Liquid nitrogen quick-freezing centrifugal tube rack

Abstract

A liquid nitrogen quick-freezing centrifuge tube rack comprises a stainless steel rectangular frame body and two rows of fixing column sets arranged in the rectangular frame body, the two rows of fixing column sets correspond to the two long edges of the rectangular frame body respectively, each fixing column set is composed of a plurality of fixing columns distributed along the long edges of the rectangular frame body, and insertion holes allowing centrifuge tubes to be inserted are formed in the fixing columns. The fixing columns are connected with the inner side of the rectangular frame body through the connecting rib plates; the long edge of the rectangular frame body is provided with a pressing rod corresponding to each fixing column, the pressing rod is L-shaped and comprises a vertical part and a transverse part, the vertical part is fixed on the side edge of the rectangular frame body, one end of the transverse part is connected with the upper end of the vertical part, and the other end of the transverse part points to the upper part of the inserting hole. After the centrifugal tube is inserted into the insertion hole, the centrifugal tube is locked or unlocked in a rotating mode, and operation is convenient and fast; the centrifugal tube and the rectangular frame body are fixed together, so that the centrifugal tube can be immersed into liquid nitrogen in a vertical state, and uniform quick freezing of the centrifugal tube is ensured.

Description

TECHNICAL FIELD

[0001] The utility model relates to experimental apparatus, especially a liquid nitrogen quick freezing centrifugal tube frame. BACKGROUND

[0002] The sampling step of taking cell or tissue sample to do sequencing or omics includes: 1) the treated cell or tissue sample is divided into centrifugal tubes; 2) the centrifugal tube containing the cell or tissue sample is placed in liquid nitrogen for quick freezing for 5-15 min; 3) the centrifugal tube is transferred to a-80 DEG C refrigerator for storage; 4) subsequent experiment. At present, the centrifugal tube adopts EP tube made of plastic, and the centrifugal tube will float in liquid nitrogen, resulting in uneven quick freezing. At present, in order to solve the problem of centrifugal tube floating, the experimenter immerses the centrifugal tube in liquid nitrogen with forceps, which is not only troublesome but also unsafe. SUMMARY

[0003] The utility model wants to solve the technical problem to provide a kind of liquid nitrogen quick freezing centrifugal tube frame, can realize the sinking of centrifugal tube, and it is easy and safe to operate.

[0004] To solve the above technical problem, the technical scheme of the utility model is: a kind of liquid nitrogen quick freezing centrifugal tube frame, including the rectangular frame of stainless steel and two rows of fixed column groups being located in rectangular frame, two rows of fixed column groups are respectively with the two long sides of rectangular frame correspond, the fixed column group is made of several fixed columns along the long side distribution of rectangular frame, the fixed column is equipped with the insertion hole for the insertion of centrifugal tube, the fixed column is connected with the inside of rectangular frame by connecting rib plate;The long side of the rectangular frame is equipped with pressure bar corresponding each fixed column position, the pressure bar is L-shaped, it includes vertical portion and horizontal portion, the vertical portion is fixed in the side of rectangular frame, the horizontal portion is connected with the upper end of vertical portion, the other end of horizontal portion points to the upper of insertion hole.The utility model principle: first, centrifugal tube is inserted into the insertion hole of fixed column, rotates centrifugal tube, so that the protruding buckle part on the tube cover of centrifugal tube can be in contact with the horizontal portion of pressure bar, prevent centrifugal tube from being taken out of insertion hole upward;Then, rectangular frame is placed in liquid nitrogen together with centrifugal tube to carry out quick freezing, since centrifugal tube is under the constraint of pressure bar, it can be kept vertically under liquid surface, ensure that quick freezing is uniform;When the next batch of centrifugal tube quick freezing is needed, rectangular frame is lifted from liquid nitrogen first, after centrifugal tube leaves liquid nitrogen, rotate centrifugal tube by a certain angle to make buckle part be out of the constraint of pressure bar, then the quick frozen centrifugal tube is extracted upward from insertion hole, finally, new centrifugal tube is placed in insertion hole.

[0005] As an improvement, the height of the fixed column is less than the height of the centrifugal tube,

[0006] As an improvement, the buckle part on the tube cover of the centrifugal tube placed in the insertion hole can be in contact with the horizontal portion.

[0007] As an improvement, the end of the horizontal portion is provided with a ball.

[0008] As improvement, the bottom of the rectangular frame is provided with a supporting column.

[0009] As improvement, the two short sides of the rectangular frame are provided with a handle.

[0010] The present application has the following beneficial effects compared with the prior art:

[0011] After the centrifuge tube is inserted into the insertion hole, it is locked or unlocked through rotation, which is convenient and fast; the centrifuge tube and the rectangular frame are fixed together, so that the centrifuge tube can be kept in an upright state and immersed in liquid nitrogen, thereby ensuring uniform rapid freezing of the centrifuge tube. BRIEF DESCRIPTION OF DRAWINGS

[0012] Fig. 1 It is a perspective view of the combination of the centrifuge tube and the tube rack.

[0013] Fig. 2 It is a side view of the combination of the centrifuge tube and the tube rack.

[0014] Fig. 3 It is a schematic view of the centrifuge tube.

[0015] Fig. 4 It is a schematic view of the tube rack. DETAILED DESCRIPTION

[0016] The present application will be further described below in conjunction with the drawings of the specification.

