Pipetting workbench

By designing a centralized modular pipetting workbench, the inefficiency and error-prone problems caused by the messy placement of reagents and equipment in the prior art are solved, and an efficient test and detection process is achieved.

CN223128095UActive Publication Date: 2025-07-22FUZHOU XINBEI BIOCHEM IND
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Patent Information

Application Number
CN202422187526.7
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-09-06
Publication Date
2025-07-22
Estimated Expiration
2034-09-06

AI Technical Summary

Technical Problem

The messy placement of existing pipetting equipment and reagents leads to low testing efficiency and error-prone problems.

Method used

A centralized and modular pipetting workbench is designed, including a sample rack, a heating shock, a waste tube box, a suction tube positioning structure, a reagent placement table and an enzyme label plate placement rack. The structure is simple and convenient to use, and can effectively place reagents and equipment in a centralized manner to improve the testing and detection efficiency.

Benefits of technology

By heating and oscillating the sample liquid on the heating oscillator and mixing it evenly, and transferring the processed liquid to the enzyme plate for detection, the test detection efficiency is significantly improved and the occurrence of errors is reduced.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to a pipetting workbench which comprises a machine table, and a sample rack, a heating oscillator, a waste pipe box, a pipette positioning structure, a reagent placing table and an elisa plate placing rack which are arranged on the surface of the machine table, and a plurality of grooves for placing reagent bottles are formed in the surface of one side of the reagent placing table; a main reagent fixing frame for placing a main reagent is arranged on the surface of the other side of the reagent placing table, the sample frame is used for placing a test tube A filled with a sample, a test tube B is placed on the heating oscillator, the pipette positioning structure is used for placing a pipette, and the elisa plate placing frame is used for placing an elisa plate. The device is simple in structure, reasonable in design and convenient to use, sample detection is facilitated by reasonably arranging the centralized and modularized pipetting detection workbench, and the detection efficiency of the sample is improved.
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Description

Technical Field

[0001] The utility model relates to a pipetting workbench. Background Art

[0002] Pipetting is widely used in genomics research such as nucleic acid extraction, construction of various biological laboratory systems, large-scale sequencing, drug screening, protein analysis, etc. During the pipetting process, a pipette is usually used for pipetting work. After each pipetting, the pipette tip on the pipette needs to be replaced to prevent cross-contamination of different reagents and avoid experimental errors. In the prior art, some reagents needed are usually placed in a messy manner, and some equipment required for experiments is also placed separately in different places. This causes the inability to quickly proceed with the next experiment after each replacement of the pipette tip, resulting in low experimental detection efficiency and easy errors. Therefore, a centralized and modular pipetting workbench is designed. Summary of the Utility Model

[0003] The utility model improves the above problems, that is, the technical problem to be solved by the utility model is to provide a pipetting workbench with a simple structure and convenient use.

[0004] The utility model is composed of a machine table and a sample rack, a heating shaker, a waste tube box, a pipette tip positioning structure, a reagent placement table, and an ELISA plate placement rack arranged on the surface of the machine table. A plurality of grooves for placing reagent bottles are arranged on one side surface of the reagent placement table, and a main reagent fixing rack for placing the main reagent is arranged on the other side surface of the reagent placement table. The sample rack is used to place test tube A containing samples, test tube B is placed on the heating shaker, the pipette tip positioning structure is used to place pipette tips, and the ELISA plate placement rack is used to place ELISA plates.

[0005] Further, the sample rack includes a top plate and a bottom plate arranged at intervals. The front and rear parts of the top plate are connected by vertical plates. The space between the top plate and the bottom plate is a hollow part. Through holes are opened on the top plate, and slot openings are opened on the surface of the bottom plate. Each slot opening corresponds to a through hole, and the corresponding slot opening and through hole are used to place test tube A.

[0006] Further, a waste tube placement opening is opened on the surface of the waste tube box.

[0007] Further, the pipette tip positioning structure includes a positioning seat, a fixed block and a movable pressing block respectively arranged on both sides above the positioning seat. The movable pressing block is fixed on the positioning seat through a rotating shaft, and a spring is arranged between the positioning seat and the movable pressing block; a clamping groove is arranged inside the fixed block; a fixed column is arranged behind the fixed block, and a column cap is arranged above the fixed column; the upper part of the positioning seat is open, and a cavity is arranged in the middle of the positioning seat; an installation groove for installing the movable pressing block is arranged on one side of the positioning seat.

