Sampler and detection equipment

Abstract

The utility model discloses a sampler and detection equipment, the sampler comprises: a plurality of cuvettes, each cuvette is provided with an accommodating cavity and a reaction cavity which are communicated, and the side cavity wall of the reaction cavity is light-transmitting; the reaction cavity is used for containing a first detection reagent; the liquid storage tube is detachably connected with the containing cavity, a sealing film is arranged at an opening of the liquid storage tube, and the liquid storage tube is used for containing a second detection reagent; the capillary tube element comprises a first tube body, a second tube body and a sampling head, the first tube body, the second tube body and the sampling head are sequentially connected and communicated, and the second tube body is provided with a pressure adjusting port; the sampling head is used for collecting a to-be-detected sample; the material pushing device comprises a material pushing barrel; the material pushing barrel is detachably connected with the first pipe body; the pushing component is used for pushing the capillary element to move towards the liquid storage tube, so that the sampling head punctures the sealing film, and the second detection reagent and the to-be-detected sample flow into the reaction cavity. According to the scheme, simpler sampling operation and detection operation can be provided, so that the accuracy and reliability of home autonomous detection are guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of sampling, in particular to a sampler. Background Art

[0002] Traditional medical tests typically require in-person visits to healthcare facilities. However, with the continued growth of the population, the burden on medical resources is increasing. To address this, home testing technologies have been developed to alleviate this burden. Currently, immunochromatographic test strips are widely used for home testing due to their simple process and low cost. Immunochromatographic test strips are a rapid, immune-based test technology that binds to the test substance via specific antibodies, producing a visual result. In preliminary home self-testing, this technology has demonstrated advantages in detecting specific biomarkers in samples such as urine, saliva, and blood. However, while immunochromatographic test strips hold promise for home testing, they also have limitations. When using test strips for self-testing, users must adhere to specific procedures and time limits. For example, sample collection and processing, as well as the immersion time of the test strip, require precise control. Any operational deviation can lead to inaccurate test results. Consequently, the actual operation of the test strips is complex, making it difficult to guarantee the accuracy and reliability of home self-testing. Utility Model Content

[0003] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention proposes a sampler that can provide simpler sampling and detection operations to ensure the accuracy and reliability of self-detection at home.

[0004] According to a first embodiment of the present invention, a sampler is provided, comprising:

[0005] A plurality of cuvettes are provided with a communicating receiving cavity and a reaction cavity, wherein the side walls of the reaction cavity are light-transmissive; the reaction cavity is used to hold a first detection reagent;

[0006] a liquid storage tube, detachably connected to the accommodating cavity, wherein an opening of the liquid storage tube is provided with a sealing film, and the liquid storage tube is used to hold a second detection reagent;

[0007] The capillary element comprises a first tube body, a second tube body and a sampling head, wherein the first tube body, the second tube body and the sampling head are sequentially connected and communicated, and the second tube body is provided with a pressure regulating port; the sampling head is used to collect a sample to be tested;

[0008] The pushing device includes a pushing cylinder; the pushing cylinder is detachably connected to the first tube body; and is used to push the capillary element toward the liquid storage tube so that the sampling head pierces the sealing membrane, allowing the second detection reagent and the sample to be tested to flow into the reaction chamber.

[0009] The sampler provided by the embodiment of the first aspect of the utility model has at least the following beneficial effects: a plurality of cuvettes are provided in the sampler, each cuvette is provided with a connected accommodating chamber and a reaction chamber, a first detection reagent is contained in the reaction chamber, and the side walls of the reaction chamber are light-transmissive so that the light signals before and after the chemical reaction can be captured by the detection equipment for subsequent detection and analysis; the accommodating chamber is detachably connected to the liquid storage tube, and the opening of the liquid storage tube is provided with a sealing film, so that a second detection reagent can be contained and stored; a pushing cylinder in the pushing device is detachably connected to the first tube body of the capillary element, and the sampling head of the capillary element can collect the sample to be tested by capillary action, eliminating the complicated sampling operation steps and reducing the difficulty of sampling; after the collection is completed, the pushing cylinder is pushed to push the capillary element toward the liquid storage tube, so that the sampling head pierces the sealing film of the liquid storage tube, so that the second detection reagent and the sample to be tested flow into the reaction chamber to produce a chemical reaction with the first detection reagent, and the detection is achieved without the need for complicated detection operations, thereby ensuring the accuracy and reliability of home self-testing. That is to say, the utility model can provide simpler sampling and detection operations to ensure the accuracy and reliability of self-detection at home.

[0010] According to some embodiments of the present invention, the number of the cuvettes is at least two, at least two of the cuvettes are arranged in parallel, and two adjacent cuvettes are fixedly connected.

[0011] According to some embodiments of the present invention, the number of the cuvettes is at least three, and the at least three cuvettes are arranged in one row or multiple rows, and the cuvettes in each row are arranged side by side.

[0012] According to some embodiments of the present invention, when the number of the cuvettes is three, the three cuvettes are arranged in a triangle.

[0013] According to some embodiments of the present invention, a protrusion is provided on the bottom wall of the reaction chamber.

[0014] According to some embodiments of the present invention, the protrusion is provided with at least one liquid collecting inclined surface.

[0015] According to some embodiments of the present invention, the pushing device also includes: a piston assembly, which is slidingly connected to the pushing cylinder, and the cavity of the pushing cylinder is connected to the cavity of the first tube body; the piston assembly is used to generate pressure during the pushing process in the pushing cylinder, so that the sample to be tested in the capillary element flows into the reaction chamber.

[0016] According to some embodiments of the present invention, the piston assembly includes: a telescopic transmission mechanism, a piston body and a button; the button and the piston body are connected at both ends of the telescopic transmission mechanism; the button is arranged on the surface of the pushing barrel, and the telescopic transmission mechanism and the piston body are arranged in the cavity of the pushing barrel.

[0017] According to some embodiments of the present invention, the piston assembly includes: a piston body and a limit button, the piston body is slidably connected to the pushing barrel, and the limit button is arranged in the pushing barrel to limit the movement of the piston body.

