Preparation device of TMAO negative sample

Abstract

The utility model relates to the technical field of TMAO negative samples, and discloses a TMAO negative sample preparation device which comprises a workbench, stand columns are fixedly connected to the four corners of the bottom wall of the workbench, a carrier plate is fixedly connected between every two adjacent stand columns, a rotating motor is fixedly connected to the right side of the top wall of each carrier plate, and the rotating motor is fixedly connected to the right side of the bottom wall of the workbench. The output end of the rotating motor is fixedly connected with a driving gear, the outer wall of the driving gear is in meshed connection with a toothed belt, the inner wall of the toothed belt is in meshed connection with a plurality of driven gears at equal intervals, and the top ends of the driven gears are fixedly connected with cleaning columns. According to the cleaning device, the liquid after cleaning flows back to the water tank from the filtering backflow pipe, TMAO mixed in the cleaning liquid can be filtered out, it is ensured that the cleaning liquid flowing back to the water tank is relatively clean, recycling is facilitated, the utilization rate of the cleaning liquid is increased, and meanwhile waste of resources is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of TMAO negative samples, in particular to a preparation device for TMAO negative samples. Background Technique

[0002] TMAO, namely trimethylamine N-oxide, and a TMAO negative sample refers to a sample in which the content of trimethylamine N-oxide is lower than the detection limit after specific treatment or detection. In medical and biological research, by comparing TMAO positive samples and negative samples, the role and influence mechanism of trimethylamine N-oxide in certain physiological processes and the occurrence and development of diseases can be understood more deeply.

[0003] After retrieval, the Chinese patent publication number is: CN209727566U, a preparation device for TMAO negative samples, including a filtering device. The filtering device is provided with a cavity inside, a liquid inlet at the top, and a liquid outlet at the bottom. The liquid inlet and the liquid outlet are both communicated with the cavity, and the cavity is filled with a filler. The filler is a solid matrix loaded with Reinecke salt. The solid matrix is a magnetic sphere, a silica sphere, a magnetic bead, a mesoporous molecular sieve, a zeolite molecular sieve or other solid matrix. Using the principle of molecular sieve, after the biological sample fully reacts with Reinecke salt, the precipitate formed by TMAO and the Reinecke salt reagent stays on the surface of the solid matrix, so as to achieve the function of only removing TMAO in biological samples such as plasma while keeping other components in the plasma unchanged. The device has a simple structure and is easy to operate, and has a good removal effect, overcoming the defect that it is difficult to prepare TMAO negative samples in the prior art. However, after the sample preparation, the device cannot clean the sample carrier in time. After a long time, the residual sample substances will gradually accumulate on the carrier, and at the same time, new samples will be contaminated due to cross-contamination, thereby affecting the accuracy of the experimental results. Content of the Utility Model

[0004] In order to make up for the above deficiencies, the utility model provides a preparation device for TMAO negative samples, aiming to improve the problem that after the sample preparation, the sample carrier cannot be cleaned in time, which will cause the residual sample substances to gradually accumulate on the carrier after a long time, and at the same time, new samples will be contaminated due to cross-contamination, thereby affecting the accuracy of the experimental results.

[0005] To achieve the above object, the present utility model adopts the following technical solutions: A device for preparing a TMAO-negative sample, comprising a workbench, wherein columns are fixedly connected to the four corners of the bottom wall of the workbench, a carrier plate is fixedly connected between adjacent ones of the multiple columns, a rotating motor is fixedly connected to the right side of the top wall of the carrier plate, a driving gear is fixedly connected to the output end of the rotating motor, a toothed belt is meshed with the outer wall of the driving gear, multiple driven gears are equidistantly meshed with the inner wall of the toothed belt, cleaning columns are fixedly connected to the tops of the multiple driven gears, the middle upper parts of the outer walls of the multiple cleaning columns penetrate through the inner bottom wall of the workbench, a water tank is fixedly connected to the middle of the top wall of the carrier plate, a water pump is communicated with the left side of the water tank, a shunt pipe is communicated with the top of the water pump, multiple spray pipes are equidistantly communicated with the top of the shunt pipe, the multiple spray pipes all penetrate through the interiors of the multiple cleaning columns, a filtering and reflux pipe is communicated with the right end of the bottom wall of the workbench, the left end of the filtering and reflux pipe is communicated with the right side of the water tank, multiple ultrasonic generators are equidistantly fixedly connected to the front and rear sides of the inner wall of the workbench, and a preparation mechanism is arranged on the left side of the top wall of the workbench.

