Electrochemical incubation detection module

Abstract

The utility model provides an electrochemical incubation detection module, which relates to the technical field of electrochemical detection and is characterized in that a reaction cavity is arranged in a micro-fluidic chip assembly, when the reaction temperature needs to be adjusted, the temperature is controlled through the matching of a heating assembly and a heat dissipation assembly, and in the whole temperature control and temperature change process, the reaction temperature can be adjusted. The real-time responsiveness is high, the temperature control precision is high, the stability is good, and the accuracy of electrochemical detection after amplification is improved; the temperature change control is carried out only in one temperature zone, so that the space occupation volume of the whole machine is reduced, the convenience is increased, the whole machine instrument is more exquisite, the liquid movement is not involved, namely, the risk of liquid in a moving pipeline is avoided, the loss of a reaction reagent is reduced, and the usability is improved; the technical problems that in the prior art, traditional PCR generally can only achieve the isothermal function, a plurality of temperature zones and liquid flow channels need to be designed for the variable temperature function, and cost is high are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of electrochemical detection, in particular to an electrochemical incubation detection module. Background Technique

[0002] PCR is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication outside the organism. The biggest feature of PCR is that it can greatly increase the amount of trace DNA.

[0003] Traditional PCR usually can only achieve isothermal function, while the variable temperature function requires the design of multiple temperature zones and liquid flow channels, resulting in a high cost. Content of the Utility Model

[0004] The purpose of the utility model is to provide an electrochemical incubation detection module, so as to alleviate the technical problem in the prior art that traditional PCR usually can only achieve isothermal function, while the variable temperature function requires the design of multiple temperature zones and liquid flow channels, resulting in a high cost.

[0005] The electrochemical incubation detection module provided by the utility model includes: a heat dissipation component, a heating component and a microfluidic chip component;

[0006] The microfluidic chip component has a reaction cavity;

[0007] The heating component covers the microfluidic chip component, and the heating component is used to heat the reaction cavity;

[0008] The heat dissipation component is arranged on the top of the heating component, and the heat dissipation component is configured to be able to dissipate and transfer the heat of the reaction cavity, so as to cooperate with the heating component to control the temperature of the reaction cavity.

[0009] In an optional embodiment,

[0010] The heat dissipation component includes a heat dissipation fan and a heat dissipation member;

[0011] The heat dissipation member is arranged on the top of the heating component;

[0012] The heat dissipation fan is arranged on the side of the heat dissipation member away from the heating component, and the heat dissipation fan is used to transfer the heat in the reaction cavity.

[0013] In an optional embodiment,

[0014] The heat dissipation member has a plurality of heat dissipation fins, and the plurality of heat dissipation fins are independently and spaced apart.

[0015] In an optional embodiment,

[0016] The microfluidic chip assembly includes a microfluidic chip fixing cover plate, an electrode sheet, and a microfluidic chip base;

[0017] The electrode sheet is disposed on the microfluidic chip base;

[0018] The microfluidic chip fixing cover plate covers the electrode sheet, and the microfluidic chip fixing cover plate is connected to the microfluidic chip base.

[0019] In an alternative embodiment,

[0020] The microfluidic chip fixing cover plate has a through hole, and a reaction chamber is formed by surrounding between the inner wall of the through hole and the electrode sheet.

[0021] In an alternative embodiment,

[0022] The microfluidic chip assembly further includes a sealing ring;

[0023] The sealing ring is disposed in the through hole, and the sealing ring is used to concentrate the reagent in the reaction chamber.

[0024] In an alternative embodiment,

[0025] The microfluidic chip fixing cover plate is provided with a liquid injection channel and an exhaust channel;

[0026] Both the liquid injection channel and the exhaust channel communicate with the through hole;

[0027] The liquid injection channel is used to inject liquid into the through hole, and the exhaust channel is used to discharge the gas in the through hole.

[0028] In an alternative embodiment,

[0029] The microfluidic chip assembly further includes an electrode signal receiving end and a microfluidic chip card holder;

[0030] The electrode signal receiving end is electrically connected to the electrode sheet;

[0031] The microfluidic chip card holder is disposed at the bottom of the microfluidic chip base, and the microfluidic chip card holder is used to clamp the microfluidic chip base.

[0032] In an alternative embodiment,

[0033] The heating assembly includes a connecting member and a heating sheet;

[0034] The connecting member is connected to the heat dissipation assembly, and the connecting member is used to fix the heating sheet so that the heating sheet is located above the microfluidic chip assembly.

