Full-automatic non-interruption liquid preparation chromatography system

Abstract

The utility model provides a full-automatic non-interruption liquid preparation chromatography system and a use method thereof. The full-automatic non-interruption liquid preparation chromatography system comprises a first liquid inlet main pipeline, a pure water tank, an alkali liquor tank, a plurality of mother liquor tanks, a following tank, a product tank, a bubble trap, a plurality of chromatographic columns, a first liquid inlet main pipeline, a collecting tank and a waste discharge tank, the first liquid inlet main pipeline is sequentially connected with an outlet of the alkali liquor tank, an outlet of the pure water tank and outlets of the mother liquor tanks through pipelines respectively; an outlet pipeline of each mother liquor tank is connected with the first liquid inlet main pipeline through a connecting pipeline, and a mother liquor pump is arranged on each connecting pipeline; a main pump is arranged on the first liquid inlet main pipeline; the inlet end and the outlet end of the following tank are respectively connected with a first liquid inlet main pipeline through pipelines; the first liquid inlet main pipeline is connected with a product tank through a pipeline; the first liquid inlet main pipeline is respectively connected with an inlet end and an outlet end of bubble sinking through pipelines; the first liquid inlet main pipeline is connected with the inlet ends of the corresponding chromatographic columns through different pipelines, and the outlet ends of the chromatographic columns are connected with the first liquid inlet main pipeline through corresponding pipelines; the outlet end of the first liquid inlet main pipeline is respectively connected with a collecting tank and a waste discharge tank through valves; pumps are arranged on the first liquid inlet main pipeline, a connecting pipeline between each mother liquid tank and the first liquid inlet main pipeline and a connecting pipeline between the following tank and the first liquid inlet main pipeline; the utility model has the advantages that the problems of disqualified liquid preparation interruption and pressure change are solved, the working efficiency is improved, meanwhile, self-circulation can be realized, and quick formula conversion is realized after the target flow speed is achieved.

Description

Technical Field

[0001] The utility model relates to liquid preparation chromatography in the field of biopharmaceuticals, and particularly to a full-automatic uninterrupted liquid preparation chromatography system. Background Art

[0002] In the prior art, liquid preparation chromatography systems connect mother liquid tanks, buffer tanks, CIP, chromatography columns, and various valves, pumps, sensors, etc. together. First, the pump configures the mother liquid into each buffer tank according to the set ratio; then the solutions with different concentrations in the buffer tanks are conveyed to the chromatography column through a transfer pump as required for separation and purification. Its disadvantages are as follows: The traditional liquid preparation + chromatography operation is cumbersome; a large number of process buffer tanks need to be configured, occupying a large amount of space and being troublesome in the cleaning and sterilization process; the mother liquid needs to reach the set flow rate to enter the buffer tank, resulting in waste of resources; the instrument accuracy is poor, the signal is easily interfered and attenuated, leading to system instability, thus causing problems such as interruption of unqualified liquid preparation and pressure change.

[0003] At the same time, the pipeline flow paths currently adopted directly connect each liquid addition tank to the mixing tank, and its disadvantage is that the outlet pipelines on the connecting pipelines cannot be cleaned and cannot meet the hygiene requirements. Content of the Utility Model

[0004] In order to overcome the above deficiencies of the prior art, the utility model provides a full-automatic uninterrupted liquid preparation chromatography system and its usage method, which can realize the circulation of the mother liquid and the conversion of rapid formulation; can solve the problems of interruption and pressure change caused by unqualified liquid preparation;

[0005] In order to achieve the above purpose, the technical solution adopted by the utility model is as follows:

