Spacer and antibody incubation box for WB experiment containing same
By using spacers to separate the PVDF membranes in the antibody incubation chamber, the problem of incomplete antibody incubation caused by membrane overlap was solved, improving the accuracy of WB experiments and reducing costs.
Patent Information
- Application Number
- CN202422866746.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-23
- Publication Date
- 2025-11-28
- Estimated Expiration
- 2034-11-23
AI Technical Summary
In Western blot (WB) experiments, multiple PVDF membranes can easily overlap during antibody incubation, leading to incomplete antibody incubation or incomplete washing, which affects the accuracy and reliability of the experimental results.
A spacer, including a slot, a plate, and separating teeth, is designed to separate PVDF membranes and set an incubation chamber in the antibody incubation box to ensure that the flow of antibody working solution is unimpeded and to achieve effective separation between membranes.
This effectively avoids membrane overlap, ensures sufficient contact between antibodies and proteins, improves experimental accuracy, reduces antibody working solution waste, and lowers experimental costs.
Smart Images
Figure CN223611511U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the technical field of antibody incubation box, concretely speaking is the spacer and the WB experiment with antibody incubation box of containing the spacer. BACKGROUND
[0002] In the WB (Western Blotting, Western Blot) experiment, the antibody incubation box is a key experimental tool, which is used to incubate the antibody in a specific temperature and time, to ensure that the antibody is specifically combined with the protein on the membrane, in the process of antibody and PVDF membrane protein binding, it needs to be shaken in a low temperature environment for a period of time on the shaking table, and in the process of secondary antibody incubation, if fluorescent secondary antibody is used, there is the need of light shielding, and the light shielding antibody incubation box is needed.
[0003] At present, the light shielding antibody incubation box for WB experiment is mainly black single grid, after cutting, the PVDF membrane is placed in the light shielding antibody incubation box, and then the antibody working solution is added, under the action of the flowing of the antibody working solution and the shaking table, the multiple PVDF membranes will overlap, resulting in incomplete antibody incubation or incomplete cleaning, and then affecting the accuracy of the WB experiment results. UTILITY MODEL CONTENT
[0004] In order to solve the above technical problems, the utility model provides the spacer and the WB experiment with antibody incubation box containing the spacer, to solve the problem that in the process of incubating the antibody, multiple PVDF membranes overlap under the action of the flowing of the antibody working solution and the shaking table, affecting the incomplete antibody incubation or incomplete cleaning.
[0005] The spacer comprises:
[0006] The spacer body comprises a plurality of clamping grooves and at least two clamping plates, the two clamping plates are clamped with the clamping grooves, a connecting plate is installed between the two clamping plates, and a plurality of separation teeth are uniformly connected to the lower end of the connecting plate.
[0007] Preferably, a gap is provided between adjacent separation teeth.
[0008] The WB experiment with antibody incubation box containing the spacer comprises:
[0009] The box body, a plurality of clamping grooves are opened in the four side surfaces inside the box body, the box body is provided with an incubation cavity, the antibody working solution is placed in the incubation cavity, the spacer body is arranged in the inside of the box body, and the spacer body is provided with at least one, which divides the incubation cavity into at least two incubation spaces.
[0010] The PVDF membranes are arranged inside the incubation cavity and float on the antibody working solution, the spacer body separates at least two PVDF membranes, and the antibody working solution flows between the separation teeth.
[0011] Preferably, the bottom wall of the clamping groove is above the bottom wall of the incubation cavity.
[0012] Preferably, the total length of the spacer body comprises a longitudinal separation length and a transverse separation length.
[0013] Preferably, the upper end of the box body is provided with a sealing cover.
[0014] Preferably, the box body and the sealing cover are connected through a connecting buckle.
[0015] Compared with the prior art, the utility model has the beneficial effects that:
[0016] 1、The utility model discloses a separation tooth separates the PVDF membrane on both sides, thereby can effectively separate the PVDF membrane on both sides, avoids the overlapping interference experiment result between the membrane, reduces the accuracy and reliability of WB experiment, and the clearance between the separation tooth allows the antibody working solution to flow freely, and will not be hindered because of the existence of the spacer body, so that the antibody working solution can smoothly pass through each separation area of the incubation cavity and fully contacts the protein on the PVDF membrane, guarantees the smooth progress of the experiment and the accuracy of the result.
