Efficient culture medium

Abstract

The utility model relates to the technical field of culture media, and provides an efficient culture medium which comprises a working table, a cover plate is fixedly connected to the top of the working table, a fixing mechanism is arranged in the cover plate, a measuring cup is placed at the position, located in front of the cover plate, of the top of the working table, and a supporting plate is fixedly connected to the top of the cover plate; a stirring rod is arranged, a shake flask is arranged, a Kanning 0.22 micron filter is used for filtering, then a mixture is poured into the shake flask 16, 2g / L CM01 is added, a label is pasted, specific components are written, the CM01 maintains the highest living cell density and the best motility rate, and compared with an initial serum-free culture medium, the suspension growth performance of PK15 cells is greatly improved. Compared with a culture medium which always uses serum, the culture medium added with the CM01 can remove dependence of cells on serum, and compared with other serum-free culture mediums, the culture medium added with the CM01 can realize better multiplication speed, higher peak density and better cell state.

Description

Technical Field

[0001] The utility model relates to the technical field of culture medium, in particular to a high-efficiency culture medium. Background Art

[0002] Culture medium refers to a nutrient matrix composed of a combination of different nutrients that supports the growth and reproduction of microorganisms, plants, or animals (or tissues). It generally contains several major substances, including carbohydrates, nitrogen-containing substances, inorganic salts (including trace elements), vitamins, and water. Culture medium not only provides basic nutrients and promotes cell proliferation, but also serves as the living environment for cell growth and reproduction. There are many types of culture media. Based on the source of the raw materials used, they can be divided into natural, synthetic, and semi-synthetic media; based on their physical state, they can be divided into solid, liquid, and semi-solid media; based on their culture function, they can be divided into basal, selective, processing, and identification media; and based on their scope of use, they can be divided into bacterial, actinomycete, yeast, and fungal media.

[0003] However, during the current culture experiments, it was found that serum-free culture medium has certain problems with low suspension growth, poor doubling rate, and low peak density. Culture medium with serum will cause cells to be overly dependent on serum, which will lead to poor cell state.

[0004] In view of this, the utility model proposes a high-efficiency culture medium. Utility Model Content

[0005] The utility model provides a high-efficiency culture medium, which solves the problems of culture medium in related technologies.

[0006] The technical solution of the present utility model is as follows: a high-efficiency culture medium, comprising a workbench, the top of the workbench is fixedly connected to a cover plate, the inside of the cover plate is provided with a fixing mechanism, the top of the workbench is located in front of the cover plate and a measuring cup is placed, the top of the cover plate is fixedly connected to a support plate, the inside of the support plate is fixedly connected to an electric telescopic rod, the output end of the electric telescopic rod is fixedly connected to a lifting plate, the top of the lifting plate is provided with a servo motor, the output end of the servo motor is fixedly connected to a disc, the bottom of the disc is fixedly connected to a fixing column, the bottom of the fixing column is embedded with a stirring rod, the surface of the stirring rod is penetrated by a fixing bolt, the top of the workbench is located on the side of the cover plate and a storage box body is placed, the surface of the storage box body is rotatably connected to a box door, the inside of the storage box body is fixedly connected with a layer plate, the top of the workbench is located in front of the storage box body and a shaking bottle is placed, and the surface of the workbench is provided with a storage cabinet.

[0007] Preferably, the fixing mechanism includes a threaded rod, a slider, a transmission plate, a transmission rod and a fixed plate, the surface of the threaded rod is threadedly connected to the slider, the top of the slider is rotatably connected to the transmission plate, the top of the transmission plate is rotatably connected to the transmission rod, and the surface of the transmission rod is fixedly connected to the fixed plate.

