Efficient immune cell sampling device

Abstract

The utility model discloses an efficient immune cell sampling device and belongs to the technical field of immune cell sampling. The efficient immune cell sampling device comprises a sampling needle cylinder and a propeller located in the sampling needle cylinder, a first cavity is formed in the side wall of the sampling needle cylinder, a second cavity is formed in the bottom wall of the sampling needle cylinder, the first cavity is communicated with the second cavity, and a through hole communicated with the second cavity is formed in the bottom wall of the outer side of the sampling needle cylinder; an exhaust hole communicated with the first cavity is formed in the top of the sampling needle cylinder; the efficient immune cell sampling device further comprises a push plate, a liquid inlet pipe and a sealing piece, a sliding hole is formed in the middle of the push plate, the inner wall of the sliding hole is slidably connected with the outer wall of a needle tip on the sampling needle cylinder, and the circumferential outer wall of the push plate is slidably connected with the inner wall of the second cavity. According to the efficient immune cell sampling device, the problems that an existing sampling device needs to be stored in a low-temperature environment all the time, the requirement for the environment is high, the storage time is long, and the storage cost is increased are solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to immune cell sampling technical field, concretely relates to a kind of high-efficiency immune cell sampling device. BACKGROUND

[0002] Immune cells, commonly known as white blood cells, are a class of cells involved in or related to immune response, mainly including lymphocyte groups, monocytes, macrophages and dendritic cells, collectively referred to as peripheral blood mononuclear cells, which play an important role in stabilizing body health in the human body. It has important application potential in improving immunity, improving sub-health, anti-aging, anti-infection, anti-tumor and other aspects.

[0003] When the human body is diseased, it can supplement the immune cells with vitality into the human body for precise cell therapy to improve the body's immunity and help health recovery.

[0004] However, in order to ensure the activity of immune cells, immune cells are usually stored in a liquid nitrogen tank at-196℃, so that the immune cells are in a dormant state, and the immune cells are extracted from the liquid nitrogen tank when needed and recovered for use in the human body.

[0005] In the extraction process, in order to ensure the activity of immune cells, an extraction device with cold preservation is usually used. However, the immune cell sampling device disclosed in CN220079069U requires to be stored in a low-temperature cold storage or refrigerator before use, so that the temperature of the refrigeration cavity in the device is reduced to a suitable temperature, and the specific suitable temperature can be determined according to the required refrigeration temperature of the immune cells. Although the above prior art can preserve cold, the immune cell sampling device needs to be stored in a low-temperature environment at all times, which requires a high environment and a long storage time, increasing the storage cost. UTILITY MODEL CONTENT

[0006] The utility model aims to overcome the problems in the prior art, and provides a high-efficiency immune cell sampling device that only needs to be stored normally. When the immune cells need to be sampled, coolants can be injected into the liquid inlet pipe in advance, avoiding the need to store the existing sampling device in a low-temperature environment at all times, and avoiding the problems of high environmental requirements, long storage time and increased storage cost.

[0007] The utility model provides a kind of efficient immune cell sampling device including sampling needle cylinder and located in the pusher of sampling needle cylinder, the lateral wall of sampling needle cylinder has first cavity, the bottom wall of sampling needle cylinder has second cavity, first cavity is communicated with second cavity, the outside bottom wall of sampling needle cylinder is equipped with the perforation being communicated with the second cavity, the top of sampling needle cylinder is equipped with the exhaust hole being communicated with the first cavity;The efficient immune cell sampling device further includes: push plate, liquid inlet pipe and closure;Push plate middle part has sliding hole, the inner wall of sliding hole is slidably connected on the needle tip outer wall of the sampling needle cylinder, the circumferential outer wall of push plate is slidably connected with the inner wall of the second cavity;Liquid inlet pipe is arranged on the outer wall of sampling needle cylinder, and is communicated with the first cavity, for injecting coolant into first cavity;Closure is arranged on liquid inlet pipe, for blocking liquid inlet pipe.

[0008] Preferably, the bottom surface of the push plate is fixedly connected with a connecting rod, the length direction of the connecting rod is parallel to the axis of the sampling needle cylinder, the connecting rod passes through the perforation and is fixedly connected with the bottom wall of the sleeve, the sleeve is sleeved on the sampling needle cylinder, and the sleeve is threadedly connected with the sampling needle cylinder.

[0009] Preferably, the number of the connecting rods is multiple, the multiple connecting rods are uniformly arranged around the axis of the sampling needle cylinder, and one of the connecting rods is provided with a through hole in the length direction of the connecting rod, one end of the through hole is communicated with the discharge hole on the push plate, and the other end of the through hole is inserted with a first plug body.

