Disposable platelet-rich plasma separating device

Abstract

The utility model belongs to the technical field of plasma separation devices, and relates to a disposable platelet-rich plasma separation device which comprises a cylinder body, a lifting pipe, an insertion rod and a screw cap, the lifting pipe is inserted into the cylinder body, and the screw cap rotates to drive the lifting pipe to do lifting motion; the lower end of the lifting pipe is sealed, a through hole is formed in the pipe wall close to the lower end, and the diameter of the through hole is 0.5-3 mm; when the lifting pipe moves downwards to the limit, the liquid storage cavity in the barrel body is just divided into an upper part and a lower part by the lifting pipe; when the inserting rod is inserted into the lifting pipe and moves downwards to the limit, the through hole is just sealed by the lifting pipe. According to the device disclosed by the utility model, through the secondary centrifugal design without replacing the centrifugal tube, the PRP concentration is effectively improved, meanwhile, the pollution risk in the preparation process is reduced, and the preparation efficiency and the safety are improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of plasma separation device, and relates to a disposable platelet-rich plasma separation device. BACKGROUND

[0002] Platelet-rich plasma (PRP) is a highly concentrated platelet preparation extracted from autologous blood through centrifugation technology. Activated platelets can release a series of growth factors, including platelet-derived growth factor, insulin-like growth factor, vascular endothelial growth factor, and epidermal growth factor, which play a crucial role in promoting tissue cell regeneration and repair. In view of the advantages of PRP, such as convenient source, no autologous immune rejection, and simple preparation, it has been widely used in orthopedics, dentistry, oral and maxillofacial surgery, plastic and cosmetic surgery, and other medical fields in the past two decades.

[0003] However, although PRP has achieved remarkable results in clinical applications, its preparation methods show diversification and individualization. Currently, the centrifugation method is the mainstream method for preparing PRP, and the centrifugation methods are mainly divided into single centrifugation and double centrifugation. Different centrifugation methods directly affect the concentration of platelets in the final product, with a concentration range from 13 times to 48 times, or even higher. Studies have shown that compared with single centrifugation, double centrifugation can more effectively increase the concentration of platelets, therefore, the current trend is to use double centrifugation to prepare PRP.

[0004] In the process of preparing PRP by double centrifugation, the extracted blood needs to be subjected to single centrifugation to separate red blood cells, PRP, and platelet-poor plasma (PPP). Subsequently, the separated PRP and PPP layer are transferred together to a new centrifuge tube for double centrifugation. After this process is completed, PRP with high concentration can be obtained. However, during the transfer process, PRP and PPP layer will inevitably come into contact with air, which increases the risk of contamination of plasma and may be accompanied by a certain degree of plasma loss.

[0005] Patent CN216573622U discloses a disposable platelet-rich plasma separation device, which aims to solve the problems of blood leakage and insufficient sealing in the preparation process of the prior art. Through the ingenious combination of its unique design, such as the upper cover, lifting tube, and rotating cover, the device achieves the preliminary separation of blood. However, the device is currently mainly suitable for single centrifugation operation, and for double centrifugation method requiring higher platelet concentration, the centrifuge tube still needs to be replaced for further concentration of platelets. This not only increases the complexity of the operation steps, but also may introduce new contamination risks.

[0006] Therefore, in order to improve the preparation efficiency and reduce the pollution risk, it is necessary to develop a new type of disposable platelet-rich plasma separation device. The device should be designed to complete the secondary centrifugation process without replacing the centrifuge tube, so as to directly obtain high concentration PRP. Utility model content

[0007] The utility model aims at solving the prior art problems, provide a kind of disposable platelet-rich plasma separation device.

[0008] To achieve the above object, the technical scheme adopted by the utility model is as follows:

[0009] A kind of disposable platelet-rich plasma separation device, including barrel, lifting pipe and screw cap, lifting pipe is inserted into barrel, screw cap rotates and drives lifting pipe to make lifting motion, it further includes plug rod;The lower end of lifting pipe is sealed, and the pipe wall close to the lower end is provided with through hole, the diameter of through hole is 0.5-3mm, if the diameter of through hole is too small, then the PRP with poor flowability is difficult to enter lifting pipe through through hole, if the diameter of through hole is too large, then the liquid flowing into lifting pipe is not controlled, and the concentration of PRP in the final product will be reduced;When lifting pipe moves downward to limit, the liquid storage cavity in barrel is just divided into upper and lower two parts by lifting pipe;Plug rod is inserted into lifting pipe, and when plug rod moves downward to limit, through hole is just closed by lifting pipe.

