Single-mode extraction module and nucleic acid extraction equipment

Abstract

The utility model provides a single-mode extraction module and nucleic acid extraction equipment, and relates to a nucleic acid extraction device, the single-mode extraction module comprises a magnetic attraction mechanism, the magnetic attraction mechanism comprises a support body, and the support body is provided with an accommodating cavity arranged along a first direction; the containing cavity is used for containing a single-hole test tube, a magnetic attraction unit used for generating a magnetic field is arranged in the containing cavity, and the magnetic attraction unit slides in the first direction; the hoop mechanism is arranged on the supporting body and used for fixing the height of the single-hole test tube in the first direction; the ultrasonic mechanism comprises an ultrasonic body, the ultrasonic body is arranged in the second direction, the ultrasonic body is arranged on the supporting body in a penetrating mode, and the ultrasonic body is used for making contact with the single-hole test tube and vibrating and heating reaction liquid in the single-hole test tube; according to the single-mode extraction module, the integration degree is high, the single-hole test tube can realize mixing, splitting decomposition and adsorption in the single-mode extraction module, the flow of transferring the single-hole test tube among a plurality of assemblies is canceled, the extraction time is shortened, and the extraction efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the field of nucleic acid extraction devices, in particular to a single-mode extraction module and a nucleic acid extraction device with a single-mode extraction module. Background Art

[0002] The magnetic bead method is a commonly used method in nucleic acid extraction experiments. In the magnetic bead method, a sample successively undergoes steps such as uniform mixing of the reaction solution, sample lysis, adsorption of nucleic acid to magnetic beads, etc., and then through magnetic bead washing and nucleic acid elution, finally purified nucleic acid is obtained. Correspondingly, the implementation of the magnetic bead method often relies on a nucleic acid extractor. Currently, nucleic acid extractors mainly include an oscillation component, a heating component, and a magnetic attraction component, with a low degree of integration. The multi-well test tube needs to rotate between the aforementioned components and operate in each component, and most of the time is wasted in the rotation process. On the one hand, the extraction efficiency is low; on the other hand, during the rotation process, it is also prone to contamination problems.

[0003] In addition, existing nucleic acid extractors need to be used in conjunction with multi-well test tubes, such as using 96-well deep plates to achieve multi-sample extraction of nucleic acids. When using a multi-well test tube for extraction, it is necessary to collect a sufficient number of samples in advance, which results in a large amount of time being spent on collecting a sufficient number of samples before nucleic acid extraction, and also indirectly leads to low extraction efficiency. Summary of the Utility Model

[0004] The utility model provides a single-mode extraction module and a nucleic acid extraction device, and its purpose is to improve the integration of the single-mode extraction module, cancel the rotation process of the single-hole test tube between components, and improve the efficiency of nucleic acid extraction.

[0005] To achieve the above purpose, an embodiment of the utility model provides a single-mode extraction module, including:

[0006] A magnetic attraction mechanism, including a support body, in which a receiving cavity is provided along a first direction; the receiving cavity is used to place a single-hole test tube, and a magnetic attraction unit for generating a magnetic field is further provided in the receiving cavity, and the magnetic attraction unit slides along the first direction;

[0007] A clamping mechanism, arranged on the support body, and the clamping mechanism is used to fix the height of the single-hole test tube in the first direction;

[0008] An ultrasonic mechanism, including an ultrasonic body, the ultrasonic body is arranged along a second direction, the ultrasonic body penetrates through the support body, and the ultrasonic body is used to contact the single-hole test tube and vibrate and heat the reaction solution in the single-hole test tube;

[0009] The first direction intersects with the second direction.

[0010] Preferably, the ultrasonic mechanism further includes an ultrasonic driving unit that drives the ultrasonic body to move in the second direction.