[0006] Through the above technical solutions: The rotating motor drives the driving gear and multiple driven gears to rotate, providing the rotational force for cleaning the sample carrier. The liquid after cleaning flows back to the water tank through the filtering and reflux pipe, which can filter out the TMAO mixed in the cleaning liquid, ensuring that the cleaning liquid flowing back to the water tank is relatively clean for re-circulation use, improving the utilization rate of the cleaning liquid and reducing the waste of resources at the same time.

[0007] As a further description of the above technical solutions:

[0008] The preparation mechanism includes a bracket, a sample tank is fixedly connected to the top of the bracket, a filter pipe is communicated with the bottom end of the sample tank, a filter element is threadedly connected to the inner wall of the filter pipe, a knob head is fixedly connected to the top end of the filter element, a sponge base is fixedly connected to the left side of the inner bottom wall of the workbench, a U-shaped sliding groove is fixedly connected to the middle upper part of the inner wall of the workbench, a storage cup is slidably connected to the inner wall of the U-shaped sliding groove, and the bottom end of the storage cup is attached to the inner top wall of the sponge base.

[0009] Through the above technical solutions: Filtration is carried out through the filter pipe communicated with the bottom end of the sample tank. Rotating the knob head at the top of the filter tank can conveniently install and replace the filter element. The filtered sample will drip into the storage cup below, realizing the filtration and storage of the TMAO-negative sample and providing a good basis for experiments and analysis.

[0010] As a further description of the above technical solutions:

[0011] A console is fixedly connected to the middle of the front side of the workbench, and multiple control buttons are fixedly installed on the top of the console.

[0012] Through the above technical solution: The console is used to centrally control the various operating parameters and operating modes of the entire device, and adjust the rotation motor, ultrasonic generator, and timer respectively through multiple control buttons on its top.

[0013] As a further description of the above technical solution:

[0014] Anti-slip pads are fixedly connected to the bottoms of multiple said columns, and multiple said anti-slip pads are square structures.

[0015] Through the above technical solution: The anti-slip pads at the bottoms of the columns can increase the friction between the device and the placement plane, preventing the device from sliding or shifting due to the vibration of the equipment or external forces during the working process.

[0016] As a further description of the above technical solution:

[0017] A timer is fixedly connected to the front side of the workbench, and the timer is electrically connected to the rotation motor and the console.

[0018] Through the above technical solution: The timer is electrically connected to the rotation motor and the console, and is used to precisely control the cleaning time. Appropriate cleaning durations can be set according to different samples and cleaning requirements.

[0019] As a further description of the above technical solution:

[0020] An arc-shaped lock is rotatably connected to the left side of the top wall of the U-shaped chute, and a fixed bolt penetrates through the top wall of the arc-shaped lock.

[0021] Through the above technical solution: The arc-shaped lock and the fixed bolt on the left side of the top wall of the U-shaped chute are used to fix the storage cup, preventing the storage cup from accidentally sliding or falling during the cleaning or sample transfer process, and ensuring the safe storage and transfer of the sample.

[0022] As a further description of the above technical solution:

[0023] A sealing cover is rotatably connected to the right top end of the sample groove, and a handle is fixedly connected to the right side of the sealing cover.

[0024] Through the above technical solution: The sealing cover at the right top end of the sample groove can be conveniently rotated through the handle, and can be closed after the sample is placed, reducing the entry of external impurities into the sample groove and ensuring the purity of the sample.

[0025] As a further description of the above technical solution:

[0026] Sealing rings are fixedly connected to the middle parts of the outer walls of multiple said cleaning columns, and the bottoms of multiple said sealing rings are attached to the inner bottom wall of the workbench.

