Electronic fluorescence test paper disease detection pen type sensor

Abstract

The utility model discloses an electronic fluorescence test paper disease detection pen type sensor, which adopts a dichroscope, a purple optical filter and a red optical filter to form an optical path, and reduces the use of a lens group compared with an existing fluorescence immunochromatography instant detector. A control circuit module and a photoelectric detection module are integrated in the electronic fluorescence test paper disease detection pen type sensor, and the sensor has the advantages of being small in size, compact in structure and convenient to carry.

Description

TECHNICAL FIELD

[0001] The utility model belongs to fluorescent detection technical field, concretely relates to a kind of electronic fluorescent test paper disease detection pen type sensor. BACKGROUND

[0002] In the field of rapid diagnosis, the use of fluorescent test paper disease detection products is increasingly common. Fluorescent immunoassay is a method that uses fluorescently labeled antibodies or antigens to specifically bind to target substances in a sample, and then moves the bound complex along a specific path on the test strip by immunochromatography. In the specific detection area, the fluorescently labeled complex is captured and produces a fluorescent signal, and the intensity of the fluorescent signal is detected to determine the content of the target substance in the sample. This method has high sensitivity, strong specificity and can achieve quantitative detection. However, this method requires the use of fluorescence analysis or portable UV flashlight, which restricts the use of the product in the market and makes it inconvenient to use in a home environment, which delays the discovery and treatment of the disease to some extent. SUMMARY

[0003] The utility model aims at providing a kind of electronic fluorescent test paper disease detection pen type sensor, filter and dichroic mirror are integrated in a smaller shell by forming optical path, realize the miniaturization of fluorescent test paper disease detection product.

[0004] The technical scheme of the utility model is as follows:

[0005] A kind of electronic fluorescent test paper disease detection pen type sensor is used to detect fluorescent test paper, and the fluorescent test paper has detection line and reference line, detection line and reference line are arranged on the same side of fluorescent test paper with interval, and the electronic fluorescent test paper disease detection pen type sensor includes shell, control circuit module, display screen and photoelectric detection module;

[0006] The photoelectric detection module includes a housing, a light source, a purple filter, a dichroic mirror, a red filter and a photodiode.

[0007] The housing is provided with a first passage through in a first direction and a second passage perpendicular to the first passage, one end of the second passage is located on the surface of the housing, and the other end of the second passage communicates with the first passage.

[0008] The dichroic mirror is arranged at the intersection of the first passage and the second passage, and the included angle between the dichroic mirror and the first passage and the second passage is 45°, the first passage is provided with a purple filter and a light source outward from the dichroic mirror in sequence, and the second passage is provided with a red filter and a photodiode outward from the dichroic mirror in sequence.

[0009] The shell has opposite first and second ends, a receiving cavity is formed in the first end, a slide rail is formed in the side wall of the second end along the extension direction of the shell, a test paper insertion port is formed in the end face of the second end and is parallel to the slide rail, a slide cavity is formed in the second end and is in communication with the receiving cavity, the slide rail and the test paper insertion port;

[0010] The control circuit module is accommodated in the receiving cavity, the display screen is arranged on the outer wall of the shell, the photoelectric detection module is slidably connected with the slide cavity, the first direction is the extension direction of the slide rail, and the red filter is located between the photodiode and the slide cavity;

[0011] The fluorescent test paper enters the slide cavity through the test paper insertion port, and the photoelectric detection module is slid along the slide cavity, so that the first lens can be aligned with the detection line and the reference line of the fluorescent test paper with different lengths;

[0012] The control circuit module is connected with the display screen, the photodiode and the light source in control and power supply.

[0013] In a preferred embodiment, the electronic fluorescent test paper disease detection pen-type sensor further comprises a first switch sensing module and a second switch sensing module, and the first switch sensing module and the second switch sensing module are arranged in sequence on the inner wall of the slide cavity from the direction of the test paper insertion port to the receiving cavity;

[0014] The first switch sensing module and the second switch sensing module are connected with the control circuit module in control and are powered by the control circuit module;

[0015] When the fluorescent test paper enters the slide cavity through the test paper insertion port and contacts the first switch sensing module or the second switch sensing module, the first switch sensing module or the second switch sensing module sends a signal to the control circuit module and controls the light source to be turned on by the control circuit module.

