Automatic sampling mechanism of flow cytometer

Abstract

The utility model relates to the technical field of cytometers, and discloses an automatic sampling mechanism of a flow cytometer, which comprises a bottom plate, a translation plate capable of horizontally moving is connected onto the bottom plate through a translation component, and a bearing component capable of rotating around the axis of the bearing component is connected onto the translation plate through a rotating component. A material loading part for clamping a test tube is mounted on the bearing part, and a uniform mixing assembly for uniformly mixing a sample in the test tube is also arranged on the bottom plate; the rotating assembly is mounted on the translation plate and can ensure that the bearing part can translate along a specified direction and can rotate around the axis of the bearing part, so that the material loading part can be automatically conveyed to the lower part of a corresponding sampling needle after clamping a test tube, and the interior of the test tube is mixed through the uniform mixing assembly; the operation precision and efficiency of the mechanism are both higher than those of manual operation, the labor intensity can be reduced, the tedious manual operation is liberated, the sample detection efficiency of flow cytometry is improved, and rapid sample loading and detection of the flow cytometry on samples are realized.

Description

Technical Field

[0001] The utility model relates to the technical field of cytometers, in particular to an automatic sample introduction mechanism for a flow cytometer. Background Art

[0002] Currently, the sampling structures of flow cytometers can be divided into manual sampling and automatic sampling. The former is to manually place the flow tube into the carrier directly below the sampling needle of the flow cytometer, and the sampling needle descends for sampling; the latter is to place the flow tube into the automatic sampler, and the sample is automatically transferred to the bottom of the sampling needle for sampling; these two sampling structures determine the complexity and size of the sampling structure.

[0003] Early flow cytometer products mainly used manual injection. When a large amount of samples were collected or 96-well plate injection was required, the shortcomings of manual injection, such as many steps in the operation process, poor accuracy, prone to errors, low efficiency, and inability to use 96-well plates, became apparent. Therefore, automatic injection is the main method used in the market.

[0004] Most manufacturers use an XY-axis injection method, which results in a larger structure. Some manufacturers use a combination of X-axis motion and platform rotation, resulting in a smaller size but incompatibility with 96-well plates. XY-axis injection methods often employ aspiration mixing or needle vibration, requiring more complex host performance and resulting in less effective mixing than vortex mixing. Due to the limitations of injector performance, some manufacturers require higher dimensional accuracy for flow tubes or 96-well plates, resulting in poor compatibility.

[0005] Based on this, in order to improve the sample detection efficiency of flow cytometry, free up tedious manual operations, and realize rapid sample loading and detection of flow cytometer, the utility model proposes an automatic sample introduction mechanism for flow cytometer. Utility Model Content

[0006] The purpose of the utility model is to overcome the deficiencies in the prior art and provide an automatic sample introduction mechanism for a flow cytometer. The mechanical operation accuracy and efficiency are higher than those of manual operation, which can reduce labor intensity, free up manual labor from tedious operations, speed up testing, improve the sample detection efficiency of flow cells, and realize rapid sample loading and detection of flow cytometers.

[0007] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0008] A flow cytometer automatic sample introduction mechanism includes a base plate, a translation plate capable of horizontal movement is connected to the base plate via a translation assembly, a turntable capable of rotating about its own axis is connected to the translation plate via a rotation assembly, a loading component for clamping a test tube is installed on the turntable, and a mixing component for mixing the sample in the test tube is also provided on the base plate.

[0009] Optionally, the translation assembly includes two slide rails arranged opposite to each other, and sliders are slidably installed on both slide rails. The two sliders are fixedly connected by a connecting plate, and the translation plate is fixedly installed on the connecting plate; a linear module parallel to the slide rails is arranged between the two sliders, and the output end of the linear module is connected to the connecting plate.

[0010] Optionally, the rotating assembly includes a support plate and a rotating shaft, the rotating shaft is rotatably installed on the support plate, and the support plate is fixedly connected to the translation plate through a pillar; a first motor is also installed on the translation plate, the output end of the first motor is connected to a driving wheel, and a driven wheel is installed at the lower end of the rotating shaft, and a synchronous belt is provided on the outer side of the driving wheel and the driven wheel, and the upper end of the rotating shaft is fixedly connected to the turntable.

[0011] Optionally, the mixing assembly includes a first guide rail perpendicular to the bottom plate, and a mixer that can be raised and lowered is slidably mounted on the first guide rail. The top end of the mixer can rotate and is provided with a groove corresponding to the bottom contour of the test tube.