[0008] Preferably, a groove matching a measuring cup is provided on the top of the workbench, and an optimized culture medium is provided inside the measuring cup, wherein the culture medium components inside the measuring cup include: aluminum chloride, biotin, calcium pantothenate, choline chloride, cobalt chloride, copper sulfate, glucose, ethanolamine, ferric citrate, folic acid, glutathione, 4-hydroxyethylpiperazineethanesulfonic acid, inositol, arginine, asparagine, aspartic acid, cysteine, glutamic acid, histidine hydrochloride, hydroxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, magnesium chloride, manganese chloride, nicotinamide, Pluronic F68, potassium chloride, potassium iodide, putrescine, thiamine, vitamin B2, sodium chloride, disodium hydrogen phosphate, sodium pyruvate, spermine, vitamin B1, vitamin B12, and zinc chloride.

[0009] Preferably, the fixing post is arranged to deviate from the center of the disc, and the diameter of the disc is consistent with the inner diameter of the measuring cup.

[0010] Preferably, a circular groove matching the stirring rod is provided at the bottom of the fixing column, and fixing grooves matching the fixing bolts are provided on the surface of the fixing column and the surface of the stirring rod.

[0011] Preferably, a transparent observation port is provided on the surface of the box door, a label is provided on the surface of the shaking bottle, and 2g / LCM01 is added to the inside of the shaking bottle.

[0012] Preferably, the transmission plates, transmission rods and fixed plates are respectively provided in two groups, the transmission plates are arranged in an inclined manner, and the fixed plates are arranged in a semicircular ring shape.

[0013] Preferably, two groups of transversely arranged sliding grooves are opened on the top of the workbench, and the two groups of transmission rods respectively pass through the tops of the two groups of transmission plates and are embedded in the two groups of transverse grooves on the top of the workbench.

[0014] The working principle and beneficial effects of the utility model are as follows:

[0015] In the present invention, a shake flask is provided, filtered through a Corning 0.22 μm filter, then poured into the shake flask, 2 g / L of CM01 is added, and a label is affixed with the specific ingredients. CM01 maintains the highest viable cell density and the best viability. Compared to the original serum-free medium, the suspension growth performance of PK15 cells has been greatly improved. Compared to the previous serum-based medium, the medium supplemented with CM01 can eliminate the cells' dependence on serum. Compared to other serum-free media, the medium supplemented with CM01 can achieve a faster doubling rate, higher peak density, and better cell status.

[0016] In the utility model, a fixing mechanism is provided, so that a worker can embed a measuring cup into a groove on the top of a workbench, and then fix the measuring cup through the fixing mechanism to prevent the measuring cup from tipping over due to force when the culture medium inside the measuring cup is stirred and mixed into a liquid, thereby ensuring the stability of the measuring cup. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The present invention will be further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0018] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0019] Figure 2 This is a schematic diagram of the fixed plate structure of the present utility model;

[0020] Figure 3 This is a schematic diagram of the lifting plate structure of the utility model;

[0021] Figure 4 This is a schematic diagram of the fixed column structure of the utility model;

[0022] Figure 5 This is a schematic diagram of the main structure of the storage box of the present utility model.

[0023] In the figure: 1. Workbench; 2. Cover plate; 3. Fixing mechanism; 31. Threaded rod; 32. Slider; 33. Transmission plate; 34. Transmission rod; 35. Fixing plate; 4. Measuring cup; 5. Support plate; 6. Electric telescopic rod; 7. Lifting plate; 8. Servo motor; 9. Disc; 10. Fixing column; 11. Stirring rod; 12. Fixing bolt; 13. Storage box body; 14. Box door; 15. Shelf; 16. Shaking bottle; 17. Storage cabinet. DETAILED DESCRIPTION