[0016] In a preferred embodiment, the emission wavelength of the light source is 365 nm, the purple filter is a 365 nm band-pass filter, the dichroic mirror is a dichroic beam splitter that reflects 365 nm light fibers and transmits 615 nm light fibers, and the red filter is a 615 nm band-pass filter.

[0017] In a preferred embodiment, the control circuit module comprises a power supply, a main control chip and an ADC conversion chip;

[0018] The ADC conversion chip is used to receive the electrical signal emitted by the photodiode, then convert it into a digital signal and transmit it to the main control chip;

[0019] The main control chip is connected with the first switch sensing module, the second switch sensing module, the light source, the display screen and the ADC conversion chip in control;

[0020] The power supply supplies power for the first switch induction module, the second switch induction module, the light source, the display screen, the ADC conversion chip and the main control chip.

[0021] Further preferably, the main control chip is an STM32F103C8T6 chip, the ADC conversion chip is an AD7686BRMZ chip, and the power supply is a rechargeable lithium battery.

[0022] In a preferred embodiment, two positioning columns are arranged in the accommodating cavity, the control circuit module is fixedly connected to the accommodating cavity through the positioning columns, and the control circuit is not in contact with the side wall of the accommodating cavity.

[0023] In a preferred embodiment, the shell comprises an upper shell and a lower shell which are detachably connected.

[0024] The accommodating cavity is formed in the upper shell, and the slide rail, the slide cavity and the test paper insertion port are formed on the lower shell.

[0025] Further preferably, the upper shell is further provided with a first connecting sliding groove which is in communication with the accommodating cavity and the bottom surface of the upper shell.

[0026] The lower shell is further provided with a second connecting sliding groove which is in communication with the slide cavity and the top surface of the lower shell.

[0027] When the upper shell is connected to the lower shell, the first connecting sliding groove and the second connecting sliding groove are in communication.

[0028] Further preferably, the slide rail is provided with a limiting block at one end close to the second connecting sliding groove.

[0029] When the photoelectric detection module moves to the top end of the slide rail, the limiting block is in abutment with the photoelectric detection module.

[0030] In a preferred embodiment, the lower end of the slide rail is further provided with a positioning block.

[0031] When the fluorescent test paper is inserted into the test paper insertion port and the shell is in abutment with the positioning block, the reference line is aligned with the second channel.

[0032] The utility model has at least the following beneficial effects:

[0033] (1) The light path is composed of a dichroic mirror, a purple filter and a red filter, the use of lens groups is reduced compared with the existing fluorescent immunochromatographic instant detector, and the control circuit module and the photoelectric detection module are integrated in the electronic fluorescent test paper disease detection pen sensor, so that the device has the advantages of small size, compact structure and convenient carrying.

[0034] (2) Through the cooperation of the photoelectric detection module and the slide rail, the photoelectric detection module can move up and down to align the light path with the reference line or the detection line of the fluorescent test paper, so that the adaptation to most fluorescent test papers on the market is realized, and the user can use conveniently. Attached Figure Description

[0035] Figure 1 This is a schematic diagram of the electronic fluorescent test strip disease detection pen sensor and fluorescent test strip provided in Example 1;

[0036] Figure 2 A longitudinal cross-sectional view of the electronic fluorescent test strip disease detection pen sensor provided in Example 1;

[0037] Figure 3 A cross-sectional view of the photoelectric detection module provided in Example 1;

[0038] Figure 4 The circuit structure diagram of the main control chip provided in Example 1;

[0039] Figure 5 The circuit structure diagram of the ADC conversion chip provided in Example 1 is shown.

[0040] The reference numerals in the figure are as follows: 1-Upper outer shell; 11-Display screen; 12-Control circuit module; 13-First connecting slide; 14-Right side positioning post; 15-Left side positioning post; 16-Accommodation cavity; 2-Lower outer shell; 21-Slide rail; 22-Photoelectric detection module; 23-Limiting block; 24-Positioning block; 25-Slide cavity; 26-Second connecting slide; 251-First switch sensing module; 252-Second switch sensing module; 253-Reagent port; 221-Shell; 222-Light source; 223-Purple filter; 224-Dichroic mirror; 225-Red filter; 226-Photodiode; 3-Fluorescent test paper; 31-Reference line; 32-Detection line. Detailed Implementation

[0041] The technical solution of this utility model will be further explained and described below through specific embodiments. To facilitate understanding of the embodiments of this utility model, further explanation and description will be provided below with reference to the accompanying drawings and specific examples. These embodiments do not constitute a limitation on the embodiments of this utility model.

