Biological sample storage device and biological sample storage system

Abstract

The utility model provides a biological sample storage device and a biological sample storage system. The biological sample storage device comprises a storage tank, a driving unit and a bearing unit for bearing a sample, the storage tank comprises a first part and a second part which can rotate relatively; the first part and the second part are covered to form a cavity for accommodating the bearing unit, and the bearing unit is connected with the first part; the second part is provided with an opening for a sample to pass through; the driving unit is located outside the cavity and is in transmission connection with the first part to drive the first part and the bearing unit to rotate relative to the second part. According to the technical scheme, the driving unit drives the first part and the bearing unit to rotate, and the second part does not rotate, so that the sample borne on the bearing unit corresponds to the opening of the second part for sampling; the driving unit is arranged outside the cavity and does not need to bear a low-temperature environment in the cavity, the driving unit is not prone to failure, and therefore the first part driven by the driving unit and the bearing unit can rotate smoothly.

Description

Technical Field

[0001] The present application relates to the technical field of biological sample storage, and in particular to a biological sample storage device and a biological sample storage system. Background Art

[0002] In existing biological sample storage systems, biological samples are stored in a tank containing a carrier unit for the biological samples. During the production process, the tank is welded together into a single structure, with only a single opening reserved for the sampling port. During sampling, the carrier unit inside the tank rotates to deliver the sample to the sampling port.

[0003] During the sampling process, existing biological sample storage systems require the tank to be fixed relative to the ground. A motion mechanism within the tank controls the movement of the carrier unit to deliver the sample to the sampling port. Because biological samples can be stored at temperatures as low as -80°C or even -196°C, the motion mechanism within the tank can easily fail in these low temperatures, rendering the carrier unit immobile. Utility Model Content

[0004] The purpose of the present application is to provide a biological sample storage device and a biological sample storage system to solve the problem of loss of biological samples due to failure of a motion mechanism at low temperatures.

[0005] The embodiment of the present application is implemented as follows:

[0006] In a first aspect, embodiments of the present application provide a biological sample storage device and a biological sample storage system, comprising:

[0007] A carrying unit for carrying a sample;

[0008] A storage tank; the storage tank comprises a first portion and a second portion that are capable of rotating relative to each other; the first portion and the second portion cover each other to form a cavity for accommodating the carrying unit, the carrying unit being connected to the first portion; the second portion is provided with an opening for passing the sample;

[0009] A driving unit is located outside the cavity and is in transmission connection with the first part to drive the first part and the carrying unit to rotate relative to the second part.

[0010] In the above technical solution, the driving unit is located outside the cavity, and the driving unit can drive the first part and the carrying unit to rotate relative to the second part. Therefore, during the sampling process, there may be no relative movement between the first part and the carrying unit. The carrying unit rotates driven by the first part to a position where the biological sample to be sampled corresponds to the opening of the second part, and then sampling is performed through the opening of the non-rotating second part. That is, the driving unit drives the first part and the carrying unit to rotate. The driving unit is outside the cavity and does not need to bear the low-temperature environment inside the cavity, and it is not easy to have the problem that sampling cannot be performed due to the inability of the carrying unit to rotate at low temperature, thereby causing loss of the biological sample.

[0011] Combined with the first aspect, in some alternative embodiments, the second part is disposed above the first part.

[0012] In the above technical solution, the second part is located above the first part, and the second part can maintain a state of covering the first part under the action of gravity, so that the first part and the second part maintain good sealing performance; in addition, since the cold quantity is generally located at the bottom, therefore, the second part is located above the first part, which can make the opening located in the upper region. When the sample passes through the opening, the loss of cold quantity can be reduced.

[0013] Combined with the first aspect, in some alternative embodiments, the first part is a tank body and the second part is a cover body.

[0014] In the above technical solution, the connection position between the first part and the second part is at a position relatively high on the storage tank, which can make the loss of cold quantity in the storage tank less.

[0015] Combined with the first aspect, in some alternative embodiments, a sealing ring is provided between the first part and the second part, and the second part can move in a direction away from or close to the first part.

[0016] In the above technical solution, the setting of the sealing ring can make the sealing effect between the first part and the second part better; at the same time, since the second part can move in a direction away from or close to the first part, on the one hand, it can adapt to the processing error of the first part through the movement of the second part during the rotation of the first part, and on the other hand, it can change the compression degree of the sealing ring, thereby reducing the frictional resistance from the sealing ring that the first part needs to overcome when rotating.

[0017] Combined with the first aspect, in some alternative embodiments, it further includes a first rack, the first part is installed on the first rack, the second part is slidably disposed on the first rack, and when the first part rotates, the second part is configured to be capable of moving adaptively in a direction away from or close to the first part.

[0018] In the above technical solution, during the rotation of the first part, the second part can move adaptively, so that the sealing ring can maintain good contact with the first part and the second part, thereby reducing the loss of cold quantity. At the same time, the adaptive movement of the second part adjusts the position between the first part and the second part to adjust the compression degree of the sealing ring, so that while taking into account the sealing effect, the rotation of the first part can be made smoother.

[0019] Combined with the first aspect, in some alternative embodiments, it further includes a first frame, and a driving member is arranged on the first frame, and the driving member is connected to the second part to drive the second part to move in a direction away from or close to the first part.

[0020] In the above technical solution, by setting the driving member to drive the second part to move in a direction away from the first part, the compression degree of the sealing ring by the first part and the second part can be changed. When the first part rotates, by moving the second part in a direction away from the first part, the resistance required to drive the rotation of the first part can be reduced.

[0021] Combined with the first aspect, in some alternative embodiments, the driving member is configured to be able to drive the second part to move between a first position and a second position along the rotation axis direction of the first part, and the second part is in contact with the sealing ring both at the first position and the second position.

[0022] In the above technical solution, when the second part is in the first position, the contact with the sealing ring is good, and a good sealing effect is achieved. During the rotation of the first part, by driving the driving member to move the second part from the first position to the second position, the deformation amount of the sealing ring located between the first part and the second part is reduced, and the acting force between the sealing ring and the first part and the second part is small. Therefore, the frictional force required during the rotation of the first part is small. At the same time, the second part still remains in contact with the sealing ring at the second position. Therefore, while reducing the rotation resistance of the first part, the sealing between the first part and the second part is still good.

[0023] Combined with the first aspect, in some alternative embodiments, it further includes a sensor for detecting the distance between the first part and the second part, the sensor is in signal connection with the driving member, and the driving member is configured to drive the second part to be in a state of compressing the sealing ring according to the signal of the sensor.

[0024] In the above technical solution, by setting the sensor to detect the distance between the first part and the second part, the distance between the first part and the second part can be better controlled, so as to more precisely take into account the sealing effect and the smooth rotation of the first part.

[0025] In combination with the first aspect, in some alternative embodiments, a guiding structure is further connected between the second part and the first frame. The guiding structure includes a guide post and a guide sleeve that are slidably engaged; the extending direction of the guide post is parallel to the rotation axis of the first part; one of the guide post and the guide sleeve is disposed on the first frame, and the other is disposed on the second part.

[0026] In the above technical solution, by providing a guiding structure between the first frame and the second part, the relative movement between the first frame and the second part can be made more stable.

[0027] In combination with the first aspect, in some alternative embodiments, an elastic member is connected between the second part and the first frame. The elastic member is disposed in a compressed state on the side of the second part facing the first part; or is disposed in a stretched state on the side of the second part away from the first part.

[0028] According to the above technical solution, by providing an elastic member between the second part and the first frame, part of the gravity of the second part can be transmitted to the first frame through the elastic member. Therefore, the acting force between the sealing ring and the first part or the second part can be reduced, and further, the frictional force brought by the sealing ring that needs to be overcome during the rotation of the first part can be reduced.

[0029] In combination with the first aspect, in some alternative embodiments, the elastic member is an elastic column; or, the elastic member is a spring, and the spring is sleeved on the guide post.

[0030] In combination with the first aspect, in some alternative embodiments, the sealing ring is a C-shaped sealing ring, and an installation ring is further included. The installation ring is located between the first part and the second part. Two sides of the C-shaped sealing ring are respectively compressed between the first part and the installation ring, and between the second part and the installation ring.

[0031] In the above technical solution, one side of the C-shaped sealing ring is compressed between the first part and the installation ring, and the other side is compressed between the second part and the installation ring, so that the C-shaped sealing ring is more stably disposed between the first part and the second part.

[0032] In combination with the first aspect, in some alternative embodiments, the top end face part of the first part is recessed downward, the sealing ring is disposed in the recessed area and protrudes above the top end face, and the second part compresses the sealing ring under the action of gravity and partially bears on the end face.

[0033] In the above technical solution, since the upper part of the sealing ring protrudes from the top end face of the first part, and the second part is borne on the top end face, that is, a part of the gravity of the second part directly acts on the first part, and the other part of the gravity compresses the sealing ring. Compared with the implementation mode in which the gravity of the second part all acts on the sealing ring, the deformation of the sealing ring in the above technical solution is small, and the resistance from the sealing ring that needs to be overcome when rotating the first part is also smaller.

[0034] Combined with the first aspect, in some alternative embodiments, the first part includes an inner shell, an outer shell and an upper end plate. An insulating chamber is formed between the inner shell and the outer shell. The surface of the upper end plate facing the second part is partially recessed downward to form an annular groove for arranging the sealing ring. The inner side of the upper end plate is welded to the inner shell, and the outer side is welded to the outer shell to seal the insulating chamber.

[0035] In the above technical solution, the upper end plate with an annular groove is used to accommodate the sealing ring, and the upper end plate is welded to the inner shell and the outer shell to obtain the first part, which can not only play the role of accommodating the sealing ring, but also reduce the deformation phenomenon that occurs after the inner shell and the outer shell are welded to connect the upper end plate.

[0036] Combined with the first aspect, in some alternative embodiments, the bottom of the bearing unit is fixed to the tank body.

[0037] In the above technical solution, the bottom of the bearing unit is fixed to the tank body, which can realize the movement of the bearing unit together with the tank body.

[0038] Combined with the first aspect, in some alternative embodiments, the bearing unit includes a bottom plate. The bottom plate is provided with a plurality of through holes penetrating the plate surface. Each through hole can be provided with a fixing member, and each fixing member is configured to independently adjust the distance between the bottom plate and the bottom wall of the tank body.

[0039] In the above technical solution, by adjusting the distance between the bottom plate and the bottom wall of the tank body through the fixing member, the inclination degree of the bearing unit relative to the rotation axis of the tank body can be changed, thereby adjusting the levelness of the bearing unit and the coaxiality with the tank body. On the one hand, it can enable the first part to rotate along a preset trajectory, and on the other hand, it enables a more accurate sampling operation of the biological sample on the bearing unit.

[0040] Combined with the first aspect, in some alternative embodiments, the through hole is provided with a thread, and the fixing member is a bolt that is threadedly engaged with the through hole. The tail of the bolt contacts the bottom wall of the tank body. Using a bolt as the fixing member has a simple structure, and the bolt is a standard part with low cost.

[0041] In combination with the first aspect, in some alternative embodiments, the carrying unit further includes a top plate, and a plurality of connecting members are connected between the bottom plate and the top plate. A basket is to be installed between the plurality of connecting members. One end of each connecting member is connected to the bottom plate, and the other end is connected to the top plate.

[0042] In the above technical solution, the structure of the carrying unit is simple. Correspondingly, it has a relatively light weight, making the weight of the biological sample storage device relatively light.

[0043] In combination with the first aspect, in some alternative embodiments, the connecting members are aluminum rod-shaped structural members.

[0044] Using aluminum rod-shaped structural members as the connecting members, on the one hand, it makes the connecting members have a relatively light weight, and on the other hand, the good thermal conductivity of the metal material can make the temperature distribution in the cavity more uniform.

[0045] In combination with the first aspect, in some alternative embodiments, the top plate is provided with a placement hole for the basket to pass through, and the edge of the placement hole is in concave-convex fit positioning with the top edge of the basket.

[0046] In the above technical solution, there is positioning between the top edge of the basket and the top plate, which can make the position of the basket on the carrying unit more accurate. Since the basket is the structure for carrying biological samples, therefore, more accurate sampling operations can be performed on the biological samples on the carrying unit.

