Aeration type thermophilic microbial agent domestication / propagation system

Abstract

The utility model relates to the technical field of fermentation, and discloses an aeration type thermophilic microbial agent domestication / propagation system which comprises a heat preservation box (1), an air compressor (2), a flow meter (3), a fermentation tank (4) and an aeration stone (5), wherein the heat preservation box (1) is connected with the air compressor (2), and the air compressor (2) is connected with the flow meter (3); the heat preservation box (1) comprises a box body (11), a gas channel (12), a cavity (13), a support (14) and a temperature control device. The support (14) is arranged in the cavity (13) and comprises a support frame and support strips, the support strips are installed on the support frame in parallel, and the fermentation tank (4) is arranged between every two adjacent support strips; the fermentation tank (4) is also connected with the flow meter (3); the system further comprises a sterile filter (6), and the sterile filter (6) is connected with the heat preservation box (1). The aeration type thermophilic microbial agent domestication / expanding culture system provided by the utility model can be used for performing domestication / expanding culture research on microorganisms under high-temperature sterile conditions.

Description

Technical Field

[0001] The utility model relates to the field of microbial treatment of solid organic waste, and in particular to an aerated thermophilic bacteria acclimation / expansion culture system. Background Art

[0002] With the acceleration of China's urbanization and the continuous improvement of people's living standards, the production of solid organic waste, such as kitchen waste, has also increased annually. Aerobic fermentation technology is an effective way to realize the resource utilization of solid organic waste. It can transform perishable waste, such as kitchen waste and leftover vegetables, through the action of microorganisms, ultimately forming compost products such as humus. This compost can be used as a fertilizer, with the advantages of improving soil structure, maintaining soil physical and chemical properties, and regulating soil microbial balance.

[0003] In the aerobic fermentation technology of solid organic waste, microorganisms are the core of aerobic composting technology and the key driving factor of compost decomposition. Adding exogenous microbial agents during the composting process can promote the diversity of microbial communities and stimulate the proliferation of beneficial bacteria.

[0004] Exogenous microbial agents are generally obtained through the domestication and expansion of microbial agents. At present, the commonly used equipment for domestication of microbial agents is a shaker and a water bath constant temperature shaking device. Among them, the long-term working temperature of the shaker is mostly below 55°C, and it cannot be used to carry out the domestication of high-temperature resistant microbial agents (for example, thermophilic microorganisms that can withstand temperatures above 60°C). Although the water bath constant temperature shaking device can domesticate thermophilic bacteria, its capacity is limited and it is difficult to maintain a sterile environment. Moreover, shaker bottles are often used in shakers and water bath constant temperature shaking devices. The amount of liquid in the shaker bottle generally does not exceed 2 / 3 of the bottle volume. In order to increase dissolved oxygen, a larger liquid surface is required. When microorganisms are cultured at a higher temperature, the nutrient solution evaporates faster, and the long domestication process requires multiple additions of nutrient solution, which increases the risk of contamination.

[0005] Therefore, there is an urgent need to provide an aerated thermophilic agent acclimation / expansion system with strong temperature control capability, low contamination risk and the ability to conduct microbial acclimation / expansion research in a sterile environment. Summary of the Invention

[0006] The present invention aims to address, at least to a certain extent, one of the technical problems in the related art. To this end, the present invention provides an aerated thermophilic bacteria acclimation / expansion system. The aerated thermophilic bacteria acclimation / expansion system provided in the present invention can conduct microbial acclimation / expansion research in a high-temperature, sterile environment, enabling batch experiments and repeated sterilization. It has the advantages of simple equipment, ease of operation, and a wide range of applications.

[0007] In order to achieve the above-mentioned object, the utility model provides an aerated thermophilic bacteria acclimation / expansion system, wherein the system comprises an insulated box 1, an air compressor 2, a flow meter 3, a fermentation tank 4 and an aeration stone 5; wherein the insulated box 1 is connected to the air compressor 2, and the air compressor 2 is connected to the flow meter 3;

[0008] The insulated box 1 includes a box body 11, a gas channel 12, a cavity 13, a bracket 14 and a temperature control device; the bracket 14 is arranged in the cavity 13, including a bracket frame and a bracket bar, the bracket bar is installed in parallel on the bracket frame, and the fermentation tank 4 is arranged between two adjacent bracket bars; the fermentation tank 4 is also connected to the flow meter 3; the system also includes a sterile filter 6, and the sterile filter 6 is connected to the insulated box 1.

