Device for feeding liquid into centrifugal micro-fluidic chip
By designing the liquid inlet device of the liquid storage chamber and the pressure chamber, combined with the check structure and the waterproof and breathable membrane, the problems of liquid addition error and bubble in centrifugal microfluidic chip detection are solved, and accurate liquid addition and simplified operation are achieved.
Patent Information
- Application Number
- CN202422401270.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-30
- Publication Date
- 2025-09-16
- Estimated Expiration
- 2034-09-30
AI Technical Summary
During the centrifugal microfluidic chip detection process, users are not familiar with adding liquids, which can easily lead to large errors in liquid volume and may generate bubbles. The existing technology requires skilled personnel to operate.
A liquid inlet device including a shell and a pair of parts is designed. The shell contains a liquid storage chamber and a pressure chamber. The precise filling of reagents is achieved through a check structure and a pressurizing part. A waterproof and breathable membrane is used to prevent gas from entering the liquid storage chamber. The snap-on structure ensures the quick connection and detachment of the device.
It reduces the error of adding liquid, avoids the generation of bubbles, makes the operation smoother, simplifies the adding process, and is suitable for use by unskilled personnel.
Smart Images
Figure CN223337355U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microfluidics, in particular to a device for liquid feeding into a centrifugal microfluidics chip. Background Art
[0002] When testing centrifugal microfluidic chips, it is necessary to first complete the liquid filling of the microfluidic chip. One liquid reservoir corresponds to one syringe, and each syringe is filled with the required solution according to the reaction requirements. When injecting samples into the microfluidic chip, the inspector inserts the syringe into the liquid inlet hole of the liquid reservoir of the microfluidic chip and presses the syringe to inject the required solution into the corresponding liquid reservoir.
[0003] Therefore, when using centrifugal microfluidic chip testing, relatively skilled personnel are normally required to use a pipette or other disposable dropper to add liquid. If the user is not familiar with adding liquid, it will cause large errors in liquid volume and generate bubbles. Utility Model Content
[0004] The purpose of the present utility model is to provide a device for liquid feeding into a centrifugal microfluidic chip, so as to solve the problems raised in the above background technology.
[0005] To achieve the above objectives, the present invention provides the following technical solutions:
[0006] A device for feeding liquid into a centrifugal microfluidic chip, comprising:
[0007] A housing, the housing comprising a liquid storage chamber and a pressure chamber connected to the liquid storage chamber via a check structure, the liquid storage chamber being used to store reagents;
[0008] The opponent piece is detachably connected to the housing via a snap-fit structure;
[0009] There is a communication hole between the liquid storage chamber and the chamber of the opponent piece;
[0010] The pressurizing part is used to pressurize the pressure chamber so that the reagent stored in the liquid storage chamber enters the chamber of the counterpart through the communicating hole.
[0011] Furthermore, the check structure includes a waterproof and breathable membrane, which is installed in the channel between the liquid storage chamber and the pressure chamber.
[0012] Furthermore, the channel is close to the bottom of the liquid storage chamber and the pressure chamber.
[0013] Furthermore, the buckle structure includes two symmetrical buckles on the shell and symmetrical buckle positions on the counterpart, and the buckle positions have steps that are hooked with the buckles.
[0014] Furthermore, the buckle position and the edge of the step are arc-shaped, the shape of the buckle bottom is adapted to the edge of the step, and the two steps are diagonally distributed in the two buckle positions.
[0015] Furthermore, a groove is provided in the communicating hole at the bottom of the liquid storage chamber, and a sealing ring is provided in the groove.
[0016] Furthermore, the pressurizing part includes a push rod, a rubber plug is provided at the bottom of the push rod, and a pressing handle is provided at the top of the push rod. The rubber plug is installed in the pressure chamber and slides up and down in the pressure chamber.
[0017] Furthermore, the upper portion of the push rod has limiting wings in the circumferential direction.
[0018] Furthermore, the top of the liquid storage chamber is provided with a screw pipe port, and the sealing cover is screwed onto the screw pipe port.
[0019] Furthermore, the side surface of the liquid storage cavity has a sealing plug hole for inserting a sealing plug.
