Electromagnetic magnetic frame for cell sorting

Abstract

The utility model provides an electromagnetic magnetic frame for cell sorting, which comprises a base, and a plurality of magnetic plates are arranged at the inner bottom of the base; a tube support for fixing the centrifugal tube is arranged on the outer side of the bottom of the base; the magnetic plate generates magnetic force to attract magnetic beads in the centrifugal tube. The magnetic plate is arranged at the bottom of the base, so that the magnetic distribution density of the bottom of the centrifugal tube is improved, the magnetic beads are more easily gathered, the adsorption quantity of the magnetic beads in the centrifugal tube is increased, and the magnetic bead loss generated in the magnetic separation process is reduced. And centrifugation is not needed in the sorting process, the cell sorting efficiency is improved, and the cell sorting time is shortened.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell sorting equipment technical field especially relates to a kind of electromagnetic magnetic force frame for cell sorting. BACKGROUND

[0002] In the cell engineering, protein engineering, biochemical engineering and other related experiments, magnetic bead method is a more common experimental method. The process of magnetic separation is basically: after sample and magnetic beads are combined, under the action of magnetic field, magnetic beads and sample coupling are adsorbed on the wall of centrifugal tube close to the magnet of magnetic force frame, at this time, the remaining solution in the centrifugal tube can be removed, and the separation of target and other substances is realized. In this process, the design and application of magnetic force frame have great influence on experimental results.

[0003] The traditional CTC cell sorting mostly uses magnetic beads that need to pass through the column. Although this method can separate relatively pure positive cells-magnetic beads and negative cells, the operation process is complex, and is mostly suitable for nanoscale small magnetic beads, with high requirements for magnetic bead technology. The existing magnetic force frame that can separate micron-sized magnetic beads is mostly suitable for small-volume centrifugal tubes, and the magnetic separation frame body that is suitable for 15 / 50mL centrifugal tube samples is relatively large, with uneven magnetic distribution, and magnetic beads are easy to disperse. SUMMARY

[0004] The utility model aims at providing a kind of electromagnetic magnetic force frame for cell sorting, can solve the problems of the existing magnetic separation frame body, uneven magnetic distribution and magnetic beads easy to disperse.

[0005] The utility model aims at realizing the following technical scheme:

[0006] A kind of electromagnetic magnetic force frame for cell sorting, including base, the inner bottom of base is provided with multiple magnetic plates;The bottom outer side of base is provided with tube holder for fixing centrifugal tube;Magnetic plate generates magnetic force and attracts the magnetic beads in centrifugal tube.

[0007] Preferably, the magnetic plate is an electromagnetic plate, which is composed of an iron core and a conductive coil wound around the iron core;The outer side of base is provided with power interface;The top of base is provided with multiple power switches, each power switch controls the on-off of one magnetic plate.

[0008] Preferably, the tube holder is provided with multiple hole positions, and the hole positions are in the shape of inverted pyramids;Each hole position is used for fixing a centrifugal tube, and each hole position corresponds to one magnetic plate.

[0009] Preferably, the electromagnetic magnetic force frame is suitable for 15mL centrifugal tube.

[0010] Preferably, the magnetic plate is a permanent magnet.

[0011] The utility model discloses a bottom of base is provided with the magnetic plate and improves the magnetic distribution density to the centrifugal tube bottom, makes the magnetic bead more easily gather, increases the adsorption amount of the magnetic bead in the centrifugal tube, thereby reduces the magnetic bead loss produced in the magnetic separation process. BRIEF DESCRIPTION OF DRAWINGS

[0012] Fig. 1 It is the internal structure schematic diagram of a kind of electromagnetic magnetic force frame for cell sorting of the utility model.

[0013] Fig. 2 It is the plan view of a kind of electromagnetic magnetic force frame for cell sorting of the utility model.

[0014] The reference signs are explained as follows:

[0015] 1: base, 2: magnetic plate, 3: tube holder, 4: power switch, 5: power interface, 6: hole site. DETAILED DESCRIPTION

[0016] The embodiments of the present disclosure are described in detail below with reference to the drawings.

