Active protein purification device

Abstract

The utility model provides an active protein purification device, which belongs to the technical field of experimental equipment manufacturing and comprises a bearing mechanism, a cylinder body, a cylinder cover movably connected to the end part of the cylinder body, and a fixed block fixedly mounted on the surface of the cylinder body. Through the arrangement of the bearing mechanism, the blanking mechanism and the connecting assembly, the efficient, convenient and reliable protein purification process is realized, the barrel body and the barrel cover of the bearing mechanism ensure the leakproofness of the purification process, the sampling assembly is convenient for real-time sampling detection, and the design of a rubber block and a fixing ring ensures that a system after sampling is still kept sealed, so that the sampling efficiency is improved. A regulating valve of the discharging mechanism accurately controls the discharging speed to adapt to different purification requirements, a press fit part and an auxiliary part in a connecting assembly enable replacement of a filter sieve to be simple and rapid, shutdown is not needed, the design of a limiting groove and the filter sieve improves the filtering efficiency, and the cooperation of a top block and a rotary knob enables maintenance of the filter sieve to be more convenient.

Description

TECHNICAL FIELD

[0001] The utility model belongs to experimental apparatus manufacturing technical field, concretely relates to a kind of active protein's purification device. BACKGROUND

[0002] Active protein purification technology originated in the early 20th century, gradually matured with the development of biochemistry and molecular biology, and its background technology mainly includes various physical and chemical separation methods, such as gel filtration, ion exchange, affinity chromatography, etc. Its development process has experienced a process from simple to complex and from low resolution to high resolution. Today, this technology is used in the production of therapeutic protein drugs in the pharmaceutical field, the extraction of functional factors in the food industry, and the development of biological products in the agricultural field, and has important significance for improving human health and quality of life.

[0003] The document with application number 201521093706.3 proposes a protein purification column, and the utility model discloses a protein purification column, which comprises a cylinder, a filter screen, a flow rate control valve and a plastic hose. The filter screen is arranged at the bottom of the cylinder, the bottom end of the cylinder is connected with the top end of the plastic hose, and the flow control valve is installed on the plastic hose. The utility model can realize the controllability of flow rate in the protein purification process, effectively improve the protein purification rate and speed.

[0004] In practical application, although the protein purification device mentioned in the comparative document can effectively separate and purify target proteins from mixtures, it has a significant disadvantage. The filter screen in the device is fixedly installed inside the device. Although this design ensures the stability of the filtration process to a certain extent, it also poses a certain risk. Once the filter screen is blocked due to the accumulation of protein impurities or solid particles during use, the entire device will be unusable. Therefore, an active protein purification device is needed. UTILITY MODEL CONTENTS

[0005] The utility model aims to provide an active protein purification device to solve the problems raised in the background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions:

[0007] An active protein purification device, comprising,

[0008] The bearing mechanism comprises a cylinder, a cylinder cover movably connected to the end of the cylinder, a fixed block fixedly installed on the surface of the cylinder, a through hole provided on the side wall of the cylinder, a sampling assembly provided on the inner wall of the through hole, and a connecting assembly provided at the bottom of the cylinder.

[0009] The blanking mechanism comprises a blanking hopper, a blanking hose communicated with the bottom of the blanking hopper, and an adjusting valve communicated with the end of the blanking hose.

[0010] As a preferred scheme of the utility model, the sampling assembly comprises a sampling pipe adaptedly installed on the inner wall of the through hole, and a sampling interface communicated with the end of the sampling pipe.

[0011] As a preferred scheme of the utility model, the sampling assembly further comprises a rubber block movably connected in the inner cavity of the sampling interface, and a fixing ring sleeved on the side wall of the sampling interface.

[0012] As a preferred scheme of the utility model, the connecting assembly comprises a pressing component, and an auxiliary component arranged below the pressing component and matched with the pressing component.

[0013] As a preferred scheme of the utility model, the pressing component comprises a pressing base, an auxiliary groove opened in the bottom of the pressing base, and a gasket fixedly installed on the bottom of the pressing base.

