Grabbing and rotating device for biological dissociation

Abstract

The utility model relates to a grabbing and rotating device for biological dissociation, which belongs to the technical field of tissue dissociation equipment and comprises a female head main body, a tube cover, a first bearing and a second bearing, the first bearing and the second bearing are mounted on the outer surface of the female head main body, and a test tube is connected in the tube cover through threads. And a stirring rod is mounted in the test tube. According to the stirring rod grabbing device, the ball head, the groove, the second spring, the third spring and the universal ball are used in cooperation, when a stirring rod is grabbed, the female head body descends through the lifting device, then the ball head enters the groove through the second spring to grab a plunger, grabbing of the stirring rod is achieved, and in the grabbing process, the stirring rod can be grabbed conveniently and rapidly. The tube cover can extrude a universal ball to extrude a third spring, so that downward extrusion force generated by the female head main body on the test tube is buffered, the test tube is prevented from being crushed, the test tube is well protected, and tissue fluid in the test tube is prevented from leaking.

Description

Technical Field

[0001] The utility model belongs to the technical field of tissue dissociation equipment, and in particular relates to a grabbing and rotating device for biological dissociation. Background Art

[0002] In recent years, single-cell transcriptome sequencing technology has been rapidly developed, making it possible to reveal the expression of all genes in the entire genome at the single-cell level, which is very beneficial for studying expression heterogeneity between cells. Currently, single-cell transcriptome sequencing technology has been widely used in different types of tissues and cell lines of various species, including normal and diseased cells. Before single-cell sequencing, the tissue to be sequenced must first be digested and dissociated into a single-cell suspension. The quality of the resulting cell suspension and the richness of the cell types contained in the cell suspension determine the accuracy and reliability of the final detection indicators. Generally, in order to improve the efficiency of dissociation, the temperature is maintained at 37°C during the dissociation process. However, temperature heating is generally performed by heating the test tube with an electric heater, which can easily cause uneven heating of the test tube.

[0003] After searching, a utility model patent with Chinese patent number CN221192165U discloses a device for tissue digestion and dissociation. The above-mentioned prior art uses a rotor clip to grab the connecting rotor. Although the connecting rotor can also be grabbed by this method, the grabbing method is to use squeezing to make the rotor clip grab the connecting rotor. During the squeezing process, the test tube may be crushed, resulting in leakage of tissue fluid in the test tube. Based on this, a grabbing and rotating device for biological dissociation is proposed. Utility Model Content

[0004] The purpose of the present invention is to provide a grabbing and rotating device for biological dissociation with a simple structure and reasonable design in order to solve the above problems.

[0005] The utility model achieves the above-mentioned purpose through the following technical solutions:

[0006] A grasping and rotating device for biological dissociation includes a female head body and a tube cover, as well as a first bearing and a second bearing installed on the outer surface of the female head body. A test tube is connected to the tube cover via a thread, a stirring rod is installed inside the test tube, a plunger is fixedly connected to the top of the stirring rod, a groove is provided on the side wall of the plunger, a connecting rotor is fixedly connected to the top of the plunger, an airway is provided on the female head body, a buckle is installed in the airway, a ball head is installed in the airway, and a universal ball is installed in the airway.

[0007] As a further optimization solution of the present invention, the groove is adapted to the ball head, the connecting turntable is adapted to the buckle, the universal ball is adapted to the pipe cover, the outer surface of the female head body is connected to a gear through a thread, and a push rod is installed in the female head body.

[0008] As a further optimization solution of the present invention, the buckle, ball head and universal ball can slide in the airway, the buckle is fixedly connected in the airway by a first spring, the ball head is fixedly connected in the airway by a second spring, and the universal ball is fixedly connected in the airway by a third spring.

[0009] As a further optimization solution of the present invention, a plurality of stirring grooves are provided on the stirring rod, and the plurality of stirring grooves are arranged in an equidistant array from top to bottom along the side wall of the stirring rod, and the sizes of the plurality of stirring grooves decrease successively from top to bottom.