[0024] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention. Example 1

[0025] A preferred embodiment of the high-efficiency culture medium provided by the present invention is as follows Figures 1 to 5 As shown: A high-efficiency culture medium includes a workbench 1, a cover plate 2 is fixedly connected to the top of the workbench 1, a fixing mechanism 3 is arranged inside the cover plate 2, a measuring cup 4 is placed on the top of the workbench 1 in front of the cover plate 2, a support plate 5 is fixedly connected to the top of the cover plate 2, an electric telescopic rod 6 is fixedly connected to the inside of the support plate 5, the output end of the electric telescopic rod 6 is fixedly connected to the lifting plate 7, a servo motor 8 is arranged on the top of the lifting plate 7, a disc 9 is fixedly connected to the output end of the servo motor 8, a fixing column 10 is fixedly connected to the bottom of the disc 9, a stirring rod 11 is embedded in the bottom of the fixing column 10, a fixing bolt 12 passes through the surface of the stirring rod 11, a storage box body 13 is placed on the top of the workbench 1 at the side of the cover plate 2, a box door 14 is rotatably connected to the surface of the storage box body 13, a layer plate 15 is fixedly connected to the inside of the storage box body 13, a shaking bottle 16 is placed on the top of the workbench 1 in front of the storage box body 13, and a storage cabinet 17 is arranged on the surface of the workbench 1.

[0026] In this embodiment, a groove matching the measuring cup 4 is provided on the top of the workbench 1. An optimized culture medium is provided inside the measuring cup 4. The culture medium components inside the measuring cup 4 include: aluminum chloride, biotin, calcium pantothenate, choline chloride, cobalt chloride, copper sulfate, glucose, ethanolamine, ferric citrate, folic acid, glutathione, 4-hydroxyethylpiperazineethanesulfonic acid, inositol, arginine, asparagine, aspartic acid, cysteine, glutamic acid, histidine hydrochloride, hydroxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, magnesium chloride, manganese chloride, nicotinamide, Pluronic F68, potassium chloride, potassium iodide, putrescine, thiamine, vitamin B2, sodium chloride, disodium hydrogen phosphate, sodium pyruvate, spermine, vitamin B1, vitamin B12, and zinc chloride. The measuring cup 4 is embedded in the groove on the top of the workbench 1, and the culture medium is mixed into a liquid. After the mixing is completed.

[0027] In this embodiment, the fixed column 10 is set away from the center of the disk 9, and the diameter of the disk 9 is consistent with the inner diameter of the measuring cup 4. The servo motor 8 is started. Since the disk 9 is fixedly connected to the output end of the servo motor 8, when the servo motor 8 is started, its output end will drive the disk 9 to rotate together. At this time, the fixed column 10 fixedly connected to the disk 9 will then drive the stirring rod 11 to make a circular motion around the center of the disk 9 through the fixing bolt 12, thereby achieving the purpose of stirring.

[0028] In this embodiment, a circular groove matching the stirring rod 11 is provided at the bottom of the fixing column 10, and a fixing groove matching the fixing bolt 12 is provided on the surface of the fixing column 10 and the surface of the stirring rod 11. The stirring rod 11 is embedded in the circular groove at the bottom of the fixing column 10 so that the fixing groove on the surface of the stirring rod 11 corresponds to the fixing groove on the surface of the fixing column 10. Then the fixing bolt 12 is passed through the fixing grooves on the surfaces of the fixing column 10 and the stirring rod 11, and the fixing bolt 12 is tightened to connect the stirring rod 11 to the fixing column 10.

[0029] In this embodiment, a transparent observation port is provided on the surface of the box door 14, a label is provided on the surface of the shake flask 16, and 2 g / LCM01 is added to the shake flask 16. Because the surface of the box door 14 is provided with a transparent observation port, it is convenient for staff to observe the storage situation inside the storage box body 13. Because the surface of the shake flask 16 is provided with a label, the staff can write the specific components of the culture medium on the label and then stick it on the surface of the shake flask 16 to facilitate staff viewing and classification. After the culture medium in the measuring cup 4 is mixed, it is filtered with a Corning 0.22 um filter, then poured into the shake flask 16, and 2 g / LCM01 is added. Then, the specific components are written on the label. CM01 maintains the highest viable cell density and the best viability. Compared with the initial serum-free culture medium, the suspension growth performance of PK15 cells has been greatly improved. Compared with the culture medium that has been using serum before, the culture medium with added CM01 can eliminate the cell's dependence on serum. Compared with other serum-free culture media, the culture medium with added CM01 can achieve a better doubling rate, a higher peak density, and a better cell state. Example 2

[0030] On the basis of Example 1, a preferred embodiment of a high-efficiency culture medium provided by the present invention is as follows Figures 1 to 5 As shown: the fixing mechanism 3 includes a threaded rod 31, a slider 32, a transmission plate 33, a transmission rod 34 and a fixed plate 35. The surface of the threaded rod 31 is threadedly connected to the slider 32, the top of the slider 32 is rotatably connected to the transmission plate 33, the top of the transmission plate 33 is rotatably connected to the transmission rod 34, and the surface of the transmission rod 34 is fixedly connected to the fixed plate 35.

