Enzymolysis device for polypeptide production

Abstract

The utility model relates to the technical field of enzymolysis equipment, in particular to an enzymolysis device for polypeptide production, which comprises a reaction tank and an enzymolysis mixing component, the enzymolysis mixing assembly comprises a stirring shaft, an enzymatic hydrolysate box, an electric push rod, a spring, a base and a stirring rod, the stirring shaft is rotationally connected with the reaction tank, the stirring shaft penetrates through the reaction tank, the enzymatic hydrolysate box is slidably connected with the stirring shaft, the enzymatic hydrolysate box is located on the outer side of the stirring shaft, the base is rotationally connected with the reaction tank, and the base is located at the bottom of the inner side of the reaction tank; the upper end and the lower end of the spring are fixedly connected with the enzymatic hydrolysate box and the base respectively, the stirring shaft is slidably connected with the base, a plurality of liquid outlets are formed in the peripheral side of the enzymatic hydrolysate box at intervals, a plurality of stirring rods are arranged on the peripheral side of the enzymatic hydrolysate box at intervals, and the electric push rod is fixedly installed on the reaction tank and located above the enzymatic hydrolysate box; the stirring rod moves up and down while rotating, so that the mixing effect of polypeptide and enzymatic hydrolysate is improved, the mixing uniformity is improved, and the enzymolysis efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of enzymatic hydrolysis equipment technology, and in particular to an enzymatic hydrolysis device for polypeptide production. Background Technology

[0002] Existing enzymatic hydrolysis devices generally have a stirring assembly inside, which includes a stirring motor located outside the enzymatic hydrolysis device and stirring blades located inside the enzymatic hydrolysis device. However, existing enzymatic hydrolysis devices have poor uniformity of stirring effect during the enzymatic hydrolysis process, and local temperature rise is easily caused around the stirring blades, which affects the enzymatic hydrolysis yield.

[0003] The utility model patent with publication number CN207362212U provides an enzymatic hydrolysis device, including a tank and a stirring device. The stirring device includes a stirring motor mounted on the tank, a stirring shaft arranged vertically inside the tank, and stirring blades mounted on the stirring shaft. A circulating water tank is provided outside the tank, and the circulating water tank is provided with a return water port and a water outlet. A cooler and a water pump connected to the water outlet are provided inside the circulating water tank. The stirring motor is a hollow motor with a stirring shaft passing through the stirring motor body. The upper part of the stirring shaft is provided with a return water connection sleeve and a water outlet connection sleeve. A return water channel and a water outlet channel are opened inside the stirring shaft. The stirring blade is a spiral circulating blade, and the spiral circulating blade is provided with a water outlet stirring channel communicating with the water outlet channel and a return water stirring channel communicating with the return water channel.

[0004] However, the above-mentioned enzymatic hydrolysate was added together with the enzymatic sample, resulting in uneven mixing and reduced enzymatic hydrolysis efficiency. Utility Model Content

[0005] The purpose of this invention is to provide an enzymatic hydrolysis device for peptide production, which solves the problem that the enzymatic hydrolysate is added together with the enzymatic sample, resulting in uneven mixing and reduced enzymatic hydrolysis efficiency.

[0006] To achieve the above objectives, this utility model provides an enzymatic hydrolysis device for peptide production, comprising a reaction vessel and an enzymatic hydrolysis mixing assembly. The enzymatic hydrolysis mixing assembly includes a stirring shaft, an enzymatic hydrolysate tank, an electric push rod, a spring, a base, and stirring rods. The stirring shaft is rotatably connected to the reaction vessel and passes through the reaction vessel. The enzymatic hydrolysate tank is slidably connected to the stirring shaft and is located outside the stirring shaft. The base is rotatably connected to the reaction vessel and is located at the bottom inner side of the reaction vessel. The upper and lower ends of the spring are fixedly connected to the enzymatic hydrolysate tank and the base, respectively. The stirring shaft is slidably connected to the base. The enzymatic hydrolysate tank has multiple outlets spaced apart on its periphery. The enzymatic hydrolysate tank has multiple stirring rods spaced apart on its periphery. The electric push rod is fixedly installed on the reaction vessel and located above the enzymatic hydrolysate tank.