[0006] In view of the above technical problems, the first aspect of the disclosed content of the present utility model proposes a fully automatic non-stop liquid dispensing chromatography system, which includes a first main liquid inlet pipeline, a pure water tank, an alkali solution tank, a plurality of mother liquid tanks, a follow-up tank, a product tank, a bubble trap, a plurality of chromatography columns, a first main liquid inlet pipeline, a collection tank, and a waste discharge tank; the first main liquid inlet pipeline is sequentially connected to the outlet of the alkali solution tank, the outlet of the pure water tank, and the outlets of each mother liquid tank through pipelines; the outlet pipelines of each mother liquid tank are respectively connected to the first main liquid inlet pipeline through connecting pipelines, and mother liquid pumps are respectively arranged on the connecting pipelines; a main pump is arranged on the first main liquid inlet pipeline; the inlet end and the outlet end of the follow-up tank are respectively connected to the first main liquid inlet pipeline through pipelines; the first main liquid inlet pipeline is connected to the product tank through a pipeline; the first main liquid inlet pipeline is respectively connected to the inlet end and the outlet end of the bubble trap through pipelines; the first main liquid inlet pipeline is connected to the inlet ends of the corresponding respective chromatography columns through different pipelines, and the outlet ends of each chromatography column are connected to the first main liquid inlet pipeline through corresponding pipelines; the outlet end of the first main liquid inlet pipeline is respectively connected to the collection tank and the waste discharge tank through valves; pumps are arranged on the first main liquid inlet pipeline, the connecting pipelines between each mother liquid tank and the first main liquid inlet pipeline, and the connecting pipelines between the follow-up tank and the first main liquid inlet pipeline.

[0007] Further, the first main liquid inlet pipeline is connected to the inlet end of the alkali solution tank through a pipeline to realize the circulation of the alkali solution.

[0008] Further, the outlet pipeline of the alkali solution tank and the outlet pipeline of the pure water tank are connected to a second main liquid inlet pipeline, and the second main liquid inlet pipeline is respectively connected to the outlet ends of the outlet pipelines of each mother liquid tank.

[0009] Further, the outlet ends of the outlet pipelines of each mother liquid tank are respectively connected to a cleaning discharge tank.

[0010] Further, the bottom of the connecting pipeline between the outlet pipeline of each mother liquid tank and the first main liquid inlet pipeline is connected to the top inlet end of the connecting pipeline between the pipeline mother liquid tank and the first main liquid inlet pipeline through a bypass pipeline to form a closed loop.

[0011] Further, the outlet end of the bypass pipeline is connected to the waste discharge tank.

[0012] Further, a valve array is arranged on the first main liquid inlet pipeline, and the valve array is a rectangular valve array composed of four valves connected end to end. One pair of opposite sides of the rectangular valve array is connected to the first main liquid inlet pipeline; the other pair of opposite sides of the rectangular valve array is respectively connected to the upper and lower inlet and outlet ends of the chromatography column.

[0013] Further, the outlet of the alkali solution tank and the outlet pipeline of the pure water tank are connected to the first main liquid inlet pipeline through a connecting pipeline.

[0014] Further, the second main liquid inlet pipe is connected to the cleaning discharge pipe.

[0015] Further, the connections of the respective pipes are connected through valves.

[0016] Further, flow sensors are provided on the first main liquid inlet pipe, the connecting pipes between each mother liquid tank and the first main liquid inlet pipe, and the connecting pipe between the follow-up tank and the first main liquid inlet pipe.

[0017] In view of the above technical problems, the first aspect of the disclosed content of the present utility model provides a method for using a fully automatic non-stop Oh-eh chromatography system, and the steps are as follows:

[0018] Step 1: Preparing in the follow-up tank while loading the product; according to the formula, the solution and pure water in the corresponding mother liquid tank are transported to the follow-up tank through the first main liquid inlet pipe; at the same time, the product solution in the product tank enters the inner layer of the chromatography column through the bubble trap for chromatography and then is collected;

[0019] Step 2: Chromatography in the follow-up tank; the solution in the follow-up tank enters the inner layer of the chromatography column through the bubble trap for chromatography and then is collected;

[0020] Step 3: If different liquid preparations are required according to the formula, at this time, the solution in the mother liquid tank can be directly collected after chromatography through the chromatography column;

[0021] Step 4: Preparation of the lye, the preparation of the lye: the solution in the pure water tank and the solution in the corresponding mother liquid tank flow into the inlet end of the lye tank after passing through the first main liquid inlet pipe.

[0022] Step 5: Cleaning.

[0023] Further, the cleaning includes: (1) lye circulation; (2) pure water direct discharge; (3) cleaning of the follow-up tank pipeline.