[0027] Through the above technical solution: The sealing ring in the middle of the outer wall of the cleaning column can prevent the cleaning liquid from leaking at the connection between the cleaning column and the workbench, ensuring the effective utilization of the cleaning liquid during the cleaning process.

[0028] The utility model has the following beneficial effects:

[0029] 1. In the utility model, the driving gear and multiple driven gears are driven to rotate by the rotating motor, providing the driving force for the cleaning of the sample carrier. The liquid after cleaning flows back to the water tank through the filter return pipe, which can filter out the TMAO mixed in the cleaning liquid, ensuring that the cleaning liquid flowing back to the water tank is relatively clean for reuse, improving the utilization rate of the cleaning liquid and reducing the waste of resources at the same time.

[0030] 2. In the utility model, filtration is carried out through the filter pipe connected to the bottom end of the sample slot. The knob at the top of the rotating filter tank can be conveniently used to install and replace the filter element. The filtered sample will drip into the storage cup below, realizing the filtration and storage of TMAO-negative samples, providing a good basis for experiments and analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 is a perspective view of a device for preparing TMAO-negative samples proposed by the utility model;

[0032] Figure 2 is a structural schematic diagram of the rotating motor of a device for preparing TMAO-negative samples proposed by the utility model;

[0033] Figure 3 is a structural schematic diagram of the driven gear of a device for preparing TMAO-negative samples proposed by the utility model;

[0034] Figure 4 is a disassembled view of the structure of the filter element of a device for preparing TMAO-negative samples proposed by the utility model;

[0035] Figure 5 is Figure 4 an enlarged view of the structure at A in

[0036] LEGEND DESCRIPTION:

[0037] 1. Workbench; 2. Preparation mechanism; 201. Bracket; 202. Sample tank; 203. Filter tube; 204. Filter element; 205. Knob head; 206. Sponge base; 207. U-shaped chute; 208. Storage cup; 3. Column; 4. Carrier plate; 5. Rotating motor; 6. Driving gear; 7. Tooth belt; 8. Driven gear; 9. Cleaning column; 10. Water tank; 11. Water pump; 12. Shunt pipe; 13. Spray pipe; 14. Filter return pipe; 15. Ultrasonic generator; 16. Console; 17. Control button; 18. Anti-slip pad; 19. Timer; 20. Arc-shaped lock; 21. Fixed pin; 22. Sealing cover; 23. Handle; 24. Sealing ring. Detailed implementation manners

[0038] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0039] Referring to Figure 1 , Figure 2 and Figure 3 , an embodiment provided by the present invention: A preparation device for TMAO-negative samples includes a workbench 1. Columns 3 are fixedly connected to the four corners of the bottom wall of the workbench 1. Carrier plates 4 are fixedly connected between adjacent ones of the multiple columns 3. A rotating motor 5 is fixedly connected to the right side of the top wall of the carrier plate 4. The output end of the rotating motor 5 is fixedly connected to a driving gear 6. The outer wall of the driving gear 6 is meshed with a tooth belt 7. Multiple driven gears 8 are equidistantly meshed with the inner wall of the tooth belt 7. Cleaning columns 9 are fixedly connected to the tops of the multiple driven gears 8. The upper middle parts of the outer walls of the multiple cleaning columns 9 penetrate through the inner bottom wall of the workbench 1. A water tank 10 is fixedly connected to the middle of the top wall of the carrier plate 4. A water pump 11 is connected to the left side of the water tank 10 in communication. A shunt pipe 12 is connected to the top of the water pump 11 in communication. Multiple spray pipes 13 are equidistantly connected to the top of the shunt pipe 12 in communication. The multiple spray pipes 13 all penetrate through the interiors of the multiple cleaning columns 9. A filter return pipe 14 is connected to the right end of the bottom wall of the workbench 1 in communication. The left end of the filter return pipe 14 is connected to the right side of the water tank 10 in communication. Multiple ultrasonic generators 15 are equidistantly fixedly connected to the front and rear sides of the inner wall of the workbench 1. A preparation mechanism 2 is arranged on the left side of the top wall of the workbench 1;