[0010] As a further optimization scheme of the present invention, a slot is provided at the bottom of the stirring rod, a stirring head is installed at the bottom of the stirring rod, an insertion rod is fixedly connected to the top of the stirring head, the insertion rod is adapted to the slot, the insertion rod and the slot are fixedly connected by a positioning assembly, a paddle is fixedly connected to the side wall of the stirring head, and a plurality of shear columns are fixedly connected below the side wall of the stirring head.

[0011] As a further optimization scheme of the present invention, the positioning assembly includes a slot hole opened on the insertion rod, a telescopic rod is fixedly connected in the slot hole, a connecting spring is sleeved on the telescopic rod, a positioning head is fixedly connected to the free end of the telescopic rod, a positioning groove is opened in the slot, the positioning head is adapted to the positioning groove, and the two ends of the connecting spring are fixedly connected to the positioning head and the slot hole respectively.

[0012] The beneficial effect of the utility model is that: the utility model cooperates with the ball head, the groove, the second spring, the third spring and the universal ball. When grabbing the stirring rod, the female head body is lowered by the lifting device, and then the ball head enters the groove through the second spring to grab the plunger, thereby achieving the grabbing of the stirring rod. During the grabbing process, the tube cover will squeeze the universal ball so that the third spring is squeezed, thereby achieving the buffering of the downward squeezing force generated by the female head body on the test tube, preventing the test tube from being crushed, providing good protection for the test tube, and preventing the tissue fluid in the test tube from leaking. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the utility model;

[0014] Figure 2 This is a schematic diagram of the front middle section structure of the present invention;

[0015] Figure 3This utility model Figure 2 A in the middle is an enlarged structural diagram;

[0016] Figure 4 It is a schematic diagram of the structure of the utility model with the middle part cut away from the side;

[0017] Figure 5 This utility model Figure 4 The enlarged structural diagram at B in the middle;

[0018] Figure 6 It is a three-dimensional partially cutaway structural schematic diagram of the utility model;

[0019] Figure 7 This utility model Figure 6 The enlarged structural diagram at C in the middle;

[0020] Figure 8 It is a schematic diagram of the three-dimensional structure of the stirring rod of the present invention.

[0021] In the figure: 1. female connector body; 2. first bearing; 3. second bearing; 4. gear; 5. ejector pin; 6. tube cover; 7. test tube; 8. stirring rod; 9. stirring tank; 10. plunger; 11. groove; 12. connecting rotor; 13. airway; 14. buckle; 15. ball head; 16. universal ball; 17. slot; 18. insert rod; 19. slot; 20. telescopic rod; 21. connecting spring; 22. positioning head; 23. stirring head; 24. paddle; 25. positioning groove; 26. shear column; 27. first spring; 28. second spring; 29. ​​third spring. DETAILED DESCRIPTION

[0022] The present application is described in further detail below in conjunction with the accompanying drawings. It is necessary to point out that the following specific implementation methods are only used to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application. Technicians in this field can make some non-essential improvements and adjustments to the present application based on the above application content.

