Agarose gel electrophoresis device

Abstract

The agarose gel electrophoresis device comprises a base, an electrophoresis tank is arranged in the base, an ultraviolet light source channel is formed in the bottom of the base, and the electrophoresis tank comprises an electrophoresis tank body, a separation fence card, a lane pool, an electrophoresis solution pool and a sample application tank. The interior of the electrophoresis tank body is divided into a plurality of groups of independent swimming lane pools through a plurality of groups of separation fence cards. The electrophoresis tank provided by the utility model can be separated into a plurality of mutually independent swimming lane pools, electrophoresis solution pools and sample application tanks through the separation fence cards, so that each sample can independently exist in the swimming lane pools and is separated in a physical separation manner, and mutual pollution in one swimming lane pool is avoided; after agarose is solidified, a recovery tank is formed through a comb, and after electrophoresis is carried out for a certain time, target nucleic acid is moved into the recovery tank and can be directly sucked and recovered, so that conventional gel cutting and gel recovery operations are reduced, the time and the consumption of reagent consumables are saved, the operation steps are reduced, and the recovery yield is increased.

Description

Technical Field

[0001] The utility model relates to the technical field of electrophoresis equipment, in particular to an agarose gel electrophoresis device. Background Art

[0002] Agarose gel electrophoresis is a standard method for separating, identifying, and purifying DNA fragments. Agarose, a hydrophilic but uncharged polysaccharide extracted from agar, serves as an excellent electrophoresis support. DNA carries a negative charge under alkaline conditions (pH 8.0 buffer) and migrates toward the positive electrode through the gel medium in an electric field. Different DNA fragments exhibit different electrophoretic rates in an electric field due to their molecular weight and conformational differences. Ethidium bromide (EB) can intercalate between base pairs in DNA molecules to form fluorescent complexes. After ultraviolet irradiation, distinct zones can be distinguished, achieving the purpose of separating and identifying molecular weights and screening for recombinants.

[0003] Existing electrophoresis equipment forms a whole piece of gel in an entire tank and then performs electrophoresis. This traditional electrophoresis equipment has no effect on the routine verification of extracted sample DNA, RNA or the success of PCR amplification. However, for samples that need to recover the target band, it is easy to contaminate other residual samples in the solution and adjacent samples. For this reason, we propose an agarose gel electrophoresis device. Utility Model Content

[0004] The purpose of the utility model is to provide an agarose gel electrophoresis device to solve the problems raised in the background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an agarose gel electrophoresis device, comprising a base, an electrophoresis tank is arranged inside the base, an ultraviolet light source channel is opened at the bottom of the base, the electrophoresis tank comprises an electrophoresis tank body, a partition fence card, a lane pool, an electrophoresis solution pool and a spotting tank, the electrophoresis tank body is arranged inside the base, the interior of the electrophoresis tank body is separated into multiple groups of independent lane pools by multiple groups of partition fence cards, the two ends of the electrophoresis tank body are separated by partition fence cards into electrophoresis solution pools and spotting tanks connected to the lane pools, four groups of combs for forming spotting tanks and recovery tanks are arranged inside the electrophoresis tank body, and two groups of electrode mechanisms are arranged on the base at positions corresponding to the electrophoresis solution pools.

[0006] Furthermore, two groups of the combs are inserted into the interior of the spotting tank to block the movement of agarose and form spotting positions, and the other two groups of the combs are inserted into the middle position of the electrophoresis tank body to form a recovery tank.

[0007] Furthermore, the electrode mechanism includes a connecting block, a fixing ring and a separator. The fixing rings are fixedly connected to both sides of the connecting block, and the separator is fixedly connected to the bottom of the connecting block at a position corresponding to the electrophoresis solution pool.

[0008] Furthermore, it also includes a cover plate, which is overlapped on the top of the base, and an avoidance groove is opened on the cover plate at a position corresponding to the separator.

[0009] Furthermore, a limiting groove that fits with the outer wall of the fixing ring is opened on the outer wall of the base at a position corresponding to the fixing ring, and a taking groove is opened on the top of the base.