[0047] In combination with the first aspect, in some alternative embodiments, a plurality of through holes are circumferentially provided in the area of the carrying unit close to the top plate. A fixing member can be provided in each through hole, and each fixing member is configured to independently adjust the distance between the carrying unit and the inner peripheral wall of the tank body.

[0048] In the above technical solution, by adjusting the distance between the top plate and the inner peripheral wall of the tank body with the fixing member, the inclination degree of the carrying unit relative to the rotation axis of the tank body can also be changed, thereby adjusting the levelness of the carrying unit and its coaxiality with the tank body. On the one hand, it can make the carrying unit rotate along a preset trajectory, and on the other hand, it enables more accurate sampling operations to be performed on the biological samples on the carrying unit.

[0049] In combination with the first aspect, in some alternative embodiments, the through holes are provided with threads, and the fixing members are bolts that are in threaded cooperation with the through holes, and the tails of the bolts are in contact with the inner peripheral wall of the tank body.

[0050] In combination with the first aspect, in some alternative embodiments, a bearing is connected between the bottom of the first part and the first frame. Among them, the inner ring of the bearing is fixedly connected to the first frame, and the outer ring of the bearing is connected to the first part.

[0051] In the above technical solution, a bearing is provided between the first part and the first frame, which enables the first part to rotate more smoothly.

[0052] In combination with the first aspect, in some alternative embodiments, a connecting ring is connected to one end of the first part away from the second part. The connecting ring is provided with a plurality of through holes extending in the direction of the rotation axis. Each through hole may be provided with a fixing member, and each fixing member is configured to independently adjust the distance between the connecting ring and the outer ring of the bearing.

[0053] In the above technical solution, the distance between the connecting ring and the outer ring of the bearing can be adjusted by fixing members at different positions, thereby adjusting the angle between the axis of the first part and the rotation axis of the first part, so that the first part rotates along a preset trajectory.

[0054] In combination with the first aspect, in some alternative embodiments, the through hole is provided with a thread, and the fixing member is a bolt that is threadedly engaged with the through hole. The tail of the bolt contacts the upper surface of the outer ring of the bearing.

[0055] In combination with the first aspect, in some alternative embodiments, the outer ring of the bearing is provided with teeth, and the drive unit includes a driving gear that meshes with the teeth of the outer ring.

[0056] In combination with the first aspect, in some alternative embodiments, a guide rail is installed on the first frame, a sliding seat is arranged on the guide rail, the driving gear is installed on the sliding seat, and an elastic member in a compressed state is arranged between the sliding seat and the first frame to apply a force towards the first part to the sliding seat.

[0057] In the above technical solution, a gear drive is adopted between the drive unit and the first part, and the driving gear is installed on the sliding seat. An elastic member in a compressed state is arranged between the sliding seat and the first frame, and the elastic member applies a force towards the first part to the sliding seat. Therefore, the driving gear and the toothed ring can be meshed more tightly under the action of the elastic member, making the movement accuracy of the first part higher and the sampling more accurate.

[0058] In combination with the first aspect, in some alternative embodiments, the elastic member is a spring or an elastic column.

[0059] In combination with the first aspect, in some alternative embodiments, the driving gear is a helical gear. Using a helical gear as the driving gear enables the first part to have high precision in both forward and reverse rotations, making it easy to control the rotation direction of the first part and saving sampling time.

[0060] In conjunction with the first aspect, in some optional embodiments, the transmission ratio between the driving gear and the outer ring is 3 to 10. With the transmission ratio of 3 to 10, the driving unit can drive the first part to rotate with less force.

[0061] In conjunction with the first aspect, in some optional embodiments, the drive unit is located on a side of the first portion away from the second portion, and a projection of the drive unit is located within a contour of a projection of the first portion in the direction of the rotational axis of the first portion. By arranging the drive unit within the contour of the projection of the first portion, the space occupied by the biological sample storage device can be reduced.

[0062] In combination with the first aspect, in some optional embodiments, the cover body is a plate-shaped structural member, and a thermal insulation layer is connected to a side of the cover body facing the tank body.

[0063] In the above embodiment, the cover is a plate-shaped structural member, which can reduce the size of the biological sample storage device in the direction of the rotation axis of the first part. The heat insulation layer provided on the cover can reduce the loss of cold in the cavity.

[0064] In combination with the first aspect, in some optional embodiments, a liquid nitrogen delivery pipe is further provided in the thermal insulation layer, and the liquid nitrogen delivery pipe is provided with a liquid outlet facing the cavity.

[0065] In the above embodiment, liquid nitrogen can be added into the cavity through the liquid nitrogen delivery pipe, and the liquid nitrogen delivery pipe passes through the insulation layer, which can reduce the cooling loss of the liquid nitrogen.

[0066] In combination with the first aspect, in some optional embodiments, the liquid nitrogen delivery pipe is further connected to a liquid nitrogen supply device; and the liquid outlet is an atomization port.

[0067] In the above technical solution, liquid nitrogen is used for refrigeration, which can meet the refrigeration condition of -196°C; when the liquid outlet is an atomizing port, the refrigeration condition of -80°C can be met.

[0068] In combination with the first aspect, in some optional embodiments, the liquid nitrogen delivery pipe includes an arcuate segment and a straight segment connected to the arcuate segment; and both the arcuate segment and the straight segment are provided with the liquid outlet.

[0069] In the above technical solution, the liquid nitrogen delivery pipe includes an arc segment and a straight segment, and both the arc segment and the straight segment are provided with liquid outlets, so that liquid nitrogen can flow into the cavity from more positions above the cavity, so that the temperature distribution in the cavity is more uniform.

[0070] In combination with the first aspect, in some optional embodiments, the carrying unit includes a bottom plate, and a gap for accommodating liquid nitrogen is provided between the bottom plate and the bottom wall of the tank body; the biological sample storage device also includes a sensor assembly, the sensor assembly includes a liquid level sensor, and the detection end of the liquid level sensor is located in the gap.

[0071] In the above technical solution, liquid nitrogen can be used for refrigeration, and the amount of liquid nitrogen can be monitored by a liquid level sensor.

[0072] In combination with the first aspect, in some optional embodiments, a detection channel with two ends extending therethrough is provided at the rotation axis of the carrying unit; the sensor assembly includes a mounting rod, the mounting rod passes through the detection channel and extends into the gap, and the liquid level sensor is provided at the end of the mounting rod.

[0073] In the above technical solution, by setting a detection channel at the rotation axis of the carrying unit and passing the mounting rod through the detection channel, the liquid level detection of liquid nitrogen can be achieved, and during the rotation of the first part and the carrying unit, the mounting rod is not likely to interfere with the carrying unit.

[0074] In combination with the first aspect, in some optional embodiments, the sensor assembly further includes a connecting disk and a cylindrical structure that are interconnected, the connecting disk is connected to the cover body, the cylindrical structure is located in the insulation layer, and the mounting rod is connected to one end of the cylindrical structure away from the connecting disk and extends in a direction away from the insulation layer; the sensor assembly further includes a temperature sensor, and the temperature sensor and the liquid level sensor are both arranged on the mounting rod.

[0075] In the above technical solution, the sensor assembly is connected to the cover body via the connecting plate. During the sampling process, the cover body does not move, so there will be no problem of the sensor signal lines being entangled with each other.

[0076] In combination with the first aspect, in some optional embodiments, the connecting disk has a first opening, the tubular structure has a second opening, the first opening is aligned with the second opening, and the signal lines of the liquid level sensor and the temperature sensor pass through the second opening of the tubular structure and the first opening.

[0077] In the above technical solution, the second opening of the tubular structure is aligned with the first opening of the connection disk, which can facilitate the passage of the sensor signal line and the placement of insulation material in the tubular structure.

[0078] In combination with the first aspect, in some optional embodiments, one end of the mounting rod is a threaded segment, the threaded segment is located in the cylindrical structure, a nut is provided in the cylindrical structure and is connected to the threaded segment to connect the mounting rod to the cylindrical structure.

[0079] In the above technical solution, the nut connecting the mounting rod and the cylindrical structural member is arranged inside the cylindrical structural member, which will not cause an increase in the size of the biological sample storage device in the direction of the rotation axis of the first part.

[0080] Combined with the first aspect, in some alternative embodiments, the carrying unit has a plurality of placement positions distributed along the circumference, and the biological samples are arranged at the placement positions; during the rotation of the storage tank, the placement positions pass below the opening.

[0081] In the above technical solution, when the placement position is below the opening, the biological sample arranged at the placement position can be sampled.

[0082] Combined with the first aspect, in some alternative embodiments, in the projection along the rotation direction of the storage tank, the placement positions are arranged in at least two circles; the number of the openings is one, and the opening extends along the radial direction of the second part, so that after the first part rotates, the biological samples at different placement positions can be taken out from the opening.

[0083] In the above technical solution, even if the placement positions are arranged in at least two circles, the number of the openings for sampling in the second part is only one, so that the structure for opening the sampling cover covering the opening is relatively simple.

[0084] In the second aspect, an embodiment of the present application provides a biological sample storage system, including an access unit and the biological sample storage device provided in the first aspect, where the access unit is located on a side of the second part away from the first part; the access unit can move to the opening to access samples from the opening.

[0085] When using the biological sample storage system provided by the above technical solution, the driving unit drives the first part to drive the carrying unit to rotate together, and it is not necessary for the carrying unit to rotate relative to the first part in the low-temperature cavity. Therefore, the problem that the carrying unit fails to move in the cavity can be effectively solved.

[0086] Combined with the second aspect, in some alternative embodiments, it further includes a second rack, and the access unit is installed on the second rack; the second rack is detachably connected to the storage tank, and the second rack is connected with an adjustable support frame, and the adjustable support frame is configured to separate the second rack from the storage tank.

[0087] In the above technical solution, the second rack can be separated from the first rack through the adjustable support frame, and then the first rack together with the storage tank can be taken out, so that manual sampling of the samples in the storage tank can be realized. Description of the Drawings

[0088] To more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings required for the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation of the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings.

[0089] Figure 1 Partial structural schematic diagram of the biological sample storage system provided by the embodiment of the present application;

[0090] Figure 2 Partial structural schematic diagram of the biological sample storage device provided by the embodiment of the present application;

[0091] Figure 3 is Figure 2 The enlarged view of part A in;

[0092] Figure 4 Schematic diagram of the driving unit arranged on the top of the storage tank in the embodiment of the present application;

[0093] Figure 5 Schematic diagram of the driving unit arranged in the storage tank in the embodiment of the present application;

[0094] Figure 6 Schematic diagram of the driving unit being a hollow rotating platform in the embodiment of the present application;

[0095] Figure 7 Schematic diagram of the rotation axis of the first part in the horizontal direction in the embodiment of the present application;

[0096] Figure 8 One of the installation schematic diagrams of the sealing ring in the embodiment of the present application;

[0097] Figure 9 Another installation schematic diagram of the sealing ring in the embodiment of the present application;

[0098] Figure 10 Top view of the biological sample storage device in the embodiment of the present application;

[0099] Figure 11 is Figure 10 The enlarged view of part B in;

[0100] Figure 12 Schematic diagram of a spring arranged outside the guide post;

[0101] Figure 13 Schematic diagram of the driving member connected to the second part;

[0102] Figure 14 Schematic diagram of the bearing unit arranged in the cavity in the embodiment of the present application;

[0103] Figure 15 is Figure 14 a sectional view of the structure shown;

[0104] Figure 16 is Figure 15 a partial schematic view at position C in;

[0105] Figure 17 is Figure 15 a partial schematic view at position D in;

[0106] Figure 18 is a schematic view of the liquid nitrogen delivery pipe within the insulation layer;

[0107] Figure 19 is a schematic view of the second part connected to the insulation layer;

[0108] Figure 20 is a schematic view of the liquid nitrogen delivery pipe;

[0109] Figure 21 is a schematic view of the first part in the embodiment of the present application;

[0110] Figure 22 is a schematic view of the drive unit installed on the first frame;

[0111] Figure 23 is a schematic view of the sensor assembly;

[0112] Figure 24 is Figure 23 a schematic view of the sensor cutaway mounting disc and the cylindrical structural member in;

[0113] Figure 25 is a schematic view of the sensor assembly connected to the second part;

[0114] Figure 26 is one of the schematic views where both the first part and the second part are tanks;

[0115] Figure 27 is another schematic view where both the first part and the second part are tanks;

[0116] Figure 28 is a schematic view of the sealing ring and the first part jointly bearing the second part;

[0117] Figure 29 is a schematic view of the first frame and the second frame in the connected state;

[0118] Figure 30 is a schematic view after removing the first frame and the storage tank;

[0119] Figure 31 is one of the schematic views of driving the first part to rotate by a motor;

[0120] Figure 32 The second schematic diagram of the first part driven by a motor to rotate.

