Sample collection and preservation tube with sample filtering structure

Abstract

The utility model discloses a sample collection and preservation tube with a sample filtering structure, which comprises a bottle body, the upper surface of the bottle body is movably connected with a sealing device, the outer side surface of the bottle body is provided with a rectangular label groove, the inside of the bottle body is movably connected with a stirring device, a filtering device is arranged below the stirring device, and the filtering device is connected with the sealing device. The sealing device, the filtering device and the stirring device are all installed in the bottle body, the stirring device comprises a second groove, an anti-seepage ball, a stirring rod and a third sealing ring, the stirring device is buckled in the pipe cover, the stirring rod is fixedly connected to the bottom end of the stirring device, the second groove is formed in the outer surface of the stirring device, and the third sealing ring is arranged in the second groove. The anti-seepage ball is mounted in the second groove, and the anti-seepage ball in the cavity of the stirring rod can be replaced by a paraffin ball and a rubber ball. Through the structure, the anti-seepage ball is arranged in the stirring device, so that a channel in the middle of the stirring rod can be effectively blocked at normal temperature, and liquid in the sample collecting tube is prevented from leaking.

Description

Technical Field

[0001] The utility model relates to the technical field of sample collection and preservation tubes, and particularly relates to a sample collection and preservation tube with a sample filtering structure. Background Art

[0002] Human fecal samples are important sample sources for intestinal disease research and clinical detection. A part of human intestinal exfoliated cells enter the feces every day, and the nucleic acids of these exfoliated cells contain canceration signals. Because fecal nucleic acid detection has many advantages such as non-invasive examination and no need to restrict diet, it is often used for the primary screening and general survey of diseases such as colorectal cancer, and has more advantages than methods such as chemical or immunological fecal occult blood tests and total rectoscopy. Feces also contain a large number of intestinal microorganisms, and many of these bacteria are closely related to human diseases, such as Escherichia coli and Helicobacter pylori, and these bacteria will also be detected in feces.

[0003] In the traditional processes of collection, detection, and transportation, contamination is likely to occur during transportation and preservation, affecting the detection results. Patients may not be able to meet the requirement of multi-point sample collection during the collection process, which will also affect the detection results. When detecting, the tester needs to open the tube cap. When opening the preservation tube, the odor escapes, which affects the environment and causes discomfort to the tester. When using an instrument for detection, the tube cap needs to be opened in advance, which will affect the efficiency of nucleic acid extraction. Content of the Utility Model

[0004] The purpose of the utility model is to provide a sample collection and preservation tube with a sample filtering structure, a fecal sample collection and preservation tube. During detection, there is no need to open the preservation tube. The paraffin ball arranged in the cavity of the stirring rod can block the channel in the middle of the stirring rod at normal temperature to prevent the liquid in the sample collection tube from leaking. When placed on an instrument for heating, the paraffin ball can be melted to open the liquid injection channel. The paraffin ball in the cavity of the stirring rod can also be replaced with a rubber ball. When the instrument is running, the driving shaft rod is inserted into the cavity of the stirring rod in the sample collection tube to push out the rubber ball, and the liquid injection channel can also be opened. Without opening the cap, it can avoid being affected by the unpleasant odor, which is convenient and fast, thus improving the work efficiency. Just place the collection tube containing the sample in a fully automatic fecal nucleic acid extraction workstation, and "sample in, nucleic acid out" can be achieved.

[0005] To achieve the above purpose, a sample collection and preservation tube with a sample filtering structure is provided, including a bottle body. The upper surface of the bottle body is movably connected with a sealing device. A rectangular label slot is arranged on the outer surface of the bottle body. A stirring device is movably connected inside the bottle body. A filtering device is arranged below the stirring device. The sealing device, the filtering device, and the stirring device are all installed inside the bottle body.

[0006] Preferably, the sealing device includes a thread, a bottle mouth, a first groove, and a liquid injection port. The bottle mouth is opened at the upper end of the bottle body. The thread is provided on the outer surface of the bottle mouth. The inner surface of the tube cap in the sealing device is threaded and corresponds to the thread. The thread is movably connected to the tube cap. The first groove is provided on the outer surface of the tube cap. There are multiple first grooves on the outer surface of the tube cap. The liquid injection port is opened in the middle of the upper surface of the tube cap. The liquid injection port penetrates through the tube cap and is circular.

[0007] Preferably, the filtering device includes filter cotton, a spring, a pressing ring, a limiting ring, a one-way valve, a first sealing ring, a second sealing ring, and a limiting hole. The pressing ring is opened on the upper surface of the filter cotton. The spring is movably connected below the filter cotton. The limiting hole is in contact with the bottom of the filter cotton. The limiting block is arranged above the first sealing ring.

[0008] Preferably, the limiting ring is fixedly connected to the bottom end of the one-way valve. The first sealing ring and the second sealing ring are installed at the upper end of the one-way valve. The limiting hole is opened in the middle of the upper surface of the one-way valve.

