Hydroponic system for biocontrol bacterium root colonization test

Abstract

The utility model relates to the field of biocontrol strain research, in particular to a biocontrol bacterium root colonization test hydroponic system, which comprises a hydroponic tank, a hydroponic plate for fixing tobacco seedlings is horizontally erected inside the hydroponic tank, a connecting pipe is communicated with a water outlet end of the hydroponic tank, a manual stirrer is further arranged inside the hydroponic tank, and the connecting pipe is communicated with a water outlet end of the hydroponic tank. The stirrer comprises a swing rod, one end of the swing rod is bent, the swing rod vertically penetrates through the hydroponic tank, and the end, located in the hydroponic tank, of the swing rod is connected with a stirring rod. The impact influence on the cenobium attached to the root system is reduced as much as possible.

Description

Technical Field

[0001] The utility model relates to the research field of biocontrol strains, in particular to a hydroponic system for biocontrol fungus root colonization experiments. Background Art

[0002] Tobacco Fusarium root rot is a major rhizome disease in my country's tobacco-producing regions. Current chemical fungicides can pollute the environment and contribute to the development of drug resistance. However, biological control using biocontrol agents with antagonistic effects against Fusarium spp. is highly effective, pollution-free, and residue-free, and will become a key approach to tobacco rhizome disease control in the future.

[0003] Therefore, it is possible to isolate, screen, and identify highly effective antagonists of tobacco Fusarium root rot from different plants and habitats. Furthermore, beneficial biocides that can promote the colonization of biocontrol strains can be identified from tobacco root exudates. Their effects on the growth, chemotaxis, and root film-forming ability of biocontrol bacteria can be evaluated, and their metabolites can be analyzed and identified. Finally, these can be used as prebiotics, combined with biocontrol strains, to precisely design rhizospheric synbiotics, modulating the mutualistic relationship between rhizospheric biocontrol microorganisms and tobacco plants, thereby reducing the incidence of root rot.

[0004] Currently, soil or hydroponic culture experiments are commonly used to study biocontrol fungi colonization on roots. Hydroponic culture offers the advantage of easier sampling compared to soil culture, but existing hydroponic systems present certain inconveniences when applying these tests. Because different prebiotics must be added to determine their effects on biocontrol fungi colonization, the hydroponic liquid must be stirred after adding the prebiotics to ensure uniform distribution. However, existing stirring devices have a large output motion, which can easily dislodge bacterial clumps from the roots. Manual stirring with a glass rod also limits operating space, as tobacco seedling roots grow erratically, and circular stirring with the rod can easily touch the roots. Utility Model Content

[0005] The purpose of the present invention is to provide a hydroponic system for the test of biocontrol fungi root colonization in order to solve the above-mentioned problems. The present invention adopts a manually driven stirring rod that can move up and down to slowly stir the inside of the hydroponic box, thereby minimizing the impact on the bacterial masses that have already attached to the roots while ensuring the uniform distribution of beneficial biomass.

[0006] The utility model achieves the above-mentioned purpose through the following technical solutions: a hydroponic system for biocontrol fungi root colonization test, comprising a hydroponic box with a hydroponic plate for fixing tobacco seedlings horizontally arranged inside, the hydroponic box being connected to a connecting pipe at the water outlet end, and a hand-adjustable stirrer being further arranged inside the hydroponic box, the stirrer comprising a swing rod with one end bent and vertically passing through the hydroponic box, the swing rod being located at the end of the hydroponic box and connected to a stirring rod.

[0007] Preferably, an observation window closed by a transparent plate is provided on one side of the hydroponic box near the corner, and a vertically extending slide rail is provided on the side of the observation window. A slide seat is provided on the slide rail, and the stirring rod is arranged near the inner wall of the hydroponic box and the end thereof is fixedly connected to the slide seat.

[0008] Preferably, the outer sliding sleeve of the rocker arm is provided with a sliding sleeve, and the sliding sleeve is connected to the sliding seat via a ball-joint universal coupling.

