Tissue cleaning and disinfecting device

Abstract

The utility model provides a tissue cleaning and disinfecting device which comprises a cleaning pool, a tissue inlet pipe communicated with the top of the cleaning pool, a liquid inlet pipe and a containing groove communicated with the bottom of the cleaning pool, and a tissue outlet pipe and a liquid outlet pipe are arranged at the bottom of the containing groove; a cleaning bearing piece with water leakage holes is arranged in the cleaning pool. An oscillator is arranged on the outer side of the containing groove, a baffle is movably arranged at the joint of the containing groove and the tissue outlet pipe, the baffle movably blocks a bottom outlet of the containing groove, and thorough cleaning or disinfection can be guaranteed through the arrangement of the oscillator. Meanwhile, due to the arrangement of the cleaning filter screen, the tissue blocks can be smoothly discharged without the help of external instruments under the condition that the tissue cleaning and disinfecting device is not opened. According to the device, the cleaning or disinfecting efficiency is improved, different tissue blocks can be cleaned and disinfected without being placed in a biological safety cabinet or an ultra-clean workbench, sterile primary cells can be conveniently separated out through the tissue blocks, and the success rate of primary culture is guaranteed.

Description

Technical Field

[0001] The utility model belongs to the technical field of biological equipment and relates to a tissue cleaning and disinfecting device. Background Art

[0002] Primary cell culture is the first in vitro culture of tissue cells obtained from a donor. Cells obtained from primary cultures have diploid genetics and can be used as a research tool, with applications in drug sensitivity testing, cell differentiation, cell therapy, gene function, and regulatory mechanisms.

[0003] When culturing primary cells, it is necessary to obtain target tissue samples from animals or obtain samples from human waste tissues for in vitro culture. Samples from different sources are subject to significant differences in microbial contamination, and there are many types of contaminated microorganisms. Most samples are usually taken in a contaminated state. Although strict instrument disinfection and aseptic operation can help reduce the probability of microbial contamination, they cannot completely avoid microbial contamination. However, primary cultured cells can only grow well in a sterile environment. Therefore, in order to successfully perform primary in vitro cell culture, it is usually necessary to clean the target tissue and then disinfect it.

[0004] Currently, tissues are typically cleaned or disinfected after collection by technicians using forceps to shake the tissue block back and forth in a cleaning / disinfectant solution, or by periodically turning the tissue with sterile forceps in the cleaning / disinfectant solution. However, manual tissue cleaning / disinfection requires a biosafety cabinet or clean bench, which is not only time-consuming and labor-intensive, but also increases staff workload, significantly impacting experimental efficiency. Utility Model Content

[0005] The purpose of the utility model is to provide a tissue cleaning and disinfecting device to solve the problem that the existing tissue cleaning or disinfecting methods are time-consuming and labor-intensive.

[0006] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0007] The present application provides a tissue cleaning and disinfection device, comprising a cleaning pool, a tissue inlet pipe and a liquid inlet pipe connected to the top of the cleaning pool, and a receiving tank connected to the bottom of the cleaning pool, wherein the bottom of the receiving tank is respectively provided with a tissue outlet pipe and a liquid outlet pipe; a cleaning support member with a water leakage hole is provided inside the cleaning pool; an oscillator is provided on the outside of the receiving tank, and a baffle is movably provided at the connection between the receiving tank and the tissue outlet pipe, and the baffle movably blocks the bottom outlet of the receiving tank.

[0008] Preferably, the cleaning support is connected to a filter push rod via a connecting rod, and the filter push rod is located outside the cleaning tank.

[0009] Preferably, the cleaning support member includes two fixed rods arranged on the inner wall of the cleaning pool; each of the fixed rods is provided with two fixed plates, a cleaning filter is arranged between the two fixed plates, the fixed plates are movably connected to the filter push rod through the connecting rod, and the filter push rod drives the two cleaning filters to rotate relative to each other.

