Special two-groove cell counting plate

Abstract

The utility model discloses a plate special for two-groove cell counting, and belongs to the technical field of biological cell counting. A special two-groove cell counting plate comprises a top pressing plate and further comprises a double-faced adhesive tape layer fixed below the top pressing plate, and a glass bottom plate fixedly connected below the double-faced adhesive tape layer. A plurality of semicircular air holes and rectangular air holes are formed in the top pressing plate, and a plurality of rectangular groove positions are formed in the double-sided adhesive layer; the semicircular air hole and the rectangular air hole are respectively positioned above the inner sides of the two wide edges of the rectangular slot; the semicircular air holes and the rectangular air holes are respectively communicated with the rectangular slot positions; the number of the rectangular air holes and the number of the semicircular air holes are both two, and the number of the rectangular groove positions formed in the double-faced adhesive tape layer is two. According to the cell counting plate disclosed by the utility model, the assembly process of a traditional non-fixed counting plate is omitted, so that the assembly time is saved, and meanwhile, the error of a test result caused by the abrasion of the sample plate in the fitting assembly process of the cell counting plate can be reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of biological cell counting, in particular to a special plate for two-slot cell counting. Background Art

[0002] With the development of the biological cell field in society, experiments such as cell culture, cell proliferation and migration, cell transfection, and cell immunofluorescence have received more and more extensive attention and applications. Cell counting plays an indispensable role in this.

[0003] In most existing microscopes or electronic instruments for cell counting, non-fixed cell sample slides or carriers have problems such as cumbersome operations, different amounts of cell samples taken, and cell damage during use. The loosening of components and the complexity of operations directly affect the efficiency and accuracy of cell counting. Based on this, the present utility model is proposed. Summary of the Utility Model

[0004] The technical problem to be solved by the present utility model is to overcome the deficiencies of the prior art and provide a special plate for two-slot cell counting that can overcome the above problems or at least partially solve the above problems.

[0005] In order to achieve the above object, the present utility model adopts the following technical solutions:

[0006] A special plate for two-slot cell counting includes a top pressing plate, and further includes: a double-sided adhesive layer is fixed below the top pressing plate, and a glass bottom plate is fixedly connected below the double-sided adhesive layer; a plurality of semicircular air holes and rectangular air holes are respectively opened on the top pressing plate, and a plurality of rectangular slots are opened on the double-sided adhesive layer; the semicircular air holes and rectangular air holes are respectively located above the inner sides of the two wide sides of the rectangular slot; the semicircular air holes and rectangular air holes are respectively communicated with the rectangular slot.

[0007] Preferably, the number of rectangular air holes and semicircular air holes opened on the top pressing plate is both two.

[0008] Further, the number of rectangular slots opened on the double-sided adhesive layer is two, and each rectangular slot corresponds to a rectangular air hole and a semicircular air hole.

[0009] Preferably, the two rectangular slots are arranged longitudinally.

[0010] Preferably, the glass bottom plate is made of a transparent material, and the shapes of the top pressing plate, the double-sided adhesive layer, and the glass bottom plate are the same.

[0011] Preferably, chamfers are provided around the top pressing plate, the double-sided adhesive layer, and the glass bottom plate.

[0012] Preferably, the long side of the rectangular slot is [X] to [Y] times the width side, and the long sides of the top pressing plate, the double-sided adhesive layer and the glass bottom plate are [X] to [Y] times the width side.

[0013] Preferably, the width side of the rectangular air hole is equal to the diameter of the semi-circular air hole.

[0014] Compared with the prior art, the present utility model provides a special two-slot cell counting plate, which has the following beneficial effects:

[0015] 1. This special two-slot cell counting plate is integrated by three layers of materials, without the need for assembly operations, which is simple and time-saving. At the same time, it also reduces the wear of the sample plate caused during the fitting and assembly of the cell counting plate, resulting in errors in the test results.