[0018] According to some embodiments of the present invention, the outer diameter of the liquid storage tube is smaller than the inner diameter of the cuvette; wherein the difference between the outer diameter of the liquid storage tube and the inner diameter of the cuvette ranges from 0.001 mm to 1.00 mm.

[0019] According to some embodiments of the present invention, the difference between the outer diameter of the liquid storage tube and the inner diameter of the cuvette ranges from 0.1 mm to 0.8 mm.

[0020] According to some embodiments of the present invention, the difference between the outer diameter of the liquid storage tube and the inner diameter of the cuvette ranges from 0.3 mm to 0.5 mm.

[0021] According to some embodiments of the present invention, the sampler further includes a fool-proof positioning device acting on the cuvette and the pushing device.

[0022] According to some embodiments of the present invention, the fool-proof positioning device includes a first fool-proof unit and a second fool-proof unit, the first fool-proof unit is arranged on the cuvette, and the second fool-proof unit is arranged on the pushing device, and the first fool-proof unit and the second fool-proof unit are positioned and matched.

[0023] According to some embodiments of the present invention, the first fool-proofing unit is a positioning column, the second fool-proofing unit is provided with a positioning hole, and the positioning column is passed through the positioning hole.

[0024] According to some embodiments of the present invention, the first fool-proofing unit is a first magnetic block, and the second fool-proofing unit is a second magnetic block arranged corresponding to the first magnetic block.

[0025] According to some embodiments of the present invention, the first fool-proofing unit is a positioning protrusion, and the second fool-proofing unit is a positioning groove, and the positioning protrusion is engaged with the positioning groove.

[0026] According to some embodiments of the present invention, the shape of the projection of the positioning post in the vertical direction is the same as the shape of the positioning hole, and the area of the projection of the positioning post in the vertical direction is smaller than the area of the positioning hole.

[0027] According to some embodiments of the present invention, a limiter is provided on the inner ring of the liquid storage tube, and the limiter is used to limit the moving distance of the pushing device in the cuvette.

[0028] According to some embodiments of the present invention, a pressing portion is provided around the upper opening of the pushing barrel.

[0029] According to some embodiments of the present invention, a reinforcing rib is provided between the pressing portion and the pushing barrel.

[0030] According to some embodiments of the present invention, an interference ring is provided on the outer surface of the pushing barrel.

[0031] According to some embodiments of the present invention, the sampler also includes: a magnetic device and magnetic beads, the magnetic device is arranged on the outside of the bottom cavity wall of the reaction chamber, the magnetic beads are placed in the first detection reagent contained in the reaction chamber, and there is a magnetic force between the magnetic device and the magnetic beads.

[0032] According to some embodiments of the present invention, an outer wall of the sampling head of the capillary element is provided with a surface coating.

[0033] According to some embodiments of the present invention, the surface coating is a polytetrafluoroethylene coating, or a polyethylene glycol coating, or a dual-ion coating.

[0034] According to some embodiments of the present invention, the dual-ion coating is a cationic-anionic coating or a zwitterionic coating.

[0035] A detection device according to an embodiment of the second aspect of the present invention includes a sampler as described in any one of the embodiments of the first aspect.

[0036] Additional aspects and advantages of the present invention will be given in part in the following description and will become apparent from the following description or learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0038] Figure 1 This is a schematic diagram of the structure of a sampler provided by an embodiment of the present invention;

[0039] Figure 2 This is a schematic structural diagram of two cuvettes arranged side by side provided by an embodiment of the present invention;

[0040] Figure 3 This is a schematic structural diagram of three cuvettes arranged in parallel provided by one embodiment of the utility model;

[0041] Figure 4 This is a schematic diagram of the specific structure of a capillary element provided by one embodiment of the present utility model;

[0042] Figure 5 This is a schematic structural diagram of a liquid storage tube provided by one embodiment of the present application;

[0043] Figure 6 This is a schematic diagram of a fool-proof positioning device for positioning and connecting a cuvette and a pushing device provided in one embodiment of the present application;

[0044] Figure 7 This is a schematic structural diagram of an arrangement of three cuvettes provided in another embodiment of the present application;

[0045] Figure numerals: sampler 100, cuvette 110, accommodating chamber 111, reaction chamber 112, liquid storage tube 120, limiter 121, capillary element 130, first tube body 131, second tube body 132 and sampling head 133, pressure regulating port 134, pushing device 140, pushing cylinder 141, pressing part 1411, pushing cavity 1412, fool-proof positioning device 150, first fool-proof unit 151, second fool-proof unit 152, protrusion 113, liquid collecting inclined surface 114. DETAILED DESCRIPTION

[0046] The following describes embodiments of the present invention in detail. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended only to explain the present invention and are not to be construed as limiting the present invention.

[0047] In the description of the present invention, it should be understood that descriptions involving orientations, such as up, down, front, back, left, right, etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, they cannot be understood as limitations on the present invention.

[0048] In the description of this utility model, "several" means one or more, "many" means more than two, "greater than," "less than," and "exceed" are understood to exclude the number itself, while "above," "below," and "within" are understood to include the number itself. The use of "first" and "second" in the description is solely for the purpose of distinguishing technical features and is not to be construed as indicating or implying relative importance, implicitly specifying the number of the indicated technical features, or implicitly specifying the order of the indicated technical features.

[0049] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, and connecting should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.

[0050] Reference Figures 1 to 4 The first embodiment of the present invention provides a sampler 100, comprising: a plurality of cuvettes 110, each provided with a communicating accommodating chamber 111 and a reaction chamber 112, wherein the side wall of the reaction chamber 112 is light-transmissive; the reaction chamber 112 is used to hold a first detection reagent; a liquid storage tube 120, detachably connected to the accommodating chamber 111, the opening of the liquid storage tube 120 being provided with a sealing film, and the liquid storage tube 120 being used to hold a second detection reagent; a capillary element 130, comprising a first tube body 131, a second tube body 132 And the sampling head 133, the first tube body 131, the second tube body 132 and the sampling head 133 are connected and communicated in sequence, and the second tube body 132 is provided with a pressure regulating port 134; the sampling head 133 is used to collect the sample to be tested; the pushing device 140 includes a pushing cylinder 141; the pushing cylinder 141 is detachably connected to the first tube body 131; it is used to push the capillary element 130 to move toward the liquid storage tube 120, so that the sampling head 133 pierces the sealing membrane, allowing the second detection reagent and the sample to be tested to flow into the reaction chamber 112.

