Immobilized enzyme reaction device

What is AI technical title?
AI technical title is built by Patsnap AI team. It summarizes the technical point description of the patent document.
By designing a coaxially installed immobilized enzyme container in the immobilized enzyme reaction device to connect it with the stirring shaft, and using through holes to achieve contact between the material and the enzyme to avoid collision between the enzyme and the stirring assembly, the problem of shortening the enzyme life is solved and the long life and cost saving of the enzyme is achieved.

CN223240086UActive Publication Date: 2025-08-19ANHUI TIGER BIOTECHNOLOGY CO LTD +1

View PDF 0 Cites 0 Cited by

Patent Information

Application Number: CN202421975049.4
Authority / Receiving Office: CN · China
Patent Type: Utility models(China)
Current Assignee / Owner
Filing Date: 2024-08-13
Publication Date: 2025-08-19
Estimated Expiration: 2034-08-13

AI Technical Summary

Technical Problem

Immobilized enzymes tend to collide with the stirring assembly during the stirring process, resulting in a shortening of service life.

Method used

An immobilized enzyme reaction device is designed, wherein the immobilized enzyme container is fixed with the stirring shaft through a coaxial mounting hole, and the through holes connects the first and second storage spaces, so that the material enters the second storage space and contacts the enzyme, and the enzyme cannot leave through the through holes to avoid collision with the stirring assembly.

Benefits of technology

It extends the service life of immobilized enzymes, reduces maintenance frequency and production costs.

✦ Generated by Eureka AI based on patent content.

Smart Images

Figure CN223240086U_ABST

Patent Text Reader

Abstract

The utility model relates to the technical field of immobilized enzymes, and in particular relates to an immobilized enzyme reaction device which comprises a reaction tank, a reaction device and a control device, wherein the reaction tank comprises a first accommodating space; the stirring assembly comprises a stirring shaft and a stirring head connected to the stirring shaft, and the stirring shaft and the stirring head are both located in the first containing space; the immobilized enzyme container is positioned in the first accommodating space, the immobilized enzyme container comprises a first mounting hole, and the immobilized enzyme container is coaxially fixed with the stirring shaft through the first mounting hole; the second accommodating space is configured to accommodate an immobilized enzyme; and the second accommodating space is communicated with the first accommodating space through the through hole. According to the immobilized enzyme reaction device disclosed by the invention, the immobilized enzyme does not collide with the stirring assembly, so that the damage to the immobilized enzyme is reduced, and the service life of the immobilized enzyme is prolonged.

Need to check novelty before this filing date? Find Prior Art

Description

Technical Field

[0001] The present disclosure relates to the technical field of immobilized enzymes, and in particular to an immobilized enzyme reaction device. Background Art

[0002] Immobilized enzyme technology is a technology that binds or restricts enzymes to a certain area, allowing them to carry out catalytic reactions and be recycled and reused.

[0003] In the related art, the immobilized enzyme is placed in a reaction tank and contacts the material under the action of a stirring component to achieve a catalytic effect.

[0004] However, during the stirring process, the immobilized enzyme is prone to collide with the stirring component, causing damage to the immobilized enzyme and reducing the service life of the immobilized enzyme. Utility Model Content

[0005] The purpose of the present disclosure is to provide an immobilized enzyme reaction device to solve the problem of collision between the immobilized enzyme and the stirring component.

[0006] To achieve the above objectives, the present disclosure provides an immobilized enzyme reaction device comprising:

[0007] A reaction tank, wherein the reaction tank comprises a first accommodating space;

[0008] a stirring assembly, the stirring assembly comprising a stirring shaft and a stirring head connected to the stirring shaft, the stirring shaft and the stirring head both being located in the first accommodating space; and

[0009] an immobilized enzyme container, the immobilized enzyme container being located in the first accommodating space, and the immobilized enzyme container comprising:

[0010] a first mounting hole, through which the immobilized enzyme container is coaxially fixed to the stirring shaft;

[0011] a second accommodating space configured to accommodate an immobilized enzyme; and

[0012] A through hole connects the second accommodating space and the first accommodating space to each other.

[0013] Compared with the prior art, the advantages of the present invention include:

[0014] Under the stirring of the stirring assembly, the material in the first accommodating space can enter the second accommodating space through the through-hole of the immobilized enzyme container, thereby contacting the immobilized enzyme and completing the reaction process. During the stirring period, the immobilized enzyme cannot pass through the through-hole to leave the immobilized enzyme container, so the immobilized enzyme will not collide with the stirring assembly, reducing damage to the immobilized enzyme and extending the service life of the immobilized enzyme. Since the service life of the immobilized enzyme is extended, the maintenance frequency of the immobilized enzyme is reduced, the cost of the immobilized enzyme can be saved, and then the production cost is reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic diagram of an immobilized enzyme reaction device provided in an embodiment of the present disclosure is shown.

