Pipette for cell test
By designing a pipette for cell tests with pressure control tubes and regulation components, the problem of inaccurate liquid inhalation in the pipette in cell tests was solved, precise control and efficient operation were achieved, and the accuracy of experimental data was improved.
Patent Information
- Application Number
- CN202422194664.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-09
- Publication Date
- 2025-07-18
- Estimated Expiration
- 2034-09-09
AI Technical Summary
Existing pipettes are difficult to achieve precise control of trace liquids in cell experiments, resulting in excessive or too little inhalation, affecting the accuracy of experimental data, and time-consuming and labor-intensive operation.
A pipette for cell tests is designed, including a pressure control tube, a piston rod, a compression spring, a check valve and a control component. By adjusting the position of the upper limit plate, the lower floating plate is pushed to connect with the upper limit plate with the liquid to achieve precise control of the liquid volume and avoid excessive or too little inhalation.
It achieves an improvement in the accuracy of liquid inhalation, simple operation, time and effort, reduces the impact on cell experimental data, and improves work efficiency.
Smart Images
Figure CN223113101U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of pipettes, and particularly relates to a pipette for cell experiments. Background Art
[0002] The working principle of a pipette is mainly based on atmospheric pressure and the siphon phenomenon of liquids. By manually or with the aid of an instrument (such as a pipettor) to generate negative pressure, the liquid is sucked into the pipette, and then through controlling devices such as pressure or pistons, the liquid is accurately transferred or dispensed into the target container. In actual use, when the liquid is sucked into the pipette, the position of the liquid is usually measured by using the scale line to determine the amount of liquid sucked. However, sometimes there are still problems of excessive or insufficient suction. Micro-control operations are difficult, time-consuming and laborious, with low efficiency. And if the suction amount is inaccurate, it will directly lead to the inconsistency between the added reagent amount and the expectation, affecting the accuracy of cell experiment data. Content of the Utility Model
[0003] The utility model provides a pipette for cell experiments, which has the characteristics of facilitating the control of the liquid suction amount and improving the accuracy of experimental data.
[0004] The utility model provides the following technical solution: It includes a pipette and a bulb. A pressure control tube is arranged at the top end of the pipette. The pressure control tube is communicated with the internal space of the pipette. A piston rod is slidably connected to the inner wall of the pressure control tube. A compression spring is fixedly connected to the bottom end of the piston rod. An exhaust hole is opened on one side of the pressure control tube. A one-way valve is installed in the exhaust hole. A ventilation hole is opened on the piston rod. The compression spring is connected to the external space through the ventilation hole. A regulation component is arranged in the bulb. The regulation component includes an upper limit plate and a lower floating plate. Both the upper limit plate and the lower floating plate are slidably connected to the inner wall of the bulb. The lower floating plate contacts the bottom end of the upper limit plate.
[0005] Wherein, a connecting plug is fixedly connected to the bottom end of the pressure control tube. The connecting plug is inserted into the top end of the pipette. The pressure control tube is communicated with the internal space of the pipette through the connecting plug.
[0006] Wherein, a through hole is opened on the upper limit plate. A plurality of hollow holes are opened on the lower floating plate. The positions of the through hole and the plurality of hollow holes do not correspond.
[0007] Wherein, a sealing convex plate is fixedly connected to the top end of the lower floating plate. The sealing convex plate is snap-fitted to the inner wall of the through hole.
[0008] Wherein, a screw rod is rotatably connected to the top end of the upper limit plate. The screw rod is threadedly connected to the inner wall of the bulb.
[0009] Among them, a docking component is arranged between the pipette and the pressure control tube. The docking component includes an upper connecting plate and a lower connecting plate. The upper connecting plate is installed on the side wall of the pressure control tube, and the lower connecting plate is installed on the side wall of the pipette. The top of the upper connecting plate is in contact with the top of the lower connecting plate.
[0010] Among them, two protrusions are fixedly connected to the bottom end of the upper connecting plate, two grooves are formed in the top end of the lower connecting plate, the protrusions are inserted into the inner walls of the grooves, and the screw rod is not in contact with both the upper connecting plate and the lower connecting plate.