[0008] Further, a pipetting fixing plate is mounted on the surface of the positioning seat. A plurality of through holes for placing pipette tubes are formed in the pipetting fixing plate. U-shaped clamping grooves are arranged on both sides of the pipetting fixing plate. One of the U-shaped clamping grooves cooperates with a fixing block on one side of the positioning seat, and the other U-shaped clamping groove cooperates with a movable pressing block on the other side of the positioning seat. A semi-circular groove is further formed on one side of the pipetting fixing plate, and the semi-circular groove abuts against a fixing column of the positioning seat.

[0009] Further, the ELISA plate placement rack includes an upper plate and U-shaped side plates arranged on both sides of the upper plate. The upper plate and the two U-shaped side plates form a limiting space for placing ELISA plates. A lower plate is arranged below the upper plate, and the upper plate and the lower plate are connected by two support plates.

[0010] Further, notches are provided at the front and rear parts of the upper plate.

[0011] Further, the main reagent fixing rack is in a "mouth" shape, and a main reagent fixing opening for placing main reagent test tubes is arranged at the upper part of the main reagent fixing rack.

[0012] Compared with the prior art, the utility model has the following beneficial effects: The device has a simple structure, reasonable design, is convenient and efficient to use, improves the test detection efficiency, centrally modularizes and places the equipment and reagents required for the test. By placing the sample liquid in test tube A of the sample rack, first move the liquid in test tube A to test tube B on two of the heating shakers, then add the reagents placed on the reagent placement table into test tube B as required for heating and shaking for mixing, then transfer the liquid in test tube B on the two heating shakers to test tube B on the other two heating shakers, add the main reagent and heat and shake for mixing, and at the same time add the required reagents to test tube B on the other two heating shakers and shake for mixing; then move the processed liquid in test tube B to the ELISA plate, and finally detect the liquid in the ELISA plate. Description of the Drawings

[0013] Figure 1 It is a top view of an embodiment of the utility model;

[0014] Figure 2 It is a top view of the pipette tube positioning structure of an embodiment of the utility model;

[0015] Figure 3 It is a cross-sectional view of the sample rack of an embodiment of the utility model;

[0016] Figure 4 It is a cross-sectional view of the ELISA plate placement rack of an embodiment of the utility model;

[0017] Figure 5 It is a cross-sectional view of the main reagent fixing rack of an embodiment of the utility model;

[0018] Figure 6 Cross-sectional view of the liquid suction tube positioning structure according to an embodiment of the present utility model;

[0019] Figure 7 Stereogram of the liquid suction tube positioning structure according to an embodiment of the present utility model;

[0020] Figure 8 Top view of the pipetting fixing plate according to an embodiment of the present utility model. Specific implementation manner

[0021] The present utility model will be further described in detail below with reference to the accompanying drawings and specific implementation manners.

[0022] Embodiment 1: As shown in the attached Figure 1-8 figure, in this embodiment, a pipetting workbench is provided, which includes a machine table 7 and a sample rack 1, four heating shakers 2, a waste tube box 3, two liquid suction tube positioning structures 4, a reagent placement table 5, and an ELISA plate placement rack 6 arranged on the surface of the machine table. A plurality of grooves 501 for placing reagent bottles 504 are arranged on one side surface of the reagent placement table, and a main reagent fixing rack 502 for placing the main reagent is arranged on the other side surface of the reagent placement table; the sample rack is used to place test tubes A101 containing samples, a placement opening 202 is arranged on the heating shaker, and this placement opening can be used to place test tubes B201. The liquid suction tube positioning structure 4 is used to place the liquid suction tube 414, and the ELISA plate placement rack is used to place the ELISA plate 601.

[0023] The above-mentioned heating shaker is a prior art, which is used to heat and shake the test tube B placed on the test tube hole of the heating shaker, so as to realize the heating and shaking mixing of the sample and the added liquid medicine; the above-mentioned heating shaker can adopt a test tube heating shaker with the model TS100 manufactured by Hangzhou Ruicheng Instrument Co., Ltd.

[0024] The reagents placed in the grooves of the above-mentioned reagent placement table can be serum treatment liquid, alkali reagent, buffer solution, diazotization reagent, etc.

[0025] In the embodiment of the present utility model, the sample rack 1 includes a top plate 102 and a bottom plate 103 arranged at intervals. The front and rear parts of the top plate are both connected by vertical plates 104. The space between the top plate and the bottom plate is a hollow part 105. A plurality of through openings 106 are opened on the top plate, and a plurality of slot openings 107 are opened on the surface of the bottom plate. Each slot opening corresponds to a through opening. Each upper and lower corresponding slot opening and through opening are used to place the test tube A, and the sample is placed in the test tube A; the sample can be centrifuged blood.

[0026] In an embodiment of the present utility model, in order to facilitate the recycling of the used waste pipette tips, a waste tip placement opening 301 is provided on the surface of the waste tip box 3.

