Stem cell culture device

Abstract

The utility model relates to the technical field of stem cell culture, in particular to a stem cell culture device which comprises a device body, a heat preservation cavity and an installation cavity are vertically distributed in the device body, a plurality of culture frames are vertically arranged in the heat preservation cavity at intervals through a supporting frame, and a low-speed fan and a heating part are installed in the installation cavity. An air outlet pipe is vertically arranged in the middle of the heat preservation cavity, the lower end of the air outlet pipe penetrates into the installation cavity to be communicated with the air outlet of the heating part, the upper end of the air outlet pipe is closed, and a plurality of exhaust outlets communicating the inside and the outside are formed in the side face of the air outlet pipe in the vertical direction. And through the exhaust outlets distributed in the upper edge and the lower edge of the air outlet pipe, heated air can be evenly blown into the heat preservation cavity from the center position in an up-down layered mode, the heated air can be blown into each height area of the heat preservation cavity, and the temperature of the heat preservation cavity can be rapidly and evenly increased.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell culture, in particular to a stem cell culture device. Background Art

[0002] Stem cell research plays a crucial role in the biomedical field, with applications spanning numerous key areas, including tissue repair, disease treatment, and drug development. Stem cell culture, the cornerstone of stem cell research and application, requires a high-quality, stable, and rigorously controlled culture environment to ensure the growth, proliferation, and differentiation of stem cells.

[0003] Currently, common stem cell culture devices on the market suffer from significant deficiencies and shortcomings in several key areas. Temperature control, a crucial factor in stem cell culture, is a crucial factor in many existing devices. However, many of these devices lack optimal temperature control accuracy and stability.

[0004] Precise temperature control is crucial for the survival of stem cells and the maintenance of their normal physiological functions. The optimal temperature for stem cell culture is typically around 37°C, close to normal human body temperature, which helps maintain the normal physiological functions and metabolic activity of stem cells. Even small temperature fluctuations can have profound effects on stem cell metabolic processes, gene expression, and protein synthesis. For example, excessively low temperatures can slow cell metabolism and reduce enzyme activity, thereby affecting cell growth and division rates. Excessively high temperatures, on the other hand, can cause protein denaturation, cell membrane damage, and even lead to cell apoptosis.

[0005] Room temperature is generally below 37°C. Therefore, in order to create a temperature suitable for stem cell culture, the culture device generally heats the internal temperature. However, due to design defects in the temperature control system of some existing culture devices, there may be uneven temperature inside the culture device, resulting in local temperatures that are too high or too low, thus affecting the stem cell culture in certain locations. Utility Model Content

[0006] The purpose of the present invention is to provide a stem cell culture device to solve the problem in the prior art that uneven temperature may exist inside the culture device, resulting in local excessively high or low temperatures, thereby affecting the stem cell culture in certain locations.

[0007] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0008] A stem cell culture device comprises a device body, wherein a heat preservation chamber and an installation chamber are distributed in the upper and lower parts of the device body, a plurality of culture racks are placed in the heat preservation chamber at intervals of upper and lower parts by a support frame, a low-speed fan and a heating unit are installed in the installation chamber, the air outlet of the low-speed fan is connected to the air inlet of the heating unit, an air outlet pipe is vertically arranged in the middle of the heat preservation chamber, the lower end of the air outlet pipe passes through the installation chamber and is connected to the air outlet of the heating unit, the upper end is closed, a plurality of exhaust ports communicating with the inside and outside are distributed in the upper and lower parts on the side of the air outlet pipe; a plurality of return air ducts are vertically arranged on the cavity wall of the heat preservation chamber, the lower ends of the plurality of return air ducts all pass through the installation chamber and are connected to the air inlet of the low-speed fan, and the side walls of the plurality of return air ducts are distributed in the upper and lower parts on the plurality of return air ports communicating with the inside and outside.

[0009] A further technical solution is that an air outlet chamber, a heating chamber and an air inlet chamber are distributed along the upper and lower edges of the heating part, the lower end of the air outlet pipe is connected to the air outlet chamber, a heating coil is provided in the heating chamber, and the air outlet of the low-speed fan is connected to the air inlet chamber; the air outlet chamber is connected to the heating chamber through a first air duct, and the air inlet chamber is connected to the heating chamber through a second air duct, and the first air duct and the second air duct are respectively provided at opposite ends of the heating chamber.

[0010] A further technical solution is that an air outlet chamber, a heating chamber and an air inlet chamber are distributed along the upper and lower edges of the heating part, the lower end of the air outlet pipe is connected to the air outlet chamber, a heating coil is provided in the heating chamber, and the air outlet of the low-speed fan is connected to the air inlet chamber; the air outlet chamber is connected to the heating chamber through a first air duct, and the air inlet chamber is connected to the heating chamber through a second air duct, and the first air duct and the second air duct are respectively provided at opposite ends of the heating chamber.