[0010] As a preferred technical scheme:

[0011] As described above, a kind of disposable platelet-rich plasma separation device, the number of through hole is 2 or 4, and is uniformly distributed around the central axis of lifting pipe, since the flowability of PRP is poor, therefore, the uniform distribution of through hole can make PRP be uniformly sucked into lifting pipe under the action of negative pressure, and then enter into syringe.

[0012] As described above, a kind of disposable platelet-rich plasma separation device, the distance between through hole and the lower end of lifting pipe is 2.5-5mm, so that when lifting pipe moves downward to limit, the lowermost end of through hole is still in upper liquid storage cavity, the sealing property of lifting pipe is good, can avoid that PPP in upper liquid storage cavity and PRP after preliminary centrifugation are thrown into lower liquid storage cavity under the action of centrifugal force during secondary centrifugation process, and at the same time, PPP in upper layer after secondary centrifugation can be avoided to enter into lifting pipe, resulting in that the concentration of PRP in product after secondary centrifugation is lower.

[0013] A disposable platelet-rich plasma separation device as described above, the barrel body is sequentially connected by coaxial and equal wall thickness upper hollow cylinder, upper hollow inverted circular table, middle hollow cylinder, lower hollow circular table and lower hollow cylinder; the large end of the upper hollow inverted circular table is upward and the small end is downward, the outer diameter of the large end is the same as that of the upper hollow cylinder, and the outer diameter of the small end is the same as that of the middle hollow cylinder; the small end of the lower hollow circular table is upward and the large end is downward, the outer diameter of the small end is the same as that of the middle hollow cylinder, and the outer diameter of the large end is the same as that of the lower hollow cylinder; the outer diameter of the lifting pipe is greater than the inner diameter of the middle hollow cylinder, so that the lifting pipe can be squeezed into the middle hollow cylinder during assembly to form an interference fit.

[0014] A disposable platelet-rich plasma separation device as described above, further comprising an upper cover, an upper cover sealing element and a screw cover sealing element;

[0015] The upper cover is screwed and locked with the top end of the barrel body; the screw cover is located above the upper cover and is rotatably connected with the upper cover and covers only part of the area of the upper cover; rotation refers to movement around a circular trajectory, and the center of the circular trajectory is located on the central axis of the upper cover;

[0016] An injection hole is provided on the upper cover, and a suction hole is provided on the screw cover; the upper cover sealing element is used to close the injection hole, and the screw cover sealing element is used to close the suction hole;

[0017] The screw cover extends downward from the hole wall of the suction hole to form a screw cover inner tube, and the upper cover extends downward from the hole wall of the injection hole to form an upper cover inner tube;

[0018] Two symmetrical protrusions are provided on the outer tube wall of the lifting pipe; two symmetrical vertical clamping grooves are provided on the tube wall of the screw cover inner tube; two symmetrical inclined grooves are provided on the tube wall of the upper cover inner tube, and the slotting range of the inclined grooves is 180°;

[0019] The screw cover inner tube penetrates into the upper cover inner tube, the upper end of the lifting pipe penetrates into the screw cover inner tube, the two protrusions penetrate into the two inclined grooves from the two vertical clamping grooves respectively, and the two inclined grooves are used to constrain the two protrusions of the lifting pipe to slide up and down during rotation.

[0020] A disposable platelet-rich plasma separation device as described above, the tube wall of the screw cover inner tube is further provided with two symmetrical upper horizontal clamping grooves and two symmetrical lower horizontal clamping grooves, and the two vertical clamping grooves, the two upper horizontal clamping grooves and the two lower horizontal clamping grooves are connected to form two Z-shaped clamping grooves.

[0021] A disposable platelet-rich plasma separation device as described above, the installation position of the screw cover and the upper cover sealing element satisfies that the upper cover sealing element is completely pressed into the groove provided on the screw cover, rotates synchronously with the screw cover above the upper cover, and the injection hole is exposed when the screw cover is in the initial position, and the injection hole is sealed by the upper cover sealing element when the screw cover is rotated 90° clockwise from the initial position.

[0022] The upper cover sealing member and the screw cover sealing member are silica gel sealing members.

