Wide fluorescence quantitative detection device and sample concentration detection equipment

Abstract

The utility model discloses a wide fluorescence quantitative detection device and sample concentration detection equipment, and the wide fluorescence quantitative detection device comprises a photoelectric conversion circuit which is used for receiving a fluorescence signal of a sample and converting the fluorescence signal into a voltage signal; the signal amplification circuit is connected with the photoelectric conversion circuit and used for amplifying the voltage signals to obtain n voltage amplification signals, the amplification times of the voltage amplification signals are different, and n is a positive integer larger than or equal to 2; the voltage acquisition circuit is connected with the signal amplification circuit and is used for sampling the n voltage amplification signals according to a preset acquisition interval to obtain n groups of voltage data; and the controller is connected with the voltage acquisition circuit and is used for obtaining a concentration value corresponding to the sample according to the n groups of voltage data. According to the invention, a large concentration detection range can be covered, and an accurate detection result can be obtained when the actual concentration value of a sample is small or large.

Description

Technical Field

[0001] This application relates to the field of broad fluorescence quantitative technology, specifically to a broad fluorescence quantitative detection device and a sample concentration detection device. Background Technology

[0002] Currently, quantitative fluorescence detection is an analytical method widely used in fields such as biomedicine, environmental science, and food safety, and can be used to quantitatively determine the concentration of various substances.

[0003] Current quantitative fluorescence detection technologies use photoelectric technology to detect the concentration of reagent molecules. This involves irradiating the fluorescent groups bound to the molecular fragments with excitation light and then receiving the fluorescence signal returned by the fluorescent groups. The concentration of the sample is then determined based on the magnitude of the fluorescence signal. However, current quantitative fluorescence detection technologies cover a relatively small concentration range, and the detection results are inaccurate when dealing with samples with low or high concentrations. Utility Model Content

[0004] Therefore, this application provides a wide-range fluorescence quantitative detection device and a sample concentration detection equipment to cover a large concentration detection range, and to obtain accurate detection results even when the actual concentration value of the sample is small or large. The technical solution of this application is as follows:

[0005] The first aspect of this application provides a wide-range fluorescence quantitative detection device, comprising: a photoelectric conversion circuit for receiving a fluorescence signal from a sample and converting the fluorescence signal into a voltage signal; a signal amplification circuit connected to the photoelectric conversion circuit for amplifying the voltage signal to obtain n amplified voltage signals, wherein each amplified voltage signal has a different amplification factor, and n is a positive integer greater than or equal to 2; a voltage acquisition circuit connected to the signal amplification circuit for sampling the n amplified voltage signals at a preset acquisition interval to obtain n sets of voltage data; and a controller connected to the voltage acquisition circuit for obtaining the concentration value corresponding to the sample based on the n sets of voltage data.

[0006] In one embodiment of this application, the photoelectric conversion circuit includes a photoelectric sensor and a voltage conversion circuit; the photoelectric sensor is used to receive the fluorescence signal and convert the fluorescence signal into a current signal; the voltage conversion circuit is used to receive the current signal and convert the current signal into a voltage signal.

[0007] In one embodiment of this application, the voltage conversion circuit includes a first amplifier, a first resistor, a first capacitor, a second capacitor, a third capacitor, and a fourth capacitor; the positive input terminal of the first amplifier is connected to the positive terminal of the photoelectric sensor, and the negative input terminal of the first amplifier is connected to the negative terminal of the photoelectric sensor; the first terminal of the first resistor is connected to the negative input terminal of the first amplifier, and the second terminal of the first resistor is connected to the output terminal of the first amplifier; the first capacitor is connected in parallel with the first resistor; the power supply terminal of the first amplifier is used to receive the power supply voltage and is grounded through the second capacitor; the first terminal of the third capacitor is connected to the positive terminal of the photoelectric sensor and receives a preset voltage, and the second terminal of the third capacitor is grounded; the fourth capacitor is connected in parallel with the third capacitor.