[0121] Icons: 010 - Biological sample storage device; 100 - Storage tank; 110 - First part; 111 - Inner shell; 112 - Outer shell; 113 - Upper end plate; 1131 - Annular groove; 120 - Second part; 121 - Opening; 200 - Carrying unit; 210 - Top plate; 211 - Placing hole; 2111 - Groove; 220 - Bottom plate; 221 - First through hole; 230 - Connecting piece; 240 - Detection channel; 250 - Fixed block; 251 - Second through hole; 300 - Driving unit; 310 - Driving gear; 320 - Ring gear; 330 - Motor; 341 - Worm gear; 342 - Worm; 350 - Sliding seat; 360 - Guide rail; 400 - Sealing ring; 410 - First flange; 420 - Second flange; 500 - First frame; 510 - Chassis; 520 - Column; 600 - Driving part; 710 - Guide post; 720 - Guide sleeve; 800 - Elastic part; 900 - Mounting ring; 1010 - Outer ring; 1100 - Connecting ring; 1110 - Third through hole; 1120 - Pressing block; 1200 - Thermal insulation layer; 1300 - Liquid nitrogen delivery pipe; 1310 - Arc section; 1320 - Straight section; 1400 - Sensor assembly; 1410 - Liquid level sensor; 1420 - Temperature sensor; 1430 - Connecting plate; 1440 - Cylindrical structural member; 1450 - Mounting rod; 1460 - Signal line; 1500 - Basket; 1520 - Rod-shaped structural member; 1600 - Sampling cover; 020 - Access unit; 0210 - Cover opening assembly; 0220 - Cold trap; 030 - Second frame; 040 - Adjustable support frame. Specific embodiments

[0122] To make the objectives, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. Apparently, the described embodiments are some, but not all, of the embodiments of the present application. Usually, the components of the embodiments of the present application described and illustrated herein can be arranged and designed in various different configurations.

[0123] Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the present application claimed, but merely represents selected embodiments of the present application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present application without creative efforts shall fall within the scope of protection of the present application.

[0124] It should be noted that similar reference numerals and letters indicate similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0125] In the description of the present application, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of this application is usually placed during use. It is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present application. In addition, the terms "first", "second", "third", etc. are only used for distinguishing descriptions and cannot be understood as indicating or implying relative importance.

[0126] In addition, the terms "horizontal", "vertical", "hanging", etc. do not mean that the components are required to be absolutely horizontal or hanging, but can be slightly inclined. For example, "horizontal" only means that its direction is more horizontal relative to "vertical", and does not mean that the structure must be completely horizontal, but can be slightly inclined.

[0127] In the description of the present application, it should also be noted that unless otherwise clearly specified and limited, the terms "set", "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected, or indirectly connected through an intermediate medium, and can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific situations.

[0128] The inventors of the present application found that in existing biological sample storage devices, the storage tank is an integral structure obtained by welding and is non-detachable; in order to reduce heat dissipation, the size of the sampling port for sampling on the storage tank is also small. In order to sample through the small-sized sampling port, a motion unit is usually arranged inside the storage tank, and the motion unit controls the carrier unit to rotate inside the storage tank so that the sample on the carrier unit moves to the sampling port for sampling through the sampling port. However, since the motion mechanism is located inside the storage tank and the temperature inside the storage tank is as low as -80°C or even -1'96°C, the motion mechanism is prone to failure, causing the carrier unit to have a motion failure due to low temperature, resulting in the loss of biological samples. And the storage environment of biological samples makes it necessary for the carrier unit to be in a low-temperature environment. Based on this, the inventors of the present application provide a biological sample storage system and a biological sample storage device, which drive the unit to be externally placed in the cavity and control the rotation of the first part and the carrier unit, so as to avoid the problem of motion failure of the drive unit and the carrier unit at low temperature.

[0129] As shown in the embodiments of the biological sample storage device 010 provided by the present application Figures 1 to 25 as follows, please refer to Figure 1 , Figure 2 , Figure 6 and Figure 14 . The biological sample storage device 010 includes a carrying unit 200, a storage tank 100 and a driving unit 300. Among them, the carrying unit 200 is used to carry samples; the storage tank 100 includes a first part 110 and a second part 120, and the first part 110 and the second part 120 are covered to form a cavity for accommodating the carrying unit 200; the carrying unit 200 is connected to the first part 110, and the driving unit 300 is arranged outside the cavity and connected to the first part 110 to drive the first part 110 to rotate relative to the second part 120. Optionally, the first part 110 and the carrying unit 200 are configured such that when the first part 110 rotates relative to the second part 120, the first part 110 drives the carrying unit 200 to rotate relative to the second part 120 together.

[0130] As Figure 2 shown, the second part 120 is provided with an opening 121 for the sample to pass through. It is not difficult to understand that the opening 121 of the second part 120 can be used as a sampling port for sampling. Please refer to Figure 10 , Figure 14 and Figure 15 . By rotating the carrying unit 200 relative to the second part 120, the samples at different positions on the carrying unit 200 can be rotated to the positions corresponding to the opening 121, so as to realize sampling of the samples at different positions (that is, when sampling, the first part 110 rotates and the second part 120 does not rotate, so that the samples on the carrying unit 200 that rotate synchronously with the first part 110 rotate to the opening 121 of the second part 120). In order to avoid the loss of cold in the cavity, as Figure 25 shown, the opening 121 of the second part 120 is covered with a sampling cover 1600. It should not be difficult for those skilled in the art to understand that the size of the opening 121 of the second part 120 should be as small as possible under the condition of meeting the requirement of smooth sampling to reduce the loss of cold during sampling.

[0131] In the biological sample storage device 010 provided by the present application, the carrying unit 200 is connected to the first part 110 and rotates relative to the second part 120 under the drive of the first part 110. Therefore, during the sampling process, the carrying unit 200 and the first part 110 rotate together rather than rotate independently. Therefore, the carrying unit 200 can have no relative movement with the first part 110, thus solving the problem of loss of biological samples caused by the failure of the movement of the carrying unit 200 at low temperature.

[0132] Further, in some embodiments, the second part 120 is disposed above the first part 110. For example, the second part 120 is directly above the first part 110, that is, the direction of the gravity acting on the second part 120 is parallel to the axis about which the first part 110 rotates relative to the second part 120 (that is, the axis direction of the rotation of the first part 110 is the vertical direction). In some other embodiments, the second part 120 may also be obliquely above the first part 110. Correspondingly, the axis about which the first part 110 rotates relative to the second part 120 has a certain angle with the vertical direction. Even in some embodiments, as Figure 7 shown, the axis about which the first part 110 rotates relative to the second part 120 may also be horizontal.

[0133] In the embodiments where the second part 120 is disposed above the first part 110, correspondingly, the second part 120 can be kept in a state of covering the first part 110 under the action of gravity, so that a good sealing property can be maintained between the first part 110 and the second part 120; in addition, since the cold quantity is generally located at the bottom, therefore, when the second part 120 is located above the first part 110, the opening 121 can be located in the upper region, and when the sample passes through the opening 121, the loss of cold quantity can be reduced.

[0134] In some embodiments, the first part 110 is a tank body and the second part 120 is a cover body. As Figure 14 、 Figure 15 and Figure 21 shown, the first part 110 being a tank body means that the first part 110 includes a bottom wall and a side wall surrounding in a ring shape along the edge of the bottom wall, and the bottom wall and the side wall enclose a cylindrical structure. The second part 120 being a cover body means that the size of the second part 120 is adapted to the size of the top of the tank body, and can cover the top of the tank body to form a cavity, and moreover, the size of the second part 120 in the direction of the rotation axis of the first part 110 is smaller. As in the embodiments shown in Figure 2 and Figure 25 , the cover body is a flat structural member, and the size of the cover body in the direction of the rotation axis of the first part 110 is the thickness of the cover body. In some other embodiments where the second part 120 is a cover body, the second part 120 may also be a structural member with a certain arc similar to the shape of a pot lid, that is, the second part 120 includes a plate-like structure with a curved surface.

[0135] When the storage tank 100 stores biological samples, its interior is in a low-temperature state, and there is a temperature stratification phenomenon inside the storage tank 100, that is, the temperature at the top of the storage tank 100 is higher and the temperature at the bottom is lower. In the embodiment where the first part 110 is the tank body and the second part 120 is the cover body, the connection position between the first part 110 and the second part 120 is at a higher position of the storage tank 100, which can reduce the loss of cold in the storage tank 100, that is, reduce the loss of cold air in the cavity.

[0136] Furthermore, in the embodiment where the second part 120 is a flat plate structure, the size of the storage tank 100 in the direction of the rotation axis of the first part 110 can be reduced to a certain extent, that is, the Figure 2 height of the storage tank 100 in can be reduced, so as to facilitate the transfer of the storage tank 100 into the elevator for transportation.

[0137] In some other embodiments, it may also be that the first part 110 is a plate-like structural member and the second part 120 is a tank-like structural member; it may also be Figure 26 as shown, both the first part 110 and the second part 120 are tank-like structural members and the dimensions of the first part 110 and the second part 120 in the direction of the rotation axis of the first part 110 are similar; it may also be Figure 27 as shown, both the first part 110 and the second part 120 are tank-like structural members, and in the direction of the rotation axis of the first part 110, the dimension of the first part 110 is smaller than that of the second part 120. In the embodiment where the second part 120 is located above the first part 110, when the first part 110 is the tank body and the second part 120 is the cover body, the weight of the second part 120 can be made smaller, so that the force exerted by the second part 120 on the first part 110 is smaller, and thus the first part 110 can be rotated more easily.

[0138] Since the storage tank 100 provided in the present application includes the first part 110 and the second part 120, and the first part 110 can rotate relative to the second part 120, there is a gap at the connection position between the first part 110 and the second part 120, as Figure 8 and Figure 9 shown. In order to reduce the cold loss from this gap position, a sealing ring 400 can also be provided between the first part 110 and the second part 120. In some embodiments, the sealing ring 400 may be connected to the first part 110, and in some other embodiments, the sealing ring 400 may be connected to the second part 120. Furthermore, in the embodiments where the second part 120 is directly above, obliquely above the first part 110, and the rotation axis of the first part 110 relative to the second part 120 is horizontal, a sealing ring 400 can be provided between the first part 110 and the second part 120.

[0139] In the embodiment shown as follows Figure 9 in order to facilitate the installation of the sealing ring 400, an installation ring 900 is further provided between the first part 110 and the second part 120, and the sealing ring 400 adopted is a C-shaped sealing ring. The installation ring 900 is a planar ring-shaped structural member with opposite first and second planes, where the first plane faces the first part 110 and the second plane faces the second part 120. One side of the C-shaped sealing ring has a first flange 410 and the other side has a second flange 420. The two sides of the C-shaped sealing ring are respectively compressed between the first part 110 and the installation ring 900, and between the second part 120 and the installation ring 900, that is, the first flange 410 is compressed between the first plane of the installation ring 900 and the first part 110, and the second part 120 is compressed between the second plane of the installation ring 900 and the second part 120.

[0140] Furthermore, in the embodiment where the sealing ring 400 is connected to the first part 110, the installation ring 900 is connected to the first part 110, and the installation ring 900 and the first part 110 are connected by bolts, and a gap for accommodating the first flange 410 of the C-shaped sealing ring is reserved between the first surface of the installation ring 900 and the first part 110. Similarly, in the embodiment where the sealing ring 400 is connected to the second part 120, the installation ring 900 is connected to the second part 120, and a gap for accommodating the second flange 420 of the C-shaped sealing ring is reserved between the second surface of the installation ring 900 and the second part 120.