[0007] The enzymolysis mixing assembly further comprises a rotating ring, the rotating ring is rotationally connected with the enzymolysis liquid tank, and the rotating ring is located below the electric push rod.

[0008] The enzymolysis mixing assembly further comprises a mounting seat and a ball, the mounting seat is fixedly connected with the output end of the electric push rod, the bottom of the mounting seat is provided with a spherical groove, and the ball is movably arranged in the spherical groove.

[0009] The enzymolysis mixing assembly further comprises a protection structure, the protection structure comprises an outer baffle and an inner baffle, the bottom of the outer baffle is fixedly connected with the base, the top of the inner baffle is fixedly connected with the enzymolysis liquid tank, and the inner baffle is slidably connected with the outer baffle.

[0010] The outer side of the inner baffle is provided with a limiting support, the inner side of the outer baffle is provided with a limiting groove matched with the limiting support, and the limiting support is located in the limiting groove.

[0011] The polypeptide production enzymolysis device, when the stirring shaft rotates, the enzymolysis liquid tank rotates under the driving of the stirring shaft, so that the enzymolysis liquid in the enzymolysis liquid tank is thrown out, and the stirring rod stirs the polypeptide and the enzymolysis liquid; meanwhile, the electric push rod operates to extrude the enzymolysis liquid tank downward, so that the enzymolysis liquid tank moves downward, then the electric push rod resets, and the enzymolysis liquid tank moves upward under the action of the spring, so as to repeatedly move up and down, so that the stirring rod moves up and down while rotating, thereby improving the mixing effect of the polypeptide and the enzymolysis liquid, improving the mixing uniformity, improving the enzymolysis efficiency, and solving the problem that the enzymolysis liquid is put together with the enzymolysis sample, resulting in uneven mixing and reducing the enzymolysis efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0012] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced.

[0013] Fig. 1 is a structural schematic view of the polypeptide production enzymolysis device.

[0014] Fig. 2 is a structural sectional view of the polypeptide production enzymolysis device.

[0015] Fig. 3 is a structural sectional view of the inner part of the outer baffle of the polypeptide production enzymolysis device.

[0016] 100-reaction tank, 210-stirring shaft, 220-enzymatic hydrolysis liquid tank, 221-liquid outlet, 230-electric push rod, 240-spring, 250-base, 260-stirring rod, 270-rotary ring, 280-mounting seat, 281-spherical groove, 290-rolling ball, 310-outer baffle, 311-limiting groove, 320-inner baffle, 321-limiting support. DETAILED DESCRIPTION

[0017] The embodiments of the present application are described in detail below, examples of which are shown in the drawings. The embodiments described below by referring to the drawings are exemplary and are intended to explain the present application, and cannot be understood as a limitation of the present application.

[0018] Please refer to Figs. 1 to 3 , Fig. 1 is a structural schematic view of the enzymatic hydrolysis device for polypeptide production, Fig. 2 is a structural sectional view of the enzymatic hydrolysis device for polypeptide production, Fig. 3 is a structural sectional view of the inner part of the outer baffle of the enzymatic hydrolysis device for polypeptide production.

[0019] The present application provides an enzymatic hydrolysis device for polypeptide production, comprising a reaction tank 100 and an enzymatic hydrolysis mixing assembly, the enzymatic hydrolysis mixing assembly comprising a stirring shaft 210, an enzymatic hydrolysis liquid tank 220, an electric push rod 230, a spring 240, a base 250, a stirring rod 260, a rotary ring 270, a mounting seat 280, a rolling ball 290 and a protective structure, the protective structure comprising an outer baffle 310 and an inner baffle 320.