[0051] According to the sampler 100 of the embodiment of the present invention, a plurality of cuvettes 110 are provided in the sampler 100, each cuvette 110 is provided with a communicating accommodating chamber 111 and a reaction chamber 112, the reaction chamber 112 contains a first detection reagent, and the side wall of the reaction chamber 112 is light-transmissive so as to facilitate subsequent detection and analysis by using the detection device 200 to capture the light signal before and after the chemical reaction; the accommodating chamber 111 is detachably connected to the liquid storage tube 120, and the opening of the liquid storage tube 120 is provided with a sealing film so as to contain and store the second detection reagent; the pushing cylinder 141 in the pushing device 140 is connected to the capillary element The first tube body 131 of the capillary element 130 is detachably connected, and the sampling head 133 of the capillary element 130 can collect the sample to be tested by capillary action, eliminating the complicated sampling operation steps and reducing the difficulty of sampling. After the collection is completed, the pushing cylinder 141 is pushed to push the capillary element 130 toward the liquid storage tube 120, so that the sampling head 133 pierces the sealing membrane of the liquid storage tube 120, allowing the second detection reagent and the sample to be tested to flow into the reaction chamber 112 to produce a chemical reaction with the first detection reagent. The detection is achieved without the need for complicated detection operations, thereby ensuring the accuracy and reliability of home self-testing. Therefore, the sampler 100 provided by the present invention can provide simpler sampling and detection operations to ensure the accuracy and reliability of home self-testing.

[0052] Furthermore, the cuvette 110 provided in the embodiment of the present application is described.

[0053] According to some embodiments of the present invention, there are at least two cuvettes 110, which are arranged side by side, and two adjacent cuvettes 110 are fixedly connected. It is understood that one cuvette 110 can complete the detection of one group of samples, and by providing multiple cuvettes 110, multiple groups of samples can be tested simultaneously, thereby improving detection efficiency.

[0054] Specifically, if Figure 2As shown, taking two cuvettes 110 side by side as an example, a fixing element is provided between the upper ends of the cuvettes 110, and the fixing element fixes the two cuvettes 110 together to prevent the two cuvettes 110 from tilting in the front-back direction. When two adjacent cuvettes 110 are connected in a side-by-side manner using a fixing element, then the two adjacent cuvettes 110 share a fixing element. It is understandable that when there are more (N) cuvettes 110 arranged side by side, then N-1 fixing elements can be used, that is, one fixing element is shared between every two adjacent cuvettes 110. In this way, the use efficiency of the fixing element is maximized, and it is ensured that each cuvette 110 has been well supported and fixed. When the fixing element is arranged on the upper end of the cuvette 110, it is possible to prevent the cuvette 110 from tilting in the front-back direction. At the same time, the fixing element can ensure that the cuvette 110 remains stable during operation, reducing the test error caused by tilting. The fixing element here can be a separate element that fixes different cuvettes 110 together, or it can be an integrally formed element made of the same material during the process of manufacturing the cuvettes 110 .

[0055] According to some embodiments of the present invention, the number of cuvettes is at least three, and the at least three cuvettes are arranged in one or more rows, and the cuvettes in each row are arranged side by side.

[0056] like Figure 3 As shown, in some embodiments, three cuvettes 110 may be fixedly connected in parallel and in a row.

[0057] According to some embodiments of the present invention, when there are three cuvettes, the three cuvettes are arranged in a triangle, and each side of the triangle in the triangular arrangement can be regarded as consisting of a row of cuvettes.

[0058] Specifically, if Figure 7 As shown, in some embodiments, three cuvettes 110 are arranged in a triangle, and two adjacent cuvettes 110 are fixedly connected in parallel and side by side.

[0059] It should be noted that, among the multiple cuvettes 110 arranged in parallel, the fixed connection between each two adjacent cuvettes 110 can be achieved by: connection via a fixing element, welding, snap connection, hot melt connection, bolt connection, adhesive connection via an adhesive, riveting, etc. Therefore, this application does not specifically limit the method of fixed connection between two adjacent cuvettes 110. When a fixed connection method is adopted, there is a connecting portion between two adjacent cuvettes 110. This application does not specifically limit the location of the connection portion, as long as the cuvettes 110 are arranged in parallel.

[0060] It is understandable that the sampler 100 includes a plurality of cuvettes 110 , and the number of the liquid storage tubes 120 and the number of the capillary elements 130 in the sampler 100 is the same as the number of the cuvettes 110 to ensure that the sampler 100 can be used normally.

[0061] It is understandable that the number of capillary elements 130 used can be determined based on actual sampling needs. After sampling, a matching number of cuvettes 110 can be further selected for testing. Users can flexibly perform self-testing at home according to testing needs.

[0062] In some embodiments, the upper portion of the cuvette 110 includes a cylindrical cavity 111, and the side walls of the reaction chamber 112 are made of a transparent material, forming a light-transmitting region. This light-transmitting region allows light to pass through and illuminate the reaction chamber 112, thereby triggering a biochemical reaction in the reaction chamber 112 or observing the color change of a reagent.

[0063] It should be noted that the cuvette 110 provided in the embodiment of the present application is provided with a first detection reagent and a second detection reagent, wherein the first detection reagent is stored in the reaction chamber 112 of the cuvette 110, and the second detection reagent is stored in the liquid storage tube 120. The first detection reagent and the second detection reagent are placed separately for easy storage. In the cuvette 110, the pushing device 140 is used to push the capillary element 130 to move toward the liquid storage tube 120. After moving a certain distance, the sampling head 133 will pierce the sealing film, and under the action of gravity, the second detection reagent automatically flows into the reaction chamber 112. At the same time, the sample to be tested in the sampling head 133 will also flow into the reaction chamber 112. In the reaction chamber 112, the sample to be tested, the first detection reagent and the second detection reagent produce a chemical reaction to complete the detection. An external light source is injected into the reaction chamber 112 through the light-transmitting area so that the detection device 200 can capture the changes in the light signal after the chemical reaction to analyze and quantify the test results.