[0009] Through the above technical solution, the beneficial technical effects achieved by the utility model are as follows:

[0010] 1) The aerated thermophilic bacteria acclimation / expansion system provided in the present invention uses a cylindrical fermentation tank with a large height-to-diameter ratio (≥2.5), which increases the volume of the nutrient solution. The tank cover design reduces the evaporation of the nutrient solution at high temperatures. In addition, the outlet pipe and the exhaust port are vertically long. This vertical pipe allows some of the liquid carried away by the air path to flow back, ensuring that most bacterial colonies that require long-term acclimation do not need to be refilled with nutrient solution during the acclimation period, effectively avoiding contamination.

[0011] 2) The aerated thermophilic bacteria acclimation / expansion system provided by the present invention can achieve refined control of the fermentation temperature by providing an insulated box; by providing a sterile filter, a sterile environment can be provided for the fermentation tank; and by providing an aeration stone in the fermentation tank, the aeration effect in the fermentation tank can be significantly improved;

[0012] 3) The aerated thermophilic bacteria acclimation / expansion culture system provided in the present invention can accommodate multiple fermentation tanks in an insulated box, enabling simultaneous exploratory experiments with different parameters, significantly accelerating the experimental process;

[0013] 4) The aerated thermophilic bacteria acclimation / expansion culture system provided in the present invention is easy to disassemble and assemble, has a simple manufacturing process, can be quickly mass-produced, has low cost, has a wide range of uses, and is suitable for industrial promotion.

[0014] Additional aspects and advantages of the present invention will be given in part in the following description and will become apparent from the following description or learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic structural diagram of the aerated thermophilic bacteria acclimation / expansion culture system provided in the present invention;

[0016] Figure 2 This is a schematic structural diagram of the fermentation tank provided in the present invention being installed on a bracket;

[0017] Figure 3 This is a structural diagram of a bracket with a fixed steel ring provided in the utility model;

[0018] Figure 4 It is a structural diagram of the clamp provided in the utility model;

[0019] Figure 5 This is a top view of the fermentation tank cover provided in the present invention;

[0020] Figure 6 It is a structural schematic diagram of the fermentation tank provided in the utility model;

[0021] Figure 7 It is a structural schematic diagram of the fixing gasket provided in the utility model.

[0022] Description of Reference Numerals

[0023] 1. Insulation box 2. Air compressor 3. Flow meter

[0024] 4. Fermentation tank 5. Aeration stone 6. Sterile filter

[0025] 7. Liquid Collection Tube

[0026] 11, box 12, gas channel 13, cavity

[0027] 14, bracket 15, air outlet pipe

[0028] 41, tank body 42, cover plate 43, connecting parts

[0029] 44, fixed gasket

[0030] 141, fixed steel ring 142, silicone gasket

[0031] 421, sampling port 422, exhaust hole 431, clamp

[0032] 411, drain port 412, drain valve 413, air inlet DETAILED DESCRIPTION

[0033] The embodiments of the present invention are described in detail below. The described embodiments are exemplary and intended to be used to explain the present invention, but should not be understood as limiting the present invention.

[0034] The endpoints of the ranges and any values ​​disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoints of each range, the endpoints of each range and individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered to be specifically disclosed herein.

[0035] The utility model provides an aerated thermophilic bacteria acclimation / expansion system, wherein the system comprises an insulated box 1, an air compressor 2, a flow meter 3, a fermentation tank 4 and an aeration stone 5; wherein the insulated box 1 is connected to the air compressor 2, and the air compressor 2 is connected to the flow meter 3;

[0036] The insulated box 1 includes a box body 11, a gas channel 12, a cavity 13, a bracket 14, and a temperature control device; the bracket 14 is arranged in the cavity 13, including a bracket frame and a bracket bar, the bracket bar is installed in parallel on the bracket frame, and the fermenter 4 is arranged between two adjacent bracket bars; the fermenter 4 is also connected to the flow meter 3;

[0037] The system further comprises a sterile filter 6, which is connected to the insulated box 1. Figure 1-2 shown.

[0038] In this utility model, the insulated box can provide a stable temperature for the fermenter, meeting the different ambient temperature requirements for different research purposes such as microbial acclimation and expansion, and avoiding the situation where the fermentation temperature does not meet the standard. The sterile filter can provide a sterile environment for the fermenter, and the aeration stone placed in the fermenter can significantly improve the aeration effect in the fermenter, providing sufficient oxygen for the microorganisms. By configuring the insulated box, sterile filter, aeration stone, and fermenter, the aerated thermophilic microbial acclimation and expansion system of this utility model can be used to study the acclimation and expansion of microorganisms in a high-temperature sterile environment.