[0141] In some embodiments, as shown Figures 1 to 3 in the figure, the biological sample storage device 010 further includes a first frame 500. The first part 110 is installed on the first frame 500, and the second part 120 is slidably arranged on the first frame 500 and is located above the first part 110. Among them, the first frame 500 is a structure that remains stationary relative to the ground during the sampling process. When the first part 110 rotates, the second part 120 moves adaptively in a direction closer to or farther from the first part 110. It is not difficult to understand that due to installation errors and processing errors, etc., the flatness of the top surface of the first part 110 may not be high, and there may be a certain angle between the top surface of the first part 110 and the horizontal plane. Therefore, during the rotation of the first part 110, the second part 120 can move adaptively, that is, it automatically moves in a direction away from the first part 110 under the action of the first part 110 and automatically moves in a direction closer to the first part 110 under the action of gravity, so that the sealing ring 400 can maintain good contact with the first part 110 and the second part 120 to reduce cold loss.

[0142] Most of the existing sealing rings 400 are of flexible structures to better fit with other structural components at the sealing position, thereby reducing possible gaps at the sealing position and ensuring the sealing effect. Correspondingly, materials of the existing sealing rings 400 mostly adopt silicone rubber, etc. Therefore, after the sealing ring 400 is arranged between the first part 110 and the second part 120, the sealing ring 400 contacts with the first part 110 and the second part 120. In this state, when the first part 110 rotates, the friction force existing between the first part 110 and the sealing ring 400 needs to be overcome, increasing the difficulty of rotating the first part 110. Especially when the second part 120 is arranged above the first part 110, the first part 110 needs to overcome a large friction force when rotating.

[0143] Therefore, in some embodiments, when the first part 110 and the second part 120 rotate relative to each other, the second part 120 can move in a direction away from or close to the first part 110, which can reduce the friction force between the first part 110 and the sealing ring 400 during the rotation of the first part 110. It is not difficult to understand that after the first part 110 and the second part 120 move away from each other, the deformation amount of the sealing ring 400 located between the first part 110 and the second part 120 decreases, and the acting force between the sealing ring 400 and the first part 110 and the second part 120 is small. Therefore, the friction force that needs to be overcome during the rotation of the first part 110 is small. Further, as Figure 13 shown, a driving member 600 can be arranged to drive the second part 120 and the first part 110 to move away from each other, so that during the process of the driving unit 300 driving the first part 110 to rotate for sampling, the driving member 600 drives the second part 120 and the first part 110 to move away from each other, thereby reducing the resistance from the sealing ring 400 that needs to be overcome during the rotation of the first part 110.

[0144] In the embodiment of using the driving member 600 to make the first part 110 and the second part 120 move away from each other, the driving member 600 can be connected to the second part 120, and the position of the second part 120 is changed by the driving member 600 to make it move away from the first part 110. As Figure 1 、 Figure 2 and Figure 13In the illustrated embodiment, the biological sample storage device 010 includes a first frame 500, a first portion 110 is mounted on the first frame 500, and a driving member 600 is mounted on the first frame 500 and connected to the second portion 120 to drive the second portion 120 to slide away from or close to the first portion 110. The driving member 600 may be an existing product such as a cylinder, a hydraulic cylinder, or an electric push rod that outputs linear motion; the driving member 600 may also be a lead screw slider structure. Correspondingly, the second portion 120 is connected to the slider. In some other embodiments, it may also be arranged that the driving member 600 drives the first portion 110 to move away from the second portion 120.

[0145] In the embodiment where the driving member 600 drives the second portion 120 away from the first portion 110, the moving positions of the second portion 120 include a first position close to the first portion 110 and a second position away from the first portion 110; the driving member 600 is configured to be able to drive the second portion 120 to move between the first position and the second position along the rotation axis direction of the first portion 110, and the second portion 120 is in contact with the sealing ring 400 at both the first position and the second position. Among them, the contact form between the second portion 120 and the sealing ring 400 at the first position includes surface contact and line contact, and the contact form between the second portion 120 and the sealing ring 400 at the second position includes surface contact, line contact, and point contact. It is not difficult to understand that when the second portion 120 is in the first position, the contact with the sealing ring 400 is good, and a good sealing effect is achieved. During the rotation of the first portion 110, the driving member 600 causes the second portion 120 to move from the first position to the second position, and the deformation amount of the sealing ring 400 located between the first portion 110 and the second portion 120 decreases, and the acting force between the sealing ring 400 and the first portion 110 and the second portion 120 is small. Therefore, the frictional force that needs to be overcome during the rotation of the first portion 110 is small. At the same time, the second portion 120 still remains in contact with the sealing ring 400 at the second position. Therefore, while reducing the rotation resistance of the first portion 110, the sealing between the first portion 110 and the second portion 120 is still good. Further, the connection between the driving member 600 and the second portion 120 can be a rigid connection, a flexible connection, or just a contact connection. In the case where the driving member 600 and the second portion 120 are rigidly connected, the second portion 120 moves between the first position and the second position together with the driving member 600; in the case where the driving member 600 and the second portion 120 are flexibly connected or just in contact connection, it may be that the driving member 600 pushes the second portion 120 from the first position to the second position, and then the second portion 120 returns to the first position under the action of gravity.

[0146] Further, in order to control the distance that the driving member 600 drives the second part 120 to move, in some embodiments of the present application, a sensor for detecting the distance between the first part 110 and the second part 120 is further provided. This distance can be the distance between any position in the first part 110 and any position in the second part 120. For example, in the direction of the rotation axis of the first part 110, it is the minimum distance between the first part 110 and the second part 120. The sensor is signal-connected to the driving member 600. The driving member 600 drives the second part 120 to be in a state of compressing the sealing ring 400 according to the signal of the sensor. That is, when the driving member 600 drives the second part 120 to move away from the first part 110, through the data detected by the sensor, the sealing ring 400 is always in a compressed state between the first part 110 and the second part 120, avoiding the loss of cold in the cavity due to the gap between the sealing ring 400 and the first part 110 or the second part 120. By providing a distance sensor, it is also possible to stop the movement when the second part 120 moves towards the first part 110 to the first position, or to stop the movement when the second part 120 moves away from the first part 110 to the second position.

[0147] For example, in one embodiment, the biological sample storage device 010 includes a control system, a sensor and a driving member 600 that are both signal-connected to the control system. The sensor can detect the distance between the first part 110 and the second part 120 and feed the data back to the control system. Preset data is stored in the control system. The preset data is: the maximum value of the distance between the first part 110 and the second part 120 when there is no gap between the sealing ring 400 and the first part 110 and the second part 120. The biological sample storage device 010 further includes a first rack 500. The first part 110 and the driving member 600 are both installed on the first rack 500. The output end of the driving member 600 is connected to the second part 120. When the first tank rotates, the control system sends a signal to the driving member 600 to make the second part 120 move away from the first part 110, and the sensor continuously detects the distance between the first part 110 and the second part 120. When the data fed back by the sensor to the control system is equal to the preset data, the control system sends a signal to the driving member 600 to make the second part 120 stop moving.

[0148] Of course, it is also possible not to set a sensor to detect the distance between the first part 110 and the second part 120. When the first part 110 rotates, the driving member 600 directly drives the second part 120 to move away from the first part 110 to the extreme position. When the second part 120 moves to the extreme position, if there is a gap between the sealing ring 400 and the first part 110 or the second part 120, although it will cause the leakage of cold air in the cavity, it can also reduce the frictional force required to overcome during the rotation of the first part 110. The appearance of a gap between the sealing ring 400 and the first part 110 or the second part 120 is not unacceptable. Moreover, when the second part 120 moves to the extreme position, the size of the gap between the sealing ring 400 and the first part 110 or the second part 120 can be controlled by the stroke of the driving member 600, etc., to avoid a large amount of loss of cold in the cavity.

[0149] In some other embodiments, before and after the first part 110 rotates, the distance between the second part 120 and the first part 110 may not change. By reducing the acting force between the sealing ring 400 and the first part 110 or the second part 120, the frictional force brought by the sealing ring 400 that needs to be overcome during the rotation of the first part 110 is reduced. That is, when setting the sealing ring 400, the sealing ring 400 is set between the first part 110 and the second part 120 in a state with a relatively small compression deformation amount.

[0150] One of the embodiments of setting the sealing ring 400 between the first part 110 and the second part 120 in a state with a relatively small compression deformation amount is as Figure 28As shown, there is mutual contact between the first part 110 and the second part 120. In this case, part of the gravity of the second part 120 is transmitted to the first part 110 by directly contacting the first part 110, and another part of the gravity of the second part 120 is transmitted to the first part 110 through the deformed sealing ring 400. Specifically, the top end face part of the first part 110 may be recessed downward, and the sealing ring 400 is arranged in the recessed area. Moreover, the upper part of the sealing ring 400 protrudes from the end face of the top of the first part 110. Under the action of gravity, the second part 120 compresses the sealing ring 400 and partially bears on the end face of the first part 110, so that the sealing ring 400 and the first part 110 jointly bear the gravity of the first part 110, thereby reducing the frictional force brought by the sealing ring 400 that needs to be overcome during the rotation of the first part 110. It should not be difficult for those skilled in the art to understand that the storage tank 100 is a metal structural member. Therefore, both the first part 110 and the second part 120 are also metal structural members. The frictional force that needs to be overcome when the first part 110 and the second part 120 are in contact can be reduced by improving the surface smoothness, that is, by machining the mutually contacting surfaces of the first part 110 and the second part 120 to be relatively smooth, so that the frictional force that needs to be overcome for the relative rotation between the first part 110 and the second part 120 is smaller. In some other embodiments, the surface of the second part 120 facing the first part 110 may also be recessed downward.

[0151] In some embodiments, it may also be that the top end face part of the first part 110 is recessed downward so that a stepped surface is formed at the top of the first part 110.

[0152] In some other embodiments, such as Figure 8 As shown, the downward recess of the top end face of the first part 110 may also form an annular groove 1131 at the top of the first part 110, and the sealing ring 400 is arranged in the annular groove 1131. Further, as Figure 8As shown in the figure, the first part 110 includes an inner shell 111, an outer shell 112, and an upper end plate 113. Among them, both the inner shell 111 and the outer shell 112 are shell-like structural members, and the inner shell 111 is located inside the outer shell 112. An insulation chamber is formed between the inner shell 111 and the outer shell 112. The top end face of the first part 110 is located on the surface of the upper end plate 113 facing the second part 120. The upper end plate 113 is a ring-shaped structure. A circular groove 1131 for setting the sealing ring 400 is recessed downward on the surface of the upper end plate 113 facing the second part 120. The inner side of the upper end plate 113 is welded to the inner shell 111, and the outer side is welded to the outer shell 112 to seal the insulation chamber. It is not difficult to understand that the circular groove 1131 is located between the inner side and the outer side of the upper end plate 113. The existence of the circular groove 1131 forms a first convex ring and a second convex ring on the upper end plate 113. The inner shell 111 is in contact welding with the first convex ring, and the outer shell 112 is in contact and welded with the second convex ring. Compared with the implementation manner in which the upper end plate 113 is not provided with the circular groove 1131, the contact welding between the first convex ring and the inner shell 111 can reduce the thickness difference between the inner shell 111 and the upper end plate 113 at the welding position, thereby reducing the large deformation of the inner shell 111 after welding. In some other implementation manners, the inner shell 111 and the outer shell 112 can also be welded to the surface of the upper end plate 113 away from the second part 120 instead of being welded to the inner side or the outer side of the upper end plate 113.

[0153] The insulation chamber between the inner shell 111 and the outer shell 112 plays an insulation role. Its insulation effect can be achieved by adding materials with insulation effects to the insulation chamber, or by evacuating the insulation chamber.