[0011] The beneficial effects of the present utility model are as follows: By adjusting the position height of the upper limiting plate, the amount of liquid that can be extracted is adjusted. The liquid pushes the lower floating plate to dock with the upper limiting plate, so that the amount of liquid extracted inside the pipette and the spherical part cannot continue to increase, thereby avoiding the problems of excessive or insufficient inhalation amount. The micro control operation is relatively simple, time-saving and labor-saving, the working efficiency is improved, and the accuracy of the inhalation amount is improved, which can reduce the influence on the accuracy of cell experiment data. By pressing the piston rod, the extraction can be achieved, and the liquid can be discharged by releasing the hand. The operation is simple and convenient.
[0012] Parts not involved in this device are the same as the prior art or can be implemented by using the prior art. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 is the three-dimensional exploded structure schematic diagram of the present utility model;
[0014] Figure 2 is the front sectional structure schematic diagram of the present utility model;
[0015] Figure 3 is the three-dimensional enlarged structure schematic diagram of the regulation component in the present utility model;
[0016] Figure 4 is Figure 1 the enlarged schematic diagram of part A in
[0017] In the figure: 1. Pipette; 11. Spherical part; 2. Pressure control tube; 21. Piston rod; 211. Vent hole; 22. Compression spring; 23. Connecting plug; 24. Exhaust hole; 3. Regulation component; 31. Upper limiting plate; 311. Through hole; 32. Lower floating plate; 321. Hollow hole; 322. Sealing convex plate; 33. Screw rod; 4. Docking component; 41. Upper connecting plate; 411. Protrusion; 42. Lower connecting plate; 421. Groove. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0018] Please refer to Figures 1-4, the present utility model provides the following technical solutions: It includes a pipette 1 and a bulb 11. A pressure control tube 2 is provided at the top of the pipette 1. The pressure control tube 2 is communicated with the internal space of the pipette 1. A piston rod 21 is slidably connected to the inner wall of the pressure control tube 2. The bottom end of the piston rod 21 is fixedly connected to a compression spring 22. An exhaust hole 24 is provided on one side of the pressure control tube 2. A check valve is installed in the exhaust hole 24. A ventilation hole 211 is provided on the piston rod 21. The compression spring 22 is connected to the external space through the ventilation hole 211. A regulation assembly 3 is provided inside the bulb 11. The regulation assembly 3 includes an upper limit plate 31 and a lower floating plate 32. Both the upper limit plate 31 and the lower floating plate 32 are slidably connected to the inner wall of the bulb 11. The lower floating plate 32 contacts the bottom end of the upper limit plate 31.
[0019] In this implementation: The pipette 1 and the bulb 11 form a pipetting container body. The pipette 1 inhales and discharges the liquid for cell experiments through the bottom opening. The scale lines on the pipette 1 and the bulb 11 facilitate determining and comparing the height of the liquid level. The pressure control tube 2 is located at the top of the pipette 1. The pressure control tube 2 and the pipette 1 are designed to be detachable and separable, which is convenient for cleaning and maintenance. The pressure control tube 2 controls the internal air pressure of the pipette 1, enabling the pipette 1 to draw and transfer liquid. The piston rod 21 controls the internal air pressure of the pressure control tube 2. By pressing the piston rod 21, the finger blocks the ventilation hole 211. The piston rod 21 squeezes the air inside the pressure control tube 2. The pressure control tube 2 discharges the gas through the exhaust hole 24, enabling a negative pressure to be formed inside the pipette 1 and the pressure control tube 2. The compression spring 22 pushes the piston rod 21 to slide upward and reset. The negative pressure draws the liquid into the pipette 1 and the bulb 11. The check valve in the exhaust hole 24 blocks the air entering from the outside, thereby preventing the liquid inside the pipette 1 from being discharged. By pressing the piston rod 21 multiple times, the pipette 1 can stably draw the liquid until the required amount is reached, and then the liquid is transferred. When discharging the liquid inside the pipette 1 and the bulb 11, by releasing the blockage of the ventilation hole 211, the external air can enter the pressure control tube 2 and the pipette 1 through the ventilation hole 211, thereby enabling the liquid inside the pipette 1 to be naturally discharged. The operation is simple and convenient. Both the upper limit plate 31 and the lower floating plate 32 are inside the bulb 11. As the liquid drawn in increases, the lower floating plate 32 can be pushed upward by the liquid. The position of the upper limit plate 31 is designed to be adjustable. By controlling the position of the upper limit plate 31 inside the bulb 11, the maximum range that the lower floating plate 32 can slide upward is restricted. When the liquid pushes the lower floating plate 32 to the position of the upper limit plate 31, the upper limit plate 31 blocks the liquid through the lower floating plate 32, preventing the liquid suction volume inside the pipette 1 and the bulb 11 from continuing to increase, thus avoiding the problem of excessive or insufficient suction volume. The micro-control operation is relatively simple, time-saving and labor-saving, the work efficiency is improved, and the accuracy of the suction volume is increased, which can reduce the impact on the accuracy of cell experiment data.