[0014] As a preferred scheme of the utility model, the pressing component further comprises a limiting groove opened in the side wall of the pressing base, and a filter screen movably connected on the side wall of the gasket.

[0015] As a preferred scheme of the utility model, the auxiliary component comprises a top base, a tension spring fixedly connected in the inner cavity of the top base, a top block movably connected on the side wall of the top base, and a knob fixedly installed on the side wall of the top block.

[0016] Compared with the prior art, the utility model has the beneficial effects that: through the arrangement of the bearing mechanism, the blanking mechanism and the connecting assembly, the efficient, convenient and reliable protein purification process is realized, the cylinder and the cylinder cover of the bearing mechanism ensure the airtightness of the purification process, the sampling assembly is convenient for real-time sampling detection, and the design of the rubber block and the fixing ring ensures that the system after sampling still maintains sealing, the adjusting valve of the blanking mechanism accurately controls the blanking speed, adapts to different purification requirements, the pressing component and the auxiliary component in the connecting assembly make the replacement of the filter screen simple and fast, without stopping, the design of the limiting groove and the filter screen improves the filtering efficiency, and the cooperation of the top block and the knob makes the maintenance of the filter screen more convenient. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical scheme of the embodiments of the utility model, the drawings needed to be used in the embodiment description will be briefly introduced below, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can be obtained according to these drawings without paying the creative labor. Among them:

[0018] Fig. 1 It is the whole structure schematic view of the utility model;

[0019] Fig. 2 It is the cylinder and cylinder cover connection schematic view of the utility model;

[0020] Fig. 3 It is the sampling tube and sampling interface connection schematic view of the utility model;

[0021] Fig. 4 It is the gasket and pressure seat connection schematic view of the utility model.

[0022] In the figure: 100, bearing mechanism;101, cylinder;102, cylinder cover;103, fixed block;104, through hole;105, sampling assembly;105a, sampling tube;105b, sampling interface;105c, rubber block;105d, fixed ring;106, connecting assembly;106a, pressure component;106a-1, pressure seat;106a-2, auxiliary groove;106a-3, gasket;106a-4, limiting groove;106a-5, filter screen;106b, auxiliary component;106b-1, top seat;106b-2, tension spring;106b-3, top block;106b-4, knob;200, blanking mechanism;201, blanking hopper;202, blanking hose;203, regulating valve. Specific embodiments

[0023] In order to make the above-mentioned purpose, features and advantages of the utility model more obvious and easy to understand, the specific embodiments of the utility model are described in detail below with the help of the accompanying drawings of the specification.

[0024] In the following description, a lot of specific details are set forth in order to facilitate a thorough understanding of the utility model, but the utility model can also be implemented in other ways different from the description herein, and those skilled in the art can make similar generalization without departing from the connotation of the utility model, therefore the utility model is not limited by the specific embodiments disclosed below.

[0025] Secondly, the "one embodiment" or "embodiment" referred to here means that the specific features, structures or characteristics can be included in at least one implementation of the utility model. "In one embodiment" does not mean the same embodiment in different places in this specification, nor is it an independent or alternative embodiment that excludes other embodiments.

[0026] Embodiments

[0027] Reference Figs. 1-4 For the utility model embodiment, the embodiment provides a kind of purification device of active protein, comprising,

[0028] The bearing mechanism 100 comprises a cylinder 101, a cylinder cover 102 movably connected to the end of the cylinder 101, a fixed block 103 fixedly installed on the surface of the cylinder 101, a through hole 104 arranged on the side wall of the cylinder 101, a sampling assembly 105 arranged on the inner wall of the through hole 104, and a connecting assembly 106 arranged at the bottom of the cylinder 101.

[0029] The discharging mechanism 200 comprises a discharging hopper 201, a discharging hose 202 communicated at the bottom of the discharging hopper 201, and an adjusting valve 203 communicated at the end of the discharging hose 202.