[0020] Compared with the prior art, the beneficial effects of the present invention are:
[0021] The present invention comprises a shell including a liquid storage chamber and a pressure chamber. The liquid storage chamber is used to store reagents. The pressurizing part is used to pressurize the pressure chamber so that the reagents stored in the liquid storage chamber enter the chamber of the counterpart through the connecting hole. The gas compression of the pressure chamber through the check structure can enter the liquid storage chamber through the channel, and the reagents stored in the liquid storage chamber enter the chamber of the counterpart through the through hole, thereby preventing the reagents from leaking out of the liquid storage chamber. Since the pressure chamber is pressurized to add the reagents stored in the liquid storage chamber to the chamber of the counterpart, during the addition process, the gas is at the upper liquid surface of the reagent and the through hole is at the lower liquid surface of the reagent. This will avoid the problem of large liquid errors and bubbles caused by the user when adding liquid. Moreover, pressing the push rod downward in the pressure chamber makes the addition smoother, thereby achieving the purpose of reducing the error of the amount of liquid added by the personnel. Through the buckle structure, the shell and the counterpart can be tightly and quickly combined, and the shell and the counterpart can be quickly detached after the addition of liquid to the counterpart. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 This is a schematic structural diagram of the utility model.
[0023] Figure 2 This is a schematic diagram of the explosion structure of the utility model.
[0024] Figure 3 For this utility model Figure 2 Bottom view of .
[0025] Figure 4 This is a schematic diagram of the assembly of the hand piece and the housing of the present invention.
[0026] Figure 5 This is a schematic diagram of the disassembly of the hand piece and the shell of the utility model.
[0027] Figure 6 For this utility model Figure 1 Cross-sectional view at AA in the middle.
[0028] Figure 7 This is a schematic diagram of the chip used in this utility model.
[0029] In the figure: 1-shell, 2-liquid storage chamber, 3-pressure chamber, 4-pressurizing part, 5-push rod, 6-pressing handle, 7-rubber plug, 8-screw pipe port, 9-sealing cover, 10-sealing plug hole, 11-sealing plug, 12-opposite piece, 13-buckle, 14-buckle position, 15-step, 16-sealing ring, 17-groove, 18-connecting hole, 19-channel, 20-waterproof breathable membrane, 21-limiting wing. DETAILED DESCRIPTION
[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0031] In the description of the present invention, it should be noted that the terms "upper end", "lower end", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end", etc., indicating the orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore should not be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance.
[0032] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "installed," "provided with," "sleeved," "socketed," "connected," etc., should be understood in a broad sense. For example, "connected" may refer to a fixed connection, a detachable connection, or an integral connection; it may refer to a mechanical connection or an electrical connection; it may refer to a direct connection or an indirect connection through an intermediate medium; it may refer to internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on the specific circumstances.
[0033] See also Figures 1 to 7 , the utility model provides a technical solution:
[0034] A device for feeding liquid into a centrifugal microfluidic chip, comprising:
[0035] A housing 1 includes a liquid storage chamber 2 and a pressure chamber 3 connected to the liquid storage chamber 2 via a check structure, wherein the liquid storage chamber 2 is used to store reagents;
[0036] The opponent piece 12 is detachably connected to the housing 1 via a snap-fit structure;
[0037] There is a communication hole 18 between the liquid storage chamber 2 and the chamber of the opponent 12;
[0038] The pressurizing part 4 is used to pressurize the pressure chamber 3 so that the reagent stored in the liquid storage chamber 2 enters the chamber of the counterpart 12 through the communicating hole 18 .
[0039] Specifically, the check structure includes a waterproof breathable membrane 20, which is installed in the channel 19 between the liquid storage chamber 2 and the pressure chamber 3. Figure 6 、 7 As shown, by pressing the push rod 5, the push rod 5 is pressed down, and the gas in the pressure chamber 3 is compressed and enters the liquid storage chamber 2 through the channel 19 and the waterproof breathable membrane 20, thereby allowing the reagent stored in the liquid storage chamber 2 to enter the chamber of the opponent 12 through the through hole 18.
[0040] Since the pressure chamber 3 is used to pressurize the reagent stored in the liquid storage chamber 2 to be added to the chamber of the opponent 12, during the filling process, the gas is at the upper liquid surface of the reagent and the through hole 18 is at the lower liquid surface of the reagent. This will avoid the problem of large liquid errors and bubbles caused by the user when adding liquid, and pressing the push rod 5 downward in the pressure chamber 3 can achieve smoother filling and reduce the error of the liquid adding amount by the user.