[0024] Further, for the lye circulation: the lye flows out from the outlet of the lye tank, passes through the first main liquid inlet pipe, and then flows into the inlet of the lye tank; the lye passes through the second main liquid inlet pipe and flows into the connecting pipes between each mother liquid tank and the first main liquid inlet pipe respectively, and then enters the waste discharge tank through the bypass pipe, and also flows into the connecting pipeline between the first main liquid inlet pipe and the product tank outlet pipeline and the follow-up tank outlet pipeline, enters the first main liquid inlet pipe, and then enters the lye tank;

[0025] Further, for the pure water direct discharge: the pure water in the pure water tank flows through the first main liquid inlet pipe, the first main liquid inlet pipe, and then flows into the waste discharge pipe for collection and / or the pure water passes through the second main liquid inlet pipe and flows into the connecting pipes between each mother liquid tank and the first main liquid inlet pipe respectively, and then enters the waste discharge tank through the bypass pipe, and also flows into the connecting pipeline between the first main liquid inlet pipe and the product tank outlet pipeline and the follow-up tank outlet pipeline, enters the first main liquid inlet pipe, and then enters the waste discharge tank;

[0026] Further, for the cleaning of the follow-up tank pipeline: the lye flows into the inlet end of the follow-up tank through the first main liquid inlet pipeline, and then flows into the inlet of the lye tank through the outlet end of the follow-up tank and the first main liquid inlet pipeline; the pure water flows into the inlet end of the follow-up tank through the first main liquid inlet pipeline, and then flows into the waste discharge pipeline through the outlet end of the follow-up tank and the first main liquid inlet pipeline.

[0027] Advantages of the present utility model:

[0028] 1. A follow-up tank is provided, which can realize the preparation of liquid to the follow-up tank; from the follow-up tank to the chromatography column, it can realize the interruption of unqualified liquid preparation and the problem of pressure change;

[0029] 2. Adding a cleaning lye tank can enable multiple chromatography columns to work staggeredly, that is, some are for chromatography while some are for cleaning, improving the work efficiency.

[0030] 3. The addition of the mother liquor pump can realize self-cleaning and defoaming; at the same time, it can realize self-circulation, and quickly switch the formula after reaching the target flow rate. Description of the Drawings

[0031] Figure 1 It is a structural schematic diagram of the present utility model. Detailed Embodiments

[0032] The following further describes the present utility model with reference to the drawings.

[0033] The following specific embodiments illustrate the technical content of the present utility model. Those skilled in the art can easily understand other advantages and effects of the present utility model from the content disclosed in this specification. The present utility model can also be implemented or applied through other different specific embodiments. Those skilled in the art make various modifications and changes without departing from the spirit of the present utility model.

[0034] Before introducing the specific embodiments of the present disclosure in detail, some terms used in the present disclosure are first explained.

[0035] Unless otherwise defined hereinafter, the meanings of all technical terms and scientific terms used herein are intended to be the same as those commonly understood by those skilled in the art. References to techniques used herein refer to techniques commonly understood in the art, including variations of those techniques that are obvious to those skilled in the art or substitutions of equivalent techniques. Although it is believed that the following terms are well understood by those skilled in the art, the following definitions are still set forth to better explain the present utility model. When a trade name appears herein, it is intended to refer to its corresponding product. All patents, published patent applications, and publications cited herein are incorporated herein by reference.

[0036] Unless otherwise specified in the text, multiple referents such as "a", "the" include plural referents. The expressions "one or more" or "at least one" can mean 1, 2, 3, 4, 5, 6, 7, 8, 9 or more.

[0037] As used herein, terms such as "connected", "linked", "coupled" or "coupling" and similar words are not limited to direct connection, but also include indirect connection.

[0038] As described herein, "sample" refers to biomolecules, including proteins, nucleic acids, lipids, carbohydrates, oligonucleotides, amino acids and their derivatives.