[0042] The terms "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer" used in this document to indicate orientation or positional relationships are based on the orientation or positional relationships shown in the accompanying drawings. They are used solely for the convenience of describing this utility model and for simplifying the description, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0043] Unless otherwise defined, the terms "mounting", "connected", "connecting" should be interpreted broadly, for example, can be fixedly connected, can also be detachably connected, or integrally connected; can be mechanically connected, can also be electrically connected; can be directly connected, can also be indirectly connected through an intermediate medium. The specific meaning of the above terms in the utility model can be understood by the person skilled in the art according to the specific circumstances. The "control connection" in the following embodiments is connected through a wire, and when each component is "control connected" with the control circuit module, it transmits signals to the control circuit module or opens or closes according to the instruction of the control circuit module.

[0044] Embodiment 1

[0045] As shown in Figure 1 and Figure 2 The embodiment provides a kind of electronic fluorescent test paper disease detection pen sensor for detecting fluorescent test paper 3, and fluorescent test paper 3 has detection line 32 (T line) and reference line 31 (C line), and detection line 32 and reference line 31 are arranged at the same face of fluorescent test paper 3 with interval.

[0046] Electronic fluorescent test paper disease detection pen sensor includes shell, control circuit module 12, display screen 11 and photoelectric detection module 22.Control circuit module 12 is respectively control connected with display screen 11 and photoelectric detection module 22 and power supply for display screen 11 and photoelectric detection module 22.

[0047] Wherein, the shell includes detachably connected upper shell 1 and lower shell 2, and the upper shell 1 is provided with accommodating cavity 16 and first connecting sliding groove 13, and the first connecting sliding groove 13 is communicated with the bottom of the accommodating cavity 16 and the upper shell 1.Control circuit module 12 is accommodated in accommodating cavity 16, specifically, right side positioning column 14 and left side positioning column 15 are formed in accommodating cavity 16, and control circuit module 12 is fixedly connected with accommodating cavity 16 by right side positioning column 14 and left side positioning column 15, and control circuit module 12 is not in contact with the side wall of accommodating cavity 16.Display screen 11 is arranged on the outer wall of upper shell 1.

[0048] The lower shell 2 is provided with a slide rail 21, a slide cavity 25 and a second connecting slide groove 26. The slide rail 21 is arranged on the side wall of the lower shell 2 along the extension direction of the lower shell 2. The slide cavity 25 and the second connecting slide groove 26 are cavities arranged along the extension direction of the lower shell 2. One end of the slide cavity 25 is in communication with the bottom of the lower shell 2 and forms a reagent insertion port 253 at the bottom. The other end of the slide cavity 25 is in communication with the second connecting slide groove 26 and is in communication with the top of the lower shell 2 through the second connecting slide groove 26. In addition, the slide cavity 25 is in communication with the slide rail 21 at the side and their lengths are substantially the same. The inner wall of the slide cavity 25 is provided with a first switch sensing module 251 and a second switch sensing module 253. The first switch sensing module 251 and the second switch sensing module 253 are internally provided with sensing sheets which can be in contact with the test paper 3 to sense and transmit the sensing signal to the control circuit module 12.

[0049] The photoelectric detection module 22 is slidably connected with the slide rail 21 and partially located in the slide cavity 25. The photoelectric detection module 22 can move up and down along the extension direction of the slide rail 21. Further, the slide rail 21 is provided with a limiting block 23 at the position close to the second connecting slide groove 26 at the upper end thereof, for limiting the movement range of the photoelectric detection module 22 and preventing it from entering and sliding out of the second connecting slide groove 26 from the lower shell. The lower end of the slide rail 21 is provided with a positioning block 24. When the fluorescent test paper 3 is inserted into the lower shell through the test paper insertion port 253, the photoelectric detection module 22 moves to the position abutting against the positioning block 24. At this time, the photoelectric detection module 22 can emit laser light to the reference line 31. Since there are various models of fluorescent test papers 3 on the market, when the position of the positioning block 24 is not aligned with the reference line 31, the light path can be adjusted by controlling the photoelectric detection module 22 to move up and down, so that the photoelectric detection module 22 is aligned with the reference line 31 or the detection line 32.

