Nucleic acid extractor for pig breeding

Abstract

The utility model belongs to the technical field of pig breeding biological analysis and detection, and discloses a pig breeding nucleic acid extraction instrument which comprises an extraction box and a water tank, the water tank is formed in the bottom of the extraction box, a moving seat is arranged in the extraction box, and side grooves are fixedly formed in the two sides, located in the extraction box, of the moving seat; according to the device, the driving motor drives the beating ball to rotate, then the moving seat is driven to reciprocate while vibrating, an extraction reagent in the reagent tube is vibrated and shaken, the extraction efficiency is improved, the trouble of manual shaking is omitted, the extraction efficiency is improved, and the device is suitable for large-scale popularization and application. Meanwhile, by means of disinfectant fluid in the water tank, a worker can put used reagents into the disinfectant fluid, the reagents and an accounting extracting solution are disinfected and sterilized through the disinfectant fluid, virus diffusion is avoided, and the use safety is improved.

Description

Technical Field

[0001] The present application relates to the technical field of pig farming biological analysis and detection, and more specifically, to a nucleic acid extraction instrument for pig farming. Background Art

[0002] Nucleic acid testing is widely used in many fields such as disease control, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, animal husbandry and molecular biology research. In the process of pig breeding, nucleic acid testing is often performed on sick pigs to reduce virus transmission and quickly control the epidemic.

[0003] After searching, it was found that the application number is CN202120916428.6, and the name is a rapid nucleic acid extraction reagent plate and kit. The application raises the problem that the existing column-type nucleic acid extraction and magnetic bead nucleic acid extraction kits use centrifuges, pipettes, vortex instruments, nucleic acid extractors and other laboratory professional tools, and the time period is long. By simplifying and pre-packaging the traditional nucleic acid extraction reagent components (including lysis solution, binding solution, washing solution, and eluent) to form a nucleic acid extraction reagent plate (including lysis binding solution, washing solution, and eluent), the production efficiency is greatly improved and the use is more convenient. The nucleic acid extraction can be completed on site in just a few minutes, which greatly simplifies the operation process and shortens the detection time.

[0004] However, in actual use, due to the shallow depth of the reagent tank, the reagent is very likely to leak when manually shaken. In addition, when extracting nucleic acids, it is difficult to meet the extraction requirements by manual shaking alone, resulting in low extraction efficiency and more troublesome use. At the same time, it is difficult to seal and sterilize after use, which can easily lead to cross infection and affect the safety of use. Further improvements can be made.

[0005] In order to solve the above problems, the present application provides a nucleic acid extraction instrument for pig farming. Utility Model Content

[0006] The present application provides a nucleic acid extraction instrument for pig farming that adopts the following technical solution:

[0007] A nucleic acid extractor for pig farming comprises an extraction box and a water trough, wherein a water trough is provided at the bottom of the extraction box, and a movable seat is provided inside the extraction box, and side grooves are fixedly installed on both sides of the movable seat inside the extraction box, the side grooves are connected to the two ends of the movable seat by springs, and a pipe rack is fixedly installed on the top surface of the movable seat, and a reagent tube is inserted through the top surface of the pipe rack, a driving motor is fixedly installed on the top surface of the extraction box, and a shaft rod is installed at the output end of the driving motor, and the bottom surface of the shaft rod is rotatably connected to the bottom surface of the extraction box through a rotating connecting seat, a hitting ball is fixedly installed on the surface of the shaft rod on one side of the movable seat, a sewage pipe is connected to one end of the water trough, and a liquid pipe is connected to the other end of the water trough, and a waste liquid bucket is connected to the top surface of the liquid pipe, and disinfectant water is contained in the water trough.

[0008] Through the above technical solution, it is convenient to vibrate and shake the extraction reagent inside the reagent tube, thereby improving the extraction efficiency, eliminating the trouble of manual shaking, and improving the convenience of use. At the same time, through the disinfectant water in the sink, the staff can put the reagents to be used into the disinfectant water, and use the disinfectant water to disinfect and sterilize the reagents and nucleic acid extraction solution to avoid the spread of viruses and improve the safety of use.

[0009] Furthermore, a door is hinged on the front facade of the extraction box, and glass is embedded in the surface of the door.

[0010] The above technical solution makes it easy to observe the internal working conditions of the extraction box.

[0011] Furthermore, there are multiple reagent tubes arranged, and tube caps are spirally sleeved on the top surfaces of the reagent tubes.

[0012] Through the above technical solution, the tube cap is convenient for sealing the reagent, and different extraction reagents are respectively contained inside the reagent tube. When in use, the staff can rotate and open the tube cap.

[0013] Furthermore, a battery is fixedly installed at one end of the extraction box, and the output end of the battery is electrically connected to the input end of the drive motor.

[0014] The above technical solution makes it easy to supply power to the driving motor and facilitates the mobile use of the device.

[0015] Furthermore, an equipment box is fixedly installed on the top surface of the extraction box, and a handle is fixedly installed on the top surface of the equipment box.

