EB virus related tumor marker detection kit based on EBNA1

Abstract

An EB virus related tumor marker detection kit based on EBNA1 relates to the technical field of virus detection, and comprises a box body, a cover, an elisa plate, a shell and a shelf, the cover is arranged at an opening in the upper part of the box body, the elisa plate is arranged in the box body, the shell is arranged below the elisa plate, one end of the shell penetrates through the side surface of the box body, the shelf is arranged in the shell, and the elisa plate is arranged in the shelf. An EB virus detection reagent is placed on the storage rack; the ELISA plate is high in practicability and very convenient to use, and the sealing performance of a sealing film and a test tube on the ELISA plate is improved while the sealing film is conveniently positioned by arranging the pressing plate on the ELISA plate; through the rotatable storage rack, each reagent bottle above the rotatable storage rack can be fully shaken conveniently, so that solutions in the reagent bottles are fully and uniformly mixed.

Description

Technical Field

[0001] The utility model relates to the technical field of virus detection, in particular to an EBNA1-based EB virus-related tumor marker detection kit. Background Art

[0002] Epstein-Barr virus (EBV) is the causative agent of infectious mononucleosis. EBV is also closely associated with the development of nasopharyngeal carcinoma and childhood lymphoma, and is listed as a potentially carcinogenic human tumor virus. EBNA1 is an antigen that appears early in EBV-infected cells and plays a crucial role in regulating viral gene replication and transcription. It persists in the final stages of the viral replication cycle. Therefore, EBNA1 testing can be an important tool for diagnosing EBV infection and related disease states.

[0003] In clinical practice, the commonly used EBNA1 detection reagent is mainly ELISA detection reagent. Compared with traditional methods, it is simpler, faster, and the test results are easy to judge. However, the reagent bottle needs to be shaken before testing to fully mix it for easy use in subsequent testing. In addition, the operation steps of the test kit are relatively cumbersome when using it. If the seal is not strict during the transfer process, it is easy for the test liquid to contaminate each other, affecting the accuracy of subsequent testing. Utility Model Content

[0004] In order to overcome the shortcomings of the background technology, the present invention discloses an EB virus-related tumor marker detection kit based on EBNA1. The present invention provides a lid, an ELISA plate, a shell and a storage rack on the box body to facilitate the clinical detection of EB virus-related tumor markers.

[0005] In order to achieve the purpose of the utility model, the utility model adopts the following technical solutions:

[0006] An EBNA1-based Epstein-Barr virus-related tumor marker detection kit includes a box body, a lid, an ELISA plate, a shell and a storage rack. The lid is provided at the upper opening of the box body, the ELISA plate is provided in the box body, and the shell is provided below the ELISA plate. One end of the shell passes through the side of the box body, and a storage rack is provided in the shell. The EB virus detection reagent is placed on the storage rack.

[0007] The ELISA plate is provided with fixed holes in an equidistant array, the four corners of the upper surface of the ELISA plate are provided with clamping blocks, one end of the upper surface of the ELISA plate is provided with a pressing plate, and one side of the lower surface of the ELISA plate is provided with two limiting rods.

[0008] The pressing plate is a U-shaped structure, one end of which is hinged to the upper surface of the enzyme labeling plate, and the four corners of the lower surface of the pressing plate are distributed with clamping holes, which are matched with the clamping blocks, and a sealing film is provided between the pressing plate and the enzyme labeling plate.

[0009] Positioning holes are distributed at the four corners of the sealing film.

[0010] The upper end of the limiting rod is provided with a slider which is a T-shaped block or a dovetail block. The slider is slidably connected with the T-shaped slot or dovetail slot on the lower surface of the ELISA plate.