[0023] Example

[0024] like Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 and Figure 8As shown, a grabbing and rotating device for biological dissociation includes a female body 1 and a tube cover 6, and a first bearing 2 and a second bearing 3 installed on the outer surface of the female body 1. The female body 1 is installed on the lifting device in the tissue dissociation device through the first bearing 2 and the second bearing 3, and rises and falls synchronously with the lifting device in the tissue dissociation device. A test tube 7 is connected to the tube cover 6 through a thread, and a stirring rod 8 is installed inside the test tube 7. The top of the stirring rod 8 is fixedly connected to a plunger 10, and a groove 11 is provided on the side wall of the plunger 10. The top of the plunger 10 is fixedly connected to a connecting head 12. An airway 13 is provided on the female body 1, a buckle 14 is installed in the airway 13, a ball head 15 is installed in the airway 13, a universal ball 16 is installed in the airway 13, and the groove 11 and the ball head 1 are connected. 5 is adapted, the connecting rotor 12 is adapted to the buckle 14, the connecting rotor 12 is composed of four card plates, the universal ball 16 is adapted to the tube cover 6, the outer surface of the female head body 1 is connected to the gear 4 by a thread, and a push rod 5 is installed in the female head body 1, the push rod 5 is fixedly connected to the tissue dissociation device, the buckle 14, the ball head 15 and the universal ball 16 can slide in the airway 13, the buckle 14 is fixedly connected to the airway 13 by a first spring 27, the ball head 15 is fixedly connected to the airway 13 by a second spring 28, and the universal ball 16 is fixedly connected to the airway 13 by a third spring 29. A plurality of stirring grooves 9 are provided on the stirring rod 8, and the plurality of stirring grooves 9 are arranged in an equidistant array from top to bottom along the side wall of the stirring rod 8, and the sizes of the plurality of stirring grooves 9 decrease sequentially from top to bottom.

[0025] When in use, the test tube 7 is placed in the tissue dissociation device so that the test tube 7 is directly under the female head body 1, and then the lifting device of the tissue dissociation device is started to move the female head body 1 downward. During the downward movement of the female head body 1, the ball head 15 will squeeze the second spring 28 under the squeezing action of the plunger 10, and when the ball head 15 reaches the position of the groove 11, the ball head 15 will enter the groove 11 under the action of the second spring 28, and at this time the buckle 14 will enter between the card plates connected to the rotating head 12, and in this process, the universal ball 16 will be used to press the female head body 12. And the third spring 29 buffers the extrusion force, and then the tissue dissociation device can be started, so that the female head body 1 rotates, and then the stirring rod 8 is driven to rotate through the buckle 14 and the connecting rotor 12 to stir the tissue fluid in the test tube 7, thereby achieving the grasping of the stirring rod 8. During the grasping process, the tube cover 6 will squeeze the universal ball 16 so that the third spring 29 is squeezed, thereby achieving the buffering of the downward extrusion force generated by the female head body 1 on the test tube 7, preventing the test tube 7 from being crushed, and providing good protection for the test tube 7, preventing the tissue fluid in the test tube 7 from leaking.

[0026] After the tissue fluid is stirred, the lifting device moves upward, causing the female head body 1 to drive the stirring rod 8 to move upward. At this time, due to the fixation of the top rod 5, when the connecting head 12 contacts the top rod 5 and the lifting device continues to move upward, it will push the stirring rod 8 to move downward, causing the ball head 15 to disengage from the groove 11 and release the grip on the stirring rod 8.

[0027] like Figure 3 、 Figure 7 and Figure 8 As shown, a slot 17 is provided at the bottom of the stirring rod 8, a stirring head 23 is installed at the bottom of the stirring rod 8, and an insertion rod 18 is fixedly connected to the top of the stirring head 23, which is adapted to the slot 17. The insertion rod 18 and the slot 17 are fixedly connected through a positioning assembly, and a paddle 24 is fixedly connected to the side wall of the stirring head 23. A plurality of shear columns 26 are fixedly connected to the lower side wall of the stirring head 23, and the positioning assembly includes a slot 19 provided on the insertion rod 18, a telescopic rod 20 is fixedly connected in the slot 19, a connecting spring 21 is sleeved on the telescopic rod 20, and a positioning head 22 is fixedly connected to the free end of the telescopic rod 20, and a positioning groove 25 is provided in the slot 17, and the positioning head 22 is adapted to the positioning groove 25, and the two ends of the connecting spring 21 are fixedly connected to the positioning head 22 and the slot 19 respectively.

