Refrigeration temperature control type ultrasonic cell crusher

Abstract

A refrigeration temperature control type ultrasonic cell disruption instrument relates to the technical field of biological cell treatment and comprises a box body, the top of the box body is provided with a downward and inward extending disruption probe, and a liftable support plate is arranged below the disruption probe; the box body is divided into a crushing chamber and an element chamber by a partition plate arranged in the box body; the crushing probe and the supporting plate are positioned in the crushing chamber; a refrigeration system is also arranged in the box body and comprises a semiconductor refrigeration sheet which is embedded in the partition plate, the refrigeration surface faces the crushing chamber, and the heat dissipation surface faces the element chamber; the heat dissipation assembly comprises a heat dissipation device in contact connection with the heat dissipation surface of the semiconductor refrigeration sheet and a first heat dissipation fan fixed below the heat dissipation device; the refrigeration assembly comprises a water storage tank, a water pump and a refrigeration water tank which are sequentially and circularly connected, the water storage tank and the water pump are arranged in the element chamber, and the refrigeration water tank is in contact connection with the refrigeration surface of the semiconductor refrigeration sheet and located in the crushing chamber; and the second cooling fan is fixed on the side wall of the box body in the crushing chamber. To-be-crushed cells are refrigerated through the refrigerating system, and in addition, a heat insulation layer is arranged on the side wall of the crushing chamber for cold insulation, so that the refrigerating time is prolonged, and an experiment is more convenient to carry out.

Description

Technical Field

[0001] The utility model relates to the technical field of biological cell processing, in particular to a refrigeration temperature-controlled ultrasonic cell disruptor. Background Art

[0002] Ultrasonic cell disruptor converts electrical energy into sound energy through a transducer. This energy passes through the liquid medium and turns into dense small bubbles. These small bubbles explode rapidly, generating energy like small bombs, thereby breaking up cells and other substances.

[0003] Ultrasonic cell disruption releases heat, which can denature intracellular proteins and other substances (mitochondria, chloroplasts, etc.), thus affecting subsequent experiments. Most existing ultrasonic cell disruptors require their own ice box to prevent denaturation of cellular contents due to overheating during cell disruption. Utility Model Content

[0004] In view of the problems and shortcomings in the prior art, the utility model provides a refrigeration temperature-controlled ultrasonic cell disruptor.

[0005] The technical solution of this utility model is as follows:

[0006] A refrigerated temperature-controlled ultrasonic cell disruptor comprises a housing, a top portion of which is provided with a disruption probe extending downward and inward, a lower portion of which is provided with a liftable support plate for placing a beaker containing a liquid containing cells to be disrupted;

[0007] The box is divided into a crushing chamber and a component chamber arranged in parallel above and below by a partition arranged inside the box. The crushing probe and the support plate are located in the crushing chamber. Specifically, the support plate is fixed to the bottom of the crushing chamber by a lifting mechanism.

[0008] The box is also equipped with a refrigeration system, including:

[0009] The semiconductor refrigeration chip is embedded in the partition, with the cooling surface facing the crushing chamber and the heat dissipation surface facing the component chamber;

[0010] The heat dissipation assembly includes a radiator in contact with the heat dissipation surface of the semiconductor refrigeration plate and a first heat dissipation fan fixed below the radiator;

[0011] The refrigeration assembly includes a water storage tank, a water pump and a refrigeration water tank which are connected in a circular manner in sequence. The water storage tank and the water pump are arranged in the component room. The refrigeration water tank is in contact with the refrigeration surface of the semiconductor refrigeration plate and is located in the crushing room.

[0012] The second heat dissipation fan is fixed on the side wall of the box body in the crushing chamber and is located above the refrigeration water tank.

[0013] According to a specific embodiment, the outer cover of the crushing probe is provided with a metal heat conductive sheet. Furthermore, the metal heat conductive sheet is cylindrical and extends downward from the top of the crushing probe, with the bottom surface 40-60 mm away from the lower end of the crushing probe. This metal heat conductive sheet is used to conduct heat to the crushing probe, thereby reducing the temperature of the crushing probe.