[0020] A connecting plug 23 is fixedly connected to the bottom end of the pressure control tube 2. The connecting plug 23 is inserted into the top end of the pipette 1. The pressure control tube 2 is communicated with the internal space of the pipette 1 through the connecting plug 23. The pressure control tube 2 is connected to the pipette 1 through the connecting plug 23. After the connecting plug 23 is inserted into the pipette 1, the pipette 1 supports the pressure control tube 2 through the connecting plug 23. Moreover, the connection between the connecting plug 23 and the pipette 1 can increase the tightness between the pressure control tube 2 and the pipette 1 and improve the use stability.
[0021] Through holes 311 are formed in the upper limit plate 31, and a plurality of hollow holes 321 are formed in the lower floating plate 32. The positions of the through holes 311 do not correspond to those of the plurality of hollow holes 321. The through holes 311 and the plurality of hollow holes 321 enable the upper limit plate 31 and the lower floating plate 32 to have the condition of air ventilation when used separately, maintaining balance. And the non-alignment of the through holes 311 and the hollow holes 321 causes the through holes 311 not to form an air ventilation condition when the upper limit plate 31 and the lower floating plate 32 are in contact, thereby realizing water blocking, preventing the liquid from passing over the upper limit plate 31 and playing a role in quantitative measurement.
[0022] A sealing convex plate 322 is fixedly connected to the top end of the lower floating plate 32. The sealing convex plate 322 is snap-connected to the inner wall of the through hole 311. The sealing convex plate 322 corresponds to the through hole 311 in the up and down position. When the liquid level pushes the lower floating plate 32 to reach the position of the upper limit plate 31, the upper limit plate 31 blocks and limits the lower floating plate 32. The lower floating plate 32 drives the sealing convex plate 322 to insert into the through hole 311, so that the through hole 311 can be blocked and closed by the sealing convex plate 322, improving the stability of quantitative liquid extraction.
[0023] A screw rod 33 is rotatably connected to the top end of the upper limit plate 31. The screw rod 33 is threadedly connected to the inner wall of the spherical part 11. The spherical part 11 supports the upper limit plate 31 through the screw rod 33. The threaded connection between the screw rod 33 and the spherical part 11 enables the screw rod 33 to slide in the up and down direction by itself under the influence of the spherical part 11 when rotating. The screw rod 33 drives the upper limit plate 31 to adjust the up and down position, so that the upper limit plate 31 can adjust the liquid extraction amount.
[0024] A docking assembly 4 is arranged between the pipette 1 and the pressure control tube 2. The docking assembly 4 includes an upper connecting plate 41 and a lower connecting plate 42. The upper connecting plate 41 is installed on the side wall of the pressure control tube 2, and the lower connecting plate 42 is installed on the side wall of the pipette 1. The top ends of the upper connecting plate 41 and the lower connecting plate 42 are in contact. The pipette 1 supports the lower connecting plate 42, and the pressure control tube 2 supports the upper connecting plate 41. When the pressure control tube 2 and the pipette 1 are docked, the connecting plug 23 is inserted into the pipette 1. At the same time, the upper connecting plate 41 and the lower connecting plate 42 are in close contact. By holding the upper connecting plate 41 and the lower connecting plate 42, the connection between the pressure control tube 2 and the pipette 1 can be kept in a more stable state.
[0025] Two protrusions 411 are fixedly connected to the bottom end of the upper connecting plate 41, and two grooves 421 are formed at the top end of the lower connecting plate 42. The protrusions 411 are inserted into the inner wall of the grooves 421, and the screw rod 33 is not in contact with either the upper connecting plate 41 or the lower connecting plate 42. The upper connecting plate 41 supports the two protrusions 411. When the upper connecting plate 41 and the lower connecting plate 42 are butted, the upper connecting plate 41 drives the two protrusions 411 to insert into the grooves 421, so that the lower connecting plate 42 can limit the protrusions 411. The protrusions 411 drive the upper connecting plate 41 to remain fixed, preventing rotational sliding between the upper connecting plate 41 and the lower connecting plate 42, improving the stability when the upper connecting plate 41 and the lower connecting plate 42 are connected, and forming a dislocation between the screw rod 33 and the upper connecting plate 41 and the lower connecting plate 42, so that they will not interfere with each other.