[0023] The disposable platelet-rich plasma separation device further comprises a lower cover, which is threadedly connected to the bottom end of the barrel body; a piston is fixed in the lower cover and is in interference fit with the barrel body.

[0024] Advantages:

[0025] (1) The disposable platelet-rich plasma separation device can directly complete the secondary centrifugation process without replacing the centrifugal tube, thereby simplifying the operation steps and improving the preparation efficiency.

[0026] (2) The device avoids the pollution caused by the contact with air when the PRP and the PPP layer are transferred to a new centrifugal tube in the traditional method, thereby reducing the risk of contamination of the plasma.

[0027] (3) The device ensures the effective concentration of PRP in the secondary centrifugation process through the precise through-hole design and the lifting tube structure, improves the platelet concentration of the final product, and meets the demand of high-concentration PRP in clinical practice.

[0028] (4) The device adopts the design that the screw cover drives the lifting tube to lift, and the clever cooperation of the upper cover and the screw cover makes the operation more convenient and fast, reduces the operation difficulty and human error.

[0029] (5) The device has a compact overall structure, excellent sealing performance, can effectively prevent blood leakage and external pollution, and ensures the safety and reliability of the preparation process. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 FIG. 1 is a structural schematic view of the disposable platelet-rich plasma separation device of the present application;

[0031] Figure 2 FIG. 4 is a schematic view of the connection relationship of the insertion rod, the lifting tube and the upper cover;

[0032] Figure 3 FIG. 5 is a schematic view of the layering of the blood sample in the disposable platelet-rich plasma separation device of the present application after the initial centrifugation;

[0033] Figure 4 FIG. 6 is a schematic view of the position of PRP in the disposable platelet-rich plasma separation device of the present application after the rotation of the lower cover to adjust the position of PRP;

[0034] Figure 5 FIG. 7 is a schematic view of the storage cavity in the barrel body, which is divided into upper and lower parts after the lower end of the lifting tube is closed to the upper end of the middle hollow cylinder.

[0035] Figure 6 Positional schematic view of the secondary centrifuged PRP in the disposable platelet-rich plasma separation device of the present application;

[0036] Figure 7 Process schematic view of extracting PRP by using a syringe;

[0037] Wherein, 1-rod, 2-rotary cap, 3-upper cover, 4-lifting tube, 5-through hole, 6-lower cover, 7-upper hollow cylinder, 8-upper hollow inverted round table, 9-intermediate hollow cylinder, 10-lower hollow round table, 11-lower hollow cylinder. DETAILED DESCRIPTION

[0038] The present application will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present application and not used to limit the scope of the present application. In addition, it should be understood that after reading the content taught by the present application, those skilled in the art can make various changes or modifications to the present application, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0039] A disposable platelet-rich plasma separation device, as shown in Figure 1 、 Figure 2 , comprises a barrel, a lifting tube 4, a rotary cap 2, a rod 1, an upper cover 3, a silica gel sealing member I, a silica gel sealing member II and a lower cover 6.

[0040] As shown in Figure 1 , the barrel is sequentially connected by the upper hollow cylinder 7, the upper hollow inverted round table 8, the intermediate hollow cylinder 9, the lower hollow round table 10 and the lower hollow cylinder 11 which are coaxial and have equal wall thickness; the large end of the upper hollow inverted round table 8 is upward and the small end is downward, the outer diameter of the large end is the same as that of the upper hollow cylinder 7, and the outer diameter of the small end is the same as that of the intermediate hollow cylinder 9; the small end of the lower hollow round table 10 is upward and the large end is downward, the outer diameter of the small end is the same as that of the intermediate hollow cylinder 9, and the outer diameter of the large end is the same as that of the lower hollow cylinder 11.

[0041] As shown in Figure 1 , the upper cover 3 is tightly screwed and locked with the top end of the barrel; the upper cover 3 is provided with an injection hole, and the silica gel sealing member I is used to close the injection hole; the upper cover 3 extends downward from the hole wall of the injection hole to form an upper cover 3 inner tube, and two symmetrical inclined grooves are arranged on the tube wall of the upper cover 3 inner tube, and the grooving range of the inclined grooves is 180°.

