Device for assisting feeder cell preparation

Abstract

The utility model discloses a device for assisting feeder cell preparation, and relates to the technical field of test instruments, in particular to a device for assisting feeder cell preparation, which comprises a device body internally provided with an air pump and a ventilation interface communicated with the air pump through an air pipe; a control button is arranged on a holding part of the device body and is electrically connected with a control unit of the air pump; the top end of the transfer pipette is provided with an insertion port communicated with an inner cavity of the transfer pipette, and the ventilation port is inserted into the insertion port and is communicated with the inner cavity of the transfer pipette; a joint communicated with an inner cavity of the pipette is arranged at the bottom end of the pipette; the needle head is communicated with the connector in an inserted manner; according to the device for assisting in feeder cell preparation, the air pump is adopted to drive the pipette to suck and discharge liquid, the problem that a large-capacity pipette is used by a single hand is solved, and meanwhile, the needle head is additionally arranged in the pipette in a joint manner, so that the pipette has the puncturing capability, and a feeder cell preparation test is facilitated.

Description

Technical Field

[0001] This utility model relates to the field of experimental instrument technology, and more specifically, to a device for assisting in the preparation of feeder cells. Background Technology

[0002] Currently, in the experimental processes of chemistry, biology, medicine and other fields, especially in the in vitro diagnostic reagent industry, the preparation of feeder cells often involves the experimental operation of aspirating culture medium with syringes. The aseptic requirements for the preparation of feeder cells are high. Generally, in a clean bench, the experimenter needs to manually aspirate a large amount of culture medium into dozens or even hundreds of 10ml syringes with one hand, and then carry out the next experimental process of natural aseptic extraction of feeder cells.

[0003] Culture media are typically stored in sterile blue-mouth bottles. Routine laboratory personnel must hold the bottle with one hand and then use a syringe to aseptically aspirate the liquid with the same hand. This limits the use of large-volume syringes, necessitating the continuous operation of numerous small-volume syringes for aseptic liquid aspiration. This is not only time-consuming and laborious, causing swelling and pain in the operator's fingers, but also increases the risk of contamination due to unstable hand movements during single-handed application, potentially causing the sterile needle to touch the bottle opening. This is especially problematic during intraperitoneal injection in mice, where hand tremors can enlarge the peritoneal opening, allowing feeder cell-containing fluid to leak out, severely impacting the safety and speed of the experiment. Furthermore, prolonged reliance on the arm to support the weight increases wrist fatigue, reduces accuracy, and further increases the risk of contamination.

[0004] In summary, how to provide an auxiliary device for preparing feeder cells that can avoid excessive hand injury to the operator during the experiment and prevent contamination of the test samples or equipment is a problem that urgently needs to be solved by those skilled in the art. Utility Model Content

[0005] In view of this, the purpose of this utility model is to provide an auxiliary device for the preparation of feeder cells. It uses an air pump to drive the pipette for liquid aspiration and dispensing, which solves the problem of using a large-capacity pipette with one hand. At the same time, it uses a connector to install a needle inside the pipette, which enables it to puncture, thereby facilitating the preparation of feeder cells.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] An apparatus for assisting in the preparation of feeder cells, comprising:

[0008] The device body has a built-in air pump and an air inlet connected to the air pump via an air tube; the grip part of the device body is provided with a control button, which is electrically connected to the control unit of the air pump.

[0009] A pipette is provided with a plug interface at the top end of the pipette, which is connected to the inner cavity of the pipette, and is used for inserting the ventilation interface into the inner cavity of the pipette and making the inner cavity of the pipette and the ventilation interface communicate with each other; the bottom end of the pipette is provided with a joint connected to the inner cavity of the pipette;

[0010] A needle is inserted into the joint to make the joint and the needle communicate with each other.

[0011] Preferably, the ventilation interface is provided with a gas filter inside, which is used for filtering the gas flowing through the ventilation interface.

[0012] Preferably, the plug interface is provided with filter cotton inside, which is used for preventing the liquid in the pipette from entering the ventilation interface.

[0013] Preferably, the center line of the pipette is parallel to the center line of the holding part of the device body, and the center line of the needle forms an angle α with the center line of the pipette, 0° < α ≤ 90°.

[0014] Preferably, the top of the holding part of the device body is provided with a support hanging piece baffle, which is used for inhibiting the relative displacement between the device body and the holding hand of the operator and for hanging the device body on a gun rack.

[0015] Preferably, the device body is vertically provided with a support column on each side, and the axes of the support columns on the two sides overlap.

[0016] The device further comprises a support frame, which is provided with a clamping ring for lifting the support column.

[0017] Preferably, the clamping ring is arranged at the top of the support frame, and the clamping ring is a U-shaped structure with an open upper end.

[0018] Preferably, the bottom of the support frame is provided with a straight sliding rail capable of sliding in the horizontal direction, and the support frame is a telescopic rod with adjustable length.