[0027] In an embodiment of the present utility model, the ELISA plate placement rack 6 includes an upper plate 602 and U-shaped side plates 603 provided on both sides of the upper plate. A limiting space 604 for placing the ELISA plate is formed between the upper plate and the two U-shaped side plates. A lower plate 605 is provided below the upper plate, and the upper plate and the lower plate are connected by two support plates 606.

[0028] In order to facilitate the taking and placing of the ELISA plate, notches 607 are provided at the front and rear parts of the upper plate.

[0029] In an embodiment of the present utility model, the main reagent fixing rack 5 is in a "mouth" shape, and at least two main reagent fixing openings 503 for placing the main reagent test tubes 505 are provided on the upper part of the main reagent fixing rack.

[0030] In an embodiment of the present utility model, during use: First, place the sample liquid in test tube A of the sample rack. First, move the liquid in test tube A to test tube B on two of the heating shakers. Then, add the reagents placed on the reagent placement table into test tube B as required for heating and shaking for mixing. Then, transfer the liquid in test tube B on the two heating shakers to test tube B on the other two heating shakers, add the main reagent and heat and shake for mixing. At the same time, add the required reagents to test tube B on the other two heating shakers and shake for mixing. Then, move the processed liquid in test tube B to the ELISA plate, and finally detect the liquid in the ELISA plate.

[0031] Each time when sucking the liquid medicine with a pipette as described above, it is necessary to replace the pipette tip. The new pipette tip is taken from the pipette fixing plate, and the used pipette tip can be discarded in the waste tip box.

[0032] Embodiment 2: On the basis of Embodiment 1, in this embodiment, the pipette tip positioning structure 4 includes a positioning seat 401, a fixing block 402 and a movable pressing block 403 respectively provided on both sides above the positioning seat. The movable pressing block is fixed to the positioning seat by a rotating shaft 404, and a spring 405 is provided between the positioning seat and the movable pressing block.

[0033] The pressing part 406 of the above-mentioned movable pressing block extends above the inner cavity of the positioning seat, and under the elastic force of the spring, the pressing part always maintains a downward pressing state.

[0034] A clamping groove 407 is provided inside the fixing block, and the cross section of the clamping groove can be arc-shaped; the cross section of the above-mentioned movable pressing block is hook-shaped, and the contact surface of the pressing part with the pipette fixing plate can be a plane.

[0035] In the embodiment of the present utility model, a fixing column 408 is provided behind the fixing block, and a column cap 409 is provided above the fixing column.

[0036] In the embodiment of the present utility model, the upper part of the positioning seat is open, and a cavity 411 is provided in the middle of the positioning seat.

[0037] In the embodiment of the present utility model, an installation groove 412 for installing a movable pressing block is provided on one side of the positioning seat; the rotating shaft and the spring are arranged in the installation groove.

[0038] In the embodiment of the present utility model, a pipetting fixing plate 413 is installed on the surface of the positioning seat. A plurality of through holes 415 for placing pipette tubes 414 are provided on the pipetting fixing plate. U-shaped clamping grooves 416 are provided on both sides of the pipetting fixing plate. One of the U-shaped clamping grooves cooperates with the fixing block on one side of the positioning seat, and the other U-shaped clamping groove cooperates with the movable pressing block on the other side of the positioning seat.

[0039] In the embodiment of the present utility model, a semi-circular groove 417 is further provided on one side of the pipetting fixing plate, and the semi-circular groove abuts against the fixing column of the positioning seat.

[0040] Vertical plates 418 are provided on the front and rear sides of the surface of the above-mentioned pipetting fixing plate.

[0041] In the embodiment of the present utility model, during installation: align and abut the clamping groove on the left side of the pipetting fixing plate with multiple pipette tubes against the fixing block on the left side of the positioning seat. At the same time, the semi-circular groove on the left side of the pipetting fixing plate also abuts against the fixing column. Press the lower part of the movable pressing block to make the upper part of the movable pressing block rotate counterclockwise to the right along the rotating shaft. The pressing part of the movable pressing block leaves the surface of the positioning seat. Then swing the right side of the pipetting fixing plate downward to contact the right side surface of the positioning seat. Release the movable pressing block, and the movable pressing block presses the edge part located in the U-shaped clamping groove on the right side of the pipetting fixing plate, thereby limiting the pipetting fixing plate to facilitate the subsequent direct insertion of the washing gun into the pipette tube on the pipetting fixing plate for use.