[0008] In one embodiment of this application, the signal amplification circuit includes a first voltage follower circuit, a second voltage follower circuit, a first amplification circuit, and a second amplification circuit; the first voltage follower circuit is connected to the photoelectric conversion circuit and is used to receive the voltage signal and output it to the voltage acquisition circuit; the first amplification circuit is connected to the photoelectric conversion circuit and is used to amplify the voltage signal by a first preset factor to obtain a first voltage amplification signal; the second voltage follower circuit is connected to the first amplification circuit and is used to receive the first voltage amplification signal and output it to the voltage acquisition circuit; the second amplification circuit is connected to the first amplification circuit and is used to amplify the first voltage amplification signal by a second preset factor to obtain a second voltage amplification signal and output it to the voltage acquisition circuit.

[0009] In one embodiment of this application, the first voltage follower circuit includes a second amplifier, a second resistor, and a fifth capacitor; the positive input terminal of the second amplifier is connected to the photoelectric conversion circuit through the second resistor, the negative input terminal of the second amplifier is connected to the output terminal of the second amplifier, and the output terminal of the second amplifier is connected to the voltage acquisition circuit; the first terminal of the fifth capacitor is connected to the positive input terminal of the second amplifier, and the second terminal of the fifth capacitor is grounded.

[0010] In one embodiment of this application, the first amplification circuit includes a third amplifier, a third resistor, a fourth resistor, and a sixth capacitor; the positive input terminal of the third amplifier is connected to the photoelectric conversion circuit; the negative input terminal of the third amplifier is grounded through the third resistor and connected to the output terminal of the third amplifier through the fourth resistor; the power supply terminal of the third amplifier is used to receive the power supply voltage and is grounded through the sixth capacitor.

[0011] In one embodiment of this application, the second follower circuit includes a fourth amplifier, a fifth resistor, and a seventh capacitor; the positive input terminal of the fourth amplifier is connected to the first amplifier circuit through the fifth resistor, the negative input terminal of the fourth amplifier is connected to the output terminal of the fourth amplifier, and the output terminal of the fourth amplifier is connected to the voltage acquisition circuit; the first terminal of the seventh capacitor is connected to the positive input terminal of the fourth amplifier, and the second terminal of the seventh capacitor is grounded.

[0012] In one embodiment of this application, the second amplification circuit includes a fifth amplifier, a sixth resistor, a seventh resistor, and an eighth capacitor; the positive input terminal of the fifth amplifier is connected to the first amplification circuit; the negative input terminal of the fifth amplifier is grounded through the sixth resistor and connected to the output terminal of the fifth amplifier through the seventh resistor; the power supply terminal of the fifth amplifier is used to receive the power supply voltage and is grounded through the eighth capacitor.

[0013] In one embodiment of this application, the controller is further configured to obtain the concentration value corresponding to the sample based on n sets of voltage data and a preset regression equation.

[0014] A second aspect of this application provides a sample concentration detection device, including the aforementioned wide fluorescence quantitative detection device.

[0015] The wide fluorescence quantitative detection device of this application converts the fluorescence signal of the sample into a voltage signal through a photoelectric conversion circuit. Then, the voltage signal is amplified by a signal amplification circuit to obtain n voltage amplification signals with different amplification factors, thereby obtaining a wide voltage amplification signal range. The voltage acquisition circuit then acquires the wide range of voltage amplification signals to obtain a wide range of voltage data. Finally, the controller obtains the concentration value of the sample based on the voltage data. Because a wide voltage amplification signal range is obtained, accurate detection results can be obtained even when the actual concentration value of the sample is small or large. Attached Figure Description

[0016] Figure 1 This is a schematic block diagram of a wide fluorescence quantitative detection device provided in an embodiment of this application.

[0017] Figure 2 This is a schematic block diagram of a photoelectric conversion circuit provided in an embodiment of this application.

[0018] Figure 3 This is a circuit diagram of a photoelectric conversion circuit provided in an embodiment of this application.

[0019] Figure 4 This is a schematic block diagram of a signal amplification circuit provided in an embodiment of this application.

