Detection device combined with drug sensitive test

Abstract

The utility model provides a detection device combined with a drug sensitive test, which belongs to the technical field of medicine detection tools, and comprises a fixed plate, a plurality of connecting plates and a plurality of connecting plates, the detection piece is provided with a containing cavity for containing the bacterial suspension, the upper end face of the containing cavity is an opening, and a plurality of through holes are formed in the lower end face of the containing cavity and correspond to the grooves; an extrusion part is slidably arranged at the opening of the detection part in the vertical direction, and the extrusion part moves downwards by applying external force to discharge the bacterial suspension in the accommodating cavity into the groove; according to the utility model, the through holes are formed in the detection piece, and the number and the positions of the through holes are in one-to-one correspondence with those of the grooves of the fixed plate, so that the bacterial suspension is quickly discharged into the grooves in the fixed plate under the action of the extrusion piece.

Description

Technical Field

[0001] This utility model relates to the field of pharmaceutical testing tools, specifically to a detection device for combined drug sensitivity testing. Background Technology

[0002] The broth microdilution checkerboard method is a standard reference method for combined drug sensitivity testing. It can quantitatively detect multiple drugs and multiple concentration combinations, and calculate the fractional inhibitory concentration index (FICI) to determine the synergistic, additive, unrelated, or antagonistic effects of two drugs.

[0003] Chinese patent document CN110373447A describes a combined antimicrobial susceptibility test card for multidrug-resistant Gram-negative bacteria and its preparation method. It employs the principle of the broth microdilution checkerboard method to simultaneously detect the results of 9 single drugs and 14 combined antimicrobial susceptibility tests. It uses four 144-well antimicrobial susceptibility test cards containing combinations of different types and dilution gradients of antibiotics.

[0004] However, in practice, each well of these four drug sensitivity cards requires the manual addition of a certain volume of bacterial suspension. If this step is done manually, it means that the operator needs to inject the bacterial suspension into each well using a pipette. This process is not only tedious and lengthy but also prone to errors, greatly limiting its application in routine testing, especially for batch testing of large sample volumes, which is even more difficult. Utility Model Content

[0005] Therefore, the technical problem to be solved by this utility model is to overcome the defect in the prior art that requires operators to inject bacterial suspension into each well through a dropper during bacterial suspension testing, thereby providing a detection device for combined drug sensitivity testing.

[0006] To solve the above-mentioned technical problems, this utility model provides a detection device for combined drug sensitivity testing, including: a fixing plate, wherein the upper end surface of the fixing plate is provided with a plurality of grooves;

[0007] The detection element is provided with a cavity for containing bacterial suspension. The upper end face of the cavity is open, and the lower end face of the cavity is provided with a plurality of through holes, which correspond to the groove.

[0008] A squeezing element is slidably disposed at the opening of the detection element in the vertical direction. By applying external force, the squeezing element moves downward to discharge the bacterial suspension in the receiving cavity into the groove.

[0009] Preferably, the receiving cavity is provided with at least one partition groove, and the partition groove is provided with at least one recess.

[0010] Preferably, the diameter of the through hole gradually decreases from the side away from the groove toward the groove.

[0011] Preferably, a sealing element is provided between the outer end face of the extruder and the detection element.

[0012] Preferably, a guide groove or guide post is provided on the side end face of the receiving cavity, and a corresponding guide post or guide groove is provided on the extruder.

[0013] Preferably, a guide groove is provided on the outer end face of the receiving cavity.

[0014] Preferably, the lower end face of the detection element is provided with a plurality of extensions extending toward the groove, and the extensions are provided with liquid guiding channels, the inlet of the liquid guiding channels being connected to the through hole.

[0015] Preferably, the diameter of the extension gradually tapers from above toward the groove.

[0016] Preferably, a measuring scale is provided on the outer end face of the testing component.