[0016] Figure 2 A top view of a hollow body provided by an embodiment of the present disclosure is shown.

[0017] Figure 3 A schematic diagram of the immobilized enzyme reaction device provided by an embodiment of the present disclosure is shown, wherein the lower cover is opened downward.

[0018] Figure 4 A schematic diagram of the immobilized enzyme reaction device provided by an embodiment of the present disclosure is shown, wherein the upper cover is opened upward.

[0019] Reference numerals:

[0020] 1. Reaction tank; 11. First accommodating space; 12. Inlet; 13. Outlet;

[0021] 2. Stirring assembly; 21. Stirring shaft; 22. Stirring head; 23. Driving component;

[0022] 3. Immobilized enzyme container; 31. Hollow body; 311. First mounting hole; 312. Second accommodating space; 313. Upper opening; 314. Lower opening; 315. Inner wall; 316. Outer wall; 317. Connecting plate; 32. Upper cover; 321. Second mounting hole; 33. Lower cover; 331. Third mounting hole; 34. Through hole; 35. Net;

[0023] 4. First rope;

[0024] 5. Second rope;

[0025] 6. Upper limit plate;

[0026] 7. Lower limit plate. DETAILED DESCRIPTION

[0027] The technical solutions of the present disclosure are further described below with reference to the accompanying drawings and through specific embodiments. It should be understood that the specific embodiments described herein are merely for the purpose of explaining the present disclosure and are not intended to limit the present disclosure. It should also be noted that, for ease of description, the accompanying drawings only show portions relevant to the present disclosure, not all of the present disclosure.

[0028] Some directional words are defined in the present disclosure. Unless otherwise stated, the directional words used, such as "up", "down", "left", "right", "inside" and "outside", are used for ease of understanding and therefore do not constitute a limitation on the scope of protection of the present disclosure.

[0029] In the present disclosure, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Moreover, a first feature being "above," "above," and "above" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature includes the first feature being directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0030] In the description of this disclosure, unless otherwise expressly specified or limited, the terms "connected," "connect," and "fixed" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and can refer to internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of these terms in this disclosure based on the specific circumstances.

[0031] Figure 1 Schematic diagram of the immobilized enzyme reaction device provided by the embodiment of the present disclosure is shown. Figure 1 As shown, the immobilized enzyme reaction device includes a reaction tank 1, which is generally cylindrical and has a first accommodating space 11 inside. The reaction tank 1 has an inlet 12 at the top and an outlet 13 at the bottom. The material to be reacted (usually a liquid) can enter the first accommodating space 11 through the inlet 12 and react there. The reacted material can then leave the first accommodating space 11 through the outlet 13.

[0032] like Figure 1As shown, the immobilized enzyme reaction device further includes a stirring assembly 2, which is used to stir the material in the first accommodating space 11. Specifically, the stirring assembly 2 includes a stirring shaft 21 extending into the first accommodating space 11 and one or more stirring heads 22 connected to the stirring shaft 21. The stirring shaft 21 can drive the stirring heads 22 to rotate in the first accommodating space 11, thereby stirring the material.

[0033] In order to drive the stirring shaft 21 to rotate, a driving component 23, such as a motor, can be connected to the stirring shaft 21. The driving component 23 can be installed on the top of the reaction tank 1, but is not limited thereto.

[0034] The stirring head 22 may be fixedly mounted on the stirring shaft 21 or detachably mounted on the stirring shaft 21 .

[0035] like Figure 1 As shown, the immobilized enzyme reaction device further includes an immobilized enzyme container 3 for accommodating an immobilized enzyme (not shown), and the immobilized enzyme container 3 is located in the first accommodating space 11 .

[0036] Specifically, the immobilized enzyme container 3 comprises a first mounting hole 311, a second accommodating space 312 and a through hole 34. The immobilized enzyme container 3 is coaxially fixed with the stirring shaft 21 through the first mounting hole 311. Like this, on the one hand, the immobilized enzyme container 3 can be installed in the first accommodating space 11, and on the other hand, the stirring shaft 21 can drive the immobilized enzyme container 3 to rotate in the first accommodating space 11. The second accommodating space 312 is configured to accommodate the immobilized enzyme. The through hole 34 interconnects the second accommodating space 312 with the first accommodating space 11, and the size of the through hole 34 is smaller than the size of the immobilized enzyme, so the immobilized enzyme cannot pass through the through hole 34 and leave the second accommodating space 312. However, the material in the first accommodating space 11 can pass through the through hole 34 and enter the second accommodating space 312, thereby contacting the immobilized enzyme.