[0012] Optionally, the mixing assembly also includes a second motor mounted on the base plate, the output end of the second motor is connected to a screw rod parallel to the first guide rail, a nut is screwed onto the screw rod, a connecting frame is mounted on the nut, and the end of the connecting frame is connected to the mixer.

[0013] Optionally, the loading component is a test tube tray, and the lower part of the test tube tray is sleeved on the turntable.

[0014] Optionally, a plurality of test tube grooves are provided on the outer circumferential surface of the test tube plate, a bottom hole is provided at the bottom of the test tube groove, and the bottom of the test tube can pass through the bottom hole and contact the top groove of the mixer.

[0015] Optionally, the outer side of the turntable is provided with a concave positioning groove, the lower part of the test tube tray is provided with positioning parts corresponding to the position and number of the positioning groove, and the positioning parts are embedded in the positioning groove.

[0016] Optionally, an adapter is installed on the turntable, and the loading component adopts a culture plate; a first mounting groove for mounting the culture plate is provided on the top of the adapter, and an adjustment hole for mounting an adjustment screw is provided on the side wall of the first mounting groove.

[0017] Optionally, a shell is provided above the base plate, the translation assembly and the rotation assembly are both located in the shell, and the turntable and the loading component are located above the shell; a support block is provided on one side of the shell, a cover body that can be flipped longitudinally is installed on the top of the support block, and the cover body and the support block are connected by a hinge.

[0018] Beneficial effects

[0019] (1) In the present invention, the translation plate can be driven by the translation assembly to move horizontally, and the receiving part can be driven by the rotation assembly to rotate. The rotation assembly is installed on the translation plate, which can ensure that the receiving part can not only translate in the specified direction but also rotate around its own axis, so that the loading part can automatically transport the test tube to the bottom of the corresponding sampling needle after clamping it, and mix the test tube through the mixing assembly; the operation accuracy and efficiency of this mechanism are higher than those of manual operation, which can reduce labor intensity, free up manual tedious operations, improve the sample detection efficiency of flow cytometry, and realize rapid sample loading and detection of flow cytometer.

[0020] (2) In the present invention, after the adapter is installed on the turntable, it can be compatible with most culture plates by adjusting the fit between the screw and the adjustment hole.

[0021] (3) In the present invention, after the mixer rises to contact the bottom of the corresponding test tube, the rotation of its top end drives the test tube to rotate in the test tube slot, mixing the sample in the test tube in a vortex mixing manner, thereby achieving a better mixing effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic structural diagram of the automatic sampling mechanism of the flow cytometer according to the embodiment of the present invention;

[0023] Figure 2 This is a schematic diagram of the position structure of the translation component and the rotation component in the embodiment of the utility model;

[0024] Figure 3 This is a schematic structural diagram of a translation assembly in an embodiment of the present utility model;

[0025] Figure 4 This is a schematic structural diagram of a rotating assembly in an embodiment of the present utility model;

[0026] Figure 5 This is a schematic structural diagram of a mixing assembly in an embodiment of the present utility model;

[0027] Figure 6 This is a schematic structural diagram of a test tube tray in an embodiment of the present utility model;

[0028] Figure 7 This is a schematic diagram of the assembly structure of the test tube tray and the turntable in the embodiment of the utility model;

[0029] Figure 8 This is a schematic structural diagram of the housing in an embodiment of the present utility model;

[0030] Figure 9This is a schematic diagram of the exploded structure of the adapter and the culture plate in the embodiment of the utility model;

[0031] Figure 10 This is a schematic structural diagram of an adapter in an embodiment of the present utility model;

[0032] Figure 11 This is a schematic structural diagram of the sampling mechanism in an embodiment of the present utility model;

[0033] Among them, 1. bottom plate; 2. translation plate;

[0034] 3. Translation assembly; 31. Slide rail; 32. Slider; 33. Connecting plate; 34. Linear module;

[0035] 4. Rotating assembly; 41. Support plate; 42. Pillar; 43. Rotating shaft; 44. First motor; 45. Driven pulley; 46. Driving pulley; 47. Synchronous belt;

[0036] 5. Mixing assembly; 51. Second motor; 52. Screw; 53. Nut; 54. Connecting frame; 55. First guide rail; 56. Mixer;

[0037] 6. Turntable; 61. Positioning slot;