[0017] The technical solution of this utility model has the following advantages:

[0018] 1. The detection device for combined drug sensitivity testing provided by this utility model, by setting through holes on the detection piece, the number and position of the through holes correspond one-to-one with the grooves of the fixed plate, so that the bacterial suspension is quickly discharged into the grooves of the fixed plate under the action of the extruder.

[0019] 2. The detection device for combined drug sensitivity testing provided by this utility model has a dividing groove that allows the containing cavity to form different independent spaces, thereby allowing different types and concentrations of antibiotics used for combined drug sensitivity testing to be pre-added to each dividing groove, and then adding the same concentration of bacterial suspension to different dividing grooves to obtain different test results.

[0020] 3. The detection device for combined drug sensitivity testing provided by this utility model allows the liquid to flow along the inclined surface toward the center of the orifice, avoiding excessive bacterial suspension remaining on the bottom surface of the receiving cavity. Attached Figure Description

[0021] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0022] Figure 1The front view of the detection device for combined drug susceptibility testing provided in the first embodiment of this utility model;

[0023] Figure 2 for Figure 1 Exploded view of the detection device for the combined drug susceptibility test;

[0024] Figure 3 for Figure 1 Exploded view of the detection device for the combined drug susceptibility test;

[0025] Figure 4 for Figure 1 The front view of the detection device for the combined drug susceptibility test provided in the second embodiment.

[0026] Explanation of reference numerals in the attached figures:

[0027] 1. Fixing plate; 2. Groove; 3. Detection piece; 4. Receiving cavity; 5. Through hole; 6. Extrusion piece; 7. Separating groove; 8. Sealing piece; 9. Guide groove; 10. Guide post; 11. Extension piece. Detailed Implementation

[0028] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0029] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0030] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0031] Furthermore, the technical features involved in the different embodiments of this utility model described below can be combined with each other as long as they do not conflict with each other.

[0032] Example 1

[0033] The detection device for combined drug sensitivity testing provided in this embodiment is used for detection by the broth micro-dilution checkerboard method.

[0034] like Figure 1 The diagram illustrates a specific embodiment of the combined drug susceptibility testing device provided in this example, comprising: a fixing plate 1, a testing element 3, and a squeezing element 6. The upper surface of the fixing plate 1 is provided with grooves 2 arranged in a 6*6 or 6*8 pattern; the testing element 3 is provided with a receiving cavity 4, into which the bacterial suspension can be stored. The upper surface of the receiving cavity 4 is open, facilitating the pouring of the bacterial suspension into the receiving cavity 4. The lower surface of the receiving cavity 4 is provided with several through holes 5, which penetrate the lower end of the testing element 3. The number of through holes 5 is consistent with the number of grooves 2, and the positions of the through holes 5 and grooves 2 correspond one-to-one. That is, the bacterial suspension in the receiving cavity 4 can flow along the through holes 5 into each groove 2 on the fixing plate 1 under the action of gravity; the squeezing element 6 is slidably disposed vertically at the opening of the testing element 3, sealing the squeezing element 6 with the testing element 3. By applying external force, the squeezing element 6 moves downward to discharge the bacterial suspension in the receiving cavity 4 into the grooves 2. In use, first place the fixing plate 1 horizontally, then place the detection piece 3 on top of the fixing plate 1, aligning the four edges of the detection piece 3 with the four edges of the fixing plate 1. At this point, the holes 5 and grooves 2 correspond vertically. Move the extruder 6 vertically upwards, removing it from the receiving cavity 4. Then, pour the bacterial suspension into the receiving cavity 4. Due to the small diameter of the holes 5, the liquid surface tension prevents it from flowing into the grooves 2 of the fixing plate 1. Reinstall the extruder 6 onto the detection piece 3, sealing its edge. Moving the extruder 6 downwards allows the bacterial suspension to drain into the grooves 2. By providing holes 5 on the detection piece 3, with the number and position of the holes corresponding to the grooves 2, the bacterial suspension is quickly drained into the grooves 2 on the fixing plate 1 under the action of the extruder 6.