[0040] Specifically, after starting the rotation motor 5, the output end thereof drives the driving gear 6 to rotate. The driving gear 6 drives a plurality of driven gears 8 to rotate simultaneously through the toothed belt 7 meshing therewith. Since the top end of the driven gear 8 is fixedly connected to the cleaning column 9, during the rotation of the driven gear 8, the cleaning column 9 also rotates accordingly. The rotation of the cleaning column 9 provides the rotational force for cleaning the sample carrier, making the cleaning more comprehensive and thorough. At the same time, the cleaning liquid in the water tank 10 is pumped out under the action of the water pump 11, and the cleaning liquid is pushed to the shunt pipe 12. The shunt pipe 12 then evenly distributes the cleaning liquid into each equally spaced and connected spray pipe 13. Since the spray pipe 13 penetrates through the inside of the cleaning column 9, the cleaning liquid can be accurately sprayed into the sample carrier inside the rotating cleaning column 9. The sample carrier can be fully rinsed through the mutual cooperation of the rotating cleaning column 9 and the sprayed cleaning liquid. During the cleaning process, the ultrasonic waves generated by a plurality of ultrasonic generators 15 evenly distributed on the front and rear sides of the inner wall of the workbench 1 can propagate in the cleaning liquid to form tiny cavitation bubbles. These cavitation bubbles will generate a strong impact force when they burst, further removing the residual TMAO on the surface of the sample carrier and improving the quality and efficiency of cleaning. The cleaned liquid flows back to the water tank 10 through the filter return pipe 14 at the right end of the bottom wall of the workbench 1. The filter return pipe 14 can filter out the mixed TMAO in the cleaning liquid, ensuring that the cleaning liquid flowing back to the water tank 10 is relatively clean for reuse, improving the utilization rate of the cleaning liquid and reducing the waste of resources at the same time.

[0041] Refer to Figure 1 and Figure 4 , the preparation mechanism 2 includes a bracket 201. The top end of the bracket 201 is fixedly connected with a sample tank 202. The bottom end of the sample tank 202 is communicated with a filter pipe 203. The inner wall of the filter pipe 203 is threadedly connected with a filter element 204. The top end of the filter element 204 is fixedly connected with a knob head 205. The left side of the inner bottom wall of the workbench 1 is fixedly connected with a sponge base 206. The middle upper part of the inner wall of the workbench 1 is fixedly connected with a U-shaped sliding groove 207. The inner wall of the U-shaped sliding groove 207 is slidably connected with a storage cup 208. The bottom end of the storage cup 208 is attached to the inner top wall of the sponge base 206;

[0042] Specifically, when it is necessary to filter a sample, place the sample in the sample slot 202 fixedly connected to the top of the bracket 201. The sample will be filtered through the filter tube 203 connected to the bottom end of the sample slot 202. By rotating the knob head 205 at the top, the filter element 204 can be conveniently installed and replaced. The filtered sample will drip into the storage cup 208 below. The sponge base 206 fixedly connected to the left side of the inner bottom wall of the workbench 1 can provide a certain buffer and stable support for the storage cup 208, reduce the impact on the storage cup 208, and protect the integrity of the sample. At the same time, the U-shaped sliding groove 207 fixedly connected to the upper middle part of the inner wall of the workbench 1 provides guidance and limitation for the placement and movement of the storage cup 208. The storage cup 208 can slide smoothly in the inner wall of the U-shaped sliding groove 207, facilitating the operator to accurately place it in the appropriate position and ensuring that the sample can accurately fall into the storage cup 208, realizing the filtration and storage of TMAO-negative samples and providing a good basis for experiments and analyses.

[0043] Refer to Figure 1 and Figure 2 , a control console 16 is fixedly connected to the middle part of the front side of the workbench 1, and a plurality of control buttons 17 are fixedly installed on the top of the control console 16; anti-slip pads 18 are fixedly connected to the bottom ends of the plurality of columns 3, and the plurality of anti-slip pads 18 are of a square structure; a timer 19 is fixedly connected to the front side of the workbench 1, and the timer 19 is electrically connected to the rotation motor 5 and the control console 16;

[0044] Specifically, the control console 16 is used to centrally control the operation parameters and operating modes of the entire device, and adjust the rotation motor 5, the ultrasonic generator 15, and the timer 19 respectively through the plurality of control buttons 17 on its top. The anti-slip pads 18 at the bottom ends of the columns 3 can increase the friction between the device and the placement plane, preventing the device from sliding or shifting due to the vibration of the equipment or external forces during the working process. The timer 19 is electrically connected to the rotation motor 5 and the control console 16 and is used to accurately control the cleaning time. The appropriate cleaning duration can be set according to different samples and cleaning requirements.