[0010] Compared with the prior art, the present invention has the following beneficial effects: the electrophoresis tank provided in the present invention can be divided into multiple independent lane pools, electrophoresis solution pools and sample spotting pools by dividing fence cards. Such a setting can make each sample exist independently in the lane pool and be isolated by physical partitions to avoid mutual contamination in a lane pool. After the agarose solidifies, a recovery tank is formed by a comb. After a certain period of electrophoresis, the target nucleic acid moves to the inside of the recovery tank and can be directly absorbed and recovered, reducing the conventional gel cutting and gel recovery operations, saving time and reagent and consumables consumption, reducing the operation steps, and increasing the recovery rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 This is a schematic diagram of the first stereoscopic structure of the utility model;

[0012] Figure 2 This is a schematic diagram of the three-dimensional structure of the utility model after adding a cover plate;

[0013] Figure 3 This is a schematic diagram of the second stereoscopic structure of the utility model;

[0014] Figure 4 This is a schematic diagram of the three-dimensional structure of the electrophoresis tank of the utility model;

[0015] Figure 5 This is a schematic diagram of the three-dimensional structure of the electrode mechanism of the utility model;

[0016] Figure 6 This is a schematic diagram of the connection structure between the base and the electrophoresis tank in the utility model;

[0017] Figure 7 This is a schematic diagram of the three-dimensional structure of the cover plate of the utility model.

[0018] In the figure: 1 base, 2 electrophoresis tank, 3 cover, 4 electrode mechanism, 5 UV light source channel, 6 comb, 7 electrophoresis tank body, 8 partition fence card, 9 lane pool, 10 electrophoresis solution pool, 11 spotting slot, 12 connecting block, 13 fixing ring, 14 separator, 15 limiting slot, 16 taking slot, 17 avoidance slot. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0020] See also Figure 1-Figure 7 The utility model provides a technical solution: an agarose gel electrophoresis device, comprising a base 1, an electrophoresis tank 2 is arranged inside the base 1, an ultraviolet light source channel 5 is opened at the bottom of the base 1, the electrophoresis tank 2 comprises an electrophoresis tank body 7, a partition fence card 8, a lane pool 9, an electrophoresis solution pool 10 and a spotting tank 11, the electrophoresis tank body 7 is arranged inside the base 1, the interior of the electrophoresis tank body 7 is divided into multiple groups of independent lane pools 9 by multiple groups of partition fence cards 8, the two ends of the electrophoresis tank body 7 are separated by partition fence cards 8 into electrophoresis solution pools 10 and spotting tanks 11 connected to the lane pools 9, four groups of combs 6 for forming spotting tanks and recovery tanks are arranged inside the electrophoresis tank body 7, and two groups of electrode mechanisms 4 are arranged on the base 1 at the position corresponding to the electrophoresis solution pool 10.

[0021] Among them, the electrophoresis tank 2 can be divided into multiple independent lane pools 9, electrophoresis solution pools 10 and sample spotting tanks 11 by a partition fence card 8. Such a setting can make each sample exist independently in the lane pool 9, and be isolated by physical partitions to avoid mutual contamination in one lane pool 9. The electrophoresis solution pool 10 is used to add electrophoresis fluid and to place the motor mechanism 4.

[0022] See also Figure 1 、 Figure 2 and Figure 4 Two groups of the combs 6 are inserted into the interior of the spotting tank 11 to block the movement of agarose and form spotting positions, and the other two groups of the combs 6 are inserted into the middle position of the electrophoresis tank body 7 to form a recovery tank.

[0023] Among them, the comb 6 is placed when the agarose is injected. After the agarose solidifies, a recovery groove is formed by the two groups of combs 6 in the middle position. After a certain period of electrophoresis, the target nucleic acid moves to the inside of the recovery groove and can be directly absorbed and recovered, reducing the conventional gel cutting and gel recovery operations, saving time and reagent and consumables consumption, reducing the operation steps, and increasing the recovery rate. The spotting position formed by the comb 6 at the spotting groove 11 can be used to add nucleic acid samples.

[0024] See also Figure 1 、 Figure 4 、 Figure 5 and Figure 6 The electrode mechanism 4 includes a connecting block 12, a fixing ring 13 and a separator 14. The fixing ring 13 is fixedly connected to both sides of the connecting block 12. The separator 14 is fixedly connected to the bottom of the connecting block 12 at a position corresponding to the electrophoresis solution pool 10. The outer wall of the base 1 is provided with a limiting groove 15 that fits with the outer wall of the fixing ring 13 at a position corresponding to the fixing ring 13.

[0025] Among them, the set fixing ring 13 can be used to pass the positive and negative electrodes, and the set multiple groups of separators 4 can be inserted into the corresponding swimming lane pool 9 to place the positive and negative electrodes, and the limiting groove 15 set on the outer wall of the base 1 can fix the fixing ring 13 to prevent the electrode mechanism 4 from offset when working.