[0020] Figure 5 This is a circuit diagram of a signal amplification circuit provided in an embodiment of this application. Detailed Implementation

[0021] It should be noted that in the embodiments of this application, "at least one" refers to one or more, and "more than one" refers to two or more. "And / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone, where A and B can be singular or plural. The terms "first," "second," "third," "fourth," etc. (if present) in the specification, claims, and drawings of this application are used to distinguish similar objects, not to describe a specific order or sequence.

[0022] It should also be noted that the methods disclosed in the embodiments of this application or the methods shown in the flowcharts include one or more steps for implementing the method. Without departing from the scope of the claims, the execution order of multiple steps can be interchanged, and some steps can also be deleted.

[0023] Quantitative fluorescence detection is an analytical method widely used in fields such as biomedicine, environmental science, and food safety. It can be used to quantitatively determine the concentration of various substances.

[0024] Current quantitative fluorescence detection technologies use photoelectric technology to detect the concentration of reagent molecules. This involves irradiating the fluorescent groups bound to the molecular fragments with excitation light and then receiving the fluorescence signal returned by the fluorescent groups. The concentration of the sample is then determined based on the magnitude of the fluorescence signal. However, current quantitative fluorescence detection technologies cover a relatively small concentration range, and the detection results are inaccurate when dealing with samples with low or high concentrations.

[0025] This application provides a wide fluorescence quantitative detection device and a sample concentration detection device to cover a large concentration detection range, and to obtain accurate detection results even when the actual concentration value of the sample is small or large.

[0026] Please refer to Figure 1 , Figure 1 This is a schematic block diagram of a wide fluorescence quantitative detection device provided in an embodiment of this application. The wide fluorescence quantitative detection device 100 includes a photoelectric conversion circuit 110, a signal amplification circuit 120, a voltage acquisition circuit 130, and a controller 140.

[0027] In this embodiment, the photoelectric conversion circuit 110 receives the fluorescence signal of the sample and converts it into a voltage signal. The signal amplification circuit 120, connected to the photoelectric conversion circuit 110, amplifies the voltage signal to obtain n amplified voltage signals, where each signal has a different amplification factor, and n is a positive integer greater than or equal to 2. The voltage acquisition circuit 130, connected to the signal amplification circuit 120, samples the n amplified voltage signals at preset acquisition intervals to obtain n sets of voltage data. The controller 140, connected to the voltage acquisition circuit 130, obtains the concentration value corresponding to the sample based on the n sets of voltage data.

[0028] It is understood that the wide fluorescence quantitative detection device of this application converts the fluorescence signal of the sample into a voltage signal through a photoelectric conversion circuit, and then amplifies the voltage signal through a signal amplification circuit to obtain n voltage amplification signals with different amplification factors, thereby obtaining a wide voltage amplification signal range. Then, the voltage acquisition circuit acquires the wide range of voltage amplification signals to obtain a wide range of voltage data. Finally, the controller obtains the concentration value of the sample based on the voltage data. Because a wide voltage amplification signal range is obtained, accurate detection results can be obtained even when the actual concentration value of the sample is small or large.

[0029] Please refer to Figure 2 In some embodiments, the photoelectric conversion circuit 110 includes a photoelectric sensor 111 and a voltage conversion circuit 112.

[0030] The photoelectric sensor 111 is used to receive fluorescence signals and convert them into current signals. The voltage conversion circuit 112 is used to receive current signals and convert them into voltage signals.

[0031] Please refer to Figure 3 , Figure 3 This is a circuit diagram of a photoelectric conversion circuit 110 provided in an embodiment of this application. The voltage conversion circuit 112 includes a first amplifier U1, a first resistor R1, a first capacitor C1, a second capacitor C2, a third capacitor C3, and a fourth capacitor C4.

