Glass slide assembly for fat cell experiment

Abstract

The utility model belongs to the technical field of biological experiment instruments and equipment, and particularly relates to a glass slide assembly for fat cell experiments, which comprises a glass slide table, an experiment area is arranged on a first surface of the glass slide table, a containing groove is formed in the experiment area, a third cover glass corresponding to the containing groove is further arranged on the glass slide table, and the third cover glass is provided with a second cover glass corresponding to the containing groove. The third cover glass and the containing groove jointly define a containing space used for containing the adipose cells, a liquid injection channel and a liquid suction channel are formed in the two opposite sides of the containing groove respectively, and through the structure, the rapid building type anti-loss monolayer glass slide assembly special for the adipose cells is comprehensively provided.

Description

Technical Field

[0001] The utility model belongs to the technical field of biological experimental instruments and equipment, and particularly relates to a glass slide assembly for fat cell experiments. Background Art

[0002] Cell culture refers to a technique in which cells collected from in vivo tissues are placed in a sterile environment, under certain nutritional conditions, at a suitable temperature and pH, to simulate the in vivo growth environment, so that they can grow, reproduce, and maintain their structure and function. Cell culture can directly observe the morphological structure, life activities and changes of living cells. It plays an important role in the research and application of cytology, genetics, virology, and immunology. Cell culture usually requires the use of cell culture devices, such as cell culture incubators, cell culture plates, etc. Among them, cell culture plates are the most common biological instruments for culturing cells. It should be noted that the number, quality and uniformity of the monolayer of cells in the cell culture plate will be directly related to the subsequent experimental results. Therefore, the damage to the monolayer of cells in the cell culture plate during the experiment will directly affect the authenticity and reliability of the subsequent experimental results.

[0003] Typically, adherent cells grown in cell culture plates are less firmly attached than those grown in cell culture flasks. Adding or changing sample solutions during an experiment can easily cause the cell monolayer to detach, leading to significant errors in experimental results. To facilitate real-time observation of cells, bacteria are sometimes cultured directly on glass slides. This culture method requires a glass slide / stage and coverslip to secure the cells. However, fragile cells such as adipocytes are particularly susceptible to rupture due to the pressure of the slide / stage and coverslip, leading to various solutions.

[0004] For example, the Chinese invention patent publication number CN109652311A discloses a fixing device for inoculating cells on an ordinary glass slide, comprising a glass slide base provided with a glass slide insertion area and a cell limiting device separate from the glass slide base; this solution saves the step of making cell slides by providing a fixing device for inoculating cells directly onto the glass slide, and avoids the problems of the original ordinary slides that are easy to break, difficult to clamp, and easy to lose.

[0005] While the above solution can prevent cell damage to a certain extent, it still has at least the following problems: complex structure, low slide utilization, and difficulty in changing the liquid. It is also not suitable for fat cells, which are usually located in the upper layer of the droplet and are easily broken once the cover glass is placed. In addition, the above solution cannot achieve a single layer of fat cells, which will affect the observation and imaging of fat cells. Therefore, a device that can prevent fat cells from being crushed and facilitate liquid replacement is urgently needed. Utility Model Content

[0006] The utility model discloses a kind of slide glass components for adipocyte experiment, to partially alleviate or solve the above problems, prevent adipocyte from separating from slide glass platform during culture or liquid change process.

[0007] To solve the above-mentioned technical problems, the utility model specifically adopts the following technical solutions:

[0008] A kind of slide glass component for adipocyte experiment, including slide glass platform, the first surface of the slide glass platform is provided with experimental area, the experimental area is provided with containing groove, the third cover glass corresponding with the containing groove is further provided on the slide glass platform, the third cover glass and the containing groove are collectively enclosed to form containing space for containing the adipocyte, containing groove opposite two sides are respectively provided with liquid injection channel and liquid suction channel;

[0009] Among them, the containing groove is enclosed by the first cover glass and the second cover glass which are located on the first surface of the slide glass platform and symmetrically arranged on the two sides of the experimental area, and the notch between the first cover glass and the second cover glass forms the liquid injection channel and the liquid suction channel respectively;Or,

[0010] The containing groove is formed by the recess provided in the experimental area, and the drainage channels in the two side walls of the recess are connected to the first surface of the slide glass platform to form the liquid injection channel and the liquid suction channel respectively.