[0016] In the device, the parts not involved are the same as or can be implemented by the prior art. The present utility model omits the assembly process of the traditional non-fixed counting plate, which not only saves assembly time but also reduces the wear of the sample plate caused during the fitting and assembly of the cell counting plate, resulting in errors in the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a schematic structural diagram of a special two-slot cell counting plate proposed by the present utility model;

[0018] Figure 2 is a top view of the top pressing plate in a special two-slot cell counting plate proposed by the present utility model;

[0019] Figure 3 is a top view of the double-sided adhesive layer in the bottom of a special two-slot cell counting plate proposed by the present utility model;

[0020] Figure 4 is a top view of the glass bottom plate in the housing structure of a special two-slot cell counting plate proposed by the present utility model.

[0021] In the figure: 1. Top pressing plate; 2. Double-sided adhesive layer; 3. Glass bottom plate; 11. Rectangular air hole; 12. Semi-circular air hole; 21. Rectangular slot. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments.

[0023] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model.

[0024] Embodiment 1:

[0025] Referring to Figures 1-4 , a special two-slot cell counting plate includes a top pressing plate 1, and further includes: a double-sided adhesive layer 2 is fixed below the top pressing plate 1, and a glass bottom plate 3 is fixedly connected below the double-sided adhesive layer 2; a plurality of semi-circular air holes 12 and rectangular air holes 11 are respectively opened on the top pressing plate 1, and a plurality of rectangular slots 21 are opened on the double-sided adhesive layer 2; the semi-circular air holes 12 and the rectangular air holes 11 are respectively located above the inner sides of the two wide sides of the rectangular slot 21; the semi-circular air holes 12 and the rectangular air holes 11 are respectively communicated with the rectangular slot 21, the number of rectangular air holes 11 and semi-circular air holes 12 opened on the top pressing plate 1 is two, the number of rectangular slots 21 opened on the double-sided adhesive layer 2 is two, each rectangular slot 21 corresponds to a rectangular air hole 11 and a semi-circular air hole 12, the two rectangular slots 21 are arranged longitudinally, the glass bottom plate 3 is made of a transparent material, the top pressing plate 1, the double-sided adhesive layer 2 and the glass bottom plate 3 have the same shape, chamfers are provided on the peripheries of the top pressing plate 1, the double-sided adhesive layer 2 and the glass bottom plate 3, the long side of the rectangular slot 21 is 5 to 7 times the width side, the long side of the top pressing plate 1, the double-sided adhesive layer 2 and the glass bottom plate 3 is 3 to 5 times the width side, and the width side of the rectangular air hole 11 is equal to the diameter of the semi-circular air hole 12.

[0026] In the present utility model, the whole device consists of three layers: the upper layer is the top pressing plate 1, the middle layer is the double-sided adhesive layer 2, and the lower layer is the glass bottom plate 3. The glass bottom plate 3 is double-sided transparent. The top pressing plate 1 and the bottom glass bottom plate 3 are adhered together through the double-sided adhesive layer 2. The rectangular air hole 11 on the top pressing plate 1 provides a non-sealed exhaust hole for sample injection, and the semi-circular air hole 12 is the injection hole for the test sample. While the double-sided adhesive layer 2 adheres the upper and lower plates, the reserved rectangular slots 21 also provide a certain slot thickness for placing and observing cell samples;

[0027] The sizes of the semi-circular air hole 12 and the rectangular air hole 11 on the top pressing plate 1 result in better hydrophilicity during sample injection, making it more convenient to inject the sample. The design of the two different air holes on both sides not only facilitates the injection of the sample, but also reduces the sample loss caused by the shaking of the cell plate while retaining the air holes, thereby affecting the test results.

[0028] The top pressing plate 1, the double-sided adhesive layer 2, and the glass bottom plate 3 are fixedly adhered to each other and are a set of fixed plates in both the used and unused states. When the use is completed, just pinch the blank part on the plate surface and slowly pull it out.