[0039] As used herein, the terms "online monitoring" or "real-time monitoring" refer to the real-time detection of certain parameters or properties of buffer, reaction fluid, fluid flowing out of the flow reactor during the use of the chromatography system, such as pH value, pressure, flow rate, conductivity, etc. Different from offline detection or analysis, online monitoring or real-time monitoring can provide real-time feedback of the detection results.

[0040] As used herein, the positional relationship words such as "above", "below", "left", "right", "front", "rear", etc. are determined according to the layout direction of the specification drawings, and they are only used to represent the relative positional relationship. When the absolute position of the object being described changes, the relative positional relationship may also change accordingly.

[0041] Refer to Figure 1As shown in the figure, this embodiment uses a chromatography column and five mother liquor tanks. A fully automatic non-stop liquid dispensing chromatography system includes an alkali liquor storage tank 1, a pure water tank 2, a mother liquor A tank 3, a mother liquor B tank 4, a mother liquor C tank 5, a mother liquor D tank 6, a mother liquor H tank 7, a follow-up tank 8 and a product tank 9. The outlet ends of the outlet pipelines of the pure water tank 2 and the alkali liquor storage tank 1 are respectively connected to the inlet end of the first main liquid inlet pipeline 10 and the inlet end of the second main liquid inlet pipeline 11. Valves are provided on the outlet pipeline of the pure water tank 2 and the inlet end of the first main liquid inlet pipeline 10; a valve is provided on the outlet pipeline of the alkali liquor storage tank 1; the mother liquor A tank 3, the mother liquor B tank 4, the mother liquor C tank 5, the mother liquor D tank 6, and the mother liquor H tank 7 are arranged in sequence from left to right. The outlet pipeline of the mother liquor A tank 3 is connected to the first main liquid inlet pipeline 10 through a first connecting pipeline 12 by a valve. A valve is provided on the outlet pipeline of the mother liquor A tank 3, and a valve is provided at the liquid inlet end of the first connecting pipeline 12. The outlet pipeline of the mother liquor A tank 3 is connected to the cleaning discharge tank 24 through a valve. The first connecting pipeline 12 is provided with a first mother liquor pump 13 and a first flow sensor 14. The bottom of the first connecting pipeline 12 is connected to the inlet end of a first bypass pipeline 15 through a valve. The outlet end of the first bypass pipeline 15 is respectively connected to the top inlet end of the first connecting pipeline 12 and the waste discharge tank 16 through valves; and so on for the mother liquor B tank 4, the mother liquor C tank 5, the mother liquor D tank 6, and the mother liquor H tank 7. The mother liquor A tank 3 is connected to the first main liquid inlet pipeline 10 in the above-mentioned sequential manner. A first liquid inlet pump 17 is provided on the first main liquid inlet pipeline 10. The liquid inlet pump 17 is located on the right side of the connection point between the mother liquor H tank 7 and the first main liquid inlet pipeline 10. The first main liquid inlet pipeline 10 is also respectively provided with a second flow sensor 18, a first pressure sensor 19, a first conductivity transmitter 20 and a first pH sensor 21; the first main liquid inlet pipeline 10 is connected to the inlet end of the follow-up tank 22 through a connecting pipeline; the outlet pipelines of the product tank 23, the follow-up tank 22 and the cleaning discharge tank 24 are respectively connected to the inlet end of a second connecting pipeline 26 through valves. The outlet end of the second connecting pipeline 26 is connected to the first main liquid inlet pipeline 10 through a valve. The second connecting pipeline 26 is respectively provided with a second liquid inlet pump 27 and a third flowmeter 28. The second main liquid inlet pipeline 11 is respectively connected to the outlet pipelines of the mother liquor A tank 3, the mother liquor B tank 4, the mother liquor C tank 5, the mother liquor D tank 6, and the mother liquor H tank 7 through connecting pipelines. Valves are respectively provided on the connecting pipelines to control the on-off; and the second main liquid inlet pipeline 11 is connected to the outlet pipeline of the cleaning discharge tank 24. The first main liquid inlet pipeline 10 is respectively connected to the inlet end and the outlet end of a bubble trap 29 through a connecting pipeline. Valves are respectively provided on the connecting pipeline and on the first main liquid inlet pipeline 10 between the inlet and outlet ends. The connection point between the bubble trap 29 and the first main liquid inlet pipeline 10 is located on the right side of the connection point between the second connecting pipeline 26 and the first main liquid inlet pipeline 10.A valve array is provided on the first main liquid inlet pipe 10. The valve array is a rectangular valve array composed of four valves connected end to end. One pair of opposite sides of the rectangular valve array is connected to the first main liquid inlet pipe 10, and there are 10 valves provided on the first main liquid inlet pipe. The other pair of opposite sides of the rectangular valve array are respectively connected to the upper and lower inlets and outlets of the chromatography column 30. A UV sensor 31, a second conductivity transmitter 32, and a second pH sensor 33 are respectively provided on the first main liquid inlet pipe 10 on the right side of the rectangular valve array. The outlet end of the first main liquid inlet pipe 10 is respectively connected to the inlet end of the alkali liquid storage tank 1, the inlet of the collection tank 34, and the inlet of the waste discharge tank 16 through connecting pipes, and valves are respectively provided on the connecting pipes. The connection point between the follow-up tank 22 and the first main liquid inlet pipe 10 is connected to the inlet of the waste discharge tank 16 through a connecting pipe, and a valve is provided on the connecting pipe. The connection point between the outlet end of the bubble trap 29 and the first main liquid inlet pipe 10 is connected to the inlet of the waste discharge tank 16 through a connecting pipe, and the top outlet of the bubble trap 29 is connected to the inlet of the waste discharge tank 16 through a connecting pipe, and valves are respectively provided on the connecting pipes. A bubble sensor 35 and a second pressure sensor 36 are provided on the first main liquid inlet pipe 10 between the bubble trap 29 and the rectangular valve array.