[0032] In this embodiment, the positive input terminal of the first amplifier U1 is connected to the positive terminal of the photoelectric sensor 111, and the negative input terminal of the first amplifier U1 is connected to the negative terminal of the photoelectric sensor 111. The first terminal of the first resistor R1 is connected to the negative input terminal of the first amplifier U1, and the second terminal of the first resistor R1 is connected to the output terminal of the first amplifier U1. The first capacitor C1 is connected in parallel with the first resistor R1. The power supply terminal of the first amplifier U1 receives the power supply voltage V and is grounded through the second capacitor C2. The first terminal of the third capacitor C3 is connected to the positive terminal of the photoelectric sensor 111 and receives a preset voltage Vre; the second terminal of the third capacitor C3 is grounded. The fourth capacitor C4 is connected in parallel with the third capacitor C3.

[0033] It is understood that by inputting a fixed preset voltage Vre to the non-inverting terminal of the voltage conversion circuit 112, and then grounding it after passing through the second capacitor C2, the noise effect of grounding can be effectively eliminated. Simultaneously, the positive input terminal of the first amplifier U1 is connected to the positive terminal of the photoelectric sensor 111, and the negative input terminal of the first amplifier U1 is connected to the negative terminal of the photoelectric sensor 111. The current signal from the photoelectric sensor 111 can be converted into the aforementioned voltage signal through the first resistor R1.

[0034] Please refer to Figure 4 In some embodiments, the signal amplification circuit 120 includes a first voltage follower circuit 121, a second voltage follower circuit 122, a first amplification circuit 123, and a second amplification circuit 124.

[0035] The circuit comprises: a first voltage follower circuit 121 connected to a photoelectric conversion circuit 110, used to receive voltage signals and output them to a voltage acquisition circuit 130; a first amplifier circuit 123 connected to a photoelectric conversion circuit 110, used to amplify the voltage signal by a first preset factor to obtain a first voltage amplified signal; a second voltage follower circuit 122 connected to a first amplifier circuit 123, used to receive the first voltage amplified signal and output it to the voltage acquisition circuit 130; and a second amplifier circuit 124 connected to a first amplifier circuit 123, used to amplify the first voltage amplified signal by a second preset factor to obtain a second voltage amplified signal, which is then output to the voltage acquisition circuit 130.

[0036] Please refer to Figure 5 , Figure 5 This is a circuit diagram of a signal amplification circuit 120 provided in an embodiment of this application.

[0037] The first voltage follower circuit 121 includes a second amplifier U2, a second resistor R2, and a fifth capacitor C5. The positive input terminal of the second amplifier U2 is connected to the photoelectric conversion circuit 110 through the second resistor R2, the negative input terminal of the second amplifier U2 is connected to the output terminal of the second amplifier U2, and the output terminal of the second amplifier U2 is connected to the voltage acquisition circuit 130. The first terminal of the fifth capacitor C5 is connected to the positive input terminal of the second amplifier U2, and the second terminal of the fifth capacitor C5 is grounded.

[0038] The first amplifier circuit 123 includes a third amplifier U3, a third resistor R3, a fourth resistor R4, and a sixth capacitor C6. The positive input terminal of the third amplifier U3 is connected to the photoelectric conversion circuit 110. The negative input terminal of the third amplifier U3 is grounded through the third resistor R3 and connected to the output terminal of the third amplifier U3 through the fourth resistor R4. The power supply terminal of the third amplifier U3 is used to receive the power supply voltage V and is grounded through the sixth capacitor C6.

[0039] The second follower circuit includes a fourth amplifier U4, a fifth resistor R5, and a seventh capacitor C7. The positive input terminal of the fourth amplifier U4 is connected to the first amplifier circuit 123 through the fifth resistor R5, and the negative input terminal of the fourth amplifier U4 is connected to the output terminal of the fourth amplifier U4. The output terminal of the fourth amplifier U4 is connected to the voltage acquisition circuit 130. The first terminal of the seventh capacitor C7 is connected to the positive input terminal of the fourth amplifier U4, and the second terminal of the seventh capacitor C7 is grounded.