[0010] Preferably, two positioning filters are vertically provided inside the accommodating tank, the two positioning filters are respectively located at the top of the tissue outlet tube, and the distance between the two positioning filters is less than or equal to the diameter or width of the tissue outlet tube.

[0011] Preferably, the baffle is connected to a baffle pushing rod.

[0012] Preferably, a laminar flow hood and an ultraviolet lamp are provided above the cleaning pool, and the ultraviolet lamp is located on one side of the laminar flow hood.

[0013] Preferably, a tissue inlet cover is provided on the top of the tissue inlet tube, and a liquid inlet cover is provided on the top of the liquid inlet tube.

[0014] Preferably, a plurality of rubber connecting seats are provided at the bottom of the cleaning tank.

[0015] Preferably, the tissue cleaning and disinfection device also includes a shell and a partition horizontally arranged inside the shell, the cleaning pool is located on the partition, and the inlet of the tissue inlet pipe, the inlet of the liquid inlet pipe, the outlet of the tissue outlet pipe and the outlet of the liquid outlet pipe are all exposed to the shell.

[0016] The utility model has the following beneficial effects:

[0017] (1) The setting of two cleaning filters enables the tissue blocks to be discharged smoothly without the help of external instruments without opening the tissue cleaning and disinfection device, thereby obtaining sterile tissue blocks and avoiding the risk of re-contamination of the tissue blocks.

[0018] (2) The mesh diameter of the cleaning filter can be set to different sizes to suit tissue blocks of different sizes, while also preventing the loss of smaller tissue blocks.

[0019] (3) The setting of the oscillator can keep the tissue block in a non-static state, ensuring that all parts of the tissue block can fully contact with the cleaning solution or disinfectant to ensure thorough cleaning or disinfection.

[0020] (4) The bottom of the storage tank is set in an inclined shape to completely drain the waste liquid and reduce the residual waste liquid on the tissue block. The setting of the laminar flow hood can further reduce the residual waste liquid on the tissue block.

[0021] (5) The setting of the closed tissue outlet tube can realize the aseptic collection of tissue blocks and prevent the cleaned and disinfected tissue blocks from being contaminated again. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A schematic diagram of the three-dimensional structure of the tissue cleaning and disinfection device provided in an embodiment of the present application;

[0023] Figure 2 A schematic diagram of the rear perspective structure of the tissue cleaning and disinfection device provided in an embodiment of the present application;

[0024] Figure 3 A diagram showing the state of the cleaning filter when it is opened according to an embodiment of the present application;

[0025] Figure 4 A state diagram of the cleaning filter when it is merged provided in an embodiment of the present application;

[0026] Figure 5 Provided in the embodiments of this application Figure 1 Cross-sectional view in

[0027] Figure 6 Provided in the embodiments of this application Figure 5 Schematic diagram of the enlarged structure at A in the middle;

[0028] Figure 7 A diagram showing the external shape of the receiving groove provided in an embodiment of the present application;

[0029] Figure 8 A schematic diagram of the top perspective structure of the tissue cleaning and disinfection device provided in an embodiment of the present application;

[0030] Figure 9 A schematic diagram of the external structure of the tissue cleaning and disinfection device provided in an embodiment of the present application;

[0031] Figure 10 A front view of the tissue cleaning and disinfection device provided in an embodiment of the present application;

[0032] Symbols represent:

[0033] 1-cleaning tank, 2-tissue inlet pipe, 3-liquid inlet pipe, 4-accommodating tank, 5-tissue outlet pipe, 6-liquid outlet pipe, 7-cleaning support, 8-oscillator, 9-baffle, 10-connecting rod, 11-filter push rod, 12-positioning filter, 13-baffle push rod, 14-laminar flow hood, 15-UV lamp, 16-tissue inlet cover, 17-liquid inlet cover, 18-rubber connector, 19-housing, 20-partition;

[0034] 701-fixing rod, 702-fixing plate, 703-cleaning filter. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0036] The tissue cleaning and disinfection device provided in the embodiment of the present application includes a cleaning pool 1, a tissue inlet pipe 2, a liquid inlet pipe 3, a receiving tank 4, a tissue outlet pipe 5, a liquid outlet pipe 6 and an oscillator 8, etc. Figure 1 shown.