[0014] According to a specific embodiment, the cooling water tank is made of a heat-conducting metal material, preferably aluminum or copper. The thickness of the partition is greater than that of the semiconductor cooling fin, and the heat dissipation surface of the semiconductor cooling fin is flush with the bottom surface of the partition. An aluminum heat conduction plate is provided between the heat dissipation surface and the cooling water tank.

[0015] In order to improve the cooling effect in the crushing chamber and reduce the heat exchange between the box and the outside, an insulation layer is provided on the inner wall of the crushing chamber. Furthermore, the insulation layer is made of foam or sponge.

[0016] To monitor the temperature inside the crushing chamber and adjust it in real time, a temperature sensor is installed on the support plate. Specifically, the temperature sensor, water pump, and first and second cooling fans are all connected to a control unit. The temperature sensor transmits temperature information to the control unit, which compares it with the set temperature and issues control commands to start and stop the water pump and the first and second cooling fans.

[0017] The beneficial effects of the utility model are:

[0018] 1) A refrigeration system is installed inside the box. The refrigeration system includes a semiconductor refrigeration plate, a heat dissipation component fixed below the semiconductor refrigeration plate, a refrigeration component fixed above the semiconductor refrigeration plate, and a second heat dissipation fan installed in the crushing chamber to cool the cells to be crushed. In addition, an insulation layer is installed on the side wall of the crushing chamber to keep the cells cold, extend the refrigeration time, and make the experiment more convenient.

[0019] 2) The probe is directly cooled by the heat-conducting metal sheet surrounding the probe, which can effectively maintain the temperature inside the crushing container and prevent the denaturation of cell contents due to overheating during cell disruption. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a cross-sectional view of an embodiment;

[0021] Figure 2 It is the connection diagram of the refrigeration components;

[0022] 1. Box body; 11. Crushing chamber; 12. Component chamber; 2. Partition; 3. Crushing probe; 4. Refrigeration system; 5. Second cooling fan; 6. Metal heat conductive plate; 7. Support plate; 41. Semiconductor cooling plate; 42. Water tank; 43. Radiator; 44. First cooling fan; 45. Water tank; 46. Water pump. DETAILED DESCRIPTION

[0023] The following will further illustrate the technical means adopted to achieve the predetermined inventive purpose of the present invention in conjunction with the drawings in the embodiments of the present invention.

[0024] See also Figure 1 and Figure 2 As shown, Figure 1 is a cross-sectional view of an embodiment; Figure 2 This is a connection diagram of the refrigeration components.

[0025] A refrigerated temperature-controlled ultrasonic cell disruptor comprises a housing 1, a top portion of which is provided with a disruption probe 3 extending downward and inward, a lower portion of which is provided with a liftable support plate 7 for placing a beaker containing a liquid containing cells to be disrupted;

[0026] The housing 1 is divided into a crushing chamber 11 and a component chamber 12 arranged in parallel above and below by a partition 2 provided therein. The crushing probe 3 and the support plate 7 are located in the crushing chamber 11. Specifically, the support plate 7 is fixed to the bottom of the crushing chamber 11 by a lifting mechanism.

[0027] The box 1 is also provided with a refrigeration system 4, including:

[0028] The semiconductor cooling sheet 41 is embedded in the partition 2, with the cooling surface facing the crushing chamber 11 and the heat dissipation surface facing the element chamber 12;

[0029] The heat dissipation component includes a heat sink 43 in contact with the heat dissipation surface of the semiconductor refrigeration plate 41 and a first heat dissipation fan 44 fixed below the heat sink 43;

[0030] The refrigeration assembly includes a water storage tank 45, a water pump 46, and a refrigeration water tank 42 that are cyclically connected in sequence. The water storage tank 45 and the water pump 46 are arranged in the element chamber 12. The refrigeration water tank 42 is in contact with the refrigeration surface of the semiconductor refrigeration plate 41 and is located in the crushing chamber 11.

[0031] The second cooling fan 5 is fixed on the side wall of the box body 1 in the crushing chamber 11 and is located above the refrigeration water tank 42.