[0011] Preferably, the single-mode extraction module further includes a substrate, and the support body and the ultrasonic driving unit are arranged on the substrate;

[0012] The ultrasonic driving unit includes a slide rail and a linear actuator. The linear actuator and the slide rail are fixed on the substrate. The slide rail is arranged in the second direction, and an ultrasonic slide seat for fixing the ultrasonic body is further arranged on the slide rail. The output end of the linear actuator is connected to the ultrasonic slide seat to drive the ultrasonic slide seat to slide on the slide rail.

[0013] Preferably, a magnetic attraction driving unit is further arranged in the accommodation cavity, and the magnetic attraction driving unit drives the magnetic attraction unit to move in the first direction.

[0014] Preferably, the accommodation cavity includes a first section and a second section connected to the first section in the first direction. The first section is used for inserting a single-hole test tube, and the second section is used for accommodating the magnetic attraction driving unit and the magnetic attraction unit. The magnetic attraction unit is closer to the first section than the magnetic attraction driving unit;

[0015] A magnetic attraction slide seat is further arranged in the second section. The magnetic attraction slide seat is slidably arranged in the second section in the first direction, and the magnetic attraction unit is fixed on the magnetic attraction slide seat;

[0016] A shoulder is formed at the connection between the first section and the second section. The shoulder is located on the sliding path of the magnetic attraction slide seat, and the shoulder limits the maximum stroke of the magnetic attraction slide seat sliding towards the first section.

[0017] Preferably, the magnetic attraction unit is a permanent magnet, and the magnetic attraction driving unit is an electromagnet or a linear actuator.

[0018] Preferably, the hoop mechanism includes two symmetrically arranged clamping units. The clamping unit includes a fixed arc plate and a moving arc plate with the same bending direction. An elastic member for driving the fixed arc plate and the moving arc plate to separate is arranged between the fixed arc plate and the moving arc plate. The fixed arc plate is fixed at the upper end of the support body, and the two moving arc plates are used for clamping the single-hole test tube.

[0019] Preferably, a groove is arranged on the upper surface of the magnetic attraction unit, and the shape of the groove is the same as the bottom contour of the single-hole test tube.

[0020] Preferably, the first direction is perpendicular to the second direction.

[0021] The present application also provides a nucleic acid extraction device, which adopts the aforementioned single-mode extraction module, and the single-mode extraction module includes one or more.

[0022] The above - mentioned solution of the utility model has the following beneficial effects:

[0023] First, the integration level of this application is high. The single - well test tube can achieve mixing, lysis, and adsorption in the single - mode extraction module, eliminating the process of transporting the single - well test tube between multiple components, shortening the time required for extraction, and improving the extraction efficiency.

[0024] Second, the ultrasonic body contacts the single - well test tube to complete the oscillation and heating of the reaction solution. At the same time, by changing the distance between the magnetic attraction unit and the single - well test tube, the magnetic force of the magnetic attraction unit on the magnetic beads is adjusted. When it is necessary to attract the magnetic beads to one side of the single - well test tube, the distance between the magnetic attraction unit and the single - well test tube is reduced to ensure that the magnetic beads are adsorbed under the single - well test tube, realizing the separation of the magnetic beads from other substances in the reaction solution.

[0025] Third, this application is applicable to single - well test tubes and can achieve on - arrival and on - inspection without waiting.

[0026] Fourth, this application can be combined with multiple modules. The extraction processes between the modules are independent and do not interfere with each other.

[0027] Other features and advantages of the utility model will be described in detail in the subsequent specific implementation part. Description of the Drawings

[0028] Figure 1 is a schematic diagram of the utility model;

[0029] Figure 2 is Figure 1 a schematic diagram of the hidden housing;

[0030] Figure 3 is a cross - sectional view of the magnetic attraction mechanism;

[0031] Figure 4 is a schematic diagram of the hoop mechanism in the first direction.