[0050] The detection circuit module 12 and the photoelectric detection module 22 are connected through wires which are stored in the hollow cavity formed by the slide cavity 25, the second connecting slide groove 26, the first connecting slide groove 13 and the accommodating cavity 16.

[0051] The specific structure of the photoelectric detection module 22 is as shown in Figure 3As shown, the photoelectric detection module 22 comprises a shell 221, a light source 222, a purple filter 223, a dichroic mirror 224, a red filter 225, and a photodiode 226. The shell 221 is provided with a first channel penetrating in a first direction (in this embodiment, the first direction is the extension direction of the slide rail) and a second channel perpendicular to the first channel, one end of the second channel is located on the surface of the shell 221, and the other end of the second channel communicates with the first channel. The dichroic mirror 224 is arranged at the intersection of the first channel and the second channel, and the included angle between the dichroic mirror 224 and the first channel and the second channel is 45°, the first channel is sequentially provided with the purple filter 223 and the light source 222 outward from the dichroic mirror 224, and the second channel is sequentially provided with the red filter 225 and the photodiode 226 outward from the dichroic mirror 224. The light source 222 emits a light beam with a wavelength of 365 nm, the purple filter 223 adopts a 365 nm band-pass filter, the dichroic mirror 224 adopts a 365 nm reflection and 615 nm transmission dichroic beam splitter, and the red filter 225 adopts a 615 nm band-pass filter.

[0052] The working principle of the photoelectric detection module 22 is as follows: the light source 222 emits 365 nm laser, filters other light except 365 nm through the purple filter 223, then irradiates downward through the dichroic mirror 224, irradiates to the surface of the fluorescent test paper 3, then the fluorescent test paper 3 is excited to emit 615 nm light, sequentially reflects to the second channel through the dichroic mirror 224, filters other light except 615 nm through the red filter 225, and finally irradiates to the photodiode 226.

[0053] The control circuit module 12 is provided with a power supply, a main control chip, an ADC conversion chip and a peripheral circuit, the peripheral circuit comprises a plurality of capacitors, resistors and transistors, the control circuit module 12 adopts an ultra-thin circuit board, the main control chip adopts an STM32F103C8T6 chip, the ADC conversion chip adopts an AD7686BRMZ chip, and the power supply adopts a rechargeable lithium battery.

[0054] Referring to Figure 4, the pin Key1 of the PB13 of the STM32F103C8T6 chip is the switch sensing signal input of the first switch sensing module, and the pin Key2 of the PB12 is the switch sensing signal input of the second switch sensing module; the pin OLED_SDA of the PB9 and the pin OLED_SCL of the PB8 are connected with the display screen wire, so that the main control chip controls the display screen; the pin AD_SDI of the PB3 is connected with the SDI pin of the AD7686BRMZ chip, the pin AD_SDO of the PB4 is connected with the SDO pin of the AD7686BRMZ chip, the pin AD_CNV of the PB0 is connected with the CNV pin of the AD7686BRMZ chip, and the pin AD_SCK of the PB1 is connected with the SCK pin of the AD7686BRMZ chip, so that the conversion from the analog signal transmitted by the photoelectric transmission module to the digital signal is realized, and the other capacitors are the conventional circuits for realizing the normal functions of the STM32F103C8T6 chip.