[0019] Preferably, the support column is a cylinder, and is provided with an annular groove around the outer periphery, which is used for clamping the clamping ring.

[0020] Preferably, the control button comprises a liquid suction button and a liquid discharge button, and the stroke directions of the liquid suction button and the liquid discharge button are perpendicular to the axis of the support column.

[0021] Compared with the prior art, the device for assisting the preparation of feeding cells provided by the utility model has at least the following beneficial effects:

[0022] 1. The gas pump is integrated in the device body, negative pressure is generated in the pipette by the action of the gas pump to suck liquid, and positive pressure is generated in the pipette by the action of the gas pump to discharge liquid, thereby solving the problem that a large-capacity syringe cannot be operated with one hand, and avoiding the problem of hand strain caused by using a large number of small-capacity syringers during the test process;

[0023] 2. The gas pump is used to drive the liquid suction and discharge of the pipette, thereby reducing the amount of hand labor and avoiding the problem of hand shaking caused by hand fatigue, thereby avoiding the pollution problem caused by the contact between the needle and the medicine bottle;

[0024] 3. The pipette and the device body are connected by a plug-in interface, that is, the pipette and the device body are designed to be separable, and during the test, the capacity of the pipette can be selected according to the needs;

[0025] 4. The needle is connected and installed at the bottom end of the pipette through a connector, which facilitates puncture, and the needle can be replaced, thereby improving the applicability of the equipment. BRIEF DESCRIPTION OF DRAWINGS

[0026] In order to more clearly illustrate the technical scheme in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description. Obviously, the drawings in the following description are only embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor on the basis of the provided drawings.

[0027] Figure 1 The structure diagram of the device for assisting in the preparation of feeder cells provided by the present application is shown in the figure.

[0028] Figure 2 The side view of the device for assisting in the preparation of feeder cells provided by the present application is shown in the figure.

[0029] Figure 3 The structure diagram of the working state of the device for assisting in the preparation of feeder cells provided by the present application is shown in the figure.

[0030] In the figure:

[0031] 1. Device body; 2. Needle; 3. Pipette; 4. Liquid suction button; 5. Liquid discharge button; 6. Gas pump; 7. Ventilation interface; 8. Support hanging piece baffle; 9. Plug-in interface; 10. Connector; 11. Protective cap; 12. Support column; 13. Support frame. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the utility model.

[0033] The core of the utility model provides a device for assisting feeder cell preparation, using air pump to drive pipette to suck and discharge liquid, solving the problem of using large capacity pipette with one hand, and using joint to install needle in pipette, so that it has puncture ability, thereby facilitating feeder cell preparation test.

[0034] Please refer to Figures 1-3 A device for assisting feeder cell preparation, comprising:

[0035] The device body 1 is internally provided with an air pump 6 and an air interface 7 connected with the air pump 6 through an air pipe; the holding part of the device body 1 is provided with a control button, and the control button is electrically connected with the control unit of the air pump 6;

[0036] The pipette 3 is provided at the top end with a plug-in interface 9 connected with the inner cavity of the pipette 3, for inserting the air interface 7 and connecting with the inner cavity of the pipette 3; the bottom end of the pipette 3 is provided with a joint 10 connected with the inner cavity of the pipette 3;

[0037] The needle 2 is plugged in and connected with the joint 10;

[0038] As Figure 1 shown, by arranging the air pump 6 in the device body 1, the air pump 6 can generate negative pressure in the pipette 3 through positive action, so as to realize automatic liquid suction of the pipette 3, and the air pump 6 can also generate positive pressure in the pipette 3 through reverse action, so as to realize automatic liquid discharge of the pipette 3, that is, to solve the problem of difficult operation of the syringe with one hand;

[0039] And the pipette 3 and the device body 1 are connected in the plug-in interface 9 and the air interface 7, that is, the pipette 3 and the device body 1 are designed to be detachable, so that different specifications and capacities of pipettes 3 can be replaced according to the test needs, such as using large capacity pipette 3, which can suck a large amount of reagent at a time, thereby reducing the number of reagent suction, reducing the labor intensity of the experimental personnel, and reducing the labor loss of the experimental personnel;

[0040] The pipette 3 can also be replaced according to the different reagents used, such as using a pipette 3 made of polystyrene material, which can be used cyclically after sterilization, thereby reducing the use cost;

[0041] Meanwhile, the needle 2 is connected with the pipette 3 by using the universal connector 10, and different specifications of the needle 2 can be replaced according to the test requirements, or the needle 2 can be replaced according to different reagents; the position of the needle tip of the needle 2 is preferably stainless steel, and the position of the needle plug is polystyrene at the right end, so that the needle 2 can also be sterilized, thereby realizing recycling and reducing the use cost;

[0042] Meanwhile, the protective cap 11 is added outside the needle 2 to avoid accidental injury of the experimental personnel by the needle 2 when not in use or pollution or damage of the needle 2 caused by the needle 2 touching other objects.

