Novel culture tray for determining pathogenicity of phytopathogen

Abstract

The utility model belongs to the technical field of biology, and discloses a novel culture tray for determining the pathogenicity of phytopathogen, which comprises a tray body, a cover, an opening, a grid and a plug. According to the novel culture tray for determining the pathogenicity of the phytopathogen, one tray can be used for determining the pathogenicity of four pathogens, and the size of a single lattice is convenient for the parts such as leaves and stalks of most plants, so that the experiment is convenient to carry out. The length, width and height of the whole tray are matched with those of most incubators, so that the space of the incubators can be fully utilized, and the operation is convenient. The single cell is provided with an independent hole with a plug, so that water can be conveniently added at any time in the experiment process, the frequency and the area of contact with air are reduced, and the problem of pollution easily occurring in the experiment process is greatly relieved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the field of biotechnology, especially relates to a novel culture plate for pathogenicity determination of plant pathogenic bacteria. BACKGROUND

[0002] In the process of identifying plant pathogens, pathogenicity determination experiments must be carried out, and many pathogenicity determination experiments are carried out using in vitro plant tissues. Since each experiment requires three biological repeats, the culture dishes currently used are circular and have few size types, which is not convenient for pathogenicity determination experiments of in vitro plant tissues.

[0003] Through the above analysis, the problems and defects of the prior art are:

[0004] Since each experiment requires three biological repeats, the culture dishes currently used are circular and have few size types, which is not convenient for pathogenicity determination experiments of in vitro plant tissues. SUMMARY

[0005] In view of the problems in the prior art, the utility model provides a novel culture plate for pathogenicity determination of plant pathogenic bacteria.

[0006] The utility model is realized in this way, a novel culture plate for pathogenicity determination of plant pathogenic bacteria includes:

[0007] The disc body, the lid, the hole, the square, the plug;

[0008] The disc body is provided with a square; the top surface of the square is provided with a lid; the edge of the square is provided with a hole; the hole is provided with a plug.

[0009] Further, the square is 7cm wide and 10cm long.

[0010] Further, the disc body is 3cm high.

[0011] In combination with the above technical solutions and solved technical problems, the technical solution to be protected by the utility model has the following advantages and positive effects:

[0012] The structure device design of the utility model realizes the significant technical effect of pathogenicity determination of plant pathogenic bacteria, which is embodied in the following aspects:

[0013] 1) Independent square design: each square forms an independent culture space in the disc body, ensuring that the experimental environments of each strain are independent of each other, avoiding the risk of cross contamination. This design improves the reliability of the experiment, making it possible to culture and determine multiple strains at the same time, thereby greatly improving the experimental efficiency.

[0014] 2) Independent cover on top of each square: The independent cover effectively seals each square, maintaining constant humidity and temperature inside, ensuring the stability of the culture conditions. The cover design makes it easier to control and monitor the environment of each square, helping various strains to be cultured under precise conditions, improving the accuracy of experimental results.

[0015] 3) Square opening and sealing plug: The hole on the side of the square and the plug allow the experimenter to adjust the ventilation inside the square flexibly to meet the oxygen needs of different strains. This design not only simplifies operations such as nutrient solution addition, but also ensures that the possibility of external contamination is reduced during reagent addition, thereby improving the accuracy and convenience of the experiment.

[0016] 4) Appropriate height of the tray: The 3cm height of the tray provides sufficient space for bacterial growth and medium containment, suitable for pathogenicity testing of various strains. This height also prevents the overflow of culture solution, ensuring the cleanliness of the experiment and providing more space for experimental operations, improving the convenience and safety of operations.

[0017] 5) Modularity and versatility: Each square, cover, opening, plug, and other designs work together to form a modular combination structure, allowing the culture plate to adapt to different experimental needs. This modular design not only facilitates maintenance, but also provides the possibility for further expansion and optimization, such as replacing the cover or adjusting the plug configuration in certain experiments to achieve personalized experimental conditions.

[0018] The utility model discloses a novel plant pathogenic bacteria pathogenicity determination culture plate, and four kinds of pathogenicity determination of pathogenic bacteria can be carried out in a tray. The size of the single square is convenient for the leaves, stems and other parts of most plants, and facilitates the experiment. The size of the whole tray length, width and height is compatible with the size of most incubators, which can fully utilize the space of the incubator and is convenient to operate. The single square is provided with an independent plug hole, which is convenient for adding water at any time during the experiment, reduces the frequency and area of air contact, and greatly reduces the pollution problem that is prone to occur during the experiment.