[0037] Therefore, according to the immobilized enzyme reaction device disclosed herein, during the reaction, the immobilized enzyme cannot pass through the through hole 34 and leave the immobilized enzyme container 3. Therefore, the immobilized enzyme does not collide with the stirring assembly 2, thereby reducing damage to the immobilized enzyme and extending the service life of the immobilized enzyme. Since the service life of the immobilized enzyme is extended, the maintenance frequency of the immobilized enzyme is reduced, the cost of the immobilized enzyme can be saved, and thus the production cost can be reduced.

[0038] like Figure 1 As shown, the immobilized enzyme container 3 can be a hollow cylinder, the interior space of which is the second accommodation space 312. The through holes 34 can be provided on the surface of the immobilized enzyme container 3, thereby connecting the second accommodation space 312 with the first accommodation space 11. The number and arrangement of the through holes 34 are not limited.

[0039] Specifically, if Figure 1 As shown, the immobilized enzyme container 3 further comprises a hollow body 31, an upper cover 32 and a lower cover 33. The hollow body 31 comprises an upper opening 313 (see Figure 4 ), lower opening 314 (see Figure 3 ) and the first mounting hole 311, the hollow body 31 is mounted on the stirring shaft 21 through the first mounting hole 311, and the area between the upper opening 313 and the lower opening 314 is the second accommodating space 312. The upper cover 32 covers the upper opening 313 and includes a second mounting hole 321 for allowing the stirring shaft 21 to pass through. The lower cover 33 covers the lower opening 314 and includes a third mounting hole 331 for allowing the stirring shaft 21 to pass through. One or more of the hollow body 31, the upper cover 32, and the lower cover 33 are provided with the through hole 34.

[0040] Figure 2 FIG. 3 shows a top view of the hollow body 31 provided in an embodiment of the present disclosure. Figure 2 As shown, the hollow body 31 further includes an inner wall 315, an outer wall 316, and a connecting plate 317. The inner wall 315 defines the first mounting hole 311 and is coaxially fixed to the stirring shaft 21. The outer wall 316 surrounds the outer circumference of the inner wall 315 and is spaced apart from the inner wall 315, thereby forming the second accommodating space 312 between the inner wall 315 and the outer wall 316. One or more connecting plates 317 are connected between the inner wall 315 and the outer wall 316 to secure the outer wall 316 to the inner wall 315.

[0041] like Figure 2 As shown, a net 35 (shown as a shadow) can be set inside the immobilized enzyme container 3, that is, inside the second accommodating space 312, and especially a net 35 is set at the boundary of the second accommodating space 312. The net 35 is used to further prevent the immobilized enzyme from leaving the second accommodating space 312. For example, the mesh size of the net 35 is smaller than the size of the through hole 34, so that even if the immobilized enzyme is damaged and becomes smaller, it cannot leave the second accommodating space 312.

[0042] In order to facilitate replacement of the immobilized enzyme in the immobilized enzyme container 3, the immobilized enzyme container 3 can be designed to be selectively opened. For example, the upper cover 32 and the upper opening 313 as well as the lower cover 33 and the lower opening 314 are both detachably connected.

[0043] Specifically, when the immobilized enzyme needs to be replaced, first separate the lower cover 33 from the lower opening 314 (see Figure 3 ), so that the old immobilized enzyme can leave the second accommodating space 312 from the lower opening 314, and then the lower cover 33 is reset upward, and then the upper cover 32 is separated from the upper opening 313 (see Figure 4), so that new immobilized enzyme can enter the second accommodating space 312 from the upper opening 313, and then the upper cover 32 is restored.

[0044] To ensure that the upper cover 32 of the immobilized enzyme container 3 does not separate from the upper opening 313 during rotation, a first locking assembly (not shown) is provided between the upper cover 32 and the hollow body 31. Similarly, a second locking assembly (not shown) is provided between the lower cover 33 and the hollow body 31.

[0045] It should be noted that the lock assembly can be any known lock in the prior art that can detachably connect two components.

[0046] In order to facilitate the separation of the upper cover 32 from the upper opening 313, the upper cover 32 has a first lifting ear (not shown). When the first locking assembly unlocks the upper cover 32 from the hollow body 31, the upper cover 32 can be separated upward from the upper opening 313 with the help of the first lifting ear. For example, the first lifting ear can be connected using a first rope 4, so that the upper cover 32 can be moved up / down by the first rope 4.