[0011] A further technical solution is that a filter tube is provided in the air inlet cavity, an air outlet of the low-speed fan is connected to one end of the filter cavity in the air inlet cavity, and filter cotton is provided in the filter tube.

[0012] A further technical solution is that a side of the heating portion is provided with an inspection port connected to the air inlet chamber, and an inspection door for closing the inspection port.

[0013] A further technical solution is that a first temperature sensor is installed on the inner wall of the lower end of the air outlet pipe, a second temperature sensor is installed on the lower side of the culture rack, and a third temperature sensor is provided on the heating coil.

[0014] A further technical solution is that a monitoring camera is installed on the wall of the heat preservation chamber.

[0015] A further technical solution is that a guide rail is vertically installed on the wall of the insulation chamber, a lead screw motor is vertically installed at the lower end of the guide rail, the lead screw of the lead screw motor is vertically parallel to the guide rail, the thread on the lead screw is matched with a drive block, the drive block is connected to the guide rail by a slide bar on the guide rail and slides up and down, and the monitoring camera is installed on the drive block.

[0016] A further technical solution is that an operating port communicating with the heat preservation chamber and a heat-insulating transparent sliding door for closing the operating port are provided on the front side of the device body.

[0017] Compared with the prior art, the present invention has at least one of the following beneficial effects: 1. The air in the insulation chamber close to the chamber wall is extracted through a low-speed fan and a return air duct, and after being heated and heated by the heating part, it is blown back into the insulation chamber through the air outlet duct, so that the temperature in the insulation chamber can be increased when the temperature in the insulation chamber drops; 2. The exhaust ports distributed up and down on the upper edge of the air outlet duct can make the heated air evenly blown into the insulation chamber from the center position in layers up and down, so that each height area of ​​the insulation chamber can be blown in with heated air, so that the insulation chamber can be heated quickly and evenly, and with the help of the return air ports on the return air duct corresponding to the exhaust ports, a good air flow can be formed, further improving the heating uniformity in the insulation chamber; 3. By setting a low-speed fan, a low-speed airflow can be generated, which can avoid the influence of high-speed airflow on the culture rack in the insulation chamber. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is an overall schematic diagram of a stem cell culture device of the present invention.

[0019] Figure 2 This is a schematic diagram of the interior of a stem cell culture device of the present invention.

[0020] Figure 3 This is a schematic cross-sectional view of the heating portion of a stem cell culture device of the present invention.

[0021] Figure 4 This is a schematic diagram of a lead screw motor and guide rails of a stem cell culture device of the present invention.

[0022] Figure 5 for Figure 4 A partial enlarged schematic diagram of the area marked A.

[0023] Icons: 1-Device body, 2-Insulation chamber, 3-Installation chamber, 4-Support frame, 5-Cultivation rack, 6-Low-speed fan, 7-Heating part, 8-Air outlet duct, 9-Exhaust port, 10-Return air duct, 11-Air outlet chamber, 12-Heating chamber, 13-Air inlet chamber, 14-Heating coil, 15-First air duct, 16-Second air duct, 17-Controller, 18-Filter tube, 19-Filter cotton, 20-Inspection door, 21-First temperature sensor, 22-Second temperature sensor, 23-Monitoring camera, 24-Guide rail, 25-Screw motor, 26-Screw, 27-Drive block, 28-Slide bar, 29-Operation port, 30-Insulated transparent sliding door, 31-Drive hole, 32-Third temperature sensor. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0025] Figures 1 to 5 Shown is an embodiment of the present utility model.

[0026] Example 1:

[0027] A stem cell culture device includes a device body 1, in which an insulation chamber 2 and an installation chamber 3 are distributed in the upper and lower parts of the device body 1, a plurality of culture racks 5 are placed in the insulation chamber 2 at intervals of upper and lower parts by a support frame 4, a low-speed fan 6 and a heating part 7 are installed in the installation chamber 3, the air outlet of the low-speed fan 6 is connected to the air inlet of the heating part 7, an air outlet duct 8 is vertically arranged in the middle of the insulation chamber 2, the lower end of the air outlet duct 8 passes through the installation chamber 3 and is connected to the air outlet of the heating part 7, and the upper end is closed, and a plurality of exhaust ports 9 connecting the inside and the outside are distributed along the upper and lower sides of the air outlet duct 8; a plurality of return air ducts 10 are vertically arranged on the cavity wall of the insulation chamber 2, the lower ends of the plurality of return air ducts 10 all pass through the installation chamber 3 and are connected to the air inlet of the low-speed fan 6, and the side walls of the plurality of return air ducts 10 are distributed along the upper and lower sides with a plurality of return air ports connecting the inside and the outside. The air in the insulation chamber 2 near the cavity wall is extracted by the low-speed fan 6 and the return air duct 10, and after being heated and heated by the heating unit 7, it is blown back into the insulation chamber 2 through the outlet pipe 8. In this way, the temperature in the insulation chamber 2 can be increased when the temperature in the insulation chamber 2 drops. The exhaust ports 9 distributed up and down along the outlet pipe 8 can make the heated air evenly blown into the insulation chamber 2 from the center position in layers up and down, so that each height area of ​​the insulation chamber 2 can have heated air blown in, so that the insulation chamber 2 can be heated quickly and evenly. In addition, with the help of the return air ports corresponding to the exhaust ports 9 on the return air duct 10, a good air flow can be formed, further improving the heating uniformity in the insulation chamber 2. By providing the low-speed fan 6, a low-speed airflow can be generated, which prevents the high-speed airflow from affecting the culture rack 5 in the insulation chamber 2.