[0019] The technical scheme of the utility model after transformation has the expected income and commercial value: after the transformation of the technical scheme of the utility model, the time cost, space cost and labor cost required for plant pathogenic bacteria pathogenicity determination test can be greatly saved, so the utility model has certain commercial value.

[0020] The technical scheme of the utility model fills the technical blank in the industry at home and abroad: the technical scheme of the utility model fills the technical blank in the industry at home and abroad, especially provides great convenience for large quantities of plant pathogenic bacteria pathogenicity determination test.

[0021] The technical scheme of the utility model overcomes technical prejudice: in the process of plant pathogenic bacteria pathogenicity determination test, there may be mixed bacteria pollution, the technical scheme of the utility model after transformation can reduce the probability of mixed bacteria pollution, and can make up for the previous technical loophole.

[0022] The optimized design of these structural devices makes the culture plate of the utility model have significant improvement in experimental precision, convenience and diversity, not only meets the core demand of plant pathogenic bacteria pathogenicity determination, but also provides guarantee for standardization and high efficiency of experiment. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 It is the structure diagram of the novel culture plate for plant pathogenic bacteria pathogenicity determination provided by the utility model embodiment.

[0024] Figure 2 It is the side view of the novel culture plate for plant pathogenic bacteria pathogenicity determination provided by the utility model embodiment.

[0025] Figure 3 It is the structure diagram of the opening provided by the utility model embodiment.

[0026] Figure 1 In the middle: 1, disc body;2, cover;3, opening;4, square;5, plug. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the utility model more clear and obvious, the utility model is further described in detail in combination with embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model, and are not used to limit the utility model.

[0028] The culture plate for plant pathogenic bacteria pathogenicity determination of the utility model is designed ingeniously, through reasonable arrangement on structure, the efficient support to pathogenic bacteria pathogenicity experiment is realized. First, the culture plate main body is composed of disc body and cover, a plurality of square is distributed in the disc body, each square forms independent culture space, which is convenient for partition management of different strains or samples in the experimental process, and avoids cross contamination. At the same time, the square design size is 7cm wide, 10cm long, which can provide sufficient growth and expansion space for pathogenic bacteria, so that the strain can fully display pathogenic characteristics in the culture process, and ensure the accuracy and reliability of the result.

[0029] Secondly, each square top is equipped with independent cover, the cover not only can seal the square, maintain the internal suitable humidity and temperature environment, but also can effectively isolate the interference factors of external environment. The design of independent cover forms independent culture environment between different squares, so that the experimental personnel can better observe the independent growth of each strain under consistent conditions when researching multiple strains, and effectively improve the scientificity of experiment.

[0030] Third, the side of the grid is designed with openings, which provide convenience for experimental operation. Through the openings, experimenters can add necessary reagents such as nutrient solution and culture medium to the inside of the grid without opening the lid, which greatly reduces the risk of external contamination during the experiment. In addition, the openings also facilitate ventilation adjustment during the experiment, ensuring that the strain can obtain the required oxygen and carbon dioxide to maintain good growth conditions.

[0031] Fourth, a dedicated plug is provided in each opening. The plug can be inserted or removed at any time according to experimental needs, controlling the flow rate of gas and achieving microenvironment adjustment. This design is particularly suitable for experiments sensitive to strain growth environment, and experimenters can control the amount of ventilation by adjusting the sealing state of the plug to adapt to the growth requirements of different plant pathogens, providing more precise experimental conditions.

[0032] Fifth, the overall side height of the disc body is 3 cm, providing sufficient space height for the culture medium and bacterial body inside the grid. Such design can meet the growth needs of various strains, and also provides experimenters with more operating space for adding reagents or observation during the experiment. In addition, the side height of the disc body can effectively prevent the culture solution from overflowing, maintaining the cleanliness of the experimental environment.

[0033] Finally, the structure design of the culture plate not only considers the functional needs of the experiment, but also focuses on the simplicity and safety of experimental operation. Through the independent grid, lid and opening structure, the culture plate can meet the needs of different plant pathogen pathogenicity determination and reduce the risk of external contamination. This design integrates precise microenvironment control, convenient operation and efficient isolation protection, providing an integrated and standardized experimental tool for plant pathogen pathogenicity determination, which helps to improve the reliability of the experiment and the efficiency of the operation.