[0064] It is understandable that the expression of the first detection reagent and the second detection reagent is mainly to distinguish two detection reagents that are different in composition or function. The two detection reagents may be different types of reagents or reagents with different detection targets. For example, the first detection reagent may be an antibody for detecting a specific protein, and the second detection reagent may be an antibody for detecting another specific protein. In some cases, the first detection reagent and the second detection reagent may be the same reagent but are used for different purposes or detection stages. For example, the first detection reagent may be used in the preliminary screening stage, and the second detection reagent may be used for subsequent verification tests. Therefore, the present application does not impose specific restrictions on the specific composition of the first detection reagent and the second detection reagent used, and can be selected and configured according to actual detection needs.

[0065] like Figure 2As shown, according to some embodiments of the present invention, a raised portion 113 is provided on the bottom wall of the reaction chamber 112. By providing the raised portion 113, the liquid level of the reaction chamber 112 is changed, so that the reaction liquid can be relatively evenly distributed in the illumination area, so as to facilitate the collection of effective light signals and make the detection results more reliable and accurate.

[0066] Specifically, in the cuvette 110, a raised portion 113 can be provided on the entire or partial bottom wall of the reaction chamber 112. The raised portion 113 can be hemispherical, semi-arc-shaped, trapezoidal, or the like. The size and position of the raised portion can be set according to the size of the cuvette 110 and the sample volume. The height, diameter, and position of the raised portion should ensure that the sample can be effectively distributed in the illuminated area. Therefore, the embodiment of the present application does not impose any specific restrictions on the shape, size, or specific location of the raised portion 113 on the bottom wall, as long as it can change the liquid level distribution in the reaction chamber 112 so that the liquid is relatively evenly distributed in the illuminated area.

[0067] like Figure 2 As shown, according to some embodiments of the present invention, the protrusion 113 is provided with at least one liquid collecting inclined surface 114. By providing the liquid collecting inclined surface 114, the liquid in the reaction chamber 112 is further gathered to increase the liquid level.

[0068] It is understandable that since the sample to be tested drawn by a single capillary is usually 5 to 20 μL, it is understandable that the volume of the sample to be tested drawn by a single capillary is not limited, for example, it can be 1 μL, 2 μL, 5 μL, 10 μL, 20 μL, etc. Due to the small sample size, if the bottom of the reaction chamber 112 of the cuvette 110 is horizontal, it may cause the mixed solution after the reaction of the sample to be tested, the first detection reagent, and the second detection reagent to be near the bottom of the cuvette 110, and there is a situation where the light source signal cannot be injected into the mixed solution, and some chemical reactions that require light signals for stimulation will not be realized; and it is also difficult to irradiate the mixed solution after the reaction is completed to collect the changing light signals. The present application can make the trace sample as distributed as much as possible in the illumination area in the vertical direction of the cuvette 110 by providing the raised portion 113 and the liquid collecting inclined surface 114, so that the subsequent detection based on the light signal is more accurate.

[0069] The accommodating cavity 111 of the cuvette 110 is used to accommodate the liquid storage tube 120. Further, the liquid storage tube 120 provided in the embodiment of the present application is described.

[0070] like Figure 5 As shown, Figure 51 is a schematic diagram of the structure of a liquid storage tube 120 provided in one embodiment of the present application. According to some embodiments of the present invention, the inner ring of the liquid storage tube 120 is provided with a limiter 121, which is used to limit the movement distance of the pushing device 140 in the cuvette 110.

[0071] It is understandable that by providing a limit member 121 on the inner ring of the liquid storage tube 120 , the pushing cylinder 141 stops moving when it is pushed to the position where the limit member 121 is located, thereby improving the operating accuracy and reliability of the equipment.

[0072] It should be noted that the limiter 121 is a structure fixed to the inner ring of the liquid storage tube 120 or a structure integrally formed with the liquid storage tube 120, and is used to control the movement range of the push barrel 141. The limiter 121 acts as a physical limiter or trigger, interacting with the push barrel 141 to achieve precise displacement control. The introduction of the limiter 121 as a key component is to ensure that the push barrel 141 stops moving when it moves to a specific position, thereby controlling the precise output and input of liquid. This design can improve the operational accuracy and reliability of the device.

[0073] Specifically, the stopper 121 can be a protruding annular protrusion, an independent bump, or any other shape; therefore, this application does not impose any specific restrictions on the shape of the stopper 121 of the liquid storage tube 120. In this case, no additional stopper is required on the push barrel 141, and the stopper 121 alone can also achieve the limiting function.

[0074] In some embodiments, the outer wall of the push barrel 141 is further provided with a limiting portion (such as a cam, hook, or groove) that matches the limiting member 121. The limiting member 121 cooperates with the limiting portion to achieve a limiting function. When the user pushes the push barrel 141, the push barrel 141 moves forward or backward along the liquid storage tube 120.

[0075] The limiting portion (which may be a small arm or protrusion) on the push barrel 141 can be a trigger that can activate the displacement stop mechanism when it contacts the limiting member 121. Specifically, it can be through mechanical blocking (such as inserting a locking pin) or electronic signal control (such as a sensor configured on the push barrel 141 detects the limiting member 121 and sends a stop signal to make the user stop moving the push barrel 141).

[0076] According to some embodiments of the present invention, the outer diameter of the liquid storage tube 120 is smaller than the inner diameter of the cuvette 110; wherein, the difference between the outer diameter of the liquid storage tube and the inner diameter of the cuvette is in the range of 0.001 mm to 1.00 mm; or, the difference between the outer diameter of the liquid storage tube and the inner diameter of the cuvette is in the range of 0.1 mm to 0.8 mm; or, the difference between the outer diameter of the liquid storage tube and the inner diameter of the cuvette is in the range of 0.3 mm to 0.5 mm.