[0010] Preferably, the end inner wall of the exhaust hole is provided with a waterproof and breathable film, the top of the sampling needle cylinder is provided with an isolation cover, the inner wall of the isolation cover is communicated with the exhaust hole, and the outlet of the isolation cover is away from the axis of the sampling needle cylinder.

[0011] Preferably, the closure is a second plug body, and the second plug body is inserted into the end of the liquid inlet pipe.

[0012] Preferably, the opposite surfaces of the push plate and the second cavity are both conical surfaces, and the two conical surfaces have the same taper angle.

[0013] Preferably, the side wall of the sliding hole on the push plate is provided with multiple first limiting ring grooves, a first rubber sealing ring is arranged in the first limiting ring groove away from the pusher, a first arc-shaped metal strip is arranged in each of the remaining first limiting ring grooves, and the first arc-shaped metal strip is slidably connected with the needle tip outer wall of the sampling needle cylinder.

[0014] Preferably, the circumferential surface of the push plate is provided with multiple second limiting ring grooves, a second rubber sealing ring is arranged in the second limiting ring groove away from the pusher, a second arc-shaped metal strip is arranged in each of the remaining second limiting ring grooves, and the second arc-shaped metal strip is slidably connected with the side wall of the second cavity.

[0015] Preferably, the pusher comprises a piston head and a push rod, the piston head is slidingly connected with the inner wall of the sampling needle cylinder, the piston head is connected with the push rod, and a scale is arranged on the push rod.

[0016] Compared with the prior art, the utility model has the beneficial effects that:

[0017] In the high-efficiency immune cell sampling device, the push plate is located at the end of the second cavity away from the pusher, cooling liquid (low-temperature liquid) is injected into the first cavity and the second cavity through the liquid inlet pipe, then the liquid inlet pipe is blocked by the sealing element, the push plate is moved towards the pusher, the push plate extrudes the cooling liquid in the second cavity into the first cavity, so that the first cavity is filled with cooling liquid, and the air in the first cavity is discharged from the exhaust hole during the movement, and the cooling liquid is distributed in the first cavity, so that the inner wall of the sampling needle cylinder can be fully cooled.

[0018] The high-efficiency immune cell sampling device only needs to be stored normally, and when the immune cells need to be sampled, the cooling liquid can be injected into the liquid inlet pipe in advance, the storage environment requirement of the high-efficiency immune cell sampling device is low, and the storage cost is lower. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a perspective view of the utility model;

[0020] Figure 2 It is a sectional view of the utility model;

[0021] Figure 3 It is a sectional view of the sampling needle cylinder;

[0022] Figure 4 It is a schematic view of the push plate in the utility model;

[0023] Figure 5 It is a sectional view of the sleeve in the utility model;

[0024] Figure 6 It is an assembly view of the push plate;

[0025] Figure 7 It is a sectional view of Figure 6 ;

[0026] Figure 8 It is a sectional view of Figure 7 ;

[0027] Figure 9 It isFigure 7 Close-up view at B;

[0028] Figure 10 Enlarged view of the pusher;

[0029] Figure 11 Modeling schematic view of the utility model.

[0030] Explanation of reference signs:

[0031] 1. Sampling needle cylinder, 2. Pusher, 3. First cavity, 4. Second cavity, 5. Perforation, 6. Push plate, 7. Slide hole, 8. Liquid inlet pipe, 9. Connecting rod, 10. Sleeve, 11. Through hole, 12. Discharge hole, 13. Exhaust hole, 14. Isolation cover, 15. First rubber sealing ring, 16. First arc-shaped metal strip, 17. Second rubber sealing ring, 18. Second arc-shaped metal strip, 19. Piston head, 20. Push rod, 21. Scale. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme of the embodiments of the utility model will be described clearly and completely below in combination with the drawings of the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. Based on the described embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0033] Unless otherwise defined, the technical terms or scientific terms used herein should be understood as the usual meanings understood by those skilled in the art to which the utility model belongs. "Include" or "contain" and similar words mean that the elements or objects appearing before the words cover the elements or objects listed after the words and their equivalents, without excluding other elements or objects. "In", "out", "up", "down", "far", "near", "front", "back" and the like are only used to indicate relative positional relationships, which may change accordingly when the absolute positions of the described objects change.

[0034] The drawings in the utility model are not strictly drawn according to the actual proportions, and the specific sizes and quantities of various structures can be determined according to actual needs. The drawings described in the utility model are only structural schematic views.