[0035] In an alternative embodiment,

[0036] The connecting member includes a left fixing clip and a right fixing clip;

[0037] Both the left fixing clip and the right fixing clip are connected to the bottom of the heat dissipation component;

[0038] Both sides of the heating sheet are respectively lapped on the left fixing clip and the right fixing clip.

[0039] The electrochemical incubation detection module provided by the present utility model sets a reaction chamber in the microfluidic chip assembly. When it is necessary to adjust the reaction temperature, temperature control is carried out through the cooperation of the heating component and the heat dissipation component. During the whole temperature control and temperature change process, high real-time responsiveness, high temperature control precision, and good stability can be achieved, improving the accuracy of electrochemical detection after amplification; since only variable temperature control is performed in one temperature zone, the occupied volume of the whole machine is reduced in space, the convenience is increased, the whole machine instrument is more delicate, and since there is no movement of liquid, that is, there is no risk of liquid in the moving pipeline, the loss of reaction reagents is reduced, the availability is improved, and the technical problem existing in the prior art that traditional PCR can usually only achieve isothermal function, while variable temperature function requires the design of multiple temperature zones and liquid flow channels and has a high cost is alleviated. Description of the Drawings

[0040] In order to more clearly illustrate the specific embodiments of the present utility model or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the following drawings are some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained according to these drawings.

[0041] Figure 1 It is an exploded view of the overall structure of the electrochemical incubation detection module provided by the embodiment of the present utility model;

[0042] Figure 2 It is an exploded view of the structure of the microfluidic chip assembly in the electrochemical incubation detection module provided by the embodiment of the present utility model;

[0043] Figure 3 It is a schematic diagram of the installation of the sealing ring in the electrochemical incubation detection module provided by the embodiment of the present utility model;

[0044] Figure 4 It is a schematic diagram of the structure of the microfluidic chip assembly in the electrochemical incubation detection module provided by the embodiment of the present utility model.

[0045] Icons: 100 - heat dissipation component; 110 - heat dissipation fan; 120 - heat dissipation member; 200 - heating component; 210 - left fixing clip; 220 - right fixing clip; 230 - heating sheet; 300 - microfluidic chip component; 310 - microfluidic chip fixing cover plate; 311 - through hole; 312 - liquid injection channel; 313 - exhaust channel; 320 - electrode sheet; 330 - microfluidic chip base; 340 - sealing ring; 350 - electrode signal receiving end; 360 - microfluidic chip card seat. Detailed implementation manners

[0046] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are some but not all of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0047] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be construed as a limitation of the present utility model. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0048] In the description of the present utility model, it should be noted that unless otherwise clearly defined and limited, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0049] The following will describe in detail the specific implementation manners of the present utility model with reference to the accompanying drawings. It should be understood that the specific implementation manners described herein are only used to illustrate and explain the present utility model and are not used to limit the present utility model.

[0050] As Figure 1As shown in the figure, the electrochemical incubation detection module provided in this embodiment includes: a heat dissipation component 100, a heating component 200, and a microfluidic chip component 300; the microfluidic chip component 300 has a reaction chamber; the heating component 200 covers the microfluidic chip component 300, and the heating component 200 is used to heat the reaction chamber; the heat dissipation component 100 is arranged on the top of the heating component 200, and the heat dissipation component 100 is configured to be able to dissipate and transfer the heat of the reaction chamber to cooperate with the heating component 200 to control the temperature of the reaction chamber.

[0051] The electrochemical incubation detection module provided in this embodiment sets a reaction chamber in the microfluidic chip component 300. When the reaction temperature needs to be adjusted, temperature control is carried out through the cooperation of the heating component 200 and the heat dissipation component 100. During the whole temperature control and temperature change process, it can achieve high real-time responsiveness, high temperature control accuracy, and good stability, improving the accuracy of electrochemical detection after amplification; since only variable temperature control is carried out in one temperature zone, the occupied volume of the whole machine is reduced in space, increasing convenience, and the whole machine instrument is more delicate. And because there is no liquid movement involved, that is, there is no risk of liquid in the moving pipeline, the loss of reaction reagents is reduced, the availability is improved, and it alleviates the technical problem in the prior art that traditional PCR can usually only achieve isothermal function, while variable temperature function requires the design of multiple temperature zones and liquid flow channels, with a high cost.