[0009] Preferably, the stirring device includes a second groove, an anti-seepage ball, a stirring rod, and a third sealing ring. The stirring device is buckled inside the tube cap. The stirring rod is fixedly connected to the bottom end of the stirring device.

[0010] Preferably, the second groove is opened on the outer surface of the stirring device. The anti-seepage ball is installed inside the second groove.

[0011] Preferably, the anti-seepage ball is spherical. The third sealing ring is installed above the stirring device.

[0012] The beneficial effects of the present utility model: By setting the sealing device, the probability of sample contamination during collection, transportation, and storage can be effectively avoided. By setting the filtering device, the efficiency of nucleic acid extraction can be effectively improved. By setting the stirring device, the sample can be completely dispersed and suspended. The above structural settings can avoid opening the lid, avoid unpleasant odors, and are convenient and fast, which can effectively improve the work efficiency of testers.

[0013] Additional aspects and advantages of the present utility model will be given in part in the following description, become apparent in part from the following description, or be learned through the practice of the present utility model. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The present utility model will be further described below in conjunction with the drawings and embodiments;

[0015] Figure 1 It is a schematic diagram of the overall structure of a sample collection and preservation tube with a sample filtering structure of the present utility model;

[0016] Figure 2Schematic structural diagram of a sealing device for a sample collection and preservation tube with a sample filtering structure according to the present utility model;

[0017] Figure 3 Schematic structural diagram of a filtering device for a sample collection and preservation tube with a sample filtering structure according to the present utility model;

[0018] Figure 4 Schematic diagram of a stirring device for a sample collection and preservation tube with a sample filtering structure according to the present utility model.

[0019] Legend description:

[0020] 1. Bottle body; 2. Sealing device; 3. Filtering device; 4. Stirring device; 5. Label slot; 201. Thread; 202. Bottle mouth; 203. First groove; 204. Liquid injection port; 301. Filter cotton; 302. Spring; 303. Pressing ring; 304. Limiting ring; 305. Check valve; 306. First sealing ring; 307. Limiting hole; 308. Second sealing ring; 309. Limiting block; 401. Second groove; 402. Anti-seepage ball; 403. Stirring rod; 404. Third sealing ring. Specific implementation manners

[0021] This part will describe in detail the specific embodiments of the present utility model. The preferred embodiments of the present utility model are shown in the drawings. The role of the drawings is to supplement the description in the text part of the specification, enabling people to intuitively and vividly understand each technical feature and the overall technical solution of the present utility model, but it cannot be understood as a limitation on the protection scope of the present utility model.

[0022] Refer to Figures 1 to 4 , an embodiment of a sample collection and preservation tube with a sample filtering structure according to the present utility model includes a bottle body 1. A sealing device 2 is movably connected to the upper surface of the bottle body 1. A rectangular label slot 5 is provided on the outer surface of the bottle body 1. A stirring device 4 is movably connected inside the bottle body 1. A filtering device 3 is arranged below the stirring device 4. The sealing device 2, the filtering device 3, and the stirring device 4 are all to be installed inside the bottle body 1. The sealing device 2 includes a thread 201, a bottle mouth 202, a first groove 203, and a liquid injection port 204. The bottle mouth 202 is opened at the upper end of the bottle body 1. The thread 201 is provided on the outer surface of the bottle mouth 202. The inner surface of the tube cap in the sealing device 2 is thread-shaped and corresponds to the thread 201. The thread 201 is movably connected to the tube cap. The outer surface of the tube cap is provided with a groove to help increase the friction force and facilitate unscrewing the tube cap.

[0023] The first groove 203 is provided on the outer surface of the tube cover, and a plurality of first grooves 203 are provided on the outer surface of the tube cover. The liquid injection port 204 is provided in the middle of the upper surface of the tube cover. The liquid injection port 204 passes through the tube cover and is circular. The filter device 3 includes filter cotton 301, a spring 302, a pressure ring 303, a limiting ring 304, a one-way valve 305, a first sealing ring 306, a limiting hole 307, and a second sealing ring 308. The pressure ring 303 is provided on the upper surface of the filter cotton 301, and the limiting hole 307 contacts the bottom of the filter cotton 301. The limiting block 309 is provided above the first sealing ring 306. The spring 302 is made of stainless steel to prevent rust. The cylinder at the bottom of the filter cotton 301 is inserted into the limiting hole 307 to achieve a limiting effect.

[0024] The limiting ring 304 is fixedly connected to the bottom end of the one-way valve 305, the first sealing ring 306 and the second sealing ring 308 are installed at the upper end of the one-way valve 305, the limiting hole 307 is opened in the middle of the upper surface of the one-way valve 305, the stirring device 4 includes a second groove 401, an anti-seepage ball 402, a stirring rod 403, and a third sealing ring 404. The stirring device 4 is buckled inside the pipe cover, the stirring rod 403 is fixedly connected to the bottom end of the stirring device 4, the second groove 401 is opened at On the outer surface of the stirring device 4, an anti-seepage ball 402 is installed inside the second groove 401. The anti-seepage ball 402 in the cavity of the stirring rod 403 can be replaced with a paraffin ball and a rubber ball. The anti-seepage ball 402 is spherical. The third sealing ring 404 is installed above the stirring device 4. The anti-seepage ball 402 is a paraffin ball, which will melt when encountering high temperature. The anti-seepage ball 402 can also be replaced with a rubber ball. The groove on the stirring rod 403 passes through the tube cover, and tin foil is provided between the tube cover and the stirring rod 403.