[0042] As shown in Figure 1As shown, the screw cap 2 is located above the upper cover 3, is rotatably connected to the upper cover 3, and only covers a partial area of ​​the upper cover 3; rotation refers to movement around a circular trajectory, and the center of the circular trajectory is located on the central axis of the upper cover 3; the installation position of the screw cap 2 and the silicone sealing member I meets the following requirements: the silicone sealing member I is completely pressed into the groove provided on the screw cap 2, and rotates synchronously with the screw cap 2 above the upper cover 3. When the screw cap 2 is in the initial position, the injection hole is exposed. When the screw cap 2 is rotated 90° clockwise from the initial position, the injection hole is blocked by the silicone sealing member I; an extraction hole is provided on the screw cap 2, and the silicone sealing member II is used to close the extraction hole; the screw cap 2 extends downward from the hole wall of the extraction hole to form an inner tube of the screw cap 2, and the inner tube of the screw cap 2 penetrates into the inner tube of the upper cover 3; two symmetrical vertical slots, two symmetrical upper horizontal slots and two symmetrical lower horizontal slots are provided on the tube wall of the inner tube of the screw cap 2, and the two vertical slots, the two upper horizontal slots and the two lower horizontal slots are connected to form two Z-shaped slots;

[0043] like Figure 2 As shown, the upper end of the lifting tube 4 penetrates into the inner tube of the screw cap 2, and the outer diameter of the lifting tube 4 is larger than the inner diameter of the central hollow cylinder 9. Two symmetrical protrusions are provided on the outer wall of the lifting tube 4. The two protrusions respectively pass through two vertical slots and then pass into two inclined grooves. The two inclined grooves are used to restrain the two protrusions of the lifting tube 4 from sliding up and down during rotation. The lower end of the lifting tube 4 is sealed, and a through hole 5 is provided on the tube wall near the lower end. The distance between the through hole 5 and the lower end of the lifting tube 4 is 2.5 to 5 mm. The number of through holes 5 is 2 or 4, and all through holes 5 are evenly distributed around the central axis of the lifting tube 4. The diameter of the through holes 5 is 0.5 to 3 mm.

[0044] like Figure 2 As shown, the insertion rod 1 is inserted into the lifting tube 4; when the insertion rod 1 moves downward to the limit, the through hole 5 is just closed by the lifting tube 4;

[0045] like Figure 1 As shown, the lower cover 6 is threadedly connected to the bottom end of the cylinder body; a piston is fixed in the lower cover 6, and the piston is interference fit with the cylinder body.

[0046] When using this device to separate PRP, the specific steps can be as follows:

[0047] (1) Initial centrifugation;

[0048] First, inject the blood sample to be processed into the barrel through the injection hole, then insert the rod into the bottom of the lifting tube, and then put the device into the centrifuge and perform a centrifugal treatment according to the predetermined centrifugal parameters. After the centrifugation is completed, Figure 3 As shown, the blood sample will be separated into three layers, from top to bottom: PPP, PRP and red blood cell layer;

[0049] (2) Adjust the PRP position;

[0050] After centrifugation, Figure 4 As shown, rotating the lower cover causes the liquid level to rise, guiding the initially separated PRP into the upper hollow rounded table;

[0051] (3) a liquid storage cavity inside the isolation barrel;

[0052] The rotating cover drives the lifting tube to move downward so that the lower end of the lifting tube closes the upper end of the middle hollow cylinder. At this time, Figure 5 As shown, the liquid storage cavity inside the barrel is divided into two parts by the lifting tube, the upper part is an upper hollow cylinder and an upper hollow inverted frustum, and the lower part is a middle hollow cylinder, a lower hollow frustum and a lower hollow cylinder;

[0053] (4) Secondary centrifugation;

[0054] The device is placed in the centrifuge again and centrifuged again according to the predetermined centrifugal parameters. Figure 6 As shown, after centrifugation, PRP is deposited at the bottom of the upper hollow inverted cone;

[0055] (5) Extraction of PRP;

[0056] like Figure 7 As shown, first pull out the insertion rod from the lifting tube, then pull the piston of the syringe to a position close to the needle of the syringe, then seal the needle of the syringe with the extraction hole, and then pull the piston of the syringe away from the needle of the syringe. During this process, the PRP at the bottom of the upper hollow rounded table will pass through the through hole and the lifting tube in turn and finally be sucked into the syringe (the volume of the syringe can be adjusted according to actual needs to accurately control the amount of PRP to be extracted. When the required amount is reached, stop pulling the piston and disconnect the syringe from the extraction hole).