[0016] The above technical solution makes it easy to store extraction equipment. The nucleic acid extraction equipment is placed inside the equipment box, which is convenient for staff to use, and the handle makes it easy to transfer the device.

[0017] Furthermore, a base is fixedly installed on the bottom surface of the extraction box, and the base is distributed at the corners of the bottom surface of the extraction box.

[0018] The above technical solution facilitates the stable placement of the extraction box.

[0019] Furthermore, a valve is installed on the surface of the sewage pipe, and an upper cover is spirally sleeved on the top surface of the waste liquid bucket.

[0020] Through the above technical solution, the valve is convenient for discharging sewage. After the staff opens the upper cover, they can pour the used reagents into the waste liquid bucket.

[0021] In summary, this application has the following beneficial technical effects:

[0022] By driving the motor to drive the hitting ball to rotate, the movable seat is driven to move back and forth while vibrating, which oscillates and shakes the extraction reagent inside the reagent tube, thereby improving the extraction efficiency, eliminating the trouble of manual shaking, and improving the convenience of use. At the same time, through the disinfectant water in the sink, the staff can put the reagents to be used into the disinfectant water, and use the disinfectant water to disinfect and sterilize the reagents and nucleic acid extract to avoid the spread of viruses and improve the safety of use. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 A schematic diagram of the overall internal structure of this application;

[0024] Figure 2 A schematic diagram of the overall external structure of this application;

[0025] Figure 3 This is a schematic diagram of the installation of the reagent tube of this application;

[0026] Figure 4 This is a schematic diagram of the installation of the hitting ball of this application.

[0027] Description of the numbers in the figure:

[0028] 1. Extraction box; 2. Sink; 3. Disinfectant; 4. Base; 5. Drain pipe; 6. Mobile seat; 7. Side trough; 8. Spring; 9. Tube rack; 10. Reagent tube; 11. Tube cap; 12. Battery; 13. Drive motor; 14. Shaft; 15. Waste liquid bucket; 16. Hitting ball; 17. Rotating connector; 18. Fluid flow pipe; 19. Equipment box; 20. Handle; 21. Box door. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application; it is obvious that the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0030] In the description of this application, it should be noted that the terms "upper," "lower," "inner," "outer," "top / bottom," and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate the description of this application and simplify the description. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0031] In the description of this application, it should be noted that, unless otherwise expressly specified or limited, the terms "installed," "provided with," "mounted / connected," and "connected" should be understood in a broad sense. For example, "connected" can mean a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this application based on the specific circumstances.

[0032] Example:

[0033] This application embodiment discloses a nucleic acid extraction instrument for pig farming. Figure 1 and Figure 4, including an extraction box 1 and a water tank 2, the water tank 2 is opened at the bottom of the extraction box 1, and disinfectant water 3 is contained inside. A movable seat 6 is provided inside the extraction box 1, and the movable seat 6 slides against the bottom surface of the extraction box 1, and side grooves 7 are fixedly installed on both sides of the movable seat 6 inside the extraction box 1. The top surface of the side groove 7 slides against the top surfaces of both ends of the movable seat 6, and a spring 8 is installed between the side groove 7 and the movable seat 6. The two ends of the spring 8 are fixedly connected to the movable seat 6 and the inner wall of the side groove 7 respectively. There are multiple groups of springs 8, which make the movable seat 6 reciprocate through elasticity, and a tube rack 9 is fixedly installed on the top surface of the movable seat 6, and a reagent tube 10 is inserted through the top surface of the tube rack 9. A circular hole for the reagent tube 10 to pass through is opened on the top surface of the tube rack 9, and a groove for holding the bottom of the reagent tube 10 is opened just below the corresponding circular hole on the top surface of the movable seat 6. A driving motor 13 is fixedly installed on the top surface of the extraction box 1, and a shaft 14 is installed on the output end of the driving motor 13, and the bottom surface of the shaft 14 is rotated The rotating connecting seat 17 is rotatably connected to the bottom surface of the extraction box 1, and the rotating connecting seat 17 stabilizes the rotation of the shaft 14. The distance between the shaft 14 and the movable seat 6 is less than the length of the hitting ball 16. The surface of the shaft 14 is located on one side of the movable seat 6 and is fixedly installed with a hitting ball 16. One end of the water tank 2 is connected to the sewage pipe 5, and the other end of the water tank 2 is connected to the liquid pipe 18, and the top surface of the liquid pipe 18 is connected to the waste liquid bucket 15. During nucleic acid extraction, the driving motor 13 drives the hitting ball 16 to rotate, and then drives the movable seat 6 to move back and forth while vibrating, oscillating and shaking the extraction reagent inside the reagent tube 10, thereby improving the extraction efficiency, eliminating the trouble of manual shaking, and improving the convenience of use. At the same time, through the disinfectant water 3 in the water tank 2, the staff can put the reagents to be used into the disinfectant water 3, and disinfect and sterilize the reagents and nucleic acid extraction liquid through the disinfectant water 3 to avoid virus spread and improve the safety of use.

[0034] See also Figure 1 and Figure 2 The front facade of the extraction box 1 is hinged with a box door 21, which is a double-leaf structure and has glass embedded in the surface of the box door 21. The glass makes it easy to observe the working conditions inside the extraction box 1.