[0032]

Description of the Reference Numerals

[0033] 100 - magnetic attraction mechanism, 110 - support body, 111a - first section, 111b - second section, 112 - penetration hole, 120 - magnetic attraction unit, 140 - magnetic attraction sliding seat,

[0034] 200 - hoop mechanism, 210 - fixed arc plate, 220 - movable arc plate, 230 - elastic member, 240 - limiting block

[0035] 300 - ultrasonic mechanism, 310 - ultrasonic body, 320 - ultrasonic driving unit, 321 - slide rail, 322 - linear actuator, 323 - ultrasonic sliding seat,

[0036] 400 - substrate,

[0037] 500 - Outer shell,

[0038] M - Single - hole test tube. Detailed implementation manner

[0039] To make the technical problems, technical solutions and advantages to be solved by the present utility model clearer, the following will be described in detail with reference to the accompanying drawings and specific embodiments.

[0040] As Figures 1-4 shown, an embodiment of the present utility model provides a single - mode extraction module, including a magnetic attraction mechanism 100, a hoop mechanism 200 and an ultrasonic mechanism 300. The magnetic attraction mechanism 100 includes a support body 110, and a receiving cavity is provided on the support body 110. The receiving cavity is arranged on the support body 110 along a first direction, and the receiving cavity is used for placing the single - hole test tube M. A magnetic attraction unit 120 is further arranged in the receiving cavity. The magnetic attraction unit 120 is used for generating a magnetic field to adsorb magnetic beads, and the magnetic attraction unit 120 can slide in the first direction. The aforementioned hoop mechanism 200 is arranged on the support body 110, and the hoop mechanism 200 is used for fixing the height of the single - hole test tube M in the first direction. The aforementioned ultrasonic mechanism 300 includes an ultrasonic body 310. The ultrasonic body 310 is arranged along a second direction, and the ultrasonic body 310 passes through the support body 110. The ultrasonic body 310 is in contact with the single - hole test tube M in the receiving cavity and can vibrate and heat the reaction solution in the single - hole test tube M.

[0041] The aforementioned first direction and the second direction intersect.

[0042] In this application, the single - hole test tube M is fixed in the receiving cavity by the hoop mechanism 200, and the heating and vibration of the reaction solution are realized through the contact between the ultrasonic body 310 and the single - hole test tube M. At the same time, the distance between the magnetic attraction unit 120 and the fixed single - hole test tube M can be changed by moving the magnetic attraction unit 120, so that the magnetic beads in the single - hole test tube M are affected by the magnetic force of the magnetic force unit and change.

[0043] In this application, the ultrasonic body 310 is used to realize vibration and heating, which promotes the mixing of the reaction solution and the sample and the separation of nucleic acid and protein, providing a basis for subsequent magnetic attraction treatment. During the magnetic attraction treatment, the magnetic attraction unit 120 moves along the first direction towards the single - hole test tube M, and the magnetic force on the magnetic beads in the single - hole test tube M increases, so that a number of magnetic beads are adsorbed on the bottom of the single - hole test tube M, achieving the effect of downward adsorption.

[0044] In this application, the integration level of the module is high. The ultrasonic mechanism 300, the magnetic attraction mechanism 100, and the hoop mechanism 200 are integrated in the same single-mode extraction module, and the steps of mixing, cracking, and adsorption can be realized in this module. During the extraction process, the single-hole test tube M is fixed in the accommodation cavity throughout. The ultrasonic mechanism 300 and the magnetic pole mechanism act on the reaction liquid in the single-hole test tube M in sequence. The single-hole test tube M does not need to rotate between different components, shortening the time required for nucleic acid extraction and improving the extraction efficiency. Since the single-hole test tube M does not need to rotate, the probability of contamination of the reaction liquid due to rotation is also reduced.

[0045] At the same time, by means of the ultrasonic body, the purposes of oscillation and heating are achieved, and the functions of the two components are integrated into one. The magnetic beads are adsorbed downward by the magnetic attraction unit 120 arranged below the single-hole test tube M. The arrangement of the ultrasonic body 310 and the magnetic attraction unit 120 makes full use of the space, streamlines the structure of the single-mode extraction module, reduces the volume of the single-mode extraction module, and realizes the miniaturization of the single-mode extraction module.