[0052] Regarding the structure and shape of the heating component 200, specifically:

[0053] The heating component 200 includes a connecting member and a heating sheet 230; the connecting member is connected to the heat dissipation component 100, and the connecting member is used to fix the heating sheet 230 to locate the heating sheet 230 above the microfluidic chip component 300. The connecting member specifically includes a left fixing clip 210 and a right fixing clip 220. The left fixing clip 210 and the right fixing clip 220 have the same structure and are arranged on both sides of the heating sheet 230, one on the left and one on the right. Both the left fixing clip 210 and the right fixing clip 220 are in a convex-shaped structure, and the protrusions are connected to the bottom of the heat dissipation component 100. Both sides of the heating sheet 230 are respectively lapped on the left fixing clip 210 and the right fixing clip 220, so as to fix the heating sheet 230 above the microfluidic chip component 300, and the heating sheet 230 is electrified to heat the reaction chamber.

[0054] Regarding the structure and shape of the heat dissipation component 100, specifically:

[0055] The heat dissipation component 100 includes a heat dissipation fan 110 and a heat dissipation member 120; the heat dissipation member 120 is disposed on the top of the heating component 200. The heat dissipation member 120 is specifically configured as a fin heat sink, having a plurality of heat dissipation fins. The plurality of heat dissipation fins are independently and spaced apart. The heat dissipation fan 110 is disposed on the side of the heat dissipation member 120 away from the heating component 200. The heat dissipation fan 110 is used to transfer the heat in the reaction chamber, and can optionally cooperate with a temperature control algorithm for precise temperature control. For example, a temperature control circuit board is designed, and a temperature control algorithm of multi-module PID and PWM combined control PWM mode is used to control the operation of the heating sheet 230. The heated temperature is returned to the temperature control board through a temperature sensor for processing and correction. The temperature control board outputs different powers to control the heating sheet 230. At the same time, the heat dissipation fins use a multi-level design to greatly increase the specific surface area of heat dissipation. The heat is transferred by the fan to achieve the purpose of precise temperature regulation. Due to its single temperature zone design, the jump between multiple temperatures is sensitive and fast, achieving the effect of low-delay and high-precision temperature control.

[0056] Regarding the structure and shape of the microfluidic chip component 300, specifically:

[0057] As Figure 2 、 Figure 4 shown, the microfluidic chip component 300 includes a microfluidic chip fixing cover plate 310, an electrode sheet 320, and a microfluidic chip base 330; the electrode sheet 320 is disposed on the microfluidic chip base 330; the microfluidic chip fixing cover plate 310 is covered on the electrode sheet 320, and the microfluidic chip fixing cover plate 310 is connected to the microfluidic chip base 330. Specifically, connecting plates are formed by bending both sides of the microfluidic chip fixing cover plate 310. Connecting holes are opened on the connecting plates. A clamping protrusion is disposed on the side of the microfluidic chip base 330. The clamping protrusion extends into the connecting holes, and the microfluidic chip fixing cover plate 310 can be connected to the microfluidic chip base 330.

[0058] In addition, optionally, a groove is opened on the top surface of the microfluidic chip base 330, and the electrode sheet 320 is placed in the groove so that the top surface of the electrode sheet 320 is flush with the top surface of the microfluidic chip base 330.

[0059] The microfluidic chip fixing cover plate 310 has a through hole 311. The through hole 311 is opened at the middle position of the microfluidic chip fixing cover plate 310. A reaction chamber is formed by surrounding between the inner wall of the through hole 311 and the electrode sheet 320.

[0060] As Figure 3 shown, in order to prevent the reagent liquid in the reaction chamber from leaking, the microfluidic chip component 300 further includes a sealing ring 340; the sealing ring 340 is disposed in the through hole 311, and the sealing ring 340 is in close fit with the electrode sheet 320. The sealing ring 340 concentrates the reagent in the reaction chamber.

[0061] In an alternative embodiment, the fixed cover plate 310 of the microfluidic chip is provided with a liquid injection channel 312 and an exhaust channel 313; both the liquid injection channel 312 and the exhaust channel 313 communicate with the through hole 311; the liquid injection channel 312 is used to inject liquid into the through hole 311, and the exhaust channel 313 is used to discharge the gas in the through hole 311. The channel size of the liquid injection channel 312 is larger than that of the exhaust channel 313. During use, first, half-cover the film of the fixed cover plate 310 of the microfluidic chip, that is, expose the liquid injection channel 312 and the exhaust channel 313, inject the reaction reagent from the liquid injection channel 312, and the excess air will be discharged from the exhaust channel 313, so that the reagent is concentrated in the central reaction chamber. Then, perform full film covering. The double film covering design greatly avoids the pollution caused by the contact between the reaction liquid and the outside world. And because there is only one reaction chamber, there is no need to transfer liquids in multiple temperature zones, ensuring that the liquid will not remain in the microfluidic flow path and guaranteeing the integrity of the reagent.

