Mycobacterium tuberculosis culture device

Abstract

The utility model discloses a mycobacterium tuberculosis culture device, relates to bacterial culture field, including dish bottom and petri dish lid, petri dish lid threaded connection is dish bottom top, dish bottom and petri dish lid are both transparent glass material, dish bottom includes glass dish, rectangular groove and glass plate petri dish, rectangular groove is opened in glass dish bottom end, and the petri dish lid is opened in glass plate petri dish bottom end. A rectangular groove is formed in the bottom of the glass dish, a glass plate culture dish is inserted in the rectangular groove, a circular groove is formed in the inner wall of the bottom of the glass dish, and a threaded protrusion is fixedly connected to the inner wall of the top of the glass dish. According to the culture dish cover, a proper culture medium can be replaced to provide necessary nutrient substances such as a carbon source, a nitrogen source and inorganic salt required by growth of bacteria, so that the growth and reproduction requirements of the bacteria are met, and meanwhile, the convex lens is arranged in the culture dish cover, so that a user can conveniently carry out basic amplification observation on the cultured bacteria.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of bacterial culture, and specifically relates to a mycobacterium tuberculosis culture device. BACKGROUND

[0002] Mycobacterium tuberculosis is a pathogen of human tuberculosis, and is a long and slightly curved bacillus, has no flagellum, has bacterial hair, has a microcapsule but does not form a spore, has strong resistance to dry, cold, acid, alkali and other environments, and is a specific aerobic bacteria, that is, must grow and reproduce in an aerobic environment.

[0003] When mycobacterium tuberculosis is cultured, the current commonly used culture dish design has certain limitations. Most culture dishes are placed with a certain amount of nutrient medium in the inside to provide nutrients required for bacterial reproduction, however, mycobacterium tuberculosis consumes a large amount of nutrients in the reproduction process, especially in the long-term culture process, the consumption of nutrient medium is more significant.

[0004] In addition, the existing culture dish mostly adopts a sealing design of upper and lower cover bodies, this design can effectively prevent the entry of external pollutants, but at the same time, it also brings a problem, that is, it does not support replacing the nutrient medium in the bacterial reproduction process, which means that once the nutrient medium is consumed, the growth and reproduction of bacteria will be limited, thereby affecting the culture effect. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a mycobacterium tuberculosis culture device to solve the problems in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: a mycobacterium tuberculosis culture device, including dish bottom and culture dish cover, the culture dish cover is screw threaded in the dish bottom top end, the dish bottom and the culture dish cover are all transparent glass material, the dish bottom includes glass dish, rectangular groove and glass plate culture dish, the rectangular groove is opened in the glass dish bottom end, the glass plate culture dish is inserted in the rectangular groove inside.

[0007] Preferably, a circular groove is opened in the inner wall of the bottom of the glass dish, and a threaded convex is fixedly connected to the inner wall of the top of the glass dish.

[0008] Preferably, the culture dish cover includes a cover body, a threaded groove and a convex lens, a cover body is opened in the inner wall of the bottom of the cover body, an opening is opened in the top of the cover body, and the convex lens is fixedly connected in the opening.

[0009] Preferably, the threaded groove and the threaded convex are screw threaded to realize the installation of the culture dish cover on the top of the dish bottom.

[0010] Preferably, the circular groove and the rectangular groove are mutually through.

[0011] Preferably, the convex lens is located directly above the glass plate culture dish.

[0012] Compared with the prior art, the present application has the beneficial effects that:

[0013] The culture device for mycobacterium tuberculosis provided by the present application can replace the glass plate culture dish of the dish bottom according to the progress of the experiment, so as to optimize the bacterial growth environment, and replace the suitable culture medium to provide the necessary nutrients required for bacterial growth, such as carbon source, nitrogen source, inorganic salt, etc., so as to meet the needs of growth and reproduction, and the culture dish cover is provided with a convex lens, so that the user can perform basic magnified observation on the cultured bacteria. BRIEF DESCRIPTION OF DRAWINGS

[0014] The present application will be further described below in combination with the drawings:

[0015] Figure 1 The culture device for mycobacterium tuberculosis provided by the present application can replace the glass plate culture dish of the dish bottom according to the progress of the experiment, so as to optimize the bacterial growth environment, and replace the suitable culture medium to provide the necessary nutrients required for bacterial growth, such as carbon source, nitrogen source, inorganic salt, etc., so as to meet the needs of growth and reproduction, and the culture dish cover is provided with a convex lens, so that the user can perform basic magnified observation on the cultured bacteria. Figure One

[0016] Figure 2 The culture device for mycobacterium tuberculosis provided by the present application can replace the glass plate culture dish of the dish bottom according to the progress of the experiment, so as to optimize the bacterial growth environment, and replace the suitable culture medium to provide the necessary nutrients required for bacterial growth, such as carbon source, nitrogen source, inorganic salt, etc., so as to meet the needs of growth and reproduction, and the culture dish cover is provided with a convex lens, so that the user can perform basic magnified observation on the cultured bacteria. Figure Two

[0017] Figure 3 The culture device for mycobacterium tuberculosis provided by the present application can replace the glass plate culture dish of the dish bottom according to the progress of the experiment, so as to optimize the bacterial growth environment, and replace the suitable culture medium to provide the necessary nutrients required for bacterial growth, such as carbon source, nitrogen source, inorganic salt, etc., so as to meet the needs of growth and reproduction, and the culture dish cover is provided with a convex lens, so that the user can perform basic magnified observation on the cultured bacteria.

[0018] Figure 4 The culture device for mycobacterium tuberculosis provided by the present application can replace the glass plate culture dish of the dish bottom according to the progress of the experiment, so as to optimize the bacterial growth environment, and replace the suitable culture medium to provide the necessary nutrients required for bacterial growth, such as carbon source, nitrogen source, inorganic salt, etc., so as to meet the needs of growth and reproduction, and the culture dish cover is provided with a convex lens, so that the user can perform basic magnified observation on the cultured bacteria.

[0019] As shown in the drawings: 1, dish bottom; 11, glass dish; 12, rectangular groove; 13, glass plate culture dish; 14, circular groove; 15, threaded protrusion; 2, culture dish cover; 21, cover body; 22, threaded groove; 23, convex lens. DETAILED DESCRIPTION

[0020] In order to more clearly illustrate the overall concept of the present application, the following will be described in detail in an exemplary manner in combination with the drawings.

[0021] It should be noted that in the following description, many specific details are set forth in order to provide a thorough understanding of the present application, however, the present application can also be implemented in other ways different from those described herein, therefore, the protection scope of the present application is not limited by the specific embodiments disclosed below.

[0022] ​​In addition, in the description of the utility model, it is understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the utility model and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the utility model.

[0023] In the utility model, unless otherwise expressly specified and limited, the terms "mounting", "connection", "connecting", "fixing" and the like should be understood in a broad sense, for example, can be fixedly connected, or can be detachably connected, or can be integrated; can be directly connected, or can be indirectly connected through an intermediate medium, can be the communication inside two elements or the interaction relationship of two elements. However, it is noted that direct connection means that the connection between the two main bodies does not form a connection relationship through an excessive structure, but is connected to form a whole only through a transmission structure. For ordinary skilled persons in the art, the specific meanings of the above terms in the utility model can be understood according to the specific circumstances.

[0024] In the utility model, unless otherwise expressly specified and limited, the first feature is "on" or "under" the second feature, which can be direct contact of the first and second features, or indirect contact of the first and second features through an intermediate medium. In the description of the specification, the description of the reference terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In the specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0025] Please refer to Figures 1 to 4 The utility model provides a kind of technical scheme: a kind of mycobacterium tuberculosis culture device, including dish bottom 1 and culture dish cover 2, culture dish cover 2 is threadedly connected in dish bottom 1 top end, dish bottom 1 and culture dish cover 2 are transparent glass material, dish bottom 1 includes glass dish 11, rectangular groove 12 and glass plate culture dish 13, rectangular groove 12 is set in glass dish 11 bottom end, glass plate culture dish 13 is inserted in rectangular groove 12 inside;

[0026] In use, the glass plate culture dish 13 of the dish bottom 1 can be replaced as the experiment progresses to optimize the bacterial growth environment, and replacing the appropriate culture medium can provide the bacteria with the necessary nutrients required for growth, such as carbon source, nitrogen source, inorganic salt, etc., thereby meeting the needs of their growth and reproduction, while the convex lens 23 is built into the culture dish cover 2, which allows users to observe the cultured bacteria under a microscope.