[0077] It is understood that, because the liquid storage tube 120 needs to be embedded in the receiving chamber 111 of the cuvette 110, the diameter of the liquid storage tube 120 is designed so that the outer diameter of the liquid storage tube 120 is slightly smaller than the inner diameter of the receiving chamber 111 of the cuvette 110. For example, the difference between the outer diameter of the liquid storage tube 120 and the inner diameter of the receiving chamber 111 of the cuvette 110 is in the range of 0.001 mm to 1.00 mm, or in the range of 0.1 mm to 0.8 mm, or in the range of 0.3 mm to 0.5 mm. This difference ensures that the liquid storage tube 120 can be relatively easily embedded in the cuvette 110 while maintaining sufficient sealing, thereby sealing the first detection reagent in the reaction chamber 112 of the cuvette 110 and preventing it from deteriorating in the air.

[0078] The opening of the liquid reservoir 120 is provided with a sealing membrane. Specifically, the top opening of the liquid reservoir 120 is sealed with a tearable membrane, and the bottom opening is sealed with a puncture-resistant membrane. Both the tearable membrane and the puncture-resistant membrane are the sealing membranes mentioned in the embodiments of this application. During use, the user can directly tear off the tearable membrane of the liquid reservoir 120, then insert the capillary element 130 into the liquid reservoir 120. Under the push of the push cylinder 141, the puncture-resistant membrane is pierced, allowing the sample to be tested in the capillary element 130 and the second detection reagent in the liquid reservoir 120 to flow into the reaction chamber 112 of the cuvette 110, and undergo a biochemical reaction with the first detection reagent in the reaction chamber 112.

[0079] Furthermore, the capillary element 130 provided in the embodiment of the present application is described.

[0080] It is understood that capillary element 130 exhibits capillary action, which refers to the phenomenon of liquid rising or falling in a narrow tube or slit. During sampling, the sampling head 133 of capillary element 130 is immersed in the sample to be tested. Under capillary action, the sample to be tested rises along the sampling head 133, completing the sampling process without external force, thus simplifying the sampling operation.

[0081] It is understood that the capillary element 130 and the pushing device 140 are detachably connected. Therefore, after sampling is completed, during the transfer process of the sample to be tested, the capillary element 130 and the pushing device 140 are physically connected. The pushing device 140 is then used to insert the capillary element 130 into the cuvette 110, and the pushing cylinder 141 is pushed to push the capillary element 130 toward the liquid reservoir 120, so that the sampling head 133 pierces the sealing membrane of the liquid reservoir 120, allowing the second detection reagent and the sample to be tested to flow into the reaction chamber 112 and react with the first detection reagent, completing the sample test. This eliminates the traditional and more complicated sample transfer steps and reduces the potential contamination or loss of the sample to be tested during the transfer process.

[0082] It should be noted that the push barrel 141 and the capillary element 130 are fixed in a detachable manner, so that the capillary element 130 can be easily installed or removed from the push barrel 141, thereby achieving flexible replacement. In the present application, the capillary element 130 is fixed to the push barrel 141 in a detachable manner, but the user does not need to remove it by himself. However, in some special cases, for example, the user accidentally wets the sampling head 133 but does not absorb the liquid. This may result in the inability to perform the operation when absorbing the liquid again or the volume of the absorbed liquid sample does not meet the requirements. At this time, the user can replace the spare capillary element 130 by himself to achieve better sampling and detection effects.

[0083] In some embodiments, a first interface is provided at one end of the pusher barrel 141, and a second interface matching the first interface is provided on the first tube body 131 of the capillary element 130. The first interface and the second interface are detachably connected, thereby enabling a quick and secure connection to the capillary element 130. A threaded interface allows for a secure connection by rotation, while a snap-on interface allows for connection and removal by a simple snap-in and pull-out action.

[0084] To connect the pusher barrel 141 and capillary element 130, the user can connect them using a pre-designed interface. For example, with a threaded connection, the user inserts the pusher barrel 141 into the interface of the capillary element 130 and rotates to tighten the threads until they are securely fastened. With a snap-on connection, the user aligns the snaps and applies pressure to lock them into place, completing the connection. To disassemble, the user can quickly remove the pusher barrel 141 by rotating the threads in the opposite direction or pressing the snap release mechanism.

[0085] It is understood that the detachable connection method can be a threaded connection, a snap-on connection, a magnetic connection, or other mechanical connection methods that facilitate quick disassembly and installation. Therefore, this application does not impose specific restrictions on the implementation method of the detachable connection.

[0086] It should be noted that the samples collected by the capillary element 130 include: in vitro blood, urine, saliva, feces, cerebrospinal fluid, body fluid, respiratory secretions, cells or tissue samples. This application does not impose any specific restrictions on the type of samples to be tested.

[0087] Furthermore, the pushing device 140 provided in the embodiment of the present application is described.

[0088] like Figure 6As shown, the pusher barrel 141 is provided with a pusher cavity 1412, which is used to accommodate a piston assembly. According to some embodiments of the present invention, the pusher device 140 further includes a piston assembly, which is slidably connected to the pusher barrel 141, and the cavity of the pusher barrel 141 is connected to the cavity of the first tube 131. The piston assembly is used to generate pressure during the process of pushing the pusher barrel 141, so that the sample to be tested in the capillary element 130 flows into the reaction chamber 112.

[0089] According to some embodiments of the present invention, the piston assembly includes: a telescopic transmission mechanism, a piston body, and a button; the button and the piston body are connected at both ends of the telescopic transmission mechanism; the button is disposed on the surface of a pusher barrel 141, and the telescopic transmission mechanism and the piston body are disposed within the cavity of the pusher barrel 141. It is understood that the pusher barrel 141 has a cavity, and the number of pusher barrels 141 is the same as the number of capillary elements 130. Each cavity is provided with a piston assembly, which is moved by pressing the button on the pusher barrel 141, thereby puncturing the puncture-prone membrane at the bottom end of the liquid storage tube 120.