[0035] See also Figure 1 and Figure 3 There are multiple reagent tubes 10, and a tube cap 11 is spirally sleeved on the top surface of the reagent tube 10. The tube cap 11 is convenient for sealing the reagent. Different extraction reagents are respectively contained in the inside of the reagent tube 10. When in use, the staff can rotate and open the tube cap 11.

[0036] See also Figure 1 and Figure 2 A battery 12 is fixedly installed at one end of the extraction box 1, and the output end of the battery 12 is electrically connected to the input end of the drive motor. The battery 12 is a common power supply structure, which is convenient for powering the drive motor 13 and facilitating the mobile use of the device.

[0037] See also Figure 1 and Figure 2 An equipment box 19 is fixedly installed on the top surface of the extraction box 1, and a handle 20 is fixedly installed on the top surface of the equipment box 19. Extraction equipment such as test paper and pipette are placed inside the equipment box 19. The lid of the equipment box 19 is connected to the body of the equipment box 19 by a lock, which is convenient for opening. The handle 20 makes it convenient for the staff to carry and transfer the device.

[0038] See also Figure 1 and Figure 2 A base 4 is fixedly installed on the bottom surface of the extraction box 1, and the base 4 is distributed at the corners of the bottom surface of the extraction box 1, so as to facilitate the stable placement of the extraction box 1.

[0039] See also Figure 1 and Figure 2 A valve is installed on the surface of the sewage pipe 5, and a cover is spirally sleeved on the top surface of the waste liquid bucket 15. The valve is closed during nucleic acid extraction. After reaching the centralized waste liquid treatment area, the valve can be opened to discharge the waste liquid. After the staff opens the cover, they can pour the used reagents into the waste liquid bucket 15. The cover facilitates the sealing of the waste liquid bucket 15 to prevent the spread of the virus.

[0040] The implementation principle of this embodiment is: when in use, move the device to a suitable position, open the box door 21 and the tube cap 11, drive the motor 13 to drive the hitting ball 16 to rotate, and then drive the movable seat 6 to move back and forth while vibrating, and oscillate and shake the extraction reagent inside the reagent tube 10, thereby improving the extraction efficiency, eliminating the trouble of manual shaking, and improving the convenience of use. At the same time, through the disinfectant water 3 in the water tank 2, the staff can put the reagents to be used into the disinfectant water 3, and use the disinfectant water 3 to disinfect and sterilize the reagents and nucleic acid extract to avoid virus spread and improve the safety of use.

[0041] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A nucleic acid extraction instrument for pig farming, comprising an extraction box (1) and a water tank (2), characterized in that: The extraction box (1) is provided with a water tank (2) at the bottom, and a movable seat (6) is provided inside the extraction box (1), and side grooves (7) are fixedly installed on both sides of the movable seat (6) inside the extraction box (1), and the side grooves (7) are connected to the two ends of the movable seat (6) through springs (8), and a pipe rack (9) is fixedly installed on the top surface of the movable seat (6), and a reagent tube (10) is inserted through the top surface of the pipe rack (9), and a driving motor (13) is fixedly installed on the top surface of the extraction box (1), and the driving motor (13) ) The output end is provided with a shaft (14), and the bottom surface of the shaft (14) is rotatably connected to the inner bottom surface of the extraction box (1) through a rotating connecting seat (17), and a striking ball (16) is fixedly installed on the surface of the shaft (14) located on one side of the movable seat (6), one end of the water tank (2) is connected to a sewage pipe (5), and the other end of the water tank (2) is connected to a liquid flow pipe (18), and the top surface of the liquid flow pipe (18) is connected to a waste liquid bucket (15), and the inside of the water tank (2) contains disinfectant water (3).

2. A nucleic acid extraction instrument for pig farming according to claim 1, characterized in that: The extraction box (1) is hingedly provided with a box door (21) on the front elevation, and the surface of the box door (21) is embedded with glass.

3. The nucleic acid extraction instrument for pig farming according to claim 1, characterized in that: A plurality of the reagent tubes (10) are arranged, and a tube cap (11) is spirally sleeved on the top surface of the reagent tube (10).

4. The nucleic acid extraction instrument for pig farming according to claim 1, characterized in that: A battery (12) is fixedly mounted on one end of the extraction box (1), and the output end of the battery (12) is electrically connected to the input end of the drive motor (13).

5. The nucleic acid extraction instrument for pig farming according to claim 1, characterized in that: An equipment box (19) is fixedly installed on the top surface of the extraction box (1), and a handle (20) is fixedly installed on the top surface of the equipment box (19).

6. The nucleic acid extraction instrument for pig farming according to claim 1, characterized in that: A base (4) is fixedly mounted on the bottom surface of the extraction box (1), and the base (4) is distributed at the corners of the bottom surface of the extraction box (1).

7. The nucleic acid extraction instrument for pig farming according to claim 1, characterized in that: A valve is installed on the surface of the sewage pipe (5), and an upper cover is spirally sleeved on the top surface of the waste liquid bucket (15).

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