[0037] Specifically, the cleaning tank 1 is the main component of the tissue cleaning and disinfection device provided in the embodiment of the present application, which is used to clean or disinfect tissue blocks. The cleaning tank 1 is a circular or square structure, and can be specifically set according to actual conditions. A cleaning support 7 is provided inside the cleaning tank 1, and the cleaning support 7 is used to place tissue blocks to be cleaned or disinfected. In order to prevent liquids such as cleaning liquid or disinfectant from remaining between the tissue blocks and the cleaning support 7, the cleaning support 7 is provided with a leakage hole. In order to discharge the cleaned or disinfected tissue blocks from the cleaning support 7 to the outside of the cleaning tank 1 under sterile conditions, the cleaning support 7 is connected to the filter push rod 11 through a connecting rod 10. The filter push rod 11 is located on the outside of the cleaning tank 1, as shown in the attached figure. Figure 2 shown.

[0038] Furthermore, the cleaning support member 7 in the embodiment of the present application is a symmetrically arranged structure, which includes two fixing rods 701, and the two ends of each fixing rod 701 are respectively fixed to the inner wall of the cleaning tank 1 to achieve the fixing of the cleaning support member 7. Two fixing plates 702 are respectively provided at the two ends of the fixing rod 701, and a cleaning filter 703 is provided between the two fixing plates 702. As a result, the cleaning support member 7 forms two symmetrically arranged U-shaped structures, as shown in the attached figure. Figure 3 、 4 A fixing plate 702 on each fixing rod 701 is connected to the end of a connecting rod 10, and the other end of the connecting rod 10 is commonly connected to the filter push rod 11, thereby achieving the connection between the cleaning support member 7 and the filter push rod 11.

[0039] In the embodiment of the present application, the end of the connecting rod 10 can rotate around the filter push rod 11, so that the filter push rod 11 can drive the cleaning filter 703 to open and merge. Specifically, the push rod of the filter push rod 11 moves up and down outside the cleaning tank 1, which can drive the connecting rod 10 to move up and down. Because the two ends of the connecting rod 10 are movably connected to the filter push rod 11 and the fixed plate 702, when the push rod of the filter push rod 11 moves upward, the connecting rod 10 moves upward, and the end of the connecting rod 10 rotates around the filter push rod 11. Because the two ends of the fixed rod 701 are fixed to the inner wall of the cleaning tank 1, the upward movement of the connecting rod 10 causes the connecting rod 10 and the fixed plate 702 to rotate, thereby driving the fixed plate 702 to move upward until the fixed plate 702 is horizontal, thereby merging the cleaning filter 703. The process of opening the cleaning filter 703 is the process of the push rod of the filter push rod 11 moving downward.

[0040] Furthermore, the filter push rod 11 is arranged on the outside of the cleaning tank 1. In order to realize the opening and merging of the cleaning filter 703 inside the cleaning tank 1, the push rod of the filter push rod 11 is a U-shaped structure. Figure 3 shown.

[0041] In the embodiment of the present application, the opening and merging of the two cleaning filters 703 enables the tissue blocks to be discharged smoothly without the aid of external instruments without opening the tissue cleaning and disinfection device, thereby obtaining sterile tissue blocks and avoiding the risk of re-contamination of the tissue blocks.

[0042] In the embodiment of the present application, the mesh diameters on the cleaning filter 703 can be set to different sizes so as to be suitable for tissue blocks of different volumes, while also preventing the loss of smaller tissue blocks.