[0055] Referring to Figure 5 The AD7686BRMZ chip is a 16-bit charge redistribution successive approximation analog-to-digital converter, which adopts a single 5V power supply. The converter is built-in a low-power, high-speed, 16-bit no-code sampling ADC, has an internal conversion clock and a multifunctional serial interface port. The pin 1 REF is the reference voltage input and is connected with the positive pole of the power supply; the pin 2 VDD is connected with the positive pole of the power supply; the pin 3 IN+ is the analog signal input and is connected with the photoelectric diode, the analog signal is transmitted to the AD7686BRMZ chip through the Signal from the photoelectric diode, wherein the operational amplifier U5.2 constitutes a reverse amplification circuit to amplify the voltage emitted by the photoelectric diode, and the operational amplifier U5.1 constitutes a buffer to perform signal isolation; the pin 4 IN- is the analog input ground and is grounded; the pin 5 GND is grounded; the pin 6 CNV is the conversion input pin and is converted back and forth between high and low levels and reads data when the voltage drops; the pin 7 SDO is the serial data output and transmits the digital signal to the STM32F103C8T6 chip; the pin 8 SCK is the serial data clock input and inputs data at low level; the pin 9 SDI is the serial data input and is set to high level; and the pin 10 VIO is the input / output interface digital power supply and is connected with the positive pole of the power supply.

[0056] The working principle and use method of the electronic fluorescent test paper disease detection pen sensor provided in the embodiment are introduced as follows:

[0057] In this embodiment, the reference line 31 can be called C line, and the detection line 32 can be called T line. In the detection process, the C line is detected first, that is, if the C line has no display, it indicates that the detection is invalid, and the detection is stopped; if the C line has a display, the detection of the T line is carried out, and if the T line has a display, it indicates that the detection result is positive; if the C line has a display, the detection of the T line is carried out, and if the T line has no display, it indicates that the detection result is negative.

[0058] In actual application, the photoelectric detection module 22 is fixed on the fixed position of the slide rail 21 through the positioning block 24, waits for the fluorescent test paper 3 to pass through the reagent insertion port 253 to enter the slide cavity 25, and when the fluorescent test paper 3 passes through the first switch sensing module 251, the photoelectric detection module 22 is positioned above the reference line 31, the signal is transmitted to the control circuit module 12, the control circuit module 12 controls the photoelectric detection module 22 to start working through the wire, and at the same time, the control circuit module 12 controls the display screen 11 to display “start” through the wire, the photoelectric diode 226 converts the received light signal into a voltage analog signal and transmits it to the ADC conversion chip in the control circuit module 12, the ADC conversion chip converts the voltage analog signal into a digital signal and transmits it to the main control chip, and the main control chip processes the received signal; if the main control chip receives the excitation light signal, the main control chip controls the display screen 11 to display “valid, proceed to the next step”; if the main control chip does not receive the excitation light signal, the main control chip controls the display screen 11 to display “invalid”.

[0059] The user judges whether to further move the fluorescent test paper 3 in the slide cavity 25 according to the information displayed on the display screen, the photoelectric detection module 22 is positioned above the detection line 32 through the second switch sensing module 252, the signal is transmitted to the control circuit module 12, the control circuit module 12 controls the photoelectric detection module 22 to start working through the wire, and at the same time, the control circuit module 12 controls the display screen 11 to display “start of the second stage” through the wire, the photoelectric diode 226 converts the received light signal into a voltage analog signal and transmits it to the ADC conversion chip of the control circuit module 12, the ADC conversion chip converts the voltage analog signal into a digital signal and transmits it to the main control chip, and the main control chip processes the received signal; if the main control chip receives the excitation light signal, the main control chip controls the display screen 11 to display “positive”; if the main control chip does not receive the excitation light signal, the main control chip controls the display screen 11 to display “negative”.

[0060] Although the embodiments of the present application have been shown and described above, it should be understood that the above embodiments are exemplary and cannot be understood as limiting the present application, and those skilled in the art can make changes, modifications, replacements and variations to the above embodiments within the scope of the present application.