[0046] In addition, the single-mode extraction module is applicable to the single-hole test tube M, can perform on-site inspection as soon as the sample arrives, and does not require collecting a sufficient number of samples.

[0047] The aforementioned ultrasonic mechanism 300 further includes an ultrasonic driving unit 320, and the ultrasonic driving unit 320 drives the ultrasonic body 310 to move along the second direction. In this application, under the action of the ultrasonic driving unit 320, the ultrasonic body 310 can contact or separate from the single-hole test tube M in the second direction. When the single-hole test tube M is placed in the accommodation cavity, it can avoid the collision between the single-hole test tube M and the ultrasonic body 310 and damage the single-hole test tube M.

[0048] The single-mode extraction module further includes a substrate 400, and the support body 110 and the ultrasonic driving unit 320 are arranged on the substrate 400. The ultrasonic driving unit 320 includes a slide rail 321 and a linear actuator 322. Both the linear actuator 322 and the slide rail 321 are fixed on the substrate 400. The slide rail 321 extends along the second direction, and an ultrasonic slide seat 323 is further arranged on the slide rail 321. The slide rail 321 guides and positions the ultrasonic slide seat 323. The ultrasonic body 310 is fixed to the ultrasonic slide seat 323. The output end of the linear actuator 322 is connected to the ultrasonic slide seat 323. The linear actuator 322 realizes the purpose of changing the distance between the ultrasonic body 310 and the single-hole test tube M by driving the ultrasonic slide seat 323 to slide on the slide rail 321.

[0049] The aforementioned linear actuator 322 is a device that can convert the input energy into linear motion. The linear actuator 322 can be one of an electric linear actuator, a hydraulic linear actuator, or a pneumatic linear actuator.

[0050] When the linear pusher 322 is an electric linear pusher, the electric linear pusher includes a motor and a lead screw. The output end of the motor is in transmission connection with the lead screw, and the lead screw is screwed to the ultrasonic slide 323. By utilizing the screwing connection between the lead screw and the ultrasonic slide 323 and the limiting effect of the slide rail 321, the rotational motion output by the motor is converted into the linear motion of the ultrasonic slide 323, achieving the purpose of changing the distance between the ultrasonic body 310 and the single-hole test tube M.

[0051] Preferably, a reduction gear assembly can also be provided between the output end of the motor and the lead screw. The output end of the motor is in transmission connection with the input shaft of the reduction gear assembly, and the lead screw is in transmission connection with the output shaft of the reduction gear assembly. By providing the reduction gear assembly, the movement of the ultrasonic body 310 can be made more stable, avoiding collision between the ultrasonic body 310 and the single-hole test tube M and damaging the single-hole test tube M.

[0052] When the linear pusher 322 is a hydraulic linear pusher or a pneumatic linear pusher, the hydraulic linear pusher or the pneumatic linear pusher is fixed on the base, and the output end of the hydraulic linear pusher or the pneumatic linear pusher is connected to the ultrasonic slide 323. The linear motion of the output end is achieved by controlling the pressure of hydraulic oil or compressed air.

[0053] A magnetic attraction pushing unit is further provided in the aforementioned accommodation cavity. The magnetic attraction pushing unit pushes the magnetic attraction unit 120 to move in the first direction, thereby changing the magnitude of the magnetic force received by the magnetic beads. When the magnetic attraction unit 120 approaches the single-hole test tube M, the magnetic beads receive a larger magnetic force to complete the downward adsorption. When the magnetic attraction unit 120 is away from the single-hole test tube M, at this time, not affected by the magnetic force, the state of the magnetic beads is not affected by the magnetic attraction unit 120.

[0054] The aforementioned accommodation cavity includes a first section 111a and a second section 111b. The first section 111a and the second section 111b are connected end to end in sequence in the first direction. The first section 111a is used for inserting the single-hole test tube M, and the second section 111b is used for accommodating the magnetic attraction pushing unit and the magnetic attraction unit 120. The magnetic attraction unit 120 is closer to the first section 111a than the magnetic attraction pushing unit. The hoop mechanism 200 is provided on the end face of the first section 111a away from the second section 111b. Preferably, a probing hole 112 is provided on the support body 110. The probing hole 112 is arranged along the second direction and is communicated with the first section 111a. The probing hole 112 is used for the ultrasonic body 310 to extend into the first section 111a to contact the single-hole test tube M.