[0011] Two support rods are symmetrically provided at one end of the lower surface of the shell, and a storage rack is provided in the shell. The storage rack includes a bearing plate, a first fixing ring, a connecting plate, a connecting rod, a second fixing ring and a spring. The bearing plate is arranged on the bottom surface of the inner wall of the shell, and a mounting hole is provided in the center of the upper surface of the bearing plate. A first fixing ring is provided at the upper end of the inner wall of the mounting hole. Card slots are distributed in an equidistant array on the upper surface of the bearing plate, and each card slot is arranged in a ring with the mounting hole as the center of the circle. The connecting plate is arranged above the bearing plate, and a connecting rod is provided in the center of the lower surface of the connecting plate. The lower end of the connecting rod passes through the first fixing ring and is arranged in the mounting hole. A second fixing ring is provided at the lower end of the connecting rod. A spring is provided between the second fixing ring and the first fixing ring, and the spring is sleeved on the connecting rod.

[0012] A swivel is provided on the side of the connecting plate, which is sleeved on the connecting plate. The side of the swivel passes through the side of the shell. Connecting holes are distributed on the upper surface of the connecting plate, and each detection reagent bottle is installed in the connecting hole. Rollers are distributed on the lower surface of the connecting plate. Two limit holes are provided on the upper surface of the connecting plate, and the lower end of the limit rod is set in the corresponding limit hole.

[0013] The utility model discloses an EB virus-related tumor marker detection kit based on EBNA1. The utility model has strong practicality and is very convenient to use. By arranging a pressure plate on the ELISA plate, the positioning of the sealing film is facilitated while increasing the sealing performance between the sealing film and the test tubes on the ELISA plate. The rotatable storage rack facilitates the full shaking of the reagent bottles above it, so that the solutions in the reagent bottles are fully mixed. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0015] Figure 2 This is a schematic diagram of the three-dimensional structure of the enzyme labeling plate of the present utility model;

[0016] Figure 3 This is a three-dimensional structural diagram of the housing and the storage rack of the utility model;

[0017] Figure 4 This is a cross-sectional view of the housing and storage rack of the present invention;

[0018] Figure 5 This is a top view of the sealing membrane of the present invention;

[0019] In the figure: 1. Box body; 2. Lid; 3. ELISA plate; 4. Shell; 5. Pressing plate; 6. Fixing hole; 7. Block; 8. Clamping hole; 9. Slider; 10. Limiting rod; 11. Support rod; 12. Connecting plate; 13. Rotating ring; 14. Connecting hole; 15. Roller; 16. Limiting hole; 17. Mounting hole; 18. Loading plate; 19. Clamping slot; 20. Connecting rod; 21. First fixing ring; 22. Second fixing ring; 23. Spring; 24. Sealing membrane; 25. Positioning hole. DETAILED DESCRIPTION

[0020] The present invention will be explained in detail through the following embodiments. The purpose of disclosing the present invention is to protect all technical improvements within the scope of the present invention.

[0021] Combined with attachment Figures 1 to 5 An EBNA1-based Epstein-Barr virus-related tumor marker detection kit includes a box body 1, a lid 2, an ELISA plate 3, a shell 4 and a storage rack. The lid 2 is provided at the upper opening of the box body 1, the ELISA plate 3 is provided inside the box body 1, and the shell 4 is provided below the ELISA plate 3. One end of the shell 4 passes through the side of the box body 1. The shell 4 is provided inside the shell 4, and the EB virus detection reagent is placed on the storage rack.

[0022] The ELISA plate 3 has fixing holes 6 arranged in an equidistant array, and blocks 7 are distributed at the four corners of the upper surface of the ELISA plate 3 . A pressing plate 5 is provided at one end of the upper surface of the ELISA plate 3 , and two limiting rods 10 are provided on one side of the lower surface of the ELISA plate 3 .

[0023] The pressing plate 5 is a U-shaped structure. One end of the pressing plate 5 is hinged to the upper surface of the ELISA plate 3. The four corners of the lower surface of the pressing plate 5 are provided with clamping holes 8. The clamping holes 8 are arranged in conjunction with the clamping blocks 7. A sealing film 24 is provided between the pressing plate 5 and the ELISA plate 3. The ELISA plate and the pressing plate 5 cooperate to fit the sealing film 24 to the mouth of each test tube to prevent the liquid in the test tube from overflowing and contaminating each other.

