Magnetic bead coating equipment for antibody magnetic beads

Abstract

The utility model discloses magnetic bead coating equipment for antibody magnetic beads, which relates to the technical field of magnetic bead coating equipment and comprises a worktable, magnetic stirring equipment is mounted on the top surface of the worktable, a placing disc is arranged on the top surface of the magnetic stirring equipment, a rotating plate is rotatably arranged in the worktable, and a rotating shaft is arranged on the rotating plate. A first positioning rod is fixedly arranged on the top face of the rotating plate, the outer surface of the first positioning rod is rotationally sleeved with a guide plate, a moving plate is oppositely arranged on the top face of the workbench in a sliding mode, a clamping piece is arranged on one side of the moving plate, and through rotation of the rotating plate, one end of the guide plate is driven to rotate on the outer surface of the first positioning rod; and the other end of the rotating shaft rotates on the outer surface of the second positioning rod, so that the two sliding blocks are promoted to move mutually, then the two moving plates move mutually, the clamping piece clamps the cup body, and the cup body is centered on the placing disc, so that in the stirring process, the cup body does not displace, and the stirring speed is increased.

Description

Technical Field

[0001] The utility model relates to the technical field of magnetic bead coating equipment, in particular to a magnetic bead coating equipment for antibody magnetic beads. Background Art

[0002] Antibody magnetic beads are a tool used for antibody immunoaffinity purification. They play an important role in the field of immunology research and can be used for antibody screening, enrichment, expression product purification, antibody library construction and screening, and detection and enrichment of cytokines and growth factors.

[0003] When magnetic beads are bound to antibodies, they need to be mixed and coated using a magnetic stirring device to ensure that the magnetic beads are evenly distributed in the buffer solution, thereby improving the binding efficiency and purity. However, when existing magnetic stirring devices are used to suspend and coat the magnetic beads, the cup body is prone to displacement due to shaking during the stirring process, resulting in a decrease in the magnetic stirring speed. To address the above problems, the inventors have proposed a magnetic bead coating device for antibody magnetic beads to solve the above problems. Utility Model Content

[0004] In order to solve the problem that when a magnetic stirring device is used to coat magnetic beads, the cup body is easily displaced due to shaking during the stirring process, resulting in a decrease in the magnetic stirring speed; the purpose of the utility model is to provide a magnetic bead coating device for antibody magnetic beads.

[0005] In order to solve the above technical problems, the utility model adopts the following technical solutions: a magnetic bead coating device for antibody magnetic beads, comprising a workbench, a magnetic stirring device is installed on the top surface of the workbench, a placing disk is provided on the top surface of the magnetic stirring device, a rotating plate is rotatably provided in the workbench, a first positioning rod is fixed on the top surface of the rotating plate, a guide plate is rotatably sleeved on the outer surface of the first positioning rod, a movable plate is slidably provided on the top surface of the workbench, a clamping piece is provided on one side of the movable plate, a slider is fixed on the bottom surface of the movable plate, the slider is slidably arranged in the workbench, a moving block is fixed on one side of the slider, a second positioning rod is fixed on the top surface of the moving block, an end of the guide plate away from the first positioning rod is rotatably sleeved on the outer surface of the second positioning rod, an extension rod is fixed on one side of the movable plate, and the clamping piece is fixed on one side of the extension rod On the other hand, first place the cup containing magnetic beads, antibodies and buffer on the placement plate, then place the stirrer in the cup, and then turn on the first motor to rotate the rotating shaft, and then the rotating shaft drives the worm to rotate, the worm is engaged with the worm gear, so that the rotating rod rotates in the workbench, and drives the rotating plate to rotate, prompting one end of the guide plate to rotate on the outer surface of the first positioning rod, and the other end thereof rotates on the outer surface of the second positioning rod, prompting the two sliders to move relative to each other, and then the two moving plates move relative to each other, so that the clamping part clamps the cup and centers it on the placement plate, so that the cup will not be displaced during the stirring process, thereby increasing the stirring speed, and by turning on the second motor to rotate the threaded rod, the threaded block drives the cover plate to move down, so that the cover plate can fit on the top surface of the cup body and is sealed, and then the magnetic stirring device is turned on to allow the stirrer to stir the cup body.

[0006] Preferably, a rotating rod is rotatably inserted in the workbench, the rotating plate is fixedly sleeved on the outer surface of the rotating rod, a first motor is installed on one side of the workbench, a rotating shaft is fixedly provided at the output end of the first motor, the rotating shaft is rotatably inserted in the workbench, a worm is fixedly provided at one end of the rotating shaft, a worm wheel is fixedly sleeved on the outer surface of the rotating rod, and the worm is meshed with the worm wheel.