[0011] As an improvement, the first surface of the slide glass platform is provided with first mounting area and second mounting area, the first cover glass and the second cover glass are arranged in the first mounting area and the second mounting area respectively, and the experimental area is located between the first mounting area and the second mounting area;The two sides of the third cover glass are arranged on the first cover glass and the second cover glass respectively, and the middle part of the third cover glass corresponds to the experimental area and forms the containing space with the containing groove;

[0012] The two side walls corresponding to the experimental area of the third cover glass are respectively provided with first baffle and second baffle, the height of the first baffle and the second baffle is less than the thickness of the first cover glass and the second cover glass, so that the two sides of the containing space form the liquid injection channel and the liquid suction channel respectively.

[0013] As an improvement, the first end of the liquid suction channel and the liquid injection channel is arranged at the bottom of the containing groove respectively, and the second end of the liquid suction channel and the liquid injection channel is arranged on the first surface of the slide glass platform respectively and located on the two sides of the third cover glass.

[0014] As an improvement, the first cover glass and the second cover glass are respectively matched with the positioning plate, and the first cover glass and the second cover glass are respectively matched with the first mounting area and the second mounting area when the first cover glass and the second cover glass are respectively matched with the positioning plate.

[0015] As an improvement, the positioning plate is in a concave shape; the first cover glass and the second cover glass are respectively matched with the first mounting area and the second mounting area when the first cover glass and the second cover glass are placed into the concave groove of the positioning plate and respectively abut against the first corner and the second corner of the positioning plate.

[0016] As an improvement, the first baffle and the second baffle are formed by extending the two side walls of the third cover glass towards the first surface.

[0017] As an improvement, the first baffle and the second baffle are respectively bonded on the two side walls of the third cover glass.

[0018] As an improvement, the thicknesses of the first cover glass, the second cover glass and the third cover glass are the same, and the heights of the first baffle and the second baffle are the same and less than the thickness of the first cover glass.

[0019] As an improvement, the inner diameters of the liquid injection channel and the liquid suction channel gradually increase from the first end to the second end.

[0020] As an improvement, the positioning plate for positioning the third cover glass is further included, and the third cover glass is matched with the positioning plate to form the accommodation space corresponding to the accommodation groove.

[0021] The principle and beneficial technical effects of the utility model are as follows:

[0022] The utility model comprehensively provides a quick construction type anti-loss monolayer fat cell special slide glass assembly.

[0023] Specifically, the accommodation space for accommodating fat cells is built on the slide glass table, and the three cover glasses and the two baffles on the third cover glass are matched with each other, so that the accommodation space has the liquid injection channel and the liquid suction channel at the bottom of the accommodation space; or the accommodation groove is pre-set on the slide glass table, and the liquid injection channel and the liquid suction channel are arranged at the bottom of the accommodation groove; and the two channels are oppositely arranged, and the culture solution flows from one side to the other side of the accommodation space when the liquid is changed, so that sufficient liquid change is realized without taking away the fat cells floating above the culture solution, and since the height of the accommodation space is constant and slightly greater than the height of the added liquid, the pressure applied on the fat cells due to the gravity of the cover glass is greatly reduced, so that the fat cells will not be crushed even after the cover glass is placed.

[0024] Furthermore, the three cover glasses are designed to be assembled. During use, the first and second cover glasses are quickly positioned using the positioning plate, and then the third cover glass is installed. Alternatively, the entire slide assembly can be installed by simply positioning the third cover glass using the positioning plate, making it quick and easy to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for the embodiments or the description of the prior art. In all drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the various elements or parts are not necessarily drawn according to the actual scale. Obviously, the drawings described below are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without inventive work.

[0026] Figure 1 This is a schematic diagram of the installation structure of a glass slide assembly according to an exemplary embodiment of the present invention;

[0027] Figure 2 This is a schematic diagram of a glass slide assembly according to an exemplary embodiment of the present invention after installation;

[0028] Figure 3 An exploded view of an exemplary embodiment of the present invention showing a glass slide assembly during installation;

[0029] Figure 4 This is a partial structural diagram of a glass slide assembly after installation according to an exemplary embodiment of the present invention;

[0030] Figure 5 A side cross-sectional view of a slide assembly after installation of an exemplary embodiment of the present invention;

[0031] Figure 6 is a cross-sectional view of a slide assembly in another embodiment of the present invention;

[0032] Figure 7 A cross-sectional view of a glass slide assembly at another angle in another embodiment of the present invention;

[0033] Figure 8 This is a schematic diagram of the overall structure of a glass slide assembly in another embodiment of the present invention;

[0034] Figure 9 This is an exploded view of a glass slide assembly in another embodiment of the present invention.