[0032] According to a specific embodiment, the crushing probe 3 is provided with a metal heat conductive sheet 6. Furthermore, the metal heat conductive sheet 6 is cylindrical and extends downward from the top of the crushing probe 3, with the bottom surface 40-60 mm away from the lower end of the crushing probe 3. It is used to conduct heat to the crushing probe 3, thereby reducing the temperature of the crushing probe 3.

[0033] According to a specific embodiment, the cooling water tank 42 is made of a heat-conducting metal material, preferably aluminum or copper. The thickness of the partition 2 is greater than that of the semiconductor cooling plate 41, and the heat dissipation surface of the semiconductor cooling plate 41 is flush with the bottom surface of the partition 2. An aluminum heat conduction plate is provided between the heat dissipation surface and the cooling water tank 42.

[0034] In order to improve the cooling effect in the crushing chamber 11 and reduce the heat exchange between the box body 1 and the outside, an insulation layer is provided on the inner wall of the crushing chamber 11. Furthermore, the material of the insulation layer is foam or sponge.

[0035] To detect the temperature within the crushing chamber 11 and adjust it in real time, a temperature sensor is provided on the support plate 7. Specifically, the temperature sensor, water pump 46, and first and second cooling fans 44 and 5 are all connected to a control unit. The temperature sensor transmits temperature information to the control unit, which compares it with the set temperature and issues control instructions to start and stop the water pump 46 and the first and second cooling fans 44 and 5.

[0036] The above is a preferred embodiment of the present invention, but the present invention is not limited to the above embodiments and examples. Various changes, equivalent substitutions, improvements, etc. made within the scope of knowledge possessed by those skilled in the art without departing from the concept of the present invention should be included in the scope of protection of the present invention.

Claims

1. A refrigerated temperature-controlled ultrasonic cell disruptor, comprising a housing (1), a top portion of which is provided with a disruption probe (3) extending downward and inward, and a liftable support plate (7) provided below the disruption probe; It is characterized by: The box body (1) is divided into a crushing chamber (11) and an element chamber (12) arranged in parallel up and down by a partition (2) arranged therein, and the crushing probe (3) and the supporting plate (7) are located in the crushing chamber (11); A refrigeration system (4) is also provided in the box (1), comprising: A semiconductor refrigeration sheet (41) is embedded in the partition (2), with a cooling surface facing the crushing chamber (11) and a heat dissipation surface facing the element chamber (12); A heat dissipation component comprises a radiator (43) in contact with a heat dissipation surface of a semiconductor refrigeration plate (41) and a first heat dissipation fan (44) fixed below the radiator (43); A refrigeration assembly comprises a water storage tank (45), a water pump (46) and a refrigeration water tank (42) which are cyclically connected in sequence, wherein the water storage tank (45) and the water pump (46) are arranged in the element chamber (12), and the refrigeration water tank (42) is in contact with the refrigeration surface of the semiconductor refrigeration plate (41) and is located in the crushing chamber (11); The second heat dissipation fan (5) is fixed on the side wall of the box body (1) in the crushing chamber (11) and is located above the refrigeration water tank (42).

2. The cell disruptor according to claim 1, characterized in that The outer shell of the crushing probe (3) is provided with a metal heat conducting sheet (6).

3. The cell disruptor according to claim 2, characterized in that The metal heat conducting sheet (6) is cylindrical and extends downward from the top of the crushing probe (3), with the bottom surface being 40-60 mm away from the lower end of the crushing probe (3).

4. The cell disruptor according to any one of claims 1 to 3, characterized in that The refrigeration water tank (42) is made of a metal heat-conducting material.

5. The cell disruptor according to claim 4, characterized in that The thickness of the partition (2) is greater than that of the semiconductor refrigeration plate (41), and the heat dissipation surface of the semiconductor refrigeration plate (41) is flush with the bottom surface of the partition (2), and an aluminum heat conduction plate is provided between the heat dissipation surface and the refrigeration water tank (42).

6. The cell disruptor according to claim 5, characterized in that A heat insulating layer is provided on the inner side wall of the crushing chamber (11).

7. The cell disruptor according to claim 6, characterized in that The material of the heat insulating layer is foam or sponge.

8. The cell disruptor according to claim 7, characterized in that A temperature sensor is provided on the supporting plate (7).

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