[0055] A magnetic attraction slide 140 is further provided in the second end. The magnetic attraction slide 140 is slidably arranged in the second section 111b along the first direction. The aforementioned magnetic attraction unit 120 is fixed on the magnetic attraction slide 140, and the magnetic attraction unit 120 realizes the movement along the first direction through the sliding of the magnetic attraction slide 140.

[0056] A shoulder is also formed at the connection between the first section 111a and the second section 111b. The shoulder is located on the sliding path of the magnetic attraction sliding seat 140 and limits the maximum stroke of the magnetic attraction sliding seat 140 sliding towards the first section 111a.

[0057] For better description, the plane perpendicular to the first direction is called the first plane. In this application, the support body 110 is rectangular, and the cross-sections of the first section 111a and the second section 111b along the first plane are square. The side length of the cross-section of the first section 111a is smaller than the side length of the cross-section of the second section 111b. The first section 111a and the second section 111b form a structure with a smaller upper part and a larger lower part. Therefore, a shoulder is formed at the connection between the first section 111a and the second section 111b. Correspondingly, the cross-section of the magnetic attraction sliding seat 140 along the first plane is also square, and the side length of the magnetic attraction sliding seat 140 is equal to the side length of the second section 111b, so that the magnetic attraction sliding seat 140 can slide within the second section 111b and is restricted by the inner wall of the support body 110 forming the second section 111b to prevent the magnetic attraction sliding seat 140 from rotating around the first direction.

[0058] It can be understood that the cross-sections of the first section 111a and the second section 111b along the first plane can also be circular. The diameter of the cross-section of the first section 111a is smaller than the diameter of the cross-section of the second section. The first section 111a and the second section 111b form a structure with a smaller upper part and a larger lower part. Therefore, a shoulder can also be formed at the connection between the first section 111a and the second section 111b. Correspondingly, the cross-section of the magnetic attraction sliding seat 140 along the first plane is also circular, and the diameter of the magnetic attraction sliding seat 140 is equal to the diameter of the second section 111b, so that the magnetic attraction sliding seat 140 can slide within the second section 111b.

[0059] The aforementioned shoulder is located on the sliding path of the magnetic attraction sliding seat 140. When the magnetic attraction sliding seat 140 moves to the shoulder, it reaches the maximum stroke of the magnetic attraction sliding seat 140. The magnetic attraction sliding seat 140 is restricted by the shoulder and cannot continue to slide, which can effectively prevent the magnetic attraction unit 120 from exceeding the maximum stroke and damaging the single-hole test tube M.

[0060] In this application, the magnetic attraction unit 120 is a permanent magnet, and the permanent magnet can provide a stable magnetic field. The magnetic attraction driving unit can be an electromagnet or a linear pusher 322.

[0061] If the magnetic attraction unit 120 is an electromagnet, the electromagnet is fixedly arranged below the permanent magnet. The electromagnet can change the magnetic poles by changing the direction of the input current. When it is necessary for the permanent magnet to move away from the single-hole test tube M, the magnetic pole above the electromagnet is opposite to the magnetic pole below the permanent magnet. At this time, the electromagnet and the permanent magnet attract each other, pulling the permanent magnet in the direction away from the single-hole test tube M. When it is necessary for the permanent magnet to approach the single-hole test tube M, the magnetic pole above the electromagnet is the same as the magnetic pole below the permanent magnet. At this time, the electromagnet and the permanent magnet repel each other, pushing the permanent magnet in the direction of the single-body test tube. During the movement of the permanent magnet in the direction of the single-body test tube, it stops moving due to the limitation of the shoulder.