[0043] In some embodiments, a filter piece is arranged in the venting interface 7 for filtering the gas flowing through the venting interface 7;

[0044] As shown in the drawings, Figure 1 A filter piece is added in the venting interface 7 to filter the gas entering the air pump 6 through the venting interface 7, so as to avoid bacteria or other impurities from entering the air pump 6, thereby avoiding the contaminated gas flow generated by the air pump 6 under positive pressure from entering the pipette 3 to pollute the reagent.

[0045] In some embodiments, filter cotton is arranged in the plug interface 9 to prevent the liquid in the pipette 3 from entering the venting interface 7;

[0046] As shown in the drawings, Figure 1 The plug interface 9 is arranged at the top of the pipette 3, so that when the reagent in the pipette 3 does not exceed the maximum capacity, it will not overflow through the plug interface 9, and filter cotton is arranged at the plug interface 9 to filter the gas passing through the plug interface 9 and absorb the moisture in the gas, thereby avoiding the entry of humid gas into the air pump 6.

[0047] In some embodiments, the center line of the pipette 3 is parallel to the center line of the holding portion of the device body 1, and the center line of the needle 2 and the center line of the pipette 3 form an angle α, 0° < α ≤ 90°;

[0048] As shown in the drawings, Figure 1 The device body 1 adopts a columnar structure as a whole, and a bending portion extending forward is arranged at the lower end of the holding portion, but the center line of the venting interface 7 at the bottom of the device body 1 is parallel to the center line of the holding portion, so that the pipette 3 can move forward to avoid the visual blind area caused by the shielding of the operator's arm;

[0049] Meanwhile, the pipette 3 also adopts a transparent circular tube structure, and scale lines are arranged on the tube wall for observing the actual liquid volume of the pipette 3;

[0050] By restricting the angles of the pipette 3, the gripping part of the device body 1, and the needle 2, the experimenter can hold the device body 1 in a more comfortable posture. Specifically, the gripping part of the device body 1 is held in a fist with the index finger on top and the little finger at the bottom. This reduces wrist fatigue and ensures that the experimenter has a good field of vision of the position of the needle 2 after holding it, which facilitates the puncture operation of the needle 2.

[0051] In some embodiments, a support bracket 8 is provided on the top of the gripping portion of the device body 1 to suppress relative displacement between the device body 1 and the operator's gripping hand and to suspend the device body 1 on the gun rack.

[0052] like Figure 1 As shown, a support bracket 8 is provided on the top of the gripping part of the device body 1. The front end of the support bracket 8 is in the shape of a hook, which makes it easy to hang the entire device on the gun rack. At the same time, the main body of the support bracket 8 can contact the index finger of the tester. That is, when the tester holds it, the relative sliding between the hand and the gripping part is restricted, so that the device has the function of preventing the hand from slipping.

[0053] In some embodiments, support columns 12 are respectively vertically arranged on both sides of the device body 1, and the axes of the support columns 12 on both sides overlap.

[0054] It also includes a support frame 13, which has a retaining ring inside for supporting the support column 12;

[0055] like Figure 2 As shown, by setting the support column 12 and the support frame 13, the device body 1 can be supported by the support frame 13. During operation, as... Figure 3 As shown, during the liquid aspiration stage, the device body 1 can be supported on the support frame 13, thereby reducing the burden on the wrist and ensuring the overall stability of the device body 1 and the needle 2, thus avoiding contamination caused by the needle 2 touching the medicine bottle.

[0056] In some embodiments, the retaining ring is disposed on the top of the support frame 13, and the retaining ring is a U-shaped structure with an open top.

[0057] like Figure 1 As shown, the retaining ring is set on the top of the support frame 13, and the retaining ring has a U-shaped structure. When the support column 12 is engaged in the retaining ring, the support column 12 can rotate in the U-shaped retaining ring, that is, the angle of the needle 2 can be adjusted, which facilitates aspiration and drainage after puncture.

[0058] In some embodiments, the bottom of the support frame 13 is provided with a linear slide rail that can slide in the horizontal direction, and the support frame 13 is a telescopic rod with adjustable length.

[0059] A linear slide rail is added to the bottom of the support frame 13. When performing puncture, the angle of the needle 2 is adjusted to be parallel to the linear slide rail. The needle 2 can be driven to perform puncture by moving the support frame 13. Throughout the process, the device body 1 is always supported by the support frame 13, thereby reducing wrist strain. Or, when the experimenter does not need the support frame 13, the support frame 13 can be slid to other positions under the action of the linear slide rail to avoid interfering with the experiment.