[0043] Both the tissue inlet tube 2 and the liquid inlet tube 3 are disposed at the top of the cleaning tank 1 and are in communication with the cleaning tank 1, allowing tissue blocks to be cleaned and disinfected and liquids such as cleaning or disinfecting solutions to be discharged into the interior of the cleaning tank 1 through the tissue inlet tube 2 and the liquid inlet tube 3, respectively. Furthermore, to prevent foreign matter from falling into the cleaning tank 1, a tissue inlet cap 16 and a liquid inlet cap 17 are provided at the top of the tissue inlet tube 2 and the liquid inlet tube 3, respectively. In this embodiment of the present application, the tissue inlet tube 2 and the tissue inlet cap 16 are detachably connected by threads, and the liquid inlet tube 3 and the liquid inlet cap 17 are also detachably connected by threads.

[0044] The receiving tank 4 is arranged at the bottom of the cleaning tank 1 and is connected to the cleaning tank 1. When the tissue is cleaned or disinfected, the receiving tank 4 is used to hold the disinfectant or cleaning liquid. When the cleaning or disinfection is completed, the receiving tank 4 is used to directionally discharge the cleaned or disinfected tissue block. In addition, the oscillator 8 is arranged on the outer side wall of the receiving tank 4, specifically on the outer side wall of the receiving tank 4. The oscillation of the oscillator 8 can drive the receiving tank 4 to oscillate, and then drive the cleaning tank 1 to oscillate, and finally enable the various parts of the tissue block located inside the cleaning tank 1 to fully contact with the cleaning liquid or disinfectant, ensuring thorough cleaning or disinfection. In addition, in order to keep the cleaning tank 1 stable during the oscillation process, a plurality of rubber connecting seats 18 are provided at the bottom of the cleaning tank 1.

[0045] In the embodiment of the present application, in order to realize the directional discharge of tissue blocks in the receiving tank 4, two positioning filters 12 are vertically provided inside the receiving tank 4, as shown in the attached Figure 5 、 6 As shown. The two positioning filters 12 are respectively located at the top of the tissue outlet tube 5 and are arranged along the length direction of the tissue outlet tube 5. At the same time, the two positioning filters 12 are located between the two cleaning filters 703, so that after the two cleaning filters 703 are opened, the tissue blocks can fall between the two positioning filters 12 and then slide into the tissue outlet tube 5. Furthermore, the distance between the two positioning filters 12 is greater than the distance between the ends of the two cleaning filters 703 after they are opened, so that the tissue blocks can be smoothly caught and will not get stuck on the two positioning filters 12. In addition, the distance between the two positioning filters 12 is less than or equal to the diameter or width of the tissue outlet tube 5, so that the tissue blocks can smoothly enter the tissue outlet tube 5 and will not get stuck at the tissue outlet tube 5.

[0046] A tissue outlet tube 5 and a liquid outlet tube 6 are located at the bottom of the storage tank 4 and communicate with the storage tank 4. The tissue outlet tube 5 is also connected to a sterile collection device, and the liquid outlet tube 6 is also connected to a waste liquid collection bottle. After cleaning and disinfection, tissue blocks are discharged through the tissue outlet tube 5 into the sterile collection device for aseptic collection. Waste liquid generated after cleaning and disinfection is discharged through the liquid outlet tube 6 into the waste liquid collection bottle.

[0047] In the embodiment of the present application, the bottom of the accommodating tank 4 is arranged in an inclined form, and the liquid outlet pipe 6 is located at the lowest point of the inclined surface, as shown in the attached figure. Figure 7 As shown. The bottom surface of the inclined receiving tank 4 can completely drain the waste liquid and reduce the residual waste liquid on the tissue block. In order to further reduce the residual waste liquid on the surface of the tissue block, a laminar flow hood 14 is provided above the cleaning pool 1, as shown in the attached figure. Figure 8 The laminar flow hood 14 is provided with a fan inside, and the blowing of the fan in the laminar flow hood 14 and the oscillation of the oscillator 8 can reduce the waste liquid remaining on the tissue block.