[0090] The user activates the telescopic transmission mechanism by pressing a button on the push cylinder 141. The telescopic transmission mechanism drives the piston body to move in the cavity, generating pressure. Under the action of pressure, the sample to be tested in the capillary element 130 flows into the liquid storage tube 120. As the push cylinder 141 moves, the sealing membrane at the bottom of the liquid storage tube 120 is punctured. The second detection reagent and the liquid sample to be tested in the liquid storage tube 120 flow into the reaction chamber 112 of the cuvette 110 and mix with the first detection reagent.

[0091] The integrated cavity design of the push cylinder 141 is conducive to providing multiple independent liquid transfer channels when multiple push cylinders 141 are used, and also realizes precise control of the sample to be tested through the internal piston mechanism.

[0092] According to some embodiments of the present invention, the piston assembly includes a piston body and a limit button. The piston body is slidably connected to the push barrel 141. The limit button is provided in the push barrel 141 to limit the movement of the piston body. The limit button is provided to prevent the piston body from being accidentally triggered.

[0093] In some embodiments, the pushers or buttons of the pusher barrel 141 are configured with different colors, each corresponding to a specific color of the liquid reservoir 120. This color correspondence allows the user to easily identify and match the pusher barrel 141 with the liquid reservoir 120, ensuring correct insertion and operation. In actual use, the user can accurately insert the pusher barrel 141 into the corresponding liquid reservoir 120 simply by identifying and matching the colors, reducing the possibility of misoperation caused by similar colors or a large number of liquid reservoirs 120.

[0094] like Figure 6 As shown, according to some embodiments of the present invention, a pressing portion 1411 is provided around the upper opening of the pushing cylinder 141 to facilitate pushing the pushing cylinder 141 or pushing the piston.

[0095] According to some embodiments of the present invention, reinforcing ribs are provided between the pressing portion 1411 and the pusher barrel 141. These ribs are typically located at the point of greatest force, i.e., the pressing area, to enhance the overall strength and rigidity of the pusher barrel 141. During the movement of the pusher barrel 141, the pressing area is often the point of greatest force. The provision of these ribs enhances the rigidity and strength of the pusher barrel 141, enabling a single press to synchronize the movement of multiple pistons, allowing the liquid in each group of capillary elements 130 to be simultaneously expelled and preventing deformation or damage to the pusher barrel 141 during operation.

[0096] According to some embodiments of the present invention, an interference ring is provided on the outer surface of the pusher barrel 141. It is understood that interference rings are typically installed at key locations on the pusher barrel 141, such as around the piston or at the entrance to the liquid channel, to provide additional sealing or support. The tight fit of the interference ring forms an effective sealing interface, preventing leakage of liquid or gas.

[0097] like Figure 6 As shown, according to some embodiments of the present invention, the sampler 100 further includes a foolproof positioning device 150 acting on the cuvette 110 and the pushing device 140. By providing the foolproof positioning device 150 to achieve physical positioning, the correct assembly of the cuvette 110 and the pushing device 140 is ensured, thereby ensuring the correct use of the sampler 100 and reducing problems caused by misoperation.

[0098] According to some embodiments of the present invention, the foolproof positioning device 150 includes a first foolproof unit 151 and a second foolproof unit 152. The first foolproof unit 151 is provided on the cuvette 110, and the second foolproof unit 152 is provided on the pushing device 140. The first foolproof unit 151 and the second foolproof unit 152 are positioned and matched. The provision of the first foolproof unit 151 and the second foolproof unit 152 guides the user in assembling the cuvette 110 and the pushing device 140, thereby ensuring the correct assembly of the cuvette 110 and the pushing device 140.

[0099] According to some embodiments of the present invention, the first foolproofing unit 151 is a positioning post, and the second foolproofing unit 152 is provided with a positioning hole, through which the positioning post is inserted. The positioning elements and positioning holes ensure that during installation or operation, the lower swing element can only pass through the through hole when the pusher barrel 141 is properly aligned with the cuvette 110. This structural design effectively prevents possible installation or operation errors by the user. The foolproofing design ensures correct use of the device through physical constraints, reducing problems caused by incorrect operation.

[0100] According to some embodiments of the present invention, the shape of the projection of the positioning post in the vertical direction is the same as the shape of the positioning hole, and the area of the projection of the positioning post in the vertical direction is smaller than the area of the positioning hole.

[0101] It can be understood that the area of the projection of the positioning column in the vertical direction can be smaller than the area of the positioning hole so that the positioning column can be passed through the positioning hole. Therefore, this application does not impose specific restrictions on the area of the projection of the positioning column in the vertical direction and the area of the positioning hole, and can be set according to actual conditions.

[0102] It is understandable that the positioning post can be designed in a variety of shapes and sizes to adapt to different application requirements and fool-proofing purposes. For example, the positioning post is cylindrical, and the diameter and length can be designed according to the size of the push cylinder 141 and the size of the positioning hole of the cuvette 110. A circular through hole is configured on the side of the cuvette 110 to match the cylindrical positioning post. The diameter of the through hole should match the diameter of the positioning post to ensure that the positioning post can pass through smoothly. In addition, the positioning post can be a square cylinder, which has clear edges and angles and can provide a clearer positioning and fool-proofing effect. The through hole corresponding to the square positioning post can be a square through hole. In addition, raised marks or symbols can be designed on the positioning post to further enhance the fool-proofing effect. The user can identify the correct installation position by sight or touch. Therefore, the present application does not impose specific restrictions on the shape of the positioning post.

[0103] According to some embodiments of the present invention, the first fool-proofing unit 151 is a first magnetic block, and the second fool-proofing unit 152 is a second magnetic block arranged corresponding to the first magnetic block.

[0104] Specifically, a first magnetic block and a second magnetic block with opposite magnetic properties can be provided so that when assembling the cuvette 110 and the pushing device 140 , the mutual attraction between the first magnetic block and the second magnetic block can guide the user to correctly assemble the cuvette 110 and the pushing device 140 .