[0062] In an alternative embodiment, the microfluidic chip assembly 300 further includes an electrode signal receiving end 350 and a microfluidic chip card seat 360; the electrode signal receiving end 350 is electrically connected to the electrode piece 320. After PCR amplification, the chemical signal is transmitted to the electrochemical detection board by the electrode piece 320 and the electrode signal receiving end 350 and converted into an electrical signal that is easy to process and detect. The microfluidic chip card seat 360 is arranged at the bottom of the microfluidic chip base 330, and the microfluidic chip card seat 360 has a card slot that can be used to clamp the microfluidic chip base 330.

[0063] For the electrochemical incubation detection module provided in this embodiment, since there is only one reaction chamber, only variable temperature control needs to be performed in one temperature zone, which reduces the occupied volume of the whole machine in space, increases convenience, and makes the whole machine more delicate; during the whole temperature control and temperature change process, high real-time responsiveness, high temperature control accuracy, and good stability can be achieved, improving the accuracy of electrochemical detection after amplification; since there is no liquid movement involved, that is, there is no risk of liquid in the moving pipeline, the loss of reaction reagents is reduced, and the availability is improved.

[0064] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An electrochemical incubation detection module, characterized in that, Comprising: a heat dissipation component (100), a heating component (200), and a microfluidic chip component (300); the microfluidic chip component (300) has a reaction chamber; the heating component (200) covers the microfluidic chip component (300), and the heating component (200) is used to heat the reaction chamber; the heat dissipation component (100) is disposed on top of the heating component (200), and the heat dissipation component (100) is configured to be able to dissipate and transfer the heat of the reaction chamber to cooperate with the heating component (200) to control the temperature of the reaction chamber.

2. The electrochemical incubation detection module according to claim 1, wherein the heat dissipation component (100) includes a heat dissipation fan (110) and a heat dissipation member (120); the heat dissipation member (120) is disposed on top of the heating component (200); the heat dissipation fan (110) is disposed on a side of the heat dissipation member (120) away from the heating component (200), and the heat dissipation fan (110) is used to transfer the heat in the reaction chamber.

3. The electrochemical incubation detection module according to claim 2, wherein the heat dissipation member (120) has a plurality of heat dissipation fins, and the plurality of heat dissipation fins are independently and spaced apart.

4. The electrochemical incubation detection module according to claim 1, wherein the microfluidic chip component (300) includes a microfluidic chip fixing cover plate (310), an electrode sheet (320), and a microfluidic chip base (330); the electrode sheet (320) is disposed on the microfluidic chip base (330); the microfluidic chip fixing cover plate (310) covers the electrode sheet (320), and the microfluidic chip fixing cover plate (310) is connected to the microfluidic chip base (330).

5. The electrochemical incubation detection module according to claim 4, wherein the microfluidic chip fixing cover plate (310) has a through hole (311), and a reaction chamber is formed by enclosing between the inner wall of the through hole (311) and the electrode sheet (320).

6. The electrochemical incubation detection module according to claim 5, wherein the microfluidic chip component (300) further includes a sealing ring (340); the sealing ring (340) is disposed in the through hole (311), and the sealing ring (340) is used to concentrate the reagent in the reaction chamber.

7. The electrochemical incubation detection module according to claim 6, wherein the microfluidic chip fixing cover plate (310) is provided with a liquid injection channel (312) and an exhaust channel (313); both the liquid injection channel (312) and the exhaust channel (313) are communicated with the through hole (311); the liquid injection channel (312) is used to inject liquid into the through hole (311), and the exhaust channel (313) is used to discharge the gas in the through hole (311).

8. The electrochemical incubation detection module according to claim 7, wherein The microfluidic chip assembly (300) further includes an electrode signal receiving end (350) and a microfluidic chip socket (360); The electrode signal receiving end (350) is electrically connected to the electrode sheet (320); The microfluidic chip socket (360) is disposed at the bottom of the microfluidic chip base (330), and the microfluidic chip socket (360) is used for clamping the microfluidic chip base (330).

9. The electrochemical incubation detection module according to claim 1, wherein The heating assembly (200) includes a connecting member and a heating sheet (230); The connecting member is connected to the heat dissipation assembly (100), and the connecting member is used to fix the heating sheet (230) so that the heating sheet (230) is located above the microfluidic chip assembly (300).

10. The electrochemical incubation detection module according to claim 9, wherein The connecting member includes a left fixing clip (210) and a right fixing clip (220); Both the left fixing clip (210) and the right fixing clip (220) are connected to the bottom of the heat dissipation assembly (100); Both sides of the heating sheet (230) are respectively lapped on the left fixing clip (210) and the right fixing clip (220).

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