[0035] Markings in the figure: 1, glass slide; 101, receiving groove; 2, first cover glass; 3, second cover glass; 4, third cover glass; 41, first baffle; 42, second baffle; 5, positioning plate; 6, liquid suction channel; 7, liquid injection channel. DETAILED DESCRIPTION

[0036] To make the purpose, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0037] In this document, the use of suffixes such as "module", "component" or "unit" to indicate elements is only for the purpose of facilitating the description of the present invention and has no specific meaning in itself. Therefore, "module", "component" or "unit" can be used interchangeably. In this document, the orientation or positional relationship indicated by the terms "upper", "lower", "inside", "outside", "front", "back", "one end", "the other end", etc. is based on the orientation or positional relationship shown in the accompanying drawings and is only for the convenience of describing the present invention and simplifying the description. It does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0038] As used herein, unless otherwise expressly specified or limited, the terms "installed," "provided with," "connected," etc., should be understood broadly. For example, "connected" can mean a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, a direct connection, an indirect connection through an intermediate medium, or internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in this utility model. As used herein, "plurality" means two or more, including two, three, four, five, etc.

[0039] Example 1

[0040] This embodiment is basically as Figure 1-Figure 5 As shown:

[0041] like Figure 1The present invention provides a glass slide assembly for fat cell experiments, comprising a glass slide 1, wherein a first surface (upper surface) of the glass slide 1 is provided with an experimental area, wherein the experimental area is provided with a receiving groove, and a third cover glass 4 corresponding to the receiving groove is further provided on the glass slide 1, wherein the third cover glass 4 and the receiving groove together enclose a receiving space for receiving the fat cells, and an injection channel 7 and a suction channel 6 are respectively provided on opposite sides of the receiving groove;

[0042] In which, the receiving groove is formed by the first cover glass 2 and the second cover glass 3 located on the first surface of the glass slide 1 and symmetrically arranged on both sides of the experimental area, and the injection channel 7 and the aspiration channel 6 are formed at the gap between the first cover glass 2 and the second cover glass 3 respectively; specifically, the two adjacent side walls of the first cover glass 2 and the second cover glass 3 form a groove with the experimental area, and the two opposite open ends of the groove are the gap.

[0043] In some embodiments, the first surface of the glass slide 1 is provided with a first mounting area and a second mounting area, the first cover glass 2 and the second cover glass 3 are respectively provided in the first mounting area and the second mounting area, and the experimental area is located between the first mounting area and the second mounting area; the two sides of the third cover glass 4 are respectively provided on the first cover glass 2 and the second cover glass 3, and the middle portion of the third cover glass 4 corresponds to the experimental area and forms the accommodation space together with the accommodation groove;

[0044] The two side walls of the third cover glass 4 corresponding to the experimental area are respectively provided with a first baffle 41 and a second baffle 42. The heights of the first baffle 41 and the second baffle 42 are both less than the thicknesses of the first cover glass 2 and the second cover glass 3, so that the injection channel 7 and the suction channel 6 are respectively formed on both sides of the accommodating space.

[0045] The specific structure of the slide assembly can be found in Figure 5 The first cover glass 2, the second cover glass 3 and the third cover glass 4 are all in the shape of a cuboid, and the accommodating space as a whole is also in the shape of a cuboid with an open bottom.

[0046] By providing the injection channel 7 and the suction channel 6 located at the bottom of the accommodating space and arranged opposite to each other, the culture medium flows from one side of the accommodating space to the other side when the medium is replaced, thereby achieving sufficient liquid replacement without taking away the fat cells floating on the culture medium, and effectively preventing the fat cells from detaching from the glass slide 1 during the culture or liquid replacement process.

[0047] In some embodiments, the first cover glass 2, the second cover glass 3 and the third cover glass 4 have the same thickness, the first baffle 41 and the second baffle 42 have the same height and are less than the thickness of the first cover glass 2, and the length of the first baffle 41 and the second baffle 42 is the same as the length of the receiving groove (or the length of the gap).