[0039] In a preferred embodiment of the present invention, the gas channel 12 runs through the box 11 and the cavity 13 , one end of which is connected to the sterile filter 6 , and the other end of which is connected to the air compressor 2 for heating the sterile gas in the gas channel 12 .

[0040] Among them, in the present invention, the external gas enters the gas channel through the air compressor, can be heated under the action of the heat preservation box, and then is extracted by the air compressor and transported to the fermentation tank after being quantitatively measured by the flow meter.

[0041] In a preferred embodiment of the present invention, the brackets 14 are multiple. In the present invention, the cavity of the insulated box may be provided with a single layer of brackets or multiple layers of brackets. The number of brackets provided can be adjusted based on actual needs and is not specifically limited in the present invention. For example, the number of brackets provided may be 1-3 layers.

[0042] In a preferred embodiment of the present invention, a fixed steel ring 141 is fixedly provided on the bracket 14, a silicone gasket 142 is provided on the inner wall of the fixed steel ring, and the fermentation tank (4) is provided in the high temperature resistant silicone gasket 142. Figure 3 shown.

[0043] In the present invention, to prevent the fermenter from sliding relative to the bracket, a fixed steel ring can be fixed to the bracket to position the fermenter. A silicone gasket can protect the fermenter and cushion any collisions between the fermenter and the fixed steel ring. Preferably, the fixed steel ring 141 is welded between two adjacent bracket bars of the bracket 14, and the silicone gasket 142 is preferably a high-temperature resistant silicone gasket.

[0044] In a preferred embodiment of the present invention, the temperature control device is disposed in the box 11 for performing programmed temperature increase.

[0045] In a preferred embodiment of the present invention, the temperature control device includes a heater and a controller. This invention does not impose any particular limitations on the temperature control device; any temperature control device capable of implementing a programmable temperature ramp function may be used in this invention. The heater may be an electric heating tape, which can uniformly heat and insulate the fermenter, ensuring a constant temperature while providing precise programmable temperature control. Preferably, the temperature control device in this invention has a temperature control range of 25-85°C.

[0046] In a preferred embodiment of the present invention, the insulated box 1 further comprises an air outlet pipe 15, wherein the air outlet pipe 15 is connected to the fermentation tank 4. In the present invention, the gas generated in the fermentation tank can be discharged through the air outlet pipe.

[0047] In a preferred embodiment of the present invention, the air compressor 2 and the flow meter 3 are both conventional devices in the field, and the present invention does not specifically limit them. Preferably, the air compressor 2 is preferably an electric compressor, and the flow meter 3 is preferably a rotor flow meter.

[0048] In a preferred embodiment of the present invention, the fermentation tank includes a tank body 41 and a cover plate 42 arranged on the tank body 41, and the connection method between the cover plate 42 and the tank body 41 can be selected from one of threaded connection, buckle connection, hinge connection or clamp connection.

[0049] In a preferred embodiment of the present invention, the cover plate 42 and the tank body 41 are connected by a clamp through a connecting component 43. The connecting component 43 includes a clamp 431 and a gasket. Figure 4 In the present invention, the material of the clamp is preferably stainless steel, and the gasket is preferably heat-resistant silicone. The clamp and the gasket can seal the cover plate to prevent leakage.

[0050] In a preferred embodiment of the present invention, the cover plate 42 is provided with a sampling port 421 and an exhaust hole 422. Figure 5 shown.

[0051] In the present invention, the vent 422 on the cover plate 42 is connected to the air outlet pipe 15 provided in the cavity 13 of the insulated box 1, allowing the gas generated in the fermenter 4 to be promptly discharged outside the insulated box 1, facilitating the collection of gas samples. Through the sampling port, the fermenter can be added with the inoculum to be acclimated, the nutrient solution, and the target substrate, and the fermentation status within the fermenter can be observed. Preferably, the sampling port is provided with a sealing member.

[0052] In a preferred embodiment of the present invention, the tank body 41 is a hollow cavity, preferably a cylindrical hollow tank. Furthermore, preferably, the height-to-diameter ratio of the cylindrical hollow tank is ≥ 2.5, preferably 2.5-3.5. The tank body is preferably made of a material that can be sterilized under high temperature and high pressure, such as stainless steel.