[0028] An air outlet chamber 11, a heating chamber 12, and an air inlet chamber 13 are arranged in the upper and lower parts of the heating section 7. The lower end of the air outlet pipe 8 is connected to the air outlet chamber 11. A heating coil 14 is arranged in the heating chamber 12. The air outlet of the low-speed fan 6 is connected to the air inlet chamber 13. The air outlet chamber 11 is connected to the heating chamber 12 through a first air duct 15, and the air inlet chamber 13 is connected to the heating chamber 12 through a second air duct 16. The first air duct 15 and the second air duct 16 are respectively arranged at opposite ends of the heating chamber 12. With this arrangement, when the low-speed fan 6 draws air from the heat-insulating chamber 2 into the heating section 7, the air will first enter the air inlet chamber 13, then enter the heating chamber 12 through the second air duct 16, and then enter the air outlet chamber 11 through the second air duct 16 after being heated by the heating coil 14 in the heating chamber 12, and then enter the heat-insulating chamber 2 through the air outlet pipe 8. This arrangement allows the air to be fully heated and heated by the heating coil 14 after entering the heating portion 7, and to be raised to the set insulation temperature.

[0029] A controller 17 for controlling the temperature of the heating coil 14 is installed outside the heating portion 7. By setting the controller 17, the temperature of the heating coil 14 can be controlled and adjusted according to different needs and situations. The heating coil 14 can be an electric heating coil 14.

[0030] A filter tube 18 is provided in the air inlet chamber 13. The air outlet of the low-speed fan 6 is connected to one end of the filter chamber in the air inlet chamber 13. Filter cotton 19 is provided in the filter tube 18. By providing the filter tube 18 and the filter cotton 19, the air entering the heating part 7 can be filtered, reducing some dust accumulated during the repeated use of the air and improving the air quality.

[0031] The side of the heating unit 7 is provided with an access port connected to the air inlet chamber 13 and an access door 20 for sealing the access port. The access port and access door 20 facilitate maintenance of the air inlet chamber 13, such as replacement of the filter tube 18 or filter pad 19. The access port also communicates with the heating chamber 12 and the air outlet chamber 11.

[0032] A first temperature sensor 21 is mounted on the inner wall of the lower end of the air outlet duct 8, a second temperature sensor 22 is mounted on the lower side of the culture rack 5, and a third temperature sensor 32 is mounted on the heating coil 14. The first temperature sensor 21 allows the temperature of the air entering the air outlet duct 8 to be monitored, the second temperature sensor 22 allows the temperature of different areas within the heat preservation chamber 2 to be monitored, and the third temperature sensor 32 allows the temperature of the heating coil 14 to be monitored.

[0033] A surveillance camera 23 is mounted on the wall of the thermal chamber 2. This allows operators to monitor the culture status through the images captured by the surveillance camera 23. This reduces the need to open the thermal transparent sliding door 30 for inspection, thereby preventing temperature fluctuations in the thermal chamber 2 caused by door opening, which could affect the stem cell culture results.

[0034] A guide rail 24 is vertically installed on the wall of the insulation chamber 2, and a screw motor 25 is vertically installed at the lower end of the guide rail 24. The screw 26 of the screw motor 25 is vertically arranged parallel to the guide rail 24. A driving block 27 is connected to the screw 26 by a threaded matching connection. The driving block 27 is connected to the guide rail 24 by sliding a slide bar 28 on the guide rail 24 and sliding up and down. The monitoring camera 23 is installed on the driving block 27. When the screw motor 25 is started, it drives the screw 26 to rotate. The driving block 27 is sleeved on the screw 26 using the driving hole 31. The driving hole 31 can use a thread that matches the screw 26, or use a ball that matches the thread of the screw 26 in the driving hole 31. The multiple balls roll in the gaps between the threads of the screw 26, thereby facilitating the rotation of the screw 26 in the driving hole 31. Due to the limitation of the slide bar 28, when the screw 26 rotates, the driving block 27 cannot rotate with it. Therefore, the balls rotate with the screw 26 and screw into the thread gap of the screw 26 in the direction of the thread, thereby driving the driving block 27 to move up and down. In this way, the monitoring camera 23 can be driven up and down to capture different images of the culture rack 5.