[0042] During use: First: Product loading and pre - preparation in the follow - up tank (1) When loading the product, the valve on the outlet pipeline of the product tank 23 is opened, and the second liquid inlet pump 27 on the second connecting pipe 26 is opened. The product liquid to be processed passes through the first main liquid inlet pipe 10, the bubble trap 29, and the rectangular valve array group and enters the chromatography column 30. The processed product liquid is discharged into the waste discharge tank 16 for collection after passing through the first main liquid inlet pipe 10 from the outlet of the chromatography column 30. (2) Pre - preparation in the follow - up tank. For example, when preparing buffer A, the first liquid inlet pump 17 on the first main liquid inlet pipe 10 is opened at this time, and the first mother liquid pump 13 on the first connecting pipe 12 connected to the mother liquid tank is also opened. For example, mother liquid tank A 3, mother liquid tank C 5, and mother liquid tank D 6. At this time, the solution in the pure water tank 2, the solutions in mother liquid tank A 3, mother liquid tank C 5, and mother liquid tank D 6 all enter the first main liquid inlet pipe 10. The second flow sensor 18, the first pressure sensor 19, the first conductivity transmitter 20, and the first pH sensor 21 monitor the data in real - time. The unqualified buffer solution in the early stage will enter the waste discharge tank 16 through the connecting pipe at the connection point between the follow - up tank 22 and the first main liquid inlet pipe 10; when the buffer solution is qualified, the valve on the connecting pipe connecting to the waste discharge tank 16 is closed at this time, and the valve on the connecting pipe connecting the first main liquid inlet pipe 10 and the inlet of the follow - up tank 22 is opened at this time, and buffer A will enter the follow - up tank 22.

[0043] II: Follow-up tank chromatography. When the chromatography of the product liquid is completed and elution is required, buffer A is used as the eluent at this time. Then, the valve of the outlet pipeline of the follow-up tank 22 is opened, and the buffer solution in the follow-up tank 22 passes through the second connecting pipeline 26, the bubble trap 29, and the rectangular valve array group and enters the chromatography column 30. After eluting the product adsorbed in the chromatography column 30, it is discharged into the collection tank 34 through the first main liquid inlet pipeline 10.