[0060] Meanwhile, the support frame 13 adopts a telescopic rod structure design, which can be height adjusted so that the experimenters can adjust it according to their own operating habits, thereby reducing the fatigue during the experiment.

[0061] In some embodiments, the support column 12 is a cylinder and has an annular groove on its outer periphery for engaging a retaining ring.

[0062] By designing the support column 12 as a cylinder, the resistance when the support column 12 rotates relative to the retaining ring is reduced. At the same time, an annular groove is provided on the outer periphery of the support column 12, which helps to engage the retaining ring in the annular groove to limit the axial movement of the support column 12.

[0063] In some embodiments, the control buttons include a suction button 4 and a drain button 5, the travel directions of the suction button 4 and the drain button 5 being perpendicular to the axis of the support column 12.

[0064] like Figure 1 As shown, the travel direction of the suction button 4 and the discharge button 5 is set to facilitate the force exerted by the fingers after the experimenter holds the object.

[0065] The specific usage procedure of this auxiliary device for preparing feeder cells is as follows: the experimenter holds the grip part of the device body 1. When holding it, the index or middle finger is in a position that can press the liquid aspiration button 4 and the liquid drainage button 5, and at the same time, the support column 12 is locked in the retaining ring of the support frame 13.

[0066] During the liquid aspiration stage, insert the needle 2 into the medicine bottle, press the liquid aspiration button 4, and the air pump 6 will generate negative pressure, allowing the medicine to enter the pipette 3 through the needle 2;

[0067] During the drainage stage, needle 2 punctures the mouse peritoneum, and the drainage button 5 is pressed. The air pump 6 generates positive pressure, and the drug enters the mouse peritoneal cavity through needle 2. Needle 2 is removed, and the mouse peritoneal cavity is pressed to mix the drug inside. Then, needle 2 punctures the mouse peritoneum again, and the aspiration button 4 is pressed to aspirate the solution from the mouse peritoneal cavity into the pipette 3 through needle 2.

[0068] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0069] The device for assisting in preparation of feeding cells is described in detail above. The principle and implementation mode of the device are described by using specific examples in this paper, and the above examples are only used to help understand the method and core idea of the device. It should be pointed out that for ordinary skilled persons in the technical field, some improvements and modifications can be made to the device without departing from the principle of the device, and these improvements and modifications also fall within the protection scope of the claims of the device.

Claims

1. An apparatus for assisting in the preparation of feeder cells, characterized in that, The device comprises: a device body (1) with a gas pump (6) and a ventilation interface (7) connected to the gas pump (6) through a gas pipe; the holding part of the device body (1) is provided with a control button which is electrically connected to the control unit of the gas pump (6); a pipette (3) with a plug-in interface (9) at the top end for connecting the inner cavity of the pipette (3) to the ventilation interface (7); the bottom end of the pipette (3) is provided with a connector (10) for connecting the inner cavity of the pipette (3); a needle (2) connected to the connector (10).

2. The device for aiding preparation of feeder cells according to claim 1, wherein The ventilation interface (7) is provided with a gas filter for filtering the gas flowing through the ventilation interface (7).

3. The device for assisting in the preparation of feeder cells according to claim 1, wherein: The plug-in interface (9) is provided with filter cotton to prevent liquid in the pipette (3) from entering the ventilation interface (7).

4. The device for aiding preparation of feeder cells according to claim 1, wherein The center line of the pipette (3) is parallel to the center line of the holding part of the device body (1), and the center line of the needle (2) forms an angle α with the center line of the pipette (3), 0° < α ≤ 90°.

5. The device for aiding preparation of feeder cells according to claim 1, wherein The top of the holding part of the device body (1) is provided with a support hanger baffle (8) for preventing the relative displacement of the device body (1) and the operator's holding hand and for hanging the device body (1) on a gun rack.

6. The device for assisting in the preparation of cell feedstock according to any one of claims 1 to 5, wherein The device body (1) is provided with a support column (12) on each side, and the axes of the two support columns (12) overlap. The device further comprises a support frame (13) provided with a clamping ring for lifting the support column (12).

7. The device for aiding preparation of feeder cells according to claim 6, wherein The clamping ring is arranged at the top of the support frame (13) and has a U-shaped structure with an open upper end.

8. The device for aiding preparation of feeder cells according to claim 6, wherein The bottom of the support frame (13) is provided with a straight sliding rail which can slide in the horizontal direction, and the support frame (13) is an extendable rod with adjustable length.

9. The device for aiding preparation of feeder cells according to claim 6, wherein The support column (12) is a cylinder provided with an annular groove on the outer periphery for clamping the clamping ring.

10. The device for aiding preparation of feeder cells according to claim 6, wherein The control button comprises a liquid suction button (4) and a liquid discharge button (5), and the stroke direction of the liquid suction button (4) and the liquid discharge button (5) is perpendicular to the axis of the support column (12).

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