[0062] If the magnetic attraction unit 120 is a linear pusher 322, the linear pusher 322 can be one of an electric linear pusher, a hydraulic linear pusher or a pneumatic linear pusher.

[0063] When the linear pusher 322 is one of a hydraulic linear pusher or a pneumatic linear pusher, and the cross-sections of the first section 111a and the second section 111b are square or circular, the output end of the hydraulic linear pusher or the pneumatic linear pusher is connected to the magnetic attraction sliding seat 140.

[0064] When the linear pusher 322 is an electric linear pusher, the electric linear pusher also includes a motor and a lead screw. The output end of the motor is connected to the lead screw, and the lead screw is screwed to the magnetic attraction sliding seat 140. If the cross-sections of the first section 111a and the second section 111b are circular, a guide rail is arranged on the inner wall of the support body 110 forming the second section 111b. The guide rail is arranged along the first direction, and the magnetic attraction sliding seat 140 is slidably arranged on the guide rail. The guide rail guides the sliding direction of the magnetic attraction sliding seat 140 and restricts the magnetic attraction sliding seat 140 from rotating around the first direction.

[0065] If the cross-sections of the first section 111a and the second section 111b are square, the magnetic attraction sliding seat 140 will not rotate around the first direction under the restriction of the inner wall of the support body 110 forming the second section 111b. Therefore, the guide rail can be not arranged.

[0066] The aforementioned hoop mechanism 200 includes two symmetrically arranged clamping units. Each clamping unit includes a fixed arc plate 210 and a movable arc plate 220. An elastic member 230 for driving the separation of the fixed arc plate 210 and the movable arc plate 220 is arranged between the fixed arc plate 210 and the movable arc plate 220. The bending directions of the fixed arc plate 210 and the movable arc plate 220 in each clamping unit are the same, and the bending directions of the fixed arc plates 210 in the two clamping units are opposite. The fixed arc plate 210 of each clamping unit is fixed on the support body 110, and the two movable arc plates 220 form an approximately circular structure for clamping the single-hole test tube M.

[0067] Further, a guide post is provided on the surface of the movable arc plate 220 facing the fixed arc plate 210. One end of the guide post is fixed on the movable arc plate 220, and the other end passes through the fixed arc plate 210. The elastic member 230 is sleeved on the guide post, and both ends of the elastic member 230 are respectively abutted against the fixed arc plate 210 and the movable arc plate 220, and the elastic member 230 is in a compressed state. A limit block 240 is also provided on the end of the guide post passing through the fixed arc plate 210, and the limit block 240 limits the separation of the fixed arc plate 210 from the guide post.

[0068] Preferably, a groove is provided on the upper surface of the magnetic attraction unit 120, and the shape of the groove is the same as the bottom contour of the single-hole test tube M. When the magnetic attraction unit 120 approaches the single-hole test tube M, the minimum distance between the two is that the groove fits with the bottom of the single-hole test tube M.

[0069] Preferably, the foregoing first direction and second direction are perpendicular to each other. When the ultrasonic body 310 moves along the second direction, it can achieve surface contact with the single-hole test tube M, so that the heating efficiency and transmission efficiency reach the maximum.

[0070] Preferably, the single-mode extraction module further includes a housing 500, and the housing 500 wraps the magnetic attraction mechanism 100 and the ultrasonic mechanism 300 therein.

[0071] The present application also provides a nucleic acid extraction device, including one or more of the foregoing single-mode extraction modules.

[0072] When the nucleic acid extraction device includes a single-mode extraction module, the nucleic acid extraction device may further include other functional modules, such as washing, dissolving and other modules.

[0073] When the nucleic acid extraction includes a plurality of single-mode extraction modules, the plurality of single-mode extraction modules can perform extraction independently without interfering with each other.

[0074] It should be understood that the nucleic acid extraction device may further include a plurality of single-mode extraction modules and other functional modules.