[0029] Preferably, the long sides of the top pressing plate 1, the double-sided adhesive layer 2, and the glass bottom plate 3 are all 96.00 mm, the wide sides are all 25.50 mm, the chamfer radius is 1.00 mm, the long side of the rectangular slot 21 is 30.00 mm, the wide side is 5.00 mm, the distance between the rectangular slot 21 and the adjacent slot is 3.00 mm, and the distance between two rectangular air holes 11 is 33.00 mm. The middle distance between a single pair of rectangular air holes 11 and semi-circular air holes 12 is 26.50 mm. The adjacent distance of the semi-circular air holes 12 is 33.00 mm.

[0030] When in use, the user needs to confirm in advance whether the detection instrument is correctly connected and can be used normally. Then, the user can take out this device to perform sample detection. First, the sample to be tested needs to be injected into the rectangular slot 21 through the semi-circular air hole 12. During the injection process, the speed should be kept as slow as possible to avoid squeezing the test sample out of the rectangular slot 21. After the injection is completed, pinch the blank end of this device, keep the direction of the top pressing plate 1 upward, and gently place it into the cell counter. Push the cell counter to the bottom to start the test. After the use is completed, it can be gently pulled out by pinching the blank end of this device. Then, pour out the test sample inside the rectangular slot 21 through the semi-circular air hole 12 and the rectangular air hole 11, and disinfect and clean it with medical alcohol. After the cleaning is completed, this device can be sealed for use in the next experiment.

[0031] The above is only the preferred specific implementation manner of the present utility model, but the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present utility model, according to the technical solution of the present utility model and its inventive concept, makes equivalent substitutions or changes, and all should be covered within the protection scope of the present utility model.

Claims

1. A special plate for two-slot cell counting, comprising a top pressing plate (1), characterized in that, It further includes: A double-sided adhesive layer (2) is fixed below the top pressing plate (1), and a glass bottom plate (3) is fixedly connected below the double-sided adhesive layer (2); A plurality of semi-circular air holes (12) and rectangular air holes (11) are respectively formed on the top pressing plate (1), and a plurality of rectangular slots (21) are formed on the double-sided adhesive layer (2); The semi-circular air holes (12) and the rectangular air holes (11) are respectively located above the inner sides of the two wide sides of the rectangular slot (21); The semi-circular air holes (12) and the rectangular air holes (11) are respectively communicated with the rectangular slot (21).

2. The special plate for two-slot cell counting according to claim 1, characterized in that, The number of the rectangular air holes (11) and the semi-circular air holes (12) formed on the top pressing plate (1) is two each.

3. The special plate for two-slot cell counting according to claim 2, characterized in that The number of the rectangular slots (21) formed on the double-sided adhesive layer (2) is two, and each rectangular slot (21) corresponds to a rectangular air hole (11) and a semi-circular air hole (12).

4. A special plate for two-slot cell counting according to claim 1, characterized in that, The two rectangular slots (21) are arranged longitudinally.

5. A dedicated plate for two-slot cell counting according to claim 1, characterized in that, The glass bottom plate (3) is made of a transparent material, and the top pressing plate (1), the double-sided adhesive layer (2) and the glass bottom plate (3) have the same shape.

6. The dedicated two-groove cell counting plate according to claim 1, characterized in that, Chamfers are provided on the peripheries of the top pressing plate (1), the double-sided adhesive layer (2) and the glass bottom plate (3).

7. A dedicated plate for two-slot cell counting according to claim 1, characterized in that The long side of the rectangular slot (21) is 5 to 7 times the width side, and the long side of the top pressing plate (1), the double-sided adhesive layer (2) and the glass bottom plate (3) is 3 to 5 times the width side.

8. A special plate for two-slot cell counting according to claim 7, characterized in that, The width side of the rectangular air hole (11) is equal to the diameter of the semi-circular air hole (12).

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