[0020] For this specific embodiment, the stirring shaft 210 is rotationally connected with the reaction tank 100, the stirring shaft 210 is arranged in the reaction tank 100, the enzymatic hydrolysis tank 220 is slidingly connected with the stirring shaft 210, the enzymatic hydrolysis tank 220 is located outside the stirring shaft 210, the base 250 is rotationally connected with the reaction tank 100, the base 250 is located at the inner bottom of the reaction tank 100, the upper and lower ends of the spring 240 are fixedly connected with the enzymatic hydrolysis tank 220 and the base 250 respectively, the stirring shaft 210 is slidingly connected with the base 250, the periphery of the enzymatic hydrolysis tank 220 is spaced apart to be provided with a plurality of liquid outlets 221, the periphery of the enzymatic hydrolysis tank 220 is spaced apart to be provided with a plurality of stirring rods 260, and the electric push rod 230 is fixedly installed on the reaction tank 100 and located above the enzymatic hydrolysis tank 220. The enzymatic hydrolysis tank 220 is used to place the enzymatic hydrolysis liquid, the electric push rod 230 can push the enzymatic hydrolysis tank 220 downward, the lower end of the stirring shaft 210 is square in cross section, the stirring shaft 210 is slidingly connected with the base 250 through the enzymatic hydrolysis tank 220, the spring 240 upwardly abuts against the enzymatic hydrolysis tank 220, when the electric push rod 230 extrudes the enzymatic hydrolysis tank 220 downward to make the enzymatic hydrolysis tank 220 move downward, the spring 240 is compressed to have a restoring force, so that the enzymatic hydrolysis tank 220 can be reset under the action of the spring 240, so that the enzymatic hydrolysis tank 220 can reciprocate upwardly and downwardly under the actions of the electric push rod 230 and the spring 240. The stirring shaft 210 can rotate under the action of the motor, when the stirring shaft 210 rotates, the enzymatic hydrolysis tank 220 rotates under the driving of the stirring shaft 210, so as to throw out the enzymatic hydrolysis liquid in the enzymatic hydrolysis tank 220, at the same time, the stirring rods 260 stir the polypeptide and the enzymatic hydrolysis liquid, at the same time of stirring, the electric push rod 230 operates to extrude the enzymatic hydrolysis tank 220 downward to make the enzymatic hydrolysis tank 220 move downward, then the electric push rod 230 resets, the enzymatic hydrolysis tank 220 moves upward under the action of the spring 240, and the operations are repeated, so that the enzymatic hydrolysis tank 220 reciprocates upwardly and downwardly while rotating, and the stirring rods 260 move upwardly and downwardly while rotating, so as to improve the mixing effect of the polypeptide and the enzymatic hydrolysis liquid, improve the mixing uniformity, improve the enzymatic hydrolysis efficiency, and solve the problem that the enzymatic hydrolysis liquid is placed together with the enzymatic hydrolysis sample to cause uneven mixing and reduce the enzymatic hydrolysis efficiency.

[0021] Further, the rotating ring 270 is rotationally connected with the enzymatic hydrolysis tank 220, and the rotating ring 270 is located below the electric push rod 230. The rotating ring 270 can rotate relative to the enzymatic hydrolysis tank 220, so that the electric push rod 230 extrudes the rotating ring 270 when the electric push rod 230 extrudes the enzymatic hydrolysis tank 220 downward, and the electric push rod 230 extruding the rotating ring 270 will not affect the rotation of the enzymatic hydrolysis tank 220.

[0022] Specifically, the mounting seat 280 is fixedly connected with the output end of the electric push rod 230, and the bottom of the mounting seat 280 is provided with a spherical groove 281, and the ball 290 is movably arranged in the spherical groove 281. The mounting seat 280 is used for mounting the ball 290, and the ball 290 can rotate in the spherical groove 281. When the electric push rod 230 extrudes the enzymatic hydrolysis tank 220 downward, the ball 290 contacts the rotating ring 270, and the ball 290 can roll to reduce friction.

[0023] The bottom of the outer baffle 310 is fixedly connected with the base 250, the top of the inner baffle 320 is fixedly connected with the enzymatic hydrolysis tank 220, and the inner baffle 320 is slidably connected with the outer baffle 310. The protection structure is used for protecting the spring 240, and the inner baffle 320 and the outer baffle 310 are arranged outside the spring 240. The inner baffle 320 can slide up and down relative to the outer baffle 310, so that the enzymatic hydrolysis tank 220 can move up and down without being affected.