[0154] The second implementation manner of setting the sealing ring 400 in a state with a relatively small compression deformation amount between the first part 110 and the second part 120 is as Figure 12 shown. The biological sample storage device 010 includes a first frame 500. The first part 110 is arranged on the first frame 500. The second part 120 is arranged above the first part 110 and is connected to the first frame 500 by an elastic member 800 to transmit part of the gravity of the second part 120 to the first frame 500, thereby reducing the pressure exerted by the second part 120 on the sealing ring 400. Therefore, the pressure exerted by the sealing ring 400 on the first part 110 is small, and thus the friction force brought by the sealing ring 400 that needs to be overcome during the rotation of the first part 110 is reduced. Among them, the second part 120 can be supported on the first frame 500 by the elastic member 800, that is, as Figure 12As shown, the elastic member 800 is arranged in a compressed state on the side of the second part 120 facing the first part 110; the second part 120 can also be suspended on the first frame 500 by the elastic member 800, that is, the elastic member 800 is arranged in a stretched state on the side of the second part 120 away from the first part 110. It is not difficult to understand that the elastic member 800 is a structure with elasticity, and the elastic member 800 can be a structural member made of an elastic material such as rubber, such as an elastic column made of rubber; the elastic member 800 can also be a structural member with an elastic structure such as a spring. Further, when the elastic member 800 is a spring, a guide post 710 can be arranged on the first frame 500 or the second part 120, and the spring is sleeved on the guide post 710 to limit the telescopic direction of the spring; wherein, the guide post 710 can be understood as a rod-shaped structural member arranged parallel to the rotation axis direction of the first part 110. Further, when the driving member 600 and the second part 120 are only in contact connection, an elastic member 800 can also be arranged between the first frame 500 and the second part 120 to balance the gravity of the second part 120 through the elastic member 800.

[0155] Further, based on the above-described embodiment in which the first part 110 and the second part 120 can move away from each other (including the second part 120 moving adaptively relative to the first part 110 and the second part 120 moving relative to the first part 110 under the action of the driving member 600), a guiding structure can also be arranged between the first part 110 and the second part 120 to guide the relative movement between the first part 110 and the second part 120, so that the relative movement between the first part 110 and the second part 120 is smoother. In some embodiments, the guiding structure includes a guide post 710 and a guide sleeve 720, and one of the guide post 710 and the guide sleeve 720 is arranged on the first frame 500 and the other is arranged on the second part 120. In some other embodiments, the guiding structure can also be a slider and a slide block arranged inside the slider; the guiding structure can also be a guide rail and a slider slidably connected to the guide rail. Further, the spring for balancing the gravity of the second part 120 can also be sleeved outside the guide post 710 that plays a guiding role on this basis.

[0156] For example, in Figure 2 、 Figure 3 and Figure 12In the illustrated embodiment, the biological sample storage device 010 includes a first frame 500. A first part 110 is mounted on the first frame 500, and a second part 120 is located directly above the first part 110 and is slidably connected to the first frame 500 in the vertical direction. A guide post 710 is connected to the side of the second part 120 facing the first part 110, and the first frame 500 is further provided with a guide sleeve 720 that cooperates with the guide post 710. The sliding connection between the first frame 500 and the second part 120 is achieved through the mutually cooperating guide post 710 and guide sleeve 720. Further, the first frame 500 includes a chassis 510 and four columns 520 connected to the same side of the bottom plate 220. The driving unit 300 is mounted on the chassis 510 of the first part 110, and the guide sleeve 720 is provided at the end of the column 520 away from the chassis 510. The guide post 710 is connected to the second part 120 and cooperates with the guide sleeve 720. A spring sleeved on the guide post 710 is further provided between the first frame 500 and the second part 120. One end of the spring contacts the first frame 500 and the other end contacts the second part 120 in the vertical direction, and the spring is in a compressed state. Figure 3 In the illustrated embodiment, since the guide post 710 is connected to the side of the second part 120 facing the first part 110, during the movement of the second part 120, the guide post 710 moves together with the second part 120. Compared with the embodiment in which the guide post 710 is provided on the first part 110, Figure 1 the biological sample storage device 010 of the illustrated embodiment has a smaller height dimension.

[0157] As described above, the carrying unit 200 rotates synchronously with the rotation of the first part 110. In some embodiments, the carrying unit 200 and the first part 110 may be fixedly connected to achieve synchronous rotation of the first part 110 and the carrying unit 200. In some other embodiments, a mutually cooperating structure may also be adopted between the first part 110 and the carrying unit 200 to limit the relative rotation between the first part 110 and the carrying unit 200, thereby achieving synchronous rotation between the first part 110 and the carrying unit 200. Other relative movement modes between the first part 110 and the carrying unit 200 may not be restricted. For example, the first part 110 and the carrying unit 200 are slidably connected through a chute extending along the rotation axis direction of the first part 110 and a slider slidably disposed in the chute. Therefore, when the first part 110 rotates, the carrying unit 200 rotates together, and the movement of the carrying unit 200 in the direction along the rotation axis of the first part 110 is not restricted. Among them, either the slider or the chute can be provided on the first part 110, and the other can be provided on the carrying unit 200.

[0158] Further, in the embodiment where the second part 120 is located above the first part 110 and the carrying unit 200 is fixedly connected to the first part 110 to achieve synchronous rotation, the top of the carrying unit 200 can be fixedly connected to the first part 110, or the middle of the carrying unit 200 can be fixedly connected to the first part 110, or the bottom of the carrying unit 200 can be fixedly connected to the first part 110. Herein, the top, middle, and bottom of the carrying unit 200 are the top, middle, and bottom in the state where the carrying unit 200 is disposed in the cavity. It is not difficult to understand that the storage tank 100 has a closed bottom. In this embodiment, regardless of whether the first part 110 is a tank body or a plate-like structural member, the bottom of the storage tank 100 is located at the first part 110. Therefore, the way of fixedly connecting the bottom of the carrying unit 200 to the first part 110 is applicable to the case where the first part 110 is a tank body, the case where the first part 110 is a plate-like structural member, and the case where the first part 110 has other shapes.

[0159] In some embodiments where the second part 120 is directly above the first part 110, the carrying unit 200 includes a top plate 210, a bottom plate 220, and a connecting member 230 located between the top plate 210 and the bottom plate 220. The connecting members 230 are used to install the basket 1500. After the carrying unit 200 is disposed in the cavity, the top plate 210 of the carrying unit 200 is located above the bottom plate 220 of the carrying unit 200, and the connecting member 230 extends in the vertical direction. The connecting member 230 is used to connect the top plate 210 and the bottom plate 220; the basket 1500 for placing biological samples is disposed in the space between the two connecting members 230. As Figure 10 、 Figure 11 、 Figure 14 and Figure 15 shown, the connecting member 230 is a rod-shaped structural member. In some other embodiments, the connecting member 230 can also be a plate-like structural member, that is, the basket 1500 for placing biological samples is separated by the connecting member 230. The connecting member 230 is preferably an aluminum rod-shaped structural member, which has a lighter weight on the one hand and better heat conduction performance on the other hand, and can make the temperature of the area where the top plate 210 is located closer to the temperature of the area where the bottom plate 220 is located. The top plate 210 is provided with a placement hole 211, and the placement hole 211 is used for the basket 1500 to pass through, and the edge of the placement hole 211 is in concave-convex fit positioning with the top edge of the basket 1500; that is, the upper end of the basket 1500 is positioned at the position of the placement hole 211 of the top plate 210, and the lower end of the basket 1500 passes through the placement hole 211 of the top plate 210 and is located between the top plate 210 and the bottom plate 220; the basket 1500 hangs on the top plate 210 under the action of gravity.

[0160] It is not difficult to understand that since there is a positioning fit between the basket 1500 and the placement hole 211 of the top plate 210, when installing the bearing unit 200, it is necessary to adjust the bearing unit 200 to make the process of loading and unloading the basket 1500 from the placement hole 211 of the top plate 210 smoother. For example, in the embodiment where the basket 1500 is taken out or loaded in the vertical direction, it is necessary to adjust the position of the top plate 210 in the bearing unit 200 so that the basket 1500 can be better fitted with the edge of the placement hole 211 under the action of gravity. In some embodiments, after the bearing unit 200 is loaded into the cavity of the storage tank 100, the adjustment is carried out in the state of the whole bearing unit 200, and then the position of the top plate 210 is adjusted so that the basket 1500 can be smoothly loaded and unloaded in the vertical direction. Specifically, in Figure 11 In the shown embodiment, the placement hole 211 on the top plate 210 is a rectangular hole, and grooves 2111 are provided along the four sides of the placement hole 211; the basket 1500 is a cuboid structural member, and rod-shaped structures protruding outward are provided at the tops of the four side surfaces of the basket 1500. After the basket 1500 is placed into the placement hole 211, the rod-shaped structural members 1520 at the top of the basket 1500 are fitted with the grooves 2111, thereby realizing the concave-convex fit positioning between the edge of the placement hole 211 and the top edge of the basket 1500. When installing the bearing unit 200, when the basket 1500 is hung on the top plate 210 under the action of gravity by adjusting the bearing unit 200, the four rod-shaped structural members 1520 of the basket 1500 can be closely fitted with the four grooves 2111 at the edges of the corresponding placement holes 211, so as to smoothly take out the basket 1500 in the process of taking out the basket 1500 in the vertical direction. In other embodiments, it is also possible to provide grooves 2111 on the basket 1500 and provide rod-shaped structures extending into the placement hole 211 at the edge of the placement hole 211 of the top plate 210, and the basket 1500 is hung on the top plate 210 by the cooperation of the grooves 2111 of the basket 1500 and the rod-shaped structures at the edge of the placement hole 211.

[0161] In order to adjust the bearing unit 200 to make the process of loading and unloading the basket 1500 from the placement hole 211 of the top plate 210 smoother, for Figure 14 and Figure 15In the illustrated embodiment, the bottom plate 220 of the carrying unit 200 is provided with a plurality of first through holes 221 penetrating the plate surface. A fixing member can be provided in each first through hole 221, and each fixing member can independently adjust the distance between the bottom plate 220 and the first part 110, where the distance is the distance in the vertical direction. When the first part 110 is a tank body, that is, the distance between the bottom plate 220 and the bottom wall of the tank body is adjusted by the fixing member. By adjusting the fixing members at different positions, the distances between different positions of the bottom plate 220 and the first part 110 are different, so that the carrying unit 200 can be inclined in the tank body. Correspondingly, the angle between the top plate 210 and the horizontal plane can be changed, so that the rod-shaped structural member 1520 on the side of the basket 1500 can better cooperate with the groove 2111 at the edge of the placing hole 211. Further, the plurality of fixing members are uniformly distributed on the bottom plate 220. In the present application, "plurality" means a number of three or more, that is, the number of the first through holes 221 provided with fixing members on the bottom plate 220 is at least three.

[0162] To achieve the adjustment of the distance between the bottom plate 220 and the bottom wall by the fixing member as described above, a bolt can be used as the fixing member and the fixing member is threadedly connected to the bottom plate 220. Specifically, the first through hole 221 of the bottom plate 220 has a thread, and the bolt is threadedly engaged with the first through hole 221 of the bottom plate 220, and the tail of the bolt abuts against the bottom wall of the tank body, and the head of the bolt is located on the side of the bottom plate 220 facing the top plate 210, so as to screw the bolt into the first through hole 221 of the bottom plate 220 by rotating the head of the bolt to increase the distance between the bottom plate 220 and the bottom wall of the tank body at the position of the bolt; or by screwing the bolt out of the first through hole 221 of the bottom plate 220 to reduce the distance between the bottom plate 220 and the bottom wall of the tank body at the position of the bolt. Further, a nut can be welded at the position of the first through hole 221 on the bottom plate 220, and the threaded hole of the nut is aligned with the first through hole 221 on the bottom plate 220, so that after the tail of the bolt is screwed into the nut, it can pass through the first through hole 221 of the bottom plate 220 and abut against the bottom wall of the tank body. In other embodiments, the bottom plate 220 and the bottom wall of the first part 110 can also be fixed by means of threaded connection or the like.

[0163] It is not difficult to understand that the above-mentioned position adjustment of the bearing unit 200 should be carried out when installing the bearing. Those skilled in the art can connect the bearing unit 200 into an integral body, and then install the bearing unit 200 as a whole into the cavity and adjust the bearing unit 200 through the fixing member; further, through holes can be provided on the top plate 210 corresponding to the positions of the fixing members on the bottom plate 220, so as to facilitate rotating the fixing member through the through holes to adjust the bearing unit 200. It is also possible to first install the bottom plate 220 into the cavity, adjust the position of the bottom plate 220 through the fixing member, and then connect the top plate 210 and the bottom plate 220 through the connecting member 230.