[0026] The working principle and usage process of the present utility model: When the device is used, the pressure control tube 2 is held and butted with the pipette 1. The pressure control tube 2 drives the connecting plug 23 to insert into the top end of the pipette 1. At the same time, the upper connecting plate 41 drives the two protrusions 411 to insert into the grooves 421, so that the lower connecting plate 42 can limit the protrusions 411, preventing rotational sliding between the upper connecting plate 41 and the lower connecting plate 42, and improving the stability when the upper connecting plate 41 and the lower connecting plate 42 are connected. By pressing the piston rod 21, the finger blocks the air vent 211. The piston rod 21 squeezes the air inside the pressure control tube 2, and the pressure control tube 2 discharges the gas through the exhaust hole 24, so that a negative pressure can be formed inside the pipette 1 and the pressure control tube 2. The compression spring 22 pushes the piston rod 21 to slide upward and reset. The negative pressure sucks the liquid into the pipette 1 and the bulb 11. The one-way valve in the exhaust hole 24 blocks the air entering from the outside, so as to prevent the liquid inside the pipette 1 from being discharged. By pressing the piston rod 21 multiple times, the pipette 1 can stably suck the liquid. When the liquid pushes the lower floating plate 32 to the position of the upper limit plate 31, the upper limit plate 31 and the lower floating plate 32 block each other, and the amount of liquid sucked reaches the required amount.
Claims
1. A pipette for cell experiments, comprising a pipette (1) and a bulb (11), characterized in that: A pressure control tube (2) is provided at the top of the pipette (1). The pressure control tube (2) is in communication with the internal space of the pipette (1). A piston rod (21) is slidably connected to the inner wall of the pressure control tube (2). A compression spring (22) is fixedly connected to the bottom end of the piston rod (21). An exhaust hole (24) is formed on one side of the pressure control tube (2), and a one-way valve is installed in the exhaust hole (24). A ventilation hole (211) is formed on the piston rod (21). The compression spring (22) is connected to the external space through the ventilation hole (211). A regulation assembly (3) is arranged in the spherical part (11). The regulation assembly (3) includes an upper limit plate (31) and a lower floating plate (32). Both the upper limit plate (31) and the lower floating plate (32) are slidably connected to the inner wall of the spherical part (11), and the lower floating plate (32) contacts the bottom end of the upper limit plate (31).
2. The pipette for cell experiments according to claim 1, wherein: A connecting plug (23) is fixedly connected to the bottom end of the pressure control tube (2). The connecting plug (23) is inserted into the top end of the pipette (1). The pressure control tube (2) is in communication with the internal space of the pipette (1) through the connecting plug (23).
3. A pipette for cell experiments according to claim 1, characterized in that: A through hole (311) is formed on the upper limit plate (31), and a plurality of hollow holes (321) are formed on the lower floating plate (32). The position of the through hole (311) does not correspond to that of the plurality of hollow holes (321).
4. A pipette for cell experiments according to claim 3, characterized in that: A sealing convex plate (322) is fixedly connected to the top end of the lower floating plate (32). The sealing convex plate (322) is snap-connected to the inner wall of the through hole (311).
5. A pipette for cell experiments according to claim 1, wherein: A screw (33) is rotatably connected to the top end of the upper limit plate (31). The screw (33) is threadedly connected to the inner wall of the spherical part (11).
6. A pipette for cell experiments according to claim 5, characterized in that: A docking assembly (4) is arranged between the pipette (1) and the pressure control tube (2). The docking assembly (4) includes an upper connecting plate (41) and a lower connecting plate (42). The upper connecting plate (41) is installed on the side wall of the pressure control tube (2), and the lower connecting plate (42) is installed on the side wall of the pipette (1). The top ends of the upper connecting plate (41) and the lower connecting plate (42) are in contact with each other.
7. A pipette for cell experiments according to claim 6, characterized in that: Two protrusions (411) are fixedly connected to the bottom end of the upper connecting plate (41). Two grooves (421) are formed on the top end of the lower connecting plate (42). The protrusions (411) are inserted into the inner walls of the grooves (421). The screw (33) is not in contact with the upper connecting plate (41) and the lower connecting plate (42).