[0024] Positioning holes 25 are distributed at the four corners of the sealing film 24 , and the positioning holes 25 are arranged in cooperation with the clamping blocks 7 to facilitate the covering of the sealing film 24 .

[0025] The upper end of the limiting rod 10 is provided with a slider 9, which is a T-block or a dovetail block. The slider 9 is slidably connected to the T-slot or dovetail slot on the lower surface of the ELISA plate 3. After taking out the ELISA plate 3, the limiting rod 10 is removed.

[0026] Two support rods 11 are symmetrically provided at one end of the lower surface of the shell 4, and the support rod 11 is hinged to the shell. A storage rack is provided in the shell 4, and the storage rack includes a carrying plate 18, a first fixing ring 21, a connecting plate 12, a connecting rod 20, a second fixing ring 22 and a spring 23. The carrying plate 18 is arranged on the bottom surface of the inner wall of the shell 4, and a mounting hole 17 is provided at the center of the upper surface of the carrying plate 18. A first fixing ring 21 is provided at the upper end of the inner wall of the mounting hole 17, and a card slot 19 is distributed in an equidistant array on the upper surface of the carrying plate 18. Each card slot 19 is arranged in a ring with the mounting hole 17 as the center of the circle. The connecting plate 12 is arranged above the carrying plate 18, and a connecting rod 20 is provided at the center of the lower surface of the connecting plate 12. The lower end of the connecting rod 20 passes through the first fixing ring 21 and is arranged in the mounting hole 17. A second fixing ring 22 is provided at the lower end of the connecting rod 20, and a spring 23 is provided between the second fixing ring 22 and the first fixing ring 21, and the spring 23 is sleeved on the connecting rod 20.

[0027] The side of the connecting plate 12 is provided with a rotating ring 13, which is sleeved on the connecting plate 12, and the side of the rotating ring 13 passes through the side of the shell 4. The upper surface of the connecting plate 12 is provided with connecting holes 14, and each detection reagent bottle is installed in the connecting hole 14. The lower surface of the connecting plate 12 is provided with a roller 15. Rotating the rotating ring 13 causes the connecting plate 12 to rotate above the supporting plate 18, and the roller 15 rotates from one slot 19 to another slot 19, thereby driving the connecting plate 12 to move up and down above the supporting plate 18, so that the liquid in each detection reagent bottle can be shaken for sufficient mixing. The upper surface of the connecting plate 12 is provided with two limiting holes 16, and the lower end of the limiting rod 10 is set in the corresponding limiting hole 16 to prevent the connecting plate 12 from rotating in the shell 4 before the test kit is unopened.

[0028] The EBNA1-based EB virus-related tumor marker detection kit described in the embodiment is first unsealed when in use, the ELISA plate 3 is taken out, the limit rod 10 is removed, the shell 4 is pulled out from one side of the box body 1, and the two support rods 11 are flipped downward to support the shell 4, each detection reagent bottle is taken out and allowed to equilibrate at room temperature for 30 minutes, and then each detection reagent bottle is installed in the corresponding connection hole 14, the rotating ring 13 is rotated to make the connecting plate 12 rotate above the carrying plate 18, and the roller 15 is rotated from a slot 19 Rotate into another card slot 19, thereby driving the connecting plate 12 to move up and down above the carrying plate 18, so that the liquid in each detection reagent bottle can be shaken for full mixing, and then the test can be carried out. After the reagent is added, flip the pressing plate 5 upward, place the sealing film 24 on the ELISA plate 3, so that the card block 7 passes through the corresponding positioning hole 25, and then cover the pressing plate 5 on the sealing film 24. The ELISA plate and the pressing plate 5 cooperate to fit the sealing film 24 to the mouth of each test tube to prevent the liquid in the test tube from overflowing and contaminating each other, and then the subsequent steps can be carried out.