[0053] In a preferred embodiment of the present invention, the bottom of the tank body 41 is provided with a drain port 411, a drain valve 412 and an air inlet 413, and the air inlet 413 is connected to the flow meter 3. Figure 6 shown.

[0054] In a preferred embodiment of the present invention, the outer side of the tank body 41 further includes at least two fixing pads 44 for mounting the fermentation tank 4 on the bracket 14 in the insulation box 1. Figure 6 shown.

[0055] Among them, in the present invention, the fixed gasket is fixedly connected to the tank body, and is preferably symmetrically arranged on the outside of the tank body. The fixed gasket can be directly clamped on two adjacent bracket bars in the bracket 14, or directly clamped on the fixed steel ring, so that the fermentation tank is placed in the insulation box.

[0056] In a preferred embodiment of the present invention, the fermentation tanks 4 include a plurality, preferably 10-60, of which the exhaust holes 422 of each fermentation tank 4 are connected to the exhaust pipe 15 .

[0057] Among them, in the present invention, multiple fermentation tanks are provided, and multiple experiments can be carried out at the same time, which helps to improve the experimental efficiency, save the experimental cost, and speed up the experimental process.

[0058] In a preferred embodiment of the present invention, the aeration stone 5 is a round cake-shaped aeration stone. In the present invention, the diameter of the round cake-shaped aeration stone matches the inner diameter of the tank body, which can further improve the aeration effect.

[0059] In a preferred embodiment of the present invention, the sterile filter 6 is preferably a two-stage sterile filter. In the present invention, the sterile filter can sterilize the gas entering the fermenter, ensuring that the gas is sterile and that the bacterial agent is acclimated in a sterile environment. The present invention does not impose any particular restrictions on the sterile filter; any commonly used sterile filter in the art can be used in the present invention.

[0060] In a preferred embodiment of the present invention, the aerated thermophilic bacteria acclimation / expansion system may further include a liquid collection pipe 7 , wherein the liquid collection pipe 7 is connected to the drain valve 412 .

[0061] Among them, in the present invention, when the aerated thermophilic bacteria acclimation / expansion system is used for bacteria expansion, a liquid collection pipe can be provided to facilitate the collection of liquid in each fermentation tank.

[0062] In a preferred embodiment of the present invention, the pipe used for gas circulation in the aerated thermophilic bacteria acclimation / expansion system is made of a material that can be sterilized in a high-pressure sterilizer, such as heat-resistant plastic / silicone material.

[0063] In a preferred embodiment of the present invention, in order to facilitate cleaning and sterilization, the connecting pipes between the various devices in the aerated thermophilic bacteria acclimation / expansion system are preferably detachable pipes.

[0064] The embodiments of the present invention are described in detail below. It should be noted that the embodiments described below are exemplary and are only used to explain the present invention, and should not be understood as limiting the present invention.

[0065] The aerated thermophilic bacteria acclimation / expansion system in the embodiment includes an insulated box, an electric compressor, a rotor flowmeter, a fermentation tank, a round cake-shaped aeration stone and a two-stage sterile filter;

[0066] The insulated box is equipped with two layers of brackets. Each layer consists of a bracket frame and bracket bars. A fixed steel ring is welded between adjacent bracket bars, and a high-temperature resistant silicone gasket is installed inside the fixed steel ring. Each layer of brackets has 20 fixed steel rings, each of which houses a fermentation tank. The fermentation tank is a cylindrical hollow tank with a height-to-diameter ratio of 2.5. It consists of a cover, a separate upper and lower tank body, and two connecting parts, including stainless steel clamps and heat-resistant silicone gaskets.

[0067] Example 1

[0068] (1) First, connect the two-stage sterile filter, incubator, electric air compressor, rotor flowmeter, and time relay, and then clean and sterilize the incubator, fermentation tank, and aeration stone;

[0069] (2) First, use the connecting parts to connect the upper tank body and the lower tank body with a clamp, place the aeration stone in the fermentation tank, and then use the connecting parts to connect the upper tank body and the cover plate with a clamp. Then, add the acclimated bacterial agent, sterilized nutrient solution and target substrate to the fermentation tank in sequence through the sampling port. After the addition is completed, the sampling port is sealed to prevent contact with the external environment;