[0038] 7. Test tube tray; 71. Test tube slot; 72. Bottom hole; 73. Handle; 74. Positioning pin; 75. Positioning portion;

[0039] 81. Shell; 82. Support block; 83. Cover;

[0040] 9. Sampling mechanism; 91. Fixing plate; 92. Second guide rail; 93. Assembly; 94. Sampling needle; 95. Cleaning block; 96. Drive assembly;

[0041] 10. Protective plate; 11. Avoidance groove; 12. Adapter; 13. First installation groove; 14. Adjustment hole; 15. Floating shielding plate; 16. Culture plate. DETAILED DESCRIPTION

[0042] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. These drawings are simplified schematic diagrams that only illustrate the basic structure of the present invention in a schematic manner, and therefore only show components related to the present invention.

[0043] Example 1

[0044] like Figure 1-Figure 2 、 Figure 6-Figure 8 、 Figure 11As shown, an automatic sampling mechanism of a flow cytometer includes a base plate 1, a translation plate 2 and a turntable 6 arranged in sequence from bottom to top. The three are parallel to each other, and a translation component 3 capable of driving the translation plate 2 to move horizontally is installed on the base plate 1, a rotating component 4 capable of driving the turntable 6 to rotate around its own axis is installed on the translation plate 2, and a test tube tray 7 is provided on the turntable 6 for clamping test tubes.

[0045] As mentioned above, a corresponding sampling mechanism 9 is usually provided on one side of the sample feeding mechanism. Under the joint action of the translation component 3 and the rotation component 4, the test tube tray 7 can not only translate in a specified direction, but also rotate around its own axis, so that the test tubes on the test tube tray 7 can enter the working range of the sampling mechanism 9 in turn for relevant sampling or sample addition work.

[0046] The operation accuracy and efficiency of the sample injection mechanism are higher than those of manual operation, which can reduce labor intensity, free up manual labor from tedious operations, improve the sample detection efficiency of flow cytometry, and realize rapid sample loading and detection of flow cytometers.

[0047] The sampling mechanism 9 includes a fixed plate 91, on one side of which is installed a second guide rail 92 perpendicular to the base plate 1, on which an assembly portion 93 is slidably installed, on which a sampling needle 94 is installed, and a cleaning block 95 is provided below the assembly portion 93, and the lower end of the sampling needle 94 can be movably passed through the cleaning block 95, and a driving assembly 96 for driving the assembly portion 93 to rise and fall is also installed on the fixed plate 91, which is driven by a belt drive so that the sampling needle 94 can be smoothly raised and lowered; the sampling mechanism 9 here is prior art and will not be described in detail here.

[0048] Furthermore, the test tube tray 7 is used as a loading component, and its lower part is sleeved on the turntable 6. When taking it, it is only necessary to lift the test tube tray 7 upwards to separate it from the turntable 6, so as to facilitate quick loading and replacement.

[0049] An inward-concave positioning groove 61 is provided on the outer side of the turntable 6, and a positioning portion 75 corresponding to the position and number of the positioning groove 61 is provided on the lower part of the test tube tray 7. The positioning portion 75 is embedded in the positioning groove 61 to achieve positioning and drive the test tube tray 7 and the turntable 6 to rotate synchronously.

[0050] A handle 73 is also fixedly installed on the top of the test tube tray 7, which can facilitate lifting the test tube tray 7. The test tube tray 7 is connected to the handle 73 by a screw that penetrates the test tube tray 7 from bottom to top, and a positioning pin 74 for positioning is also provided between the handle 73 and the test tube tray 7. The positioning pin 74 is parallel to the above-mentioned screw to prevent the handle 73 from rotating.

[0051] A plurality of test tube slots 71 are provided on the outer circumferential surface of the test tube tray 7, and a bottom hole 72 is provided at the bottom of the test tube slot 71. The arc length of the cross section of the test tube slot 71 is greater than half of the outer circumference of the test tube cross section, and the diameter of the bottom hole 72 is smaller than the outer diameter of the test tube. Therefore, the test tube can only be inserted into the test tube slot 71 from top to bottom, avoiding the risk of it being thrown from the side, and a part of the bottom of the test tube will pass through the bottom hole 72 to the bottom thereof.