[0007] Preferably, a support plate is fixedly provided on the top surface of the workbench, a cover plate is slidably provided on one side of the support plate, a sealing plate is fixedly provided on the bottom surface of the cover plate, a threaded block is fixedly provided on one side of the cover plate, the threaded block is slidably provided in the support plate, a second motor is installed on the top surface of the support plate, a threaded rod is fixedly provided on the output end of the second motor, and the threaded block is threadedly sleeved on the outer surface of the threaded rod.

[0008] Compared with the prior art, the beneficial effects of the present invention are:

[0009] 1. In the present invention, the rotating plate rotates, causing one end of the guide plate to rotate on the outer surface of the first positioning rod, and the other end of the guide plate to rotate on the outer surface of the second positioning rod, thereby pulling the two sliders to move relative to each other, and then the two movable plates move relative to each other, so that the clamping member clamps the cup body and centers it on the placement plate. Therefore, during the stirring process, the cup body will not be displaced, thereby improving the stirring speed;

[0010] 2. In the present invention, by turning on the second motor, the threaded rod rotates, prompting the threaded block to drive the cover plate downward, so that the cover plate can fit on the top surface of the cup body and seal it. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0012] Figure 1 This is a schematic diagram of the overall structure of the utility model;

[0013] Figure 2 This is a partial cross-sectional structural diagram of the workbench of the utility model;

[0014] Figure 3 This is a schematic diagram of the first motor structure of the utility model;

[0015] Figure 4 This is a schematic diagram of a partial cross-sectional structure of the support plate of the utility model;

[0016] Figure 5 For this utility model Figure 2 Enlarged structural diagram at point A in the middle.

[0017] In the figure: 1. workbench; 11. magnetic stirring device; 12. placement plate; 2. first motor; 21. rotating shaft; 22. worm; 23. rotating rod; 24. worm gear; 25. rotating plate; 251. first positioning rod; 26. guide plate; 27. moving block; 271. second positioning rod; 28. slider; 29. ​​moving plate; 291. extension rod; 292. clamping member; 3. support plate; 31. second motor; 32. threaded rod; 33. threaded block; 34. cover plate; 35. sealing plate. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0019] Example: Figure 1-5 As shown, the utility model provides a magnetic bead coating device for antibody magnetic beads, including a workbench 1, a magnetic stirring device 11 is installed on the top surface of the workbench 1, a placement plate 12 is provided on the top surface of the magnetic stirring device 11, a rotating plate 25 is rotatably provided in the workbench 1, a first positioning rod 251 is fixedly provided on the top surface of the rotating plate 25, a guide plate 26 is rotatably sleeved on the outer surface of the first positioning rod 251, a movable plate 29 is provided on the top surface of the workbench 1 for sliding toward each other, a clamping member 292 is provided on one side of the movable plate 29, a slider 28 is fixed on the bottom surface of the movable plate 29, the slider 28 is slidably provided in the workbench 1, a movable block 27 is fixed on one side of the slider 28, and the top surface of the movable block 27 is fixed A second positioning rod 271 is provided, and one end of the guide plate 26 away from the first positioning rod 251 is rotatably sleeved on the outer surface of the second positioning rod 271. An extension rod 291 is fixedly provided on one side of the movable plate 29, and a clamping member 292 is fixedly provided on one side of the extension rod 291. By rotating the rotating plate 25, one end of the guide plate 26 is rotated on the outer surface of the first positioning rod 251, and the other end is rotated on the outer surface of the second positioning rod 271, prompting the two sliders 28 to move relative to each other, and then the two movable plates 29 move relative to each other, so that the clamping member 292 clamps the cup body and centers it on the placement plate 12, so that the cup body will not be displaced during the stirring process, thereby improving the stirring speed.

[0020] A rotating rod 23 is rotatably inserted in the workbench 1, and a rotating plate 25 is fixedly sleeved on the outer surface of the rotating rod 23. A first motor 2 is installed on one side of the workbench 1, and a rotating shaft 21 is fixedly provided at the output end of the first motor 2. The rotating shaft 21 is rotatably inserted in the workbench 1, and a worm 22 is fixedly provided at one end of the rotating shaft 21. A worm wheel 24 is fixedly sleeved on the outer surface of the rotating rod 23, and the worm 22 is meshed with the worm wheel 24.

[0021] By adopting the above technical solution, by turning on the first motor 2, the rotating shaft 21 rotates, and then the rotating shaft 21 drives the worm 22 to rotate, and the worm 22 engages with the worm wheel 24, so that the rotating rod 23 rotates in the workbench 1 and drives the rotating plate 25 to rotate, thereby adjusting the distance between the two movable plates 29.