[0048] In the embodiment of the present application, a baffle 9 is movably provided at the connection between the receiving tank 4 and the tissue outlet tube 5. Figure 5 、 6 As shown. The bottom outlet of the storage tank 4 can be movably blocked by pulling the baffle 9. Furthermore, the baffle 9 in the embodiment of the present application is connected to a baffle push rod 13, and the baffle 9 can be movably pulled and pulled by pushing the baffle push rod 13. When the tissue block is cleaned or disinfected, the baffle 9 is controlled by the baffle push rod 13 to be pushed to the liquid outlet pipe 6, blocking the bottom outlet of the storage tank 4 and the top inlet of the tissue outlet pipe 5 and the liquid outlet pipe 6. At this time, the cleaning liquid or disinfectant accumulates in the storage tank 4 and the cleaning pool 1. When the cleaning or disinfection is completed, the baffle 9 is controlled to be pulled away by the baffle push rod 13 to open the top inlet of the tissue outlet pipe 5 and the liquid outlet pipe 6. At this time, the waste liquid can be discharged through the liquid outlet pipe 6, and then the cleaned or disinfected tissue block is discharged through the tissue outlet pipe 5.

[0049] An ultraviolet lamp 15 is also provided above the cleaning pool 1. The ultraviolet lamp 15 is located on one side of the laminar flow hood 14. Figure 8 After the tissue block is cleaned or disinfected, the inside of the tissue cleaning and disinfecting device can be disinfected by irradiating with the ultraviolet lamp 15 to keep the inside in a sterile state, thereby facilitating subsequent tissue block sample processing.

[0050] In order to realize the arrangement of the cleaning pool 1, the tissue inlet pipe 2, the liquid inlet pipe 3, the accommodating tank 4 and other components, the tissue cleaning and disinfection device in the embodiment of the present application further includes a housing 19 for supporting the cleaning pool 1, etc. Figure 9 、 10 As shown, a partition 20 is horizontally provided inside the housing 19. The cleaning tank 1 is located on the partition 20. The inlet of the tissue inlet pipe 2, the inlet of the liquid inlet pipe 3, the outlet of the tissue outlet pipe 5, and the outlet of the liquid outlet pipe 6 are all exposed to the housing 19. The laminar flow hood 14 and the ultraviolet lamp 15 are arranged on the top of the housing 19.

[0051] In the embodiments of the present application, all components are preferably made of stainless steel or polytetrafluoroethylene.

[0052] When the tissue cleaning and disinfection device provided in the embodiment of the present application is in use, the baffle 9 is controlled by the baffle push rod 13 to block the bottom outlet of the storage tank 4 and the top inlets of the tissue outlet pipe 5 and the liquid outlet pipe 6. At the same time, the filter push rod 11 controls the two cleaning filters 703 to merge into a flat structure. The tissue inlet cover 16 and the liquid inlet cover 17 are opened, and the tissue block to be cleaned or disinfected is placed on the cleaning filter 703 through the tissue inlet pipe 2. Then, a sterile cleaning liquid or disinfectant is added through the liquid inlet pipe 3. The oscillator 8 is turned on and oscillates the tissue block to ensure thorough cleaning or disinfection. When cleaning or disinfection is complete, the baffle 9 is controlled to be withdrawn by the baffle push rod 13, allowing the top inlet of the liquid outlet pipe 6 to open. At this time, the waste liquid generated by cleaning or disinfection is discharged from the liquid outlet pipe 6. The baffle 9 is then further withdrawn by the baffle push rod 13 until the top inlet of the tissue outlet pipe 5 opens. The two cleaning filters 703 are controlled to be in an open state by the filter push rod 11, and the tissue blocks fall off from between the two cleaning filters 703 into the accommodating groove 4. Under the guidance and positioning action of the positioning filter 12, the tissue blocks smoothly enter the tissue outlet tube 5 and are then discharged into the sterile collection device to achieve sterile collection.