[0030] Specifically, the discharging hose 202 cooperates with the adjusting valve 203, so that the flow rate of the liquid in the discharging hose 202 can be adjusted, thereby controlling the speed of purification.

[0031] The sampling assembly 105 comprises a sampling pipe 105a adaptedly installed on the inner wall of the through hole 104, and a sampling interface 105b communicated at the end of the sampling pipe 105a. The sampling assembly 105 further comprises a rubber block 105c movably connected to the inner cavity of the sampling interface 105b, and a fixed ring 105d sleeved on the side wall of the sampling interface 105b.

[0032] Further, the rubber block 105c is arranged to facilitate the use of a needle cylinder to sample the liquid in the device and detect the purification effect in the device. The fixed ring 105d can be removed to facilitate replacement of the rubber block 105c.

[0033] The connecting assembly 106 comprises a pressing component 106a and an auxiliary component 106b arranged below the pressing component 106a for use in cooperation with the pressing component 106a. The pressing component 106a comprises a pressing seat 106a-1, an auxiliary groove 106a-2 opened at the bottom of the pressing seat 106a-1, and a gasket 106a-3 fixedly installed at the bottom of the pressing seat 106a-1. The pressing component 106a further comprises a limiting groove 106a-4 opened at the side wall of the pressing seat 106a-1, and a filter screen 106a-5 movably connected to the side wall of the gasket 106a-3.

[0034] Preferably, the auxiliary groove 106a-2 is arranged to facilitate pressing with the auxiliary component 106b. The gasket 106a-3 is made of rubber material to ensure the airtightness of the device during pressing. The filter screen 106a-5 can be removed and replaced when the pressing assembly is opened, and the filter screen 106a-5 is fixed when the pressing assembly is closed.

[0035] The auxiliary component 106b comprises a top seat 106b-1, a tension spring 106b-2 fixedly connected to the inner cavity of the top seat 106b-1, a top block 106b-3 movably connected to the side wall of the top seat 106b-1, and a knob 106b-4 fixedly installed on the side wall of the top block 106b-3.

[0036] It should be noted that when the top block 106b-3 is rotated by 90 degrees, the compression assembly can be pried open, and the tension spring 106b-2 pulls the compression assembly, and when the top block 106b-3 returns to the original position, the tension spring 106b-2 can pull the compression assembly back to the original position.

[0037] In use, the protein is placed in the barrel 101, the barrel cover 102 is covered, the flow rate is controlled by adjusting the valve 203, and during purification, a syringe can be inserted into the sampling interface 105b to extract the liquid sample in the barrel 101 for quality detection. The rubber block 105c ensures that the barrel 101 is still sealed after the syringe is extracted. When the filter screen 106a-5 is blocked, the knob 106b-4 can be actuated to rotate the top block 106b-3, which pries open the compression assembly and the auxiliary assembly. The filter screen 106a-5 loses its limit and can be removed and replaced with a new filter screen 106a-5. After replacement, the knob 106b-4 is actuated to return the clamp block to its original position, and the tension spring 106b-2 pulls the compression assembly back to its original position. The fixed block 103 is provided to facilitate clamping and fixing of the barrel 101.

[0038] In summary, efficient and convenient operation and maintenance are achieved. The close cooperation between the barrel 101, the adjusting valve 203, the sampling interface 105b, the rubber block 105c, the knob 106b-4, the top block 106b-3, the compression assembly, the auxiliary assembly, and the fixed block 103 in the device ensures accurate control of flow rate during purification, real-time sampling detection, and maintenance of the device's airtightness. When the filter screen 106a-5 is blocked, the linkage of the knob 106b-4 and the top block 106b-3 allows quick and convenient replacement of the filter screen 106a-5 without the need for complex maintenance, improving the device's efficiency and reliability while reducing the difficulty of operation and maintenance costs.