Claims

1. An electronic fluorescent test strip disease detection pen-type sensor for detecting a fluorescent test strip having a test line and a reference line, the test line and the reference line being disposed at the same face of the fluorescent test strip at intervals, characterized in that, The electronic fluorescent test paper disease detection pen sensor comprises a shell, a control circuit module, a display screen and a photoelectric detection module; The photoelectric detection module comprises a shell, a light source, a purple filter, a dichroic mirror, a red filter and a photodiode; The shell is provided with a first channel penetrating in a first direction and a second channel perpendicular to the first channel, one end of the second channel is located on the surface of the shell, and the other end of the second channel communicates with the first channel; The dichroic mirror is arranged at the intersection of the first channel and the second channel, and the included angle between the dichroic mirror and the first channel and the second channel is 45°, the first channel is sequentially provided with the purple filter and the light source outward from the dichroic mirror, and the second channel is sequentially provided with the red filter and the photodiode outward from the dichroic mirror; The shell has opposite first and second ends, a receiving cavity is formed in the first end, a slide rail is formed in the side wall of the second end along the extension direction of the shell, a test paper insertion port parallel to the slide rail is formed in the end face of the second end, and a slide cavity is formed in the second end and communicates with the receiving cavity, the slide rail and the test paper insertion port; The control circuit module is accommodated in the receiving cavity, the display screen is arranged on the outer wall of the shell, the photoelectric detection module is slidably connected with the slide cavity, the first direction is the extension direction of the slide rail, and the red filter is located between the photodiode and the slide cavity; The fluorescent test paper enters the slide cavity through the test paper insertion port, and the photoelectric detection module slides along the slide cavity so that the photoelectric detection module can be aligned with the detection line and the reference line of the fluorescent test paper of different lengths; The control circuit module is in control connection with the display screen, the photodiode and the light source and supplies power to the display screen, the photodiode and the light source.

2. The electronic fluorescent test strip disease detection pen sensor of claim 1, wherein, The first and second switch sensing modules are arranged in sequence on the inner wall of the slide cavity in the direction from the test paper insertion port to the receiving cavity; The first and second switch sensing modules are in control connection with the control circuit module and are supplied with power by the control circuit module; When the fluorescent test paper enters the slide cavity through the test paper insertion port and contacts the first or second switch sensing module, the first or second switch sensing module sends a signal to the control circuit module, and the control circuit module controls the light source to be turned on.

3. The electronic fluorescent test strip disease detection pen sensor according to claim 1 or 2, wherein, The emission wavelength of the light source is 365 nm, the purple filter is a 365 nm band-pass filter, the dichroic mirror is a dichroic beam splitter that reflects 365 nm light and transmits 615 nm light, and the red filter is a 615 nm band-pass filter.

4. The electronic fluorescent test strip disease detection pen sensor of claim 2, wherein, The control circuit module comprises a power supply, a main control chip and an ADC conversion chip. The ADC conversion chip is used for receiving the electric signal emitted by the photodiode, and then converting the electric signal into a digital signal and transmitting the digital signal to the main control chip; The main control chip is in control connection with the first switch induction module, the second switch induction module, the light source, the display screen and the ADC conversion chip; The power supply supplies power for the first switch induction module, the second switch induction module, the light source, the display screen, the ADC conversion chip and the main control chip.

5. The electronic fluorescent test strip disease detection pen sensor of claim 4, wherein, The main control chip is an STM32F103C8T6 chip, the ADC conversion chip is an AD7686BRMZ chip, and the power supply is a rechargeable lithium battery.

6. The electronic fluorescent test strip disease detection pen sensor of claim 1, wherein, The accommodating cavity is provided with two positioning columns, the control circuit module is fixedly connected with the accommodating cavity through the positioning columns, and the control circuit is not in contact with the side wall of the accommodating cavity.

7. The electronic fluorescent test strip disease detection pen sensor of claim 1, wherein, The shell comprises an upper shell and a lower shell which are detachably connected. The accommodating cavity is formed in the upper shell, and the slide rail, the slide cavity and the test paper insertion port are formed on the lower shell.

8. The electronic fluorescent test strip disease detection pen sensor of claim 7, wherein, The upper shell is further provided with a first connecting sliding groove which is in communication with the accommodating cavity and the bottom surface of the upper shell. The lower shell is further provided with a second connecting sliding groove which is in communication with the slide cavity and the top surface of the lower shell. When the upper shell and the lower shell are connected, the first connecting sliding groove and the second connecting sliding groove are in communication.

9. The electronic fluorescent test strip disease detection pen sensor of claim 8, wherein, The slide rail is provided with a limiting block at one end close to the second connecting sliding groove. When the photoelectric detection module slides to the top end of the slide rail, the limiting block is in abutment with the photoelectric detection module.

10. The electronic fluorescent test strip disease detection pen sensor of claim 1, wherein, The lower end of the slide rail is further provided with a positioning block. When the fluorescent test paper is inserted into the test paper insertion port and the photoelectric detection module is in abutment with the positioning block, the reference line is aligned with the second channel.

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