[0105] Specifically, a first magnetic block and a second magnetic block with the same magnetic properties can be provided so that when assembling the cuvette 110 and the pushing device 140, the mutual repulsion of the first magnetic block and the second magnetic block can prompt the user that the cuvette 110 and the pushing device 140 are assembled incorrectly and the assembly direction needs to be changed.

[0106] It is understandable that the positions of the first magnetic block and the second magnetic block can be selected according to needs. Therefore, the present application does not impose specific restrictions on the positions of the first magnetic block and the second magnetic block, as well as the specific magnetism used, as long as the first magnetic block and the second magnetic block can cooperate with each other to achieve positioning and matching.

[0107] According to some embodiments of the present invention, the first fool-proofing unit 151 is a positioning protrusion, and the second fool-proofing unit 152 is a positioning groove, and the positioning protrusion is engaged with the positioning groove.

[0108] Specifically, the positioning protrusion can be shaped like a cube, and the corresponding positioning groove can be shaped like a cube to achieve positioning matching. Alternatively, the positioning protrusion can be shaped like a tooth, and the corresponding positioning groove can be shaped like a tooth to achieve positioning matching. Therefore, this application does not impose any specific restrictions on the shapes of the positioning protrusion and the positioning groove.

[0109] like Figure 7 As shown, according to some embodiments of the present invention, the sampler 100 further includes a magnetic device 240 and magnetic beads. The magnetic device 240 is disposed on the outer side of the bottom wall of the reaction chamber 112. The magnetic beads are placed in the first detection reagent contained in the reaction chamber 112. A magnetic force is generated between the magnetic device 240 and the magnetic beads. The magnetic beads can rotate under the magnetic force of the magnetic device 240, stirring the liquid mixture in the cuvette 110 to ensure sufficient reaction.

[0110] It is understandable that one or more magnetic beads can be placed in the reaction chamber 112, and these magnetic beads can rotate or move under the action of the magnetic device 240. When the magnetic device 240 is started, the magnetic field generated by the magnetic device 240 interacts with the magnetic beads in the cuvette 110, driving the magnetic beads to rotate or move in the reaction chamber 112, thereby generating a stirring effect in the liquid, so that the mixture in the cuvette 110 can be evenly mixed. This is suitable for detection processes that require maintaining a uniform distribution of samples, such as chemical reactions, enzyme reactions, or particle suspension detection. Therefore, the present application does not impose any specific restrictions on the number of magnetic beads provided.

[0111] It is understandable that if Figure 7As shown, the magnetic device 240 can be disposed on the outside of the bottom cavity wall, or can be disposed at the bottom of the base of the cuvette 110 of the detection device 200 , thereby cooperating with the magnetic beads in the reaction chamber 112 of the cuvette 110 to stir the liquid mixture in the reaction chamber 112 .

[0112] The detection device 200 is provided with a magnetic device 240 at the bottom of the base of the cuvette 110. The magnetic device 240 works in conjunction with the magnetic beads at the bottom of the cuvette 110 to stir the mixture in the cuvette 110. By providing the magnetic device 240 at the bottom of the base of the cuvette 110, the magnetic device 240 interacts with the magnetic beads in the cuvette 110, thereby achieving effective stirring of the mixture in the cuvette 110.

[0113] Specifically, the magnetic device 240 may be an electromagnet or a permanent magnet, configured to generate sufficient magnetic force to interact with the magnetic beads in the cuvette 110 .

[0114] According to some embodiments of the present invention, the outer wall of the sampling head of the capillary element is provided with a surface coating. This coating can reduce nonspecific adsorption between the sample and the outer wall of the capillary, which is crucial for maintaining sample integrity and concentration. Reducing adsorption can prevent sample loss and contamination, particularly when processing biomolecules such as proteins and nucleic acids. However, due to the small sample volume, if a large amount of sample is adsorbed onto the outer wall, the sample volume drawn by the sampling head will be inaccurate.

[0115] According to some embodiments of the present invention, the surface coating is preferably a polytetrafluoroethylene (PTFE) coating or a polyethylene glycol (PEG) coating. PTFE coatings have extremely low surface energy, providing excellent anti-stick properties. Furthermore, they are chemically inert, adaptable to various sample types, and non-reactive. PEG coatings, on the other hand, can form a highly hydrated surface, effectively reducing nonspecific adsorption of biomolecules such as proteins. PEG coatings also have excellent biocompatibility, making them particularly suitable for the collection and analysis of biological samples.

[0116] According to some embodiments of the present invention, the surface coating may preferably adopt a dual-ion coating structure. This innovative coating design is intended to significantly reduce the adsorption of the sample to be tested on the outer wall of the capillary, thereby ensuring that the sample volume absorbed from the inside of the capillary is more accurate. Dual-ion coatings mainly include two types: the first is a cationic-anionic coating. This type of coating is formed by electrostatic interaction of polyelectrolytes with opposite charges. Typical examples include polyethyleneimine (PEI, cationic)-polyacrylic acid (PAA, anionic) composite coatings, and chitosan (cationic)-sodium alginate (anionic) composite coatings. This type of coating can form a layer of uniform charge distribution on the outer surface of the capillary, effectively reducing the nonspecific adsorption of charged particles in the sample to the outer wall of the capillary. The second type is a zwitterionic coating, such as a polylysine-polyglutamic acid copolymer coating and a polydopamine-polyethyleneimine composite coating. This type of coating contains both cationic and anionic groups in its molecular structure, and can form a layer of electrically neutral hydration layer on the surface of the capillary. This hydration layer significantly reduces the interaction between sample components and the capillary wall, further reducing nonspecific adsorption. The use of these dual-ion coatings improves the accuracy of sample volume aspirated by the capillary. Because adsorption to the outer wall is minimized, the capillary can more precisely aspirate the desired volume of sample.

[0117] In some embodiments, a detection device according to the second aspect of the present invention includes a sampler according to any one of the first aspect embodiments. The detection device provided according to the second aspect of the present invention can provide simpler sampling and detection operations by utilizing the sampler to ensure the accuracy and reliability of self-detection at home.