[0045] Refer to Figure 1 , Figure 4 and Figure 5 , a curved latch 20 is rotatably connected to the left side of the top wall of the U-shaped sliding groove 207, and a fixing pin 21 penetrates through the top wall of the curved latch 20; a sealing cover 22 is rotatably connected to the right top end of the sample slot 202, and a handle 23 is fixedly connected to the right side of the sealing cover 22; sealing rings 24 are fixedly connected to the middle parts of the outer walls of the plurality of cleaning columns 9, and the bottoms of the plurality of sealing rings 24 are attached to the inner bottom wall of the workbench 1;

[0046] Specifically, the arc-shaped latch 20 and the fixed bolt 21 on the left side of the top wall of the U-shaped chute 207 are used to fix the storage cup 208, preventing the storage cup 208 from accidentally sliding or falling during the cleaning or sample transfer process, ensuring the safe storage and transfer of the sample. The sealing cover 22 at the top right of the sample chute 202 can be conveniently rotated through the handle 23, which can be closed after the sample is placed, reducing the entry of external impurities into the sample chute 202 and ensuring the purity of the sample. The sealing ring 24 in the middle of the outer wall of the cleaning column 9 can prevent the cleaning liquid from leaking at the connection between the cleaning column 9 and the workbench 1, ensuring the effective utilization of the cleaning liquid during the cleaning process.

[0047] Working principle: After starting the rotation motor 5, its output drives the drive gear 6 to rotate. The drive gear 6 drives multiple driven gears 8 to rotate simultaneously through the toothed belt 7 engaged with it. Since the top of the driven gear 8 is fixedly connected to the cleaning column 9, during the rotation of the driven gear 8, the cleaning column 9 also rotates accordingly. The rotation of the cleaning column 9 provides the rotational force for cleaning the sample carrier, making the cleaning more comprehensive and thorough. At the same time, the cleaning liquid in the water tank 10 is pumped out under the action of the water pump 11, and the cleaning liquid is pushed to the shunt pipe 12. The shunt pipe 12 then evenly distributes the cleaning liquid into each equally spaced and connected spray pipe 13. Since the spray pipe 13 penetrates the inside of the cleaning column 9, the cleaning liquid can be accurately sprayed into the sample carrier inside the rotating cleaning column 9. The rotating cleaning column 9 and the sprayed cleaning liquid can cooperate with each other to fully wash the sample carrier. During the cleaning process, the ultrasonic waves generated by multiple ultrasonic generators 15 evenly distributed on the front and back sides of the inner wall of the workbench 1 can propagate in the cleaning liquid, forming tiny cavitation bubbles. These cavitation bubbles will generate a strong impact force when they burst, further removing the residual TMAO on the surface of the sample carrier and improving the quality and efficiency of the cleaning. The cleaning liquid after cleaning flows back to the water tank 10 through the filter return pipe 14 at the right end of the bottom wall of the workbench 1. The filter return pipe 14 can filter out the TMAO mixed in the cleaning liquid, ensuring that the cleaning liquid flowing back to the water tank 10 is relatively clean for reuse.

[0048] Moreover, when it is necessary to filter the sample, place the sample in the sample slot 202 fixedly connected to the top of the bracket 201. The sample will be filtered through the filter tube 203 connected to the bottom end of the sample slot 202. By rotating the knob head 205 at the top, the filter element 204 can be conveniently installed and replaced. The filtered sample will drip into the storage cup 208 below. The sponge base 206 fixedly connected to the left side of the inner bottom wall of the workbench 1 can provide certain buffering and stable support for the storage cup 208, reduce the impact on the storage cup 208, and protect the integrity of the sample. At the same time, the U-shaped chute 207 fixedly connected to the upper middle part of the inner wall of the workbench 1 provides guidance and limitation for the placement and movement of the storage cup 208. The storage cup 208 can slide smoothly in the inner wall of the U-shaped chute 207, facilitating the operator to accurately place it in the appropriate position to ensure that the sample can fall into the storage cup 208 accurately without error.