[0017] 2、The depth of the clamping groove is lower than the depth of the incubation cavity, the bottom end of the clamping groove is flush with the liquid level of the antibody working solution with the maximum volume in the incubation cavity, thereby can reduce the antibody working solution into the clamping groove, causes the waste of the antibody working solution, reduces the cost of WB experiment. DETAILED DESCRIPTION
[0018] Figure 1 It is the antibody incubation box structure schematic view for the utility model's WB experiment;
[0019] Figure 2 It is the first visual angle structure schematic view for the utility model;
[0020] Figure 3 It is the spacer body partial structure schematic view for the utility model;
[0021] Figure 4 It is the second visual angle structure schematic view for the utility model;
[0022] Figure 5 It is the third visual angle structure schematic view for the utility model.
[0023] In the drawing:
[0024] 1, spacer body; 2, card slot; 3, card plate; 4, connecting plate; 5, separation tooth; 6, box body; 61, incubation cavity; 7, sealing cover; 8, connecting buckle; 9, PVDF membrane. DETAILED DESCRIPTION
[0025] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. It should be pointed out that the drawings are schematic and not drawn to scale. For the sake of clarity and convenience in the drawings, the relative size and proportion of the parts shown in the drawings are exaggerated or reduced for illustration, and any size is only exemplary, rather than limiting.
[0026] Embodiment one
[0027] Reference Figures 1-3 The utility model provides a spacer and antibody incubation box for WB experiment containing the spacer, comprising:
[0028] The box body 6 is provided with an incubation cavity 61, and the antibody working solution is placed in the incubation cavity 61. The box body 6 is made of black material, and the antibody working solution in the incubation cavity 61 and the PVDF membrane 9 are protected from light;
[0029] The PVDF membrane 9 is arranged in the incubation cavity 61 and floats on the antibody working solution. The antibody working solution in the incubation cavity 61 is combined with the protein on the PVDF membrane 9, and the WB experiment is completed;
[0030] The spacer body 1 is arranged in the box body 6, and the spacer body 1 is provided with at least one, which divides the incubation cavity 61 into at least two incubation spaces. The spacer body 1 isolates at least two PVDF membranes 9. The spacer body 1 comprises a plurality of card slots 2 and at least two card plates 3. The plurality of card slots 2 are arranged on the four sides of the box body 6. The two card plates 3 are connected with the card slots 2. The connecting plate 4 is arranged between the two card plates 3. The connecting plate 4 is uniformly connected with a plurality of separation teeth 5 at the lower end. The plurality of separation teeth 5 are provided with gaps between adjacent separation teeth 5. The plurality of separation teeth 5 separate the two PVDF membranes 9, and the antibody working solution flows in the gaps between the plurality of separation teeth 5. The plurality of PVDF membranes 9 can be prevented from overlapping together under the action of buoyancy and shaking in the incubation process.
[0031] Specifically, the user determines the number and size of the incubation spaces required for separating the incubation cavity 61 according to the requirements of the WB experiment, thereby determining the placement position and number of the spacer body 1, taking out the spacer body 1, placing the separation teeth 5 inside the incubation cavity 61, clamping the clamping plate 3 in the corresponding clamping groove 2 to limit the spacer body 1, and separating the PVDF membranes 9 on both sides by the separation teeth 5, so as to effectively separate the PVDF membranes 9 on both sides, avoid the overlapping of the membranes, reduce the accuracy and reliability of the WB experiment, and improve the space utilization of the incubation cavity 61 in the box body 6. Meanwhile, the gap between the separation teeth 5 allows the antibody working solution to flow freely and is not hindered by the spacer body 1. In this way, the antibody working solution can smoothly pass through each separated area of the incubation cavity 61 and fully contact the proteins on the PVDF membrane 9, thereby ensuring the smooth progress of the experiment and the accuracy of the results.
[0032] Embodiment Two
[0033] With reference to Figures 4-5 The utility model provides a spacer and WB experiment antibody incubation box containing the spacer, including clamping groove 2, the bottom wall of clamping groove 2 is located the bottom wall of incubation cavity 61 above, the bottom end of clamping groove 2 and incubation cavity 61 install the antibody working solution liquid level of maximum volume flush, thereby can reduce the antibody working solution to enter the inside of clamping groove 2, cause the waste of antibody working solution, reduce the cost of WB experiment.
[0034] The total length of the spacer body 1 includes the longitudinal separation length and the transverse separation length of the incubation cavity 61.
