Confluence body structure for small gene sequencer
By designing the confluent fluid structure on a small gene sequencer and integrating heating components and runner plates, the problem of reagent temperature difference impacting the chip is solved, achieving efficient and reliable improvement in reagent heating and sequencing quality.
Patent Information
- Application Number
- CN202422136126.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-02
- Publication Date
- 2025-07-25
- Estimated Expiration
- 2034-09-02
AI Technical Summary
Due to size limitations, small gene sequencers cannot install heating platforms on a large scale, resulting in the temperature difference of sequencing reagents that impact the chip, affecting life and sequencing quality.
A bus fluid structure is designed, including a pressure plate, a heating assembly and a runner plate, the runner plate is heated entirely through the heating assembly, the heating reliability is ensured using a shielding layer, the sequencing reagent runner and a cleaning runner are integrated, and the reagent flow is controlled using a solenoid valve.
It realizes efficient and reliable reagent heating on small gene sequencers, reduces the runner footprint and improves sequencing quality and efficiency.
Smart Images
Figure CN223150538U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of gene detection equipment, in particular to a fluid collector structure for a small gene sequencer. Background Art
[0002] In the process of gene sequencing, reagents flow from a reagent kit into the fluid channel of a sequencing chip through a pipeline for biochemical reactions. The temperature required for the biochemical reactions is controlled by a temperature control device. When the reagent flows from the pipeline into the fluid channel, if the temperature difference between the reagent and the reaction system is large, the sequencing chip will be strongly impacted, which will affect the service life of the sequencing chip and the reliability of sequencing. If the temperature distribution in the reaction system within the fluid channel is uneven, it will also affect the sequencing quality. Therefore, the sequencing reagent needs to be preheated before entering the sequencing chip, so as to reduce the time required for the reagent to heat up in the chip. By preheating, the sequencing time can be shortened and the sequencing efficiency can be improved. However, for current small gene sequencers, due to the limitation of the size of the instrument itself, it is impossible to install a large-area heating platform in the liquid pipeline, which will affect the sequencing progress. Summary of the Utility Model
[0003] In view of the above problems, the utility model provides a fluid collector structure for a small gene sequencer. By converging the liquid flow channels together and heating them simultaneously, the floor area occupied by the liquid path can be effectively reduced, and the sequencing quality can be improved at the same time.
[0004] The technical solution of the utility model is as follows:
[0005] A fluid collector structure for a small gene sequencer, comprising a pressing plate, a heating component and a flow channel plate which are stacked in sequence;
[0006] The flow channel plate is internally provided with a microfluidic channel, and the microfluidic channel includes a main channel and a plurality of branch channels communicated with the main channel;
[0007] The heating component includes a shielding layer, a heating layer and a heat conducting layer which are stacked in sequence, and the shielding layer is close to the pressing plate.
[0008] The heating layer is a heating film.
[0009] The heat conducting layer is a copper sheet.
[0010] The number of the branch channels is not less than five, and an electromagnetic valve is arranged on each branch channel.
[0011] The main channel includes an inlet liquid channel and an outlet liquid channel, and the branch channels include a sequencing reagent channel, a cleaning channel and a reserved channel. One end of each branch channel is communicated with the inlet liquid channel, and the other end is communicated with the outlet liquid channel.
[0012] The flow channel plate includes a cover plate and a micro flow channel plate that are snap-fitted to each other. The cover plate is connected to the heating component, and a liquid inlet and a liquid outlet are provided at one end of the micro flow channel plate away from the cover plate.
[0013] A sealing gasket is provided between the pressing plate and the heating component.
[0014] The beneficial effects of the present utility model are as follows:
[0015] By providing a flow channel plate, flow channels such as a sequencing reagent flow channel and a cleaning flow channel are integrated in one flow channel plate. All flow channels are heated by a heating component, and the reliability of heating is ensured through a shielding layer. The pressing plate tightly fits the heating component and the flow channel plate together by pressing, which can ensure the reliability of reagent heating. Description of the Drawings
[0016] Figure 1 is a schematic diagram of the overall structure of a confluence structure for a small gene sequencer according to an embodiment of the present utility model;
[0017] Figure 2 is a front view of a confluence structure for a small gene sequencer according to an embodiment of the present utility model;
[0018] Figure 3 is an exploded view of a confluence structure for a small gene sequencer according to an embodiment of the present utility model;
[0019] Figure 4 is a front view of a flow channel plate for a small gene sequencer according to an embodiment of the present utility model;
[0020] Figure 5 is a bottom view of a flow channel plate for a small gene sequencer according to an embodiment of the present utility model.