[0026] See also Figure 2 、 Figure 5 、 Figure 6 and Figure 7 , and also includes a cover plate 3, which is overlapped on the top of the base 1. An avoidance groove 17 is opened on the cover plate 3 and corresponds to the position of the separator 14. A taking groove 16 is opened on the top of the base 1.

[0027] The cover plate 3 can prevent electric leakage and liquid evaporation during electrophoresis, and the avoidance groove 17 is convenient for inserting the separator 14 into the swimming pool 9, and the avoidance groove 17 is convenient for removing the cover plate 3.

[0028] When in use, first, the electrophoresis tank 2 can be divided into multiple independent lane pools 9, electrophoresis solution pools 10 and spotting pools 11 by the partition fence card 8. Such a setting can make each sample exist independently in the lane pool 9, and be isolated by physical partitions to avoid mutual contamination in one lane pool 9. The electrophoresis solution pool 10 is used to add electrophoresis liquid and to place the motor mechanism 4. The comb 6 is placed when injecting agarose. After the agarose solidifies, a recovery tank is formed by the two groups of combs 6 in the middle position. After a certain period of electrophoresis, the target nucleic acid moves to the inside of the recovery tank and can be directly absorbed and recovered, which reduces the conventional gel cutting and gel recovery operations and saves time. The invention can save time and reagent consumables, reduce the operation steps, and increase the recovery rate. The spotting position formed by the comb 6 at the spotting slot 11 can be used to add nucleic acid samples. The fixed ring 13 can be used to pass positive and negative electrodes, and the multiple groups of separators 4 can be inserted into the corresponding swimming lane pool 9 for placing positive and negative electrodes. The limiting groove 15 set on the outer wall of the base 1 can fix the fixed ring 13 to prevent the electrode mechanism 4 from shifting when working. The cover plate 3 can prevent leakage and liquid evaporation during the electrophoresis operation, and the avoidance groove 17 is convenient for the separator 14 to be inserted into the swimming lane pool 9, and the avoidance groove 17 is also convenient for removing the cover plate 3.

[0029] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An agarose gel electrophoresis apparatus, comprising a base (1), characterized in that: An electrophoresis tank (2) is provided inside the base (1), and an ultraviolet light source channel (5) is provided at the bottom of the base (1). The electrophoresis tank (2) comprises an electrophoresis tank body (7), a partition fence card (8), a swimming lane pool (9), an electrophoresis solution pool (10) and a sample spotting tank (11). The electrophoresis tank body (7) is provided inside the base (1), and the inside of the electrophoresis tank body (7) is divided into a plurality of independent swimming lane pools (9) by a plurality of partition fence cards (8). The electrophoresis tank body (7) is divided into an electrophoresis solution pool (10) and a sample spotting tank (11) at both ends by the partition fence cards (8) and the like, which are connected to the swimming lane pool (9). Four groups of combs (6) for forming a sample spotting tank and a recovery tank are provided inside the electrophoresis tank body (7), and two groups of electrode mechanisms (4) are provided on the base (1) at positions corresponding to the electrophoresis solution pool (10).

2. An agarose gel electrophoresis apparatus according to claim 1, characterized in that: Two groups of the combs (6) are inserted into the interior of the spotting tank (11) to block the movement of agarose and form spotting positions, and the other two groups of the combs (6) are inserted into the middle position of the electrophoresis tank body (7) to form a recovery tank.

3. An agarose gel electrophoresis apparatus according to claim 2, characterized in that: The electrode mechanism (4) comprises a connecting block (12), a fixing ring (13) and a separator (14); the fixing rings (13) are fixedly connected to both sides of the connecting block (12); and the separator (14) is fixedly connected to the bottom of the connecting block (12) at a position corresponding to the electrophoresis solution pool (10).

4. An agarose gel electrophoresis apparatus according to claim 3, characterized in that: It also includes a cover plate (3), the cover plate (3) being connected to the top of the base (1), and an avoidance groove (17) being provided on the cover plate (3) at a position corresponding to the separation plate (14).

5. An agarose gel electrophoresis apparatus according to claim 4, characterized in that: A limiting groove (15) is provided on the outer wall of the base (1) at a position corresponding to the fixing ring (13), and is fitted with the outer wall of the fixing ring (13). A taking groove (16) is provided on the top of the base (1).

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