[0048] In some embodiments, a positioning plate 5 is further included. When the first cover glass 2 and the second cover glass 3 are respectively matched with the positioning plate 5, the first cover glass 2 and the second cover glass 3 correspond to the first installation area and the second installation area respectively.

[0049] In some embodiments, the positioning plate 5 is concave in shape; when the first cover glass 2 and the second cover glass 3 are placed in the groove of the positioning plate 5 and respectively rest against the first corner and the second corner of the positioning plate 5, the first cover glass 2 and the second cover glass 3 correspond to the first installation area and the second installation area, respectively; wherein the first corner and the second corner refer to the bending angles formed at the connection between the two side edges and the bottom edge of the positioning plate 5.

[0050] In some embodiments, the first surface of the glass slide is further provided with positioning buckles or marked with positioning marks for quickly determining the position of the positioning plate.

[0051] By setting a concave-shaped positioning plate, when in use, first place the positioning plate at a designated position on the slide table, then slide the first cover glass and the second cover glass from the opening of the concave-shaped positioning plate into the groove thereof and respectively rest against the first corner and the second corner. At this time, a receiving cavity is formed, into which a culture medium containing fat cells can be dripped, and then the third cover glass is installed. Specifically, the two sides of the third cover glass are respectively placed on the upper surfaces of the first cover glass and the second cover glass, and the first baffle and the second baffle are respectively located at the two notches. At this time, the third cover glass cooperates with the receiving groove to form a receiving space; finally, the positioning plate is removed, and the slide assembly is completed.

[0052] In some embodiments, the first baffle and the second baffle are formed by extending two side walls of the third cover glass toward the first surface.

[0053] In some other embodiments, the first baffle and the second baffle are respectively adhered to two side walls of the third cover glass.

[0054] It should be noted that the first cover glass, the second cover glass, the third cover glass and the positioning plate can be connected to the glass slide in a "placement" manner. Such a setting can make the construction and disassembly of each component more convenient.

[0055] Example 2

[0056] See also Figure 6-Figure 9 The present invention provides a glass slide assembly for fat cell experiments, comprising a glass slide 1, an experimental area provided on the glass slide 1, a receiving groove 101 provided on the experimental area, a third cover glass 4 corresponding to the receiving groove 101, the third cover glass 4 and the receiving groove 101 together enclose a receiving space for receiving the fat cells, and an injection channel 7 and a suction channel 6 are respectively provided on opposite sides of the receiving groove 101; wherein, the receiving groove 101 is formed as a depression provided in the experimental area, and the drainage channels on both side walls of the depression connected to the first surface of the glass slide 1 form the injection channel 7 and the suction channel 6 respectively.

[0057] In some embodiments, the first ends of the liquid aspiration channel 6 and the liquid injection channel 7 are respectively arranged at the bottom of the side wall of the receiving tank 101, and the second ends of the liquid aspiration channel 6 and the liquid injection channel 7 are respectively arranged on the first surface of the glass slide 1 and are respectively located on both sides of the third cover glass 4.

[0058] In some embodiments, the inner diameters of the injection channel 7 and the aspiration channel 6 gradually increase from the first end to the second end, so that the operating ends (second ends) of the injection channel 7 and the aspiration channel 6 have larger operating surfaces, which facilitates operation. At the same time, the openings of the liquid inlet end and the liquid outlet end (first end) are small, which can effectively prevent fat cells from flowing out along with the culture medium.

[0059] In some embodiments, a positioning plate for positioning the third cover glass 4 is further included. When the third cover glass 4 is matched with the positioning plate, the third cover glass 4 and the receiving groove 101 correspond to form the receiving space.

[0060] During specific use, first drip the culture fluid containing fat cells into the receiving tank 101, then place the positioning plate at the designated position next to the receiving tank 101, and then insert the third cover glass 4 into the positioning plate so that the third cover glass 4 covers the top opening of the receiving tank 101. The third cover glass 4 and the receiving tank 101 form a relatively closed receiving space, and then remove the positioning plate. During the subsequent culture process, the culture fluid can be injected into the receiving space through the injection channel 7, and the fluid can be aspirated simultaneously through the aspiration channel 6 to achieve fluid replacement.

[0061] In summary, the present application comprehensively provides a quick-build, loss-proof, and dedicated glass slide assembly for monolayered fat cells.