[0031] In this embodiment, two groups of transmission plates 33, transmission rods 34 and fixed plates 35 are respectively provided. The transmission plates 33 are arranged at an angle, and the fixed plates 35 are arranged in a semicircular ring shape. Because the slider 32 is engaged with the threaded rod 31, when the threaded rod 31 rotates, it will drive the slider 32 to slide backward along the inner wall of the slide groove at the top of the workbench 1. At this time, the two groups of transmission plates 33 rotationally connected to the slider 32 will swing left and right relative to each other.

[0032] In this embodiment, two groups of transversely arranged sliding grooves are provided on the top of the workbench 1, and the two groups of transmission rods 34 respectively penetrate the tops of the two groups of transmission plates 33 and are embedded in the two groups of transverse grooves on the top of the workbench 1. Because the two groups of transmission rods 34 are respectively rotatably connected to the two groups of transmission plates 33, and the two groups of transmission rods 34 respectively penetrate the tops of the two groups of transmission plates 33 and are embedded in the two groups of transverse grooves on the top of the workbench 1, when the transmission plates 33 swing relative to each other, the two groups of transmission rods 34 will be driven to slide relative to each other in the two groups of transverse grooves on the top of the workbench 1 and approach each other. At this time, the two groups of fixed plates 35 fixedly connected to the two groups of transmission rods 34 will move relative to each other and approach each other. When the two groups of fixed plates 35 move until they are completely in contact with the measuring cup 4, the measuring cup 4 can be fixed.

[0033] The working principle and usage process of the present invention are as follows: First, the stirring rod 11 is inserted into the circular groove at the bottom of the fixed column 10 so that the fixing groove on the surface of the stirring rod 11 corresponds to the fixing groove on the surface of the fixed column 10, and then the fixing bolt 12 is passed through the fixing grooves on the surfaces of the fixed column 10 and the stirring rod 11, and the fixing bolt 12 is tightened to connect the stirring rod 11 to the fixed column 10. Subsequently, the culture medium is poured into the interior of the measuring cup 4, and then the measuring cup 4 is inserted into the groove on the top of the workbench 1. At this time, the threaded rod 31 is manually controlled to rotate counterclockwise. Because the slider 32 is engaged with the threaded rod 31, when the threaded rod 31 rotates, it will drive the slider 32 to slide backward along the inner wall of the slide groove on the top of the workbench 1. At this time, the two sets of transmission plates 33 rotatably connected to the slider 32 will swing left and right relative to each other, because the two sets of transmission rods 34 are respectively rotatably connected to the two sets of transmission plates 33, and the two sets of transmission rods 34 respectively pass through the top of the two sets of transmission plates 33 and are embedded in the two sets of horizontal grooves on the top of the workbench 1, so when the transmission When the movable plate 33 swings relative to each other, it will drive the two sets of transmission rods 34 to slide relative to each other in the two sets of horizontal grooves on the top of the workbench 1 and approach each other. At this time, the two sets of fixed plates 35 fixedly connected to the two sets of transmission rods 34 will move relative to each other and approach each other. When the two sets of fixed plates 35 move to completely fit with the measuring cup 4, the measuring cup 4 can be fixed. Subsequently, the electric telescopic rod 6 is started to drive the lifting plate 7 to descend. At this time, the lifting plate 7 will drive the servo motor 8, the disc 9 and the fixed column 10 to descend together, thereby driving the stirring rod 11 to descend into the inside of the measuring cup 4. Then, the servo motor 8 is started. Because the disc 9 is fixedly connected to the output end of the servo motor 8, when the servo motor 8 is started, its output end will drive the disc 9 to rotate together. At this time, the fixed column 10 fixedly connected to the disc 9 will drive the stirring rod 11 to make a circular motion around the center of the disc 9 through the fixing bolt 12, thereby achieving the purpose of stirring, thereby replacing manual stirring to ensure the uniformity of the culture medium liquid mixing and improving work efficiency.