[0035] like Figure 2As shown in this embodiment, the receiving cavity 4 is provided with multiple partition grooves 7, and each partition groove 7 is provided with multiple recesses 2. The partition grooves 7 enable the receiving cavity 4 to form different independent spaces, thereby allowing different types and concentrations of antibiotics used for combined drug sensitivity testing to be pre-added to each partition groove 7. Then, bacterial suspension of the same concentration is added to different partition grooves to obtain different test results. In addition, as an alternative embodiment, the number of partition grooves 7 is not limited, and those skilled in the art can modify it according to actual needs.

[0036] like Figure 2 As shown, this is one embodiment of the detection device for combined drug susceptibility testing provided in this example. The diameter of the orifice 5 gradually narrows from the top towards the groove 2. The larger diameter at the top facilitates the collection of more bacterial suspension, allowing the liquid to flow along the inclined surface towards the center of the orifice 5, avoiding excessive bacterial suspension residue on the bottom surface of the receiving cavity 4. Alternatively, as an alternative embodiment, the diameter of the orifice 5 can be set to a fixed value, and the bottom surface of the receiving cavity 4 can be set to an inclined surface.

[0037] like Figure 2 , Figure 3 As shown, this embodiment illustrates one implementation of the combined drug susceptibility testing device provided in this example. A sealing element 8 is provided between the outer end face of the extruder 6 and the test element 3. The extruder 6 is a rectangular plate, horizontally positioned within the receiving cavity 4, and the sealing element 8 is disposed on the four sides of the rectangular plate. The sealing element 8 is made of rubber and provides a seal between the extruder 6 and the receiving cavity 4, allowing the bacterial suspension to be discharged when the extruder 6 moves downwards. Alternatively, as an alternative implementation, the sealing element 8 can be made of other materials, such as polytetrafluoroethylene (PTFE). Furthermore, as an alternative implementation, if the extruder 6 is not equipped with a sealing element 8, the entire extruder 6 can be made of rubber, which itself provides a seal between it and the test element 3.

[0038] like Figure 2 As shown, this is one embodiment of the detection device for combined drug sensitivity testing provided in this example. The receiving cavity 4 is provided with a guide groove 9, and the extruder 6 is provided with a corresponding guide post 10. The guide post 10 is arranged vertically and slidably disposed within the guide groove 9, allowing the extruder 6 to move vertically with greater stability. Alternatively, as an alternative embodiment, the receiving cavity 4 can also be provided with a guide post 10, and the extruder 6 with a guide groove 9.

[0039] like Figure 2As shown, this embodiment illustrates one implementation of the detection device for combined drug sensitivity testing. Four guide grooves 9 are provided on the outer end face of the receiving cavity 4, and four guide posts 10 are provided on the extruder 6. In use, the four guide posts 10 are inserted into the guide grooves 9. When the extruder 6 moves downward, the guide posts 10 slide within the guide grooves 9, limiting the movement of the extruder 6. Alternatively, as an alternative implementation, the number of guide grooves 9 and guide posts 10 is not limited. For example, six or eight are acceptable.

[0040] like Figure 3 As shown, this embodiment illustrates one implementation of the combined drug susceptibility testing device. The lower end face of the detection element 3 is provided with several extensions 11 extending towards the groove 2. Each extension 11 has a liquid guiding channel, the inlet of which communicates with the through hole 5. The number and position of the extensions 11 correspond to the number and position of the through holes 5. In use, the extensions 11 extend into the groove 2, allowing for more precise flow of the bacterial suspension into the groove 2. Alternatively, as an alternative implementation, the extensions 11 can be omitted, and the bacterial suspension can flow directly into the groove 2 through the through hole 5.

[0041] like Figure 3 As shown, this is one embodiment of the detection device for combined drug susceptibility testing provided in this example. The diameter of the extension 11 gradually narrows from the top towards the groove 2. The larger diameter at the top facilitates the collection of more bacterial suspension; the smaller diameter at the bottom facilitates the guidance of the bacterial suspension, allowing it to flow into the groove 2. Alternatively, as an alternative embodiment, the diameter of the extension 11 can be set to a fixed value, which can also achieve the guiding effect on the bacterial suspension.