[0044] III. If different liquid preparations are required according to the formula, the solution in the mother liquor tank can be directly collected after chromatography through the chromatography column: for example, when cleaning is required and buffer B needs to be prepared, the first liquid inlet pump 17 on the first main liquid inlet pipeline 10 is opened, and at the same time, the first mother liquor pump 13 on the first connecting pipeline 12 communicating with the mother liquor tank is opened, such as the mother liquor C tank 5 and the mother liquor H tank 7; at this time, the solution in the pure water tank 2, the solutions in the mother liquor C tank 5 and the mother liquor H tank 7 all enter the first main liquid inlet pipeline 10, and the second flow sensor 18, the first pressure sensor 19, the first conductivity transmitter 20, and the first pH sensor 21 monitor the data in real time. After passing through the bubble trap 29 and the shaped valve array group, they enter the chromatography column 30 and are collected in the waste discharge tank 16 after flowing out from the outlet of the chromatography column 30.

[0045] IV. Preparation of lye. When lye needs to be prepared, the first liquid inlet pump 17 on the first main liquid inlet pipeline 10 is opened at this time, and at the same time, the first mother liquor pump 13 on the first connecting pipeline 12 communicating with the mother liquor tank is opened, for example: the mother liquor D tank 6. At this time, the solution in the pure water tank 2 and the solution in the mother liquor D tank 6 both enter the first main liquid inlet pipeline 10, and the second flow sensor 18, the first pressure sensor 19, the first conductivity transmitter 20, and the first pH sensor 21 monitor the data in real time. Then, it flows into the inlet end of the lye storage tank 1 through the outlet end of the first main liquid inlet pipeline 10 and enters the lye storage tank 1.

[0046] V. Cleaning

[0047] (1) Lye circulation:

[0048] At this time, the first liquid inlet pump 17 on the first main liquid inlet pipeline 10, the first mother liquor pump 13 on the first connecting pipeline 12, and the second liquid inlet pump 27 on the second connecting pipeline 26 are respectively opened, and lye enters the first main liquid inlet pipeline 10 and the second main liquid inlet pipeline 11 respectively; part of the lye in the second main liquid inlet pipeline 11 enters the first connecting pipeline 12 of each mother liquor tank and the first bypass pipeline 15 communicating therewith through the second main liquid inlet pipeline 11 and then enters the waste discharge tank 16, and another part of the lye enters the lye storage tank 1 after passing through the second connecting pipeline 26 and entering the first main liquid inlet pipeline 10; the lye directly entering the first main liquid inlet pipeline 10 enters the lye storage tank 1 along the first main liquid inlet pipeline 10.

[0049] (2) Direct discharge of pure water

[0050] At this time, the first liquid inlet main pump 17 on the first liquid inlet main pipeline 10, the first mother liquid pump 13 on the first connection pipeline 12, and the second liquid inlet pump 27 on the second connection pipeline 26 are respectively opened, and pure water enters the first liquid inlet main pipeline 10 and the second liquid inlet main pipeline 11 respectively; part of the pure water in the second liquid inlet main pipeline 11 enters the first connection pipeline 12 of each mother liquid tank through the second liquid inlet main pipeline 11 and enters the waste discharge tank 16 through the first bypass pipeline 15. There is also a part of the lye that enters the first liquid inlet main pipeline 10 through the second connection pipeline 26 and then enters the waste discharge tank 16; the lye that directly enters the first liquid inlet main pipeline 10 enters the waste discharge tank 16 along the first liquid inlet main pipeline 10.

[0051] (3) Follow the cleaning of the tank pipeline

[0052] Open the first liquid inlet pump 17 on the first liquid inlet main pipeline 10. The alkaline water in the alkaline liquid storage tank 1 enters the follow-up tank 22 through the first liquid inlet main pipeline 10, then flows out from the outlet of the follow-up tank 22 to the first liquid inlet main pipeline 10, and finally returns to the alkaline liquid storage tank 1; open the first liquid inlet pump 17 on the first liquid inlet main pipeline 10. The pure water in the pure water tank 2 enters the follow-up tank 22 through the first liquid inlet main pipeline 10, then flows out from the outlet of the follow-up tank 22 to the first liquid inlet main pipeline 10, and finally returns to the waste discharge tank 16.