[0118] It should be noted that this operation method does not involve living human or animal bodies, nor is it directly intended to obtain disease diagnosis results or health status. It is only a method of processing or testing tissues, body fluids or excretions that have been separated from the human or animal body to obtain information as an intermediate result.

[0119] The embodiments of the present invention are described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Various changes can be made within the scope of knowledge possessed by ordinary technicians in the technical field without departing from the purpose of the present invention.

Claims

1. A sampler, characterized in that: include: A plurality of cuvettes are provided with a communicating receiving cavity and a reaction cavity, wherein the side walls of the reaction cavity are light-transmissive; The reaction chamber is used to hold a first detection reagent; a liquid storage tube, detachably connected to the accommodating cavity, wherein an opening of the liquid storage tube is provided with a sealing film, and the liquid storage tube is used to hold a second detection reagent; The capillary element comprises a first tube body, a second tube body and a sampling head, wherein the first tube body, the second tube body and the sampling head are sequentially connected and communicated, and the second tube body is provided with a pressure regulating port; the sampling head is used to collect a sample to be tested; The pushing device includes a pushing cylinder; the pushing cylinder is detachably connected to the first tube body; and is used to push the capillary element toward the liquid storage tube so that the sampling head pierces the sealing membrane, allowing the second detection reagent and the sample to be tested to flow into the reaction chamber.

2. The sampler according to claim 1, characterized in that The number of the cuvettes is at least two, at least two of the cuvettes are arranged in parallel, and two adjacent cuvettes are fixedly connected.

3. The sampler according to claim 2, characterized in that The number of the cuvettes is at least three, and the at least three cuvettes are arranged in one row or multiple rows, and the cuvettes in each row are arranged side by side.

4. The sampler according to claim 2, characterized in that When the number of the cuvettes is three, the three cuvettes are arranged in a triangle.

5. The sampler according to claim 1, characterized in that The bottom wall of the reaction chamber is provided with a protrusion.

6. The sampler according to claim 5, characterized in that The protrusion is provided with at least one liquid collecting inclined surface.

7. The sampler according to claim 1, characterized in that The pushing device also includes: a piston assembly, which is slidably connected to the pushing cylinder, and the cavity of the pushing cylinder is connected to the cavity of the first tube body; the piston assembly is used to generate pressure during the pushing process in the pushing cylinder, so that the sample to be tested in the capillary element flows into the reaction chamber.

8. The sampler according to claim 7, characterized in that The piston assembly includes: a telescopic transmission mechanism, a piston body and a button; the button and the piston body are connected to both ends of the telescopic transmission mechanism; the button is arranged on the surface of the pushing barrel, and the telescopic transmission mechanism and the piston body are arranged in the cavity of the pushing barrel.

9. The sampler according to claim 7, characterized in that The piston assembly includes a piston body and a limit button. The piston body is slidably connected to the pushing barrel. The limit button is arranged in the pushing barrel to limit the movement of the piston body.

10. The sampler according to claim 1, characterized in that The outer diameter of the liquid storage tube is smaller than the inner diameter of the cuvette; wherein the difference between the outer diameter of the liquid storage tube and the inner diameter of the cuvette is in the range of 0.001 mm to 1.00 mm.

11. The sampler according to claim 10, characterized in that The difference between the outer diameter of the liquid storage tube and the inner diameter of the cuvette is in the range of 0.1 mm to 0.8 mm.

12. The sampler according to claim 11, characterized in that The difference between the outer diameter of the liquid storage tube and the inner diameter of the cuvette is in the range of 0.3 mm to 0.5 mm.

13. The sampler according to claim 1, characterized in that The sampler further comprises a fool-proof positioning device acting on the cuvette and the pushing device.

14. The sampler according to claim 13, characterized in that The foolproof positioning device includes a first foolproof unit and a second foolproof unit. The first foolproof unit is arranged on the cuvette, and the second foolproof unit is arranged on the pushing device. The first foolproof unit and the second foolproof unit are positioned and matched.

15. The sampler according to claim 14, characterized in that The first fool-proofing unit is a positioning column, the second fool-proofing unit is provided with a positioning hole, and the positioning column is passed through the positioning hole.

16. The sampler according to claim 14, characterized in that The first fool-proofing unit is a first magnetic block, and the second fool-proofing unit is a second magnetic block arranged corresponding to the first magnetic block.

17. The sampler according to claim 14, characterized in that The first fool-proofing unit is a positioning protrusion, and the second fool-proofing unit is a positioning groove, and the positioning protrusion is engaged with the positioning groove.

18. The sampler according to claim 15, characterized in that The shape of the projection of the positioning column in the vertical direction is the same as the shape of the positioning hole, and the area of the projection of the positioning column in the vertical direction is smaller than the area of the positioning hole.

19. The sampler according to claim 1, characterized in that The inner ring of the liquid storage tube is provided with a limiting member, and the limiting member is used to limit the moving distance of the pushing device in the cuvette.

20. The sampler according to claim 1, characterized in that A pressing portion is arranged around the upper opening of the pushing cylinder.

21. The sampler according to claim 20, characterized in that A reinforcing rib is provided between the pressing portion and the pushing cylinder.

22. The sampler according to claim 1, characterized in that The outer surface of the pushing cylinder is provided with an interference ring.

23. The sampler according to claim 1, characterized in that The sampler also includes: a magnetic device and magnetic beads. The magnetic device is arranged on the outside of the bottom cavity wall of the reaction chamber. The magnetic beads are placed in the first detection reagent contained in the reaction chamber. There is a magnetic force between the magnetic device and the magnetic beads.

24. The sampler according to claim 1, characterized in that The outer wall of the sampling head of the capillary element is provided with a surface coating.

25. The sampler according to claim 24, characterized in that The surface coating is a polytetrafluoroethylene coating, a polyethylene glycol coating, or a dual-ion coating.

26. The sampler according to claim 25, characterized in that The dual-ion coating is a cationic-anionic coating or a zwitterionic coating.

27. A detection device, characterized in that: Comprising a sampler as described in any one of claims 1 to 26.

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