[0028] When the paddle 24 on the stirring head 23 is deformed or damaged and needs to be repaired or replaced, the tube cover 6 is unscrewed to pull the stirring rod 8 out of the test tube 7. Then, the stirring head 23 can be pulled downward so that the slot 17 squeezes and pushes the positioning head 22 out of the positioning slot 25, and the stirring head 23 can be removed from the stirring rod 8 to repair the paddle 24 on the stirring head 23 or directly replace the stirring head 23. When installing again, the insertion rod 18 is aligned with the slot 17 and pushed inward. During the pushing process, the slot 17 squeezes the positioning head 22 so that the connecting spring 21 and the telescopic rod 20 are compressed. When the positioning head 22 reaches the position of the positioning slot 25, the positioning head 22 will enter the positioning slot 25 under the action of the connecting spring 21 and the telescopic rod 20, fixing the position of the insertion rod 18, and realizing the disassembly and installation of the stirring head 23. The operation is simple and quick, does not require the help of external tools, and is convenient to use.

[0029] The above-described embodiments merely represent several implementation methods of the present invention. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the scope of the present invention, all of which fall within the scope of protection of the present invention.

Claims

1. A grabbing and rotating device for biological dissociation, comprising a female body (1) and a tube cover (6), and a first bearing (2) and a second bearing (3) mounted on the outer surface of the female body (1), characterized in that: The tube cover (6) is connected to a test tube (7) via a thread, a stirring rod (8) is installed inside the test tube (7), a plunger (10) is fixedly connected to the top of the stirring rod (8), a groove (11) is provided on the side wall of the plunger (10), a connecting rotary head (12) is fixedly connected to the top of the plunger (10), an airway (13) is provided on the female head body (1), a buckle (14) is installed in the airway (13), a ball head (15) is installed in the airway (13), and a universal ball (16) is installed in the airway (13).

2. The grabbing and rotating device for biodissociation according to claim 1, characterized in that: The groove (11) is adapted to the ball head (15), the connecting rotating head (12) is adapted to the buckle (14), the universal ball (16) is adapted to the pipe cover (6), the outer surface of the female head body (1) is connected to the gear (4) through a thread, and a push rod (5) is installed in the female head body (1).

3. The grabbing and rotating device for biodissociation according to claim 1, characterized in that: The buckle (14), the ball head (15) and the universal ball (16) can slide in the airway (13); the buckle (14) is fixedly connected to the airway (13) via a first spring (27); the ball head (15) is fixedly connected to the airway (13) via a second spring (28); and the universal ball (16) is fixedly connected to the airway (13) via a third spring (29).

4. The grabbing and rotating device for biodissociation according to claim 3, characterized in that: The stirring rod (8) is provided with a plurality of stirring grooves (9), which are arranged in an equidistant array from top to bottom along the side wall of the stirring rod (8), and the sizes of the plurality of stirring grooves (9) decrease in sequence from top to bottom.

5. The grabbing and rotating device for biodissociation according to claim 4, characterized in that: A slot (17) is provided at the bottom of the stirring rod (8), a stirring head (23) is installed at the bottom of the stirring rod (8), a plug rod (18) is fixedly connected to the top of the stirring head (23), the plug rod (18) is adapted to the slot (17), the plug rod (18) and the slot (17) are fixedly connected via a positioning assembly, a paddle (24) is fixedly connected to the side wall of the stirring head (23), and a plurality of shear columns (26) are fixedly connected below the side wall of the stirring head (23).

6. The grabbing and rotating device for biodissociation according to claim 5, characterized in that: The positioning assembly includes a slot (19) provided on the insertion rod (18), a telescopic rod (20) is fixedly connected in the slot (19), a connecting spring (21) is sleeved on the telescopic rod (20), a positioning head (22) is fixedly connected to the free end of the telescopic rod (20), a positioning groove (25) is provided in the slot (17), the positioning head (22) is adapted to the positioning groove (25), and the two ends of the connecting spring (21) are fixedly connected to the positioning head (22) and the slot (19), respectively.

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