Claims

1. An automatic uninterrupted liquid dispensing chromatography system, characterized in that, Including a first main liquid inlet pipeline, a pure water tank, an alkali solution tank, multiple mother liquid tanks, a follow-up tank, a product tank, a bubble trap, multiple chromatography columns, a first main liquid inlet pipeline, a collection tank, and a waste discharge tank; the first main liquid inlet pipeline is sequentially connected to the outlet of the alkali solution tank, the outlet of the pure water tank, and the outlets of each mother liquid tank through pipelines; the outlet pipelines of each mother liquid tank are respectively connected to the first main liquid inlet pipeline through connecting pipelines, and mother liquid pumps are respectively arranged on the connecting pipelines; a main pump is arranged on the first main liquid inlet pipeline; the inlet end and the outlet end of the follow-up tank are respectively connected to the first main liquid inlet pipeline through pipelines; the first main liquid inlet pipeline is connected to the product tank through a pipeline; the first main liquid inlet pipeline is respectively connected to the inlet end and the outlet end of the bubble trap through pipelines; the first main liquid inlet pipeline is connected to the inlet ends of the corresponding respective chromatography columns through different pipelines, and the outlet ends of each chromatography column are connected to the first main liquid inlet pipeline through corresponding pipelines; the outlet end of the first main liquid inlet pipeline is respectively connected to the collection tank and the waste discharge tank through valves; pumps are arranged on the first main liquid inlet pipeline, the connecting pipelines between each mother liquid tank and the first main liquid inlet pipeline, and the connecting pipelines between the follow-up tank and the first main liquid inlet pipeline.

2. The fully automatic uninterrupted liquid dispensing and chromatography system according to claim 1, wherein, The first main liquid inlet pipeline is connected to the inlet end of the alkali solution tank through a pipeline to realize the circulation of the alkali solution.

3. A fully automatic uninterrupted liquid dispensing chromatography system according to claim 1, characterized in that, The outlet pipeline of the alkali solution tank and the outlet pipeline of the pure water tank are communicated with a second main liquid inlet pipeline, and the second main liquid inlet pipeline is respectively communicated with the outlet ends of the outlet pipelines of each mother liquid tank.

4. The fully automatic uninterrupted liquid dispensing chromatography system according to claim 3, wherein The second main liquid inlet pipeline is connected to a cleaning discharge pipeline.

5. The full-automatic uninterrupted liquid dispensing chromatography system according to claim 1, wherein The outlet ends of the outlet pipelines of each mother liquid tank are respectively connected to a cleaning discharge tank.

6. The fully automatic uninterrupted liquid dispensing chromatography system according to claim 1, wherein The bottom of the connecting pipeline between the outlet pipeline of each mother liquid tank and the first main liquid inlet pipeline is communicated with the top inlet end of the connecting pipeline between the pipeline mother liquid tank and the first main liquid inlet pipeline through a bypass pipeline to form a closed loop.

7. The fully automatic non-interrupted liquid dispensing chromatography system according to claim 6, wherein The outlet end of the bypass pipeline is connected to the waste discharge tank.

8. The fully automatic uninterrupted liquid dispensing chromatography system according to claim 1, wherein, A valve array is arranged on the first main liquid inlet pipeline. The valve array is a rectangular valve array composed of four valves connected end to end. One pair of opposite sides of the rectangular valve array is communicated with the first main liquid inlet pipeline; the other pair of opposite sides of the rectangular valve array is respectively communicated with the upper inlet and outlet and the lower inlet and outlet of the chromatography column.

9. The fully automatic uninterrupted liquid dispensing chromatography system according to claim 1, wherein The outlet of the liquid tank and the outlet pipeline of the pure water tank are connected to the first main liquid inlet pipeline through a connecting pipeline.

10. A fully automatic non-stop liquid dispensing chromatography system according to claim 1, characterized in that, The joints of each pipeline are connected through valves.

11. A fully automatic non-interruptive liquid dispensing chromatography system according to claim 1, characterized in that, Flow sensors are arranged on the first main liquid inlet pipeline, the connecting pipelines between each mother liquid tank and the first main liquid inlet pipeline, and the connecting pipelines between the follow-up tank and the first main liquid inlet pipeline.

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