[0052] like Figure 1-Figure 3 As shown, the translation assembly 3 includes two slide rails 31 arranged opposite to each other, and sliders 32 are slidably installed on the two slide rails 31. The two sliders 32 are fixedly connected by a connecting plate 33, and the translation plate 2 is fixedly installed on the connecting plate 33; a linear module 34 parallel to the slide rails 31 is arranged between the two sliders 32, and the output end of the linear module 34 is connected to the connecting plate 33.

[0053] The linear module 34 adopts the existing screw drive structure, and the motor at its input end is connected to the screw through a belt transmission structure, so that the output end of the linear module 34 can move linearly in a direction parallel to the slide rail 31, and then drive the translation plate 2 to achieve horizontal movement through the connecting plate 33; the sliders 32 on the two slide rails 31 are connected by the connecting plate 33, which can enhance the stability of the operation of the component.

[0054] like Figure 1-Figure 2 、 Figure 4 As shown, the rotating assembly 4 includes a support plate 41 and a rotating shaft 43. The rotating shaft 43 is rotatably arranged on the support plate 41, and the support plate 41 is fixedly connected to the translation plate 2 through a pillar 42; a first motor 44 is also installed on the translation plate 2, and the output end of the first motor 44 is connected to a driving wheel 46, and a driven wheel 45 is installed at the lower end of the rotating shaft 43. The outer sides of the driving wheel 46 and the driven wheel 45 are matched with a synchronous belt 47, and the upper end of the rotating shaft 43 is fixedly connected to the turntable 6.

[0055] The rotating shaft 43 is passed through the support plate 41, and the two are connected by a bearing. The torque output by the first motor 44 is transmitted to the rotating shaft 43 through the belt transmission structure composed of the driven wheel 45, the driving wheel 46 and the synchronous belt 47, which can drive the rotating shaft 43 to rotate around its own axis, and then drive the turntable 6 at its upper end to rotate.

[0056] Since the rotating assembly 4 moves together with the translation plate 2 , the bottom of the first motor 44 is in a suspended state. In order to avoid interference between it and the bottom plate 1 below, an avoidance groove 11 is opened on the bottom plate 1 at a position corresponding to the first motor 44 .

[0057] like Figure 2 、 Figure 5 As shown, a mixing component 5 for mixing the sample in the test tube is also provided on the bottom plate 1.

[0058] The mixing assembly 5 includes a first guide rail 55 perpendicular to the bottom plate 1, on which a mixer 56 capable of lifting and lowering is slidably mounted. The top of the mixer 56 is rotatable and has a groove corresponding to the bottom contour of the test tube.

[0059] The mixing assembly 5 also includes a second motor 51 installed on the base plate 1. The output end of the second motor 51 is connected to a screw rod 52 parallel to the first guide rail 55. A nut 53 is screwed onto the screw rod 52. A connecting frame 54 is installed on the nut 53, and the end of the connecting frame 54 is connected to the mixer 56.

[0060] As described above, the mixer 56 includes a driving motor and a rotating part. The rotating part is installed at the output end of the driving motor and is perpendicular to the bottom plate 1. The groove is opened at the top of the rotating part. Driven by the second motor 51 and the screw rod 52 and other components, the mixer 56 can be driven to rise and fall along the first guide rail 55 through the connecting frame 54. When the mixer 56 rises to the specified position, the groove at the top of the rotating part contacts the bottom of the corresponding test tube, and drives the test tube to rotate around its own axis in the test tube slot 71, mixing the sample in the test tube in a vortex mixing manner, thereby achieving a better mixing effect.

[0061] Example 2

[0062] like Figure 1-Figure 2 、 Figure 8 As shown, on the basis of embodiment 1, a shell 81 is provided above the base plate 1, the translation assembly 3 and the rotation assembly 4 are both located in the shell 81, and the turntable 6 and the loading component are located above the shell 81; a support block 82 is provided on one side of the shell 81, and a cover body 83 that can be flipped longitudinally is installed on the top of the support block 82, and the cover body 83 and the support block 82 are connected by a hinge.

[0063] A protective plate 10 is fixedly installed on the support plate 41. The protective plate 10 is located in the shell 81 and can move synchronously with the support plate 41. At the same time, a floating shielding plate 15 is also fixedly installed in the shell 81. A through hole is opened on the floating shielding plate 15 to facilitate the movement and extension of the corresponding components.

[0064] The shell 81 and the support block 82 are integrally formed and connected, and the cover body 83 adopts a translucent window. Under the action of the hinge, the cover body 83 can be flipped longitudinally and blocked on the outside of the test tube tray 7. After the cover body 83 is flipped downward, one side thereof is open to facilitate the horizontal movement of the test tube tray 7.