[0017] The advantages and effects of the present disclosure can be easily understood by those skilled in the art from the content disclosed in the specification. Obviously, the described embodiments are only a part of the embodiments of the present disclosure, not all. The present disclosure can also be implemented or applied by other different specific embodiments, and the details in the specification can be modified or changed based on different views and applications without departing from the spirit of the present disclosure. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict. Based on the embodiments in the present disclosure, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present disclosure.

[0018] Embodiment one

[0019] The utility model discloses a bottom of base 1 is provided with the magnetic plate 2 and improves the magnetic distribution density to the centrifugal tube bottom, makes the magnetic bead more easily gather, increases the adsorption amount of the magnetic bead in the centrifugal tube, thereby reduces the magnetic bead loss produced in the magnetic separation process. Operation is simple, and the sorting process does not need to be centrifuged, improves cell sorting efficiency, and shortens cell sorting time.

[0020] As Figs. 1-2 shown, an electromagnetic magnetic force frame for cell sorting includes a base 1, the inner bottom of base 1 is provided with multiple magnetic plates 2, the bottom outer side of base 1 is provided with a tube holder 3 for fixing a centrifugal tube, and the magnetic plate 2 generates magnetic force to attract magnetic beads in the centrifugal tube.

[0021] Further, the magnetic plate 2 is an electromagnetic plate, which is composed of an iron core and a conductive coil wound on the iron core; the outer side of the base 1 is provided with a power supply interface 5; the top of the base 1 is provided with a plurality of power switches 4, each of which controls the on-off of one magnetic plate 2.

[0022] Further, the tube holder 3 is provided with a plurality of hole positions 6, which are inverted pyramids; each hole position 6 is used for fixing a centrifugal tube, and each hole position 6 corresponds to one magnetic plate 2.

[0023] Further, the electromagnetic magnetic force frame is adapted to a 15mL centrifugal tube.

[0024] Further, the magnetic plate 2 is a permanent magnet.

[0025] In the description of the present application, it should be understood that the orientation or positional relationship indicated by the terms "intermediate", "length", "upper", "lower", "front", "rear", "vertical", "horizontal", "inner", "outer", "radial", "circumferential" and the like is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application.

[0026] In the present application, unless otherwise explicitly specified and limited, the first feature "on" the second feature can be direct contact of the first and second features, or indirect contact of the first and second features through an intermediate medium. The meaning of "a plurality of" is at least two, for example, two, three, etc., unless otherwise explicitly specified and limited.

[0027] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected or in communication with each other; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship of two elements, unless otherwise explicitly limited. For ordinary skilled persons in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0028] The above is only to illustrate the embodiments of the present application, and is not used to limit the present application. For those skilled in the art, any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application without creative labor should be included in the protection scope of the present application.

Claims

1. An electromagnetic magnetic stand for cell sorting, characterized in that: The invention comprises a base (1), wherein a plurality of magnetic plates (2) are provided at the inner bottom of the base (1); a tube holder (3) for fixing a centrifuge tube is provided at the outer side of the bottom of the base (1); and the magnetic plates (2) generate magnetic force to attract the magnetic beads in the centrifuge tube.

2. The electromagnetic magnetic stand for cell sorting according to claim 1, wherein: The magnetic plate (2) is an electromagnetic plate, consisting of an iron core and a conductive coil wound around the iron core; a power interface (5) is provided on the outside of the base (1); and a plurality of power switches (4) are provided on the top of the base (1), each power switch (4) corresponding to the control of the power on and off of a magnetic plate (2).

3. The electromagnetic magnetic stand for cell sorting according to claim 1, wherein: The tube holder (3) is provided with a plurality of holes (6), each hole (6) being in the shape of an inverted cone; each hole (6) is used to fix a centrifuge tube, and each hole (6) corresponds to a magnetic plate (2).

4. The electromagnetic magnetic stand for cell sorting according to claim 1, wherein: The electromagnetic magnetic stand is suitable for 15 mL centrifuge tubes.

5. The electromagnetic magnetic stand for cell sorting according to claim 1, wherein: The magnetic plate (2) is a permanent magnet.

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