[0070] (3) Insert 40 fermentation tanks into the fixed steel ring on the bracket in sequence, install them on the fixed steel ring with the help of fixed gaskets, then connect the exhaust hole of the fermentation tank to the outlet pipe of the insulation box cavity, and connect the air inlet of the fermentation tank to the flow meter;

[0071] (4) Setting the temperature rise program of the insulated box, and when the temperature reaches the set value, turning on the electric air compressor to aerate the nutrient solution in the fermentation tank;

[0072] (5) During the test, gas samples were collected at any time through the gas outlet pipe for analysis; after the test, the programmable temperature rise heater of the incubator was turned off, the electric air compressor was turned off, the drain valve at the bottom of the fermentation tank was opened, and the bacterial solution was removed for analysis;

[0073] (6) After use, remove the detachable parts, disassemble each part, clean it, and restore it to its original state for next use.

[0074] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine different embodiments or examples described in this specification and features of different embodiments or examples without contradiction.

[0075] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are illustrative and cannot be understood as limitations on the present invention. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. An aerated thermophilic bacteria acclimation / expansion system, characterized in that: The system comprises an insulated box (1), an air compressor (2), a flow meter (3), a fermentation tank (4) and an aeration stone (5); Wherein, the heat preservation box (1) is connected to the air compressor (2), and the air compressor (2) is connected to the flow meter (3); The heat preservation box (1) comprises a box body (11), a gas channel (12), a cavity (13), a bracket (14) and a temperature control device; the bracket (14) is arranged in the cavity (13), and comprises a bracket frame and bracket bars, the bracket bars are installed in parallel on the bracket frame, and the fermentation tank (4) is arranged between two adjacent bracket bars; the fermentation tank (4) is also connected to the flow meter (3); The system further comprises a sterile filter (6), and the sterile filter (6) is connected to the thermal insulation box (1).

2. The aerated thermophilic bacteria acclimation / expansion cultivation system according to claim 1, characterized in that: The gas channel (12) passes through the box (11) and the cavity (13), one end of which is connected to the sterile filter (6) and the other end of which is connected to the air compressor (2), and is used to heat the gas in the gas channel (12); And / or, the temperature control device is arranged in the box (11), and the temperature control device includes a heater and a controller, and is used for performing programmed temperature increase.

3. The aerated thermophilic bacteria acclimation / expansion system according to claim 1, characterized in that: The heat preservation box (1) further comprises an air outlet pipeline (15); wherein the air outlet pipeline (15) is connected to the fermentation tank (4) and is used to discharge the gas generated in the fermentation tank (4).

4. The aerated thermophilic bacteria acclimation / expansion cultivation system according to claim 3, characterized in that: The fermentation tank (4) comprises a tank body (41) and a cover plate (42) arranged on the tank body (41); wherein the connection mode between the cover plate (42) and the tank body (41) is selected from one of a threaded connection, a press connection, a hinge connection or a clamp connection.

5. The aerated thermophilic bacteria acclimation / expansion cultivation system according to claim 4, characterized in that: The cover plate (42) and the tank body (41) are connected by a clamp via a connecting component (43); wherein the connecting component (43) comprises a clamp (431) and a gasket.

6. The aerated thermophilic bacteria acclimation / expansion cultivation system according to claim 5, characterized in that: The cover plate (42) is provided with a sampling port (421) and an exhaust hole (422); wherein the exhaust hole (422) is connected to the exhaust pipe (15).

7. The aerated thermophilic bacteria acclimation / expansion cultivation system according to claim 4, characterized in that: The bottom of the tank body (41) is provided with a liquid discharge port (411), a liquid discharge valve (412) and an air inlet (413), and the air inlet (413) is connected to the flow meter (3).

8. The aerated thermophilic bacteria acclimation / expansion cultivation system according to claim 4, characterized in that: The outer side of the tank body (41) further includes at least two fixing pads (44); wherein the fixing pads (44) are used to mount the fermentation tank (4) on the bracket (14) in the heat preservation box (1).

9. The aerated thermophilic bacteria acclimation / expansion system according to claim 6, characterized in that: The fermentation tank (4) includes a plurality of fermentation tanks, and the exhaust hole (422) of each fermentation tank (4) is connected to the exhaust pipe (15).

10. The aerated thermophilic bacteria acclimation / expansion system according to claim 1, characterized in that: The aeration stone (5) is a round cake-shaped aeration stone, and the sterile filter (6) is a two-stage sterile filter.

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