[0075] The above are the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle described in the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A single-mode extraction module, characterized in that: include: A magnetic attraction mechanism (100) comprises a support body (110), wherein the support body (110) is provided with a receiving cavity arranged along a first direction; the receiving cavity is used to place a single-hole test tube (M), and a magnetic attraction unit (120) is also arranged in the receiving cavity, and the magnetic attraction unit (120) slides along the first direction; A clamp mechanism (200) is arranged on the supporting body (110), and the clamp mechanism (200) is used to fix the height of the single-hole test tube (M) in a first direction; The ultrasonic mechanism (300) comprises an ultrasonic body (310), the ultrasonic body (310) being arranged along the second direction, the ultrasonic body (310) being arranged through the supporting body (110), the ultrasonic body (310) being used to contact the single-hole test tube (M) and vibrate and heat the reaction liquid in the single-hole test tube (M); The first direction intersects the second direction.

2. The single-mode extraction module according to claim 1, characterized in that: The ultrasonic mechanism (300) further includes an ultrasonic pushing unit (320), and the ultrasonic pushing unit (320) pushes the ultrasonic body (310) to move along the second direction.

3. The single-mode extraction module according to claim 2, characterized in that: The single-mode extraction module further comprises a substrate (400), and the support body (110) and the ultrasonic pushing unit (320) are arranged on the substrate (400); The ultrasonic pushing unit (320) comprises a slide rail (321) and a linear pusher (322); the linear pusher (322) and the slide rail (321) are fixed on the base plate (400); the slide rail (321) is arranged along the second direction; an ultrasonic slide seat (323) for fixing the ultrasonic body (310) is also arranged on the slide rail (321); the output end of the linear pusher (322) is connected to the ultrasonic slide seat (323) to push the ultrasonic slide seat (323) to slide on the slide rail (321).

4. The single-mode extraction module according to claim 1, characterized in that: A magnetic attraction pushing unit is also provided in the accommodating cavity, and the magnetic attraction pushing unit pushes the magnetic attraction unit (120) to move in a first direction.

5. The single-mode extraction module according to claim 4, characterized in that: The accommodating cavity comprises a first section (111a) and a second section (111b) connected to the first section (111a) in a first direction, the first section (111a) being used for inserting a single-hole test tube (M), the second section (111b) being used for accommodating the magnetic attraction pushing unit and the magnetic attraction unit (120), the magnetic attraction unit (120) being closer to the first section (111a) than the magnetic attraction pushing unit; A magnetic attraction slide (140) is also provided in the second section (111b), and the magnetic attraction slide (140) is slidably provided in the second section (111b) along the first direction, and the magnetic attraction unit (120) is fixed on the magnetic attraction slide (140); The first section (111a) and the second section (111b) form a shoulder at the connection, and the shoulder is located on the sliding path of the magnetic attraction slide (140). The shoulder limits the maximum sliding stroke of the magnetic attraction slide (140) toward the first section (111a).

6. The single-mode extraction module according to claim 5, characterized in that: The magnetic attraction unit (120) is a permanent magnet, and the magnetic attraction pushing unit is an electromagnet or a linear pusher (322).

7. The single-mode extraction module according to claim 1, characterized in that: The clamp mechanism (200) comprises two symmetrically arranged clamping units, the clamping units comprising a fixed arc plate (210) and a moving arc plate (220) having the same bending direction, an elastic member (230) for driving the fixed arc plate (210) and the moving arc plate (220) to separate is arranged between the fixed arc plate (210) and the moving arc plate (220), the fixed arc plate (210) is fixed to the upper end of the supporting body (110), and the two moving arc plates (220) are used to clamp a single-hole test tube (M).

8. The single-mode extraction module according to claim 1, characterized in that: The upper surface of the magnetic attraction unit (120) is provided with a groove, and the shape of the groove is the same as the bottom profile of the single-hole test tube (M).

9. The single-mode extraction module according to claim 1, characterized in that: The first direction and the second direction are perpendicular to each other.

10. A nucleic acid extraction device, using the single-mode extraction module according to any one of claims 1 to 9, characterized in that: The single-mode extraction module includes one or more.

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