tube 4.1 is fixed to the top of the piston 4.4. A protective sleeve 4.2 that is in threaded fit with the storage tube 4.1 and is used to prevent accidental contact with the piston rod 4.3 is sleeved on the upper end of the storage tube 4.1. A sealing cover 4.5 that is in threaded fit with the storage tube 4.1 is sleeved on the lower end of the storage tube 4.1. A sealing block 4.6 for sealing the liquid outlet is fixed to the inner bottom of the sealing cover 4.5. Since exosomes are easily adsorbed on the inner wall of the container, resulting in sample loss, in this embodiment, the piston 4.4 that is in sliding and sealing fit with the storage tube 4.1 is pushed by the piston rod 4.3 to move along the storage tube 4.1, so as to fully push out the exosomes in the storage tube 4.1 from the liquid outlet, thereby avoiding sample loss caused by part of the exosomes adhering to the inner wall of the storage tube 4.1 and being unable to be poured out when the exosomes are naturally poured out. At the same time, after using the piston 4.4 to discharge the air in the storage tube 4.1, the sealing cover 4.5 and the sealing block 4.6 can seal the liquid outlet, so as to achieve the effect of sealing and preserving exosomes, thereby reducing the influence of air on exosomes and increasing the preservation period; the storage tube 4.1 is a transparent and closed tube at the lower end, and both the protective sleeve 4.2 and the sealing cover 4.5 are made of plastic;

[0026] As Figure 1 and Figure 2 shown, a sponge pad 9 is fixedly installed in the placement slot 11, and the sealing cover 4.5 is clamped in the sponge pad 9, so that the sponge pad 9 can not only limit the lower end of the memory 4, but also play a buffering role when the device collides;

[0027] In this embodiment, annular grooves are formed at both the upper and lower ends of the storage tube 4.1, and first annular plates 13 are fixed in the annular grooves. Second annular plates 14 that are in threaded fit with the corresponding first annular plates 13 are fixed to the top of the sealing cover 4.5 and the bottom of the protective sleeve 4.2 respectively. External threads are formed on both first annular plates 13, and the two second annular plates 14 are integrally formed with the protective sleeve 4.2 and the sealing cover 4.5 respectively and are provided with internal threads. An installation groove communicating with the lower storage area is formed on the outer side wall of the refrigerator 1, and a cooler 6 is installed in the installation groove. A heat conducting sheet 7 that contacts the cold end of the cooler 6 and is used to close the installation groove is fixed to the inner wall of the lower storage area (the cooler 6 and the heat conducting sheet 7 in this paragraph together form the cooling assembly for cooling the lower storage area, and the heat conducting sheet 7 can be a multi-sheet structure as shown in Figure 1 or a multi-columnar structure), in this embodiment, the cooler 6 is used to cool the lower storage area, and the heat conducting sheet 7 is used to accelerate the heat exchange rate, so that the lower storage area can quickly return to the temperature suitable for exosome preservation; in this embodiment, the cooler 6 is composed of a semiconductor refrigeration sheet and a radiator with a fan. The semiconductor refrigeration sheet is a prior art, and its principle is to utilize the Peltier effect of semiconductor materials. When direct current passes through an electric couple formed by two different semiconductor materials connected in series, heat can be absorbed and released at both ends of the electric couple respectively, so as to achieve refrigeration, as shown in Figure 1 . Among them, the installation groove communicates with a fixing groove having the same size as the semiconductor refrigeration sheet. The semiconductor refrigeration sheet is fixed in the fixing groove. The cold end of the semiconductor refrigeration sheet is fixed to the heat conducting sheet 7, and the hot end of the semiconductor refrigeration sheet is fixed to the radiator, and the fan of the radiator blows air outside the refrigerator 1;

[0028] Refer to Figure 1 and Figure 2 . A control panel 12 for controlling the refrigeration temperature is installed on the front side wall of the refrigerator 1. A temperature sensor 8 is installed on the inner wall of the lower storage area. Both the cooler 6 and the temperature sensor 8 are electrically connected to the control panel 12. In this embodiment, the temperature of the lower storage area is detected by the temperature sensor 8 and the cooler 6 is controlled by the control panel 12, so that the lower storage area is maintained at 4 degrees Celsius, thus ensuring that the exosomes stored in the short term are always at the optimal preservation temperature; as shown in Figure 2 . Among them, the control panel 12 is composed of a housing, a controller, a display screen and buttons. The display screen, buttons, temperature sensor 8, semiconductor refrigeration sheet and fan are all electrically connected to the controller through wires. As shown in Figure 1 , a battery 10 for supplying power to the electrical components in this embodiment is installed at the bottom of the refrigerator 1. This battery 10 is a storage battery;

[0029] Refer to Figure 1 and Figure 4, a plurality of through grooves for the memory 4 to pass through are provided at the bottom of the partition plate 5, and a heat preservation cover 3 for closing the through groove can be detachably installed at the top of the partition plate 5 corresponding to each through groove. In this embodiment, through a plurality of heat preservation covers 3 corresponding to the memory 4 one by one, when relevant personnel access a certain part of the memory 4, they only need to open the corresponding heat preservation cover 3 to expose the corresponding memory 4, so that all memories 4 will not be exposed to the air. And compared with the traditional storage device, the amount of cold air overflow in this embodiment is small, so the temperature change degree in the lower storage area is low, and it can quickly return to 4 degrees Celsius, so as to avoid the phenomenon of repeated freezing and thawing of other exosomes when accessing some exosomes; as Figure 1 and Figure 4 shown, a rubber ring for clamping the protective sleeve 4.2 is fixed in the through groove, so as to limit the upper end of the memory 4. A circular slot is provided at the top of the partition plate 5 corresponding to each heat preservation cover 3, and the lower end of the heat preservation cover 3 is inserted or threadedly engaged in the circular slot; wherein the heat preservation cover 3 is made of heat preservation material or the inner wall is covered with a heat preservation structure;

[0030] In the actual application of this embodiment, heat preservation layers are laid on the inner walls of the lower storage area (including the inner bottom of the refrigerating box 1 and the bottom of the partition plate 5), so as to further improve the heat preservation effect and avoid the influence of the external temperature on the temperature of the lower storage area.