[0009] Preferably, the bent portion of the swing rod is assembled with a preset waterproof bearing of the hydroponic box, and a hand plate is provided at the end of the swing rod located outside.

[0010] Preferably, the hydroponic box is further provided with an air inlet pipe, and an air outlet end of the air inlet pipe located at the bottom end of the hydroponic box is provided with an air bubble stone.

[0011] Preferably, a buffer sponge belt is provided inside the hydroponic box just above the air stone. As a preferred solution, when the oxygen content in the water inside the hydroponic box needs to be increased, the air pump is turned on to introduce air into the hydroponic box through the air inlet pipe and the air stone. At the same time, in order to prevent bubbles from rising violently and hitting the roots of the tobacco seedlings, the present invention provides a buffer sponge belt to block the rising bubbles, thereby slowing them down.

[0012] The working process of this utility model is as follows:

[0013] After adding beneficial biomass into the hydroponic tank, the staff can slowly operate the rotating handwheel to rotate it repeatedly in the clockwise and counterclockwise directions. The handwheel drives the pendulum rod to rotate synchronously, and the sliding sleeve on the pendulum rod moves back and forth along the pendulum rod and drives the stirring rod to move up and down through the sliding seat.

[0014] At the same time, the staff can observe the position of the tobacco seedling roots in the hydroponic box through the observation window, and then adjust the height of the upward stroke of the stirring rod to prevent the stirring rod from touching the tobacco seedling roots.

[0015] The beneficial effect of the utility model is that the utility model adopts a manually driven stirring rod that can move up and down to slowly stir the inside of the hydroponic box, while ensuring the uniform distribution of beneficial biomass and minimizing the impact on the bacterial mass that has already attached to the root system. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural schematic diagram of the hydroponic system described in the specific implementation method;

[0017] Figure 2 Schematic diagram of the internal structure of the hydroponic system according to the specific embodiment;

[0018] Figure 3Schematic diagram of the connection relationship between the rocker arm and the slide rail according to the specific implementation method. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention. Example 1

[0020] like Figure 1-3 As shown, a hydroponic system for a biocontrol fungus root colonization test comprises a hydroponic box 1 with a hydroponic plate 2 for fixing tobacco seedlings horizontally mounted inside, the hydroponic box 1 being connected to a connecting pipe 4 at the water outlet end, a hand-adjustable stirrer being further provided inside the hydroponic box 1, the stirrer comprising a bent end assembled with a waterproof bearing 7 preset on the hydroponic box 1, and a pendulum 8 located at the end outside thereof being provided with a hand plate 5 penetrating the pendulum 8 of the hydroponic box 1, the pendulum 8 being located at the end of the hydroponic box 1 and the sliding sleeve being provided with a sliding sleeve 11, the sliding sleeve 11 being connected to a sliding seat 10 via a ball-hinged universal coupling.

[0021] The hydroponic box 1 is provided with an observation window 12 closed by a transparent plate on one side near the corner. The hydroponic box 1 is provided with a vertically extending slide rail 9 next to the observation window 12. The slide rail 9 is slidably engaged with the slide seat 10. The stirring rod 13 is arranged near the inner wall of the hydroponic box and the end thereof is fixedly connected to the slide seat 10.

[0022] After adding beneficial biomass into the hydroponic box, the staff can slowly operate the rotating hand plate 5 to rotate it repeatedly in the clockwise and counterclockwise directions. The hand plate 5 drives the swing rod 8 to rotate synchronously, and the sliding sleeve 11 on the swing rod 8 moves back and forth along the swing rod 8 and drives the stirring rod 13 to move up and down reciprocatingly through the sliding seat 10.

[0023] At the same time, the staff can observe the position of the tobacco seedling roots in the hydroponic box through the observation window 12, and then adjust the height of the upward stroke of the stirring rod 13 to prevent the stirring rod 13 from touching the tobacco seedling roots. Example 2

[0024] like Figure 1-3 As shown, the difference between this embodiment and embodiment 1 is that: the hydroponic box 1 is also provided with an air inlet pipe 3, and the air inlet pipe 3 is located at the air outlet end of the bottom end of the hydroponic box 1 and is provided with an air bubble stone 6, and the hydroponic box 1 is provided with a buffer sponge belt 61 just above the air bubble stone 6.