Claims

1. A disposable platelet-rich plasma separation device, comprising a barrel, a lifting tube and a screw cap, wherein the lifting tube is inserted into the barrel, and the screw cap rotates to drive the lifting tube to move up and down, characterized in that: It also includes an insertion rod; the lower end of the lifting tube is sealed, and a through hole is provided on the tube wall near the lower end, and the diameter of the through hole is 0.5 to 3 mm; when the lifting tube moves downward to the limit, the liquid storage cavity inside the cylinder body is just divided into two parts, upper and lower parts, by the lifting tube; the insertion rod is inserted into the lifting tube, and when the insertion rod moves downward to the limit, the through hole is just closed by the lifting tube.

2. A disposable platelet-rich plasma separation device according to claim 1, characterized in that: The number of the through holes is 2 or 4, and they are evenly distributed around the central axis of the lifting tube.

3. A disposable platelet-rich plasma separation device according to claim 1, characterized in that: The distance between the through hole and the lower end of the lifting tube is 2.5 to 5 mm.

4. A disposable platelet-rich plasma separation device according to claim 1, characterized in that: The barrel is composed of an upper hollow cylinder, an upper hollow inverted cone, a middle hollow cylinder, a lower hollow cone and a lower hollow cylinder, which are coaxial and have equal wall thickness and are connected in sequence; the large end of the upper hollow inverted cone is at the top and the small end is at the bottom, the outer diameter of the large end is the same as the outer diameter of the upper hollow cylinder, and the outer diameter of the small end is the same as the outer diameter of the middle hollow cylinder; the small end of the lower hollow cone is at the top and the large end is at the bottom, the outer diameter of the small end is the same as the outer diameter of the middle hollow cylinder, and the outer diameter of the large end is the same as the outer diameter of the lower hollow cylinder; the outer diameter of the lifting tube is larger than the inner diameter of the middle hollow cylinder.

5. The disposable platelet-rich plasma separation device according to claim 1, characterized in that: It also includes an upper cover, an upper cover sealing member and a screw cap sealing member; The upper cover is screwed and locked to the top of the barrel; the screw cap is located above the upper cover, rotatably connected to the upper cover, and only covers a portion of the upper cover; rotation refers to movement around a circular trajectory, the center of the circular trajectory is located on the central axis of the upper cover; The upper cover is provided with an injection hole, the screw cap is provided with an extraction hole, the upper cover sealing piece is used to close the injection hole, and the screw cap sealing piece is used to close the extraction hole; The screw cap extends downward from the hole wall of the extraction hole to form a screw cap inner tube, and the upper cap extends downward from the hole wall of the injection hole to form an upper cap inner tube; There are two symmetrical protrusions on the outer wall of the lifting tube; there are two symmetrical vertical slots on the wall of the inner tube of the screw cap; there are two symmetrical inclined grooves on the wall of the inner tube of the upper cover, and the groove range of the inclined grooves is 180 degrees; The inner tube of the screw cap is inserted into the inner tube of the upper cap, the upper end of the lifting tube is inserted into the inner tube of the screw cap, and the two protrusions are respectively inserted into the two vertical slots and then into the two inclined grooves. The two inclined grooves are used to constrain the two protrusions of the lifting tube to slide up and down during the rotation process.

6. A disposable platelet-rich plasma separation device according to claim 5, characterized in that: Two symmetrical upper horizontal slots and two symmetrical lower horizontal slots are also provided on the tube wall of the screw cap inner tube. The two vertical slots, the two upper horizontal slots and the two lower horizontal slots are connected to form two Z-shaped slots.

7. The disposable platelet-rich plasma separation device according to claim 5, characterized in that: The installation position of the screw cap and the upper cover sealing member meets the following requirements: the upper cover sealing member is completely pressed into the groove provided on the screw cap, and rotates synchronously with the screw cap above the upper cover; when the screw cap is in the initial position, the injection hole is exposed; when the screw cap is rotated 90° clockwise from the initial position, the injection hole is blocked by the upper cover sealing member.

8. The disposable platelet-rich plasma separation device according to claim 5, characterized in that: The upper cover sealing piece and the screw cap sealing piece are silicone sealing pieces.

9. The disposable platelet-rich plasma separation device according to claim 1, characterized in that: The utility model also comprises a lower cover which is threadedly connected to the bottom end of the cylinder body; a piston is fixed in the lower cover, and the piston is interference-fitted with the cylinder body.

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