[0035] The front side of the device body 1 is provided with an operation port 29 communicating with the heat preservation chamber 2, and a heat preservation transparent sliding door 30 for closing the operation port 29. The operation port 29 is provided to facilitate operation by the operator, and the heat preservation transparent sliding door 30 can play a role in heat preservation and heat insulation, such as double-layer vacuum laminated glass, and can also facilitate the operator to open the heat preservation transparent sliding door 30 to check the internal situation.

[0036] Although the present invention has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and implementations may be devised by those skilled in the art that fall within the scope and spirit of the principles disclosed herein. More specifically, within the scope of the present disclosure, the drawings, and the claims, various variations and modifications may be made to the components and / or layout of the subject combination arrangement. In addition to variations and modifications to the components and / or layout, other uses will also be apparent to those skilled in the art.

Claims

1. A stem cell culture device, comprising a device body (1), characterized in that: The device body (1) is provided with an insulation chamber (2) and an installation chamber (3) distributed in the upper and lower parts. A plurality of culture racks (5) are placed in the insulation chamber (2) at intervals of up and down by a support frame (4). A low-speed fan (6) and a heating part (7) are installed in the installation chamber (3). The air outlet of the low-speed fan (6) is connected to the air inlet of the heating part (7). An air outlet pipe (8) is vertically provided in the middle of the insulation chamber (2). The lower end of the air outlet pipe (8) passes through the installation chamber (3) and The air outlets of the heating part (7) are connected and the upper end is closed. The side of the air outlet pipe (8) is provided with a plurality of air outlets (9) connected to the inside and outside distributed along the upper and lower sides. A plurality of return air pipes (10) are vertically provided on the cavity wall of the heat preservation cavity (2). The lower ends of the plurality of return air pipes (10) are all passed through the installation cavity (3) and are connected to the air inlet of the low-speed fan (6). The side walls of the plurality of return air pipes (10) are provided with a plurality of return air outlets connected to the inside and outside distributed along the upper and lower sides.

2. The stem cell culture device according to claim 1, characterized in that: An air outlet chamber (11), a heating chamber (12) and an air inlet chamber (13) are arranged in an upper and lower distribution in the heating portion (7); the lower end of the air outlet pipe (8) is connected to the air outlet chamber (11); a heating coil (14) is arranged in the heating chamber (12); the air outlet of the low-speed fan (6) is connected to the air inlet chamber (13); the air outlet chamber (11) is connected to the heating chamber (12) through a first air duct (15); the air inlet chamber (13) is connected to the heating chamber (12) through a second air duct (16); the first air duct (15) and the second air duct (16) are respectively arranged at opposite ends of the heating chamber (12).

3. The stem cell culture device according to claim 2, wherein: A controller (17) for controlling the temperature of the heating coil (14) is installed outside the heating portion (7).

4. The stem cell culture device according to claim 2, wherein: A filter tube (18) is provided in the air inlet cavity (13), an air outlet of the low-speed fan (6) is connected to one end of the filter tube in the air inlet cavity (13), and filter cotton (19) is provided in the filter tube (18).

5. The stem cell culture device according to claim 4, characterized in that: The side of the heating portion (7) is provided with an inspection port connected to the air inlet chamber (13), and an inspection door (20) for closing the inspection port.

6. The stem cell culture device according to claim 2, characterized in that: A first temperature sensor (21) is installed on the inner wall of the lower end of the air outlet pipe (8), a second temperature sensor (22) is installed on the lower side of the culture rack (5), and a third temperature sensor (32) is provided on the heating coil (14).

7. The stem cell culture device according to claim 1, characterized in that: A monitoring camera (23) is installed on the cavity wall of the heat preservation cavity (2).

8. The stem cell culture device according to claim 7, characterized in that: A guide rail (24) is vertically mounted on the wall of the heat preservation chamber (2), a screw motor (25) is vertically mounted on the lower end of the guide rail (24), a screw rod (26) of the screw motor (25) is vertically arranged parallel to the guide rail (24), a driving block (27) is connected to the screw rod (26) by a matching thread, and the driving block (27) is connected to the guide rail (24) by sliding bars (28) on the guide rail (24) in an upward and downward sliding manner, and the monitoring camera (23) is mounted on the driving block (27).

9. The stem cell culture device according to claim 1, characterized in that: The front side of the device body (1) is provided with an operating port (29) communicating with the heat preservation chamber (2), and a heat preservation transparent sliding door (30) for closing the operating port (29).

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