[0164] Further, in some embodiments, in order to make the process of loading and unloading the basket 1500 from the opening 121 of the second part 120 smoother, the position of the top plate 210 is also adjusted. As Figure 16 shown, the first part 110 is a tank body, and a plurality of second through holes 251 are circumferentially arranged in the area of the bearing unit 200 close to the top plate 210. A fixing member can be arranged in each second through hole 251, and the axial direction of each second through hole 251 is perpendicular to the axial direction of the rotation axis of the tank body, so that each fixing member is configured to independently adjust the distance between the bearing unit 200 and the inner peripheral wall of the tank body, thereby enabling the second through holes 251 on the top plate 210 to better align with the opening 121 of the second part 120. Specifically, as Figure 16 shown, a fixing block 250 is connected to the side of the top plate 210 facing the bottom plate 220. The second through hole 251 is arranged in the fixing block 250, and the second through hole 251 is provided with threads. The second fixing member is a bolt that is threadedly engaged with the fixing block 250, and the tail of the bolt contacts the inner peripheral wall of the tank body. By adjusting the fixing members at different positions, the distance between the top plate 210 and the inner peripheral wall of the tank body at this position can be changed. In addition, the fixing members circumferentially arranged on the top of the bearing unit 200 can not only change the position of the top plate 210, but also play a role in tightly pressing against the inner peripheral wall of the tank body, thereby further fixing the bearing unit 200.

[0165] In the embodiment where the second part 120 is arranged directly above the first part 110, after installing the first part 110, the direction of the rotation axis of the first part 110 is also adjusted so that the direction of the rotation axis of the first part 110 is the vertical direction, and then the bearing unit 200 is adjusted. To Figure 15As shown in the structure of the carrying unit 200, both the top plate 210 and the bottom plate 220 in the carrying unit 200 are flat structural members, and the connecting member 230 is perpendicular to the top plate 210 and the bottom plate 220. The placing hole 211 on the top plate 210 penetrates the top plate 210 in a direction perpendicular to the top plate 210. After adjusting the carrying unit 200, the installation position of the carrying unit 200 should meet the following requirements: after the basket 1500 is placed in the placing hole 211, under the action of gravity, the rod-shaped structures on the side of the basket 1500 are all attached to the groove 2111. That is, after adjusting the rotation axis direction of the first part 110 to the vertical direction, the carrying unit 200 is adjusted by the fixing member so that the normal direction of the bottom plate 220 is parallel to the rotation axis direction of the first part 110, or the normal direction of the top plate 210 is parallel to the rotation axis direction of the first part 110, or the axis of the carrying unit 200 is parallel to the rotation axis of the first part 110, then the installation position of the carrying unit 200 can meet the requirements. On this basis, combined with a plurality of fixing members arranged axially on the top of the carrying unit 200, it can also play the role of adjusting the coaxiality between the carrying unit 200 and the first part 110. In some embodiments of the present application, the method of setting through holes and fixing members located in the through holes can also be used to adjust the direction of the rotation axis of the first part 110.

[0166] The above method of adjusting the rotation axis of the first part 110 and the position of the carrying unit 200 by setting fixing members and through holes is applicable not only to the application scenario of taking out the basket 1500 in the vertical direction, but also to the scenario of taking out the basket 1500 in other directions.

[0167] In some embodiments of the present application, the first part 110 is driven to rotate by a gear transmission method. As Figure 22 shown, the driving unit 300 includes a gear ring 320 coaxially connected to the first part 110, and a driving gear 310 meshing with the gear ring 320. The driving gear 310 is arranged on the first frame 500. By connecting the driving gear 310 with the motor 330, the first part 110 is driven to rotate. Among them, the coaxial connection between the gear ring 320 and the first part 110 includes the direct connection between the gear ring 320 and the first part 110, and also includes the indirect connection between the gear ring 320 and the first part 110. In some other embodiments, it is also possible to use Figure 4 and Figure 5 the worm and worm gear structure shown in the figure to drive the first part 110 to rotate. In the figure, the worm 342 is connected to the motor 330, and the worm gear 341 is connected to the first part 110. In some other embodiments, it can also be Figure 31 and Figure 32 shown in the figure that the first part 110 is directly driven by the motor 330 to rotate; orFigure 6 As shown, the existing hollow rotary table is adopted to drive the first part 110 to rotate. Further, the driving gear 310 can be a helical gear. In the case where the driving gear 310 is a helical gear, no matter whether it drives the first part 110 to rotate forward or backward, the rotation accuracy of the first part 110 can be relatively high.

[0168] Further, the transmission connection position between the driving unit 300 and the first part 110 can be Figure 2 as shown, at the bottom of the first part 110, or can be Figure 5 as shown, in the middle of the first part 110, or can also be Figure 4 as shown, at the top of the first part 110.

[0169] In order to overcome problems such as insufficient rotation accuracy and unstable rotation of the first part 110 caused by the assembly gap between the driving gear 310 and the gear ring 320, a force can also be applied to the driving gear 310 towards the gear ring 320, so that the driving gear 310 and the gear ring 320 are meshed more tightly. Specifically, as Figure 22 shown, the first part 110 is rotatably arranged on the first frame 500. A guide rail 360 extending towards the first part 110 is also arranged on the first frame 500. A sliding seat 350 is slidably arranged on the guide rail 360. The driving gear 310 is rotatably connected to the sliding seat 350. An elastic member 800 in a compressed state is arranged between the sliding seat 350 and the first frame 500 to apply a force to the sliding seat 350 towards the first part 110, so that the driving gear 310 can be tightly meshed with the gear ring 320 connected to the first part 110. Among them, the elastic member 800 can be a structural member with an elastic structure such as a spring, or can also be a structural member such as an elastic column made of an elastic material such as rubber. Further, the motor 330 for driving the driving gear 310 to rotate is also installed on the sliding seat 350.

[0170] In the embodiment of using gear transmission to drive the first part 110 to rotate, in order to enable the motor 330 to more easily drive the first part 110 to rotate, the transmission ratio of the gear transmission is 3 - 10, that is, the transmission ratio can be 3, 4, 5... 10.

[0171] In some embodiments of the present application, in order to reduce the space occupied by the storage device, the first part 110 is a tank body, and the diameter of the side of the first part 110 away from the second part 120 is smaller. The driving unit 300 is arranged on the side of the first part 110 away from the second part 120, and in the direction of the rotation axis of the first part 110, the projection of the driving unit 300 is located within the contour of the projection of the first part 110. It is not difficult to understand that the tank body is a solid of revolution, that is, the diameter of the side of the first part 110 away from the second part 120 is smaller. By arranging the driving unit 300 on the smaller-sized side of the first part 110 and the projection of the driving unit 300 being located within the contour of the projection of the first part 110, the space occupied by the biological sample storage device 010 can be reduced.

[0172] In some embodiments of the present application, a bearing is provided between the first part 110 and the first frame 500 to facilitate the rotation of the first part 110. Among them, in Figure 22 the embodiment shown, the inner ring of the bearing is fixedly connected to the first frame 500, the outer ring 1010 of the bearing is connected to the first part 110, and the gear ring 320 is coaxially connected to the outer ring 1010 of the bearing. Among them, the connection method between the gear ring 320 and the outer ring 1010 can be carried out by an integral molding method, that is, during the manufacturing process of the outer ring 1010, teeth are directly machined on the outer periphery of the outer ring 1010, and the boundary between the gear ring 320 and the outer ring 1010 is an integral structural member; the gear ring 320 and the outer ring 1010 can also be separately machined and then connected. In other embodiments, it can also be that the inner ring of the bearing is connected to the first part 110 and the outer ring 1010 of the bearing is connected to the first frame 500. In the embodiment where the gear ring 320 is connected to the outer ring 1010 of the bearing rather than directly to the first part 110, the processing accuracy requirements for the first part 110 are relatively low, the processing cost can be reduced, and the increase in processing difficulty caused by the larger diameter of the first part 110 can also be avoided, so the production cost is lower.

[0173] Furthermore, in the embodiment where the first part 110 is connected to the outer ring 1010 of the bearing, the first part 110 is preferably a solid of revolution structure such as a tank body. Therefore, in order to more conveniently adjust the axial direction of the first part 110, such as Figure 21As shown, at one end of the first part 110 away from the second part 120, a connecting ring 1100 is further connected. The first part 110 bears against the outer ring 1010 of the bearing through the connecting ring 1100. And a plurality of third through holes 1110 extending along the rotation axis direction of the first part 110 are provided on the connecting ring 1100, and a fixing member for independently adjusting the distance between the connecting ring 1100 and the outer ring 1010 of the bearing is provided in each third through hole 1110. That is, the first part 110 is adjusted in the same way as adjusting the position of the bearing unit 200 in the cavity. For example, when the second part 120 is disposed directly above the first part 110, the axis of the first part 110 is adjusted by the fixing member so that the axis of the first part 110 is parallel to the vertical direction, that is, the axis of the first part 110 coincides with the rotation axis of the first part 110 (it is not difficult to understand that when the second part 120 is disposed directly above the first part 110, when the first part 110 rotates along a preset trajectory, the axis of the first part 110 should coincide with its rotation axis). Specifically, the third through holes 1110 on the connecting ring 1100 are distributed in a circumferential manner and are threaded holes, and the fixing member is a bolt in the prior art. The bolt is in threaded cooperation with the third through hole 1110 on the connecting ring 1100, and the tail of the bolt abuts against the outer ring 1010 of the bearing. By screwing in the fixing member, the distance between the connecting ring 1100 and the outer ring 1010 of the bearing at the position where the fixing member is located can be increased; or the fixing member is screwed out to reduce the distance between the connecting ring 1100 and the outer ring 1010 of the bearing at the position where the fixing member is located. By screwing in or out the fixing member, the axis of the first part 110 can be changed relative to the axis in the vertical direction, so that the axis of the first part 110 is parallel to the rotation axis of the first part 110 and both extend along the vertical direction. Further, the number of the third through holes 1110 provided with the fixing members on the connecting ring 1100 is at least three. In an embodiment where the number of the third through holes 1110 provided with the fixing members is greater than three, the third through holes 1110 provided with the fixing members may not be distributed along the same circumference, but at least three third through holes 1110 are not on the same straight line.

[0174] In the above embodiment, it is not difficult to understand that the connecting ring 1100 is a ring structure. On the one hand, the outer shape of the connecting ring 1100 can be adapted to the bearing, so that it only contacts the outer ring 1010 of the bearing and does not contact the inner ring of the bearing; on the other hand, the overall weight of the biological sample storage device 010 can be reduced. In some other embodiments, it may also be that a plate-shaped structural member is connected to one end of the first part 110 away from the second part 120. Further, a sunken surface may be provided on the plate-shaped structure to avoid contacting the inner ring of the bearing.

[0175] Further, in order to more reliably connect the first part 110 to the outer ring 1010 of the bearing, threaded holes are also provided in the outer ring 1010 of the bearing. The connecting ring 1100 of the first part 110 is locked to the outer ring 1010 of the bearing by a bolt screwed into the threaded hole. Further, a pressing block 1120 can be provided between the head of the bolt and the connecting ring 1100 to increase the contact area with the connecting ring 1100.

[0176] In an embodiment where the second part 120 is located above the first part 110, the first part 110 is a tank body, and the second part 120 is a cover body, a heat-insulating layer 1200 is provided on the side of the cover body facing the tank body. It is not difficult to understand that in order to reduce the weight of the cover body, the thickness of the cover body is relatively thin. By providing the heat-insulating layer 1200 on the side of the cover body facing the tank body, the loss of cold in the chamber through the cover body can be reduced. Further, the heat-insulating layer 1200 can be a structural member obtained by foaming a heat-insulating material. Similarly, a layer of heat-insulating material can also be provided on the side of the sampling cover 1600 facing the cavity.