[0040] The second amplifier circuit 124 includes a fifth amplifier U5, a sixth resistor R6, a seventh resistor R7, and an eighth capacitor C8. The positive input terminal of the fifth amplifier U5 is connected to the first amplifier circuit 123. The negative input terminal of the fifth amplifier U5 is grounded through the sixth resistor R6 and connected to the output terminal of the fifth amplifier U5 through the seventh resistor R7. The power supply terminal of the fifth amplifier U5 is used to receive the power supply voltage V and is grounded through the eighth capacitor C8.

[0041] In some embodiments, the fourth resistor R4 and the seventh resistor R7 can be adjusted according to the desired amplification factor to obtain the desired amplification factor.

[0042] In some embodiments, the controller 140 is further configured to obtain the concentration value corresponding to the sample based on n sets of voltage data and a preset regression equation.

[0043] The controller can store n sets of preset regression equations in advance. Each set of preset regression equations corresponds to a set of voltage data for a certain amplification factor. The concentration values ​​of n samples are calculated and then the arithmetic average is performed to obtain the final sample concentration value.

[0044] In some embodiments, after obtaining n sets of voltage data, the controller can also remove some data from the voltage data to improve calculation efficiency and accuracy. For example, if a set of voltage data contains 100 voltage values, the first 20 voltage values ​​and the last 20 voltage values ​​can be removed, and 60 voltage values ​​can be retained to calculate the sample concentration value corresponding to the set of voltage data.

[0045] This application also provides a sample concentration detection device, including the broad fluorescence quantitative detection device of any of the above embodiments. It is understood that the beneficial effects of the sample concentration detection device can be referred to the beneficial effects of the broad fluorescence quantitative detection device in the foregoing embodiments, and will not be repeated here.

[0046] In some embodiments, when using the above-described sample concentration detection device to detect the concentration of the sample, the following steps may also be included:

[0047] (1) Initialize the sample concentration detection equipment;

[0048] (2) Using a sample concentration detection device, multiple nth standard samples with known concentrations of Xn are detected to obtain multiple sets of voltage data Yn;

[0049] For example, a sample concentration detection device can be used to detect a first standard with a known concentration of X1 to obtain voltage data Y1, and the same device can be used to detect a second standard with a known concentration of X2 to obtain voltage data Y2.

[0050] (3) Based on multiple known concentrations Xn and corresponding sets of voltage data Yn, construct and update multiple sets of regression equations;

[0051] (4) Use the sample concentration detection device to detect the sample and obtain multiple sets of voltage data. Substitute the voltage data into the above multiple sets of regression equations to obtain the concentration value of the sample.

[0052] The above embodiments are merely preferred embodiments of this application and are not intended to limit the scope of this application. Any modifications and improvements made by those skilled in the art to the technical solutions of this application without departing from the spirit of this application should fall within the protection scope defined by the claims of this application.

Claims

1. A wide-range fluorescence quantitative detection device, characterized in that, include: A photoelectric conversion circuit is used to receive the fluorescence signal of the sample and convert the fluorescence signal into a voltage signal; A signal amplification circuit, connected to the photoelectric conversion circuit, is used to amplify the voltage signal to obtain n voltage amplification signals, wherein the amplification factor of each voltage amplification signal is different, and n is a positive integer greater than or equal to 2; A voltage acquisition circuit, connected to the signal amplification circuit, is used to sample n voltage amplification signals at a preset acquisition interval to obtain n sets of voltage data. The controller, connected to the voltage acquisition circuit, is used to obtain the concentration value corresponding to the sample based on n sets of voltage data.

2. The wide-range fluorescence quantitative detection device as described in claim 1, characterized in that, The photoelectric conversion circuit includes a photoelectric sensor and a voltage conversion circuit; The photoelectric sensor is used to receive the fluorescence signal and convert the fluorescence signal into a current signal; The voltage conversion circuit is used to receive the current signal and convert the current signal into the voltage signal.