[0017] For example, Figs. 1 to 4As shown, a liquid nitrogen quick-freezing centrifuge tube rack, the centrifuge tube 1 of the embodiment is an EP tube, which comprises a tube body 13 and a tube cover 11 matched with the tube body 13, the tube cover 11 comprises a cover plate and a round tube arranged at the bottom of the cover plate, the tube cover 11 is tightly matched with the opening of the tube body 13 through the round tube, the cover plate comprises a circular part with a diameter similar to that of the tube body 13 and a buckle part 12 protruding on one side of the circular part, and the tube cover 11 is usually opened or closed through the buckle part 12. The centrifuge tube rack comprises a rectangular frame 2 made of stainless steel and two rows of fixed column groups arranged in the rectangular frame 2, the two rows of fixed column groups correspond to the two long sides of the rectangular frame 2 respectively, the fixed column group is composed of a plurality of fixed columns 5 distributed along the long side of the rectangular frame 2, and the fixed columns 5 in the rectangular frame 2 of the embodiment are distributed in two rows and four columns in a rectangular shape; the fixed columns 5 are connected with the inner side of the rectangular frame 2 through connecting rib plates 6, the connecting rib plates 6 are arranged longitudinally and transversely, and the connecting rib plates 6 can reduce the weight of the whole rack; the fixed columns 5 are provided with insertion holes 51 for inserting the centrifuge tubes 1, the diameters of different positions of the centrifuge tubes 1 are different, and the centrifuge tubes 1 are clamped in the insertion holes 51 through the change of the diameters; the height of the fixed column is less than the height of the centrifuge tube 1, and when the centrifuge tube 1 is completely inserted into the insertion hole 51, the lower end of the centrifuge tube 1 is stretched out, so that the liquid nitrogen can surround a larger area of the centrifuge tube 1; the bottom of the rectangular frame 2 is provided with support columns 7, the rectangular frame 2 is supported through the support columns 7, and the bottom of the rectangular frame 2 has enough space for the lower end of the centrifuge tube 1 to stretch out; the two short sides of the rectangular frame 2 are provided with handles 8, when the rectangular frame 2 needs to be taken out from the liquid nitrogen, the handles 8 on both sides are pulled and lifted by using a drag hook. A pressing rod 3 is arranged on the long side of the rectangular frame 2 corresponding to the position of each fixed column, the pressing rod 3 is L-shaped, which comprises a vertical part and a horizontal part, the vertical part is fixed on the side of the rectangular frame 2, one end of the horizontal part is connected with the upper end of the vertical part, the other end of the horizontal part points to the upper side of the insertion hole 51 and extends to the buckle part 12, only when the buckle part 12 is rotated to the position below the end of the horizontal part, the centrifuge tube 1 can be locked in the insertion hole 51 by the pressing rod 3, otherwise, the centrifuge tube 1 can be easily pulled out of the insertion hole 51; a ball 4 is arranged at the end of the horizontal part, the buckle part 12 is pressed tightly through the ball 4, the effect of pressing is better, and it is convenient to rotate the buckle part 12 to the bottom of the ball; in order to achieve accurate fixing, a limiting baffle can be added to the rectangular frame 2 to limit the rotation angle of the centrifuge tube 1.

[0018] The utility model discloses principles: first, insert the centrifugal tube 1 into the insertion hole 51 of fixed column, rotate centrifugal tube 1, make the protruding buckle part 12 on the pipe cover 11 of centrifugal tube 1 can be with the transverse portion of pressure bar 3, prevent centrifugal tube 1 from being taken out insertion hole 51 upward; then put rectangular frame body 2 together with centrifugal tube 1 into liquid nitrogen and carry out quick freezing, because the constraint of pressure bar 3 to centrifugal tube 1, make it can be kept under the liquid level vertically, ensure quick freezing uniformity, when needing to change the next batch centrifugal tube 1 quick freezing, first, rectangular frame body 2 is lifted from liquid nitrogen, after centrifugal tube 1 leaves liquid nitrogen, rotate centrifugal tube 1 certain angle and make buckle part 12 separate the constraint of pressure bar 3, then take out centrifugal tube 1 after quick freezing from insertion hole 51 upward, finally put new centrifugal tube 1 in insertion hole 51 can.

Claims

1. A liquid nitrogen quick-freezing centrifuge tube rack, characterized in that: The device includes a stainless steel rectangular frame and two rows of fixing posts within the frame. The two rows of fixing posts correspond to the two long sides of the rectangular frame. Each fixing post consists of several posts distributed along the long side of the rectangular frame. Each fixing post has an insertion hole for inserting a centrifuge tube. The fixing posts are connected to the inner side of the rectangular frame via connecting ribs. A pressure bar is provided on the long side of the rectangular frame corresponding to each fixing post. The pressure bar is L-shaped and includes a vertical part and a horizontal part. The vertical part is fixed to the side of the rectangular frame, one end of the horizontal part is connected to the upper end of the vertical part, and the other end of the horizontal part points upwards from the insertion hole.

2. The liquid nitrogen quick-freezing centrifuge tube rack according to claim 1, characterized in that: The height of the fixed column is less than the height of the centrifuge tube.

3. The liquid nitrogen quick-freezing centrifuge tube rack according to claim 1, characterized in that: The latch on the cap of the centrifuge tube inside the insertion hole can abut against the transverse part.

4. The liquid nitrogen quick-freezing centrifuge tube rack according to claim 1, characterized in that: The end of the transverse portion is provided with a sphere.

5. The liquid nitrogen quick-freezing centrifuge tube rack according to claim 1, characterized in that: The bottom of the rectangular frame is provided with a support column.

6. The liquid nitrogen quick-freezing centrifuge tube rack according to claim 1, characterized in that: The rectangular frame is provided with handles on its two short sides.

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