[0049] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A preparation device for TMAO-negative samples, comprising a workbench (1), characterized in that: At the four corners of the bottom wall of the workbench (1), columns (3) are fixedly connected. Between adjacent ones of the multiple columns (3), a carrier plate (4) is fixedly connected. On the right side of the top wall of the carrier plate (4), a rotating motor (5) is fixedly connected. The output end of the rotating motor (5) is fixedly connected with a driving gear (6). The outer wall of the driving gear (6) is meshed with a toothed belt (7). At equal intervals on the inner wall of the toothed belt (7), a plurality of driven gears (8) are meshed. At the top of each of the multiple driven gears (8), a cleaning column (9) is fixedly connected. The upper middle parts of the outer walls of the multiple cleaning columns (9) penetrate through the inner bottom wall of the workbench (1). In the middle of the top wall of the carrier plate (4), a water tank (10) is fixedly connected. On the left side of the water tank (10), a water pump (11) is communicated. The top of the water pump (11) is communicated with a shunt pipe (12). At equal intervals at the top of the shunt pipe (12), a plurality of spray pipes (13) are communicated. Each of the multiple spray pipes (13) penetrates through the inside of each of the multiple cleaning columns (9). At the right end of the bottom wall of the workbench (1), a filtering and reflux pipe (14) is communicated. The left end of the filtering and reflux pipe (14) is communicated on the right side of the water tank (10). On the front and rear sides of the inner wall of the workbench (1), a plurality of ultrasonic generators (15) are fixedly connected at equal intervals. On the left side of the top wall of the workbench (1), a preparation mechanism (2) is arranged.

2. The preparation device for a TMAO-negative sample according to claim 1, wherein: The preparation mechanism (2) includes a bracket (201). At the top of the bracket (201), a sample tank (202) is fixedly connected. At the bottom end of the sample tank (202), a filter pipe (203) is communicated. Inside the filter pipe (203), a filter element (204) is threadedly connected. At the top of the filter element (204), a knob head (205) is fixedly connected. On the left side of the inner bottom wall of the workbench (1), a sponge base (206) is fixedly connected. In the upper middle part of the inner wall of the workbench (1), a U-shaped chute (207) is fixedly connected. Inside the U-shaped chute (207), a storage cup (208) is slidably connected. The bottom end of the storage cup (208) is attached to the inner top wall of the sponge base (206).

3. The preparation device for a TMAO-negative sample according to claim 1, characterized in that: In the middle of the front side of the workbench (1), a control console (16) is fixedly connected. On the top of the control console (16), a plurality of control buttons (17) are fixedly installed.

4. The preparation device for a TMAO-negative sample according to claim 1, characterized in that: At the bottom ends of the multiple columns (3), anti-slip pads (18) are fixedly connected. The multiple anti-slip pads (18) are square structures.

5. The preparation device for a TMAO-negative sample according to claim 1, characterized in that: On the front side of the workbench (1), a timer (19) is fixedly connected. The timer (19) is electrically connected to the rotating motor (5) and the control console (16).

6. The preparation device for a TMAO-negative sample according to claim 2, wherein: On the left side of the top wall of the U-shaped chute (207), an arc-shaped lock (20) is rotatably connected. Through the top wall of the arc-shaped lock (20), a fixing bolt (21) is penetrated.

7. The preparation device for a TMAO-negative sample according to claim 2, characterized in that: At the right top of the sample tank (202), a sealing cover (22) is rotatably connected. On the right side of the sealing cover (22), a handle (23) is fixedly connected.

8. The preparation device for a TMAO-negative sample according to claim 1, wherein: In the middle parts of the outer walls of the multiple cleaning columns (9), sealing rings (24) are fixedly connected. The bottoms of the multiple sealing rings (24) are attached to the inner bottom wall of the workbench (1).

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