[0025] When it is necessary to increase the water oxygen content inside the hydroponic box, the air pump is turned on to introduce air into the hydroponic box through the air inlet pipe 3 and the bubble stone 6. At the same time, in order to prevent the bubbles from rising violently and hitting the roots of the tobacco seedlings, the utility model is provided with a buffer sponge belt 61 to block the rising bubbles and slow down the bubbles.

[0026] Specifically, the biocontrol bacteria and beneficial biomass used in the present invention are obtained by collecting tobacco root rot-infected tobacco plants, healthy tobacco plants, and their rhizosphere soil from tobacco fields in Luohe, and isolating and purifying microbial strains from special habitats such as Zhangye Desert Park and deserts, saline-alkali land, wetlands, Hainan tropical rainforests, Dalian oceans, volcanic rocks in Inner Mongolia, and Tengchong hot springs in Yunnan. The strains are then classified and identified through physiological and biochemical trait testing, morphological observation, and molecular sequencing.

[0027] Through indoor plate confrontation experiments, we preliminarily screened the biocontrol strains with good antagonistic effects on tobacco Fusarium root rot pathogens, and put them into further pot experiments and field rescreening to screen out the microbial strains with good control effects on tobacco root rot and preserve them;

[0028] In view of the highly effective antagonistic bacteria against tobacco Fusarium root rot isolated in the above research, we first analyzed and compared the different components in the root secretions of tobacco plants inoculated with pathogens and the control, and then screened out the key components that can promote the rhizosphere colonization of biocontrol bacteria.

[0029] First, the metabolite composition of root exudates was determined using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, and the differences in the metabolome of root exudates between tobacco plants inoculated with pathogens and the control were analyzed and compared.

[0030] Then, different components were added exogenously in a sterile root-dividing hydroponic system to study their effects on the colonization ability of biocontrol bacteria on tobacco root surfaces.

[0031] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A biocontrol fungus root colonization test hydroponic system, comprising a hydroponic box (1) with a hydroponic plate (2) for fixing tobacco seedlings erected horizontally therein, the hydroponic box (1) being connected to a connecting pipe (4) at a water outlet end, characterized in that: A hand-adjustable stirrer is further provided inside the hydroponic box (1), and the stirrer comprises a swing rod (8) with one end bent and vertically penetrating the hydroponic box (1). The swing rod (8) is located at the end of the hydroponic box (1) and is connected to a stirring rod (13).

2. The biocontrol fungus root colonization test hydroponic system according to claim 1, characterized in that: The hydroponic box (1) is provided with an observation window (12) enclosed by a transparent plate on one side near the corner. The hydroponic box (1) is provided with a vertically extending slide rail (9) beside the observation window (12). A slide seat (10) is slidably provided on the slide rail (9). The stirring rod (13) is provided near the inner wall of the hydroponic box and its end is fixedly connected to the slide seat (10).

3. The biocontrol fungus root colonization test hydroponic system according to claim 2, characterized in that: The outer sliding sleeve of the rocker arm (8) is provided with a sliding sleeve (11), and the sliding sleeve (11) is connected to the sliding seat (10) via a ball-jointed universal joint.

4. The biocontrol bacteria root colonization test hydroponic system according to claim 2, characterized in that: The bent portion of the swing rod (8) is assembled with a preset waterproof bearing (7) of the hydroponic box (1), and a hand plate (5) is provided at one end of the swing rod (8) located outside.

5. The biocontrol fungus root colonization test hydroponic system according to claim 1, characterized in that: The hydroponic box (1) is further provided with an air inlet pipe (3), and an air outlet end of the air inlet pipe (3) located at the bottom end inside the hydroponic box (1) is provided with an air bubble stone (6).

6. The biocontrol bacteria root colonization test hydroponic system according to claim 5, characterized in that: A buffer sponge belt (61) is provided inside the hydroponic box (1) just above the air stone (6).

Images