[0062] It is to be understood that the terminology "including", "comprising", or any other variation thereof, is intended to cover a non-exclusive inclusion such that processes, methods, articles, or apparatuses that comprise a list of elements are not necessarily limited to those elements, but can include other elements not expressly listed or inherent to such processes, methods, articles, or apparatuses. Without further limitation, an element preceded by "comprises a" does not, without more limitations, foreclose the existence of additional identical elements in the process, method, article, or apparatus that comprises the recited element.

[0063] The embodiments of the present application are described above in conjunction with the drawings, but the present application is not limited to the specific embodiments described above, and the specific embodiments described above are merely illustrative rather than restrictive, and a person of ordinary skill in the art can make many forms under the inspiration of the present application without departing from the purpose of the present application and the scope of protection of the claims, which are all within the protection of the present application.

Claims

1. A glass slide assembly for fat cell experiments, characterized by: The device comprises a glass slide, wherein a first surface of the glass slide is provided with an experimental area, the experimental area is provided with a receiving groove, a third cover glass is further provided on the glass slide corresponding to the receiving groove, the third cover glass and the receiving groove together enclose a receiving space for receiving the fat cells, and an injection channel and a suction channel are respectively provided on opposite sides of the receiving groove; The receiving tank is formed by a first cover glass and a second cover glass located on the first surface of the glass slide and symmetrically arranged on both sides of the experimental area, and the experimental area is enclosed by the first cover glass and the second cover glass, and the injection channel and the aspiration channel are formed at the gap between the first cover glass and the second cover glass respectively; or The receiving groove is formed as a depression arranged in the experimental area, and the drainage channels in both side walls of the depression are connected to the first surface of the glass stage to form the injection channel and the suction channel respectively.

2. The glass slide assembly for fat cell experiments according to claim 1, characterized in that: The first surface of the glass slide is provided with a first mounting area and a second mounting area, the first cover glass and the second cover glass are respectively provided in the first mounting area and the second mounting area, and the experimental area is located between the first mounting area and the second mounting area; two sides of the third cover glass are respectively provided on the first cover glass and the second cover glass, and the middle portion of the third cover glass corresponds to the experimental area and forms the accommodation space together with the accommodation groove; The two side walls of the third cover glass corresponding to the experimental area are respectively provided with a first baffle and a second baffle, and the heights of the first baffle and the second baffle are both less than the thicknesses of the first cover glass and the second cover glass, so that the injection channel and the suction channel are respectively formed on both sides of the accommodating space.

3. The glass slide assembly for fat cell experiments according to claim 1, characterized in that: The first ends of the liquid aspiration channel and the liquid injection channel are respectively arranged at the bottom of the receiving tank, and the second ends of the liquid aspiration channel and the liquid injection channel are respectively arranged on the first surface of the glass stage and are respectively located on both sides of the third cover glass.

4. The glass slide assembly for fat cell experiments according to claim 2, characterized in that: A positioning plate is also included. When the first cover glass and the second cover glass are respectively matched with the positioning plate, the first cover glass and the second cover glass respectively correspond to the first installation area and the second installation area.

5. The glass slide assembly for fat cell experiments according to claim 4, characterized in that: The positioning plate is concave in shape; when the first cover glass and the second cover glass are placed in the groove of the positioning plate and respectively rest against the first corner and the second corner of the positioning plate, the first cover glass and the second cover glass correspond to the first installation area and the second installation area respectively.

6. A glass slide assembly for fat cell experiments according to any one of claims 2, 4, and 5, characterized in that: The first baffle and the second baffle are formed by extending two side walls of the third cover glass toward the first surface.

7. A glass slide assembly for fat cell experiments according to any one of claims 2, 4, and 5, characterized in that: The first baffle and the second baffle are respectively adhered to two side walls of the third cover glass.

8. The glass slide assembly for fat cell experiments according to claim 2, characterized in that: The first cover glass, the second cover glass, and the third cover glass have the same thickness, and the first baffle and the second baffle have the same height and are smaller than the thickness of the first cover glass.

9. The glass slide assembly for fat cell experiments according to claim 3, characterized in that: The inner diameters of the liquid injection channel and the liquid suction channel gradually increase from the first end to the second end.

10. The glass slide assembly for fat cell experiments according to claim 3, characterized in that: It also includes a positioning plate for positioning the third cover glass. When the third cover glass is matched with the positioning plate, the third cover glass and the receiving groove correspond to form the receiving space.

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