[0042] For any of the technical solutions disclosed in the above-mentioned present utility model, unless otherwise stated, if it discloses a numerical range, the disclosed numerical range is a preferred numerical range. Any person skilled in the art should understand that the preferred numerical range is only the numerical values with obvious technical effects or representativeness among many implementable numerical values. Since there are too many numerical values to enumerate, only some numerical values are disclosed in the present utility model to illustrate the technical solutions of the present utility model. Moreover, the above-listed numerical values should not constitute a limitation on the protection scope of the present invention.

[0043] If terms such as "first" and "second" are used in this article to define components, those skilled in the art should be aware that the use of "first" and "second" is only for the convenience of distinguishing components in description. Unless otherwise stated, the above terms have no special meaning.

[0044] Meanwhile, if the above-mentioned utility model discloses or involves components or structural parts that are fixedly connected to each other, then, unless otherwise stated, the fixed connection can be understood as: a detachable fixed connection (for example, connected by bolts or screws), or it can also be understood as: a non-detachable fixed connection (for example, riveting, welding). Of course, the mutual fixed connection can also be replaced by an integral structure (for example, manufactured by integral forming using casting technology) (except where it is clearly impossible to use the integral forming process).

[0045] In addition, in any technical solution disclosed by the above-mentioned utility model, the terms used to represent the positional relationship or shape, unless otherwise stated, include states or shapes that are approximate, similar, or close to it.

[0046] Any component provided by the present utility model can either be assembled from multiple separate components or be a single component manufactured by integral forming technology.

[0047] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present utility model and not to limit them; although the present utility model has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: it is still possible to modify the specific implementation manners of the present utility model or make equivalent replacements for some technical features; without departing from the spirit of the technical solutions of the present utility model, they should all be covered within the scope of the technical solutions claimed by the present utility model.

Claims

1. A pipetting workbench, characterized in that, It includes a machine platform, as well as a sample rack, a heating shaker, a waste tube box, a pipette positioning structure, a reagent placement table, and an ELISA plate placement rack arranged on the surface of the machine platform. On one side surface of the reagent placement table, there are a plurality of grooves for placing reagent bottles. On the other side surface of the reagent placement table, there is a main reagent fixing rack for placing the main reagent. The sample rack is used for placing test tube A containing a sample. There is a test tube B placed on the heating shaker. The pipette positioning structure is used for placing pipettes. The ELISA plate placement rack is used for placing ELISA plates.

2. The pipetting workbench according to claim 1, characterized in that, The sample rack includes a top plate and a bottom plate arranged at intervals. The front and rear parts of the top plate are connected by vertical plates. The space between the top plate and the bottom plate is a hollow part. There is a through hole opened on the top plate, and a notch is opened on the surface of the bottom plate. Each notch corresponds to the through hole, and the corresponding notch and through hole are used for placing test tube A.

3. The pipetting workbench according to claim 1, characterized in that, The surface of the waste tube box is provided with a waste tube placement opening.

4. The pipetting workbench according to claim 1, characterized in that, The pipette positioning structure includes a positioning seat, a fixed block and a movable pressing block respectively arranged on both sides above the positioning seat. The movable pressing block is fixed on the positioning seat through a rotating shaft, and a spring is arranged between the positioning seat and the movable pressing block; a clamping groove is arranged inside the fixed block; a fixed column is arranged behind the fixed block, and a column cap is arranged above the fixed column; the upper part of the positioning seat is in an open shape, and a cavity is arranged in the middle of the positioning seat; there is an installation groove for installing the movable pressing block on one side of the positioning seat.

5. The pipetting workbench according to claim 4, wherein A pipetting fixing plate is installed on the surface of the positioning seat. A plurality of through holes for placing pipettes are opened on the pipetting fixing plate. U-shaped clamping grooves are arranged on both sides of the pipetting fixing plate. One of the U-shaped clamping grooves cooperates with the fixed block on one side of the positioning seat, and the other U-shaped clamping groove cooperates with the movable pressing block on the other side of the positioning seat; a semi-circular groove is also opened on one side of the pipetting fixing plate, and the semi-circular groove abuts against the fixed column of the positioning seat.

6. The pipetting workbench according to claim 1, characterized in that, The ELISA plate placement rack includes an upper plate and U-shaped side plates arranged on both sides of the upper plate. The upper plate and the two U-shaped side plates form a limiting space for placing ELISA plates. A lower plate is arranged below the upper plate, and the upper plate and the lower plate are connected by two support plates.

7. The pipetting workbench according to claim 6, wherein, Notches are arranged at the front and rear parts of the upper plate.

8. The pipetting workbench according to claim 1, characterized in that, The main reagent fixing rack is in a "mouth" shape, and a main reagent fixing opening for placing the main reagent test tube is arranged at the upper part of the main reagent fixing rack.