[0039] It is important to note that the construction and arrangements of the application shown in the various exemplary embodiments are illustrative only. Although only a few embodiments have been described in detail in this disclosure, those skilled in the art who review this disclosure will readily appreciate that many modifications can be made to the embodiments without departing from the novel teachings and advantages of the subject matter described herein (e.g., variations in sizes, dimensions, structures, shapes and proportions of the various elements, values of parameters, mounting arrangements, use of materials, colors, orientations, locations, and the like). For example, the elements shown as integrally formed can be constructed of multiple parts or elements, the position of elements can be reversed or otherwise varied, and the nature or number of elements or positions can be modified or changed. Accordingly, all such modifications are intended to be included within the scope of the present inventive subject matter. The order or sequence of any process or method steps can be varied or re-sequenced without departing from the subject matter described herein. Any "means plus function" clauses are intended to cover the structures described herein as performing the recited functions and not only structural equivalents but also equivalent structures. Other substitutions, modifications, changes, and omissions can be made in the design, operating conditions, and arrangement of the exemplary embodiments without departing from the scope of the present inventive subject matter. Accordingly, the present inventive subject matter is not limited to the particular embodiments described and illustrated herein, but extends to equivalents of which the foregoing describes are intended to cover.

[0040] Also, in an effort to provide a concise description of the exemplary embodiments, all features of an actual implementation can not be described (i.e., those unrelated to the presently contemplated best mode of carrying out the present inventive subject matter, or those unrelated to enabling the claimed application).

[0041] It should be appreciated that in the development of any actual implementation, as in any engineering or design project, numerous implementation-specific decisions can be made. Such development efforts might be complex and time-consuming, but would nevertheless be a routine undertaking of design, fabrication, and manufacture for those of ordinary skill having the benefit of this disclosure, without undue experimentation.

[0042] It should be noted that the above-mentioned embodiments are only used to illustrate the technical solutions of the present application, not limit the present application. Although the present application is described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application, and they should be covered in the scope of the claims of the present application.

Claims

1. A purification apparatus for an active protein, characterized in that: include, The support mechanism (100) includes a cylinder (101), a cylinder cover (102) movably connected to the end of the cylinder (101), a fixing block (103) fixedly installed on the surface of the cylinder (101), a through hole (104) provided on the side wall of the cylinder (101), a sampling component (105) provided on the inner wall of the through hole (104), and a connecting component (106) provided at the bottom of the cylinder (101). The feeding mechanism (200) includes a hopper (201), a feeding hose (202) connected to the bottom of the hopper (201), and a regulating valve (203) connected to the end of the feeding hose (202).

2. The purification apparatus for an active protein according to claim 1, characterized in that: The sampling assembly (105) includes a sampling tube (105a) adapted to be installed on the inner wall of the through hole (104), and a sampling interface (105b) connected to the end of the sampling tube (105a).

3. The purification apparatus for an active protein according to claim 2, characterized in that: The sampling assembly (105) also includes a rubber block (105c) movably connected to the inner cavity of the sampling port (105b) and a retaining ring (105d) sleeved on the side wall of the sampling port (105b).

4. The purification apparatus for an active protein according to claim 3, characterized in that: The connecting assembly (106) includes a pressing component (106a) and an auxiliary component (106b) disposed below the pressing component (106a) for use in conjunction with the pressing component (106a).

5. The purification apparatus for an active protein according to claim 4, characterized in that: The pressing component (106a) includes a pressing base (106a-1), an auxiliary groove (106a-2) formed at the bottom of the pressing base (106a-1), and a washer (106a-3) fixedly installed at the bottom of the pressing base (106a-1).

6. The purification apparatus for an active protein according to claim 5, characterized in that: The pressing component (106a) also includes a limiting groove (106a-4) formed on the side wall of the pressing base (106a-1), and a filter screen (106a-5) movably connected to the side wall of the gasket (106a-3).

7. The purification apparatus for an active protein according to claim 6, characterized in that: The auxiliary component (106b) includes a top seat (106b-1), a tension spring (106b-2) fixedly connected to the inner cavity of the top seat (106b-1), a top block (106b-3) movably connected to the side wall of the top seat (106b-1), and a knob (106b-4) fixedly installed on the side wall of the top block (106b-3).

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