[0025] Working Principle: First, check that the sample collection bottle is clean and uncontaminated. Open the tube cap, take a spoonful of feces, and squeeze it all into the preservation solution in the collection tube. Discard the spoon. Place the sample collection tube in the fully automated fecal nucleic acid extraction workstation (NucliART-BAC 2.0). When the anti-seepage ball 402 is used as a paraffin ball, it can block the channel in the middle of the stirring rod 403 at room temperature, preventing the liquid in the sample collection tube from leaking. When placed in the instrument, the instrument automatically heats up, and the high temperature can melt the paraffin ball and open the injection channel. The anti-seepage ball 402 can also be replaced with a rubber ball. When the instrument is running, the driving shaft is inserted into the cavity of the stirring rod 403 in the sample collection tube, pushing out the anti-seepage ball 402, and also opening the injection channel; after the channel is opened, the instrument injects the decontamination liquid into the sample collection tube from the injection channel, and the instrument drives the stirring rod 403 to rotate, so that the feces are completely dispersed and suspended. The collection module moves to the bottom of the sample collection tube and moves upward to support the one-way valve 305 at the bottom of the collection tube. The liquid enters the collection tube through the filter screen. The filtrate is used as the application liquid for the next step of nucleic acid purification, which can effectively improve the work efficiency of the detection personnel.

[0026] The embodiments of the present utility model have been described in detail above in conjunction with the accompanying drawings. However, the present utility model is not limited to the above embodiments, and various changes can be made without departing from the gist of the present utility model within the scope of knowledge possessed by those of ordinary skill in the art.

Claims

1. A sample collection and preservation tube with a sample filtering structure, including a bottle body (1), characterized in that, A sealing device (2) is movably connected to the upper surface of the bottle body (1). A rectangular label groove (5) is provided on the outer surface of the bottle body (1). A stirring device (4) is movably connected inside the bottle body (1). A filtering device (3) is arranged below the stirring device (4). The sealing device (2), the filtering device (3), and the stirring device (4) are all to be installed inside the bottle body (1).

2. The sample collection and preservation tube with a sample filtering structure according to claim 1, characterized in that, The sealing device (2) includes a thread (201), a bottle mouth (202), a first groove (203), and a liquid injection port (204). The bottle mouth (202) is opened at the upper end of the bottle body (1). The thread (201) is provided on the outer surface of the bottle mouth (202). The inner surface of the tube cap in the sealing device (2) is threaded and corresponds to the thread (201). The thread (201) is movably connected to the tube cap. The first groove (203) is provided on the outer surface of the tube cap. There are a plurality of first grooves (203) on the outer surface of the tube cap. The liquid injection port (204) is opened in the middle of the upper surface of the tube cap. The liquid injection port (204) penetrates through the tube cap and is circular.

3. The sample collection and preservation tube with a sample filtering structure according to claim 1, characterized in that, The filtering device (3) includes a filter cotton (301), a spring (302), a pressing ring (303), a limiting ring (304), a one-way valve (305), a first sealing ring (306), a second sealing ring (308), a limiting block (309), and a limiting hole (307). The pressing ring (303) is opened on the upper surface of the filter cotton (301). The spring (302) is movably connected below the filter cotton (301). The limiting hole (307) is in contact with the bottom of the filter cotton (301). The limiting block (309) is arranged above the first sealing ring (306).

4. The sample collection and preservation tube with a sample filtering structure according to claim 3, characterized in that, The limiting ring (304) is fixedly connected to the bottom end of the one-way valve (305). The first sealing ring (306) and the second sealing ring (308) are installed at the upper end of the one-way valve (305). The limiting hole (307) is opened in the middle of the upper surface of the one-way valve (305).

5. A sample collection and preservation tube with a sample filtering structure according to claim 1, characterized in that, The stirring device (4) includes a second groove (401), an anti-seepage ball (402), a stirring rod (403), and a third sealing ring (404). The stirring device (4) is buckled inside the tube cap. The stirring rod (403) is fixedly connected to the bottom end of the stirring device (4).

6. The sample collection and preservation tube with a sample filtering structure according to claim 5, characterized in that, The second groove (401) is opened on the outer surface of the stirring device (4). The anti-seepage ball (402) is installed inside the second groove (401).

7. The sample collection and preservation tube with a sample filtering structure according to claim 6, wherein, The anti-seepage ball (402) is spherical. The third sealing ring (404) is installed above the stirring device (4).

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