[0042] The reagent kit is installed as follows: The upper surface of the fixing plate 1 has several grooves 2. The detection element 3 has a receiving cavity 4, and the lower surface of the receiving cavity 4 has a through hole 5. The through hole 5 passes through the detection element 3, allowing the bacterial suspension in the receiving cavity 4 to flow into the groove 2 through the through hole 5 and the extension 11. A guide groove 9 is provided on the outer wall of the detection element 3, and a guide post 10 corresponding to and slidably connected to the guide groove 9 is provided on the extruder 6. A sealing element 8 is provided between the end faces of the extruder 6 and the receiving cavity 4. By pouring bacterial suspensions of different concentrations into different compartments 7 of the receiving cavity 4 (50-100 μL per compartment), the dosage of bacterial suspension in the groove 2 is slightly more than the capacity of each compartment. After aligning the guide post 10 of the extruder 6 with the guide groove 9 and inserting it, the extruder 6 is moved downwards. Because the extruder 6 is sealed to the receiving cavity 4, moving the extruder 6 will cause the bacterial suspension to be discharged into the groove 2.

[0043] Example 2

[0044] like Figure 4As shown, this embodiment is partially the same as Embodiment 1, except that the detection element 3 is made of a transparent material. A scale is provided on the outer end face of the detection element 3. After the extruder 6 is installed on the detection element 3, the amount of bacterial suspension discharged can be determined by observing the comparison between the moving distance of the extruder 6 and the scale. Dividing this by the number of holes 5 yields the dosage of bacterial suspension in each groove 2. The scale design makes the operation of the extruder 6 more convenient.

[0045] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the protection scope of this utility model.

Claims

1. A detection device for combined drug susceptibility testing, characterized in that, include: A fixing plate (1) is provided with a plurality of grooves (2) on its upper end surface; The test piece (3) is provided with a cavity (4) for containing bacterial suspension. The upper end face of the cavity (4) is open, and the lower end face of the cavity (4) is provided with a plurality of through holes (5). The through holes (5) correspond to the groove (2). The opening of the detection piece (3) is slidably provided with a squeezing member (6) in the vertical direction. Under the action of external force, the squeezing member (6) moves downward in the vertical direction to discharge the bacterial suspension in the receiving cavity (4) into the groove (2).

2. The detection device for combined drug susceptibility testing according to claim 1, characterized in that, The receiving cavity (4) is provided with at least one partition groove (7), and the partition groove (7) is provided with at least one groove (2).

3. The detection device for combined drug susceptibility testing according to claim 2, characterized in that, The diameter of the through hole (5) gradually decreases from the side away from the groove (2) toward the groove (2).

4. The detection device for combined drug susceptibility testing according to any one of claims 1-3, characterized in that, A sealing element (8) is provided between the outer end face of the extruder (6) and the detection element (3).

5. The detection device for combined drug susceptibility testing according to claim 4, characterized in that, The side end face of the receiving cavity (4) is provided with a guide groove (9) or a guide post (10), and the extruder (6) is provided with a corresponding guide post (10) or guide groove (9).

6. The detection device for combined drug susceptibility testing according to claim 5, characterized in that, A guide groove (9) is provided on the outer end face of the receiving cavity (4).

7. The detection device for combined drug susceptibility testing according to any one of claims 1-3, characterized in that, The lower end face of the detection element (3) is provided with a plurality of extensions (11) extending toward the groove (2), and the extensions (11) are provided with liquid guiding channels, the inlet of the liquid guiding channels being connected to the through hole (5).

8. The detection device for combined drug susceptibility testing according to claim 7, characterized in that, The diameter of the extension (11) gradually tapers from above toward the groove (2).

9. The detection device for combined drug susceptibility testing according to any one of claims 1-3, characterized in that, A measuring scale is provided on the outer end face of the testing component (3).

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