[0035] The high-efficiency immune cell sampling device provided by the utility model is Figures 1-4 and Figure 11The device includes a sampling syringe 1 and a pusher 2 located inside the sampling syringe 1. The sampling syringe 1 has a first cavity 3 on its side wall and a second cavity 4 on its bottom wall. The first cavity 3 and the second cavity 4 are connected. The outer bottom wall of the sampling syringe 1 is provided with a perforation 5 that communicates with the second cavity 4. The top of the sampling syringe 1 is provided with an exhaust port 13 that communicates with the first cavity 3. The high-efficiency immune cell sampling device also includes a push plate 6, an inlet tube 8, and a sealing member. The push plate 6 has a sliding hole 7 in the middle. The inner wall of the sliding hole 7 is slidably connected to the outer wall of the needle tip on the sampling syringe 1. The circumferential outer wall of the push plate 6 is slidably connected to the inner wall of the second cavity 4. The inlet tube 8 is disposed on the outer wall of the sampling syringe 1 and communicates with the first cavity 3 for injecting coolant into the first cavity 3. The sealing member is disposed on the inlet tube 8 for blocking the inlet tube 8.

[0036] The pusher plate 6 is located at the end of the second cavity 4 away from the pusher 2. Coolant (low-temperature liquid) is injected into the first cavity 3 and the second cavity 4 through the liquid inlet pipe 8. Then, the liquid inlet pipe 8 is sealed by the sealing member, and the pusher plate 6 is moved toward the pusher 2. The pusher plate 6 squeezes the coolant in the second cavity 4 into the first cavity 3, so that the first cavity 3 is filled with coolant. During this process, the air in the first cavity 3 is discharged from the exhaust port 13. The coolant is distributed in the first cavity 3, which can fully cool the inner wall of the sampling syringe 1. When sampling immune cells from liquid nitrogen using this high-efficiency immune cell sampling device, the needle tip on the sampling syringe 1 is inserted into the liquid nitrogen, and the pusher 2 is dragged out of the sampling syringe 1. The immune cell sample enters the sampling syringe 1. Since the inner wall of the sampling syringe 1 has been cooled by the coolant, the immune cell sample can maintain high activity when it enters the sampling syringe 1.

[0037] This high-efficiency immune cell sampling device only needs to be stored normally. When it is needed to use and sample immune cells, coolant can be injected into the inlet tube 8 in advance. This high-efficiency immune cell sampling device has low requirements for the storage environment and lower storage costs.

[0038] In this embodiment, as Figure 2 and Figure 5 A connecting rod 9 is fixedly connected to the bottom surface of the push plate 6. The length direction of the connecting rod 9 is parallel to the axis of the sampling syringe 1. The connecting rod 9 passes through the through hole 5 and is fixedly connected to the bottom wall of the sleeve 10. The sleeve 10 is sleeved on the sampling syringe 1 and is threadedly connected to the sampling syringe 1.

[0039] The outer circumference of the sleeve 10 has anti-slip texture. Rotating the sleeve 10 can achieve the purpose of pushing the push plate 6 through the connecting rod 9.

[0040] In this embodiment, as Figure 2 and Figures 4-6The number of the connecting rods 9 is multiple, the multiple connecting rods 9 are uniformly arranged around the axis of the sampling needle cylinder 1, and one of the connecting rods 9 is provided with a through hole 11 along the length direction of the connecting rod 9, one end of the through hole 11 is communicated with the exhaust hole 12 on the push plate 6, and the other end of the one end of the through hole 11 is inserted with a first plug body.

[0041] After the high-efficiency immune cell sampling device is used and the immune cell sample liquid is squeezed out, the first plug body is removed, so that the cooling liquid in the first cavity 3 and the second cavity 4 is discharged.

[0042] In the embodiment, the push plate 6 is provided with a sliding hole 7, the sliding hole 7 is communicated with the second cavity 4, and the sliding hole 7 is provided with a plurality of first limiting ring grooves. Figures 1-3 The end inner wall of the exhaust hole 13 is provided with a waterproof and breathable film, the top of the sampling needle cylinder 1 is provided with an isolation cover 14, the inner wall of the isolation cover 14 is communicated with the exhaust hole 13, and the outlet of the isolation cover 14 is away from the axis of the sampling needle cylinder 1.

[0043] The waterproof and breathable film is an existing product, which can prevent the push plate 6 from moving too fast or too much to cause the cooling liquid in the first cavity 3 to be squeezed out of the exhaust hole 13, and at the same time allows the air in the first cavity 3 to be discharged from the first cavity 3, the isolation cover 14 is used to avoid the low-temperature gas discharged from the first cavity 3 from contacting the human body, and the safety of the operator is improved.