[0177] When the biological sample storage device 010 provided in the present application is applied to ultra-low temperature storage, the temperature in the cavity needs to reach -196°C, and the refrigeration method in the cavity is liquid nitrogen refrigeration. In order to add liquid nitrogen into the cavity, as Figures 18 to 20 shown, a liquid nitrogen delivery pipe 1300 is also provided in the heat-insulating layer 1200. The liquid nitrogen delivery pipe 1300 has a liquid outlet facing the cavity. The liquid nitrogen delivery pipe 1300 is connected to a liquid nitrogen supply device to introduce liquid nitrogen into the cavity. During use, the external liquid nitrogen supply device delivers liquid nitrogen into the cavity through the liquid nitrogen delivery pipe 1300, and the liquid nitrogen flows into the cavity through the liquid outlet. Further, in some embodiments, the liquid outlet of the liquid nitrogen delivery pipe 1300 is an atomizing port, so that the liquid nitrogen is atomized through the atomizing port and then enters the cavity; the biological sample storage device 010 with an atomizing port at the liquid outlet can be applied to a storage environment of -80°C. It is not difficult to understand that in the embodiment where the liquid outlet is an atomizing port, the size of the liquid outlet is relatively small; in some other embodiments, the size of the liquid outlet can also be relatively large, so that the liquid nitrogen can still flow downward in a water flow shape to the bottom of the cavity after passing through the liquid outlet, so that the temperature in the cavity can reach -196°C.

[0178] Further, in order to make the liquid nitrogen flow more evenly through the carrier unit 200 after flowing out of the liquid outlet, in some embodiments, the liquid nitrogen delivery pipe 1300 includes an arc section 1310 and a straight section 1320 connected to the arc section 1310. The straight section 1320 extends along the radial direction of the arc section 1310, and liquid outlets are provided on both the arc section 1310 and the straight section 1320. It is not difficult to understand that the liquid outlets provided on the arc section 1310 can enable the liquid nitrogen to pass through the carrier unit 200 from multiple positions on the circumference, and the liquid outlets provided on the straight section 1320 can enable the liquid nitrogen to pass through the carrier unit 200 from multiple positions in the radial direction. Further, the liquid outlets are evenly distributed on the arc section 1310 and the straight section 1320. In some embodiments, the arc section 1310 may further include two inner and outer sections with different diameters. The two inner and outer arc sections 1310 are coaxially arranged and connected by the straight section 1320.

[0179] In some other embodiments, the temperature in the cavity is only required to be -80°C. In this embodiment, a compression refrigeration system in the prior art can be used for refrigeration. Specifically, the evaporator in the compression refrigeration system is connected to the side of the second part 120 facing the cavity to perform heat exchange with the inside of the cavity, so that the temperature in the cavity reaches -80°C. In the embodiment using the compression refrigeration system for refrigeration, the second part 120 may not be located above the first part 110.

[0180] In some embodiments, a temperature sensor 1420 is further provided to detect the temperature in the cavity, so as to adjust the temperature in the cavity according to the temperature change in the cavity. Preferably, three temperature sensors 1420 are provided in the cavity, and the three temperature sensors 1420 are arranged in the vertical direction to detect the temperatures at different height positions in the cavity.

[0181] Further, in the embodiment using liquid nitrogen for refrigeration, the temperature in the cavity can be controlled by controlling the amount of liquid nitrogen in the cavity. Therefore, a liquid level sensor 1410 can also be provided in the cavity to detect the liquid level height in the cavity, so as to determine whether it is necessary to add liquid nitrogen to the cavity.

[0182] Therefore, in the embodiment where the second part 120 is located above the first part 110 and the cavity is refrigerated with liquid nitrogen, the biological sample storage device 010 further includes a sensor assembly 1400, such as Figures 23 to 25As shown, the sensor assembly 1400 includes a liquid level sensor 1410 and a temperature sensor 1420. The first part 110 is a tank body, and the bottom of the first part 110 is a curved surface protruding away from the cavity, so that liquid nitrogen can converge towards the center of the bottom wall of the tank body in the cavity. The bearing unit 200 includes a bottom plate 220 at the bottom. The bottom plate 220 is a flat structural member. A gap for accommodating liquid nitrogen is formed between the bottom plate 220 and the bottom wall of the tank body. The detection end of the liquid level sensor 1410 is located in this gap.

[0183] In the embodiment provided by the present application, the first part 110 rotates relative to the second part 120 under the action of the driving unit 300, and the bearing unit 200 rotates with the first part 110, and the second part 120 is fixed relative to the first frame 500. Therefore, a rotating shaft is not provided in the central part of the bearing unit 200, nor is a rotating shaft provided in the central part of the cavity. Therefore, the sensor assembly 1400 provided by the present application can be disposed at the central position of the cavity, that is, the rotation axis position of the first part 110. Correspondingly, a detection channel 240 with both ends penetrating is provided at the rotation axis of the bearing unit 200 to avoid the sensor assembly 1400, that is, one end of the detection channel 240 penetrates the upper end of the bearing unit 200, and the other end penetrates the lower end of the bearing unit 200. The sensor assembly 1400 passes through the detection channel 240, and the detection end of the liquid level sensor 1410 extends into the gap between the bottom plate 220 and the bottom wall of the tank body.

[0184] It is not difficult to understand that liquid nitrogen converges at the bottom wall of the tank body, and the lowest liquid level of the liquid nitrogen is also located at the central position of the cavity, that is, the rotation axis position of the first part 110. Therefore, by disposing the sensor assembly 1400 at the rotation axis position of the first part 110, the liquid level height of the liquid nitrogen can be better detected, that is, the detection range of the sensor is larger. On the other hand, the sensor assembly 1400 is installed on the second part 120, and the signal line 1460 of the liquid level sensor 1410 and the signal line 1460 of the temperature sensor 1420 pass out from the second part 120. That is, the bearing unit 200 rotates relative to the sensor assembly 1400. Therefore, passing the sensor through the detection channel 240 to detect the liquid level of the liquid nitrogen and the temperature in the cavity can not only facilitate the setting of the bearing assembly and avoid excessive interference between the sensor assembly 1400 and the bearing assembly, but also facilitate the extraction of the signal line 1460 of the sensor. Further, the sensor assembly 1400 includes a mounting rod 1450. The mounting rod 1450 passes through the detection channel 240 and extends into the gap between the bottom plate 220 of the bearing unit 200 and the bottom wall of the tank body. The liquid level sensor 1410 is disposed at the end of the mounting rod 1450 located in this gap.

[0185] To facilitate the connection of the sensor assembly 1400 to the second part 120, the sensor assembly 1400 further includes a connecting plate 1430 and a cylindrical structural member 1440 that are interconnected. The connecting plate 1430 is a flange-shaped structural member. The connecting plate 1430 and the mounting rod 1450 are respectively connected to two opposite ends of the cylindrical structural member 1440. The connecting plate 1430 is connected to the second part 120. In some embodiments, that is, the connecting plate 1430 is connected to the cover body. The cylindrical structural member 1440 is located within the thermal insulation layer 1200, and the mounting rod 1450 extends away from the thermal insulation layer 1200 until it extends into the gap between the bottom plate 220 and the bottom wall of the tank body. The temperature sensors 1420 are evenly distributed on the mounting rod 1450.

[0186] Furthermore, the connecting plate 1430 is connected to the side of the first part 110 facing away from the thermal insulation layer 1200, and the cylindrical structural member 1440 extends away from the first part 110 until it is flush with the side of the thermal insulation layer 1200 facing the cavity. The connecting plate 1430 has a first opening 121, and the cylindrical structural member 1440 has a second opening 121. The first opening 121 and the second opening 121 are aligned. The signal lines 1460 of the liquid level sensor 1410 and the temperature sensor 1420 pass through the second opening 121 of the cylindrical structural member 1440. In some embodiments, the cross-sectional profile of the cylindrical structural member 1440 encloses the mounting rod 1450 inside, where the cross-section is a cross-section perpendicular to the extending direction of the cylindrical structural member 1440. In some embodiments, thermal insulation materials are also arranged inside the cylindrical structural member 1440 by means of a foaming process through the first opening 121 and the second opening

[0187] Furthermore, the cylindrical structural member 1440 and the connecting plate 1430 are connected by welding, and the cylindrical structural member 1440 and the mounting rod 1450 are connected by threading. Specifically, one end of the mounting rod 1450 is a threaded section, and the threaded section is located inside the cylindrical structural member 1440, and the top end of the threaded section is lower than the surface of the connecting plate 1430 facing away from the cylindrical structural member 1440. A nut is connected to the threaded section to connect the mounting rod 1450 and the cylindrical structural member 1440. That is, on the one hand, the mounting rod 1450 can be suspended inside the cylindrical structural member 1440 by the nut, and on the other hand, the mounting rod 1450 and the nut can clamp the cylindrical structural member 1440 together to relatively fix the cylindrical structural member 1440 and the mounting rod 1450. In some other embodiments, the mounting rod 1450 and the cylindrical structural member 1440 can also be directly connected by welding.

[0188] The carrying unit 200 has a plurality of placement positions distributed circumferentially, and the biological sample is arranged at the placement position of the carrying unit 200. In the embodiment where the carrying unit 200 includes the top plate 210, the placement holes 211 on the top plate 210 are correspondingly arranged at the placement positions of the carrying unit 200. During the rotation of the storage tank 100, the carrying positions on the carrying unit 200 pass below the opening 121 of the second part 120. When the carrying position is below the opening 121, the biological sample arranged at the corresponding placement position can be sampled.

[0189] In some embodiments, in the projection along the rotation direction of the storage tank 100, the placement positions are distributed on the same circumference. Correspondingly, the number of openings 121 on the second part 120 is one.

[0190] In some other embodiments, as Figure 11 shown in Figure 14 In the projection along the rotation direction of the storage tank 100, the placement positions are arranged in at least two circles, specifically two or three circles. The number of openings 121 on the second part 120 can be two or one. When the number of openings 121 on the second part 120 is one, the opening 121 extends in the radial direction of the lid body (i.e., the second part 120), so that after the tank body (i.e., the first part 110) rotates, the biological samples at different placement positions can be taken out from the opening 121. Among them, the lid body can be rectangular or other shapes in addition to being circular. The radial direction of the lid body is the direction on the lid body away from the rotation axis of the first part 110. Further, the placement positions on the inner circle are staggered from the placement positions on the outer circle 1010 to better allocate the space for placing biological samples.

[0191] The biological sample storage system provided by this application includes a second rack 030, an access unit 020, and any of the aforementioned biological sample storage devices 010. The first rack 500 is connected to the second rack 030. The access unit 020 is disposed on the second rack 030, and the access unit 020 is located on the side of the second part 120 away from the first part 110. The access unit 020 can open the sampling cover 1600 covering the opening 121 of the second part 120, and then the sampling part of the access unit 020 extends into the cavity to take the biological sample. When the number of openings 121 on the second part 120 is only one, only one sampling cover 1600 is required, and for the biological samples corresponding to the placement positions on different circumferences, the operation of opening the sampling cover 1600 during the sampling process is the same, which can make the structure of the access unit 020 relatively simple. In the embodiment where the placement positions are at least distributed in two circles in the projection along the rotation direction of the storage tank 100, if an opening 121 is provided for each circle of placement positions on the second part 120 and a separate sampling cover 1600 is provided for each opening 121, it will cause the sampling mechanism to need to be able to open the sampling covers 1600 at multiple positions, making the structure of the sampling mechanism more complex. In some embodiments, the access unit 020 can also be directly installed on the first rack 500.

[0192] Furthermore, in some embodiments, the second rack 030 is detachably connected to the storage tank 100, and the second rack 030 is connected with an adjustable support frame 040. Through the adjustable support frame 040, the second rack 030 can be separated from the storage tank 100, and then the storage tank 100 can be taken out. When the storage tank 100 is installed on the first rack 500, that is, the second rack 030 is detachably connected to the first rack 500. Through the adjustable support frame 040, the second rack 030 can be separated from the first rack 500, and then the storage tank 100 can be taken out together with the first rack 500.

[0193] In some embodiments, the adjustable support frame 040 can lift the second frame 030 from the ground in a manner that separates the first frame 500 from the second frame 030, so as to separate the second frame 030 from the first frame 500. For example, when the second support 030 is carried on the first frame 500, separating the first frame 500 from the second frame 030 can be achieved by lifting the second frame 030 from the ground. Herein, the adjustable support frame 040 lifting the second frame 030 from the ground means that one end of the adjustable support frame 040 contacts the ground and holds the second frame 030 at a position with a certain distance from the ground, and the distance between the second frame 030 and the ground can be adjusted. The adjustable support frame 040 can be a structure composed of a screw and a nut, that is, the nut is fixedly connected to the second frame 030, the screw is vertically arranged and cooperates with the nut, and by rotating the nut, the screw moves towards the direction of the ground, thereby realizing lifting the second frame 030 from the ground. The adjustable support frame 040 can also adopt other lifting devices in the prior art, such as a jack and other structures.