[0065] Example 3

[0066] like Figure 2 、 Figure 9-10As shown, based on the first and second embodiments, the sample injection mechanism can also be adapted to 96-well culture plates 16 of various specifications, that is, an adapter 12 is installed on the turntable 6, and a first mounting groove 13 for mounting the culture plate 16 is provided on the top of the adapter 12, and an adjustment hole 14 for mounting an adjustment screw is provided on the side wall of the first mounting groove 13.

[0067] Specifically, the adapter 12 is provided with a second mounting groove on the side opposite to the first mounting groove 13, and the second mounting groove is provided with protrusions corresponding to the number and position of the positioning grooves 61, and the adapter 12 is sleeved on the outer side of the turntable 6 through the second mounting groove, and the protrusions are embedded in the positioning grooves 61 of the turntable 6 to drive the adapter 12 and the turntable 6 to rotate synchronously. Therefore, the adapter 12 and the test tube disk 7 are installed in the same manner as the turntable 6, and they can be disassembled and assembled with the turntable 6 by simply plugging and unplugging along the axial direction.

[0068] A positioning block is provided on the inner wall of the first mounting groove 13 for limiting the position of the culture plate 16, and a material removal port is provided on the top of the adapter 12 for conveniently removing the culture plate 16 from the first mounting groove 13; the adjusting screw and the adjusting hole 14 are threadedly connected. After the culture plate 16 is installed in the first mounting groove 13, the adjusting screw is rotated, and its end will extend and retract along the axial direction to rest against the outer surface of the culture plate 16, ensuring that the culture plate 16 will not shake in the first mounting groove 13.

[0069] The adjustment holes 14 are provided on two adjacent sides of the first mounting groove 13 . After the adapter 12 is mounted on the turntable 6 , the adapter 12 can be compatible with most culture plates 16 through the cooperation between the adjustment screws and the adjustment holes 14 .

[0070] Working principle:

[0071] Connect the automatic sample introduction mechanism to the matching flow cytometer, and initialize the turntable 6 after power is turned on. After the system self-checks, confirm that all parameters have been set and the turntable 6 is in the test state;

[0072] Open the cover 83, take out the test tube tray 7, place the flow tubes to be tested, with a maximum of 40 tubes placed, then place the test tube tray 7 on the turntable 6 and close the cover 83;

[0073] The automatic sampling device operates automatically, and the stepper motor in the translation component 3 drives the translation plate 2 and the rotating component 4 to the specified position through the belt transmission method, and the precise position of the movement is controlled by the optical coupler;

[0074] The stepper motor in the rotating assembly 4 rotates the test tube tray 7 via a belt drive, placing the test tube at position 1 directly below the sampling needle 94. The mixer 56 rises to mix the test tube at position 1. After mixing, the sampling needle 94 moves downward to aspirate the sample.

[0075] After the test tube at position 1 is tested, the rotating assembly 4 rotates to align the next test tube with the sampling needle 94, and the above action is repeated until all 40 test tubes are tested. The translation assembly 3 returns the test tube tray 7 to the initial position.

[0076] Open the cover 83, take out the test tube tray 7, replace the test tubes, and proceed to the next round of testing.

[0077] If the test tube tray 7 is replaced with an adapter 12, it is compatible with various 96-well culture plates 16 and operates in the same manner as above.

[0078] In summary, the advantages of the present invention are: it can reduce manual participation and avoid errors; it has high movement accuracy and good repeatability; it is compatible with 5ml flow tubes and different models of 96-well culture plates 16, and has good applicability;

[0079] The entire turntable 6 is automatically operated by the cytometer host and is easy to use.

[0080] The operator places the test tube tray 7 into the instrument and clicks to start collection. No subsequent manual intervention is required, the operation is simple and easy to use, and the customer experience is good; the mechanical operation accuracy and efficiency are higher than manual operation, which reduces labor intensity and speeds up the test speed; common flow tubes and 96 deep-well plates and 96 shallow-well plates (round bottom, V-bottom, flat bottom) on the market are compatible, and consumables are easy to obtain.

[0081] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0082] In the description of this utility model, it should be noted that, unless otherwise expressly specified or limited, the terms "installed," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections, electrical connections; direct connections, indirect connections through an intermediate medium, and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model based on specific circumstances.