[0021] Description of the Reference Numerals:
[0022] 1 is the pressing plate, 2 is the heating component, 3 is the flow channel plate, 4 is the solenoid valve, 5 is the liquid inlet, 6 is the liquid outlet, 7 is the sealing gasket, 21 is the shielding layer, 22 is the heating layer, 23 is the heat conducting layer, 31 is the cover plate, and 32 is the micro flow channel plate. Detailed Embodiments
[0023] The following further describes the embodiments of the present utility model with reference to the drawings. Embodiment
[0024] As Figures 1 - 5 shown, a confluence structure for a small gene sequencer includes a pressing plate 1, a heating component 2, and a flow channel plate 3 that are stacked in sequence;
[0025] The flow channel plate 3 is internally provided with microfluidic channels, and the microfluidic channels include a main channel and a plurality of branch channels communicating with the main channel;
[0026] The heating component 2 includes a shielding layer 21, a heating layer 22 and a heat conduction layer 23 stacked in sequence, and the shielding layer 21 is close to the pressing plate 1.
[0027] The working principle of the above technical solution is as follows:
[0028] The flow channel plate 3 is a cuboid plate structure. By designing complex microfluidic channels, a liquid inlet and a liquid outlet are arranged at the bottom, and a plurality of precise and complex channels are arranged inside, including a main channel and a plurality of branch channels communicating with the main channel. By integrating a plurality of microfluidic channels on one flow channel plate, the integration of multiple sequencing reagent channels can be realized, the floor area of the channels can be effectively reduced, the layout of the sequencing liquid path can be facilitated, the quantitative control of the sequencing reagent can be realized, and at the same time, the heating component 2 can be pressed against one side of the flow channel plate 3 to realize the overall heating of the flow channel plate 3, thereby ensuring that the reagent is uniformly heated in the flow channel plate 3.
[0029] The heating layer 22 is a heating film, which is attached to the flow channel plate 3, which can ensure that the flow channel plate 3 can be comprehensively heated and the reliability of reagent heating can be ensured.
[0030] The heat conduction layer 23 is a copper sheet. Copper has good heat conductivity, which can ensure that heat can be transferred to the flow channel plate 3 and further ensure the reliability of reagent heating.
[0031] The number of branch channels is not less than five, and an electromagnetic valve 4 is arranged on each branch channel. The main channel includes a liquid inlet channel and a liquid outlet channel. The branch channels include a sequencing reagent channel, a cleaning channel and a reserved channel. One end of each branch channel communicates with the liquid inlet channel, and the other end communicates with the liquid outlet channel, which can ensure that during the sequencing process, the A, T, G, C, Buffer, and waste liquid channels can enter the flow channel plate in sequence. By controlling the opening and closing of the electromagnetic valve 4, the channels can be alternately cleaned, ensuring that different reagents will not be affected by the remaining reagents when entering the chip, ensuring the reliability of sequencing and improving the sequencing quality.
[0032] The flow channel plate 3 includes a cover plate 31 and a microchannel plate 32 that are buckled with each other. The cover plate 31 is connected to the heating component 2, and a liquid inlet 5 and a liquid outlet 6 are arranged at one end of the microchannel plate 32 away from the cover plate 31, which can ensure that the flow channel plate 3 will not leak and ensure the safety of the reagent during the sequencing process.
[0033] A sealing gasket 7 is arranged between the pressing plate 1 and the heating component 2, which can ensure the reliability of the connection between the pressing plate 1 and the heating component 2.
[0034] The above-described embodiments merely represent the specific implementation manners of the present utility model. The description thereof is relatively specific and detailed, but it should not be construed as a limitation on the scope of the patent of the present utility model. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present utility model, several modifications and improvements can still be made, and these all fall within the protection scope of the present utility model.
Claims
1. A current collector structure for a small gene sequencer, characterized in that, It includes a pressing plate, a heating component, and a flow channel plate that are stacked in sequence; The flow channel plate is internally provided with a microfluidic flow channel, and the microfluidic flow channel includes a main flow channel and a plurality of branch flow channels communicating with the main flow channel; The heating component includes a shielding layer, a heating layer, and a heat conduction layer that are stacked in sequence, and the shielding layer is close to the pressing plate.
2. The current collector structure for a small gene sequencer according to claim 1, characterized in that, The heating layer is a heating film.
3. The confluence structure for a small gene sequencer according to claim 1, characterized in that, The heat conduction layer is a copper sheet.
4. The current collector structure for a small gene sequencer according to claim 1, characterized in that, The number of the branch flow channels is not less than five, and an electromagnetic valve is arranged on each branch flow channel.
5. A current collector structure for a small gene sequencer according to claim 1, characterized in that, The main flow channel includes a liquid inlet flow channel and a liquid outlet flow channel, and the branch flow channels include a sequencing reagent flow channel, a cleaning flow channel, and a reserved flow channel. One end of each branch flow channel communicates with the liquid inlet flow channel, and the other end communicates with the liquid outlet flow channel.
6. The confluence structure for a small gene sequencer according to claim 1, characterized in that, The flow channel plate includes a cover plate and a microchannel plate that are buckled with each other. The cover plate is connected to the heating component, and a liquid inlet and a liquid outlet are arranged at one end of the microchannel plate away from the cover plate.
7. The confluence structure for a small gene sequencer according to claim 1, characterized in that A sealing gasket is arranged between the pressing plate and the heating component.