[0047] Similarly, in order to facilitate the separation of the lower cover 33 from the lower opening 314, the lower cover 33 has a second lifting ear (not shown). When the second locking assembly unlocks the lower cover 33 from the hollow body 31, the lower cover 33 can be separated downward from the lower opening 314 with the help of the second lifting ear. For example, the second lifting ear can be connected using a second rope 5 to move the lower cover 33 downward / upward through the second telescopic movement.

[0048] like Figure 1 As shown, an upper limit plate 6 and a lower limit plate 7 are provided on the stirring shaft 21. The upper limit plate 6 is located above the upper cover 32 and is used to limit the upper cover 32. The lower limit plate 7 is located below the lower cover 33 and is used to limit the lower cover 33.

[0049] Although the present disclosure has been described in detail above using general explanations, specific embodiments, and experiments, it will be apparent to those skilled in the art that modifications or improvements may be made based on the present disclosure. Therefore, such modifications or improvements, which do not depart from the spirit of the present disclosure, are intended to be within the scope of protection claimed herein.

Claims

1. An immobilized enzyme reaction device, characterized in that: The immobilized enzyme reaction device comprises: A reaction tank (1), wherein the reaction tank (1) comprises a first accommodating space (11); A stirring assembly (2), the stirring assembly (2) comprising a stirring shaft (21) and a stirring head (22) connected to the stirring shaft (21), the stirring shaft (21) and the stirring head (22) both being located in the first accommodating space (11); and An immobilized enzyme container (3), the immobilized enzyme container (3) being located in the first accommodating space (11), and the immobilized enzyme container (3) comprising: a first mounting hole (311), wherein the immobilized enzyme container (3) is coaxially fixed with the stirring shaft (21) through the first mounting hole (311); a second accommodating space (312), the second accommodating space (312) being configured to accommodate an immobilized enzyme; and A through hole (34), wherein the through hole (34) connects the second accommodating space (312) and the first accommodating space (11) to each other.

2. The immobilized enzyme reaction device according to claim 1, characterized in that: A net (35) is provided in the second accommodating space (312) for preventing the immobilized enzyme from leaving the second accommodating space (312).

3. The immobilized enzyme reaction device according to claim 1 or 2, characterized in that: The immobilized enzyme container (3) further comprises: A hollow body (31), the hollow body (31) comprising an upper opening (313), a lower opening (314) and the first mounting hole (311); an upper cover (32), the upper cover (32) covering the upper opening (313), and the upper cover (32) comprising a second mounting hole (321) for allowing the stirring shaft (21) to pass through; and a lower cover (33), the lower cover (33) covering the lower opening (314), and the lower cover (33) comprising a third mounting hole (331) for allowing the stirring shaft (21) to pass through; Wherein, one or more of the hollow body (31), the upper cover (32) and the lower cover (33) is provided with the through hole (34).

4. The immobilized enzyme reaction device according to claim 3, characterized in that: The hollow body (31) further comprises: an inner side wall (315), the inner side wall (315) defining the first mounting hole (311), the inner side wall (315) being coaxially fixed to the stirring shaft (21); an outer side wall (316), the outer side wall (316) surrounding the outer periphery of the inner side wall (315) and spaced apart from the inner side wall (315); and A connecting plate (317) is connected between the inner side wall (315) and the outer side wall (316).

5. The immobilized enzyme reaction device according to claim 3, characterized in that: The upper cover (32) and the upper opening (313) are detachably connected; The lower cover (33) and the lower opening (314) are detachably connected.

6. The immobilized enzyme reaction device according to claim 5, characterized in that: A first locking assembly is provided between the upper cover (32) and the hollow body (31).

7. The immobilized enzyme reaction device according to claim 6, characterized in that: The upper cover (32) has a first lifting ear, and when the first locking assembly unlocks the upper cover (32) from the hollow body (31), the upper cover (32) can be separated upward from the upper opening (313) by means of the first lifting ear.

8. The immobilized enzyme reaction device according to claim 5, characterized in that: A second locking assembly is provided between the lower cover (33) and the hollow body (31).

9. The immobilized enzyme reaction device according to claim 8, characterized in that: The lower cover (33) has a second lifting ear, and when the second locking assembly unlocks the lower cover (33) from the hollow body (31), the lower cover (33) can be separated downward from the lower opening (314) by means of the second lifting ear.

10. The immobilized enzyme reaction device according to claim 5, characterized in that: An upper limit plate (6) and a lower limit plate (7) are provided on the stirring shaft (21), wherein the upper limit plate (6) is located above the upper cover (32), and the lower limit plate (7) is located below the lower cover (33).