[0194] Please refer to Figure 29 and Figure 30 , when a failure occurs in structures such as the access unit 020 of the biological sample storage system and automatic sampling cannot be performed through the access unit 020, the connection between the first frame 500 and the second frame 030 can be released. Then, the second frame 030 is lifted from the ground by the adjustable support frame 040 to separate the first frame 500 from the second frame 030. Further, the biological sample storage device 010 can be separated from the access unit 020. For the separated biological sample storage device 010, manual sampling can be used. Further, to facilitate separating the biological sample storage device 010, rollers can also be provided below the first frame 500, and then the first frame 500 together with the storage tank 100 installed on the first frame 500 can be dragged out. Further, as Figure 1 shown, the access unit 020 can further include an open - cover assembly 0210 for grasping the sampling cover 1600. The open - cover assembly 0210 is arranged on the second frame 030 and can move along the X, Y, and Z axes to grasp the sampling cover 1600 and move it away. The access unit 020 can further include a cold trap 0220. The cold trap 0220 also has a refrigeration unit to keep the inside of the cold trap 0220 at a lower temperature. The cold trap 0220 has an extraction assembly for grasping the basket 1500 to lift the basket 1500 in the storage tank 100 into the cold trap 0220. The cold trap 0220 is also movably connected to the second frame 030 to move above the opening 121 of the second part 120 after the open - cover assembly 0210 moves the sampling cover 1600 away.

[0195] In some embodiments, the access unit 020 includes an existing mechanism for sampling. For example, a three-axis manipulator is used for sampling. Further, the sampling process includes: first, the driving unit 300 rotates the first part 110 to rotate the carrier unit 200 fixed on the first part 110. At this time, the second part 120 does not rotate, so that the biological sample to be sampled on the carrier unit 200 rotates to a position corresponding to the opening 121 of the second part 120. Then, the sampling cover 1600 provided at the opening 121 of the second part 120 is removed by the cover opening assembly 0210. Next, the cold trap 0220 is moved above the opening 121, and the extraction assembly extends into the cavity from the opening 121 to take out the basket 1500 provided on the carrier unit 200 into the cold trap 0220. After that, the biological sample placed in the basket 1500 is taken out by the three-axis manipulator.

[0196] The foregoing are only the preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, various modifications and changes can be made to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A biological sample storage device, characterized in that, including a carrier unit for carrying a sample a storage tank; the storage tank includes a first part and a second part that can rotate relative to each other; the first part and the second part are closed to form a cavity for accommodating the carrier unit, and the carrier unit is connected to the first part; an opening for the sample to pass through is provided on the second part a driving unit, which is located outside the cavity and is in transmission connection with the first part to drive the first part and the carrier unit to rotate relative to the second part 2. The biological sample storage device according to claim 1, wherein, The first part is a tank body and the second part is a cover body 3. The biological sample storage device according to claim 2, wherein The second part is arranged above the first part, a sealing ring is arranged between the first part and the second part, and the second part can move in a direction away from or close to the first part 4. The biological sample storage device according to claim 3, characterized in that, It further includes a first frame, the first part is installed on the first frame, the second part is slidably arranged on the first frame, and when the first part rotates, the second part is configured to adaptively move in a direction away from or close to the first part 5. The biological sample storage device according to claim 3, wherein, It further includes a first frame, and a driving member is arranged on the first frame, and the driving member is connected to the second part to drive the second part to move in a direction away from or close to the first part 6. The biological sample storage device according to claim 5, wherein, The driving member is configured to: be able to drive the second part to move between a first position and a second position along the rotation axis direction of the first part, and the second part contacts the sealing ring both at the first position and the second position 7. The biological sample storage device according to claim 6, wherein, It further includes a sensor for detecting the distance between the first part and the second part, the sensor is in signal connection with the driving member, and the driving member is configured to drive the second part to be in a state of compressing the sealing ring according to the signal of the sensor 8. The biological sample storage device according to any one of claims 4-7, characterized in that, A guiding structure is further connected between the second part and the first frame, and the guiding structure includes a guide post and a guide sleeve that are slidably matched; the extending direction of the guide post is parallel to the rotation axis of the first part; one of the guide post and the guide sleeve is arranged on the first frame and the other is arranged on the second part 9. The biological sample storage device according to any one of claims 4-7, characterized in that, An elastic member is connected between the second part and the first frame, and the elastic member is arranged in a compressed state on the side of the second part facing the first part; or is arranged in a stretched state on the side of the second part away from the first part 10. The biological sample storage device according to claim 9, wherein The elastic member is an elastic column Or, the elastic member is a spring, and the spring is sleeved on the guide post 11. The biological sample storage device according to claim 3, characterized in that, The sealing ring is a C-shaped sealing ring, and an installation ring is further included. The installation ring is located between the first part and the second part, and both sides of the C-shaped sealing ring are compressed between the first part and the installation ring, and between the second part and the installation ring respectively 12. The biological sample storage device according to claim 3, wherein The top end face part of the first part is recessed downward, the sealing ring is arranged in the recessed area and the upper part protrudes from the top end face, and the second part compresses the sealing ring under the action of gravity and partially bears on the top end face 13. The biological sample storage device according to claim 12, characterized in that, The first part includes an inner shell, an outer shell and an upper end plate. An insulating chamber is formed between the inner shell and the outer shell. The surface part of the upper end plate facing the second part is recessed downward to form an annular groove for arranging the sealing ring. The inner side of the upper end plate is welded to the inner shell, and the outer side is welded to the outer shell to seal the insulating chamber.

14. The biological sample storage device according to claim 3, wherein The bottom of the bearing unit is fixed to the tank body.

15. The biological sample storage device according to claim 14, characterized in that, The bearing unit includes a bottom plate. The bottom plate is provided with a plurality of through holes penetrating the plate surface. Each through hole can be provided with a fixing member, and each fixing member is configured to independently adjust the distance between the bottom plate and the bottom wall of the tank body.

16. The biological sample storage device according to claim 15, wherein The through hole is provided with threads, and the fixing member is a bolt threadedly engaged with the through hole. The tail of the bolt contacts the bottom wall of the tank body.

17. The biological sample storage device according to claim 15, characterized in that, The bearing unit further includes a top plate. A plurality of connecting members are connected between the bottom plate and the top plate. A basket is installed between the plurality of connecting members. One end of the connecting member is connected to the bottom plate, and the other end is connected to the top plate.

18. The biological sample storage device according to claim 17, wherein, The connecting member is an aluminum rod-shaped structural member.

19. The biological sample storage device according to claim 17, wherein, The top plate is provided with a placement hole for the basket to pass through. The edge of the placement hole is in concave-convex fit with the top edge of the basket for positioning.

20. The biological sample storage device according to claim 17, wherein A plurality of through holes are circumferentially provided in the area of the bearing unit close to the top plate. Each through hole can be provided with a fixing member, and each fixing member is configured to independently adjust the distance between the bearing unit and the inner peripheral wall of the tank body.

21. The biological sample storage device according to claim 20, wherein The through hole is provided with threads, and the fixing member is a bolt threadedly engaged with the through hole. The tail of the bolt contacts the inner peripheral wall of the tank body.

22. The biological sample storage device according to any one of claims 1-3, characterized in that, A bearing is connected between the bottom of the first part and the first frame. The inner ring of the bearing is fixedly connected to the first frame, and the outer ring of the bearing is connected to the first part.

23. The biological sample storage device according to claim 22, wherein A connecting ring is connected to one end of the first part away from the second part. The connecting ring is provided with a plurality of through holes extending in the direction of the rotation axis. Each through hole can be provided with a fixing member, and each fixing member is configured to independently adjust the distance between the connecting ring and the outer ring of the bearing.

24. The biological sample storage device according to claim 23, wherein, The through hole is provided with threads, and the fixing member is a bolt threadedly engaged with the through hole. The tail of the bolt contacts the upper surface of the outer ring of the bearing.

25. The biological sample storage device according to claim 22, wherein The outer ring of the bearing is provided with teeth. The driving unit includes a driving gear, and the driving gear meshes with the teeth of the outer ring.

26. The biological sample storage device according to claim 25, characterized in that, The first frame is provided with a guide rail. A sliding seat is arranged on the guide rail. The driving gear is installed on the sliding seat. An elastic member in a compressed state is arranged between the sliding seat and the first frame to apply a force towards the first part to the sliding seat.

27. The biological sample storage device according to claim 26, wherein The elastic member is a spring or an elastic column.

28. The biological sample storage device according to claim 25, characterized in that, The driving gear is a helical gear.

29. The biological sample storage device according to claim 25, characterized in that, The transmission ratio between the driving gear and the outer ring is 3-10.

30. The biological sample storage device according to claim 25, wherein, The driving unit is located on the side of the first part away from the second part. In the direction of the rotation axis of the first part, the projection of the driving unit is located within the contour of the projection of the first part.

31. The biological sample storage device according to claim 3, wherein, The cover body is a plate-shaped structural member, and a heat-insulating layer is connected to a side of the cover body facing the tank body.

32. The biological sample storage device according to claim 31, wherein A liquid nitrogen delivery pipe is also provided in the thermal insulation layer, and the liquid nitrogen delivery pipe is provided with a liquid outlet facing the cavity.

33. The biological sample storage device according to claim 32, characterized in that, The liquid nitrogen delivery pipe includes an arc segment and a straight segment connected to the arc segment; the arc segment and the straight segment are both provided with the liquid outlet.

34. The biological sample storage device according to claim 32, wherein, The liquid nitrogen delivery pipe is also connected to a liquid nitrogen supply device; and the liquid outlet is an atomization outlet.

35. The biological sample storage device according to any one of claims 32-34, characterized in that, The carrying unit includes a bottom plate, and a gap for accommodating liquid nitrogen is defined between the bottom plate and the bottom wall of the tank body; the biological sample storage device also includes a sensor assembly, which includes a liquid level sensor, and a detection end of the liquid level sensor is located in the gap.

36. The biological sample storage device according to claim 35, wherein, A detection channel with two ends extending therethrough is provided at the rotation axis of the carrying unit; the sensor assembly includes a mounting rod, the mounting rod passes through the detection channel and extends into the gap, and the liquid level sensor is provided at the end of the mounting rod.

37. The biological sample storage device according to claim 36, characterized in that, The sensor assembly also includes a connecting disk and a cylindrical structure that are interconnected, the connecting disk is connected to the cover body, the cylindrical structure is located in the insulation layer, and the mounting rod is connected to one end of the cylindrical structure away from the connecting disk and extends in a direction away from the insulation layer; the sensor assembly also includes a temperature sensor, and the temperature sensor and the liquid level sensor are both arranged on the mounting rod.

38. The biological sample storage device according to claim 37, wherein, The connecting disk has a first opening, the cylindrical structure has a second opening, the first opening is aligned with the second opening, and the signal lines of the liquid level sensor and the temperature sensor pass through the second opening and the first opening.

39. The biological sample storage device according to claim 37, wherein, One end of the mounting rod is a threaded section, and the threaded section is located in the cylindrical structure. A nut is provided in the cylindrical structure and connected to the threaded section to connect the mounting rod to the cylindrical structure.

40. The biological sample storage device according to claim 3, characterized in that, The carrying unit has a plurality of placement positions distributed along the circumference, and the biological samples are set at the placement positions; during the rotation of the storage tank, the placement positions pass under the opening.

41. The biological sample storage device according to claim 40, wherein, In the projection along the rotation direction of the storage tank, the placement positions are arranged in at least two circles; The number of the opening is one, and the opening extends along the radial direction of the second part, so that after the first part is rotated, biological samples at different positions can be taken out from the opening.

42. A biological sample storage system, characterized in that, The biological sample storage device comprises an access unit and any one of claims 1-41, wherein the access unit is located on a side of the second part away from the first part; the access unit can be moved to the opening to access the sample from the opening.

43. The biological sample storage system according to claim 42, wherein It also includes a second frame, the access unit is installed on the second frame; the second frame is detachably connected to the storage tank, the second frame is connected to an adjustable support frame, and the adjustable support frame is configured to separate the second frame from the storage tank.

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