[0031] When this embodiment is in use, first, to access the memory 4, open the lid of the refrigerating box 1, then unscrew the heat preservation cover 3 that you want to remove, and finally take out or insert the memory 4 and quickly screw on the heat preservation cover 3; second, to take out the exosomes in the memory 4, first unscrew the protective sleeve 4.2, then unscrew the sealing cover 4.5, and finally push the piston rod 4.3 to move the piston 4.4 to push out the exosomes in the storage tube 4.1.

[0032] Embodiment 2

[0033] This embodiment further illustrates the technology. A protective net 2 is installed at one end of the installation groove away from the cold guide sheet 7. As Figure 1 shown, the protective net 2 can prevent sundries or the limbs of relevant personnel from contacting the fan of the cooler 6, and the protective net 2 is detachably installed in the installation groove at one end away from the cold guide sheet 7 by means of screws or the like.

[0034] Embodiment 3

[0035] This embodiment further illustrates the technology. There are two groups of cooling components, which are respectively located in the left side wall and the right side wall of the refrigerating box 1, and the two groups of cooling components are symmetrically distributed left and right. As Figure 1 shown, this embodiment improves the refrigeration efficiency through two groups of cooling components, so that the device can further quickly return to the optimal preservation temperature of 4 degrees Celsius for exosomes after accessing the memory 4.

Claims

1. An exosome storage device, comprising a refrigerator (1) with a lid, characterized in that: A partition (5) for dividing the interior of the refrigerator (1) into two independent storage areas, an upper and a lower area, is fixed at the upper end of the refrigerator (1); a plurality of placement grooves (11) are fixed at the inner bottom of the refrigerator (1); a storage device (4) for storing exosomes is placed on each placement groove (11); a cooling component for cooling the storage area below is installed on the side wall of the refrigerator (1); a control panel (12) for controlling the refrigeration temperature is installed on the front side wall of the refrigerator (1); a plurality of through grooves for the storage device (4) to pass through are opened at the bottom of the partition (5); and a heat preservation cover (3) for closing the through groove is detachably installed on the top of the partition (5) corresponding to each through groove.

2. The exosome storage device according to claim 1, characterized in that: The storage device (4) comprises a storage tube (4.1) which is arranged vertically and has a liquid outlet at the lower end. A piston (4.4) is arranged in the storage tube (4.1) and is in sliding sealing engagement with the storage tube. A piston rod (4.3) is fixed to the top of the piston (4.4) and is used to push the piston (4.4) to slide along the storage tube (4.1). A protective sleeve (4.2) is sleeved on the upper end of the storage tube (4.1) and is in threaded engagement with the storage tube (4.1) and is used to prevent the piston rod (4.3) from being accidentally touched. A sealing cover (4.5) is sleeved on the lower end of the storage tube (4.1) and is in threaded engagement with the storage tube (4.1). A sealing block (4.6) is fixed to the inner bottom of the sealing cover (4.5) and is used to seal the liquid outlet.

3. The exosome storage device according to claim 2, characterized in that: The upper and lower ends of the storage tube (4.1) are both provided with annular grooves, and a first annular plate (13) is fixed in each of the annular grooves. The top of the sealing cover (4.5) and the bottom of the protective sleeve (4.2) are both fixed with a second annular plate (14) threadedly matched with the corresponding first annular plate (13).

4. The exosome storage device according to claim 1 or 2, characterized in that: The cooling assembly comprises a mounting groove provided on the outer wall of the cold storage box (1) and communicating with the storage area below, a refrigerator (6) being installed in the mounting groove, and a cooling plate (7) contacting the cold end of the refrigerator (6) and used to close the mounting groove being fixed on the inner wall of the storage area below.

5. The exosome storage device according to claim 4, characterized in that: A temperature sensor (8) is installed on the inner wall of the lower storage area, and the refrigerator (6) and the temperature sensor (8) are both electrically connected to the control panel (12).

6. The exosome storage device according to claim 4, characterized in that: A protective net (2) is installed at one end of the installation groove away from the cooling fin (7).

7. The exosome storage device according to claim 6, characterized in that: There are two groups of cooling components, which are respectively located in the left wall and the right wall of the cold storage box (1).

8. The exosome storage device according to claim 2, characterized in that: A sponge pad (9) is fixed in the placement groove (11), and the sealing cover (4.5) is clamped in the sponge pad (9).

9. The exosome storage device according to claim 8, characterized in that: A rubber ring for clamping the protective sleeve (4.2) is fixed in the through groove.

10. The exosome storage device according to claim 5, characterized in that: A battery (10) for supplying power to a temperature sensor (8) and a refrigerator (6) is installed at the bottom of the refrigerator (1).

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