[0083] The above description is based on the ideal embodiment of the present invention. Based on the above description, relevant personnel can make various changes and modifications without departing from the technical scope of the present invention. The technical scope of the present invention is not limited to the content of the specification, but must be determined according to the scope of the claims.

Claims

1. A flow cytometer automatic sample introduction mechanism, comprising a bottom plate (1), characterized in that: The bottom plate (1) is connected to a horizontally movable translation plate (2) via a translation assembly (3), and the translation plate (2) is connected to a turntable (6) capable of rotating around its own axis via a rotation assembly (4). A loading component for clamping a test tube is installed on the turntable (6), and a mixing assembly (5) for mixing the sample in the test tube is also provided on the bottom plate (1).

2. The automatic sample introduction mechanism for flow cytometer according to claim 1, characterized in that: The translation assembly (3) comprises two slide rails (31) arranged opposite to each other, and a slider (32) is slidably mounted on each of the two slide rails (31). The two sliders (32) are fixedly connected via a connecting plate (33), and the translation plate (2) is fixedly mounted on the connecting plate (33). A linear module (34) parallel to the slide rail (31) is provided between the two sliders (32), and an output end of the linear module (34) is connected to the connecting plate (33).

3. The automatic sample introduction mechanism for flow cytometer according to claim 2, characterized in that: The rotating assembly (4) comprises a support plate (41) and a rotating shaft (43), wherein the rotating shaft (43) is rotatably arranged on the support plate (41), and the support plate (41) is fixedly connected to the translation plate (2) via a support column (42); A first motor (44) is also mounted on the translation plate (2). The output end of the first motor (44) is connected to a driving wheel (46). A driven wheel (45) is mounted on the lower end of the rotating shaft (43). A synchronous belt (47) is provided on the outer sides of the driving wheel (46) and the driven wheel (45). The upper end of the rotating shaft (43) is fixedly connected to the turntable (6).

4. The automatic sample introduction mechanism for flow cytometer according to claim 3, characterized in that: The mixing assembly (5) comprises a first guide rail (55) perpendicular to the bottom plate (1), a mixer (56) capable of being raised and lowered is slidably mounted on the first guide rail (55), and the top end of the mixer (56) is rotatable and has a groove corresponding to the bottom contour of the test tube.

5. The automatic sample introduction mechanism for flow cytometer according to claim 4, characterized in that: The mixing assembly (5) further comprises a second motor (51) mounted on the base plate (1); an output end of the second motor (51) is connected to a screw rod (52) parallel to the first guide rail (55); a nut (53) is screwed onto the screw rod (52); a connecting frame (54) is mounted on the nut (53); and a distal end of the connecting frame (54) is connected to the mixer (56).

6. The automatic sample introduction mechanism for flow cytometer according to claim 5, characterized in that: The material loading component adopts a test tube tray (7), and the lower part of the test tube tray (7) is sleeved on the turntable (6).

7. The automatic sample introduction mechanism for flow cytometer according to claim 6, characterized in that: A plurality of test tube grooves (71) are provided on the outer circumferential surface of the test tube plate (7), a bottom hole (72) is provided at the bottom of each test tube groove (71), and the bottom of the test tube can pass through the bottom hole (72) and contact the top groove of the mixer (56).

8. The automatic sample introduction mechanism for flow cytometer according to claim 7, characterized in that: The outer side of the turntable (6) is provided with an inwardly concave positioning groove (61), and the lower part of the test tube tray (7) is provided with positioning portions (75) corresponding to the position and number of the positioning grooves (61), and the positioning portions (75) are embedded in the positioning grooves (61).

9. The automatic sample introduction mechanism for flow cytometer according to claim 5, characterized in that: An adapter (12) is installed on the turntable (6), and the loading component adopts a culture plate (16); a first mounting groove (13) for mounting the culture plate (16) is provided on the top of the adapter (12), and an adjustment hole (14) for mounting an adjustment screw is provided on the side wall of the first mounting groove (13).

10. The automatic sample introduction mechanism for a flow cytometer according to any one of claims 1 to 9, characterized in that: A shell (81) is provided above the base plate (1), the translation assembly (3) and the rotation assembly (4) are both located in the shell (81), and the turntable (6) and the loading component are located above the shell (81); a support block (82) is provided on one side of the shell (81), a cover body (83) capable of being flipped longitudinally is installed on the top of the support block (82), and the cover body (83) and the support block (82) are connected by a hinge.

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