[0041] Due to the provision of the waterproof breathable membrane 20, liquid molecules can pass through the air pores of the waterproof breathable membrane 20 during diffusion movement, while the spacing between liquid molecules is smaller than the spacing between the air pores. Under the action of surface tension, the liquid molecules cannot pass through the waterproof breathable membrane, that is, it plays a waterproof role. In this way, the reagent stored in the liquid storage chamber 2 cannot enter the pressure chamber 3 through the channel 19, thereby preventing the reagent from leaking out of the liquid storage chamber 2.
[0042] Specifically, the channel 19 is close to the bottom of the liquid storage chamber 2 and the pressure chamber 3. Figure 6 、 7 As shown, the reagent stored in the liquid storage chamber 2 can be injected as much as possible through the pressurizing part 4.
[0043] Specifically, the buckle structure includes two symmetrical buckles 13 on the housing 1 and symmetrical buckle positions 14 on the counterpart 12. The buckle positions 14 have steps 15 that are hooked with the buckles 13. The edges of the buckle positions 14 and the steps 15 are arc-shaped, and the bottom shape of the buckle 13 is adapted to the edge of the step 15. Figure 4 、 5 As shown, when the hand piece 12 and the housing 1 need to be fixed together, the buckle 13 is aligned with the buckle position 14 of the hand piece 12 and pressed. Then, the housing 1 and the hand piece 12 are rotated 45 degrees relative to each other so that the buckle 13 is hooked with the step 15. In this way, the housing 1 and the hand piece 12 can be tightly and quickly connected. The buckle 13 is located at the bottom of the liquid storage chamber 2.
[0044] The two steps 15 are diagonally distributed in the two buckle positions 14, which can ensure that the housing 1 and the opponent 12 only need to rotate a small angle to achieve a tight connection between the housing 1 and the opponent 12. When disassembly is required, the housing 1 and the opponent 12 can be rotated 45 degrees relative to each other in the opposite direction.
[0045] Specifically, the communicating hole 18 at the bottom of the liquid storage chamber 2 is provided with a groove 17, and a sealing ring 16 is provided in the groove 17. Figure 3 、 6 Shown, after existing housing 1 and adversary 12 are closely combined, guarantee that the connection is leak-proof by compressed sealing ring 16. Certain sealing ring 16 can protrude slightly to groove 17 outside and thereby make compression tighter, and leak-proof effect is better like this.
[0046] Specifically, the pressurizing part 4 includes a push rod 5, a rubber plug 7 is provided at the bottom of the push rod 5, and a pressing handle 6 is provided at the top of the push rod 5. The rubber plug 7 is installed in the pressure chamber 3 and slides up and down in the pressure chamber 3. The upper part of the push rod 5 has a limiting wing 21 on the circumference. Figure 1 、 2 As shown, during use, by pressing the handle 6 downward, the push rod 5 drives the rubber plug 7 downward to pressurize the pressure chamber 3, and the reagent stored in the liquid storage chamber 2 enters the chamber of the opponent 12 through the through hole 18 via the channel 19. The limit wing 21 acts as a limit structure. When the push rod 5 is pushed to a certain position and cannot be pressed down, the liquid filling of the opponent is completed.
[0047] Specifically, the top of the liquid storage chamber 2 is provided with a screw pipe port 8, and a sealing cover 9 is screwed onto the screw pipe port 8. The side of the liquid storage chamber 2 has a sealing plug hole 10 for inserting a sealing plug 11. Figure 6 As shown, the sealing cover 9 is threaded onto the pipe port 8 so as to seal and store the reagent in the liquid storage chamber 2 .
[0048] Specifically, this utility model provides an integrated, detachable liquid-adding device that integrates with a centrifugal microfluidic chip for liquid inflow detection. Designed to reduce errors during liquid addition, the device pre-injects reagents / liquids into the liquid reservoir (i.e., liquid storage chamber 2) before entering the chip. Liquid addition is achieved simply by pushing the push rod 5 and its rubber stopper 7. Once the chamber is filled with liquid, the reservoir can be removed. Operation is simple and easy to understand.