[0044] In the embodiment, the push plate 6 is provided with a sliding hole 7, the sliding hole 7 is communicated with the second cavity 4, and the sliding hole 7 is provided with a plurality of first limiting ring grooves. Figure 1 The sealing member is a second plug body, and the second plug body is inserted into the end of the liquid inlet pipe 8.

[0045] The second plug body adopts a rubber plug, which can be screwed into the liquid inlet pipe 8 or directly inserted into the liquid inlet pipe 8, as long as it can block the liquid inlet pipe 8.

[0046] In the embodiment, the push plate 6 is provided with a sliding hole 7, the sliding hole 7 is communicated with the second cavity 4, and the sliding hole 7 is provided with a plurality of first limiting ring grooves. Figure 2 and Figure 7 The push plate 6 and the opposite surface of the second cavity 4 are both conical surfaces, and the two conical surfaces have the same cone angle.

[0047] After the cooling liquid enters the second cavity 4, the conical surface can accelerate the upward movement of the air in the second cavity 4, avoiding the problem of residual air in the second cavity 4.

[0048] In the embodiment, the push plate 6 is provided with a sliding hole 7, the sliding hole 7 is communicated with the second cavity 4, and the sliding hole 7 is provided with a plurality of first limiting ring grooves. Figures 7-8 The side wall of the sliding hole 7 on the push plate 6 is provided with a plurality of first limiting ring grooves, a first rubber sealing ring 15 is arranged in the first limiting ring groove away from the pusher 2, a first arc-shaped metal strip 16 is arranged in each of the remaining first limiting ring grooves, and the first arc-shaped metal strip 16 is slidably connected to the outer wall of the needle tip of the sampling needle cylinder 1.

[0049] The directions of the notches of the two adjacent first arc-shaped metal strips 16 are not coincident, preferably opposite, the inner surfaces of the first arc-shaped metal strips 16 abut against the outer wall of the needle tip of the sampling needle cylinder 1 for preventing leakage, the first rubber sealing ring 15 is the last line of defense and is farthest from the cooling liquid in the second cavity 4, so that the influence of low temperature on the first rubber sealing ring 15 is reduced to the maximum, and the first rubber sealing ring 15 keeps the best sealing effect.

[0050] In the embodiment, the pusher 2 comprises a piston head 19 and a push rod 20, the piston head 19 is slidably connected to the inner wall of the sampling needle cylinder 1, the piston head 19 is connected to the push rod 20, and the push rod 20 is provided with a scale 21. Figure 7 Figure 9 The circumferential surface of the push plate 6 is provided with a plurality of second limiting ring grooves, a second rubber sealing ring 17 is arranged in the second limiting ring groove away from the pusher 2, and a second arc-shaped metal strip 18 is arranged in each of the remaining second limiting ring grooves, and the second arc-shaped metal strip 18 is slidably connected to the side wall of the second cavity 4.

[0051] The directions of the notches of the two adjacent second arc-shaped metal strips 18 are not coincident, preferably opposite, the outer surfaces of the second arc-shaped metal strips 18 abut against the inner wall of the second cavity 4 for preventing leakage, the second rubber sealing ring 17 is the last line of defense and is farthest from the cooling liquid in the second cavity 4, so that the influence of low temperature on the second rubber sealing ring 17 is reduced to the maximum, and the second rubber sealing ring 17 keeps the best sealing effect.

[0052] In the embodiment, the pusher 2 comprises a piston head 19 and a push rod 20, the piston head 19 is slidably connected to the inner wall of the sampling needle cylinder 1, the piston head 19 is connected to the push rod 20, and the push rod 20 is provided with a scale 21. Figure 10

[0053] The use method of the efficient immune cell sampling device is as follows:

[0054] Firstly, the sleeve 10 is reversely rotated, so that the push plate 6 is farthest from the pusher 2;

[0055] Secondly, the first plug body is confirmed to block the through hole 11, the piston head 19 is located at the innermost side of the sampling needle cylinder 1, the second plug body is removed, an appropriate amount of cooling liquid is injected into the liquid inlet pipe 8, and then the second plug body is used to block the liquid inlet pipe 8;

[0056] Then, the sleeve 10 is forwardly rotated, and the push plate 6 extrudes the cooling liquid in the second cavity 4 into the first cavity 3;

[0057] Next, the needle tip on the sampling needle cylinder 1 is stretched into liquid nitrogen, the pusher 2 is dragged outwards from the sampling needle cylinder 1, the immune cell sample liquid enters the sampling needle cylinder 1, and sampling is realized;