[0035] Specifically, multiple spacer bodies 1 required for longitudinal separation and multiple spacer bodies 1 required for transverse separation of the incubation cavity 61 can be arranged, so that the user can choose to longitudinally separate or transversely separate the incubation space of the incubation cavity 61 according to requirements when the user needs to separate the incubation space of the incubation cavity 61, thereby improving the flexibility of the device.
[0036] Embodiment Three
[0037] With reference to Figure 4 The utility model provides a spacer and WB experiment antibody incubation box containing the spacer, including the box body 6 upper end is provided with sealing cover 7, sealing cover 7 adopts black material to make, and the inside experiment material of box body 6 is photophobic.
[0038] The box body 6 and the sealing cover 7 are connected through the connecting fastener 8, and the connecting fastener 8 includes, but is not limited to, a round buckle, a plug buckle, a belt buckle, an elastic cantilever clamp and a cylindrical clamping piece.
[0039] Specific implementation: the box body 6 can be sealed by sealing the cover 7 and locking the cover 7 by using the connecting buckle 8.
[0040] The standard parts used in the utility model can be purchased from the market, and the special-shaped parts can be ordered according to the description and the drawings, the specific connection mode of each part adopts the conventional means such as bolts, rivets and welding in the prior art, the mechanical parts and equipment adopt conventional models in the prior art, and the circuit connection adopts the conventional connection mode in the prior art, which will not be described in detail herein. The contents not described in detail in the description belong to the prior art known to those skilled in the art.
[0041] In the description of the utility model, the terms "first" and "second" are only used for the purpose of description, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first" and "second" can explicitly or implicitly include one or more of the features. The meaning of "a plurality of" is two or more, unless otherwise specifically limited.
[0042] In the utility model, unless otherwise specifically defined and limited, the terms "mounting", "connection", "connection", "fixing" and the like should be understood in a broad sense, for example, it can be fixed connection, or detachable connection, or integrated; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through intermediate medium, or the communication between two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.
[0043] In the utility model, unless otherwise specifically defined and limited, the first feature is "on" or "under" the second feature, which can be direct contact between the first and second features, or indirect contact between the first and second features through intermediate medium. Moreover, the first feature "above", "above" and "above" the second feature can be directly above or obliquely above the first feature, or only indicate that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "below" and "below" the second feature can be directly below or obliquely below the first feature, or only indicate that the horizontal height of the first feature is less than that of the second feature.
[0044] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "an example", "a specific example" or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. Furthermore, the person skilled in the art can combine and combine the different embodiments or examples described in the present specification and the features of the different embodiments or examples, without contradiction.
[0045] The present application discloses embodiments in the drawings, only relate to the structure involved in the present application, other structures can refer to the usual design, in the case of no conflict, the same embodiment and different embodiments of the present application can be combined with each other.
[0046] Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacements to some of the technical features, and any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.
Claims
1. A spacer, characterized by: Include: The spacer body (1) comprises several clamping grooves (2) and is provided with at least two clamping plates (3), two clamping plates (3) are clamped with clamping grooves (2), and connecting plates (4) are installed between two clamping plates (3), and the lower end of the connecting plate (4) is uniformly connected with several separation teeth (5).
2. The spacer of claim 1, wherein: Several adjacent separation teeth (5) are provided with gaps.
3. Antibody incubation box for WB experiments comprising a spacer according to any one of claims 1-2, characterized in that: Include: The box body (6) is provided with an incubation cavity (61) inside the four sides of the box body (6), and the antibody working solution is placed inside the incubation cavity (61), the spacer body (1) is arranged inside the box body (6), and the spacer body (1) is provided with at least one, which divides the incubation cavity (61) into at least two incubation spaces; PVDF membrane (9) is arranged inside the incubation cavity (61) and floats on the antibody working solution, the spacer body (1) isolates at least two PVDF membranes (9), and several separation teeth (5) separate the two PVDF membranes (9) and the antibody working solution flows in the gaps of the several separation teeth (5).
4. The antibody incubation box for WB experiments of claim 3, wherein: The bottom wall of the clamping groove (2) is above the bottom wall of the incubation cavity (61).
5. The antibody incubation box for WB experiments of claim 3, wherein: The total length of the spacer body (1) includes the longitudinal separation length and the transverse separation length of the incubation cavity (61).
6. The antibody incubation box for WB experiments of claim 3, wherein: The upper end of the box body (6) is provided with a sealing cover (7).
7. The antibody incubation box for WB experiments of claim 6, wherein: The box body (6) and the sealing cover (7) are connected through the connecting buckle (8).