[0027] The inner wall of the bottom of the glass dish 11 is provided with a circular groove 14, and the top inner wall of the glass dish 11 is fixedly connected with a threaded protrusion 15;

[0028] The culture dish cover 2 includes a cover body 21, a threaded groove 22, and a convex lens 23, the bottom inner wall of the cover body 21 is provided with a cover body 21, and the top end of the cover body 21 is provided with a through opening, and the convex lens 23 is fixedly connected in the opening;

[0029] The threaded groove 22 and the threaded protrusion 15 are threadedly connected to achieve the installation of the culture dish cover 2 on the top of the dish bottom 1;

[0030] The threaded installation method has strong airtightness and can reduce the invasion of external bacteria during bacterial culture;

[0031] The circular groove 14 and the rectangular groove 12 are mutually penetrated;

[0032] In use, the culture medium required for preparing bacteria is placed on the surface of the glass plate culture dish 13 in advance, the glass dish 11 is covered on the glass plate culture dish 13 through the rectangular groove 12, and the glass plate culture dish 13 is completely attached to the glass dish 11 to avoid the invasion of bacteria through the gap, after the bacteria reproduce in the glass dish 11, the nutrients in the culture medium are consumed to some extent, when the bacteria reproduction rate decreases or stops, the glass plate culture dish 13 can be removed by using the suction cup in the laboratory, and then another set of glass plate culture dish 13 loaded with new culture medium is replaced under the glass dish 11 to provide new nutrients for the bacteria, so that the bacteria can reproduce more effectively, thereby increasing the total number of bacteria;

[0033] The convex lens 23 is located directly above the glass plate culture dish 13;

[0034] The convex lens 23 can magnify the bacteria inside, and as the experiment progresses, the user can preliminarily observe the reproduction of the bacteria in the dish bottom 1 through the convex lens 23.

[0035] It should be understood by those of ordinary skill in the art that the above discussion of any of the embodiments is merely exemplary and is not intended to suggest that the scope of the application includes only these examples; it being further understood that the embodiments or the technical features among different embodiments can be combined, the steps can be implemented in any order, and there are many other changes of different aspects of the application as described above, which are not provided in details for the sake of brevity.

[0036] The present application is intended to cover all such alternatives, modifications, and variations as fall within the broad scope of the appended claims. Accordingly, any and all such alternatives, modifications and variations are intended to be encompassed by the appended claims.

Claims

1. A Mycobacterium tuberculosis culture device comprising a dish base (1) and a culture dish lid (2) characterised in that: Said culture dish cover (2) is screwed on the top of said dish bottom (1), said dish bottom (1) and said culture dish cover (2) are both transparent glass material, said dish bottom (1) comprises a glass dish (11), a rectangular groove (12) and a glass plate culture dish (13), said rectangular groove (12) is arranged at the bottom of said glass dish (11), and said glass plate culture dish (13) is inserted into said rectangular groove (12).

2. The device for culturing Mycobacterium tuberculosis according to claim 1, wherein: A circular groove (14) is arranged on the inner wall of the bottom of said glass dish (11), and a threaded convex (15) is fixedly connected to the inner wall of the top of said glass dish (11).

3. The M. tuberculosis culture device of claim 2, wherein: Said culture dish cover (2) comprises a cover body (21), a threaded groove (22) and a convex lens (23), a cover body (21) is arranged on the inner wall of the bottom of said cover body (21), an opening is arranged at the top of said cover body (21), and said convex lens (23) is fixedly connected in the opening.

4. The M. tuberculosis culture device of claim 3, wherein: Said culture dish cover (2) is installed on the top of said dish bottom (1) through the threaded connection between said threaded groove (22) and said threaded convex (15).

5. The M. tuberculosis culture device of claim 4, wherein: Said circular groove (14) and said rectangular groove (12) are mutually penetrated.

6. The M. tuberculosis culture device of claim 5, wherein: Said convex lens (23) is located directly above said glass plate culture dish (13).

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