[0022] A support plate 3 is fixed on the top surface of the workbench 1, a cover plate 34 is slidably provided on one side of the support plate 3, a sealing plate 35 is fixed on the bottom surface of the cover plate 34, a threaded block 33 is fixed on one side of the cover plate 34, the threaded block 33 is slidably provided in the support plate 3, a second motor 31 is installed on the top surface of the support plate 3, a threaded rod 32 is fixed on the output end of the second motor 31, and the threaded block 33 is threadedly sleeved on the outer surface of the threaded rod 32.

[0023] By adopting the above technical solution, the second motor 31 is turned on to rotate the threaded rod 32, prompting the threaded block 33 to drive the cover plate 34 to move downward, so that the cover plate 34 can fit on the top surface of the cup body and seal it.

[0024] Working principle: First, place the cup containing magnetic beads, antibodies and buffer on the placement tray 12, then place the stirrer in the cup, and then turn on the first motor 2, so that the rotating shaft 21 rotates, and then the rotating shaft 21 drives the worm 22 to rotate, and the worm 22 engages with the worm wheel 24, so that the rotating rod 23 rotates in the workbench 1, and drives the rotating plate 25 to rotate, prompting one end of the guide plate 26 to rotate on the outer surface of the first positioning rod 251, and its other end to rotate on the outer surface of the second positioning rod 271, prompting the two sliders 28 to move relative to each other, and then the two moving plates 29 move relative to each other, so that the clamping member 292 clamps the cup and centers it on the placement tray 12, so that the cup will not be displaced during the stirring process, thereby increasing the stirring speed, by turning on the second motor 31, so that the threaded rod 32 rotates, prompting the threaded block 33 to drive the cover plate 34 downward, so that the cover plate 34 can fit on the top surface of the cup and seal it, and then turn on the magnetic stirring device 11, so that the stirrer stirs the cup.

[0025] Obviously, those skilled in the art may make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include such modifications and variations.

Claims

1. A magnetic bead coating device for antibody magnetic beads, comprising a workbench (1), characterized in that: A magnetic stirring device (11) is installed on the top surface of the workbench (1), and a placement plate (12) is provided on the top surface of the magnetic stirring device (11). A rotating plate (25) is rotatably provided in the workbench (1), and a first positioning rod (251) is fixedly provided on the top surface of the rotating plate (25). A guide plate (26) is rotatably sleeved on the outer surface of the first positioning rod (251). A movable plate (29) is slidably provided on the top surface of the workbench (1), and a clamping member (292) is provided on one side of the movable plate (29). A slider (28) is fixedly provided on the bottom surface of the movable plate (29), and the slider (28) is slidably provided in the workbench (1). A movable block (27) is fixedly provided on one side of the slider (28), and a second positioning rod (271) is fixedly provided on the top surface of the movable block (27). An end of the guide plate (26) away from the first positioning rod (251) is rotatably sleeved on the outer surface of the second positioning rod (271).

2. The magnetic bead coating device for antibody magnetic beads according to claim 1, characterized in that: An extension rod (291) is fixedly provided on one side of the movable plate (29), and the clamping member (292) is fixedly provided on one side of the extension rod (291).

3. The magnetic bead coating device for antibody magnetic beads according to claim 1, characterized in that: A rotating rod (23) is rotatably inserted in the workbench (1), and the rotating plate (25) is fixedly sleeved on the outer surface of the rotating rod (23).

4. The magnetic bead coating device for antibody magnetic beads according to claim 3, characterized in that: A first motor (2) is installed on one side of the workbench (1); a rotating shaft (21) is fixedly provided at the output end of the first motor (2); and the rotating shaft (21) is rotatably inserted into the workbench (1).

5. The magnetic bead coating device for antibody magnetic beads according to claim 4, characterized in that: A worm (22) is fixedly provided at one end of the rotating shaft (21), a worm wheel (24) is fixedly provided on the outer surface of the rotating rod (23), and the worm (22) is meshed with the worm wheel (24).

6. The magnetic bead coating device for antibody magnetic beads according to claim 1, characterized in that: A support plate (3) is fixedly provided on the top surface of the workbench (1), a cover plate (34) is slidably provided on one side of the support plate (3), and a sealing plate (35) is fixedly provided on the bottom surface of the cover plate (34).

7. The magnetic bead coating device for antibody magnetic beads according to claim 6, characterized in that: A threaded block (33) is fixedly provided on one side of the cover plate (34), and the threaded block (33) is slidably arranged in the support plate (3).

8. The magnetic bead coating device for antibody magnetic beads according to claim 7, characterized in that: A second motor (31) is mounted on the top surface of the support plate (3), a threaded rod (32) is fixedly provided at the output end of the second motor (31), and the threaded block (33) is threadedly sleeved on the outer surface of the threaded rod (32).

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