[0053] The tissue cleaning and disinfection device provided in the embodiments of the present application can replace manual cleaning and disinfection methods, eliminating the need to use tweezers to clamp tissue blocks and shake or flip them back and forth in a cleaning or disinfecting solution, thereby improving cleaning or disinfection efficiency. The device in the present application can clean and disinfect different tissue blocks without being placed in a biological safety cabinet or ultra-clean workbench, ensuring that the tissue blocks are sterile, facilitating the isolation of sterile primary cells from the tissue blocks, and ensuring the success rate of primary culture.

[0054] The above description is merely a preferred embodiment of the present application and is not intended to limit the present application. Various modifications and variations are possible for those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. A tissue cleaning and disinfection device, characterized in that: The invention comprises a cleaning pool (1), a tissue inlet pipe (2) and a liquid inlet pipe (3) connected to the top of the cleaning pool (1), and a receiving tank (4) connected to the bottom of the cleaning pool (1), wherein the bottom of the receiving tank (4) is provided with a tissue outlet pipe (5) and a liquid outlet pipe (6), respectively; a cleaning support member (7) with a water leakage hole is provided inside the cleaning pool (1); an oscillator (8) is provided outside the receiving tank (4); a baffle (9) is movably provided at the connection between the receiving tank (4) and the tissue outlet pipe (5), and the baffle (9) movably blocks the bottom outlet of the receiving tank (4).

2. The tissue cleaning and disinfecting device according to claim 1, characterized in that: The cleaning support member (7) is connected to a filter push rod (11) via a connecting rod (10), and the filter push rod (11) is located outside the cleaning tank (1).

3. The tissue cleaning and disinfecting device according to claim 2, characterized in that: The cleaning support member (7) comprises two fixed rods (701) arranged on the inner wall of the cleaning tank (1); each fixed rod (701) is provided with two fixed plates (702), and a cleaning filter (703) is provided between the two fixed plates (702); the fixed plates (702) are movably connected to the filter push rod (11) through the connecting rod (10), and the filter push rod (11) drives the two cleaning filters (703) to rotate relative to each other.

4. The tissue cleaning and disinfecting device according to claim 1, characterized in that: Two positioning filters (12) are vertically provided inside the accommodating tank (4), and the two positioning filters (12) are respectively located at the top of the tissue outlet tube (5), and the distance between the two positioning filters (12) is less than or equal to the diameter or width of the tissue outlet tube (5).

5. The tissue cleaning and disinfecting device according to claim 1, characterized in that: The baffle (9) is connected to a baffle pushing rod (13).

6. The tissue cleaning and disinfecting device according to claim 1, characterized in that: A laminar flow hood (14) and an ultraviolet lamp (15) are provided above the cleaning pool (1), and the ultraviolet lamp (15) is located on one side of the laminar flow hood (14).

7. The tissue cleaning and disinfecting device according to claim 1, characterized in that: A tissue inlet cover (16) is provided on the top of the tissue inlet tube (2), and a liquid inlet cover (17) is provided on the top of the liquid inlet tube (3).

8. The tissue cleaning and disinfecting device according to claim 1, characterized in that: The bottom of the cleaning tank (1) is provided with a plurality of rubber connecting seats (18).

9. The tissue cleaning and disinfecting device according to any one of claims 1 to 8, characterized in that: The tissue cleaning and disinfecting device further comprises a shell (19) and a partition (20) horizontally arranged inside the shell (19); the cleaning pool (1) is located on the partition (20); the inlet of the tissue inlet pipe (2), the inlet of the liquid inlet pipe (3), the outlet of the tissue outlet pipe (5) and the outlet of the liquid outlet pipe (6) are all exposed to the shell (19).

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