[0029] The parts of the present invention that are not described in detail are prior art. Although the present invention is specifically demonstrated and introduced in conjunction with the preferred implementation scheme, there are many methods and ways to implement the technical solution. The above is only a preferred implementation scheme of the present invention. However, technical personnel in the relevant field should understand that various changes can be made to the form and details of the present invention without departing from the spirit and scope of the present invention as defined by the attached claims, and all of them are within the scope of protection of the present invention.

Claims

1. A kit for detecting EB virus-related tumor markers based on EBNA1, comprising a box body (1), a cover (2), an ELISA plate (3), a shell (4) and a shelf, wherein the cover (2) is provided at the upper opening of the box body (1), and the kit is characterized in that: An enzyme labeling plate (3) is provided in the box body (1), a shell (4) is provided below the enzyme labeling plate (3), one end of the shell (4) passes through the side of the box body (1), a storage rack is provided in the shell (4), and an EB virus detection reagent is placed on the storage rack.

2. The EB virus-related tumor marker detection kit based on EBNA1 according to claim 1, characterized in that: The ELISA plate (3) is provided with fixed holes (6) in an equidistant array, and blocks (7) are provided at the four corners of the upper surface of the ELISA plate (3). A pressing plate (5) is provided at one end of the upper surface of the ELISA plate (3), and two limiting rods (10) are provided on one side of the lower surface of the ELISA plate (3).

3. The EB virus-related tumor marker detection kit based on EBNA1 according to claim 2, characterized in that: The pressing plate (5) is a U-shaped structure. One end of the pressing plate (5) is hinged to the upper surface of the ELISA plate (3). Clamping holes (8) are distributed at the four corners of the lower surface of the pressing plate (5). The clamping holes (8) are arranged in conjunction with the clamping blocks (7). A sealing film (24) is provided between the pressing plate (5) and the ELISA plate (3).

4. The EBNA1-based EB virus-related tumor marker detection kit according to claim 3, characterized in that: Positioning holes (25) are distributed at the four corners of the sealing film (24).

5. The EB virus-related tumor marker detection kit based on EBNA1 according to claim 2, characterized in that: The upper end of the limiting rod (10) is provided with a slider (9), which is a T-shaped block or a dovetail block. The slider (9) is slidably connected to the T-shaped groove or dovetail groove on the lower surface of the ELISA plate (3).

6. The EB virus-related tumor marker detection kit based on EBNA1 according to claim 1, characterized in that: Two support rods (11) are symmetrically provided at one end of the lower surface of the shell (4), and the storage rack includes a bearing plate (18), a first fixing ring (21), a connecting plate (12), a connecting rod (20), a second fixing ring (22) and a spring (23). The bearing plate (18) is arranged on the bottom surface of the inner wall of the shell (4), and a mounting hole (17) is provided at the center of the upper surface of the bearing plate (18). The upper end of the inner wall of the mounting hole (17) is provided with a first fixing ring (21), and the upper surface of the bearing plate (18) is equidistantly arrayed with card slots ( 19), each slot (19) is arranged in an annular shape with the mounting hole (17) as the center, the connecting plate (12) is arranged above the bearing plate (18), a connecting rod (20) is provided at the center of the lower surface of the connecting plate (12), the lower end of the connecting rod (20) passes through the first fixing ring (21) and is arranged in the mounting hole (17), a second fixing ring (22) is provided at the lower end of the connecting rod (20), a spring (23) is provided between the second fixing ring (22) and the first fixing ring (21), and the spring (23) is sleeved on the connecting rod (20).

7. The EB virus-related tumor marker detection kit based on EBNA1 according to claim 6, characterized in that: The side of the connecting plate (12) is provided with a rotating ring (13), which is sleeved on the connecting plate (12). The side of the rotating ring (13) passes through the side of the shell (4). The upper surface of the connecting plate (12) is provided with connecting holes (14), and each detection reagent bottle is installed in the connecting hole (14). The lower surface of the connecting plate (12) is provided with a roller (15). The upper surface of the connecting plate (12) is provided with two limiting holes (16), and the lower end of the limiting rod (10) is set in the corresponding limiting hole (16).

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