[0049] The process of using this device is as follows:
[0050] Step 1. First, assemble the sealing plug 11 and the sealing ring 16 on the housing 1;
[0051] Step 2. Then pull the push rod 5 up to the highest position, that is, the plug 7 is at the top position in the pressure chamber 3;
[0052] Step 3. Align the buckle 13 of the device with the buckle position 14 of the opponent 12 and press it. Then rotate the housing 1 and the opponent 12 45 degrees relative to each other so that the device and the opponent 12 are tightly combined. The compressed sealing ring 16 ensures that the connection does not leak.
[0053] Step 4. Add the required amount of reagent to the liquid storage chamber 2, cover the sealing cap 9, and complete the addition;
[0054] Step 5. To use, simply push the push rod 5 by pressing the handle 6. The rubber plug 7 presses down the gas in the pressure chamber 3 and squeezes it into the liquid storage chamber 2 through the channel 19. The reagent in the liquid storage chamber 2 enters the chamber of the opponent 12. A limit structure is provided on the push rod 5. After it is pushed to a certain position, the push rod 5 cannot be pressed down further, and the liquid is added to the opponent 12.
[0055] Step 6. Then the housing 1 and the hand piece 12 are rotated 45° counterclockwise to remove the device, and the hand piece 12 can be used for subsequent detection operations.
[0056] The undescribed parts of the present invention are existing or known technologies.
[0057] Although the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A device for feeding liquid into a centrifugal microfluidic chip, characterized in that: include: A housing (1), the housing (1) comprising a liquid storage chamber (2) and a pressure chamber (3) connected to the liquid storage chamber (2) via a check structure, the liquid storage chamber (2) being used to store a reagent; A pair of hand pieces (12), wherein the hand piece (12) is detachably connected to the housing (1) via a snap-fit structure; A communication hole (18) is provided between the liquid storage chamber (2) and the chamber of the counterpart (12); The pressurizing part (4) is used to pressurize the pressure chamber (3) so that the reagent stored in the liquid storage chamber (2) enters the chamber of the counterpart (12) through the connecting hole (18).
2. The device for liquid feeding into a centrifugal microfluidic chip according to claim 1, characterized in that: The anti-return structure comprises a waterproof and breathable membrane (20), and the waterproof and breathable membrane (20) is installed in the channel (19) between the liquid storage chamber (2) and the pressure chamber (3).
3. The device for liquid feeding into a centrifugal microfluidic chip according to claim 2, characterized in that: The channel (19) is close to the bottom of the liquid storage chamber (2) and the pressure chamber (3).
4. The device for liquid feeding into a centrifugal microfluidic chip according to claim 1, wherein: The buckle structure comprises two symmetrical buckles (13) on the housing (1) and a symmetrical buckle position (14) on the counterpart (12); the buckle position (14) has a step (15) that is engaged with the buckle (13).
5. The device for liquid feeding into a centrifugal microfluidic chip according to claim 4, characterized in that: The edges of the buckle position (14) and the step (15) are arc-shaped, the bottom shape of the buckle (13) is adapted to the edge of the step (15), and the two steps (15) are diagonally distributed in the two buckle positions (14).
6. The device for liquid feeding into a centrifugal microfluidic chip according to claim 1, characterized in that: The communicating hole (18) at the bottom of the liquid storage chamber (2) is provided with a groove (17), and a sealing ring (16) is provided in the groove (17).
7. The device for liquid feeding into a centrifugal microfluidic chip according to claim 1, characterized in that: The pressurizing part (4) includes a push rod (5), a rubber plug (7) is provided at the bottom of the push rod (5), and a pressing handle (6) is provided at the top of the push rod (5). The rubber plug (7) is installed in the pressure chamber (3) and slides up and down in the pressure chamber (3).
8. The device for liquid feeding into a centrifugal microfluidic chip according to claim 7, characterized in that: The upper part of the push rod (5) is provided with a limiting wing (21) in the circumferential direction.
9. The device for liquid feeding into a centrifugal microfluidic chip according to claim 1, wherein: The top of the liquid storage chamber (2) is provided with a screw pipe port (8), and a sealing cover (9) is screwed onto the screw pipe port (8).
10. The device for liquid feeding into a centrifugal microfluidic chip according to claim 1, characterized in that: The side surface of the liquid storage chamber (2) is provided with a sealing plug hole (10) for inserting a sealing plug (11).