[0034] As shown in Figure 1 , 2 , the utility model embodiment provides a novel culture plate for plant pathogen pathogenicity determination, which comprises:

[0035] Disc body 1, lid 2, opening 3, grid 4, plug 5;

[0036] The disc body 1 is provided with the grid 4; the top surface of the grid 4 is provided with the lid 2; the edge of the grid 4 is provided with the opening 3;

[0037] As shown in Figure 3 , the opening 3 is provided with the plug 5.

[0038] The grid 4 provided by the utility model embodiment is 7 cm wide and 10 cm long.

[0039] The disc body 1 is 3cm high.

[0040] The following are two specific embodiments based on the above description:

[0041] Embodiment 1

[0042] The embodiment provides a novel culture plate suitable for pathogenicity determination of various plant pathogens.

[0043] 1. Culture plate structure: the disc body 1 is a rectangular structure, and the side height is 3cm. The disc body is uniformly provided with 20 independent squares 4, and the width of the square is 7cm and the length is 10cm. Each square bottom is provided with a microporous ventilation design to ensure the gas exchange inside the culture medium.

[0044] 2. Square hole and sealing: the edge of the square 4 is provided with a circular hole 3 with a diameter of 1cm, which is used for the addition of external gas or reagent. The hole is inserted with a detachable silica gel plug 5, which is convenient for repeated use and also plays a sealing role to prevent external pollution.

[0045] 3. Lid design: the lid 2 covers the top of the square 4, and the lid material is transparent polypropylene, which prevents excessive evaporation of water during the culture process and provides light transmittance, facilitating observation of the growth state of the internal pathogen.

[0046] 4. Applicability: the culture plate is particularly suitable for pathogenicity testing of small-scale various plant pathogens, and is convenient for observing and recording the differences between different treatment groups.

[0047] Embodiment 2

[0048] The embodiment shows a multi-grid culture plate convenient for controlling humidity and ventilation.

[0049] 1. Culture plate specification: the disc body 1 is made of high-temperature-resistant polystyrene material, and the side height is 3cm, and the inside is provided with 16 squares 4. Each square has a size of 7cm in width and 10cm in length, providing sufficient space for pathogen culture and growth observation.

[0050] 2. Hole and plug design: the edge of the square 4 is provided with a hole 3 with a diameter of 1.5cm, which is suitable for the injection requirement of a larger volume of reagent. The hole 3 is inserted with a washable rubber plug 5, and the rubber material has good sealing property and is resistant to chemical reagent corrosion, ensuring the safety of the experimental process.

[0051] 3. Lid and humidity adjustment: the lid 2 is made of transparent polycarbonate material, which has good air permeability and is suitable for culture experiments requiring high humidity environment. The edge of the lid is provided with a small air exhaust hole, which can increase or decrease the air flow according to the experimental requirements, facilitating the control of experimental humidity.

[0052] 4. Application field: the culture plate is suitable for resistance test of plant pathogenic bacteria, different effects of different pesticides can be compared through multiple squares, and the growth environment of the pathogenic bacteria is conveniently and accurately controlled.

[0053] The utility model is applied in the field of agriculture and is most suitable for plant protection.

[0054] In the process of plant pathogenic bacteria pathogenicity determination experiment, the utility model embodiment can simultaneously carry out repeated test of multiple pathogenic bacteria, and greatly reduces the time consumption. For inoculation pathogenic bacteria experiment of different plant tissues, the invention can meet the experimental requirements of different plant parts, and solves the problem that ordinary culture dishes cannot meet some plant parts, thereby leading to inaccurate pathogenicity determination experiment results.

[0055] The above is only a specific embodiment of the utility model, but the protection scope of the utility model is not limited to this, any skilled person in the art can make any modification, equivalent replacement and improvement within the technical range disclosed by the utility model, as long as it is within the spirit and principle of the utility model, which should be covered in the protection scope of the utility model.

Claims

1. A novel culture plate for plant pathogenicity assay of a new plant pathogenic fungus, characterized in that, It includes: Disc body, cover, opening, square, plug; The disc body is internally provided with a square; The top surface of the square is provided with a cover; the edge of the square is provided with an opening; The opening is internally provided with a plug.

2. The new type of plant pathogenic bacteria pathogenicity assay plate according to claim 1, wherein, The square is 7 cm wide and 10 cm long.

3. The new type of plant pathogenic bacteria pathogenicity assay plate according to claim 1, wherein, The disc body is 3 cm high on the side.

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