[0034] After mixing, the mixture was filtered through a Corning 0.22 μm filter and poured into shake flask 16. 2 g / LC CM01 was added and the specific ingredients were then labeled. CM01 maintained the highest viable cell density and the best viability. Compared with the initial serum-free medium, the suspension growth performance of PK15 cells was greatly improved. Compared with the previous serum-based medium, the medium supplemented with CM01 can eliminate the cells' dependence on serum. Compared with other serum-free media, the medium supplemented with CM01 can achieve a faster doubling rate, higher peak density, and better cell status.

[0035] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A high-efficiency culture medium, comprising a workbench (1), characterized in that: The top of the workbench (1) is fixedly connected to a cover plate (2), a fixing mechanism (3) is provided inside the cover plate (2), a measuring cup (4) is placed on the top of the workbench (1) in front of the cover plate (2), the top of the cover plate (2) is fixedly connected to a support plate (5), the inside of the support plate (5) is fixedly connected to an electric telescopic rod (6), the output end of the electric telescopic rod (6) is fixedly connected to a lifting plate (7), a servo motor (8) is provided on the top of the lifting plate (7), the output end of the servo motor (8) is fixedly connected to a disc (9), the bottom of the disc (9) is fixedly connected to the lifting plate (7), and the lifting plate (7) is fixedly connected to the lifting plate (7). A fixing column (10) is fixedly connected, a stirring rod (11) is embedded in the bottom of the fixing column (10), and a fixing bolt (12) is passed through the surface of the stirring rod (11). The top of the workbench (1) is located on the side of the cover plate (2) and a storage box body (13) is placed. The surface of the storage box body (13) is rotatably connected to a box door (14), and the interior of the storage box body (13) is fixedly connected to a layer plate (15). The top of the workbench (1) is located in front of the storage box body (13) and a shaking bottle (16) is placed. The surface of the workbench (1) is provided with a storage cabinet (17).

2. A high-efficiency culture medium according to claim 1, characterized in that The fixing mechanism (3) comprises a threaded rod (31), a slider (32), a transmission plate (33), a transmission rod (34) and a fixed plate (35); the surface of the threaded rod (31) is threadedly connected to the slider (32); the top of the slider (32) is rotatably connected to the transmission plate (33); the top of the transmission plate (33) is rotatably connected to the transmission rod (34); and the surface of the transmission rod (34) is fixedly connected to the fixed plate (35).

3. A high-efficiency culture medium according to claim 1, characterized in that The fixing column (10) is arranged to deviate from the center of the disc (9), and the diameter of the disc (9) is consistent with the inner diameter of the measuring cup (4).

4. A high-efficiency culture medium according to claim 1, characterized in that The bottom of the fixing column (10) is provided with a circular groove matching the stirring rod (11), and the surfaces of the fixing column (10) and the stirring rod (11) are both provided with fixing grooves matching the fixing bolts (12).

5. A high-efficiency culture medium according to claim 1, characterized in that The surface of the box door (14) is provided with a transparent observation port, the surface of the shaking bottle (16) is provided with a label, and 2g / LCM01 is added into the shaking bottle (16).

6. A high-efficiency culture medium according to claim 2, characterized in that The transmission plate (33), the transmission rod (34) and the fixed plate (35) are respectively provided in two groups. The transmission plate (33) is arranged in an inclined manner, and the fixed plate (35) is arranged in a semicircular ring shape.

7. A high-efficiency culture medium according to claim 2, characterized in that Two groups of transversely arranged sliding grooves are provided on the top of the workbench (1), and the two groups of transmission rods (34) respectively penetrate the tops of the two groups of transmission plates (33) and are embedded in the two groups of transverse grooves on the top of the workbench (1).

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