3. The wide-range fluorescence quantitative detection device as described in claim 2, characterized in that, The voltage conversion circuit includes a first amplifier, a first resistor, a first capacitor, a second capacitor, a third capacitor, and a fourth capacitor; The positive input terminal of the first amplifier is connected to the positive terminal of the photoelectric sensor, and the negative input terminal of the first amplifier is connected to the negative terminal of the photoelectric sensor; the first terminal of the first resistor is connected to the negative input terminal of the first amplifier, and the second terminal of the first resistor is connected to the output terminal of the first amplifier; the first capacitor is connected in parallel with the first resistor; the power supply terminal of the first amplifier is used to receive the power supply voltage and is grounded through the second capacitor; the first terminal of the third capacitor is connected to the positive terminal of the photoelectric sensor and receives a preset voltage, and the second terminal of the third capacitor is grounded; the fourth capacitor is connected in parallel with the third capacitor.

4. The wide-range fluorescence quantitative detection device as described in claim 1, characterized in that, The signal amplification circuit includes a first voltage follower circuit, a second voltage follower circuit, a first amplification circuit, and a second amplification circuit; The first voltage follower circuit is connected to the photoelectric conversion circuit and is used to receive the voltage signal and output it to the voltage acquisition circuit. The first amplifier circuit is connected to the photoelectric conversion circuit and is used to amplify the voltage signal by a first preset factor to obtain a first voltage amplified signal; The second voltage follower circuit is connected to the first amplifier circuit and is used to receive the first voltage amplification signal and output it to the voltage acquisition circuit. The second amplification circuit is connected to the first amplification circuit and is used to amplify the first voltage amplification signal by a second preset factor to obtain a second voltage amplification signal, and output it to the voltage acquisition circuit.

5. The wide-range fluorescence quantitative detection device as described in claim 4, characterized in that, The first voltage follower circuit includes a second amplifier, a second resistor, and a fifth capacitor; The positive input terminal of the second amplifier is connected to the photoelectric conversion circuit through the second resistor, the negative input terminal of the second amplifier is connected to the output terminal of the second amplifier, and the output terminal of the second amplifier is connected to the voltage acquisition circuit; the first terminal of the fifth capacitor is connected to the positive input terminal of the second amplifier, and the second terminal of the fifth capacitor is grounded.

6. The wide-range fluorescence quantitative detection device as described in claim 4, characterized in that, The first amplifier circuit includes a third amplifier, a third resistor, a fourth resistor, and a sixth capacitor; The positive input terminal of the third amplifier is connected to the photoelectric conversion circuit; the negative input terminal of the third amplifier is grounded through the third resistor and connected to the output terminal of the third amplifier through the fourth resistor; the power supply terminal of the third amplifier is used to receive the power supply voltage and is grounded through the sixth capacitor.

7. The wide-range fluorescence quantitative detection device as described in claim 4, characterized in that, The second follower circuit includes a fourth amplifier, a fifth resistor, and a seventh capacitor; The positive input terminal of the fourth amplifier is connected to the first amplifier circuit through the fifth resistor, the negative input terminal of the fourth amplifier is connected to the output terminal of the fourth amplifier, and the output terminal of the fourth amplifier is connected to the voltage acquisition circuit; the first terminal of the seventh capacitor is connected to the positive input terminal of the fourth amplifier, and the second terminal of the seventh capacitor is grounded.

8. The wide-range fluorescence quantitative detection device as described in claim 4, characterized in that, The second amplifier circuit includes a fifth amplifier, a sixth resistor, a seventh resistor, and an eighth capacitor; The positive input terminal of the fifth amplifier is connected to the first amplifier circuit; the negative input terminal of the fifth amplifier is grounded through the sixth resistor and connected to the output terminal of the fifth amplifier through the seventh resistor; the power supply terminal of the fifth amplifier is used to receive the power supply voltage and is grounded through the eighth capacitor.

9. The wide-range fluorescence quantitative detection device as described in claim 1, characterized in that, The controller is also used to obtain the concentration value corresponding to the sample based on n sets of voltage data and a preset regression equation.

10. A sample concentration detection device, characterized in that, Includes a wide fluorescence quantitative detection device as described in any one of claims 1 to 9.

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