[0058] Finally, after sampling is completed, the first plug body is removed, the through hole 11 is opened, and after the cooling liquid is completely discharged, the first plug body can be installed.​​

[0059] The basic principle, main features and advantages of the present application are shown and described above. The skilled person in the art should understand that the present application is not limited by the above embodiments, and the above embodiments and descriptions in the specification are only preferred examples of the present application and are not intended to limit the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. A high efficiency immune cell sampling device comprising a sampling needle cylinder (1) and a pusher (2) located inside the sampling needle cylinder (1), characterized in that, The side wall of the sampling needle cylinder (1) has a first cavity (3), the bottom wall of the sampling needle cylinder (1) has a second cavity (4), the first cavity (3) and the second cavity (4) are communicated, the outer bottom wall of the sampling needle cylinder (1) is provided with a through hole (5) communicated with the second cavity (4), and the top of the sampling needle cylinder (1) is provided with an exhaust hole (13) communicated with the first cavity (3); The high-efficiency immune cell sampling device further comprises: The push plate (6) has a sliding hole (7) in the middle, the inner wall of the sliding hole (7) is in sliding connection with the outer wall of the needle tip on the sampling needle cylinder (1), and the circumferential outer wall of the push plate (6) is in sliding connection with the inner wall of the second cavity (4); The liquid inlet pipe (8) is arranged on the outer wall of the sampling needle cylinder (1) and is communicated with the first cavity (3) and is used for injecting cooling liquid into the first cavity (3); The closing piece is arranged on the liquid inlet pipe (8) and is used for blocking the liquid inlet pipe (8).

2. The high efficiency immune cell sampling device of claim 1, wherein, The bottom surface of the push plate (6) is fixedly connected with a connecting rod (9), the length direction of the connecting rod (9) is parallel to the axis of the sampling needle cylinder (1), the connecting rod (9) penetrates through the through hole (5) and is fixedly connected with the bottom wall of a sleeve (10), the sleeve (10) is sleeved on the sampling needle cylinder (1), and the sleeve (10) is in threaded connection with the sampling needle cylinder (1).

3. A high efficiency immune cell sampling device as claimed in claim 2, wherein, The number of the connecting rods (9) is multiple, the multiple connecting rods (9) are uniformly arranged around the axis of the sampling needle cylinder (1), and one of the connecting rods (9) is provided with a through hole (11) in the length direction of the connecting rod (9), one end of the through hole (11) is communicated with the discharge hole (12) on the push plate (6), and the other end of one end of the through hole (11) is inserted with a first plug body.

4. The high efficiency immune cell sampling device of claim 1, wherein, The end inner wall of the exhaust hole (13) is provided with a waterproof and breathable film, the top of the sampling needle cylinder (1) is provided with an isolation cover (14), the inner wall of the isolation cover (14) is communicated with the exhaust hole (13), and the outlet of the isolation cover (14) is away from the axis of the sampling needle cylinder (1).

5. The high efficiency immune cell sampling device of claim 1, wherein, The closing piece is a second plug body, and the second plug body is inserted into the end of the liquid inlet pipe (8).

6. The high efficiency immune cell sampling device of claim 1, wherein, The opposite surfaces of the push plate (6) and the second cavity (4) are both conical surfaces, and the cone angles of the two conical surfaces are the same.

7. The high efficiency immune cell sampling device of claim 1, wherein, The side wall of the sliding hole (7) on the push plate (6) is provided with multiple first limiting ring grooves, a first rubber sealing ring (15) is arranged in the first limiting ring groove away from the pusher (2), a first arc-shaped metal strip (16) is arranged in each of the remaining first limiting ring grooves, and the first arc-shaped metal strip (16) is in sliding connection with the outer wall of the needle tip on the sampling needle cylinder (1).

8. The high efficiency immune cell sampling device of claim 1, wherein, A plurality of second limiting ring grooves are arranged on the circumferential surface of the push plate (6), a second rubber sealing ring (17) is arranged in the second limiting ring groove away from the pusher (2), a second arc-shaped metal strip (18) is arranged in each of the remaining second limiting ring grooves, and the second arc-shaped metal strip (18) is in sliding connection with the side wall of the second cavity (4).

9. The high efficiency immune cell sampling device of claim 1, wherein, The propeller (2) comprises a piston head (19) and a push rod (20), the piston head (19) is slidingly connected to the inner wall of the sampling needle cylinder (1), the piston head (19) is connected to the push rod (20), and the push rod (20) is provided with a scale (21).

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