[0024] In addition, the outer side of the inner baffle 320 is provided with a limiting support 321, the inner side of the outer baffle 310 is provided with a limiting groove 311 matched with the limiting support 321, and the limiting support 321 is located in the limiting groove 311. The limiting support 321 can slide in the limiting groove 311, and the limiting support 321 and the limiting groove 311 cooperate to achieve the sliding connection between the inner baffle 320 and the outer baffle 310.

[0025] When the polypeptide is mixed by using the enzymatic hydrolysis device for polypeptide production, the polypeptide is first placed in the reaction tank 100, and then the enzymatic hydrolysis solution is placed in the enzymatic hydrolysis solution tank 220. The stirring shaft 210 rotates under the drive of the motor, and the enzymatic hydrolysis solution tank 220 rotates under the drive of the stirring shaft 210, so as to throw out the enzymatic hydrolysis solution inside the enzymatic hydrolysis solution tank 220. At the same time, the stirring rod 260 stirs the polypeptide and the enzymatic hydrolysis solution. At the same time of stirring, the electric push rod 230 operates to press downward the enzymatic hydrolysis solution tank 220, so as to make the enzymatic hydrolysis solution tank 220 move downward. Then, the electric push rod 230 resets, and the enzymatic hydrolysis solution tank 220 moves upward under the action of the spring 240. In this way, the enzymatic hydrolysis solution tank 220 rotates while moving up and down repeatedly, and the stirring rod 260 rotates while moving up and down, so as to improve the mixing effect of the polypeptide and the enzymatic hydrolysis solution, improve the mixing uniformity, and improve the enzymatic hydrolysis efficiency.

[0026] The above disclosure is only one or more preferred embodiments of the present application, and cannot limit the scope of the rights of the present application. Those skilled in the art can understand that all or part of the above-mentioned embodiments can be implemented, and equivalent changes made according to the claims of the present application still belong to the scope covered by the present application.

Claims

1. An enzymatic hydrolysis device for polypeptide production, comprising a reaction tank, characterized in that, it further comprises an enzymatic hydrolysis mixing assembly; the enzymatic hydrolysis mixing assembly comprises a stirring shaft, an enzymatic hydrolysis liquid tank, an electric push rod, a spring, a base and a stirring rod, the stirring shaft is rotationally connected with the reaction tank, the stirring shaft is arranged in the reaction tank, the enzymatic hydrolysis liquid tank is slidingly connected with the stirring shaft, the enzymatic hydrolysis liquid tank is located outside the stirring shaft, the base is rotationally connected with the reaction tank, the base is located at the inner bottom of the reaction tank, the upper and lower ends of the spring are fixedly connected with the enzymatic hydrolysis liquid tank and the base respectively, the stirring shaft is slidingly connected with the base, a plurality of liquid outlets are arranged at intervals on the circumferential side of the enzymatic hydrolysis liquid tank, a plurality of stirring rods are arranged at intervals on the circumferential side of the enzymatic hydrolysis liquid tank, and the electric push rod is fixedly installed on the reaction tank and located above the enzymatic hydrolysis liquid tank.

2. The enzymatic hydrolysis device for polypeptide production according to claim 1, characterized in that, the enzymatic hydrolysis mixing assembly further comprises a swivel, the swivel is rotationally connected with the enzymatic hydrolysis liquid tank, and the swivel is located below the electric push rod.

3. The enzymatic hydrolysis device for polypeptide production according to claim 2, characterized in that, the enzymatic hydrolysis mixing assembly further comprises a mounting seat and a ball, the mounting seat is fixedly connected with the output end of the electric push rod, the bottom of the mounting seat has a spherical groove, and the ball is movably arranged in the spherical groove.

4. The enzymatic hydrolysis device for polypeptide production according to claim 1, characterized in that, the enzymatic hydrolysis mixing assembly further comprises a protection structure, the protection structure comprises an outer baffle and an inner baffle, the bottom of the outer baffle is fixedly connected with the base, the top of the inner baffle is fixedly connected with the enzymatic hydrolysis liquid tank, and the inner baffle is slidingly connected with the outer baffle.

5. The enzymatic hydrolysis device for polypeptide production according to claim 4, characterized in that, a limiting support is arranged on the outer side of the inner baffle, the inner side of the outer baffle has a limiting groove matched with the limiting support, and the limiting support is located in the limiting groove.

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